1 Q0010 Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF Q0017 2 Q0017 Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF Q0010 3 Q0032 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4 Q0045 COX1 Subunit I of cytochrome c oxidase (Complex IV) Subunit I of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 5 Q0050 AI1 Reverse transcriptase required for splicing of the COX1 pre-mRNA Reverse transcriptase required for splicing of the COX1 pre-mRNA; encoded by a mobile group II intron within the mitochondrial COX1 gene C IMP mitochondrion GO_0005739 6 Q0055 AI2 Reverse transcriptase required for splicing of the COX1 pre-mRNA Reverse transcriptase required for splicing of the COX1 pre-mRNA; encoded by a mobile group II intron within the mitochondrial COX1 gene C IDA mitochondrion GO_0005739 7 Q0060 AI3 Endonuclease I-SceIII Endonuclease I-SceIII; encoded by a mobile group I intron within the mitochondrial COX1 gene 8 Q0065 AI4 Endonuclease I-SceII Endonuclease I-SceII; encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity 9 Q0070 AI5_ALPHA Endonuclease I-SceIV Endonuclease I-SceIV; involved in intron mobility; encoded by a mobile group I intron within the mitochondrial COX1 gene 10 Q0075 AI5_BETA Protein of unknown function Protein of unknown function; encoded within an intron of the mitochondrial COX1 gene; translational initiation codon is predicted to be ATA rather than ATG 11 Q0080 ATP8 Subunit 8 of the F0 sector of mitochondrial F1F0 ATP synthase Subunit 8 of the F0 sector of mitochondrial F1F0 ATP synthase; encoded on the mitochondrial genome; ATP8 and ATP6 mRNAs are not translated in the absence of the F1 sector of ATPase C HDA; IMP mitochondrion; mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) GO_0005739; GO_0000276 12 Q0085 ATP6 Subunit a of the F0 sector of mitochondrial F1F0 ATP synthase Subunit a of the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrially encoded; translation is specifically activated by Atp22p; ATP6 and ATP8 mRNAs are not translated in the absence of the F1 sector of ATPase C HDA; IDA mitochondrion; mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) GO_0005739; GO_0000276 13 Q0092 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 14 Q0105 COB Cytochrome b Cytochrome b; mitochondrially encoded subunit of the ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p C IDA; HDA mitochondrial respiratory chain complex III; mitochondrion GO_0005750; GO_0005739 15 Q0110 BI2 Mitochondrial mRNA maturase with a role in splicing Mitochondrial mRNA maturase with a role in splicing; encoded by both exon and intron sequences of partially processed COB mRNA 16 Q0115 BI3 Mitochondrial mRNA maturase Mitochondrial mRNA maturase; forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA 17 Q0120 BI4 Mitochondrial mRNA maturase Mitochondrial mRNA maturase; forms a complex with Nam2p to mediate splicing of the bI4 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA C IDA mitochondrion GO_0005739 18 Q0130 OLI1 F0-ATP synthase subunit c (ATPase-associated proteolipid) F0-ATP synthase subunit c (ATPase-associated proteolipid); encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2 C IPI; HDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion GO_0000276; GO_0005739 19 Q0140 VAR1 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane; may have a role in loss of mitochondrial DNA under stress conditions C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 20 Q0142 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious open reading frame Q0143 21 Q0143 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 22 Q0144 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 23 Q0160 SCEI I-SceI DNA endonuclease I-SceI DNA endonuclease; encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene 24 Q0182 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 25 Q0250 COX2 Subunit II of cytochrome c oxidase (Complex IV) Subunit II of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits C IPI; HDA mitochondrial respiratory chain complex IV; mitochondrion GO_0005751; GO_0005739 26 Q0255 Q0255 Maturase-like protein Maturase-like protein 27 Q0275 COX3 Subunit III of cytochrome c oxidase (Complex IV) Subunit III of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 28 Q0297 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RPM1 29 YAL001C TFC3 Subunit of RNA polymerase III transcription initiation factor complex Subunit of RNA polymerase III transcription initiation factor complex; part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding; largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC) C IDA; HDA; IDA transcription factor TFIIIC complex; mitochondrion; nuclear condensin complex GO_0000127; GO_0005739; GO_0000799 30 YAL002W VPS8 Membrane-binding component of the CORVET complex Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif C IDA; IDA; IDA late endosome; CORVET complex; membrane GO_0005770; GO_0033263; GO_0016020 31 YAL003W EFB1 Translation elongation factor 1 beta Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site C IMP eukaryotic translation elongation factor 1 complex GO_0005853 32 YAL004W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C 33 YAL005C SSA1 ATPase involved in protein folding and NLS-directed nuclear transport ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils C HDA; IDA; IDA; IDA; HDA; IDA; IPI; HDA nucleus; cytoplasm; fungal-type cell wall; fungal-type vacuole membrane; plasma membrane; polysome; chaperonin-containing T-complex; cytosol GO_0005634; GO_0005737; GO_0009277; GO_0000329; GO_0005886; GO_0005844; GO_0005832; GO_0005829 34 YAL007C ERP2 Member of the p24 family involved in ER to Golgi transport Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; ER to Golgi transport vesicle GO_0005783; GO_0030134 35 YAL008W FUN14 Integral mitochondrial outer membrane (MOM) protein Mitochondrial protein of unknown function C HDA; HDA; IDA mitochondrial outer membrane; mitochondrion; integral component of mitochondrial outer membrane GO_0005741; GO_0005739; GO_0031307 36 YAL009W SPO7 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation C HDA; IDA; IPI endoplasmic reticulum; integral component of membrane; Nem1-Spo7 phosphatase complex GO_0005783; GO_0016021; GO_0071595 37 YAL010C MDM10 Subunit of both the ERMES and the SAM complex Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase C IPI; IPI; HDA mitochondrial sorting and assembly machinery complex; ERMES complex; mitochondrial outer membrane GO_0001401; GO_0032865; GO_0005741 38 YAL011W SWC3 Protein of unknown function Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae C IDA; HDA; HDA Swr1 complex; mitochondrion; nucleus GO_0000812; GO_0005739; GO_0005634 39 YAL012W CYS3 Cystathionine gamma-lyase Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 40 YAL013W DEP1 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation C IDA; HDA Rpd3L complex; Rpd3L-Expanded complex GO_0033698; GO_0070210 41 YAL014C SYN8 Endosomal SNARE related to mammalian syntaxin 8 Endosomal SNARE related to mammalian syntaxin 8 C IDA endosome GO_0005768 42 YAL015C NTG1 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication C IDA; HDA nucleus; mitochondrion GO_0005634; GO_0005739 43 YAL016C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF TPD3/YAL016W 44 YAL016C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 45 YAL016W TPD3 Regulatory subunit A of the heterotrimeric PP2A complex Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III C IDA; IDA; IDA; IDA; IDA; IDA; IDA cytoplasm; cellular bud neck; mating projection tip; cellular bud tip; protein phosphatase type 2A complex; nucleus; spindle pole body GO_0005737; GO_0005935; GO_0043332; GO_0005934; GO_0000159; GO_0005634; GO_0005816 46 YAL017W PSK1 PAS domain-containing serine/threonine protein kinase PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 47 YAL018C LDS1 Protein Involved in spore wall assembly Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants C HDA; IDA; IDA lipid particle; ascospore wall; prospore membrane GO_0005811; GO_0005619; GO_0005628 48 YAL019W FUN30 Snf2p family member with ATP-dependent chromatin remodeling activity Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate C HDA; IPI; HDA; IDA nucleus; mating-type region heterochromatin; mitochondrion; chromosome, centromeric region GO_0005634; GO_0031934; GO_0005739; GO_0000775 49 YAL019W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF ATS1/YAL020C 50 YAL020C ATS1 Protein required for modification of wobble nucleosides in tRNA Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle C IPI cytoplasm GO_0005737 51 YAL021C CCR4 Component of the CCR4-NOT transcriptional complex Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening C IDA; IDA; IPI; IDA cytoplasm; P-body; Cdc73/Paf1 complex; CCR4-NOT core complex GO_0005737; GO_0000932; GO_0016593; GO_0030015 52 YAL022C FUN26 High affinity, broad selectivity, nucleoside/nucleobase transporter High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member C IDA; HDA; IDA membrane; fungal-type vacuole membrane; intracellular GO_0016020; GO_0000329; GO_0005622 53 YAL023C PMT2 Protein O-mannosyltransferase of the ER membrane Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication C IDA; IDA; HDA dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex; endoplasmic reticulum GO_0097582; GO_0097584; GO_0005783 54 YAL024C LTE1 Protein similar to GDP/GTP exchange factors Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures C IDA cellular bud GO_0005933 55 YAL025C MAK16 Essential nuclear protein Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus C IDA; IDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 56 YAL026C DRS2 Trans-golgi network aminophospholipid translocase (flippase) Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; subject to auto-inhibition by its C-terminal tail; mutations in human homolog ATP8B1 result in liver disease C IDA; HDA; IPI trans-Golgi network; endoplasmic reticulum; Cdc50p-Drs2p complex GO_0005802; GO_0005783; GO_1990530 57 YAL026C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAL027W and the verified gene DRS2 58 YAL027W SAW1 5'- and 3'-flap DNA binding protein 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus C HDA nucleus GO_0005634 59 YAL028W FRT2 Tail-anchored ER membrane protein of unknown function Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 60 YAL029C MYO4 Type V myosin motor involved in actin-based transport of cargos Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication C IDA; HDA; IDA; IDA cellular bud; mitochondrion; cellular bud tip; filamentous actin GO_0005933; GO_0005739; GO_0005934; GO_0031941 61 YAL030W SNC1 Vesicle membrane receptor protein (v-SNARE) Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; HDA; IDA; IDA; IDA cellular bud neck; endosome; cellular bud; transport vesicle membrane; cell periphery; SNARE complex; plasma membrane; trans-Golgi network GO_0005935; GO_0005768; GO_0005933; GO_0030658; GO_0071944; GO_0031201; GO_0005886; GO_0005802 62 YAL031C GIP4 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate C HDA cytoplasm GO_0005737 63 YAL031W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF GIP4/YAL031C 64 YAL032C PRP45 Protein required for pre-mRNA splicing Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene C IDA; IDA; IDA; IDA nucleus; U2-type catalytic step 2 spliceosome; U2-type catalytic step 1 spliceosome; post-mRNA release spliceosomal complex GO_0005634; GO_0071007; GO_0071006; GO_0071014 65 YAL033W POP5 Subunit of both RNase MRP and nuclear RNase P Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs C IPI; IDA ribonuclease MRP complex; nucleolar ribonuclease P complex GO_0000172; GO_0005655 66 YAL034C FUN19 Non-essential protein of unknown function Non-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication 67 YAL034C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 68 YAL034W-A MTW1 Essential component of the MIND kinetochore complex Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) C IDA; IDA spindle pole; nuclear MIS12/MIND complex GO_0000922; GO_0000818 69 YAL035W FUN12 Translation initiation factor eIF5B Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 C IDA; IDA; HDA; HDA; HDA; IDA; HDA cytosolic small ribosomal subunit; eukaryotic 48S preinitiation complex; cytoplasm; cytosol; mitochondrion; cytoplasmic stress granule; preribosome, large subunit precursor GO_0022627; GO_0033290; GO_0005737; GO_0005829; GO_0005739; GO_0010494; GO_0030687 70 YAL036C RBG1 Member of the DRG family of GTP-binding proteins Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid C IDA; IDA; IDA; HDA polysome; cytoplasmic stress granule; polysomal ribosome; cytoplasm GO_0005844; GO_0010494; GO_0042788; GO_0005737 71 YAL037C-A YAL037C-A Putative protein of unknown function Putative protein of unknown function 72 YAL037C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 73 YAL037W YAL037W Putative protein of unknown function Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication 74 YAL038W CDC19 Pyruvate kinase Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication C IDA; HDA cytoplasm; plasma membrane GO_0005737; GO_0005886 75 YAL039C CYC3 Cytochrome c heme lyase (holocytochrome c synthase) Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) C HDA; IDA mitochondrion; mitochondrial intermembrane space GO_0005739; GO_0005758 76 YAL040C CLN3 G1 cyclin involved in cell cycle progression G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry. C IDA nucleus GO_0005634 77 YAL041W CDC24 Guanine nucleotide exchange factor (GEF) for Cdc42p Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress C IDA; IDA; HDA; IDA; IDA; IDA nucleus; cellular bud neck; cytoplasm; mating projection tip; incipient cellular bud site; cellular bud tip GO_0005634; GO_0005935; GO_0005737; GO_0043332; GO_0000131; GO_0005934 78 YAL042C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene 79 YAL042W ERV46 Protein localized to COPII-coated vesicles Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport C HDA; IDA; IDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane; integral component of Golgi membrane; ER to Golgi transport vesicle GO_0005783; GO_0030176; GO_0030173; GO_0030134 80 YAL043C PTA1 Subunit of holo-CPF Subunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII C IDA; IPI mRNA cleavage and polyadenylation specificity factor complex; mRNA cleavage factor complex GO_0005847; GO_0005849 81 YAL044C GCV3 H subunit of the mitochondrial glycine decarboxylase complex H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF C IDA mitochondrion GO_0005739 82 YAL044W-A BOL1 Mitochondrial matrix protein involved in Fe-S cluster biogenesis Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein C IDA mitochondrial matrix GO_0005759 83 YAL045C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A 84 YAL046C BOL3 Protein involved in Fe-S cluster transfer to mitochondrial clients Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss C IDA mitochondrial matrix GO_0005759 85 YAL047C SPC72 Gamma-tubulin small complex (gamma-TuSC) receptor Component of the cytoplasmic Tub4p (gamma-tubulin) complex; binds spindle pole bodies and links them to microtubules; is regulated by Cdc5 kinase; has roles in astral microtubule formation and stabilization C IDA outer plaque of spindle pole body GO_0005824 86 YAL047W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF GEM1/YAL048C 87 YAL048C GEM1 Outer mitochondrial membrane GTPase, subunit of the ERMES complex Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases C IPI; HDA; HDA ERMES complex; mitochondrion; mitochondrial outer membrane GO_0032865; GO_0005739; GO_0005741 88 YAL049C AIM2 Cytoplasmic protein involved in mitochondrial function or organization Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor C HDA cytoplasm GO_0005737 89 YAL051W OAF1 Oleate-activated transcription factor Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication C IPI Pip2-Oaf1 complex GO_0089716 90 YAL053W FLC2 Putative calcium channel involved in calcium release under hypotonic Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication C HDA; IDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 91 YAL054C ACS1 Acetyl-coA synthetase isoform Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions C HDA; IDA mitochondrion; cytosol GO_0005739; GO_0005829 92 YAL055W PEX22 Putative peroxisomal membrane protein Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation C HDA cytoplasm GO_0005737 93 YAL056C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 94 YAL056W GPB2 Multistep regulator of cAMP-PKA signaling Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication C IDA; HDA; HDA plasma membrane; mitochondrion; cytoplasm GO_0005886; GO_0005739; GO_0005737 95 YAL058W CNE1 Calnexin Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast C IDA; HDA integral component of endoplasmic reticulum membrane; endoplasmic reticulum GO_0030176; GO_0005783 96 YAL059C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM1/YAL059W 97 YAL059W ECM1 Pre-ribosomal factor involved in 60S ribosomal protein subunit export Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm C IDA; HDA; IDA preribosome, large subunit precursor; nucleus; nucleolus GO_0030687; GO_0005634; GO_0005730 98 YAL060W BDH1 NAD-dependent (R,R)-butanediol dehydrogenase NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source C HDA cytoplasm GO_0005737 99 YAL061W BDH2 Putative medium-chain alcohol dehydrogenase with similarity to BDH1 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 100 YAL062W GDH3 NADP(+)-dependent glutamate dehydrogenase NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication C HDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 101 YAL063C FLO9 Lectin-like protein with similarity to Flo1p Lectin-like protein with similarity to Flo1p; thought to be expressed and involved in flocculation 102 YAL063C-A YAL063C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 103 YAL064C-A TDA8 Putative protein of unknown function Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene 104 YAL064W YAL064W Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments 105 YAL064W-B YAL064W-B Fungal-specific protein of unknown function Fungal-specific protein of unknown function C HDA endoplasmic reticulum GO_0005783 106 YAL065C YAL065C Putative protein of unknown function Putative protein of unknown function; has homology to FLO1; possible pseudogene 107 YAL066W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 108 YAL067C SEO1 Putative permease Putative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide C HDA cell periphery GO_0071944 109 YAL067W-A YAL067W-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 110 YAL068C PAU8 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions 111 YAL068W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 112 YAL069W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 113 YAR002C-A ERP1 Member of the p24 family involved in ER to Golgi transport Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication C HDA; IDA mitochondrion; ER to Golgi transport vesicle GO_0005739; GO_0030134 114 YAR002W NUP60 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153 C IDA; IDA; IDA; IDA; IDA nuclear pore nuclear basket; cytosol; nuclear pore; nuclear pore central transport channel; nucleus GO_0044615; GO_0005829; GO_0005643; GO_0044613; GO_0005634 115 YAR003W SWD1 Subunit of the COMPASS (Set1C) complex Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 C IPI Set1C/COMPASS complex GO_0048188 116 YAR007C RFA1 Subunit of heterotrimeric Replication Protein A (RPA) Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA; IMP DNA replication factor A complex; nucleus; condensed nuclear chromosome; cytosol; cytoplasm; chromosome, telomeric region GO_0005662; GO_0005634; GO_0000794; GO_0005829; GO_0005737; GO_0000781 117 YAR008W SEN34 Subunit of the tRNA splicing endonuclease Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease C IDA; HDA; HDA tRNA-intron endonuclease complex; mitochondrial outer membrane; mitochondrion GO_0000214; GO_0005741; GO_0005739 118 YAR009C YAR009C Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional; protein product forms cytoplasmic foci upon DNA replication stress C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 119 YAR010C YAR010C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YARCTY1-1 TYB is mutant and probably non-functional 120 YAR014C BUD14 Protein involved in bud-site selection Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; HDA; IDA; HDA; IDA cellular bud tip; incipient cellular bud site; cytoplasm; cellular bud neck; nucleus; Kelch-containing formin regulatory complex GO_0005934; GO_0000131; GO_0005737; GO_0005935; GO_0005634; GO_1990615 121 YAR015W ADE1 N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 122 YAR018C KIN3 Nonessential serine/threonine protein kinase Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol 123 YAR019C CDC15 Protein kinase of the Mitotic Exit Network Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress C IDA; HDA; IDA spindle pole body; cytoplasm; cellular bud neck GO_0005816; GO_0005737; GO_0005935 124 YAR019W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF CDC15/YAR019C 125 YAR020C PAU7 Member of the seripauperin multigene family Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme 126 YAR023C YAR023C Putative integral membrane protein Putative integral membrane protein; member of DUP240 gene family 127 YAR027W UIP3 Putative integral membrane protein of unknown function Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family C IDA; HDA nuclear envelope; fungal-type vacuole membrane GO_0005635; GO_0000329 128 YAR028W YAR028W Putative integral membrane protein Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS C HDA fungal-type vacuole membrane GO_0000329 129 YAR029W YAR029W Member of DUP240 gene family but contains no transmembrane domains Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C IMP cytoplasm GO_0005737 130 YAR030C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 131 YAR031W PRM9 Pheromone-regulated protein Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 132 YAR033W MST28 Putative integral membrane protein, involved in vesicle formation Putative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles; MST28 has a paralog, MST27, that arose from a segmental duplication C IMP; IMP; IPI; IDA endoplasmic reticulum; plasma membrane; Golgi apparatus; integral component of membrane GO_0005783; GO_0005886; GO_0005794; GO_0016021 133 YAR035C-A YAR035C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis 134 YAR035W YAT1 Outer mitochondrial carnitine acetyltransferase Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated C IDA mitochondrion GO_0005739 135 YAR042W SWH1 Protein similar to mammalian oxysterol-binding protein Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction; SWH1 has a paralog, OSH2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA nuclear envelope; early endosome; endoplasmic reticulum; Golgi trans cisterna; nucleus-vacuole junction GO_0005635; GO_0005769; GO_0005783; GO_0000138; GO_0071561 136 YAR047C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 137 YAR050W FLO1 Lectin-like protein involved in flocculation Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; FLO1 has a paralog, FLO5, that arose from a segmental duplication C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 138 YAR053W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 139 YAR060C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YAR060C has a paralog, YHR212C, that arose from a segmental duplication 140 YAR064W YAR064W Pseudogenic fragment with similarity to flocculins Pseudogenic fragment with similarity to flocculins; YAR064W has a paralog, YHR213W-B, that arose from a segmental duplication 141 YAR066W YAR066W Putative GPI protein Putative GPI protein; YAR066W has a paralog, YHR214W, that arose from a segmental duplication 142 YAR068W YAR068W Fungal-specific protein of unknown function Fungal-specific protein of unknown function; induced in respiratory-deficient cells; YAR068W has a paralog, YHR214W-A, that arose from a segmental duplication 143 YAR069C Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; YAR069C has a paralog, YHR214C-D, that arose from a segmental duplication 144 YAR070C Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; YAR070C has a paralog, YHR214C-B, that arose from a segmental duplication 145 YAR071W PHO11 One of three repressible acid phosphatases One of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2; PHO11 has a paralog, PHO12, that arose from a segmental duplication C IDA; HDA extracellular region; cell periphery GO_0005576; GO_0071944 146 YAR073W Nonfunctional protein with homology to IMP dehydrogenase; blocked reading frame, located close to the telomere; not expressed at detectable levels; YAR073W and YAR075W comprise a continuous reading frame in most strains of S. cerevisiae; YAR073W/YAR075W together have a paralog, IMD2, that arose from a segmental duplication 147 YAR075W Non-functional protein with homology IMP dehydrogenase; YAR073W/IMD1 and YAR075W comprise a continuous reading frame in most strains of S. cerevisiae; YAR073W/YAR075W together have a paralog, IMD2, that arose from a segmental duplication 148 YBL001C ECM15 Non-essential protein of unknown function Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis C HDA; HDA; HDA cytoplasm; nucleus; cytosol GO_0005737; GO_0005634; GO_0005829 149 YBL002W HTB2 Histone H2B Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation C IDA replication fork protection complex GO_0031298 150 YBL003C HTA2 Histone H2A Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p C IDA replication fork protection complex GO_0031298 151 YBL004W UTP20 Component of the small-subunit (SSU) processome Component of the small-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA C IDA; IDA; IDA; IDA; IDA; IDA nucleolus; 90S preribosome; nucleoplasm; cytoplasm; preribosome, small subunit precursor; small-subunit processome GO_0005730; GO_0030686; GO_0005654; GO_0005737; GO_0030688; GO_0032040 152 YBL005W PDR3 Transcriptional activator of the pleiotropic drug resistance network Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate; PDR3 has a paralog, PDR1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 153 YBL005W-A YBL005W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 154 YBL005W-B YBL005W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 155 YBL006C LDB7 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions C IDA RSC complex GO_0016586 156 YBL006W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene LDB7/YBL006C 157 YBL007C SLA1 Cytoskeletal protein binding protein Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains C HDA; IDA; IDA; IMP; HDA mating projection tip; actin cortical patch; cell cortex; nucleus; cellular bud neck GO_0043332; GO_0030479; GO_0005938; GO_0005634; GO_0005935 158 YBL008W HIR1 Subunit of the HIR complex Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores C IDA; IPI; IDA HIR complex; nucleus; chromosome, centromeric region GO_0000417; GO_0005634; GO_0000775 159 YBL008W-A YBL008W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 160 YBL009W ALK2 Protein kinase Protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins 161 YBL010C YBL010C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles C HDA clathrin-coated vesicle GO_0030136 162 YBL011W SCT1 Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed C HDA endoplasmic reticulum GO_0005783 163 YBL012C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 164 YBL013W FMT1 Methionyl-tRNA formyltransferase Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate C HDA mitochondrion GO_0005739 165 YBL014C RRN6 Component of the core factor (CF) rDNA transcription factor complex Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p C IDA RNA polymerase I core factor complex GO_0070860 166 YBL015W ACH1 Protein with CoA transferase activity Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth C HDA; IDA mitochondrion; cytosol GO_0005739; GO_0005829 167 YBL016W FUS3 Mitogen-activated serine/threonine protein kinase involved in mating Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis C IDA; IDA; IDA; IDA; HDA cytoplasmic stress granule; cytoplasm; nucleus; mating projection tip; mitochondrion GO_0010494; GO_0005737; GO_0005634; GO_0043332; GO_0005739 168 YBL017C PEP1 Type I transmembrane sorting receptor for multiple vacuolar hydrolases Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments C HDA; IDA fungal-type vacuole; Golgi apparatus GO_0000324; GO_0005794 169 YBL018C POP8 Subunit of both RNase MRP and nuclear RNase P Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA; IDA ribonuclease MRP complex; cytosol; nucleus; nucleolar ribonuclease P complex GO_0000172; GO_0005829; GO_0005634; GO_0005655 170 YBL019W APN2 Class II abasic (AP) endonuclease involved in repair of DNA damage Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII 171 YBL020W RFT1 Membrane protein required for translocation of Man5GlcNac2-PP-Dol Membrane protein required for translocation of Man5GlcNac2-PP-Dol; required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein; essential gene C IGI; HDA endoplasmic reticulum membrane; endoplasmic reticulum GO_0005789; GO_0005783 172 YBL021C HAP3 Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding C IDA CCAAT-binding factor complex GO_0016602 173 YBL022C PIM1 ATP-dependent Lon protease ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria C HDA; IMP mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 174 YBL023C MCM2 Protein involved in DNA replication Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress C IDA; HDA; IDA; IDA; IDA; IDA; IDA cytoplasm; nucleus; nuclear pre-replicative complex; CMG complex; MCM complex; replication fork protection complex; DNA replication preinitiation complex GO_0005737; GO_0005634; GO_0005656; GO_0071162; GO_0042555; GO_0031298; GO_0031261 175 YBL024W NCL1 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 C IDA nucleus GO_0005634 176 YBL025W RRN10 Protein involved in promoting high level transcription of rDNA Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I C IDA; IDA nucleolus; RNA polymerase I upstream activating factor complex GO_0005730; GO_0000500 177 YBL026W LSM2 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C IDA; IDA; HDA; IPI; HDA; IDA; IDA; IDA nucleolus; U6 snRNP; nucleus; small nucleolar ribonucleoprotein complex; cytoplasm; Lsm1-7-Pat1 complex; P-body; U4/U6 x U5 tri-snRNP complex GO_0005730; GO_0005688; GO_0005634; GO_0005732; GO_0005737; GO_1990726; GO_0000932; GO_0046540 178 YBL027W RPL19B Ribosomal 60S subunit protein L19B Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication C HDA; IDA preribosome, large subunit precursor; cytosolic large ribosomal subunit GO_0030687; GO_0022625 179 YBL028C YBL028C Protein of unknown function that may interact with ribosomes Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis C HDA; HDA; HDA; HDA nucleus; preribosome, large subunit precursor; ribosome; nucleolus GO_0005634; GO_0030687; GO_0005840; GO_0005730 180 YBL029C-A YBL029C-A Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica C HDA plasma membrane GO_0005886 181 YBL029W YBL029W Non-essential protein of unknown function Non-essential protein of unknown function C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 182 YBL030C PET9 Major ADP/ATP carrier of the mitochondrial inner membrane Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 183 YBL031W SHE1 Mitotic spindle protein Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function C HDA; IDA; IDA; IDA; IDA microtubule cytoskeleton; astral microtubule; nuclear microtubule; spindle; cellular bud neck GO_0015630; GO_0000235; GO_0005880; GO_0005819; GO_0005935 184 YBL032W HEK2 RNA binding protein involved in asymmetric localization of ASH1 mRNA RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K C IDA; IDA; IDA P-body; nuclear chromosome, telomeric region; cytoplasm GO_0000932; GO_0000784; GO_0005737 185 YBL033C RIB1 GTP cyclohydrolase II GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 186 YBL034C STU1 Component of the mitotic spindle Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles C IDA spindle pole body GO_0005816 187 YBL035C POL12 B subunit of DNA polymerase alpha-primase complex B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation C IDA; IDA; IDA nucleus; alpha DNA polymerase:primase complex; nuclear envelope GO_0005634; GO_0005658; GO_0005635 188 YBL036C YBL036C Putative non-specific single-domain racemase Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS 189 YBL037W APL3 Alpha-adaptin Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport C IDA; IMP cellular bud neck; AP-2 adaptor complex GO_0005935; GO_0030122 190 YBL038W MRPL16 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetically lethal with hac1 mutant suggesting a role in the maturation of secretory proteins C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 191 YBL039C URA7 Major CTP synthase isozyme (see also URA8) Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication C HDA; IDA; HDA cytoplasm; cytoophidium; fungal-type vacuole GO_0005737; GO_0097268; GO_0000324 192 YBL039C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF URA7; identified by expression profiling and mass spectrometry 193 YBL039W-B MIN6 Putative protein of unknown function Putative protein of unknown function C HDA peroxisome GO_0005777 194 YBL040C ERD2 HDEL receptor HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins C IDA integral component of endoplasmic reticulum membrane GO_0030176 195 YBL041W PRE7 Beta 6 subunit of the 20S proteasome Beta 6 subunit of the 20S proteasome C IDA; HDA; HDA proteasome core complex, beta-subunit complex; cytosol; nucleus GO_0019774; GO_0005829; GO_0005634 196 YBL042C FUI1 High affinity uridine permease, localizes to the plasma membrane High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress C IMP; HDA; HDA plasma membrane; fungal-type vacuole; cell periphery GO_0005886; GO_0000324; GO_0071944 197 YBL043W ECM13 Non-essential protein of unknown function Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication 198 YBL044W YBL044W Putative protein of unknown function Putative protein of unknown function; YBL044W is not an essential protein 199 YBL045C COR1 Core subunit of the ubiquinol-cytochrome c reductase complex Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain C IDA; IDA mitochondrial respiratory chain complex III; mitochondrion GO_0005750; GO_0005739 200 YBL046W PSY4 Regulatory subunit of protein phosphatase PP4 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 C HDA; IDA; IDA nucleus; protein phosphatase 4 complex; cytoplasm GO_0005634; GO_0030289; GO_0005737 201 YBL047C EDE1 Scaffold protein involved in the formation of early endocytic sites Putative regulator of cytokinesis; also involved in endocytosis and has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death C IDA; HDA; IDA; HDA; IDA actin cortical patch; mating projection tip; cellular bud tip; cellular bud neck; cytoplasm GO_0030479; GO_0043332; GO_0005934; GO_0005935; GO_0005737 202 YBL048W RRT1 Putative protein of unknown function Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data 203 YBL049W MOH1 Protein of unknown function, essential for stationary phase survival Protein of unknown function; has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase; possibly linked with vacuolar transport 204 YBL050W SEC17 Alpha-SNAP cochaperone Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP C IPI; HDA; IDA; IDA SNARE complex; fungal-type vacuole membrane; cytosol; extrinsic component of membrane GO_0031201; GO_0000329; GO_0005829; GO_0019898 205 YBL051C PIN4 Protein involved in G2/M phase progression and response to DNA damage Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage C HDA cytoplasm GO_0005737 206 YBL052C SAS3 Histone acetyltransferase catalytic subunit of NuA3 complex Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal C IDA; IDA; IDA NuA3b histone acetyltransferase complex; NuA3a histone acetyltransferase complex; NuA3 histone acetyltransferase complex GO_1990468; GO_1990467; GO_0033100 207 YBL053W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 208 YBL054W TOD6 PAC motif binding protein involved in rRNA and ribosome biogenesis PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization C HDA; HDA; HDA Rpd3L-Expanded complex; cytoplasm; nucleus GO_0070210; GO_0005737; GO_0005634 209 YBL055C YBL055C 3'-->5' exonuclease and endonuclease with a possible role in apoptosis 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases C HDA cytoplasm GO_0005737 210 YBL056W PTC3 Type 2C protein phosphatase (PP2C) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 211 YBL057C PTH2 One of two mitochondrially-localized peptidyl-tRNA hydrolases One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 212 YBL058W SHP1 UBX domain-containing substrate adaptor for Cdc48p UBX (ubiquitin regulatory X) domain-containing protein; regulates Glc7p phosphatase activity; shp1 mutants are impaired in growth and mitotic progression; functions in growth and mitotic progression require Cdc48p binding; mitotic phenotype is caused by reduced Glc7p activity; interacts with ubiquitylated proteins, required for degradation of a ubiquitylated model substrate C HDA; HDA; HDA cytosol; nucleus; cytoplasm GO_0005829; GO_0005634; GO_0005737 213 YBL059C-A CMC2 Protein involved in respiratory chain complex assembly or maintenance Protein involved in respiratory chain complex assembly or maintenance; protein of the mitochondrial intermembrane space; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold C HDA; HDA; HDA; IDA mitochondrion; nucleus; cytoplasm; mitochondrial intermembrane space GO_0005739; GO_0005634; GO_0005737; GO_0005758 214 YBL059W IAI11 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 215 YBL060W YEL1 Guanine nucleotide exchange factor specific for Arf3p Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip C HDA; IDA; IDA cytoplasm; cellular bud neck; cellular bud tip GO_0005737; GO_0005935; GO_0005934 216 YBL061C SKT5 Activator of Chs3p (chitin synthase III) during vegetative growth Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication C IDA; IDA; IDA cellular bud neck; cellular bud neck septin ring; incipient cellular bud site GO_0005935; GO_0000144; GO_0000131 217 YBL062W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 218 YBL063W KIP1 Kinesin-related motor protein Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions C IDA; IDA; HDA spindle pole body; spindle microtubule; nucleus GO_0005816; GO_0005876; GO_0005634 219 YBL064C PRX1 Mitochondrial peroxiredoxin with thioredoxin peroxidase activity Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 220 YBL065W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene 221 YBL066C SEF1 Putative transcription factor Putative transcription factor; has homolog in Kluyveromyces lactis 222 YBL067C UBP13 Ubiquitin-specific protease that cleaves Ub-protein fusions Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication 223 YBL068W PRS4 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic C HDA; IDA cytoplasm; ribose phosphate diphosphokinase complex GO_0005737; GO_0002189 224 YBL068W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by fungal homology and RT-PCR 225 YBL069W AST1 Lipid raft associated protein Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA late endosome; plasma membrane; Golgi apparatus; membrane raft GO_0005770; GO_0005886; GO_0005794; GO_0045121 226 YBL070C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 227 YBL071C YBL071C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 228 YBL071C-B YBL071C-B Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 229 YBL071W-A KTI11 Zn-ribbon protein that co-purifies with Dph1 and Dph2 Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 230 YBL072C RPS8A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication C HDA; IDA 90S preribosome; cytosolic small ribosomal subunit GO_0030686; GO_0022627 231 YBL073W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C 232 YBL074C AAR2 Component of the U5 snRNP complex Component of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron C IDA U5 snRNP GO_0005682 233 YBL075C SSA3 ATPase involved in protein folding and the response to stress ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication C IDA cytosol GO_0005829 234 YBL076C ILS1 Cytoplasmic isoleucine-tRNA synthetase Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A C IDA cytosol GO_0005829 235 YBL077W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C 236 YBL078C ATG8 Component of autophagosomes and Cvt vesicles Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis C IDA; IDA; IDA; IPI; IDA; IDA autophagosome; cytosol; NA; fungal-type vacuole membrane; pre-autophagosomal structure; extrinsic component of membrane GO_0005776; GO_0005829; GO_0120095; GO_0000329; GO_0000407; GO_0019898 237 YBL079W NUP170 Subunit of inner ring of nuclear pore complex (NPC) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication C IDA; IDA nuclear pore inner ring; nuclear pore GO_0044611; GO_0005643 238 YBL080C PET112 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog C IGI; IDA mitochondrion; glutamyl-tRNA(Gln) amidotransferase complex GO_0005739; GO_0030956 239 YBL081W YBL081W Non-essential protein of unknown function Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport 240 YBL082C ALG3 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins C IMP endoplasmic reticulum GO_0005783 241 YBL083C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 242 YBL084C CDC27 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition C IDA; IDA nuclear periphery; anaphase-promoting complex GO_0034399; GO_0005680 243 YBL085W BOI1 Protein implicated in polar growth Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication C HDA; HDA; IDA; IDA; HDA; HDA mating projection tip; cellular bud neck; site of polarized growth; cellular bud; cytoplasm; plasma membrane GO_0043332; GO_0005935; GO_0030427; GO_0005933; GO_0005737; GO_0005886 244 YBL086C YBL086C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 245 YBL087C RPL23A Ribosomal 60S subunit protein L23A Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 246 YBL088C TEL1 Protein kinase primarily involved in telomere length regulation Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) C HDA mitochondrion GO_0005739 247 YBL089W AVT5 Putative transporter Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication 248 YBL090W MRP21 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 249 YBL091C MAP2 Methionine aminopeptidase Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 250 YBL091C-A SCS22 Protein involved in regulation of phospholipid metabolism Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication C HDA cytosol GO_0005829 251 YBL092W RPL32 Ribosomal 60S subunit protein L32 Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog C IDA cytosolic large ribosomal subunit GO_0022625 252 YBL093C ROX3 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme C IDA; IDA core mediator complex; nucleus GO_0070847; GO_0005634 253 YBL094C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W 254 YBL095W MRX3 Protein that associates with mitochondrial ribosome Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 255 YBL096C Non-essential protein of unknown function 256 YBL097W BRN1 Subunit of the condensin complex Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission C IPI; IDA nuclear condensin complex; nucleus GO_0000799; GO_0005634 257 YBL098W BNA4 Kynurenine 3-monooxygenase Kynurenine 3-mono oxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 258 YBL099W ATP1 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo C IDA; IDA; IDA mitochondrial proton-transporting ATP synthase, catalytic core; mitochondrion; mitochondrial nucleoid GO_0005754; GO_0005739; GO_0042645 259 YBL100C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1 260 YBL100W-A YBL100W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 261 YBL100W-B YBL100W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 262 YBL100W-C YBL100W-C Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 263 YBL101C ECM21 Protein involved in regulating endocytosis of plasma membrane proteins Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication C HDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 264 YBL102W SFT2 Tetra-spanning membrane protein found mostly in the late Golgi Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 C IDA; IDA Golgi trans cisterna; Golgi membrane GO_0000138; GO_0000139 265 YBL103C RTG3 bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 266 YBL104C SEA4 Subunit of SEACAT, a subcomplex of the SEA complex Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites C IDA; IDA; HDA Seh1-associated complex; extrinsic component of fungal-type vacuolar membrane; cytoplasm GO_0035859; GO_0097042; GO_0005737 267 YBL105C PKC1 Protein serine/threonine kinase Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) C HDA; IDA; IDA; IDA; HDA; HDA; IDA prospore septin filament array; nucleus; cytoskeleton; cytoplasm; plasma membrane; cellular bud neck; site of polarized growth GO_0032165; GO_0005634; GO_0005856; GO_0005737; GO_0005886; GO_0005935; GO_0030427 268 YBL106C SRO77 Protein with roles in exocytosis and cation homeostasis Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication C IPI; HDA plasma membrane; cellular bud neck GO_0005886; GO_0005935 269 YBL107C MIX23 Mitochondrial intermembrane space protein of unknown function Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) C IDA; HDA; HDA mitochondrial intermembrane space; mitochondrion; cytoplasm GO_0005758; GO_0005739; GO_0005737 270 YBL107W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the Ty2 LTR YBLWdelta2; YBL107W-A has a paralog, YER138W-A, that arose from a single-locus duplication 271 YBL108C-A PAU9 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions 272 YBL108W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 273 YBL109W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 274 YBL111C YBL111C Helicase-like protein encoded within the telomeric Y' element Helicase-like protein encoded within the telomeric Y' element; relocalizes from mitochondrion to cytoplasm upon DNA replication stress C HDA mitochondrion GO_0005739 275 YBL112C YBL112C Putative protein of unknown function Putative protein of unknown function; YBL112C is contained within TEL02L 276 YBL113C YBL113C Helicase-like protein encoded within the telomeric Y' element Helicase-like protein encoded within the telomeric Y' element 277 YBL113W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 278 YBR001C NTH2 Putative neutral trehalase, required for thermotolerance Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 279 YBR002C RER2 Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1 Cis-prenyltransferase involved in dolichol synthesis; major enzyme of polyprenol synthesis in both the endoplasmic reticulum (ER) and in lipid droplets; participates in ER protein sorting C IDA; IDA; IDA endoplasmic reticulum; dehydrodolichyl diphosphate synthase complex; lipid particle GO_0005783; GO_1904423; GO_0005811 280 YBR003W COQ1 Hexaprenyl pyrophosphate synthetase Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis C HDA mitochondrion GO_0005739 281 YBR004C GPI18 Functional ortholog of human PIG-V Functional ortholog of human PIG-V; PIG-V is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria C IPI; IDA; HDA; IDA mannosyltransferase complex; endoplasmic reticulum; mitochondrion; integral component of endoplasmic reticulum membrane GO_0031501; GO_0005783; GO_0005739; GO_0030176 282 YBR005W RCR1 Protein of the ER membrane involved in cell wall chitin deposition Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication C IDA integral component of endoplasmic reticulum membrane GO_0030176 283 YBR006W UGA2 Succinate semialdehyde dehydrogenase Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm C HDA cytoplasm GO_0005737 284 YBR007C DSF2 Deletion suppressor of mpt5 mutation Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA cellular bud tip GO_0005934 285 YBR008C FLR1 Plasma membrane transporter of the major facilitator superfamily Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress C HDA; HDA; HDA; IDA cell periphery; cytoplasm; nucleus; plasma membrane GO_0071944; GO_0005737; GO_0005634; GO_0005886 286 YBR009C HHF1 Histone H4 Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity C IDA replication fork protection complex GO_0031298 287 YBR010W HHT1 Histone H3 Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage C HDA; IDA; IDA nucleus; CENP-A containing nucleosome; replication fork protection complex GO_0005634; GO_0043505; GO_0031298 288 YBR011C IPP1 Cytoplasmic inorganic pyrophosphatase (PPase) Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 289 YBR012C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by iron-regulated transcriptional activator Aft2p 290 YBR012W-A YBR012W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 291 YBR012W-B YBR012W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 292 YBR013C YBR013C Putative protein of unknown function Putative protein of unknown function; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein C HDA endoplasmic reticulum GO_0005783 293 YBR014C GRX7 Cis-golgi localized monothiol glutaredoxin Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; IDA Golgi lumen; Golgi apparatus; fungal-type vacuole; integral component of membrane; cis-Golgi network GO_0005796; GO_0005794; GO_0000324; GO_0016021; GO_0005801 294 YBR015C MNN2 Alpha-1,2-mannosyltransferase Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment C IDA Golgi apparatus GO_0005794 295 YBR016W YBR016W Tail-anchored plasma membrane protein with a conserved CYSTM module Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication C IDA; IDA; IDA plasma membrane; mating projection membrane; cellular bud membrane GO_0005886; GO_0070250; GO_0033101 296 YBR017C KAP104 Transportin or cytosolic karyopherin beta 2 Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis C IDA; IDA; IDA cytosol; cellular bud neck; cellular bud tip GO_0005829; GO_0005935; GO_0005934 297 YBR018C GAL7 Galactose-1-phosphate uridyl transferase Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism C IDA cytoplasm GO_0005737 298 YBR019C GAL10 UDP-glucose-4-epimerase UDP-glucose-4-epimerase; catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers C IDA cytosol GO_0005829 299 YBR020W GAL1 Galactokinase Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication C IGI cytoplasm GO_0005737 300 YBR021W FUR4 Plasma membrane localized uracil permease Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation C IDA; HDA; IDA; HDA membrane raft; fungal-type vacuole; plasma membrane; cell periphery GO_0045121; GO_0000324; GO_0005886; GO_0071944 301 YBR022W POA1 Phosphatase that is highly specific for ADP-ribose 1''-phosphate Phosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing 302 YBR023C CHS3 Chitin synthase III Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention C IDA; HDA; IDA; HDA; IDA; IDA; IDA cytoplasm; endoplasmic reticulum; incipient cellular bud site; mating projection tip; cellular bud neck; prospore membrane; chitosome GO_0005737; GO_0005783; GO_0000131; GO_0043332; GO_0005935; GO_0005628; GO_0045009 303 YBR024W SCO2 Protein anchored to mitochondrial inner membrane Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial envelope GO_0005739; GO_0005740 304 YBR025C OLA1 P-loop ATPase with similarity to human OLA1 and bacterial YchF P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress C IDA cytoplasm GO_0005737 305 YBR026C ETR1 2-enoyl thioester reductase 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis C IDA mitochondrion GO_0005739 306 YBR027C YBR027C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 307 YBR028C YPK3 AGC kinase AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner C HDA cytoplasm GO_0005737 308 YBR029C CDS1 Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase) Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids C IDA; IDA endoplasmic reticulum; Golgi to plasma membrane transport vesicle GO_0005783; GO_0070319 309 YBR030W RKM3 Ribosomal lysine methyltransferase Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia C HDA; IDA nucleus; cytosol GO_0005634; GO_0005829 310 YBR031W RPL4A Ribosomal 60S subunit protein L4A Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 311 YBR032W YBR032W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 312 YBR033W EDS1 Putative zinc cluster protein, predicted to be a transcription factor Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication 313 YBR034C HMT1 Nuclear SAM-dependent mono- and asymmetric methyltransferase Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. C IDA nucleus GO_0005634 314 YBR035C PDX3 Pyridoxine (pyridoxamine) phosphate oxidase Pyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism C IDA mitochondrial intermembrane space GO_0005758 315 YBR036C CSG2 Endoplasmic reticulum membrane protein Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane; fungal-type vacuole GO_0005783; GO_0030176; GO_0000324 316 YBR037C SCO1 Copper-binding protein of mitochondrial inner membrane Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 317 YBR038W CHS2 Chitin synthase II Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis C IDA cellular bud neck GO_0005935 318 YBR039W ATP3 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis C HDA; IDA mitochondrion; mitochondrial proton-transporting ATP synthase, central stalk GO_0005739; GO_0005756 319 YBR040W FIG1 Integral membrane protein required for efficient mating Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating C HDA; IDA; IDA fungal-type vacuole; fungal-type cell wall; mating projection tip GO_0000324; GO_0009277; GO_0043332 320 YBR041W FAT1 Very long chain fatty acyl-CoA synthetase and fatty acid transporter Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids C IDA; IDA; IMP; HDA; HDA; IDA lipid particle; intrinsic component of the cytoplasmic side of the plasma membrane; peroxisome; cell periphery; fungal-type vacuole membrane; endoplasmic reticulum GO_0005811; GO_0031235; GO_0005777; GO_0071944; GO_0000329; GO_0005783 321 YBR042C CST26 Acyltransferase Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication C HDA lipid particle GO_0005811 322 YBR043C QDR3 Multidrug transporter of the major facilitator superfamily Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 323 YBR044C TCM62 Protein involved in assembly of the succinate dehydrogenase complex Protein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 324 YBR045C GIP1 Meiosis-specific regulatory subunit of the Glc7p protein phosphatase Meiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p C IDA; IPI prospore membrane; protein phosphatase type 1 complex GO_0005628; GO_0000164 325 YBR046C ZTA1 NADPH-dependent quinone reductase NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 326 YBR047W FMP23 Putative protein of unknown function Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 327 YBR048W RPS11B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication C IDA; HDA cytosolic small ribosomal subunit; 90S preribosome GO_0022627; GO_0030686 328 YBR049C REB1 RNA polymerase I enhancer binding protein RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication C IDA nucleoplasm GO_0005654 329 YBR050C REG2 Regulatory subunit of the Glc7p type-1 protein phosphatase Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication C IPI protein phosphatase type 1 complex GO_0000164 330 YBR051W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase 331 YBR052C RFS1 Protein of unknown function Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication C HDA; IDA cytoplasm; membrane raft GO_0005737; GO_0045121 332 YBR053C YBR053C Putative protein of unknown function Putative protein of unknown function; induced by cell wall perturbation 333 YBR054W YRO2 Protein with a putative role in response to acid stress Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication C IDA; HDA; HDA; HDA plasma membrane; endoplasmic reticulum; mitochondrion; cellular bud GO_0005886; GO_0005783; GO_0005739; GO_0005933 334 YBR055C PRP6 Splicing factor Splicing factor; component of the U4/U6-U5 snRNP complex C IDA; IDA U4/U6 x U5 tri-snRNP complex; U4/U6 snRNP GO_0046540; GO_0071001 335 YBR056C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the dubious ORF YBR056W-A 336 YBR056W YBR056W Putative glycoside hydrolase of the mitochondrial intermembrane space Putative glycoside hydrolase of the mitochondrial intermembrane space C HDA; IDA; HDA cytoplasm; mitochondrial intermembrane space; cytosol GO_0005737; GO_0005758; GO_0005829 337 YBR056W-A MNC1 Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps dubious ORF YBR056C-B; YBR056W-A has a paralog, YDR034W-B, that arose from the whole genome duplication 338 YBR057C MUM2 Protein essential for meiotic DNA replication and sporulation Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex C IDA; HDA MIS complex; cytoplasm GO_0036396; GO_0005737 339 YBR058C UBP14 Ubiquitin-specific protease Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T 340 YBR058C-A TSC3 Protein that stimulates the activity of serine palmitoyltransferase Protein that stimulates the activity of serine palmitoyltransferase; involved in sphingolipid biosynthesis; Lcb1p and Lcb2p are the two components of serine palmitoyltransferase C HDA; IDA endoplasmic reticulum; SPOTS complex GO_0005783; GO_0035339 341 YBR059C AKL1 Ser-Thr protein kinase Ser-Thr protein kinase; member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization C HDA; HDA; HDA; HDA cell periphery; cellular bud neck; cytoplasm; prospore membrane GO_0071944; GO_0005935; GO_0005737; GO_0005628 342 YBR060C ORC2 Subunit of the origin recognition complex (ORC) Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p C IMP; IDA; IDA nuclear origin of replication recognition complex; DNA replication preinitiation complex; nuclear pre-replicative complex GO_0005664; GO_0031261; GO_0005656 343 YBR061C TRM7 2'-O-ribose methyltransferase 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively C IDA cytoplasm GO_0005737 344 YBR062C YBR062C Protein of unknown function that interacts with Msb2p Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway 345 YBR063C YBR063C Putative protein of unknown function Putative protein of unknown function; YBR063C is not an essential gene 346 YBR064W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR063C 347 YBR065C ECM2 Pre-mRNA splicing factor Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p C HDA nucleus GO_0005634 348 YBR066C NRG2 Transcriptional repressor Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose C IDA nucleus GO_0005634 349 YBR067C TIP1 Major cell wall mannoprotein with possible lipase activity Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins C IDA; HDA; HDA fungal-type cell wall; cytosol; cell periphery GO_0009277; GO_0005829; GO_0071944 350 YBR068C BAP2 High-affinity leucine permease High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 351 YBR069C TAT1 Amino acid transporter for valine, leucine, isoleucine, and tyrosine Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress C IDA; HDA; HDA plasma membrane; endoplasmic reticulum; cell periphery GO_0005886; GO_0005783; GO_0071944 352 YBR070C ALG14 Component of UDP-GlcNAc transferase Component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases C IDA; IDA; IDA; IPI integral component of endoplasmic reticulum membrane; intrinsic component of endoplasmic reticulum membrane; nuclear outer membrane-endoplasmic reticulum membrane network; UDP-N-acetylglucosamine transferase complex GO_0030176; GO_0031227; GO_0042175; GO_0043541 353 YBR071W YBR071W Protein of unknown function found in the cytoplasm and bud neck Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking C IDA; IDA cellular bud neck; cytoplasm GO_0005935; GO_0005737 354 YBR072C-A YBR072C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 355 YBR072W HSP26 Small heat shock protein (sHSP) with chaperone activity Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity C IDA; HDA; IDA; HDA cytoplasmic stress granule; cytoplasm; nucleus; mitochondrion GO_0010494; GO_0005737; GO_0005634; GO_0005739 356 YBR073W RDH54 DNA-dependent ATPase DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p 357 YBR074W PFF1 Multi-spanning vacuolar membrane protease Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to the M28 family of metalloproteases; has a lumenal-facing protease domain; proposed role in vacuole physiology C IDA fungal-type vacuole membrane GO_0000329 358 YBR076C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM8; identified by fungal homology and RT-PCR 359 YBR076W ECM8 Non-essential protein of unknown function Non-essential protein of unknown function 360 YBR077C SLM4 Subunit of the EGO/GSE complex Component of the EGO and GSE complexes; essential for integrity and function of EGO; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 C HDA; HDA; IPI; IDA cytoplasm; fungal-type vacuole membrane; EGO complex; late endosome membrane GO_0005737; GO_0000329; GO_0034448; GO_0031902 361 YBR078W ECM33 GPI-anchored protein of unknown function GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication C IDA; IDA; HDA; HDA plasma membrane; fungal-type cell wall; mitochondrion; endoplasmic reticulum GO_0005886; GO_0009277; GO_0005739; GO_0005783 362 YBR079C RPG1 eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3) eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation C IDA; IDA; IDA; HDA; IDA multi-eIF complex; incipient cellular bud site; eukaryotic translation initiation factor 3 complex; cytoplasm; cytoplasmic stress granule GO_0043614; GO_0000131; GO_0005852; GO_0005737; GO_0010494 363 YBR080C SEC18 AAA ATPase and SNARE disassembly chaperone AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF) C HDA; HDA; IDA cytosol; mating projection tip; Golgi apparatus GO_0005829; GO_0043332; GO_0005794 364 YBR081C SPT7 Subunit of the SAGA transcriptional regulatory complex Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex C HDA; IDA; IDA mitochondrion; SLIK (SAGA-like) complex; SAGA complex GO_0005739; GO_0046695; GO_0000124 365 YBR082C UBC4 Ubiquitin-conjugating enzyme (E2) Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication C IPI proteasome complex GO_0000502 366 YBR083W TEC1 Transcription factor targeting filamentation genes and Ty1 expression Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member C IDA; IDA nucleus; Tec1p-Ste12p-Dig1p complex GO_0005634; GO_1990527 367 YBR084C-A RPL19A Ribosomal 60S subunit protein L19A Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 368 YBR084W MIS1 Mitochondrial C1-tetrahydrofolate synthase Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase C IDA mitochondrion GO_0005739 369 YBR085C-A YBR085C-A Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 370 YBR085W AAC3 Mitochondrial inner membrane ADP/ATP translocator Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 371 YBR086C IST2 Cortical ER protein involved in ER-plasma membrane tethering Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process C IDA; IDA; HDA; IDA cellular bud membrane; plasma membrane; cell periphery; cortical endoplasmic reticulum GO_0033101; GO_0005886; GO_0071944; GO_0032541 372 YBR087W RFC5 Subunit of heteropentameric Replication factor C (RF-C) Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon C IMP; IDA; IPI; IPI DNA replication factor C complex; Rad17 RFC-like complex; Elg1 RFC-like complex; Ctf18 RFC-like complex GO_0005663; GO_0031389; GO_0031391; GO_0031390 373 YBR088C POL30 Proliferating cell nuclear antigen (PCNA) Proliferating cell nuclear antigen (PCNA); functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair C IPI; IDA; IDA PCNA complex; nucleus; replication fork GO_0043626; GO_0005634; GO_0005657 374 YBR089C-A NHP6B High-mobility group (HMG) protein High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication C IDA nucleus GO_0005634 375 YBR089W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 376 YBR090C YBR090C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 377 YBR091C TIM12 Essential protein of the inner mitochondrial membrane Essential protein of the inner mitochondrial membrane; peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins C IDA; HDA mitochondrial inner membrane protein insertion complex; mitochondrion GO_0042721; GO_0005739 378 YBR092C PHO3 Constitutively expressed acid phosphatase similar to Pho5p Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin C IMP; HDA cell wall-bounded periplasmic space; endoplasmic reticulum GO_0030287; GO_0005783 379 YBR093C PHO5 Repressible acid phosphatase Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 380 YBR094W PBY1 Putative tubulin tyrosine ligase associated with P-bodies Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress C HDA; IDA cytoplasm; P-body GO_0005737; GO_0000932 381 YBR095C RXT2 Component of the histone deacetylase Rpd3L complex Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia C IDA; IDA; HDA; HDA Rpd3L complex; cytosol; Rpd3L-Expanded complex; nucleus GO_0033698; GO_0005829; GO_0070210; GO_0005634 382 YBR096W YBR096W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER C HDA endoplasmic reticulum GO_0005783 383 YBR097W VPS15 Serine/threonine protein kinase involved in vacuolar protein sorting Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery C IDA; IDA; IDA; IDA; HDA; IDA; HDA phosphatidylinositol 3-kinase complex, class III, type I; phosphatidylinositol 3-kinase complex, class III, type II; nucleus-vacuole junction; NA; mitochondrion; nuclear pore; fungal-type vacuole membrane GO_0034271; GO_0034272; GO_0071561; GO_0120095; GO_0005739; GO_0005643; GO_0000329 384 YBR098W MMS4 Subunit of structure-specific Mms4p-Mus81p endonuclease Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p C IDA Holliday junction resolvase complex GO_0048476 385 YBR099C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 386 YBR101C FES1 Hsp70 (Ssa1p) nucleotide exchange factor Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress C IDA cytosol GO_0005829 387 YBR102C EXO84 Exocyst subunit with dual roles in exocytosis and spliceosome assembly Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate C HDA; IDA; IDA; IDA; IDA; HDA mating projection tip; cellular bud neck; exocyst; cellular bud tip; incipient cellular bud site; prospore membrane GO_0043332; GO_0005935; GO_0000145; GO_0005934; GO_0000131; GO_0005628 388 YBR103C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by homology to related yeast 389 YBR103W SIF2 WD40 repeat-containing subunit of Set3C histone deacetylase complex WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus C IDA; IDA; HDA Set3 complex; nucleus; Rpd3L-Expanded complex GO_0034967; GO_0005634; GO_0070210 390 YBR104W YMC2 Putative mitochondrial inner membrane transporter Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 391 YBR105C VID24 GID Complex regulatory subunit GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles C IDA; IDA; IDA; HDA cytoplasmic vesicle; GID complex; extrinsic component of membrane; cytosol GO_0031410; GO_0034657; GO_0019898; GO_0005829 392 YBR106W SND3 Protein involved in SRP-independent targeting of substrates to the ER Probable membrane protein; involved in phosphate transport; role in the maturation of secretory proteins; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations C IDA; HDA; HDA membrane; mitochondrion; endoplasmic reticulum GO_0016020; GO_0005739; GO_0005783 393 YBR107C IML3 Outer kinetochore protein and component of the Ctf19 complex Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 C IDA condensed nuclear chromosome outer kinetochore GO_0000942 394 YBR108W AIM3 Protein that inhibits barbed-end actin filament elongation Protein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss C IDA; IDA membrane raft; actin cortical patch GO_0045121; GO_0030479 395 YBR109C CMD1 Calmodulin Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin C IDA; HDA; IDA; HDA; IDA; IDA central plaque of spindle pole body; cellular bud neck; cellular bud tip; cellular bud; mating projection tip; incipient cellular bud site GO_0005823; GO_0005935; GO_0005934; GO_0005933; GO_0043332; GO_0000131 396 YBR109W-A Putative protein of unknown function; questionable ORF from MIPS 397 YBR110W ALG1 Mannosyltransferase Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog C IPI endoplasmic reticulum GO_0005783 398 YBR111C YSA1 Nudix hydrolase family member with ADP-ribose pyrophosphatase activity Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate C HDA; HDA; HDA mitochondrion; nucleus; cytoplasm GO_0005739; GO_0005634; GO_0005737 399 YBR111W-A SUS1 Component of both the SAGA histone acetylase and TREX-2 complexes Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP C IDA; IDA; IDA; IDA; IDA DUBm complex; nuclear pore; nucleoplasm; transcription export complex 2; SAGA complex GO_0071819; GO_0005643; GO_0005654; GO_0070390; GO_0000124 400 YBR112C CYC8 General transcriptional co-repressor General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+] C IDA; IPI nucleus; transcriptional repressor complex GO_0005634; GO_0017053 401 YBR113W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 402 YBR114W RAD16 Nucleotide excision repair (NER) protein Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex C IDA; IDA Cul3-RING ubiquitin ligase complex; nucleotide-excision repair factor 4 complex GO_0031463; GO_0000113 403 YBR115C LYS2 Alpha aminoadipate reductase Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p C HDA cytoplasm GO_0005737 404 YBR116C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 405 YBR117C TKL2 Transketolase Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 406 YBR118W TEF2 Translational elongation factor EF-1 alpha Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; TEF2-RFP levels increase during replicative aging; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication C IPI; IDA; IDA mitochondrion; cytoplasm; fungal-type vacuole membrane GO_0005739; GO_0005737; GO_0000329 407 YBR119W MUD1 U1 snRNP A protein U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing C IDA; IDA U2-type prespliceosome; U1 snRNP GO_0071004; GO_0005685 408 YBR120C CBP6 Mitochondrial protein required for translation of the COB mRNA Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation C IPI; HDA; IDA Cbp3p-Cbp6 complex; mitochondrion; mitochondrial ribosome GO_0061671; GO_0005739; GO_0005761 409 YBR121C GRS1 Cytoplasmic and mitochondrial glycyl-tRNA synthase Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication C IMP; IMP mitochondrion; cytoplasm GO_0005739; GO_0005737 410 YBR121C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely contained within the verified gene GRS1; identified by expression profiling and mass spectrometry 411 YBR122C MRPL36 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 412 YBR123C TFC1 Subunit of RNA polymerase III transcription initiation factor complex Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 C IDA transcription factor TFIIIC complex GO_0000127 413 YBR124W Putative protein of unknown function 414 YBR125C PTC4 Cytoplasmic type 2C protein phosphatase (PP2C) Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity C HDA cytoplasm GO_0005737 415 YBR126C TPS1 Synthase subunit of trehalose-6-P synthase/phosphatase complex Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid C IMP; HDA alpha,alpha-trehalose-phosphate synthase complex (UDP-forming); cytoplasm GO_0005946; GO_0005737 416 YBR126W-A MEO1 Putative protein of unknown function Protein of unknown function; identified by gene-trapping, microarray analysis, and genome-wide homology searches; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR126W-B C HDA endoplasmic reticulum GO_0005783 417 YBR126W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YBR126W-A; identified by SAGE 418 YBR127C VMA2 Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; HDA; IDA fungal-type vacuole membrane; vacuolar proton-transporting V-type ATPase, V1 domain; vacuole-mitochondrion membrane contact site; cytoplasm; cytoplasmic stress granule GO_0000329; GO_0000221; GO_1990816; GO_0005737; GO_0010494 419 YBR128C ATG14 Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p C IDA; IDA; IDA; IDA; IDA phosphatidylinositol 3-kinase complex, class III, type I; pre-autophagosomal structure; fungal-type vacuole membrane; NA; extrinsic component of membrane GO_0034271; GO_0000407; GO_0000329; GO_0120095; GO_0019898 420 YBR129C OPY1 Protein of unknown function Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; HDA; IDA plasma membrane; mitochondrion; cytoplasm GO_0005886; GO_0005739; GO_0005737 421 YBR130C SHE3 Protein adaptor between Myo4p and the She2p-mRNA complex Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance C IDA; HDA cellular bud tip; cytoplasm GO_0005934; GO_0005737 422 YBR131C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR131W; identified by gene-trapping, microarray analysis, and genome-wide homology searches 423 YBR131W CCZ1 Subunit of a heterodimeric guanine nucleotide exchange factor (GEF) Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy C HDA; IDA; IDA; IDA cytosol; Mon1-Ccz1 complex; endosome; late endosome GO_0005829; GO_0035658; GO_0005768; GO_0005770 424 YBR132C AGP2 Plasma membrane regulator of polyamine and carnitine transport Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes C IDA; HDA; IDA; IDA endoplasmic reticulum membrane; endoplasmic reticulum; integral component of plasma membrane; fungal-type vacuole membrane GO_0005789; GO_0005783; GO_0005887; GO_0000329 425 YBR133C HSL7 Protein arginine N-methyltransferase Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress C IMP; HDA; IDA; HDA; IDA cellular bud neck septin ring; cytoplasm; outer plaque of spindle pole body; cellular bud neck; cellular bud neck septin collar GO_0000144; GO_0005737; GO_0005824; GO_0005935; GO_0032174 426 YBR134W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 427 YBR135W CKS1 Cyclin-dependent protein kinase regulatory subunit and adaptor Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p(Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates the phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters C HDA; IDA; HDA nucleus; cyclin-dependent protein kinase holoenzyme complex; cytoplasm GO_0005634; GO_0000307; GO_0005737 428 YBR136W MEC1 Genome integrity checkpoint protein and PI kinase superfamily member Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres C HDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 429 YBR137W YBR137W Protein with a role in ER delivery of tail-anchored membrane proteins Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene C IDA; IDA TRC complex; cytoplasm GO_0072380; GO_0005737 430 YBR138C YBR138C Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene C HDA cytoplasm GO_0005737 431 YBR139W ATG42 Vacuolar serine-type carboxypeptidase Putative serine type carboxypeptidase; role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner C HDA; IDA fungal-type vacuole; fungal-type vacuole lumen GO_0000324; GO_0000328 432 YBR140C IRA1 GTPase-activating protein GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication C HDA; IMP mitochondrion; membrane GO_0005739; GO_0016020 433 YBR141C BMT2 Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene C IDA nucleolus GO_0005730 434 YBR141W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YBR141C; identified by gene-trapping, microarray analysis, and genome-wide homology searching 435 YBR142W MAK5 Essential nucleolar protein Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits C IDA nucleolus GO_0005730 436 YBR143C SUP45 Polypeptide release factor (eRF1) in translation termination Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p C IDA; IDA translation release factor complex; cytoplasmic stress granule GO_0018444; GO_0010494 437 YBR144C YBR144C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene 438 YBR145W ADH5 Alcohol dehydrogenase isoenzyme V Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 439 YBR146W MRPS9 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 440 YBR147W RTC2 Putative vacuolar membrane transporter for cationic amino acids Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication C HDA; IDA mitochondrion; integral component of fungal-type vacuolar membrane GO_0005739; GO_0071627 441 YBR148W YSW1 Protein required for normal prospore membrane formation Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication C IDA; HDA prospore membrane; septin complex GO_0005628; GO_0031105 442 YBR149W ARA1 NADP+ dependent arabinose dehydrogenase NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product C IDA cytosol GO_0005829 443 YBR150C TBS1 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication C HDA; HDA; HDA nucleus; cytoplasm; mitochondrion GO_0005634; GO_0005737; GO_0005739 444 YBR151W APD1 Protein of unknown function Protein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 445 YBR152W SPP381 mRNA splicing factor, component of U4/U6.U5 tri-snRNP mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; U4/U6 x U5 tri-snRNP complex; nucleus GO_0005829; GO_0046540; GO_0005634 446 YBR153W RIB7 Diaminohydroxyphoshoribosylaminopyrimidine deaminase Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway 447 YBR154C RPB5 RNA polymerase subunit ABC27 RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation C IDA; IDA; IDA DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase III complex GO_0005665; GO_0005736; GO_0005666 448 YBR155W CNS1 TPR-containing co-chaperone TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion C IPI cytoplasm GO_0005737 449 YBR156C SLI15 Subunit of the conserved chromosomal passenger complex (CPC) Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p C IDA; IDA; IDA; IDA chromosome passenger complex; kinetochore microtubule; spindle microtubule; spindle midzone GO_0032133; GO_0005828; GO_0005876; GO_0051233 450 YBR157C ICS2 Protein of unknown function Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization 451 YBR158W AMN1 Protein required for daughter cell separation Protein required for daughter cell separation; multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) C IDA; IDA; IDA cellular bud; nucleus; cytoplasm GO_0005933; GO_0005634; GO_0005737 452 YBR159W IFA38 Microsomal beta-keto-reductase Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides C IDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 453 YBR160W CDC28 Cyclin-dependent kinase (CDK) catalytic subunit Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1 (CLNs), S and G2/M (CLBs) phase cyclins, which provide substrate specificity; regulates cell cycle and basal transcription, chromosome duplication and segregation, lipid biosynthesis, membrane trafficking, polarized growth, and morphogenesis; abundance increases in DNA replication stress; transcript induction in osmostress involves antisense RNA C IDA; HDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA cytoplasmic stress granule; ribosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; cellular bud neck; nucleus; endoplasmic reticulum; astral microtubule; spindle pole body GO_0010494; GO_0005840; GO_0005737; GO_0000307; GO_0005935; GO_0005634; GO_0005783; GO_0000235; GO_0005816 454 YBR161W CSH1 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 455 YBR162C TOS1 Covalently-bound cell wall protein of unknown function Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C C HDA; IDA; HDA cell periphery; fungal-type cell wall; fungal-type vacuole GO_0071944; GO_0009277; GO_0000324 456 YBR162W-A YSY6 Protein of unknown function Protein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion C HDA endoplasmic reticulum GO_0005783 457 YBR163W EXO5 Mitochondrial 5'-3' exonuclease and sliding exonuclease Mitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 C HDA mitochondrion GO_0005739 458 YBR164C ARL1 Soluble GTPase with a role in regulation of membrane traffic Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; G protein of the Ras superfamily, similar to ADP-ribosylation factor C IDA; IDA; IDA trans-Golgi network; Golgi apparatus; cytosol GO_0005802; GO_0005794; GO_0005829 459 YBR165W UBS1 Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity C IDA nucleus GO_0005634 460 YBR166C TYR1 Prephenate dehydrogenase involved in tyrosine biosynthesis Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels C HDA cytoplasm GO_0005737 461 YBR167C POP7 Subunit of RNase MRP, nuclear RNase P and telomerase Subunit of both RNase MRP and nuclear RNase P; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs C IPI; IDA; IDA ribonuclease MRP complex; telomerase holoenzyme complex; nucleolar ribonuclease P complex GO_0000172; GO_0005697; GO_0005655 462 YBR168W PEX32 Peroxisomal integral membrane protein Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p C IDA integral component of peroxisomal membrane GO_0005779 463 YBR169C SSE2 Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 464 YBR170C NPL4 Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) C HDA; HDA; IDA; IDA; IDA; IDA; IDA; IDA nucleus; cytoplasm; Doa10p ubiquitin ligase complex; RQC complex; Hrd1p ubiquitin ligase ERAD-L complex; nuclear outer membrane-endoplasmic reticulum membrane network; Cdc48p-Npl4p-Vms1p AAA ATPase complex; VCP-NPL4-UFD1 AAA ATPase complex GO_0005634; GO_0005737; GO_0000837; GO_1990112; GO_0000839; GO_0042175; GO_0036266; GO_0034098 465 YBR171W SEC66 Non-essential subunit of Sec63 complex Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec72p C HDA; IDA; IPI; HDA endoplasmic reticulum; endoplasmic reticulum membrane; Sec62/Sec63 complex; cell periphery GO_0005783; GO_0005789; GO_0031207; GO_0071944 466 YBR172C SMY2 GYF domain protein GYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; HDA P-body; endoplasmic reticulum membrane; cytosol; extrinsic component of membrane; cytoplasm GO_0000932; GO_0005789; GO_0005829; GO_0019898; GO_0005737 467 YBR173C UMP1 Chaperone required for correct maturation of the 20S proteasome Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 468 YBR174C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective 469 YBR175W SWD3 Essential subunit of the COMPASS (Set1C) complex Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 C IPI Set1C/COMPASS complex GO_0048188 470 YBR176W ECM31 Ketopantoate hydroxymethyltransferase Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate C HDA mitochondrion GO_0005739 471 YBR177C EHT1 Acyl-coenzymeA:ethanol O-acyltransferase Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication C HDA; IDA; HDA mitochondrion; lipid particle; mitochondrial outer membrane GO_0005739; GO_0005811; GO_0005741 472 YBR178W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C 473 YBR179C FZO1 Mitofusin Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system C HDA; IDA; HDA; IDA mitochondrion; integral component of mitochondrial outer membrane; mitochondrial outer membrane; mitochondrial inner membrane GO_0005739; GO_0031307; GO_0005741; GO_0005743 474 YBR180W DTR1 Putative dityrosine transporter of the major facilitator superfamily Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation C IDA; HDA prospore membrane; cell periphery GO_0005628; GO_0071944 475 YBR181C RPS6B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication C HDA; IDA; IDA cytoplasm; small-subunit processome; cytosolic small ribosomal subunit GO_0005737; GO_0032040; GO_0022627 476 YBR182C SMP1 MADS-box transcription factor involved in osmotic stress response MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 477 YBR182C-A YBR182C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 478 YBR183W YPC1 Alkaline ceramidase Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; cortical endoplasmic reticulum GO_0005783; GO_0032541 479 YBR184W YBR184W Putative protein of unknown function Putative protein of unknown function; YBR184W is not an essential gene 480 YBR185C MBA1 Membrane-associated mitochondrial ribosome receptor Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 481 YBR186W PCH2 Hexameric ring ATPase that remodels chromosome axis protein Hop1p Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation C IDA nucleolus GO_0005730 482 YBR187W GDT1 Calcium and manganese transporter with higher affinity for Ca2+ Protein of unknown function involved in calcium homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion protein localizes to the vacuole; TMEM165, a human gene which causes Congenital Disorders of Glycosylation is orthologous and functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression reduced in a gcr1 null mutant C HDA; IDA; IDA; IDA fungal-type vacuole membrane; Golgi medial cisterna; fungal-type vacuole; cis-Golgi network GO_0000329; GO_0005797; GO_0000324; GO_0005801 483 YBR188C NTC20 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs C IDA; IDA U2-type catalytic step 1 spliceosome; Prp19 complex GO_0071006; GO_0000974 484 YBR189W RPS9B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication C HDA; IDA; IDA 90S preribosome; cytosolic small ribosomal subunit; small-subunit processome GO_0030686; GO_0022627; GO_0032040 485 YBR190W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W 486 YBR191W RPL21A Ribosomal 60S subunit protein L21A Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 487 YBR191W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 488 YBR192W RIM2 Mitochondrial pyrimidine nucleotide transporter Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family C HDA mitochondrion GO_0005739 489 YBR193C MED8 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C IDA core mediator complex GO_0070847 490 YBR194W AIM4 Protein proposed to be associated with the nuclear pore complex Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress C IDA cytoplasm GO_0005737 491 YBR195C MSI1 Subunit of chromatin assembly factor I (CAF-1) Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase C IDA; IDA; IDA cytoplasm; nucleus; CAF-1 complex GO_0005737; GO_0005634; GO_0033186 492 YBR196C PGI1 Glycolytic enzyme phosphoglucose isomerase Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation C HDA; HDA; HDA plasma membrane; mitochondrion; cytoplasm GO_0005886; GO_0005739; GO_0005737 493 YBR196C-A YBR196C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 494 YBR196C-B YBR196C-B Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 495 YBR197C YBR197C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 496 YBR198C TAF5 Subunit (90 kDa) of TFIID and SAGA complexes Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification C IDA; IDA; IDA SLIK (SAGA-like) complex; transcription factor TFIID complex; SAGA complex GO_0046695; GO_0005669; GO_0000124 497 YBR199W KTR4 Glycosyltransferase involved in protein glycosylation Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain C HDA fungal-type vacuole GO_0000324 498 YBR200W BEM1 Protein containing SH3-domains Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p C HDA; IDA; IDA; HDA; IDA; IDA mating projection tip; incipient cellular bud site; cellular bud tip; mitochondrion; site of polarized growth; cellular bud neck GO_0043332; GO_0000131; GO_0005934; GO_0005739; GO_0030427; GO_0005935 499 YBR200W-A YBR200W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 500 YBR201C-A MIN7 Putative protein of unknown function Putative protein of unknown function 501 YBR201W DER1 ER membrane protein that promotes export of misfolded polypeptides ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into the ER membrane and routing them to Hrd1p for ubiquitylation; N- and C- termini protrude into the cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1 C IDA; IDA Hrd1p ubiquitin ligase ERAD-L complex; endoplasmic reticulum membrane GO_0000839; GO_0005789 502 YBR202W MCM7 Component of the Mcm2-7 hexameric helicase complex Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA CMG complex; MCM core complex; nucleus; nuclear pre-replicative complex; replication fork protection complex; cytoplasm; MCM complex; DNA replication preinitiation complex GO_0071162; GO_0097373; GO_0005634; GO_0005656; GO_0031298; GO_0005737; GO_0042555; GO_0031261 503 YBR203W COS111 Protein required for antifungal drug ciclopirox olamine resistance Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 504 YBR204C LDH1 Serine hydrolase Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) C HDA; IDA endoplasmic reticulum; lipid particle GO_0005783; GO_0005811 505 YBR205W KTR3 Putative alpha-1,2-mannosyltransferase Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress C HDA; HDA; HDA; HDA fungal-type vacuole membrane; cytoplasm; fungal-type vacuole; nucleus GO_0000329; GO_0005737; GO_0000324; GO_0005634 506 YBR206W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 507 YBR207W FTH1 Putative high affinity iron transporter Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 508 YBR208C DUR1,2 Urea amidolyase Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 509 YBR209W YBR209W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene 510 YBR210W ERV15 Protein involved in export of proteins from the endoplasmic reticulum Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication C IGI; HDA ER to Golgi transport vesicle; endoplasmic reticulum GO_0030134; GO_0005783 511 YBR211C AME1 Essential kinetochore protein associated with microtubules and SPBs Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress C IDA; HDA; HDA; HDA; IDA kinetochore; spindle pole body; nucleus; cytoplasm; COMA complex GO_0000776; GO_0005816; GO_0005634; GO_0005737; GO_0000817 512 YBR212W NGR1 RNA binding protein that negatively regulates growth rate RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase C IDA; IDA; IDA; HDA perinuclear region of cytoplasm; P-body; cytoplasmic stress granule; cytoplasm GO_0048471; GO_0000932; GO_0010494; GO_0005737 513 YBR213W MET8 Bifunctional dehydrogenase and ferrochelatase Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis 514 YBR214W SDS24 Protein involved in cell separation during budding Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 515 YBR215W HPC2 Subunit of the HIR complex Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes C HDA; IDA nucleus; HIR complex GO_0005634; GO_0000417 516 YBR216C YBP1 Protein involved in cellular response to oxidative stress Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 517 YBR217W ATG12 Ubiquitin-like modifier involved in autophagy and the Cvt pathway Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p cojugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation C HDA; IDA cytosol; Atg12-Atg5-Atg16 complex GO_0005829; GO_0034274 518 YBR218C PYC2 Pyruvate carboxylase isoform Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication C IDA cytosol GO_0005829 519 YBR219C YBR219C Putative protein of unknown function Putative protein of unknown function; YBR219C is not an essential gene 520 YBR220C YBR220C Putative protein of unknown function Putative protein of unknown function; YBR220C is not an essential gene 521 YBR221C PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria C HDA; IDA; IDA mitochondrion; mitochondrial nucleoid; mitochondrial pyruvate dehydrogenase complex GO_0005739; GO_0042645; GO_0005967 522 YBR221W-A YBR221W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 523 YBR222C PCS60 Oxalyl-CoA synthetase Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase C HDA; IDA; IDA cytoplasm; peroxisomal membrane; peroxisomal matrix GO_0005737; GO_0005778; GO_0005782 524 YBR223C TDP1 Tyrosyl-DNA phosphodiesterase I Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI C IDA nucleus GO_0005634 525 YBR223W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps YBR223C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 526 YBR224W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 527 YBR225W YBR225W Putative protein of unknown function Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components 528 YBR226C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W 529 YBR227C MCX1 Non-proteolytic ATPase of the AAA family Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 530 YBR228W SLX1 Endonuclease involved in DNA recombination and repair Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p C IPI Slx1-Slx4 complex GO_0033557 531 YBR229C ROT2 Glucosidase II catalytic subunit Glucosidase II catalytic subunit; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations C IPI; HDA; HDA; IDA glucosidase II complex; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen GO_0017177; GO_0005739; GO_0005783; GO_0005788 532 YBR230C OM14 Mitochondrial outer membrane receptor for cytosolic ribosomes Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron C HDA; IDA mitochondrion; integral component of mitochondrial outer membrane GO_0005739; GO_0031307 533 YBR230W-A COQ21 Putative protein of unknown function Putative protein of unknown function; YBR230W-A has a paralog, COQ8, that arose from the whole genome duplication 534 YBR231C SWC5 Component of the SWR1 complex Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA nucleus; cytosol; Swr1 complex GO_0005634; GO_0005829; GO_0000812 535 YBR232C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 536 YBR233W PBP2 RNA binding protein RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 537 YBR233W-A DAD3 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 538 YBR234C ARC40 Subunit of the ARP2/3 complex Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches C IDA Arp2/3 protein complex GO_0005885 539 YBR235W VHC1 Vacuolar membrane cation-chloride cotransporter (CCC) Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family C HDA; IDA; IDA fungal-type vacuole membrane; integral component of vacuolar membrane; vacuole-mitochondrion membrane contact site GO_0000329; GO_0031166; GO_1990816 540 YBR236C ABD1 Methyltransferase Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; nucleus; DNA-directed RNA polymerase II, holoenzyme GO_0005829; GO_0005634; GO_0016591 541 YBR237W PRP5 RNA helicase in the DEAD-box family RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA C HDA nucleus GO_0005634 542 YBR238C YBR238C Mitochondrial membrane protein Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication C HDA; IDA; IDA; HDA mitochondrion; integral component of mitochondrial membrane; P-body; cytoplasm GO_0005739; GO_0032592; GO_0000932; GO_0005737 543 YBR239C ERT1 Transcriptional regulator Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 544 YBR240C THI2 Transcriptional activator of thiamine biosynthetic genes Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type C IDA nucleus GO_0005634 545 YBR241C YBR241C Putative transporter, member of the sugar porter family Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication C IDA fungal-type vacuole membrane GO_0000329 546 YBR242W YBR242W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 547 YBR243C ALG7 UDP-N-acetyl-glucosamine-1-P transferase UDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin C HDA; IDA endoplasmic reticulum; UDP-N-acetylglucosamine transferase complex GO_0005783; GO_0043541 548 YBR244W GPX2 Phospholipid hydroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; HDA cytoplasm; extrinsic component of mitochondrial inner membrane; extrinsic component of mitochondrial outer membrane; nucleus GO_0005737; GO_0031314; GO_0031315; GO_0005634 549 YBR245C ISW1 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK C IDA; IDA; HDA; IDA nucleolar chromatin; Isw1b complex; nucleus; Isw1a complex GO_0030874; GO_0036437; GO_0005634; GO_0036436 550 YBR246W RRT2 Methylesterase performing penultimate step of diphthamide biosynthesis Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins C IDA; IDA cytoplasm; endosome GO_0005737; GO_0005768 551 YBR247C ENP1 Protein associated with U3 and U14 snoRNAs Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus C IDA; IDA; HDA; IDA; IDA preribosome, small subunit precursor; cytoplasm; 90S preribosome; nucleus; nucleolus GO_0030688; GO_0005737; GO_0030686; GO_0005634; GO_0005730 552 YBR248C HIS7 Imidazole glycerol phosphate synthase Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor 553 YBR249C ARO4 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 554 YBR250W SPO23 Protein of unknown function Protein of unknown function; associates with meiosis-specific protein Spo1p 555 YBR251W MRPS5 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 556 YBR252W DUT1 Deoxyuridine triphosphate diphosphatase (dUTPase) deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 557 YBR253W SRB6 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C IDA core mediator complex GO_0070847 558 YBR254C TRS20 Core component of transport protein particle (TRAPP) complexes I-III Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation of conserved residues leads to defects in endocytic recycling and a block in sporulation/meiosis; mutations in the human homolog cause the spondyloepiphyseal dysplasia C IDA; IDA; IDA TRAPPIII protein complex; TRAPPII protein complex; TRAPPI protein complex GO_1990072; GO_1990071; GO_1990070 559 YBR255C-A RCF3 Putative protein of unknown function Putative protein of unknown function; may interact with respiratory chain complexes III (ubiquinol-cytochrome c reductase) or IV (cytochrome c oxidase); identified by sequence comparison with hemiascomycetous yeast species 560 YBR255W MTC4 Protein of unknown function Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 C IDA; HDA; IDA lipid particle; cytoplasm; peroxisome GO_0005811; GO_0005737; GO_0005777 561 YBR256C RIB5 Riboflavin synthase Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway 562 YBR257W POP4 Subunit of both RNase MRP and nuclear RNase P Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P C IDA; IDA; IDA ribonuclease MRP complex; ribonuclease P complex; nucleolar ribonuclease P complex GO_0000172; GO_0030677; GO_0005655 563 YBR258C SHG1 Subunit of the COMPASS (Set1C) complex Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres C IPI; HDA Set1C/COMPASS complex; nucleus GO_0048188; GO_0005634 564 YBR259W YBR259W Protein of unknown function Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress 565 YBR260C RGD1 GTPase-activating protein (RhoGAP) for Rho3p and Rho4p GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization C HDA; IDA; HDA; IDA mating projection tip; actin cortical patch; prospore membrane; cellular bud GO_0043332; GO_0030479; GO_0005628; GO_0005933 566 YBR261C TAE1 AdoMet-dependent proline methyltransferase AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm C IDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 567 YBR262C MIC12 Component of the MICOS complex Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane C HDA; IDA; IDA mitochondrion; MICOS complex; mitochondrial crista junction GO_0005739; GO_0061617; GO_0044284 568 YBR263W SHM1 Mitochondrial serine hydroxymethyltransferase Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine C IMP mitochondrion GO_0005739 569 YBR264C YPT10 Rab family GTP-binding protein Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles C HDA; HDA cytoplasm; fungal-type vacuole membrane GO_0005737; GO_0000329 570 YBR265W TSC10 3-ketosphinganine reductase 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family C IDA; HDA; HDA; HDA lipid particle; cytoplasm; endoplasmic reticulum; mitochondrial outer membrane GO_0005811; GO_0005737; GO_0005783; GO_0005741 571 YBR266C Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase 572 YBR267W REI1 Cytoplasmic pre-60S factor Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network C IDA; IDA; IDA preribosome, large subunit precursor; cytoplasm; cytosolic large ribosomal subunit GO_0030687; GO_0005737; GO_0022625 573 YBR268W MRPL37 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 574 YBR269C SDH8 Protein required for assembly of succinate dehydrogenase Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 575 YBR270C BIT2 Subunit of TORC2 membrane-associated complex Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication C IDA TORC2 complex GO_0031932 576 YBR271W EFM2 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis C HDA cytoplasm GO_0005737 577 YBR272C HSM3 Evolutionarily conserved 19S regulatory particle assembly-chaperone Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation C IDA; IDA; IDA; HDA cytosol; nucleus; proteasome regulatory particle, base subcomplex; cytoplasm GO_0005829; GO_0005634; GO_0008540; GO_0005737 578 YBR273C UBX7 UBX (ubiquitin regulatory X) domain-containing protein UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; nuclear envelope GO_0005783; GO_0005635 579 YBR274W CHK1 Serine/threonine kinase and DNA damage checkpoint effector Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase C IPI nucleus GO_0005634 580 YBR275C RIF1 Protein that binds to the Rap1p C-terminus Protein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation C IPI; IDA shelterin complex; nuclear chromosome, telomeric region GO_0070187; GO_0000784 581 YBR276C PPS1 Protein phosphatase Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle 582 YBR277C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W 583 YBR278W DPB3 Third-largest subunit of DNA polymerase II (DNA polymerase epsilon) Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication C IDA epsilon DNA polymerase complex GO_0008622 584 YBR279W PAF1 Component of the Paf1p complex involved in transcription elongation Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 C IPI; IDA; IDA Cdc73/Paf1 complex; nucleus; transcriptionally active chromatin GO_0016593; GO_0005634; GO_0035327 585 YBR280C SAF1 F-Box protein involved in proteasome-dependent degradation of Aah1p F-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 C IMP SCF ubiquitin ligase complex GO_0019005 586 YBR281C DUG2 Component of glutamine amidotransferase (GATase II) Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) C IDA; HDA; IDA; IDA cytoplasm; nucleus; nuclear periphery; glutathione hydrolase complex GO_0005737; GO_0005634; GO_0034399; GO_0061672 587 YBR282W MRPL27 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 588 YBR283C SSH1 Subunit of the Ssh1 translocon complex Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential C IDA; HDA Ssh1 translocon complex; endoplasmic reticulum GO_0071261; GO_0005783 589 YBR284W YBR284W Putative metallo-dependent hydrolase superfamily protein Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication 590 YBR285W YBR285W Putative protein of unknown function Putative protein of unknown function; YBR285W is not an essential gene 591 YBR286W APE3 Vacuolar aminopeptidase Y Vacuolar aminopeptidase Y; processed to mature form by Prb1p C IDA fungal-type vacuole GO_0000324 592 YBR287W YBR287W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene C HDA endoplasmic reticulum GO_0005783 593 YBR288C APM3 Mu3-like subunit of the clathrin associated protein complex (AP-3) Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway C IDA AP-3 adaptor complex GO_0030123 594 YBR289W SNF5 Subunit of the SWI/SNF chromatin remodeling complex Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions C IDA; IMP; IDA nucleus; SWI/SNF complex; cytosol GO_0005634; GO_0016514; GO_0005829 595 YBR290W BSD2 Heavy metal ion homeostasis protein Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification C IDA; IDA; IDA fungal-type vacuole; endoplasmic reticulum; fungal-type vacuole membrane GO_0000324; GO_0005783; GO_0000329 596 YBR291C CTP1 Mitochondrial inner membrane citrate transporter Mitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family C HDA mitochondrion GO_0005739 597 YBR292C YBR292C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene 598 YBR293W VBA2 Permease of basic amino acids in the vacuolar membrane Permease of basic amino acids in the vacuolar membrane C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 599 YBR294W SUL1 High affinity sulfate permease of the SulP anion transporter family High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates C IMP plasma membrane GO_0005886 600 YBR295W PCA1 Cadmium transporting P-type ATPase Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function C IDA; IDA; IDA proteasome core complex; integral component of plasma membrane; plasma membrane GO_0005839; GO_0005887; GO_0005886 601 YBR296C PHO89 Plasma membrane Na+/Pi cotransporter Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification C HDA; IMP endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 602 YBR296C-A YBR296C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 603 YBR297W MAL33 MAL-activator protein MAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C 604 YBR298C MAL31 Maltose permease Maltose permease; high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C C HDA cell periphery GO_0071944 605 YBR298C-A YBR298C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 606 YBR299W MAL32 Maltase (alpha-D-glucosidase) Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose C HDA plasma membrane GO_0005886 607 YBR300C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene 608 YBR301W PAU24 Cell wall mannoprotein Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth 609 YBR302C COS2 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA; IDA fungal-type vacuole; endosome GO_0000324; GO_0005768 610 YCL001W RER1 Protein involved in retention of membrane proteins Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER C IDA; IDA; IDA ER to Golgi transport vesicle; COPI-coated vesicle; fungal-type vacuole GO_0030134; GO_0030137; GO_0000324 611 YCL001W-A YCL001W-A Putative protein of unknown function Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III 612 YCL001W-B YCL001W-B Putative protein of unknown function Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication 613 YCL002C YCL002C Putative protein of unknown function Putative protein of unknown function; YCL002C is not an essential gene C HDA; HDA nucleus; cytosol GO_0005634; GO_0005829 614 YCL004W PGS1 Phosphatidylglycerolphosphate synthase Phosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis C IDA mitochondrion GO_0005739 615 YCL005W LDB16 Protein involved in lipid droplet (LD) assembly Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria C HDA; IDA; HDA mitochondrion; integral component of endoplasmic reticulum membrane; lipid particle GO_0005739; GO_0030176; GO_0005811 616 YCL005W-A VMA9 Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis C IMP; IMP vacuolar proton-transporting V-type ATPase, V0 domain; fungal-type vacuole membrane GO_0000220; GO_0000329 617 YCL007C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A 618 YCL008C STP22 Component of the ESCRT-I complex Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype; C IPI; IDA; IDA; HDA ESCRT I complex; endosome; cytoplasmic side of plasma membrane; cytosol GO_0000813; GO_0005768; GO_0009898; GO_0005829 619 YCL009C ILV6 Regulatory subunit of acetolactate synthase Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria C HDA; IDA; IDA mitochondrion; mitochondrial nucleoid; acetolactate synthase complex GO_0005739; GO_0042645; GO_0005948 620 YCL010C SGF29 Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation C IDA; IPI SAGA complex; Ada2/Gcn5/Ada3 transcription activator complex GO_0000124; GO_0005671 621 YCL011C GBP2 Poly(A+) RNA-binding protein Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; cytoplasmic stress granule; cytosol GO_0005634; GO_0010494; GO_0005829 622 YCL012C YCL012C Protein of unknown function Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene 623 YCL014W BUD3 Guanine nucleotide exchange factor (GEF) for Cdc42p Protein involved in bud-site selection; required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding C IDA; IDA cellular bud neck contractile ring; cellular bud neck GO_0000142; GO_0005935 624 YCL016C DCC1 Subunit of a complex with Ctf8p and Ctf18p Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance C IPI Ctf18 RFC-like complex GO_0031390 625 YCL017C NFS1 Cysteine desulfurase Cysteine desulfurase; involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria C IMP; IPI; HDA nucleus; L-cysteine desulfurase complex; mitochondrion GO_0005634; GO_1990221; GO_0005739 626 YCL018W LEU2 Beta-isopropylmalate dehydrogenase (IMDH) Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of β-ethylmalate into α-ketovalerate C IDA cytosol GO_0005829 627 YCL019W YCL019W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 628 YCL020W YCL020W Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 629 YCL021W-A YCL021W-A Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole GO_0000324 630 YCL022C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W 631 YCL023C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 632 YCL024W KCC4 Protein kinase of the bud neck involved in the septin checkpoint Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA cellular bud neck septin collar; cellular bud neck; incipient cellular bud site; septin ring; plasma membrane GO_0032174; GO_0005935; GO_0000131; GO_0005940; GO_0005886 633 YCL025C AGP1 Low-affinity amino acid permease with broad substrate range Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 634 YCL026C-A FRM2 Type II nitroreductase, using NADH as reductant Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 635 YCL026C-B HBN1 Protein of unknown function Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 636 YCL027W FUS1 Membrane protein localized to the shmoo tip Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate C IDA; IDA; HDA mating projection tip; integral component of plasma membrane; endoplasmic reticulum GO_0043332; GO_0005887; GO_0005783 637 YCL028W RNQ1 [PIN(+)] prion [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate C IDA cytosol GO_0005829 638 YCL029C BIK1 Microtubule-associated protein Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA condensed nuclear chromosome kinetochore; spindle pole body; microtubule plus-end; kinetochore; spindle; cell cortex; mating projection tip; spindle microtubule GO_0000778; GO_0005816; GO_0035371; GO_0000776; GO_0005819; GO_0005938; GO_0043332; GO_0005876 639 YCL030C HIS4 Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis C HDA cytoplasm GO_0005737 640 YCL031C RRP7 Essential protein involved in rRNA processing and ribosome biogenesis Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA; IDA nucleus; UTP-C complex; nucleolus; CURI complex GO_0005634; GO_0034456; GO_0005730; GO_0032545 641 YCL032W STE50 Adaptor protein for various signaling pathways Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction C IDA cytoplasm GO_0005737 642 YCL033C MXR2 Methionine-R-sulfoxide reductase Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan C IDA mitochondrion GO_0005739 643 YCL034W LSB5 Protein involved in membrane-trafficking events at plasma membrane Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat C IDA; IDA; HDA; IDA actin cortical patch; cell cortex; cytosol; cytoplasm GO_0030479; GO_0005938; GO_0005829; GO_0005737 644 YCL035C GRX1 Glutathione-dependent disulfide oxidoreductase Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 645 YCL036W GFD2 Protein of unknown function Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication 646 YCL037C SRO9 Cytoplasmic RNA-binding protein Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication C IDA; IDA polysome; cytoplasmic stress granule GO_0005844; GO_0010494 647 YCL038C ATG22 Vacuolar integral membrane protein required for efflux of amino acids Vacuolar integral membrane protein required for efflux of amino acids; required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation C IDA integral component of fungal-type vacuolar membrane GO_0071627 648 YCL039W GID7 Subunit of GID Complex that binds directly to central component Vid30p Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions C HDA; IDA; HDA nucleus; GID complex; cytoplasm GO_0005634; GO_0034657; GO_0005737 649 YCL040W GLK1 Glucokinase Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication C HDA; HDA plasma membrane; cytosol GO_0005886; GO_0005829 650 YCL041C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W 651 YCL042W YCL042W Putative protein of unknown function Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 652 YCL043C PDI1 Protein disulfide isomerase Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication C IDA; HDA endoplasmic reticulum lumen; endoplasmic reticulum GO_0005788; GO_0005783 653 YCL044C MGR1 Subunit of the mitochondrial (mt) i-AAA protease supercomplex Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA C HDA; IDA mitochondrion; i-AAA complex GO_0005739; GO_0031942 654 YCL045C EMC1 Member of conserved endoplasmic reticulum membrane complex Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 C IDA; HDA ER membrane protein complex; endoplasmic reticulum GO_0072546; GO_0005783 655 YCL046W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C 656 YCL047C POF1 Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT) Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 657 YCL048W SPS22 Protein of unknown function Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall C HDA cytosol GO_0005829 658 YCL048W-A YCL048W-A Putative protein of unknown function Putative protein of unknown function; YCL048W-A has a paralog, YDR524C-B, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 659 YCL049C YCL049C Protein of unknown function Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene C IDA membrane GO_0016020 660 YCL050C APA1 AP4A phosphorylase AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 661 YCL051W LRE1 Protein involved in control of cell wall structure and stress response Protein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication C IDA cellular bud neck GO_0005935 662 YCL052C PBN1 Component of glycosylphosphatidylinositol-mannosyltransferase I Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X C IDA; IPI endoplasmic reticulum; glycosylphosphatidylinositol-mannosyltransferase I complex GO_0005783; GO_1990529 663 YCL054W SPB1 AdoMet-dependent methyltransferase AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants C IDA; IDA; IDA preribosome, large subunit precursor; nucleus; nucleolus GO_0030687; GO_0005634; GO_0005730 664 YCL055W KAR4 Transcription factor required for response to pheromones Transcription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone C HDA; HDA; HDA cytosol; cytoplasm; nucleus GO_0005829; GO_0005737; GO_0005634 665 YCL056C PEX34 Protein that regulates peroxisome populations Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation C HDA; IDA cytoplasm; integral component of peroxisomal membrane GO_0005737; GO_0005779 666 YCL057C-A MIC10 Conserved component of the MICOS complex Conserved component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; ortholog of human MINOS1 C HDA; IDA; IDA mitochondrion; MICOS complex; mitochondrial crista junction GO_0005739; GO_0061617; GO_0044284 667 YCL057W PRD1 Zinc metalloendopeptidase Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA; IDA; IDA fungal-type vacuole; mitochondrion; cytoplasm; Golgi apparatus; mitochondrial intermembrane space GO_0000324; GO_0005739; GO_0005737; GO_0005794; GO_0005758 668 YCL058C FYV5 Protein involved in regulation of the mating pathway Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis C HDA cytosol GO_0005829 669 YCL058W-A ADF1 Transcriptional repressor encoded by the FYV5 antisense strand Transcriptional repressor encoded by the FYV5 antisense strand; negatively regulates transcription of FYV5 by binding to the promoter on the sense strand C IDA nucleus GO_0005634 670 YCL059C KRR1 Nucleolar protein required for rRNA synthesis and ribosomal assembly Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene C IDA; IDA; HDA nucleolus; small-subunit processome; 90S preribosome GO_0005730; GO_0032040; GO_0030686 671 YCL061C MRC1 S-phase checkpoint protein required for DNA replication S-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle C IDA; IDA; IPI; HDA; IDA nuclear replication fork; replication fork protection complex; nuclear chromosome; nucleus; nuclear periphery GO_0043596; GO_0031298; GO_0000228; GO_0005634; GO_0034399 672 YCL063W VAC17 Phosphoprotein involved in vacuole inheritance Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p C IPI; IDA Myo2p-Vac17p-Vac8p transport complex; fungal-type vacuole membrane GO_0071563; GO_0000329 673 YCL064C CHA1 Catabolic L-serine (L-threonine) deaminase Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 674 YCL065W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps HMLALPHA1 675 YCL066W HMLALPHA1 Silenced copy of ALPHA1 at HML Silenced copy of ALPHA1 at HML; ALPHA1 encodes a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression 676 YCL067C HMLALPHA2 Silenced copy of ALPHA2 at HML Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression 677 YCL068C YCL068C Putative protein of unknown function Putative protein of unknown function 678 YCL069W VBA3 Permease of basic amino acids in the vacuolar membrane Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 679 YCL073C GEX1 Proton:glutathione antiporter Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; imports glutathione from the vacuole and exports it through the plasma membrane; has a role in resistance to oxidative stress and modulation of the PKA pathway; GEX1 has a paralog, GEX2, that arose from a segmental duplication C IDA; IDA; HDA plasma membrane; vacuolar membrane; cell periphery GO_0005886; GO_0005774; GO_0071944 680 YCL076W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 681 YCR001W YCR001W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene 682 YCR002C CDC10 Component of the septin ring, required for cytokinesis Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress C HDA; IDA; IPI; IDA; HDA; IDA; IDA; HDA; IDA; IDA cellular bud neck; septin filament array; septin complex; ascospore-type prospore; prospore membrane; cellular bud neck septin ring; meiotic spindle; mating projection tip; mating projection base; spindle microtubule GO_0005935; GO_0032160; GO_0031105; GO_0042764; GO_0005628; GO_0000144; GO_0072687; GO_0043332; GO_0001400; GO_0005876 683 YCR003W MRPL32 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA mitochondrial inner membrane; mitochondrion; mitochondrial large ribosomal subunit GO_0005743; GO_0005739; GO_0005762 684 YCR004C YCP4 Protein of unknown function Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; IDA; HDA; HDA; IDA cell periphery; cytoplasm; plasma membrane; mitochondrion; membrane raft GO_0071944; GO_0005737; GO_0005886; GO_0005739; GO_0045121 685 YCR005C CIT2 Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication C IDA; HDA; IDA cytoplasm; mitochondrion; peroxisome GO_0005737; GO_0005739; GO_0005777 686 YCR006C YCR006C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 687 YCR007C YCR007C Putative integral membrane protein Putative integral membrane protein; member of DUP240 gene family; YCR007C is not an essential gene C HDA; HDA cell periphery; fungal-type vacuole GO_0071944; GO_0000324 688 YCR008W SAT4 Ser/Thr protein kinase involved in salt tolerance Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion C IDA; IDA mitochondrion; cytoplasm GO_0005739; GO_0005737 689 YCR009C RVS161 Amphiphysin-like lipid raft protein Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress C IDA; HDA; IDA; IDA; IPI; HDA mating projection; mating projection tip; membrane raft; actin cortical patch; Rvs161p-Rvs167p complex; cytosol GO_0005937; GO_0043332; GO_0045121; GO_0030479; GO_1990528; GO_0005829 690 YCR010C ADY2 Acetate transporter required for normal sporulation Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication C HDA; IDA mitochondrion; plasma membrane GO_0005739; GO_0005886 691 YCR011C ADP1 Putative ATP-dependent permease of the ABC transporter family Putative ATP-dependent permease of the ABC transporter family C HDA; HDA; HDA; HDA fungal-type vacuole; fungal-type vacuole membrane; cytoplasm; endoplasmic reticulum GO_0000324; GO_0000329; GO_0005737; GO_0005783 692 YCR012W PGK1 3-phosphoglycerate kinase 3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis C HDA; IDA; IDA plasma membrane; cytoplasm; mitochondrion GO_0005886; GO_0005737; GO_0005739 693 YCR013C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant 694 YCR014C POL4 DNA polymerase IV DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta 695 YCR015C CTO1 Protein required for cold tolerance Putative protein of unknown function; YCR015C is not an essential gene 696 YCR016W YCR016W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis C HDA; HDA nucleus; nucleolus GO_0005634; GO_0005730 697 YCR017C CWH43 GPI lipid remodelase Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion C IDA; HDA; IDA; IDA cellular bud tip; endoplasmic reticulum; cellular bud neck; integral component of plasma membrane GO_0005934; GO_0005783; GO_0005935; GO_0005887 698 YCR018C SRD1 Protein involved in the processing of pre-rRNA to mature rRNA Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 699 YCR018C-A Putative protein of unknown function; encoded opposite a Ty1 LTR 700 YCR019W MAK32 Protein necessary for stability of L-A dsRNA-containing particles Protein necessary for stability of L-A dsRNA-containing particles 701 YCR020C PET18 Protein of unknown function Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin C IDA cytosol GO_0005829 702 YCR020C-A MAK31 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family C IDA NatC complex GO_0031417 703 YCR020W-B HTL1 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance C IDA RSC complex GO_0016586 704 YCR021C HSP30 Negative regulator of the H(+)-ATPase Pma1p Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase C IDA; HDA plasma membrane; endoplasmic reticulum GO_0005886; GO_0005783 705 YCR022C YCR022C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene 706 YCR023C YCR023C Vacuolar membrane protein of unknown function Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene C IDA fungal-type vacuole membrane GO_0000329 707 YCR024C SLM5 Mitochondrial asparaginyl-tRNA synthetase Mitochondrial asparaginyl-tRNA synthetase C HDA mitochondrion GO_0005739 708 YCR024C-A PMP1 Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication C IDA; IDA membrane; plasma membrane GO_0016020; GO_0005886 709 YCR024C-B YCR024C-B Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 710 YCR025C YCR025C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene 711 YCR026C NPP1 Nucleotide pyrophosphatase/phosphodiesterase Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants 712 YCR027C RHB1 Putative Rheb-related GTPase Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins C IGI; HDA extrinsic component of plasma membrane; cytosol GO_0019897; GO_0005829 713 YCR028C FEN2 Plasma membrane H+-pantothenate symporter Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress C HDA; IGI; HDA; HDA cell periphery; plasma membrane; fungal-type vacuole; fungal-type vacuole membrane GO_0071944; GO_0005886; GO_0000324; GO_0000329 714 YCR028C-A RIM1 ssDNA-binding protein essential for mitochondrial genome maintenance ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 715 YCR030C SYP1 Negative regulator of WASP-Arp23 complex Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization C IDA; HDA; HDA; IDA; IDA; IDA; IDA cellular bud neck; mating projection tip; prospore membrane; mating projection base; endocytic patch; cellular bud tip; cellular bud neck septin ring GO_0005935; GO_0043332; GO_0005628; GO_0001400; GO_0061645; GO_0005934; GO_0000144 716 YCR031C RPS14A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication C HDA; IDA 90S preribosome; small-subunit processome GO_0030686; GO_0032040 717 YCR032W BPH1 Protein homologous to Chediak-Higashi syndrome and Beige proteins Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting C IDA; HDA; IDA; HDA extrinsic component of membrane; mitochondrion; cytosol; fungal-type vacuole membrane GO_0019898; GO_0005739; GO_0005829; GO_0000329 718 YCR033W SNT1 Subunit of the Set3C deacetylase complex Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia C IDA; HDA; IDA; IDA cytosol; Rpd3L-Expanded complex; Set3 complex; nucleus GO_0005829; GO_0070210; GO_0034967; GO_0005634 719 YCR034W ELO2 Fatty acid elongase, involved in sphingolipid biosynthesis Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 720 YCR035C RRP43 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA; IDA nuclear exosome (RNase complex); nucleoplasm; cytoplasm; cytoplasmic exosome (RNase complex); nucleolus GO_0000176; GO_0005654; GO_0005737; GO_0000177; GO_0005730 721 YCR036W RBK1 Putative ribokinase Putative ribokinase C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 722 YCR037C PHO87 Low-affinity inorganic phosphate (Pi) transporter Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 723 YCR038C BUD5 GTP/GDP exchange factor for Rsr1p (Bud1p) GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types C IDA; IDA incipient cellular bud site; cellular bud neck GO_0000131; GO_0005935 724 YCR038W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene BUD5/YCR038C; identified by homology with hemiascomycetous yeast species 725 YCR039C MATALPHA2 Homeobox-domain protein Homeobox-domain protein; with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette C IDA nucleus GO_0005634 726 YCR040W MATALPHA1 Transcriptional co-activator that regulates mating-type-specific genes Transcriptional co-activator that regulates mating-type-specific genes; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette 727 YCR041W YCR041W Protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 728 YCR042C TAF2 TFIID subunit (150 kDa) TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation C IDA transcription factor TFIID complex GO_0005669 729 YCR043C YCR043C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene C HDA; HDA; HDA mating projection tip; Golgi apparatus; cytosol GO_0043332; GO_0005794; GO_0005829 730 YCR044C PER1 Protein of the endoplasmic reticulum Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 C IDA; IDA fungal-type vacuole membrane; endoplasmic reticulum GO_0000329; GO_0005783 731 YCR045C RRT12 Probable subtilisin-family protease Probable subtilisin-family protease; role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores C IDA; IDA ascospore wall; nuclear envelope GO_0005619; GO_0005635 732 YCR045W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with YCR045C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 733 YCR046C IMG1 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 734 YCR047C BUD23 Methyltransferase that methylates residue G1575 of 18S rRNA Methyltransferase; methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern C HDA; IDA; HDA cytoplasm; nucleolus; nucleus GO_0005737; GO_0005730; GO_0005634 735 YCR047W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with YCR047C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 736 YCR048W ARE1 Acyl-CoA:sterol acyltransferase Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 737 YCR049C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 738 YCR050C YCR050C Non-essential protein of unknown function Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein 739 YCR051W YCR051W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 740 YCR052W RSC6 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication C IDA RSC complex GO_0016586 741 YCR053W THR4 Threonine synthase Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 742 YCR054C CTR86 Essential protein of unknown function Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 743 YCR057C PWP2 Conserved 90S pre-ribosomal component Conserved 90S pre-ribosomal component; essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis C HDA; IDA; IDA; IDA; IDA 90S preribosome; nucleolus; Pwp2p-containing subcomplex of 90S preribosome; cytoplasm; small-subunit processome GO_0030686; GO_0005730; GO_0034388; GO_0005737; GO_0032040 744 YCR059C YIH1 Negative regulator of eIF2 kinase Gcn2p Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT C IDA; IDA; HDA; HDA ribosome; polysome; nucleus; cytoplasm GO_0005840; GO_0005844; GO_0005634; GO_0005737 745 YCR060W TAH1 Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1) Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants C IPI; HDA; HDA R2TP complex; cytoplasm; nucleus GO_0097255; GO_0005737; GO_0005634 746 YCR061W YCR061W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation C HDA cytoplasm GO_0005737 747 YCR063W BUD31 Component of the SF3b subcomplex of the U2 snRNP Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis C HDA; IDA nucleus; U2 snRNP GO_0005634; GO_0005686 748 YCR064C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BUD31 749 YCR065W HCM1 Forkhead transcription factor Forkhead transcription factor; drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role; regulates replicative lifespan; ortholog of C. elegans lifespan regulator PHA-4 C IDA; IDA; IDA nuclear chromatin; cytoplasm; nucleus GO_0000790; GO_0005737; GO_0005634 750 YCR066W RAD18 E3 ubiquitin ligase E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA C IDA; IDA; IDA Rad6-Rad18 complex; nuclear chromatin; nucleus GO_0097505; GO_0000790; GO_0005634 751 YCR067C SED4 Integral ER membrane protein that stimulates Sar1p GTPase activity Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication C HDA; IDA cytoplasm; integral component of endoplasmic reticulum membrane GO_0005737; GO_0030176 752 YCR068W ATG15 Phospholipase Lipase required for intravacuolar lysis of autophagic and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway C HDA; IDA; IDA endoplasmic reticulum; vacuolar lumen; integral component of membrane GO_0005783; GO_0005775; GO_0016021 753 YCR069W CPR4 Peptidyl-prolyl cis-trans isomerase (cyclophilin) Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 754 YCR071C IMG2 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49 C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 755 YCR072C RSA4 WD-repeat protein involved in ribosome biogenesis WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus C HDA; IDA ribosome; nucleolus GO_0005840; GO_0005730 756 YCR073C SSK22 MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication 757 YCR073W-A SOL2 Protein with a possible role in tRNA export Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication C HDA; IDA cytoplasm; cytosol GO_0005737; GO_0005829 758 YCR075C ERS1 Protein involved in cystine transport Protein with similarity to human cystinosin; cystinosin is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains C IDA; IDA; IDA fungal-type vacuole; endosome; plasma membrane GO_0000324; GO_0005768; GO_0005886 759 YCR075W-A EGO2 Subunit of the EGO/GSE complex Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication C IPI; IDA EGO complex; fungal-type vacuole membrane GO_0034448; GO_0000329 760 YCR076C FUB1 Proteasome-binding protein Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene 761 YCR077C PAT1 Deadenylation-dependent mRNA-decapping factor Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation C IDA; IDA; IDA; IMP; HDA; IDA; IDA cytoplasmic stress granule; nucleus; kinetochore; P-body; cytoplasm; Lsm1-7-Pat1 complex; cytosolic small ribosomal subunit GO_0010494; GO_0005634; GO_0000776; GO_0000932; GO_0005737; GO_1990726; GO_0022627 762 YCR079W PTC6 Mitochondrial type 2C protein phosphatase (PP2C) Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase C HDA; IDA; IDA mitochondrion; mitochondrial intermembrane space; mitochondrial matrix GO_0005739; GO_0005758; GO_0005759 763 YCR081C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with SRB8/YCR081W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 764 YCR081W SRB8 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression C IDA mediator complex GO_0016592 765 YCR082W AHC2 Component of the ADA histone acetyltransferase complex Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex C IPI; HDA; HDA Ada2/Gcn5/Ada3 transcription activator complex; nucleus; cytoplasm GO_0005671; GO_0005634; GO_0005737 766 YCR083W TRX3 Mitochondrial thioredoxin Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p C IDA mitochondrion GO_0005739 767 YCR084C TUP1 General repressor of transcription General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes C IDA; IPI nucleus; transcriptional repressor complex GO_0005634; GO_0017053 768 YCR085W YCR085W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 769 YCR086W CSM1 Nucleolar protein that mediates homolog segregation during meiosis I Nucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation C IDA; IDA; IDA monopolin complex; nucleolus; nuclear envelope GO_0033551; GO_0005730; GO_0005635 770 YCR087C-A YCR087C-A Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene C HDA nucleolus GO_0005730 771 YCR087W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene 772 YCR088W ABP1 Actin-binding protein of the cortical actin cytoskeleton Actin-binding protein of the cortical actin cytoskeleton; important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; inhibits barbed-end actin filament elongation; phosphorylation within its Proline-Rich Regio, mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences; mammalian homologue of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa) C IDA; HDA; IDA; HDA actin cortical patch; mating projection tip; cell cortex; cytoplasm GO_0030479; GO_0043332; GO_0005938; GO_0005737 773 YCR089W FIG2 Cell wall adhesin, expressed specifically during mating Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication C IDA; IDA; HDA fungal-type cell wall; mating projection; cell periphery GO_0009277; GO_0005937; GO_0071944 774 YCR090C YCR090C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 775 YCR091W KIN82 Putative serine/threonine protein kinase Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; KIN82 has a paralog, FPK1, that arose from the whole genome duplication 776 YCR092C MSH3 Mismatch repair protein Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability C IPI; HDA; HDA MutSbeta complex; nucleus; cytoplasm GO_0032302; GO_0005634; GO_0005737 777 YCR093W CDC39 Subunit of the CCR4-NOT1 core complex Component of the CCR4-NOT1 core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor C IDA; IDA; IPI cytoplasm; nucleus; CCR4-NOT core complex GO_0005737; GO_0005634; GO_0030015 778 YCR094W CDC50 Endosomal protein that interacts with phospholipid flippase Drs2p Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication C HDA; IPI; IDA; IDA endoplasmic reticulum; Cdc50p-Drs2p complex; trans-Golgi network; late endosome membrane GO_0005783; GO_1990530; GO_0005802; GO_0031902 779 YCR095C OCA4 Cytoplasmic protein required for replication of Brome mosaic virus Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts C HDA cytoplasm GO_0005737 780 YCR095W-A YCR095W-A Putative protein of unknown function Putative protein of unknown function 781 YCR096C HMRA2 Silenced copy of a2 at HMR Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system 782 YCR097W HMRA1 Silenced copy of a1 at HMR Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells C IDA nucleus GO_0005634 783 YCR097W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by homology to a hemiascomycetous yeast protein 784 YCR098C GIT1 Plasma membrane permease Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability C IDA; HDA; HDA plasma membrane; fungal-type vacuole; cell periphery GO_0005886; GO_0000324; GO_0071944 785 YCR099C YCR099C Putative protein of unknown function Putative protein of unknown function 786 YCR100C YCR100C Putative protein of unknown function Putative protein of unknown function 787 YCR101C YCR101C Putative protein of unknown function Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene C IDA; HDA membrane; fungal-type vacuole GO_0016020; GO_0000324 788 YCR102C YCR102C Putative protein of unknown function Putative protein of unknown function; involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family 789 YCR102W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 790 YCR104W PAU3 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme 791 YCR105W ADH7 NADPH-dependent medium chain alcohol dehydrogenase NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance 792 YCR106W RDS1 Putative zinc cluster transcription factor Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide 793 YCR107W AAD3 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD3 has a paralog, AAD15, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 794 YCR108C YCR108C Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 795 YDL001W RMD1 Cytoplasmic protein required for sporulation Cytoplasmic protein required for sporulation C HDA; HDA cytoplasm; fungal-type vacuole membrane GO_0005737; GO_0000329 796 YDL002C NHP10 Non-essential INO80 chromatin remodeling complex subunit Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins C IDA; HDA Ino80 complex; nucleus GO_0031011; GO_0005634 797 YDL003W MCD1 Essential alpha-kleisin subunit of the cohesin complex Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase C IDA; IDA; IDA; IDA nuclear mitotic cohesin complex; condensed nuclear chromosome; mitochondrion; nucleus GO_0034990; GO_0000794; GO_0005739; GO_0005634 798 YDL004W ATP16 Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated C HDA; IDA mitochondrion; mitochondrial proton-transporting ATP synthase, central stalk GO_0005739; GO_0005756 799 YDL005C MED2 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA cytosol; nucleus; mediator complex; core mediator complex GO_0005829; GO_0005634; GO_0016592; GO_0070847 800 YDL006W PTC1 Type 2C protein phosphatase (PP2C) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 801 YDL007C-A YDL007C-A Putative protein of unknown function Putative protein of unknown function 802 YDL007W RPT2 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome C IDA; IDA proteasome regulatory particle, base subcomplex; nucleus GO_0008540; GO_0005634 803 YDL008W APC11 Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C) Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity C IDA anaphase-promoting complex GO_0005680 804 YDL009C YDL009C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene C HDA cytosol GO_0005829 805 YDL010W GRX6 Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA; IDA integral component of membrane; fungal-type vacuole; endoplasmic reticulum membrane; cis-Golgi network; Golgi apparatus; Golgi lumen GO_0016021; GO_0000324; GO_0005789; GO_0005801; GO_0005794; GO_0005796 806 YDL011C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W 807 YDL012C YDL012C Tail-anchored plasma membrane protein with a conserved CYSTM module Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication C HDA; HDA; IDA cellular bud; cell periphery; plasma membrane GO_0005933; GO_0071944; GO_0005886 808 YDL013W SLX5 Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase complex; stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers; the SUMO-targeted ubiquitin ligase complex is also known as the STUbL complex C IDA; IDA; IDA nucleus; SUMO-targeted ubiquitin ligase complex; chromosome, centromeric region GO_0005634; GO_0033768; GO_0000775 809 YDL014W NOP1 Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin C IDA; IDA; HDA; IDA; HDA nucleolus; box C/D snoRNP complex; ribosome; small-subunit processome; 90S preribosome GO_0005730; GO_0031428; GO_0005840; GO_0032040; GO_0030686 810 YDL015C TSC13 Enoyl reductase Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress C HDA; HDA; IDA endoplasmic reticulum; mitochondrion; endoplasmic reticulum membrane GO_0005783; GO_0005739; GO_0005789 811 YDL016C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication 812 YDL017W CDC7 Ser/Thr kinase and catalytic subunit of DDK (Dbf4-dependent kinase) DDK (Dbf4-dependent kinase) catalytic subunit; required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p C IDA; IDA; IDA; IDA Dbf4-dependent protein kinase complex; chromatin; nucleus; chromosome, centromeric region GO_0031431; GO_0000785; GO_0005634; GO_0000775 813 YDL018C ERP3 Protein with similarity to Emp24p and Erv25p Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport C HDA endoplasmic reticulum GO_0005783 814 YDL019C OSH2 Member of an oxysterol-binding protein family with seven members Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH2 has a paralog, SWH1, that arose from the whole genome duplication C HDA; IDA; IDA; IDA; IDA; IDA cell periphery; plasma membrane; nuclear envelope; cellular bud neck; cortical endoplasmic reticulum; endoplasmic reticulum GO_0071944; GO_0005886; GO_0005635; GO_0005935; GO_0032541; GO_0005783 815 YDL020C RPN4 Transcription factor that stimulates expression of proteasome genes Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 816 YDL021W GPM2 Nonfunctional homolog of Gpm1p phosphoglycerate mutase Homolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM2 has a paralog, GPM3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 817 YDL022C-A YDL022C-A Protein of unknown function Protein of unknown function; partially overlaps the verified gene DIA3; identified by fungal homology and RT-PCR; mRNA identified as translated by ribosome profiling data C HDA cytosol GO_0005829 818 YDL022W GPD1 NAD-dependent glycerol-3-phosphate dehydrogenase NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity; GPD1 has a paralog, GPD2, that arose from the whole genome duplication C HDA; IDA; IDA; IDA mitochondrion; peroxisome; nucleus; cytosol GO_0005739; GO_0005777; GO_0005634; GO_0005829 819 YDL023C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance 820 YDL024C DIA3 Protein of unknown function Protein of unknown function; involved in invasive and pseudohyphal growth C IDA; HDA fungal-type cell wall; cell periphery GO_0009277; GO_0071944 821 YDL025C RTK1 Putative protein kinase, potentially phosphorylated by Cdc28p Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress 822 YDL025W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the ORF YDL025C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 823 YDL026W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 824 YDL027C MRX9 Protein that associates with mitochondrial ribosome Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene C HDA; HDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 825 YDL028C MPS1 Dual-specificity kinase Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p C IDA; IDA condensed nuclear chromosome kinetochore; spindle pole body GO_0000778; GO_0005816 826 YDL029W ARP2 Essential component of the Arp2/3 complex Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants C IDA; IDA mitochondrion; Arp2/3 protein complex GO_0005739; GO_0005885 827 YDL030W PRP9 Subunit of the SF3a splicing factor complex Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex C IDA U2-type prespliceosome GO_0071004 828 YDL031W DBP10 Putative ATP-dependent RNA helicase of the DEAD-box protein family Putative ATP-dependent RNA helicase of the DEAD-box protein family; constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis C IDA; IPI nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 829 YDL032W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene 830 YDL033C SLM3 tRNA-specific 2-thiouridylase tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) C HDA mitochondrion GO_0005739 831 YDL034W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene 832 YDL035C GPR1 Plasma membrane G protein coupled receptor (GPCR) Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis C IDA plasma membrane GO_0005886 833 YDL036C PUS9 Mitochondrial tRNA:pseudouridine synthase Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication C HDA; IMP; HDA cytoplasm; mitochondrion; nucleus GO_0005737; GO_0005739; GO_0005634 834 YDL037C BSC1 Protein of unconfirmed function Protein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression 835 YDL039C PRM7 Pheromone-regulated protein Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements 836 YDL040C NAT1 Subunit of protein N-terminal acetyltransferase NatA Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing C HDA; IDA; IDA mitochondrion; cytosolic ribosome; NatA complex GO_0005739; GO_0022626; GO_0031415 837 YDL041W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C 838 YDL042C SIR2 Conserved NAD+ dependent histone deacetylase of the Sirtuin family Conserved NAD+ dependent histone deacetylase of the Sirtuin family; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication; functions as a regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy; SIR2 has a paralog, HST1, that arose from the whole genome duplication C IDA; IDA; IMP; IDA; IDA; IDA RENT complex; nuclear telomeric heterochromatin; nuclear chromosome, telomeric region; nuclear heterochromatin; chromatin silencing complex; nucleolus GO_0030869; GO_0005724; GO_0000784; GO_0005720; GO_0005677; GO_0005730 839 YDL043C PRP11 Subunit of the SF3a splicing factor complex Subunit of the SF3a splicing factor complex; required for spliceosome assembly C IDA U2-type prespliceosome GO_0071004 840 YDL044C MTF2 Mitochondrial protein that interacts with mitochondrial RNA polymerase Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription C HDA mitochondrion GO_0005739 841 YDL045C FAD1 Flavin adenine dinucleotide (FAD) synthetase Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin C IDA cytoplasm GO_0005737 842 YDL045W-A MRP10 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria C IDA mitochondrial small ribosomal subunit GO_0005763 843 YDL046W NPC2 Sterol transport protein and functional homolog of human NPC2/He1 Functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes C HDA; IDA fungal-type vacuole; fungal-type vacuole lumen GO_0000324; GO_0000328 844 YDL047W SIT4 Ceramide-activated, type 2A-related serine-threonine phosphatase Type 2A-related serine-threonine phosphatase; functions in the G1/S transition of the mitotic cycle; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6 C IDA; IDA; HDA protein phosphatase type 2A complex; cytoplasm; nucleus GO_0000159; GO_0005737; GO_0005634 845 YDL048C STP4 Protein containing a Kruppel-type zinc-finger domain Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication C HDA; HDA; HDA nucleus; cytoplasm; mitochondrion GO_0005634; GO_0005737; GO_0005739 846 YDL049C KNH1 Protein with similarity to Kre9p Protein with similarity to Kre9p; Kre9p is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance C HDA; IDA fungal-type vacuole; extracellular region GO_0000324; GO_0005576 847 YDL050C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 848 YDL051W LHP1 RNA binding protein required for maturation of tRNA and U6 snRNA RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen C IDA; IDA; IDA nucleolus; nucleoplasm; nucleus GO_0005730; GO_0005654; GO_0005634 849 YDL052C SLC1 1-acyl-sn-glycerol-3-phosphate acyltransferase 1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes C HDA; IDA endoplasmic reticulum; lipid particle GO_0005783; GO_0005811 850 YDL053C PBP4 Pbp1p binding protein Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; IDA cytoplasm; nucleus; cytoplasmic stress granule GO_0005737; GO_0005634; GO_0010494 851 YDL054C MCH1 Protein with similarity to mammalian monocarboxylate permeases Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport C HDA fungal-type vacuole membrane GO_0000329 852 YDL055C PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase) GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase); synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure C HDA cytoplasm GO_0005737 853 YDL056W MBP1 Transcription factor Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes C HDA; IDA nucleus; MBF transcription complex GO_0005634; GO_0030907 854 YDL057W YDL057W Putative protein of unknown function Putative protein of unknown function; YDL057W is not an essential gene 855 YDL058W USO1 Essential protein involved in vesicle-mediated ER to Golgi transport Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex C IDA; IDA ER to Golgi transport vesicle membrane; Golgi membrane GO_0012507; GO_0000139 856 YDL059C RAD59 Protein involved DNA double-strand break repair Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 857 YDL060W TSR1 Protein required for processing of 20S pre-rRNA in the cytoplasm Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA; HDA; IDA; IDA cytoplasm; 90S preribosome; nucleus; preribosome, small subunit precursor; nucleolus GO_0005737; GO_0030686; GO_0005634; GO_0030688; GO_0005730 858 YDL061C RPS29B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 859 YDL062W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential 860 YDL063C SYO1 Transport adaptor or symportin Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 861 YDL064W UBC9 SUMO-conjugating enzyme involved in the Smt3p conjugation pathway SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) C IDA; IDA condensed nuclear chromosome; nucleus GO_0000794; GO_0005634 862 YDL065C PEX19 Chaperone and import receptor for newly-synthesized class I PMPs Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning C IDA; HDA; IDA; IDA peroxisomal membrane; cytosol; endoplasmic reticulum; Pex17p-Pex14p docking complex GO_0005778; GO_0005829; GO_0005783; GO_1990415 863 YDL066W IDP1 Mitochondrial NADP-specific isocitrate dehydrogenase Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes C IDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 864 YDL067C COX9 Subunit VIIa of cytochrome c oxidase (Complex IV) Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 865 YDL068W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 866 YDL069C CBS1 Mitochondrial translational activator of the COB mRNA Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader C IPI; HDA; IDA mitochondrial ribosome; mitochondrion; extrinsic component of mitochondrial inner membrane GO_0005761; GO_0005739; GO_0031314 867 YDL070W BDF2 Protein involved in transcription initiation Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication C HDA cell periphery GO_0071944 868 YDL071C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W 869 YDL072C YET3 Protein of unknown function Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress C IDA endoplasmic reticulum GO_0005783 870 YDL073W AHK1 Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway Putative protein of unknown function; YDL073W is not an essential gene 871 YDL074C BRE1 E3 ubiquitin ligase E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing C IDA nuclear chromatin GO_0000790 872 YDL075W RPL31A Ribosomal 60S subunit protein L31A Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 873 YDL076C RXT3 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA nucleus; Rpd3L complex; Rpd3L-Expanded complex GO_0005634; GO_0033698; GO_0070210 874 YDL077C VAM6 Guanine nucleotide exchange factor for the GTPase Gtr1p Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p, facilitating tethering and promoting several membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking C IDA; IDA; IDA; IPI vacuole-mitochondrion membrane contact site; fungal-type vacuole membrane; fungal-type vacuole; HOPS complex GO_1990816; GO_0000329; GO_0000324; GO_0030897 875 YDL078C MDH3 Peroxisomal malate dehydrogenase Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle C IDA; IDA; IDA peroxisome; peroxisomal importomer complex; peroxisomal matrix GO_0005777; GO_1990429; GO_0005782 876 YDL079C MRK1 Glycogen synthase kinase 3 (GSK-3) homolog Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication 877 YDL080C THI3 Regulatory protein that binds Pdc2p and Thi2p transcription factors Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected C HDA nucleus GO_0005634 878 YDL081C RPP1A Ribosomal stalk protein P1 alpha Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component C IDA cytosolic large ribosomal subunit GO_0022625 879 YDL082W RPL13A Ribosomal 60S subunit protein L13A Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 880 YDL083C RPS16B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication 881 YDL084W SUB2 Component of the TREX complex required for nuclear mRNA export Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; IDA; IDA; HDA nucleus; transcription export complex; chromosome, telomeric region; cytoplasm GO_0005634; GO_0000346; GO_0000781; GO_0005737 882 YDL085C-A YDL085C-A Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 883 YDL085W NDE2 Mitochondrial external NADH dehydrogenase Mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 884 YDL086C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps uncharacterized ORF YDL086W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 885 YDL086W YDL086W Putative carboxymethylenebutenolidase Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 886 YDL087C LUC7 Essential protein associated with the U1 snRNP complex Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia C IDA; IMP; IDA; IDA nucleus; U1 snRNP; U2-type prespliceosome; cytosol GO_0005634; GO_0005685; GO_0071004; GO_0005829 887 YDL088C ASM4 FG-nucleoporin component of central core of nuclear pore complex (NPC) FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication C IDA; IDA nuclear pore; nuclear pore central transport channel GO_0005643; GO_0044613 888 YDL089W NUR1 Protein involved in regulation of mitotic exit Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate C HDA nuclear periphery GO_0034399 889 YDL090C RAM1 Beta subunit of the CAAX farnesyltransferase (FTase) Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit C IDA protein farnesyltransferase complex GO_0005965 890 YDL091C UBX3 Clathrin-coated vesicle component, regulator of endocytosis Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10 C HDA; IDA; HDA; IDA cytoplasm; clathrin-coated vesicle; endoplasmic reticulum; Dsc E3 ubiquitin ligase complex GO_0005737; GO_0030136; GO_0005783; GO_0044695 891 YDL092W SRP14 Signal recognition particle (SRP) subunit Signal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 C IDA signal recognition particle, endoplasmic reticulum targeting GO_0005786 892 YDL093W PMT5 Protein O-mannosyltransferase Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals C HDA; IDA; IDA endoplasmic reticulum; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex GO_0005783; GO_0097584; GO_0097585 893 YDL094C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential 894 YDL095W PMT1 Protein O-mannosyltransferase of the ER membrane Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen C IDA; HDA; IDA dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex; endoplasmic reticulum; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex GO_0097582; GO_0005783; GO_0097583 895 YDL096C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential 896 YDL097C RPN6 Essential, non-ATPase regulatory subunit of the 26S proteasome lid Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress C IDA; IDA proteasome storage granule; proteasome regulatory particle, lid subcomplex GO_0034515; GO_0008541 897 YDL098C SNU23 Component of the U4/U6.U5 snRNP complex Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome C IDA U4/U6 x U5 tri-snRNP complex GO_0046540 898 YDL099W BUG1 Cis-golgi localized protein involved in ER to Golgi transport Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes C IDA; HDA cis-Golgi network; cytoplasm GO_0005801; GO_0005737 899 YDL100C GET3 Guanine nucleotide exchange factor for Gpa1p Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of the GET complex, which is involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into the ER membrane under non-stress conditions; has low-level ATPase activity; protein abundance increases in response to DNA replication stress C HDA; HDA; IPI endoplasmic reticulum; cytosol; GET complex GO_0005783; GO_0005829; GO_0043529 900 YDL101C DUN1 Cell-cycle checkpoint S/T protein kinase Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair C HDA nucleus GO_0005634 901 YDL102W POL3 Catalytic subunit of DNA polymerase delta Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) C IDA replication fork GO_0005657 902 YDL103C QRI1 UDP-N-acetylglucosamine pyrophosphorylase UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 903 YDL104C QRI7 Protein involved in threonylcarbamoyl adenosine biosynthesis Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex C HDA mitochondrion GO_0005739 904 YDL105W NSE4 Component of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair C HDA; IDA nucleus; Smc5-Smc6 complex GO_0005634; GO_0030915 905 YDL106C PHO2 Homeobox transcription factor Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 906 YDL107W MSS2 Peripherally bound inner membrane protein of the mitochondrial matrix Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p C IDA; IDA extrinsic component of mitochondrial inner membrane; mitochondrial matrix GO_0031314; GO_0005759 907 YDL108W KIN28 Serine/threonine protein kinase, subunit of transcription factor TFIIH Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA TFIIK complex; holo TFIIH complex; cytosol; nucleus GO_0070985; GO_0005675; GO_0005829; GO_0005634 908 YDL109C YDL109C Putative lipase Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication 909 YDL110C TMA17 ATPase dedicated chaperone that adapts proteasome assembly to stress ATPase dedicated chaperone that adapts proteasome assembly to stress; Tma17p is induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion C HDA; IDA; HDA cytoplasm; ribosome; nucleus GO_0005737; GO_0005840; GO_0005634 910 YDL111C RRP42 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) C IDA; IDA nuclear exosome (RNase complex); cytoplasmic exosome (RNase complex) GO_0000176; GO_0000177 911 YDL112W TRM3 2'-O-ribose methyltransferase 2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs C HDA cytoplasm GO_0005737 912 YDL113C ATG20 Sorting nexin family member Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate C IDA; IDA; IDA pre-autophagosomal structure; endosome; extrinsic component of membrane GO_0000407; GO_0005768; GO_0019898 913 YDL114W YDL114W Putative short-chain dehydrogenase/reductase Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene 914 YDL114W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified based on homology to hemiascomycetous yeasts 915 YDL115C IWR1 RNA polymerase II transport factor, conserved from yeast to humans RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; IDA; IPI nucleus; cytoplasm; DNA-directed RNA polymerase II, holoenzyme GO_0005634; GO_0005737; GO_0016591 916 YDL116W NUP84 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 C IDA; IDA nuclear pore outer ring; nuclear pore GO_0031080; GO_0005643 917 YDL117W CYK3 SH3-domain protein located in the bud neck and cytokinetic actin ring SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants C IPI; IDA; HDA; HDA; IDA HICS complex; cellular bud neck; cytoplasm; nucleus; cellular bud neck contractile ring GO_0044697; GO_0005935; GO_0005737; GO_0005634; GO_0000142 918 YDL118W Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein 919 YDL119C HEM25 Mitochondrial glycine transporter Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria C IDA mitochondrion GO_0005739 920 YDL120W YFH1 Mitochondrial matrix iron chaperone Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia C IDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 921 YDL121C YDL121C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein C IDA endoplasmic reticulum GO_0005783 922 YDL122W UBP1 Ubiquitin-specific protease Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains C IDA; IDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 923 YDL123W SNA4 Protein of unknown function Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated C HDA; IDA fungal-type vacuole membrane; fungal-type vacuole lumen GO_0000329; GO_0000328 924 YDL124W YDL124W NADPH-dependent alpha-keto amide reductase NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA plasma membrane; cytoplasm; nucleus GO_0005886; GO_0005737; GO_0005634 925 YDL125C HNT1 Adenosine 5'-monophosphoramidase Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 926 YDL126C CDC48 AAA ATPase AAA ATPase; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing, in macroautophagy, PMN, RAD, ribophagy, homotypic ER membrane fusion, disassembly of Met30p from SCF complex; functional ortholog of human p97/VCP C IDA; IDA; IDA; IDA; HDA; IDA; HDA; HDA; IDA; IDA; IDA cytosolic large ribosomal subunit; cytosol; endoplasmic reticulum membrane; VCP-NPL4-UFD1 AAA ATPase complex; nucleus; Cdc48p-Npl4p-Vms1p AAA ATPase complex; mitochondrion; mating projection tip; RQC complex; Doa10p ubiquitin ligase complex; Hrd1p ubiquitin ligase ERAD-L complex GO_0022625; GO_0005829; GO_0005789; GO_0034098; GO_0005634; GO_0036266; GO_0005739; GO_0043332; GO_1990112; GO_0000837; GO_0000839 927 YDL127W PCL2 Cyclin, interacts with cyclin-dependent kinase Pho85p Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IPI cellular bud neck; cellular bud tip; nucleus; incipient cellular bud site; cyclin-dependent protein kinase holoenzyme complex GO_0005935; GO_0005934; GO_0005634; GO_0000131; GO_0000307 928 YDL128W VCX1 Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter C IDA; IDA fungal-type vacuole membrane; fungal-type vacuole GO_0000329; GO_0000324 929 YDL129W YDL129W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 930 YDL130W RPP1B Ribosomal protein P1 beta Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component C HDA; IDA fungal-type vacuole; cytosolic large ribosomal subunit GO_0000324; GO_0022625 931 YDL130W-A STF1 Protein involved in regulation of the mitochondrial F1F0-ATP synthase Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 932 YDL131W LYS21 Homocitrate synthase isozyme Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication C HDA nucleus GO_0005634 933 YDL132W CDC53 Cullin Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation C IDA SCF ubiquitin ligase complex GO_0019005 934 YDL133C-A RPL41B Ribosomal 60S subunit protein L41B Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 935 YDL133W SRF1 Regulator of phospholipase D (Spo14p) Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients C HDA; HDA cell periphery; fungal-type vacuole GO_0071944; GO_0000324 936 YDL134C PPH21 Catalytic subunit of protein phosphatase 2A, PP2A Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication C IDA; HDA; HDA; IDA protein phosphatase type 2A complex; cytoplasm; nucleus; cytoplasmic stress granule GO_0000159; GO_0005737; GO_0005634; GO_0010494 937 YDL135C RDI1 Rho GDP dissociation inhibitor Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; IDA plasma membrane; cytosol; cellular bud neck; cellular bud tip GO_0005886; GO_0005829; GO_0005935; GO_0005934 938 YDL136W RPL35B Ribosomal 60S subunit protein L35B Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication C IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 939 YDL137W ARF2 ADP-ribosylation factor ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication C HDA; HDA cytosol; Golgi apparatus GO_0005829; GO_0005794 940 YDL138W RGT2 Plasma membrane high glucose sensor that regulates glucose transport Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication C IMP; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 941 YDL139C SCM3 Nonhistone component of centromeric chromatin Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP C IDA; HDA; HDA condensed nuclear chromosome, centromeric region; nucleus; cytoplasm GO_0000780; GO_0005634; GO_0005737 942 YDL140C RPO21 RNA polymerase II largest subunit B220 RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime C HDA; IDA; HDA; IDA mitochondrion; cytoplasmic stress granule; nucleus; DNA-directed RNA polymerase II, core complex GO_0005739; GO_0010494; GO_0005634; GO_0005665 943 YDL141W BPL1 Biotin:apoprotein ligase Biotin:apoprotein ligase; covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 944 YDL142C CRD1 Cardiolipin synthase Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis C HDA mitochondrion GO_0005739 945 YDL143W CCT4 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo C IPI chaperonin-containing T-complex GO_0005832 946 YDL144C YDL144C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 947 YDL145C COP1 Alpha subunit of COPI vesicle coatomer complex Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway C IMP COPI vesicle coat GO_0030126 948 YDL146W LDB17 Protein involved in the regulation of endocytosis Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck C HDA; HDA; IDA; HDA cellular bud neck; cytoplasm; actin cortical patch; cellular bud GO_0005935; GO_0005737; GO_0030479; GO_0005933 949 YDL147W RPN5 Subunit of the CSN and 26S proteasome lid complexes Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN C IDA; IDA; IDA; IDA proteasome complex; COP9 signalosome; proteasome regulatory particle, lid subcomplex; proteasome storage granule GO_0000502; GO_0008180; GO_0008541; GO_0034515 950 YDL148C NOP14 Nucleolar protein Nucleolar protein; forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA C IDA; IDA; HDA; HDA; IPI nucleolus; small-subunit processome; mitochondrion; 90S preribosome; Noc4p-Nop14p complex GO_0005730; GO_0032040; GO_0005739; GO_0030686; GO_0030692 951 YDL149W ATG9 Transmembrane protein involved in forming Cvt and autophagic vesicles Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS C IDA; IDA; IDA; IDA; IDA cytoplasmic vesicle membrane; pre-autophagosomal structure; integral component of membrane; NA; mitochondrion GO_0030659; GO_0000407; GO_0016021; GO_0061908; GO_0005739 952 YDL150W RPC53 RNA polymerase III subunit C53 RNA polymerase III subunit C53 C IDA DNA-directed RNA polymerase III complex GO_0005666 953 YDL151C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay 954 YDL152W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome 955 YDL153C SAS10 Subunit of U3-containing Small Subunit (SSU) processome complex Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene C IDA; IDA; IDA small-subunit processome; nucleus; nucleolus GO_0032040; GO_0005634; GO_0005730 956 YDL154W MSH5 Protein of the MutS family Protein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans C HDA nucleus GO_0005634 957 YDL155W CLB3 B-type cyclin involved in cell cycle progression B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 958 YDL156W CMR1 Nuclear protein with a role in protein quality control DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS C HDA; IDA; HDA; IDA cytoplasm; nuclear chromatin; nucleus; nuclear periphery GO_0005737; GO_0000790; GO_0005634; GO_0034399 959 YDL157C YDL157C Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 960 YDL158C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 961 YDL159C-B Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 962 YDL159W STE7 Signal transducing MAP kinase kinase Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway C IDA; IDA mating projection tip; cytoplasm GO_0043332; GO_0005737 963 YDL159W-A YDL159W-A Putative protein of unknown function Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species 964 YDL160C DHH1 Cytoplasmic DEAD-box helicase, stimulates mRNA decapping Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, role in translational repression, mRNA decay, and processing body dynamics; may have a role in mRNA export; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; IDA; HDA cytoplasmic side of membrane; cytoplasmic stress granule; P-body; cytoplasm GO_0098562; GO_0010494; GO_0000932; GO_0005737 965 YDL160C-A MHF2 Component of the heterotetrameric MHF histone-fold complex Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 C IDA FANCM-MHF complex GO_0071821 966 YDL161W ENT1 Epsin-like protein involved in endocytosis and actin patch assembly Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication C HDA; HDA; IDA; HDA; HDA cellular bud neck; cytoplasm; early endosome; mating projection tip; cellular bud tip GO_0005935; GO_0005737; GO_0005769; GO_0043332; GO_0005934 967 YDL162C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly 968 YDL163W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase 969 YDL164C CDC9 DNA ligase I found in nucleus and mitochondria DNA ligase found in the nucleus and mitochondria; an essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination, C IDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 970 YDL165W CDC36 Component of the CCR4-NOT core complex, involved in mRNA decapping Component of the CCR4-NOT core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor C IDA; IPI; IDA nucleus; CCR4-NOT core complex; cytoplasm GO_0005634; GO_0030015; GO_0005737 971 YDL166C FAP7 Essential NTPase required for small ribosome subunit synthesis Essential NTPase required for small ribosome subunit synthesis; mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D; depletion leads to accumulation of pre-40S ribosomes in 80S-like ribosomes C IMP; IDA; IDA cytoplasm; nucleus; cytoplasmic stress granule GO_0005737; GO_0005634; GO_0010494 972 YDL167C NRP1 Putative RNA binding protein of unknown function Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis C IDA; HDA cytoplasmic stress granule; cytoplasm GO_0010494; GO_0005737 973 YDL168W SFA1 Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 974 YDL169C UGX2 Protein of unknown function Protein of unknown function; transcript accumulates in response to any combination of stress conditions 975 YDL170W UGA3 Transcriptional activator for GABA-dependent induction of GABA genes Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4 C IDA nucleus GO_0005634 976 YDL171C GLT1 NAD(+)-dependent glutamate synthase (GOGAT) NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions C HDA mitochondrion GO_0005739 977 YDL172C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 978 YDL173W PAR32 Low complexity protein Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene C HDA cytoplasm GO_0005737 979 YDL174C DLD1 Major mitochondrial D-lactate dehydrogenase D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 980 YDL175C AIR2 RNA-binding subunit of the TRAMP nuclear RNA surveillance complex RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome C IDA; IDA TRAMP complex; nucleolus GO_0031499; GO_0005730 981 YDL176W YDL176W Protein of unknown function Protein of unknown function; predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene 982 YDL177C YDL177C Putative protein of unknown function Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene 983 YDL178W DLD2 D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase D-lactate dehydrogenase; located in the mitochondrial matrix C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 984 YDL179W PCL9 Cyclin Cyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication C IPI; IDA; IDA cyclin-dependent protein kinase holoenzyme complex; cellular bud neck; incipient cellular bud site GO_0000307; GO_0005935; GO_0000131 985 YDL180W YDL180W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole C HDA; HDA fungal-type vacuole; fungal-type vacuole membrane GO_0000324; GO_0000329 986 YDL181W INH1 Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 987 YDL182W LYS20 Homocitrate synthase isozyme and functions in DNA repair Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication C HDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 988 YDL183C YDL183C Protein that may form an active mitochondrial KHE system Protein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system C IDA integral component of mitochondrial inner membrane GO_0031305 989 YDL184C RPL41A Ribosomal 60S subunit protein L41A Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 990 YDL185C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDL185W; identified by homology with hemiascomycetous yeast species 991 YDL185W VMA1 Subunit A of the V1 peripheral membrane domain of V-ATPase Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner C HDA; IDA fungal-type vacuole membrane; vacuolar proton-transporting V-type ATPase, V1 domain GO_0000329; GO_0000221 992 YDL186W YDL186W Putative protein of unknown function Putative protein of unknown function; YDL186W is not an essential gene 993 YDL187C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 994 YDL188C PPH22 Catalytic subunit of protein phosphatase 2A, PP2A Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication C IDA; IDA nuclear periphery; condensed nuclear chromosome, centromeric region GO_0034399; GO_0000780 995 YDL189W RBS1 Protein involved in assembly of the RNA polymerase III (Pol III) comp Protein of unknown function; identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 996 YDL190C UFD2 Ubiquitin chain assembly factor (E4) Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 997 YDL191W RPL35A Ribosomal 60S subunit protein L35A Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication C IDA; IDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 998 YDL192W ARF1 ADP-ribosylation factor ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication C HDA; HDA cytosol; Golgi apparatus GO_0005829; GO_0005794 999 YDL193W NUS1 Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1 Putative prenyltransferase; required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY C IDA; IDA; HDA; IDA lipid particle; dehydrodolichyl diphosphate synthase complex; nuclear envelope; endoplasmic reticulum GO_0005811; GO_1904423; GO_0005635; GO_0005783 1000 YDL194W SNF3 Plasma membrane low glucose sensor, regulates glucose transport Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 1001 YDL195W SEC31 Component of the Sec13p-Sec31p complex of the COPII vesicle coat Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance C IDA; HDA COPII vesicle coat; mating projection tip GO_0030127; GO_0043332 1002 YDL196W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 1003 YDL197C ASF2 Anti-silencing protein Anti-silencing protein; causes derepression of silent loci when overexpressed C HDA; IDA nucleus; nuclear chromosome, telomeric region GO_0005634; GO_0000784 1004 YDL198C GGC1 Mitochondrial GTP/GDP transporter Mitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family C HDA mitochondrion GO_0005739 1005 YDL199C YDL199C Putative transporter Putative transporter; member of the sugar porter family C HDA fungal-type vacuole membrane GO_0000329 1006 YDL200C MGT1 DNA repair methyltransferase (6-O-methylguanine-DNA methylase) DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage 1007 YDL201W TRM8 Catalytic subunit of a tRNA methyltransferase complex Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p C HDA; IDA nucleus; tRNA methyltransferase complex GO_0005634; GO_0043527 1008 YDL202W MRPL11 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2 C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1009 YDL203C ACK1 Protein that functions in the cell wall integrity pathway Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria C HDA mitochondrion GO_0005739 1010 YDL204W RTN2 Reticulon protein Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication C HDA; HDA; IDA; HDA; HDA; IDA cytoplasm; cell periphery; cortical endoplasmic reticulum; endoplasmic reticulum; nucleus; endoplasmic reticulum tubular network GO_0005737; GO_0071944; GO_0032541; GO_0005783; GO_0005634; GO_0071782 1011 YDL205C HEM3 Porphobilinogen deaminase Porphobilinogen deaminase; catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1012 YDL206W YDL206W Putative protein of unknown function Putative protein of unknown function; YDL206W is not an essential protein C HDA fungal-type vacuole membrane GO_0000329 1013 YDL207W GLE1 Cytoplasmic nucleoporin required for polyadenylated mRNA export Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination C IDA; IDA; IDA; HDA nuclear pore cytoplasmic filaments; cytoplasm; nuclear pore; mitochondrion GO_0044614; GO_0005737; GO_0005643; GO_0005739 1014 YDL208W NHP2 Protein related to mammalian high mobility group (HMG) proteins Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing C IPI box H/ACA snoRNP complex GO_0031429 1015 YDL209C CWC2 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 C IPI; IDA; IDA Prp19 complex; U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome GO_0000974; GO_0071006; GO_0071007 1016 YDL210W UGA4 GABA (gamma-aminobutyrate) permease GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane C IDA fungal-type vacuole membrane GO_0000329 1017 YDL211C YDL211C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 1018 YDL212W SHR3 Endoplasmic reticulum packaging chaperone Endoplasmic reticulum packaging chaperone; required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface C IDA; HDA; HDA integral component of endoplasmic reticulum membrane; endoplasmic reticulum; mating projection tip GO_0030176; GO_0005783; GO_0043332 1019 YDL213C NOP6 rRNA-binding protein required for 40S ribosomal subunit biogenesis rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes C IDA; HDA; IDA; IDA 90S preribosome; nucleolus; preribosome, large subunit precursor; preribosome, small subunit precursor GO_0030686; GO_0005730; GO_0030687; GO_0030688 1020 YDL214C PRR2 Serine/threonine protein kinase Serine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication 1021 YDL215C GDH2 NAD(+)-dependent glutamate dehydrogenase NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis C HDA mitochondrion GO_0005739 1022 YDL216C RRI1 Catalytic subunit of the COP9 signalosome (CSN) complex Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling C IDA COP9 signalosome GO_0008180 1023 YDL217C TIM22 Essential core component of the mitochondrial TIM22 complex Essential core component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported C IDA; HDA mitochondrial inner membrane protein insertion complex; mitochondrion GO_0042721; GO_0005739 1024 YDL218W YDL218W Putative protein of unknown function Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin C HDA cytoplasm GO_0005737 1025 YDL219W DTD1 D-Tyr-tRNA(Tyr) deacylase D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes C HDA cytoplasm GO_0005737 1026 YDL220C CDC13 Single stranded DNA-binding protein found at TG1-3 telomere G-tails Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle C IDA; IPI nuclear chromosome, telomeric region; CST complex GO_0000784; GO_1990879 1027 YDL221W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 1028 YDL222C FMP45 Integral membrane protein localized to mitochondria Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication C HDA; HDA; HDA; IDA mitochondrion; plasma membrane; cell periphery; cell cortex GO_0005739; GO_0005886; GO_0071944; GO_0005938 1029 YDL223C HBT1 Shmoo tip protein, substrate of Hub1p ubiquitin-like protein Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication C IDA; HDA mating projection; plasma membrane GO_0005937; GO_0005886 1030 YDL224C WHI4 Putative RNA binding protein Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1031 YDL225W SHS1 Component of the septin ring that is required for cytokinesis Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress C HDA; HDA; IDA; IPI mating projection tip; prospore membrane; cellular bud neck septin ring; cellular bud neck GO_0043332; GO_0005628; GO_0000144; GO_0005935 1032 YDL226C GCS1 ADP-ribosylation factor GTPase activating protein (ARF GAP) ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication C IPI; HDA; IPI; IDA; IDA cytoskeleton; cytosol; endoplasmic reticulum-Golgi intermediate compartment; trans-Golgi network; endosome GO_0005856; GO_0005829; GO_0005793; GO_0005802; GO_0005768 1033 YDL227C HO Site-specific endonuclease Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p C HDA nucleus GO_0005634 1034 YDL228C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene SSB1 1035 YDL229W SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication C IDA; IDA; HDA polysome; cytoplasm; plasma membrane GO_0005844; GO_0005737; GO_0005886 1036 YDL230W PTP1 Phosphotyrosine-specific protein phosphatase Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 1037 YDL231C BRE4 Zinc finger protein containing five transmembrane domains Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport C HDA cell periphery GO_0071944 1038 YDL232W OST4 Subunit of the oligosaccharyltransferase complex of the ER lumen Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex C IPI; HDA; IMP oligosaccharyltransferase complex; mitochondrion; endoplasmic reticulum membrane GO_0008250; GO_0005739; GO_0005789 1039 YDL233W MFG1 Regulator of filamentous growth Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1040 YDL234C GYP7 GTPase-activating protein for yeast Rab family members GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 1041 YDL235C YPD1 Osmotic stress-responsive phosphorelay intermediate sensor protein Osmotic stress-responsive phosphorelay intermediate sensor protein; phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1042 YDL236W PHO13 Conserved phosphatase acting as a metabolite repair enzyme Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1043 YDL237W AIM6 Protein of unknown function Putative protein of unknown function; required for respiratory growth; YDL237W is not an essential gene 1044 YDL238C GUD1 Guanine deaminase Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures C HDA cytoplasm GO_0005737 1045 YDL239C ADY3 Protein required for spore wall formation Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication C IDA; IDA; IPI prospore membrane leading edge; prospore membrane; spindle pole body GO_0070056; GO_0005628; GO_0005816 1046 YDL240C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species 1047 YDL240W LRG1 GTPase-activating protein (GAP) GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains C HDA; IDA; IDA; IDA; IDA; HDA; IDA cytoplasm; incipient cellular bud site; site of polarized growth; cellular bud tip; cellular bud neck; mitochondrion; mating projection tip GO_0005737; GO_0000131; GO_0030427; GO_0005934; GO_0005935; GO_0005739; GO_0043332 1048 YDL241W YDL241W Putative protein of unknown function Putative protein of unknown function; YDL241W is not an essential gene C HDA endoplasmic reticulum GO_0005783 1049 YDL242W YDL242W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1050 YDL243C AAD4 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 1051 YDL244W THI13 Protein involved in synthesis of the thiamine precursor HMP Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP 1052 YDL245C HXT15 Putative transmembrane polyol transporter Protein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose C HDA cell periphery GO_0071944 1053 YDL246C SOR2 Sorbitol dehydrogenase Protein of unknown function; protein sequence is 99% identical to the Sor1p sorbitol dehydrogenase; computational analysis of large-scale protein-protein interaction data also suggests a role in fructose or mannose metabolism 1054 YDL247W MPH2 Alpha-glucoside permease Alpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication C HDA cell periphery GO_0071944 1055 YDL247W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species 1056 YDL248W COS7 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA mitochondrion; fungal-type vacuole GO_0005739; GO_0000324 1057 YDR001C NTH1 Neutral trehalase, degrades trehalose Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1058 YDR002W YRB1 Ran GTPase binding protein Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1059 YDR003W RCR2 Vacuolar protein Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication C HDA; IDA; HDA; IDA fungal-type vacuole membrane; vesicle; cytoplasm; fungal-type vacuole GO_0000329; GO_0031982; GO_0005737; GO_0000324 1060 YDR003W-A YDR003W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 1061 YDR004W RAD57 Protein that stimulates strand exchange Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p C IDA; IPI Rhp55-Rhp57 complex; nucleus GO_0033062; GO_0005634 1062 YDR005C MAF1 Highly conserved negative regulator of RNA polymerase III Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions C IDA; IDA; IDA nucleus; cytoplasm; nucleolus GO_0005634; GO_0005737; GO_0005730 1063 YDR006C SOK1 Protein of unknown function Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 C IDA nucleus GO_0005634 1064 YDR007W TRP1 Phosphoribosylanthranilate isomerase Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) C HDA cytoplasm GO_0005737 1065 YDR008C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps TRP1/YDR007W on opposite strand 1066 YDR009W GAL3 Transcriptional regulator Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1067 YDR010C YDR010C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1068 YDR011W SNQ2 Plasma membrane ATP-binding cassette (ABC) transporter Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species C HDA; HDA; IDA cell periphery; mitochondrion; plasma membrane GO_0071944; GO_0005739; GO_0005886 1069 YDR012W RPL4B Ribosomal 60S subunit protein L4B Ribosomal 60S subunit protein L4B; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4B has a paralog, RPL4A, that arose from the whole genome duplication C HDA; IDA cytoplasm; cytosolic large ribosomal subunit GO_0005737; GO_0022625 1070 YDR013W PSF1 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery C IDA; IPI; IMP; IDA CMG complex; GINS complex; DNA replication preinitiation complex; replication fork protection complex GO_0071162; GO_0000811; GO_0031261; GO_0031298 1071 YDR014W RAD61 Subunit of a complex that inhibits sister chromatid cohesion Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin C IDA nuclear cohesin complex GO_0000798 1072 YDR014W-A HED1 Meiosis-specific protein Meiosis-specific protein; down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; prevents the recruitment of Rad54p to site-specific DNA double-strand breaks in vivo; early meiotic gene, transcribed specifically during meiotic prophase C IDA condensed nuclear chromosome GO_0000794 1073 YDR015C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A 1074 YDR016C DAD1 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 1075 YDR017C KCS1 Inositol hexakisphosphate and inositol heptakisphosphate kinase Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7 C HDA cytoplasm GO_0005737 1076 YDR018C YDR018C Probable membrane protein with three predicted transmembrane domains Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication C HDA; HDA nucleus; endoplasmic reticulum GO_0005634; GO_0005783 1077 YDR019C GCV1 T subunit of the mitochondrial glycine decarboxylase complex T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm C HDA mitochondrion GO_0005739 1078 YDR020C DAS2 Putative protein of unknown function Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1079 YDR021W FAL1 Nucleolar protein required for maturation of 18S rRNA Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases C IDA; IPI nucleolus; FAL1-SGD1 complex GO_0005730; GO_0097078 1080 YDR022C ATG31 Autophagy-specific protein required for autophagosome formation Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion C IDA; HDA; IDA; HDA pre-autophagosomal structure; cytoplasm; ATG1/ULK1 kinase complex; cytosol GO_0000407; GO_0005737; GO_1990316; GO_0005829 1081 YDR023W SES1 Cytosolic seryl-tRNA synthetase Cytosolic seryl-tRNA synthetase; class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p C IDA; HDA cytoplasmic stress granule; cytoplasm GO_0010494; GO_0005737 1082 YDR024W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin 1083 YDR025W RPS11A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication C IDA; HDA cytosolic small ribosomal subunit; 90S preribosome GO_0022627; GO_0030686 1084 YDR026C NSI1 RNA polymerase I termination factor RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication C IDA; HDA; HDA rDNA heterochromatin; nucleolus; ribosome GO_0033553; GO_0005730; GO_0005840 1085 YDR027C VPS54 Component of the GARP (Golgi-associated retrograde protein) complex Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p C IDA; HDA; IPI Golgi apparatus; mitochondrion; GARP complex GO_0005794; GO_0005739; GO_0000938 1086 YDR028C REG1 Regulatory subunit of type 1 protein phosphatase Glc7p Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 1087 YDR029W YDR029W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1088 YDR030C RAD28 Protein involved in DNA repair Protein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair 1089 YDR031W MIX14 Mitochondrial intermembrane space protein of unknown function Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress C HDA; HDA; IDA; HDA cytoplasm; mitochondrion; mitochondrial intermembrane space; nucleus GO_0005737; GO_0005739; GO_0005758; GO_0005634 1090 YDR032C PST2 Protein with similarity to a family of flavodoxin-like proteins Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication C IDA; HDA; HDA; HDA membrane raft; mitochondrion; plasma membrane; cytoplasm GO_0045121; GO_0005739; GO_0005886; GO_0005737 1091 YDR033W MRH1 Protein that localizes primarily to the plasma membrane Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication C HDA; HDA; HDA endoplasmic reticulum; plasma membrane; mitochondrion GO_0005783; GO_0005886; GO_0005739 1092 YDR034C LYS14 Transcriptional activator involved in regulating lysine biosynthesis Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer C IDA nucleus GO_0005634 1093 YDR034C-A YDR034C-A Putative protein of unknown function Putative protein of unknown function; contained within the solo Ty1 LTR element YDRWdelta7 1094 YDR034C-C YDR034C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1095 YDR034C-D YDR034C-D Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1096 YDR034W-B YDR034W-B Predicted tail-anchored plasma membrane protein Predicted tail-anchored plasma membrane protein; contains conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; YDR034W-B has a paralog, YBR056W-A, that arose from the whole genome duplication C HDA cell periphery GO_0071944 1097 YDR035W ARO3 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 1098 YDR036C EHD3 3-hydroxyisobutyryl-CoA hydrolase 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis C IDA mitochondrion GO_0005739 1099 YDR037W KRS1 Lysyl-tRNA synthetase Lysyl-tRNA synthetase C IDA cytoplasm GO_0005737 1100 YDR038C ENA5 Protein with similarity to P-type ATPase sodium pumps Protein with similarity to P-type ATPase sodium pumps; member of the Na+ efflux ATPase family C HDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 1101 YDR039C ENA2 P-type ATPase sodium pump P-type ATPase sodium pump; involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux C IDA; HDA plasma membrane; mitochondrion GO_0005886; GO_0005739 1102 YDR040C ENA1 P-type ATPase sodium pump P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance C HDA; IDA; HDA cell periphery; plasma membrane; fungal-type vacuole GO_0071944; GO_0005886; GO_0000324 1103 YDR041W RSM10 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1104 YDR042C YDR042C Putative protein of unknown function Putative protein of unknown function; expression is increased in ssu72-ts69 mutant 1105 YDR043C NRG1 Transcriptional repressor Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose C IDA nucleus GO_0005634 1106 YDR044W HEM13 Coproporphyrinogen III oxidase Coproporphyrinogen III oxidase; an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) C IDA cytosol GO_0005829 1107 YDR045C RPC11 RNA polymerase III subunit C11 RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS C IDA DNA-directed RNA polymerase III complex GO_0005666 1108 YDR046C BAP3 Amino acid permease Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; mitochondrion GO_0005783; GO_0005739 1109 YDR047W HEM12 Uroporphyrinogen decarboxylase Uroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1110 YDR048C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF VMS1/YDR049W 1111 YDR049W VMS1 Component of a Cdc48p-complex involved in protein quality control Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans C HDA; IDA; IDA; HDA; IDA mitochondrion; cytosol; endoplasmic reticulum membrane; cytoplasm; Cdc48p-Npl4p-Vms1p AAA ATPase complex GO_0005739; GO_0005829; GO_0005789; GO_0005737; GO_0036266 1112 YDR050C TPI1 Triose phosphate isomerase, abundant glycolytic enzyme Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease C HDA; HDA; HDA mitochondrion; cytoplasm; plasma membrane GO_0005739; GO_0005737; GO_0005886 1113 YDR051C DET1 Acid phosphatase Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1114 YDR052C DBF4 Regulatory subunit of Cdc7p-Dbf4p kinase complex Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; IPI; HDA; HDA chromatin; chromosome, centromeric region; Dbf4-dependent protein kinase complex; cytoplasm; nucleus GO_0000785; GO_0000775; GO_0031431; GO_0005737; GO_0005634 1115 YDR053W Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex 1116 YDR054C CDC34 Ubiquitin-conjugating enzyme (E2) Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress C IDA; IDA; IMP cytoplasm; nucleus; SCF ubiquitin ligase complex GO_0005737; GO_0005634; GO_0019005 1117 YDR055W PST1 Cell wall protein that contains a putative GPI-attachment site Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication C IDA; HDA; IDA; HDA plasma membrane; fungal-type vacuole; fungal-type cell wall; cell periphery GO_0005886; GO_0000324; GO_0009277; GO_0071944 1118 YDR056C EMC10 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein C HDA endoplasmic reticulum GO_0005783 1119 YDR057W YOS9 ER quality-control lectin ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family C IDA; IPI; IDA; HDA endoplasmic reticulum lumen; Hrd1p ubiquitin ligase ERAD-L complex; luminal surveillance complex; endoplasmic reticulum GO_0005788; GO_0000839; GO_0034099; GO_0005783 1120 YDR058C TGL2 Triacylglycerol lipase that is localized to the mitochondria Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli C IDA mitochondrion GO_0005739 1121 YDR059C UBC5 Ubiquitin-conjugating enzyme Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication C IPI proteasome complex GO_0000502 1122 YDR060W MAK21 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein C HDA; IDA preribosome, large subunit precursor; Noc1p-Noc2p complex GO_0030687; GO_0030690 1123 YDR061W YDR061W Protein with similarity to ABC transporter family members Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance C HDA mitochondrion GO_0005739 1124 YDR062W LCB2 Component of serine palmitoyltransferase Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine C HDA; IDA; IMP endoplasmic reticulum; SPOTS complex; serine C-palmitoyltransferase complex GO_0005783; GO_0035339; GO_0017059 1125 YDR063W AIM7 Protein that interacts with Arp2/3 complex Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss C IDA; IDA; HDA cytoplasm; actin cortical patch; nucleus GO_0005737; GO_0030479; GO_0005634 1126 YDR064W RPS13 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 C IDA; HDA cytosolic small ribosomal subunit; 90S preribosome GO_0022627; GO_0030686 1127 YDR065W RRG1 Protein of unknown function Protein of unknown function; required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 1128 YDR066C RTR2 Protein of unknown function Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1129 YDR067C OCA6 Cytoplasmic protein required for replication of Brome mosaic virus Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT C HDA cytoplasm GO_0005737 1130 YDR068W DOS2 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 1131 YDR069C DOA4 Ubiquitin hydrolase Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication C HDA; IPI; HDA; IDA cytosol; proteasome complex; mitochondrion; endosome GO_0005829; GO_0000502; GO_0005739; GO_0005768 1132 YDR070C FMP16 Protein of unknown function Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 1133 YDR071C PAA1 Polyamine acetyltransferase Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication C HDA cytoplasm GO_0005737 1134 YDR072C IPT1 Inositolphosphotransferase Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin 1135 YDR073W SNF11 Subunit of the SWI/SNF chromatin remodeling complex Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions C IDA; IDA; IDA cytosol; nucleus; SWI/SNF complex GO_0005829; GO_0005634; GO_0016514 1136 YDR074W TPS2 Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress C HDA; IPI mitochondrion; alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) GO_0005739; GO_0005946 1137 YDR075W PPH3 Catalytic subunit of protein phosphatase PP4 complex Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes C IDA; IDA; HDA; HDA; IDA protein phosphatase 4 complex; condensed nuclear chromosome; nucleus; cytoplasm; nuclear periphery GO_0030289; GO_0000794; GO_0005634; GO_0005737; GO_0034399 1138 YDR076W RAD55 Protein that stimulates strand exchange Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p C IDA; IPI Rhp55-Rhp57 complex; nucleus GO_0033062; GO_0005634 1139 YDR077W SED1 Major stress-induced structural GPI-cell wall glycoprotein Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication C IDA; HDA; IDA; IDA fungal-type cell wall; cell periphery; ribosome; mitochondrion GO_0009277; GO_0071944; GO_0005840; GO_0005739 1140 YDR078C SHU2 Component of Shu complex (aka PCSS complex) Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments C IPI; IMP Shu complex; site of double-strand break GO_0097196; GO_0035861 1141 YDR079C-A TFB5 Component of RNA polymerase II general transcription factor TFIIH Component of RNA polymerase II general transcription factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; relocalizes to the cytosol in response to hypoxia; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair C IDA; IDA; IPI; IDA nucleus; holo TFIIH complex; core TFIIH complex; cytosol GO_0005634; GO_0005675; GO_0000439; GO_0005829 1142 YDR079W PET100 Chaperone that facilitates the assembly of cytochrome c oxidase Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme C IDA; HDA; IDA integral component of mitochondrial inner membrane; mitochondrion; mitochondrial inner membrane GO_0031305; GO_0005739; GO_0005743 1143 YDR080W VPS41 Subunit of the HOPS endocytic tethering complex Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites C IPI; HDA; HDA HOPS complex; fungal-type vacuole membrane; endosome GO_0030897; GO_0000329; GO_0005768 1144 YDR081C PDC2 Transcription factor for thiamine-regulated genes Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions 1145 YDR082W STN1 Telomere end-binding and capping protein Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1 C IPI CST complex GO_1990879 1146 YDR083W RRP8 Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 C IDA; IDA; IDA; IDA preribosome, small subunit precursor; 90S preribosome; preribosome, large subunit precursor; nucleolus GO_0030688; GO_0030686; GO_0030687; GO_0005730 1147 YDR084C TVP23 Integral membrane protein Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C IDA; HDA integral component of Golgi membrane; cytoplasm GO_0030173; GO_0005737 1148 YDR085C AFR1 Protein required for pheromone-induced projection (shmoo) formation Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication C IDA mating projection base GO_0001400 1149 YDR086C SSS1 Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p); this complex forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p C IDA; IDA; HDA Sec61 translocon complex; Ssh1 translocon complex; endoplasmic reticulum GO_0005784; GO_0071261; GO_0005783 1150 YDR087C RRP1 Essential evolutionarily conserved nucleolar protein Essential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles C IDA; HDA; HDA preribosome, large subunit precursor; nucleolus; nucleus GO_0030687; GO_0005730; GO_0005634 1151 YDR088C SLU7 RNA splicing factor RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain C IDA; IDA U2-type post-spliceosomal complex; spliceosomal complex GO_0071021; GO_0005681 1152 YDR089W VTC5 Novel subunit of the vacuolar transporter chaperone complex Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress C IPI; HDA; IDA vacuolar transporter chaperone complex; fungal-type vacuole membrane; integral component of fungal-type vacuolar membrane GO_0033254; GO_0000329; GO_0071627 1153 YDR090C YDR090C Putative protein of unknown function Putative protein of unknown function 1154 YDR091C RLI1 Essential Fe-S protein Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress C HDA; IDA; IDA; HDA cytoplasm; nucleus; cytosolic ribosome; preribosome, large subunit precursor GO_0005737; GO_0005634; GO_0022626; GO_0030687 1155 YDR092W UBC13 E2 ubiquitin-conjugating enzyme E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus C IDA; IPI; IDA nucleus; ubiquitin conjugating enzyme complex; cytoplasm GO_0005634; GO_0031371; GO_0005737 1156 YDR093W DNF2 Aminophospholipid translocase (flippase) Aminophospholipid translocase (flippase); localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase; DNF2 has a paralog, DNF1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA; HDA plasma membrane; cell periphery; cellular bud neck; mating projection tip membrane; endoplasmic reticulum GO_0005886; GO_0071944; GO_0005935; GO_0070867; GO_0005783 1157 YDR094W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2/YDR093W 1158 YDR095C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1159 YDR096W GIS1 Histone demethylase and transcription factor Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication C HDA; HDA; IDA mitochondrion; nucleus; cytosol GO_0005739; GO_0005634; GO_0005829 1160 YDR097C MSH6 Protein required for mismatch repair in mitosis and meiosis Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p C HDA; HDA; IPI nucleus; cytoplasm; MutSalpha complex GO_0005634; GO_0005737; GO_0032301 1161 YDR098C GRX3 Glutathione-dependent oxidoreductase Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication C IPI; IDA nucleus; cytosol GO_0005634; GO_0005829 1162 YDR098C-A YDR098C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1163 YDR098C-B YDR098C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1164 YDR099W BMH2 14-3-3 protein, minor isoform 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; BMH2 has a paralog, BMH1, that arose from the whole genome duplication C IDA; IDA; HDA nucleus; cytoplasm; plasma membrane GO_0005634; GO_0005737; GO_0005886 1165 YDR100W TVP15 Integral membrane protein Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p C HDA; IDA COPI-coated vesicle; integral component of Golgi membrane GO_0030137; GO_0030173 1166 YDR101C ARX1 Nuclear export factor for the ribosomal pre-60S subunit Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex C IDA; IDA; IDA; IDA; IDA; IDA cytoplasm; nucleus; nucleoplasm; preribosome, large subunit precursor; cytosolic large ribosomal subunit; nucleolus GO_0005737; GO_0005634; GO_0005654; GO_0030687; GO_0022625; GO_0005730 1167 YDR102C YDR102C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; homozygous diploid deletion strain exhibits high budding index 1168 YDR103W STE5 Pheromone-responsive MAPK scaffold protein Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation C IDA; IDA; IDA; IDA cytoplasm; mating projection tip; plasma membrane; nucleus GO_0005737; GO_0043332; GO_0005886; GO_0005634 1169 YDR104C SPO71 Meiosis-specific protein required for prospore membrane morphogenesis Meiosis-specific protein required for spore wall formation; localizes to prospore membrane (PSM) and is required for PSM closure during sporulation; mediates PSM size; interacts with Spo1p and Vps13p and recruits Vps13p to the PSM during sporulation; mutants exhibit reduction in PSM PtdIns-phosphate pools; dispensable for both nuclear divisions during meiosis; contains two PH domains C IDA; IDA plasma membrane; prospore membrane GO_0005886; GO_0005628 1170 YDR105C TMS1 Vacuolar membrane protein of unknown function Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance C HDA fungal-type vacuole membrane GO_0000329 1171 YDR106W ARP10 Component of the dynactin complex Component of the dynactin complex; localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex C IDA; IPI cytoplasm; dynactin complex GO_0005737; GO_0005869 1172 YDR107C TMN2 Protein with a role in cellular adhesion and filamentous growth Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication C IDA fungal-type vacuole membrane GO_0000329 1173 YDR108W TRS85 Component of transport protein particle (TRAPP) complex III Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role C IDA; IDA; IDA; HDA; IDA pre-autophagosomal structure; TRAPPIII protein complex; cytoplasmic vesicle; cytosol; Golgi apparatus GO_0000407; GO_1990072; GO_0031410; GO_0005829; GO_0005794 1174 YDR109C YDR109C D-ribulokinase Putative kinase 1175 YDR110W FOB1 Nucleolar protein that binds the rDNA replication fork barrier site Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases C IDA; IDA rDNA heterochromatin; nucleolus GO_0033553; GO_0005730 1176 YDR111C ALT2 Catalytically inactive alanine transaminase Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1177 YDR112W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ALT2/YDR111C; null mutant displays increased levels of spontaneous Rad52p foci 1178 YDR113C PDS1 Securin Securin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation C IDA; IDA; IDA cytoplasm; nucleus; spindle GO_0005737; GO_0005634; GO_0005819 1179 YDR114C YDR114C Putative protein of unknown function Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium 1180 YDR115W MRX14 Putative mitochondrial ribosomal protein of the large subunit Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 1181 YDR116C MRPL1 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1182 YDR117C TMA64 Protein of unknown function that associates with ribosomes Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity C IDA ribosome GO_0005840 1183 YDR118W APC4 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress C HDA; IDA; IDA nucleus; nuclear periphery; anaphase-promoting complex GO_0005634; GO_0034399; GO_0005680 1184 YDR118W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified using a combination of expression profiling and mass spectrometry 1185 YDR119W VBA4 Protein of unknown function Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene C HDA fungal-type vacuole membrane GO_0000329 1186 YDR119W-A COX26 Stabilizes or regulates formation of respiratory chain supercomplexes Putative protein of unknown function; copurifies with respiratory chain supercomplexes composed of Complex III (ubiquinol-cytochrome c reductase) and Complex IV (cytochrome c oxidase) C IDA; IMP; HDA; IDA mitochondrial respiratory chain supercomplex; mitochondrial respiratory chain complex IV; mitochondrion; mitochondrial inner membrane GO_0097249; GO_0005751; GO_0005739; GO_0005743 1187 YDR120C TRM1 tRNA methyltransferase tRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB C IDA; IDA; IDA nuclear inner membrane; mitochondrion; nuclear envelope GO_0005637; GO_0005739; GO_0005635 1188 YDR121W DPB4 Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization C IDA; IPI CHRAC; epsilon DNA polymerase complex GO_0008623; GO_0008622 1189 YDR122W KIN1 Serine/threonine protein kinase involved in regulation of exocytosis Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication C IPI; IDA plasma membrane; cytoplasmic side of plasma membrane GO_0005886; GO_0009898 1190 YDR123C INO2 Transcription factor Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift C IPI; IDA RNA polymerase II transcription factor complex; nucleus GO_0090575; GO_0005634 1191 YDR124W YDR124W Putative protein of unknown function Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor 1192 YDR125C ECM18 Protein of unknown function Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 1193 YDR126W SWF1 Palmitoyltransferase that acts on transmembrane proteins Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion C IDA; IDA; IDA; HDA actin filament bundle; nuclear outer membrane-endoplasmic reticulum membrane network; actin cortical patch; endoplasmic reticulum GO_0032432; GO_0042175; GO_0030479; GO_0005783 1194 YDR127W ARO1 Pentafunctional arom protein Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids C HDA cytoplasm GO_0005737 1195 YDR128W MTC5 Subunit of SEACAT, a subcomplex of the SEA complex Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress C HDA; IDA; IDA fungal-type vacuole membrane; extrinsic component of fungal-type vacuolar membrane; Seh1-associated complex GO_0000329; GO_0097042; GO_0035859 1196 YDR129C SAC6 Fimbrin, actin-bundling protein Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress C IDA; HDA; HDA; IDA; HDA; HDA actin cortical patch; cellular bud tip; plasma membrane; actin filament bundle; cytoplasm; mating projection tip GO_0030479; GO_0005934; GO_0005886; GO_0032432; GO_0005737; GO_0043332 1197 YDR130C FIN1 Spindle pole body-related intermediate filament protein Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; HDA; IDA; IPI; HDA; IDA; IDA; IDA intermediate filament; mitotic spindle; spindle pole body; spindle microtubule; protein phosphatase type 1 complex; cytoplasm; nucleus; astral microtubule; condensed nuclear chromosome kinetochore GO_0005882; GO_0072686; GO_0005816; GO_0005876; GO_0000164; GO_0005737; GO_0005634; GO_0000235; GO_0000778 1198 YDR131C YDR131C F-box protein subunit of SCF ubiquitin ligase complex F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex C IPI SCF ubiquitin ligase complex GO_0019005 1199 YDR132C YDR132C Protein of unknown function Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication C HDA; HDA; HDA cytoplasm; nucleus; mitochondrion GO_0005737; GO_0005634; GO_0005739 1200 YDR133C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YDR134C 1201 YDR135C YCF1 Vacuolar glutathione S-conjugate transporter Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR C IDA fungal-type vacuole membrane GO_0000329 1202 YDR136C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect 1203 YDR137W RGP1 Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p C IPI; IDA; IDA; IPI RIC1-RGP1 guanyl-nucleotide exchange factor complex; guanyl-nucleotide exchange factor complex; Golgi apparatus; Golgi membrane GO_0034066; GO_0032045; GO_0005794; GO_0000139 1204 YDR138W HPR1 Subunit of THO/TREX complexes Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p C IPI; IMP; IDA; IMP Cdc73/Paf1 complex; nucleoplasmic THO complex; chromosome, telomeric region; THO complex part of transcription export complex GO_0016593; GO_0000446; GO_0000781; GO_0000445 1205 YDR139C RUB1 Ubiquitin-like protein with similarity to mammalian NEDD8 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) C HDA cytoplasm GO_0005737 1206 YDR140W MTQ2 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family C HDA; HDA; IPI nucleus; cytoplasm; eRF1 methyltransferase complex GO_0005634; GO_0005737; GO_0035657 1207 YDR141C DOP1 Golgi-localized, leucine-zipper domain containing protein Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies C HDA; IDA; HDA; IDA; HDA mitochondrion; endosome; cytoplasm; trans-Golgi network; cytosol GO_0005739; GO_0005768; GO_0005737; GO_0005802; GO_0005829 1208 YDR142C PEX7 Peroxisomal signal receptor for peroxisomal matrix proteins Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) C IDA; IDA peroxisome; cytosol GO_0005777; GO_0005829 1209 YDR143C SAN1 Ubiquitin-protein ligase Ubiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins C IMP; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1210 YDR144C MKC7 GPI-anchored aspartyl protease GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication C IDA; HDA fungal-type cell wall; fungal-type vacuole GO_0009277; GO_0000324 1211 YDR145W TAF12 Subunit (61/68 kDa) of TFIID and SAGA complexes Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A C IDA; IDA; IDA SAGA complex; SLIK (SAGA-like) complex; transcription factor TFIID complex GO_0000124; GO_0046695; GO_0005669 1212 YDR146C SWI5 Transcription factor that recruits Mediator and Swi/Snf complexes Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1213 YDR147W EKI1 Ethanolamine kinase Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1214 YDR148C KGD2 Dihydrolipoyl transsuccinylase Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated C IDA; HDA; IDA mitochondrial nucleoid; mitochondrion; mitochondrial oxoglutarate dehydrogenase complex GO_0042645; GO_0005739; GO_0009353 1215 YDR149C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1/YDR150W; null mutation blocks anaerobic growth 1216 YDR150W NUM1 Protein required for nuclear migration Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and motochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex C HDA; IDA; IDA; IDA mitochondrion; cell cortex; endoplasmic reticulum; cellular bud tip GO_0005739; GO_0005938; GO_0005783; GO_0005934 1217 YDR151C CTH1 Member of the CCCH zinc finger family Member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication 1218 YDR152W GIR2 Highly-acidic RWD domain-containing cytoplasmic protein Highly-acidic RWD domain-containing protein of unknown function; cytoplasmic; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein C IDA; HDA polysome; cytoplasm GO_0005844; GO_0005737 1219 YDR153C ENT5 Protein containing an N-terminal epsin-like domain Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin C IDA; IDA; HDA endosome; clathrin vesicle coat; cytoplasm GO_0005768; GO_0030125; GO_0005737 1220 YDR154C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein 1221 YDR155C CPR1 Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin) Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress C IPI; IDA; IDA; HDA; IDA histone deacetylase complex; mitochondrial intermembrane space; Set3 complex; mitochondrion; nucleus GO_0000118; GO_0005758; GO_0034967; GO_0005739; GO_0005634 1222 YDR156W RPA14 RNA polymerase I subunit A14 RNA polymerase I subunit A14 C IDA DNA-directed RNA polymerase I complex GO_0005736 1223 YDR157W YDR157W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1224 YDR158W HOM2 Aspartic beta semi-aldehyde dehydrogenase Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis C HDA; HDA; HDA cytoplasm; plasma membrane; nucleus GO_0005737; GO_0005886; GO_0005634 1225 YDR159W SAC3 mRNA export factor mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) C IDA; IDA nuclear pore; transcription export complex 2 GO_0005643; GO_0070390 1226 YDR160W SSY1 Component of the SPS plasma membrane amino acid sensor system Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p C IDA; HDA plasma membrane; endoplasmic reticulum GO_0005886; GO_0005783 1227 YDR161W ACL4 Specific assembly chaperone for ribosomal protein Rpl4p Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1228 YDR162C NBP2 Protein involved in the HOG (high osmolarity glycerol) pathway Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) C HDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1229 YDR163W CWC15 Non-essential protein involved in pre-mRNA splicing Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p C IDA U2-type spliceosomal complex GO_0005684 1230 YDR164C SEC1 Sm-like protein involved in docking and fusion of exocytic vesicles Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p C IDA; HDA; IDA; IDA cellular bud neck; cell periphery; cellular bud tip; plasma membrane GO_0005935; GO_0071944; GO_0005934; GO_0005886 1231 YDR165W TRM82 Noncatalytic subunit of a tRNA methyltransferase complex Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia C IDA; HDA; IDA tRNA methyltransferase complex; nucleus; cytosol GO_0043527; GO_0005634; GO_0005829 1232 YDR166C SEC5 Essential 107kDa subunit of the exocyst complex Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip C IDA; HDA; HDA; IDA; IDA; HDA; HDA exocyst; cell periphery; prospore membrane; incipient cellular bud site; cellular bud tip; mating projection tip; cellular bud neck GO_0000145; GO_0071944; GO_0005628; GO_0000131; GO_0005934; GO_0043332; GO_0005935 1233 YDR167W TAF10 Subunit (145 kDa) of TFIID and SAGA complexes Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification C IDA; IDA; IDA SAGA complex; transcription factor TFIID complex; SLIK (SAGA-like) complex GO_0000124; GO_0005669; GO_0046695 1234 YDR168W CDC37 Essential Hsp90p co-chaperone Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding C HDA cytoplasm GO_0005737 1235 YDR169C STB3 Ribosomal RNA processing element (RRPE)-binding protein Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1236 YDR169C-A YDR169C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 1237 YDR170C SEC7 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles C IDA; IDA; IDA; IDA trans-Golgi network; cytosol; late endosome; Golgi-associated vesicle GO_0005802; GO_0005829; GO_0005770; GO_0005798 1238 YDR170W-A YDR170W-A Retrotransposon TYA Gag gene Retrotransposon TYA Gag gene; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag; YDR170W-A is part of a mutant retrotransposon; distribution in the cytoplasm becomes irregular rather than punctate upon DNA replication stress C HDA cytoplasm GO_0005737 1239 YDR171W HSP42 Small heat shock protein (sHSP) with chaperone activity Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress C IPI; IPI; HDA chaperonin-containing T-complex; cytoskeleton; cytoplasm GO_0005832; GO_0005856; GO_0005737 1240 YDR172W SUP35 Translation termination factor eRF3 Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)] C IDA; IDA; HDA cytoplasmic stress granule; translation release factor complex; cytosol GO_0010494; GO_0018444; GO_0005829 1241 YDR173C ARG82 Inositol polyphosphate multikinase (IPMK) Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes C IDA nucleus GO_0005634 1242 YDR174W HMO1 Chromatin associated high mobility group (HMG) family member Chromatin associated high mobility group (HMG) family member; involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA; IDA; IDA cytoplasm; cytosol; nuclear chromatin; rDNA heterochromatin; nucleolus; small-subunit processome; nucleus GO_0005737; GO_0005829; GO_0000790; GO_0033553; GO_0005730; GO_0032040; GO_0005634 1243 YDR175C RSM24 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1244 YDR176W NGG1 Subunit of chromatin modifying histone acetyltransferase complexes Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes C IDA; IDA; IDA SLIK (SAGA-like) complex; Ada2/Gcn5/Ada3 transcription activator complex; SAGA complex GO_0046695; GO_0005671; GO_0000124 1245 YDR177W UBC1 Ubiquitin-conjugating enzyme Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC) C HDA; IPI cytosol; proteasome complex GO_0005829; GO_0000502 1246 YDR178W SDH4 Membrane anchor subunit of succinate dehydrogenase (SDH) Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma C HDA mitochondrion GO_0005739 1247 YDR179C CSN9 Subunit of the Cop9 signalosome Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling C IDA COP9 signalosome GO_0008180 1248 YDR179W-A NVJ3 Protein with a potential role in tethering ER and vacuoles Putative protein of unknown function C IDA nucleus-vacuole junction GO_0071561 1249 YDR180W SCC2 Subunit of cohesin loading factor (Scc2p-Scc4p) Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA; IDA; IDA SMC loading complex; nuclear chromatin; nucleus; 2-micrometer circle DNA; cytosol GO_0032116; GO_0000790; GO_0005634; GO_0005729; GO_0005829 1250 YDR181C SAS4 Subunit of the SAS complex (Sas2p, Sas4p, Sas5p) Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p C IDA; IDA nuclear chromatin; SAS acetyltransferase complex GO_0000790; GO_0033255 1251 YDR182W CDC1 Putative mannose-ethanolamine phosphate phosphodiesterase Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution C IDA endoplasmic reticulum GO_0005783 1252 YDR182W-A YDR182W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 1253 YDR183C-A Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR 1254 YDR183W PLP1 Protein that interacts with CCT (chaperonin containing TCP-1) complex Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators 1255 YDR184C ATC1 Nuclear protein Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1256 YDR185C UPS3 Mitochondrial protein of unknown function Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication C HDA; IDA; IDA mitochondrion; mitochondrial intermembrane space; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0005758; GO_0031314 1257 YDR186C SND1 Protein involved in SRP-independent targeting of substrates to the ER Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA; HDA ribosome; cytoplasm GO_0005840; GO_0005737 1258 YDR187C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W 1259 YDR188W CCT6 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif C IDA chaperonin-containing T-complex GO_0005832 1260 YDR189W SLY1 Hydrophilic protein involved in ER/Golgi vesicle trafficking Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex C IDA; IDA; IDA; IDA; HDA endoplasmic reticulum; Golgi membrane; SNARE complex; ER to Golgi transport vesicle; cytosol GO_0005783; GO_0000139; GO_0031201; GO_0030134; GO_0005829 1261 YDR190C RVB1 ATP-dependent DNA helicase, also known as pontin ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly C IPI; IDA; IPI; HDA; IPI Swr1 complex; nucleus; R2TP complex; ASTRA complex; Ino80 complex GO_0000812; GO_0005634; GO_0097255; GO_0070209; GO_0031011 1262 YDR191W HST4 NAD(+)-dependent protein deacetylase Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism C HDA; IDA; IDA nucleus; mitochondrion; cytoplasm GO_0005634; GO_0005739; GO_0005737 1263 YDR192C NUP42 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p C IDA; IDA; IDA nuclear pore; nuclear pore central transport channel; nuclear pore cytoplasmic filaments GO_0005643; GO_0044613; GO_0044614 1264 YDR193W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1265 YDR194C MSS116 Mitochondrial transcription elongation factor Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate C HDA; IMP mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 1266 YDR194W-A YDR194W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 1267 YDR195W REF2 RNA-binding protein RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; relocalizes to the cytosol in response to hypoxia C HDA; IDA; IDA nucleus; mRNA cleavage and polyadenylation specificity factor complex; cytosol GO_0005634; GO_0005847; GO_0005829 1268 YDR196C CAB5 Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets C IDA; HDA; HDA; HDA; IDA; HDA lipid particle; mitochondrion; cytosol; nuclear envelope; CoA-synthesizing protein complex; endoplasmic reticulum GO_0005811; GO_0005739; GO_0005829; GO_0005635; GO_1990143; GO_0005783 1269 YDR197W CBS2 Mitochondrial translational activator of the COB mRNA Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader C IPI; IDA; HDA mitochondrial ribosome; extrinsic component of mitochondrial inner membrane; mitochondrion GO_0005761; GO_0031314; GO_0005739 1270 YDR198C RKM2 Ribosomal protein lysine methyltransferase Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp 1271 YDR199W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64/YDR200C; computationally predicted to have thiol-disulfide oxidoreductase activity 1272 YDR200C VPS64 Protein required for cytoplasm to vacuole targeting of proteins Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication C HDA; IDA; IDA; IDA cytoplasm; endoplasmic reticulum; endoplasmic reticulum-Golgi intermediate compartment; endoplasmic reticulum membrane GO_0005737; GO_0005783; GO_0005793; GO_0005789 1273 YDR201W SPC19 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body C IDA DASH complex GO_0042729 1274 YDR202C RAV2 Subunit of RAVE complex (Rav1p, Rav2p, Skp1p) Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme C IPI RAVE complex GO_0043291 1275 YDR203W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAV2/YDR202C 1276 YDR204W COQ4 Protein with a role in ubiquinone (Coenzyme Q) biosynthesis Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 1277 YDR205W MSC2 Endoplasmic reticulum zinc transporter Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids C IDA endoplasmic reticulum GO_0005783 1278 YDR206W EBS1 Protein involved in translation inhibition and nonsense-mediated decay Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication C IDA; IDA P-body; cytoplasm GO_0000932; GO_0005737 1279 YDR207C UME6 Rpd3L histone deacetylase complex subunit Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p C HDA; HDA Rpd3L complex; Rpd3L-Expanded complex GO_0033698; GO_0070210 1280 YDR208W MSS4 Phosphatidylinositol-4-phosphate 5-kinase Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation C IDA; IDA plasma membrane; nucleus GO_0005886; GO_0005634 1281 YDR209C YDR209C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W 1282 YDR210C-C YDR210C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1283 YDR210C-D YDR210C-D Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1284 YDR210W YDR210W Predicted tail-anchored plasma membrane protein Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 1285 YDR210W-A YDR210W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1286 YDR210W-B YDR210W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1287 YDR211W GCD6 Catalytic epsilon subunit of the translation initiation factor eIF2B Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress C IPI; IDA; HDA; HDA eukaryotic translation initiation factor 2B complex; guanyl-nucleotide exchange factor complex; cytosol; cytoplasm GO_0005851; GO_0032045; GO_0005829; GO_0005737 1288 YDR212W TCP1 Alpha subunit of chaperonin-containing T-complex Alpha subunit of chaperonin-containing T-complex; complex mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein C HDA; IDA plasma membrane; chaperonin-containing T-complex GO_0005886; GO_0005832 1289 YDR213W UPC2 Sterol regulatory element binding protein Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication C IDA; HDA; IDA perinuclear region of cytoplasm; cytoplasm; membrane GO_0048471; GO_0005737; GO_0016020 1290 YDR214W AHA1 Co-chaperone that binds Hsp82p and activates its ATPase activity Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress C IPI cytoplasm GO_0005737 1291 YDR215C YDR215C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein 1292 YDR216W ADR1 Carbon source-responsive zinc-finger transcription factor Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization C HDA; HDA; IDA cytosol; cytoplasm; nucleus GO_0005829; GO_0005737; GO_0005634 1293 YDR217C RAD9 DNA damage-dependent checkpoint protein DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p C IDA chromatin GO_0000785 1294 YDR218C SPR28 Sporulation-specific homolog of the CDC3/10/11/12 family of genes Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes C IDA; HDA; IDA; IDA; HDA; IDA meiotic spindle; septin complex; prospore membrane; ascospore wall; cytosol; spindle microtubule GO_0072687; GO_0031105; GO_0005628; GO_0005619; GO_0005829; GO_0005876 1295 YDR219C MFB1 Mitochondria-associated F-box protein Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding C IDA extrinsic component of mitochondrial outer membrane GO_0031315 1296 YDR220C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein 1297 YDR221W GTB1 Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress C HDA; IPI; IDA endoplasmic reticulum; glucosidase II complex; endoplasmic reticulum lumen GO_0005783; GO_0017177; GO_0005788 1298 YDR222W YDR222W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1299 YDR223W CRF1 Transcriptional corepressor Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; cytoplasm; nuclear chromatin GO_0005634; GO_0005737; GO_0000790 1300 YDR224C HTB1 Histone H2B Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation C IDA; IDA replication fork protection complex; nuclear nucleosome GO_0031298; GO_0000788 1301 YDR225W HTA1 Histone H2A Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo C IDA replication fork protection complex GO_0031298 1302 YDR226W ADK1 Adenylate kinase, required for purine metabolism Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA cytoplasm; mitochondrion; mitochondrial intermembrane space GO_0005737; GO_0005739; GO_0005758 1303 YDR227W SIR4 SIR protein involved in assembly of silent chromatin domains SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan C IMP; IDA; IDA nuclear chromosome, telomeric region; nuclear telomeric heterochromatin; chromatin silencing complex GO_0000784; GO_0005724; GO_0005677 1304 YDR228C PCF11 mRNA 3' end processing factor mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA mRNA cleavage factor complex; cytosol; nucleus GO_0005849; GO_0005829; GO_0005634 1305 YDR229W IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase C IDA fungal-type vacuole membrane GO_0000329 1306 YDR230W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20/YDR231C 1307 YDR231C COX20 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 1308 YDR232W HEM1 5-aminolevulinate synthase 5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 1309 YDR233C RTN1 Reticulon protein Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; HDA; IDA; IDA mitochondrion; Golgi apparatus; integral component of endoplasmic reticulum membrane; cell periphery; endoplasmic reticulum; cortical endoplasmic reticulum; endoplasmic reticulum tubular network GO_0005739; GO_0005794; GO_0030176; GO_0071944; GO_0005783; GO_0032541; GO_0071782 1310 YDR234W LYS4 Homoaconitase Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway C HDA mitochondrion GO_0005739 1311 YDR235W PRP42 U1 snRNP protein involved in splicing U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats C IDA; IDA U1 snRNP; U2-type prespliceosome GO_0005685; GO_0071004 1312 YDR236C FMN1 Riboflavin kinase, produces riboflavin monophosphate (FMN) Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane C IDA; IDA mitochondrial inner membrane; intracellular membrane-bounded organelle GO_0005743; GO_0043231 1313 YDR237W MRPL7 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1314 YDR238C SEC26 Essential beta-coat protein of the COPI coatomer Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) C IGI COPI vesicle coat GO_0030126 1315 YDR239C YDR239C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments C HDA; HDA ribosome; cytoplasm GO_0005840; GO_0005737 1316 YDR240C SNU56 Component of U1 snRNP required for mRNA splicing via spliceosome Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex C IDA; IDA; IPI U1 snRNP; U2-type prespliceosome; commitment complex GO_0005685; GO_0071004; GO_0000243 1317 YDR241W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay 1318 YDR242W AMD2 Putative amidase Putative amidase 1319 YDR243C PRP28 RNA binding protein RNA helicase in the DEAD-box family; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site C IDA U5 snRNP GO_0005682 1320 YDR244W PEX5 Peroxisomal membrane signal receptor for peroxisomal matrix proteins Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions C IDA; IDA; IDA; IDA peroxisomal membrane; cytosol; peroxisomal importomer complex; peroxisome GO_0005778; GO_0005829; GO_1990429; GO_0005777 1321 YDR245W MNN10 Subunit of a Golgi mannosyltransferase complex Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p C HDA; IDA endoplasmic reticulum; alpha-1,6-mannosyltransferase complex GO_0005783; GO_0000136 1322 YDR246W TRS23 Core component of transport protein particle (TRAPP) complexes I-III Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4 C IDA; IDA; IDA TRAPPIII protein complex; TRAPPII protein complex; TRAPPI protein complex GO_1990072; GO_1990071; GO_1990070 1323 YDR246W-A YDR246W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 1324 YDR247W VHS1 Cytoplasmic serine/threonine protein kinase Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1325 YDR248C YDR248C Putative gluconokinase Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 C HDA; HDA fungal-type vacuole; cytoplasm GO_0000324; GO_0005737 1326 YDR249C YDR249C Putative protein of unknown function Putative protein of unknown function 1327 YDR250C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1328 YDR251W PAM1 Essential protein of unknown function Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication C HDA; HDA cellular bud; cellular bud neck GO_0005933; GO_0005935 1329 YDR252W BTT1 Heterotrimeric nascent polypeptide-associated complex beta3 subunit Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication C IPI; IDA CCR4-NOT core complex; nascent polypeptide-associated complex GO_0030015; GO_0005854 1330 YDR253C MET32 Zinc-finger DNA-binding transcription factor Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1331 YDR254W CHL4 Outer kinetochore protein required for chromosome stability Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 C IDA condensed nuclear chromosome outer kinetochore GO_0000942 1332 YDR255C RMD5 Component of GID Complex that confers ubiquitin ligase (U3) activity Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain C IDA; IDA; IDA; HDA GID complex; cytosol; peroxisome; nucleus GO_0034657; GO_0005829; GO_0005777; GO_0005634 1333 YDR256C CTA1 Catalase A Catalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation C IDA; IDA peroxisomal matrix; mitochondrial matrix GO_0005782; GO_0005759 1334 YDR257C RKM4 Ribosomal lysine methyltransferase Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein C HDA nucleus GO_0005634 1335 YDR258C HSP78 Oligomeric mitochondrial matrix chaperone Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 1336 YDR259C YAP6 Basic leucine zipper (bZIP) transcription factor Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication C IDA nucleus GO_0005634 1337 YDR260C SWM1 Subunit of the anaphase-promoting complex (APC) Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation C IDA; IDA anaphase-promoting complex; nucleus GO_0005680; GO_0005634 1338 YDR261C EXG2 Exo-1,3-beta-glucanase Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor C IDA fungal-type cell wall GO_0009277 1339 YDR261C-C YDR261C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably non-functional 1340 YDR261C-D YDR261C-D Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional C IDA nucleus GO_0005634 1341 YDR261W-A YDR261W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1342 YDR261W-B YDR261W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1343 YDR262W YDR262W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment C HDA fungal-type vacuole GO_0000324 1344 YDR263C DIN7 Mitochondrial nuclease functioning in DNA repair and replication Mitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 1345 YDR264C AKR1 Palmitoyl transferase involved in protein palmitoylation Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication C IDA; IDA membrane; Golgi apparatus GO_0016020; GO_0005794 1346 YDR265W PEX10 Peroxisomal membrane E3 ubiquitin ligase Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders C IDA; IDA; IDA peroxisomal importomer complex; peroxisomal membrane; integral component of peroxisomal membrane GO_1990429; GO_0005778; GO_0005779 1347 YDR266C HEL2 RING finger ubiquitin ligase (E3) RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor C HDA; HDA ribosome; cytoplasm GO_0005840; GO_0005737 1348 YDR267C CIA1 Component of cytosolic iron-sulfur protein assembly (CIA) machinery Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein C IDA; IDA; HDA cytosol; nucleus; cytoplasm GO_0005829; GO_0005634; GO_0005737 1349 YDR268W MSW1 Mitochondrial tryptophanyl-tRNA synthetase Mitochondrial tryptophanyl-tRNA synthetase C HDA mitochondrion GO_0005739 1350 YDR269C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1351 YDR270W CCC2 Cu(+2)-transporting P-type ATPase Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism C IDA trans-Golgi network transport vesicle membrane GO_0012510 1352 YDR271C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W 1353 YDR272W GLO2 Cytoplasmic glyoxalase II Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 1354 YDR273W DON1 Meiosis-specific component of the spindle pole body Meiosis-specific component of the spindle pole body; part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; DON1 has a paralog, CUE5, that arose from the whole genome C IDA; IDA prospore membrane; prospore membrane leading edge GO_0005628; GO_0070056 1355 YDR274C YDR274C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1356 YDR275W BSC2 Protein of unknown function Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication C HDA lipid particle GO_0005811 1357 YDR276C PMP3 Small plasma membrane protein Small plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 1358 YDR277C MTH1 Negative regulator of the glucose-sensing signal transduction pathway Negative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication 1359 YDR278C YDR278C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1360 YDR279W RNH202 Ribonuclease H2 subunit Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome C IDA; HDA ribonuclease H2 complex; nucleus GO_0032299; GO_0005634 1361 YDR280W RRP45 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress C IDA; IDA; IDA nuclear exosome (RNase complex); cytoplasmic exosome (RNase complex); nucleolus GO_0000176; GO_0000177; GO_0005730 1362 YDR281C PHM6 Protein of unknown function Protein of unknown function; expression is regulated by phosphate levels C HDA fungal-type vacuole GO_0000324 1363 YDR282C MRX10 Mitochondrial inner membrane protein of unknown function Mitochondrial inner membrane protein of unknown function; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation C IDA mitochondrial inner membrane GO_0005743 1364 YDR283C GCN2 Protein kinase Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control C IDA cytosolic ribosome GO_0022626 1365 YDR284C DPP1 Diacylglycerol pyrophosphate (DGPP) phosphatase Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism C IDA fungal-type vacuole membrane GO_0000329 1366 YDR285W ZIP1 Transverse filament protein of the synaptonemal complex Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-allelc sites; potential Cdc28p substrate C IDA; IDA; IDA chromosome, centromeric region; synaptonemal complex; transverse filament GO_0000775; GO_0000795; GO_0000802 1367 YDR286C MGP12 Putative protein of unknown function Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site 1368 YDR287W INM2 Inositol monophosphatase Inositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy 1369 YDR288W NSE3 Component of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress C IDA; HDA; HDA Smc5-Smc6 complex; nucleus; cytoplasm GO_0030915; GO_0005634; GO_0005737 1370 YDR289C RTT103 Protein involved in transcription termination by RNA polymerase II Protein involved in transcription termination by RNA polymerase II; interacts with exonuclease Rat1p and Rai1p; has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition C IDA; IDA site of double-strand break; nuclear chromatin GO_0035861; GO_0000790 1371 YDR290W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 1372 YDR291W HRQ1 3'-5' DNA helicase that has DNA strand annealing activity 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS) C HDA nucleus GO_0005634 1373 YDR292C SRP101 Signal recognition particle (SRP) receptor alpha subunit Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p C IPI; IDA; HDA signal recognition particle receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum GO_0005785; GO_0005789; GO_0005783 1374 YDR293C SSD1 Translational repressor with a role in polar growth and wall integrity Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function C IDA; IDA; IDA; IDA; IDA; IDA cytoplasm; cytoplasmic stress granule; cellular bud neck; nucleus; cellular bud; P-body GO_0005737; GO_0010494; GO_0005935; GO_0005634; GO_0005933; GO_0000932 1375 YDR294C DPL1 Dihydrosphingosine phosphate lyase Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate C HDA; IDA; IDA endoplasmic reticulum; cortical endoplasmic reticulum; perinuclear endoplasmic reticulum GO_0005783; GO_0032541; GO_0097038 1376 YDR295C HDA2 Subunit of the HDA1 histone deacetylase complex Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia C IDA; IPI; IDA nucleus; HDA1 complex; cytosol GO_0005634; GO_0070823; GO_0005829 1377 YDR296W MHR1 Mitochondrial ribosomal protein of the large subunit Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress C HDA; IDA; IDA mitochondrion; mitochondrial large ribosomal subunit; nucleus GO_0005739; GO_0005762; GO_0005634 1378 YDR297W SUR2 Sphinganine C4-hydroxylase Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis C IDA endoplasmic reticulum membrane GO_0005789 1379 YDR298C ATP5 Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated C HDA; IMP mitochondrion; mitochondrial proton-transporting ATP synthase, stator stalk GO_0005739; GO_0000274 1380 YDR299W BFR2 Component of the SSU and 90S preribosomes Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock C IDA; HDA; IMP nucleolus; 90S preribosome; small-subunit processome GO_0005730; GO_0030686; GO_0032040 1381 YDR300C PRO1 Gamma-glutamyl kinase Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1382 YDR301W CFT1 RNA-binding subunit of the mRNA cleavage and polyadenylation factor RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF C HDA; IDA; HDA; IPI mitochondrion; mRNA cleavage and polyadenylation specificity factor complex; nucleus; mRNA cleavage factor complex GO_0005739; GO_0005847; GO_0005634; GO_0005849 1383 YDR302W GPI11 ER membrane protein involved in a late step of GPI anchor assembly ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog C HDA endoplasmic reticulum GO_0005783 1384 YDR303C RSC3 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication C HDA; IDA nucleus; RSC complex GO_0005634; GO_0016586 1385 YDR304C CPR5 Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 1386 YDR305C HNT2 Dinucleoside triphosphate hydrolase Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 1387 YDR306C YDR306C F-box protein of unknown function F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain C IDA SCF ubiquitin ligase complex GO_0019005 1388 YDR307W PMT7 Putative protein mannosyltransferase similar to Pmt1p Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation C HDA endoplasmic reticulum GO_0005783 1389 YDR308C SRB7 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p C IDA; IDA mediator complex; core mediator complex GO_0016592; GO_0070847 1390 YDR309C GIC2 Redundant rho-like GTPase Cdc42p effector Redundant rho-like GTPase Cdc42p effector; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; GIC2 has a paralog, GIC1, that arose from the whole genome duplication C IGI; IDA; IDA; IDA plasma membrane; mating projection tip; incipient cellular bud site; cellular bud tip GO_0005886; GO_0043332; GO_0000131; GO_0005934 1391 YDR310C SUM1 Transcriptional repressor that regulates middle-sporulation genes Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint C IDA nucleus GO_0005634 1392 YDR311W TFB1 Subunit of TFIIH and nucleotide excision repair factor 3 complexes Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA nucleotide-excision repair factor 3 complex; nucleus; core TFIIH complex; cytosol; holo TFIIH complex GO_0000112; GO_0005634; GO_0000439; GO_0005829; GO_0005675 1393 YDR312W SSF2 Protein required for ribosomal large subunit maturation Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication C IDA nucleolus GO_0005730 1394 YDR313C PIB1 RING-type ubiquitin ligase of the endosomal and vacuolar membranes RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain C IDA; IDA late endosome; fungal-type vacuole membrane GO_0005770; GO_0000329 1395 YDR314C RAD34 Protein involved in nucleotide excision repair (NER) Protein involved in nucleotide excision repair (NER); homologous to RAD4 C HDA nucleus GO_0005634 1396 YDR315C IPK1 Inositol 1,3,4,5,6-pentakisphosphate 2-kinase Inositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable C IDA nucleus GO_0005634 1397 YDR316W OMS1 Protein integral to the mitochondrial membrane Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations C IDA; HDA mitochondrial envelope; mitochondrion GO_0005740; GO_0005739 1398 YDR316W-A YDR316W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1399 YDR316W-B YDR316W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1400 YDR317W HIM1 Protein of unknown function involved in DNA repair Protein of unknown function involved in DNA repair 1401 YDR318W MCM21 Component of the kinetochore sub-complex COMA Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 C IDA; IDA COMA complex; condensed nuclear chromosome kinetochore GO_0000817; GO_0000778 1402 YDR319C YFT2 Protein required for normal ER membrane biosynthesis Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens 1403 YDR320C SWA2 Auxilin-like protein involved in vesicular transport Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles C HDA; IDA cytosol; endoplasmic reticulum membrane GO_0005829; GO_0005789 1404 YDR320C-A DAD4 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 1405 YDR320W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DAD4/YDR320C-A 1406 YDR321W ASP1 Cytosolic L-asparaginase, involved in asparagine catabolism Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively 1407 YDR322C-A TIM11 Subunit e of mitochondrial F1F0-ATPase Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae C IMP; HDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion GO_0000276; GO_0005739 1408 YDR322W MRPL35 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1409 YDR323C PEP7 Adaptor protein involved in vesicle-mediated vacuolar protein sorting Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance C IDA; HDA; HDA cytoplasmic side of endosome membrane; cytoplasm; nucleus GO_0010009; GO_0005737; GO_0005634 1410 YDR324C UTP4 Subunit of U3-containing 90S preribosome and SSU processome complexes Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript; Small Subunit processome is also known as SSU processome C IDA; IDA; IDA; IDA small-subunit processome; 90S preribosome; nucleolus; t-UTP complex GO_0032040; GO_0030686; GO_0005730; GO_0034455 1411 YDR325W YCG1 Subunit of the condensin complex Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation C IGI nuclear condensin complex GO_0000799 1412 YDR326C YSP2 Sterol-binding protein Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication C IDA; HDA; IDA; HDA cortical endoplasmic reticulum; cytoplasm; mitochondrion; cell periphery GO_0032541; GO_0005737; GO_0005739; GO_0071944 1413 YDR327W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SKP1 1414 YDR328C SKP1 Evolutionarily conserved kinetochore protein Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress C IGI; IPI; IPI; IDA; IDA CBF3 complex; SCF ubiquitin ligase complex; RAVE complex; nucleus; cytoplasm GO_0031518; GO_0019005; GO_0043291; GO_0005634; GO_0005737 1415 YDR329C PEX3 Peroxisomal membrane protein (PMP) Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p C IDA; IDA; IDA endoplasmic reticulum; peroxisomal membrane; peroxisome GO_0005783; GO_0005778; GO_0005777 1416 YDR330W UBX5 UBX domain-containing protein that interacts with Cdc48p UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1417 YDR331W GPI8 ER membrane glycoprotein subunit of the GPI transamidase complex ER membrane glycoprotein subunit of the GPI transamidase complex; adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog C HDA; IDA; IDA endoplasmic reticulum; GPI-anchor transamidase complex; endoplasmic reticulum membrane GO_0005783; GO_0042765; GO_0005789 1418 YDR332W IRC3 Double-stranded DNA-dependent helicase of the DExH/D-box family Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 1419 YDR333C RQC1 Component of the ribosome quality control complex (RQC) Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC C IDA; IDA; HDA cytosolic large ribosomal subunit; RQC complex; cytoplasm GO_0022625; GO_1990112; GO_0005737 1420 YDR334W SWR1 Swi2/Snf2-related ATPase Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction C IDA; IDA; IDA cytosol; Swr1 complex; nucleus GO_0005829; GO_0000812; GO_0005634 1421 YDR335W MSN5 Karyopherin Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1422 YDR336W MRX8 Protein that associates with mitochondrial ribosome Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 1423 YDR337W MRPS28 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1424 YDR338C YDR338C Putative protein of unknown function Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily 1425 YDR339C FCF1 Putative PINc domain nuclease Putative PINc domain nuclease; required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p C IDA; IDA small-subunit processome; nucleolus GO_0032040; GO_0005730 1426 YDR340W Putative protein of unknown function 1427 YDR341C YDR341C Arginyl-tRNA synthetase Arginyl-tRNA synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDR341C has a paralog, MSR1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 1428 YDR342C HXT7 High-affinity glucose transporter High-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels; HXT7 has a paralog, HXT4, that arose from the whole genome duplication C HDA; IDA; HDA cell periphery; plasma membrane; mitochondrion GO_0071944; GO_0005886; GO_0005739 1429 YDR343C HXT6 High-affinity glucose transporter High-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3; HXT6 has a paralog, HXT1, that arose from the whole genome duplication C HDA; HDA; HDA mitochondrion; cell periphery; plasma membrane GO_0005739; GO_0071944; GO_0005886 1430 YDR344C YDR344C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1431 YDR345C HXT3 Low affinity glucose transporter of the major facilitator superfamily Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 1432 YDR346C SVF1 Protein with a potential role in cell survival pathways Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1433 YDR347W MRP1 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1434 YDR348C PAL1 Protein of unknown function thought to be involved in endocytosis Protein of unknown function thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication C HDA; HDA; HDA; HDA; IDA cellular bud tip; cellular bud neck; cytoplasm; mating projection tip; cell cortex GO_0005934; GO_0005935; GO_0005737; GO_0043332; GO_0005938 1435 YDR349C YPS7 Putative GPI-anchored aspartic protease Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum C HDA; HDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 1436 YDR350C ATP22 Specific translational activator for the mitochondrial ATP6 mRNA Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane C IDA mitochondrial inner membrane GO_0005743 1437 YDR351W SBE2 Protein required for bud growth Protein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication C IDA Golgi apparatus GO_0005794 1438 YDR352W YPQ2 Putative vacuolar membrane transporter for cationic amino acids Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter C IDA; HDA integral component of fungal-type vacuolar membrane; fungal-type vacuole membrane GO_0071627; GO_0000329 1439 YDR353W TRR1 Cytoplasmic thioredoxin reductase Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication C IDA; HDA; IDA mitochondrial intermembrane space; mitochondrion; cytosol GO_0005758; GO_0005739; GO_0005829 1440 YDR354C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TRP4; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1441 YDR354W TRP4 Anthranilate phosphoribosyl transferase Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1442 YDR355C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W 1443 YDR356W SPC110 Inner plaque spindle pole body (SPB) component Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner C IDA; IDA; HDA central plaque of spindle pole body; inner plaque of spindle pole body; nucleus GO_0005823; GO_0005822; GO_0005634 1444 YDR357C CNL1 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA; IDA cytoplasm; BLOC-1 complex GO_0005737; GO_0031083 1445 YDR358W GGA1 Golgi-localized protein with homology to gamma-adaptin Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication C IDA; HDA trans-Golgi network; cytosol GO_0005802; GO_0005829 1446 YDR359C EAF1 Component of the NuA4 histone acetyltransferase complex Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 C IPI; IPI histone acetyltransferase complex; NuA4 histone acetyltransferase complex GO_0000123; GO_0035267 1447 YDR360W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C 1448 YDR361C BCP1 Essential protein involved in nuclear export of Mss4p Essential protein involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1449 YDR362C TFC6 Subunit of RNA polymerase III transcription initiation factor complex Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 C IDA transcription factor TFIIIC complex GO_0000127 1450 YDR363W ESC2 Sumo-like domain protein Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member C IDA nucleus GO_0005634 1451 YDR363W-A SEM1 19S proteasome regulatory particle lid subcomplex component Component of lid subcomplex of 26S proteasome regulatory subunit; involved in mRNA export mediated by TREX-2 complex (Sac3p-Thp1p); assumes different conformations in different contexts, functions as molecular glue stabilizing the Rpn3p/Rpn7p regulatory heterodimer, and tethers it to lid helical bundle; ortholog of human DSS1; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA proteasome regulatory particle, lid subcomplex; cytosol; transcription export complex 2; proteasome storage granule GO_0008541; GO_0005829; GO_0070390; GO_0034515 1452 YDR364C CDC40 Pre-mRNA splicing factor Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats C IDA; IDA nuclear periphery; spliceosomal complex GO_0034399; GO_0005681 1453 YDR365C ESF1 Nucleolar protein involved in pre-rRNA processing Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels C HDA; HDA nucleus; nucleolus GO_0005634; GO_0005730 1454 YDR365W-A YDR365W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1455 YDR365W-B YDR365W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1456 YDR366C YDR366C Putative protein of unknown function Putative protein of unknown function 1457 YDR367W KEI1 Component of inositol phosphorylceramide (IPC) synthase Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene C IDA; HDA; IPI; HDA integral component of Golgi membrane; cytoplasm; inositol phosphoceramide synthase complex; Golgi apparatus GO_0030173; GO_0005737; GO_0070916; GO_0005794 1458 YDR368W YPR1 NADPH-dependent aldo-keto reductase NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1459 YDR369C XRS2 Protein required for DNA repair Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling C IPI; IDA Mre11 complex; nucleus GO_0030870; GO_0005634 1460 YDR370C DXO1 mRNA 5'-end-capping quality-control protein mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p; C HDA cytoplasm GO_0005737 1461 YDR371C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene CTS2/YDR371W; identified by expression profiling and mass spectrometry 1462 YDR371W CTS2 Putative chitinase Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect C HDA cytoplasm GO_0005737 1463 YDR372C VPS74 Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 C IDA; IDA; IDA; IDA; HDA; HDA extrinsic component of membrane; Golgi medial cisterna; cytosol; Golgi apparatus; cytoplasm; nucleus GO_0019898; GO_0005797; GO_0005829; GO_0005794; GO_0005737; GO_0005634 1464 YDR373W FRQ1 N-myristoylated calcium-binding protein N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily C HDA; IDA cytosol; Golgi membrane GO_0005829; GO_0000139 1465 YDR374C PHO92 Posttranscriptional regulator of phosphate metabolism Posttranscriptional regulator of phosphate metabolism; facilitates PHO4 mRNA degradation by interacting with Pop2p; regulates PHO4 mRNA stability by binding to PHO4's 3'UTR in a phosphate-dependent manner; contains highly conserved YTH (YT521-B Homology) domain that exhibits RNA-binding activity; functional homolog of human YTHDF2 C IDA cytoplasm GO_0005737 1466 YDR374W-A WIP1 Kinetochore localized protein of unknown function Kinetochore localized protein of unknown function; interacts with Cnn1p (CENP-T); orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-W and fission yeast new1 C IDA kinetochore GO_0000776 1467 YDR375C BCS1 Protein translocase and chaperone required for Complex III assembly Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 1468 YDR376W ARH1 Oxidoreductase of the mitochondrial inner membrane Oxidoreductase of the mitochondrial inner membrane; involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 1469 YDR377W ATP17 Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis C IMP; HDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion GO_0000276; GO_0005739 1470 YDR378C LSM6 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA C IDA; IDA; IDA; IDA; IDA; IPI nucleolus; U4/U6 x U5 tri-snRNP complex; Lsm1-7-Pat1 complex; P-body; U6 snRNP; small nucleolar ribonucleoprotein complex GO_0005730; GO_0046540; GO_1990726; GO_0000932; GO_0005688; GO_0005732 1471 YDR379C-A SDH6 Mitochondrial protein involved in assembly of succinate dehydrogenase Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; member of the LYR protein family; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy C HDA mitochondrion GO_0005739 1472 YDR379W RGA2 GTPase-activating protein for polarity-establishment protein Cdc42p GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication C IDA cellular bud neck split septin rings GO_0032177 1473 YDR380W ARO10 Phenylpyruvate decarboxylase Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism C HDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 1474 YDR381C-A COI1 Mitochondrial assembly factor Protein of unknown function; localized to the mitochondrial outer membrane C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 1475 YDR381W YRA1 Nuclear polyadenylated RNA-binding protein Nuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member C IDA transcription export complex GO_0000346 1476 YDR382W RPP2B Ribosomal protein P2 beta Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm C HDA; IDA fungal-type vacuole; cytosolic large ribosomal subunit GO_0000324; GO_0022625 1477 YDR383C NKP1 Central kinetochore protein and subunit of the Ctf19 complex Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 C IDA kinetochore GO_0000776 1478 YDR384C ATO3 Plasma membrane protein, putative ammonium transporter Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters C HDA; HDA; IDA mitochondrion; cell periphery; plasma membrane GO_0005739; GO_0071944; GO_0005886 1479 YDR385W EFT2 Elongation factor 2 (EF-2), also encoded by EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication C IDA; HDA ribosome; cytosol GO_0005840; GO_0005829 1480 YDR386W MUS81 Subunit of structure-specific Mms4p-Mus81p endonuclease Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p C IDA; IPI Holliday junction resolvase complex; nucleus GO_0048476; GO_0005634 1481 YDR387C CIN10 Putative transporter Putative transporter; member of the sugar porter family; YDR387C is not an essential gene C HDA fungal-type vacuole membrane GO_0000329 1482 YDR388W RVS167 Calmodulin-binding actin-associated protein Actin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin C IDA; HDA; HDA; IPI; HDA actin cortical patch; mating projection tip; cytosol; Rvs161p-Rvs167p complex; cytoplasm GO_0030479; GO_0043332; GO_0005829; GO_1990528; GO_0005737 1483 YDR389W SAC7 GTPase activating protein (GAP) for Rho1p GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication C IDA cell cortex GO_0005938 1484 YDR390C UBA2 Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; C IDA; IPI nucleus; SUMO activating enzyme complex GO_0005634; GO_0031510 1485 YDR391C YDR391C Putative protein of unknown function Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1486 YDR392W SPT3 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA SLIK (SAGA-like) complex; cytosol; nucleus; SAGA complex GO_0046695; GO_0005829; GO_0005634; GO_0000124 1487 YDR393W SHE9 Protein required for normal mitochondrial morphology Protein required for normal mitochondrial morphology; mitochondrial inner membrane protein; may be involved in fission of the inner membrane; forms a homo-oligomeric complex C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 1488 YDR394W RPT3 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B C IDA proteasome regulatory particle, base subcomplex GO_0008540 1489 YDR395W SXM1 Nuclear transport factor (karyopherin) Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1490 YDR396W Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex 1491 YDR397C NCB2 Subunit of a heterodimeric NC2 transcription regulator complex Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p C IDA; IDA negative cofactor 2 complex; nucleus GO_0017054; GO_0005634 1492 YDR398W UTP5 Subunit of U3-containing Small Subunit (SSU) processome complex Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit C IDA; IDA; IDA; IDA rDNA heterochromatin; small-subunit processome; t-UTP complex; nucleolus GO_0033553; GO_0032040; GO_0034455; GO_0005730 1493 YDR399W HPT1 Dimeric hypoxanthine-guanine phosphoribosyltransferase Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1494 YDR400W URH1 Uridine nucleosidase (uridine-cytidine N-ribohydrolase) Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1495 YDR401W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1496 YDR402C DIT2 N-formyltyrosine oxidase N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s 1497 YDR403W DIT1 Sporulation-specific enzyme required for spore wall maturation Sporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure 1498 YDR404C RPB7 RNA polymerase II subunit B16 RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation C IDA; IDA; IDA; IDA DNA-directed RNA polymerase II, core complex; nucleus; P-body; cytoplasm GO_0005665; GO_0005634; GO_0000932; GO_0005737 1499 YDR405W MRP20 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1500 YDR406W PDR15 Plasma membrane ATP binding cassette (ABC) transporter Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication C HDA cell periphery GO_0071944 1501 YDR406W-A Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR 1502 YDR407C TRS120 Component of transport protein particle (TRAPP) complex II Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic C IDA; IDA; IDA TRAPPII protein complex; early endosome; trans-Golgi network GO_1990071; GO_0005769; GO_0005802 1503 YDR408C ADE8 Phosphoribosyl-glycinamide transformylase Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1504 YDR409W SIZ1 SUMO E3 ligase SUMO/Smt3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring; SIZ1 has a paralog, NFI1, that arose from the whole genome duplication C IDA; IDA septin ring; nuclear chromatin GO_0005940; GO_0000790 1505 YDR410C STE14 Farnesyl cysteine-carboxyl methyltransferase Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane C IDA; HDA; IMP endoplasmic reticulum membrane; endoplasmic reticulum; nuclear inner membrane GO_0005789; GO_0005783; GO_0005637 1506 YDR411C DFM1 Endoplasmic reticulum (ER) localized protein Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p C IDA; IDA; IDA endoplasmic reticulum; Doa10p ubiquitin ligase complex; Hrd1p ubiquitin ligase ERAD-L complex GO_0005783; GO_0000837; GO_0000839 1507 YDR412W RRP17 Component of the pre-60S pre-ribosomal particle Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA C IDA cytoplasm GO_0005737 1508 YDR413C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing 1509 YDR414C ERD1 Predicted membrane protein required for lumenal ER protein retention Predicted membrane protein required for lumenal ER protein retention; mutants secrete the endogenous ER protein, BiP (Kar2p) C IDA membrane GO_0016020 1510 YDR415C YDR415C Putative aminopeptidase Putative aminopeptidase C HDA fungal-type vacuole GO_0000324 1511 YDR416W SYF1 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans C IDA; IDA; IDA; IDA; IDA; IDA; IDA cytosol; nucleus; U2-type prespliceosome; Prp19 complex; U2-type post-mRNA release spliceosomal complex; U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome GO_0005829; GO_0005634; GO_0071004; GO_0000974; GO_0071008; GO_0071006; GO_0071007 1512 YDR417C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W 1513 YDR418W RPL12B Ribosomal 60S subunit protein L12B Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication C IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 1514 YDR419W RAD30 DNA polymerase eta DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV C IDA; IPI; IDA mitochondrion; replication fork; nucleus GO_0005739; GO_0005657; GO_0005634 1515 YDR420W HKR1 Mucin family member that functions as an osmosensor in the HOG pathway Mucin family member that functions as an osmosensor in the HOG pathway; functions in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection C IDA; IDA site of polarized growth; plasma membrane GO_0030427; GO_0005886 1516 YDR421W ARO80 Zinc finger transcriptional activator of the Zn2Cys6 family Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids C HDA nucleus GO_0005634 1517 YDR422C SIP1 Alternate beta-subunit of the Snf1p kinase complex Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions C IPI; IDA nucleotide-activated protein kinase complex; fungal-type vacuole GO_0031588; GO_0000324 1518 YDR423C CAD1 AP-1-like basic leucine zipper (bZIP) transcriptional activator AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1519 YDR424C DYN2 Cytoplasmic light chain dynein, microtubule motor protein Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments C IPI; IDA; IDA; IDA; IDA; HDA cytoplasmic dynein complex; peroxisome; cytoplasmic microtubule; nuclear pore; peroxisomal importomer complex; nuclear periphery GO_0005868; GO_0005777; GO_0005881; GO_0005643; GO_1990429; GO_0034399 1520 YDR425W SNX41 Sorting nexin Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p C IDA endosome GO_0005768 1521 YDR426C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 1522 YDR427W RPN9 Non-ATPase regulatory subunit of the 26S proteasome Non-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA proteasome storage granule; proteasome regulatory particle, lid subcomplex; cytosol; nucleus GO_0034515; GO_0008541; GO_0005829; GO_0005634 1523 YDR428C BNA7 Formylkynurenine formamidase Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine 1524 YDR429C TIF35 eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3) eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation C IDA; IDA; IDA multi-eIF complex; eukaryotic translation initiation factor 3 complex; cytoplasmic stress granule GO_0043614; GO_0005852; GO_0010494 1525 YDR430C CYM1 Lysine-specific metalloprotease of the pitrilysin family Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology C HDA; IDA mitochondrion; mitochondrial intermembrane space GO_0005739; GO_0005758 1526 YDR431W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1527 YDR432W NPL3 RNA-binding protein RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress C IDA; IMP; IDA nucleus; cytoplasm; cytoplasmic stress granule GO_0005634; GO_0005737; GO_0010494 1528 YDR433W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1529 YDR434W GPI17 Transmembrane protein Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog C IDA; IDA; IDA endoplasmic reticulum; integral component of membrane; GPI-anchor transamidase complex GO_0005783; GO_0016021; GO_0042765 1530 YDR435C PPM1 Carboxyl methyltransferase Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth 1531 YDR436W PPZ2 Serine/threonine protein phosphatase Z, isoform of Ppz1p Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance 1532 YDR437W GPI19 Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P C IDA; HDA; HDA; IPI integral component of endoplasmic reticulum membrane; nuclear envelope; endoplasmic reticulum; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO_0030176; GO_0005635; GO_0005783; GO_0000506 1533 YDR438W THI74 Mitochondrial transporter repressible by thiamine Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension C IDA; HDA mitochondrion; fungal-type vacuole GO_0005739; GO_0000324 1534 YDR439W LRS4 Nucleolar protein that forms a complex with Csm1p Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation C IDA; IDA; IDA nucleus; monopolin complex; nucleolus GO_0005634; GO_0033551; GO_0005730 1535 YDR440W DOT1 Nucleosomal histone H3-Lys79 methylase Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response C IDA nucleus GO_0005634 1536 YDR441C APT2 Potential adenine phosphoribosyltransferase Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1537 YDR442W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1538 YDR443C SSN2 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation C IDA mediator complex GO_0016592 1539 YDR444W YDR444W Putative hydrolase acting on ester bonds Putative hydrolase acting on ester bonds C HDA cytoplasm GO_0005737 1540 YDR445C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1541 YDR446W ECM11 Meiosis-specific protein Meiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure C IDA; IDA synaptonemal complex; nucleus GO_0000795; GO_0005634 1542 YDR447C RPS17B Ribosomal protein 51 (rp51) of the small (40s) subunit Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress 1543 YDR448W ADA2 Transcription coactivator Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes C IDA; IDA; IDA Ada2/Gcn5/Ada3 transcription activator complex; SLIK (SAGA-like) complex; SAGA complex GO_0005671; GO_0046695; GO_0000124 1544 YDR449C UTP6 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C IDA; IDA; IDA; HDA Pwp2p-containing subcomplex of 90S preribosome; small-subunit processome; nucleolus; 90S preribosome GO_0034388; GO_0032040; GO_0005730; GO_0030686 1545 YDR450W RPS18A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 1546 YDR451C YHP1 Homeobox transcriptional repressor Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication C IDA nuclear chromatin GO_0000790 1547 YDR452W PPN1 Dual endo- and exopolyphosphatase with a role in phosphate metabolism Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress C HDA; IDA; IMP; IDA fungal-type vacuole; fungal-type vacuole membrane; nucleus; cytosol GO_0000324; GO_0000329; GO_0005634; GO_0005829 1548 YDR453C TSA2 Stress inducible cytoplasmic thioredoxin peroxidase Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 1549 YDR454C GUK1 Guanylate kinase Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1550 YDR455C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W 1551 YDR456W NHX1 Na+/H+ and K+/H+ exchanger Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism C IDA; IDA; IDA; HDA; IDA late endosome; early endosome; trans-Golgi network; endoplasmic reticulum; fungal-type vacuole membrane GO_0005770; GO_0005769; GO_0005802; GO_0005783; GO_0000329 1552 YDR457W TOM1 E3 ubiquitin ligase of the hect-domain class E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase C HDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 1553 YDR458C HEH2 Inner nuclear membrane (INM) protein Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication C IDA; HDA nuclear envelope; nuclear periphery GO_0005635; GO_0034399 1554 YDR459C PFA5 Palmitoyltransferase with autoacylation activity Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 1555 YDR460W TFB3 Subunit of TFIIH and nucleotide excision repair factor 3 complexes Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit C IDA; IDA holo TFIIH complex; TFIIK complex GO_0005675; GO_0070985 1556 YDR461C-A CMI8 Putative protein of unknown function Putative protein of unknown function 1557 YDR461W MFA1 Mating pheromone a-factor Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 C IDA; HDA extracellular region; endoplasmic reticulum GO_0005576; GO_0005783 1558 YDR462W MRPL28 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1559 YDR463W STP1 Transcription factor Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; plasma membrane; cytoplasm GO_0005634; GO_0005886; GO_0005737 1560 YDR464C-A Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1561 YDR464W SPP41 Protein of unknown function Protein of unknown function; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia C IDA; HDA cytosol; nucleus GO_0005829; GO_0005634 1562 YDR465C RMT2 Arginine N5 methyltransferase Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1563 YDR466W PKH3 Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant 1564 YDR467C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1565 YDR468C TLG1 Essential t-SNARE that mediates fusion of vesicles with the late Golgi Essential t-SNARE that mediates fusion of vesicles with the late Golgi; forms a complex with Tlg2p and Vti1p; mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p C IPI; IDA; IDA SNARE complex; trans-Golgi network; endosome GO_0031201; GO_0005802; GO_0005768 1566 YDR469W SDC1 Subunit of the COMPASS (Set1C) complex Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 C IPI Set1C/COMPASS complex GO_0048188 1567 YDR470C UGO1 Outer membrane component of the mitochondrial fusion machinery Outer membrane component of the mitochondrial fusion machinery; binds directly to Fzo1p and Mgm1p and thus links these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but is not likely to function as a transmembrane transporter C HDA; HDA; IDA mitochondrion; mitochondrial outer membrane; integral component of mitochondrial outer membrane GO_0005739; GO_0005741; GO_0031307 1568 YDR471W RPL27B Ribosomal 60S subunit protein L27B Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication C IDA preribosome, large subunit precursor GO_0030687 1569 YDR472W TRS31 Core component of transport protein particle (TRAPP) complexes I-III Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) C IDA; IDA; IDA; HDA TRAPPI protein complex; TRAPPIII protein complex; TRAPPII protein complex; cytosol GO_1990070; GO_1990072; GO_1990071; GO_0005829 1570 YDR473C PRP3 Splicing factor Splicing factor; component of the U4/U6-U5 snRNP complex C IDA U4/U6 x U5 tri-snRNP complex GO_0046540 1571 YDR475C JIP4 Protein of unknown function Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication 1572 YDR476C YDR476C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene C HDA endoplasmic reticulum GO_0005783 1573 YDR477W SNF1 AMP-activated S/T protein kinase AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ubiquitin ligase C HDA; IPI; IPI; IDA; IPI; IPI mitochondrion; nucleotide-activated protein kinase complex; nucleus; nuclear envelope lumen; fungal-type vacuole; cytoplasm GO_0005739; GO_0031588; GO_0005634; GO_0005641; GO_0000324; GO_0005737 1574 YDR478W SNM1 Ribonuclease MRP complex subunit Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP C IPI ribonuclease MRP complex GO_0000172 1575 YDR479C PEX29 ER-resident protein involved in peroxisomal biogenesis Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress C IDA; IDA; IDA peroxisomal membrane; endoplasmic reticulum; peroxisome GO_0005778; GO_0005783; GO_0005777 1576 YDR480W DIG2 MAP kinase-responsive inhibitor of the Ste12p transcription factor MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication C IDA; IDA nucleus; Ste12p-Dig1p-Dig2p complex GO_0005634; GO_1990526 1577 YDR481C PHO8 Repressible vacuolar alkaline phosphatase Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN C IDA fungal-type vacuole membrane GO_0000329 1578 YDR482C CWC21 Protein involved in RNA splicing by the spliceosome Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p C IDA U2-type spliceosomal complex GO_0005684 1579 YDR483W KRE2 Alpha1,2-mannosyltransferase of the Golgi Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication C IDA; HDA Golgi apparatus; fungal-type vacuole membrane GO_0005794; GO_0000329 1580 YDR484W VPS52 Component of the GARP (Golgi-associated retrograde protein) complex Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p C IDA; IPI; HDA Golgi apparatus; GARP complex; cytosol GO_0005794; GO_0000938; GO_0005829 1581 YDR485C VPS72 Htz1p-binding component of the SWR1 complex Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting C HDA; IDA; HDA cytoplasm; Swr1 complex; nucleus GO_0005737; GO_0000812; GO_0005634 1582 YDR486C VPS60 Protein involved in late endosome to vacuole transport Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p C HDA; HDA fungal-type vacuole membrane; cytoplasm GO_0000329; GO_0005737 1583 YDR487C RIB3 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase) 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase); required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration C IDA; IDA cytosol; mitochondrial intermembrane space GO_0005829; GO_0005758 1584 YDR488C PAC11 Dynein intermediate chain, microtubule motor protein Dynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8 C IDA; IPI cytoplasmic microtubule; cytoplasmic dynein complex GO_0005881; GO_0005868 1585 YDR489W SLD5 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery C IMP; IPI; IDA; IDA DNA replication preinitiation complex; GINS complex; CMG complex; replication fork protection complex GO_0031261; GO_0000811; GO_0071162; GO_0031298 1586 YDR490C PKH1 Serine/threonine protein kinase Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication C IDA; IDA; IDA cell cortex; cytosol; eisosome GO_0005938; GO_0005829; GO_0032126 1587 YDR491C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1588 YDR492W IZH1 Membrane protein involved in zinc ion homeostasis Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 1589 YDR493W MZM1 Protein required for assembly of the cytochrome bc(1) complex Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog C IDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 1590 YDR494W RSM28 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1591 YDR495C VPS3 Component of CORVET membrane tethering complex Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase C IDA; IDA endosome; CORVET complex GO_0005768; GO_0033263 1592 YDR496C PUF6 Pumilio-homology domain protein Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis C IDA; IDA; HDA; HDA large ribosomal subunit; preribosome, large subunit precursor; nucleolus; nucleus GO_0015934; GO_0030687; GO_0005730; GO_0005634 1593 YDR497C ITR1 Myo-inositol transporter Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication C HDA; HDA; HDA; HDA fungal-type vacuole; fungal-type vacuole membrane; plasma membrane; cell periphery GO_0000324; GO_0000329; GO_0005886; GO_0071944 1594 YDR498C SEC20 Membrane glycoprotein v-SNARE Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p C IDA; IDA; IDA; IDA endoplasmic reticulum; SNARE complex; peroxisome; integral component of membrane GO_0005783; GO_0031201; GO_0005777; GO_0016021 1595 YDR499W LCD1 Essential protein required for the DNA integrity checkpoint pathways Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress C IDA; HDA nuclear chromosome; nucleus GO_0000228; GO_0005634 1596 YDR500C RPL37B Ribosomal 60S subunit protein L37B Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication C IDA; IDA preribosome, large subunit precursor; cytosolic large ribosomal subunit GO_0030687; GO_0022625 1597 YDR501W PLM2 Putative transcription factor, contains Forkhead Associated domain Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication C IDA nuclear chromatin GO_0000790 1598 YDR502C SAM2 S-adenosylmethionine synthetase S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication C IDA cytoplasmic stress granule GO_0010494 1599 YDR503C LPP1 Lipid phosphate phosphatase Lipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA C IDA membrane GO_0016020 1600 YDR504C SPG3 Protein required for high temperature survival during stationary phase Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources C HDA endoplasmic reticulum GO_0005783 1601 YDR505C PSP1 Asn and gln rich protein of unknown function Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication C HDA; IDA; HDA cytoplasm; P-body; mitochondrion GO_0005737; GO_0000932; GO_0005739 1602 YDR506C GMC1 Protein involved in meiotic progression Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein 1603 YDR507C GIN4 Protein kinase involved in bud growth and assembly of the septin ring Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication C IDA cellular bud neck GO_0005935 1604 YDR508C GNP1 High-affinity glutamine permease High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication C HDA; HDA; HDA; HDA cellular bud neck; cell periphery; mitochondrion; endoplasmic reticulum GO_0005935; GO_0071944; GO_0005739; GO_0005783 1605 YDR509W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1606 YDR510C-A Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1607 YDR510W SMT3 Ubiquitin-like protein of the SUMO family Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 C IDA; IDA; IDA nucleus; septin ring; condensed nuclear chromosome GO_0005634; GO_0005940; GO_0000794 1608 YDR511W SDH7 Mitochondrial protein involved in assembly of succinate dehydrogenase Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family C HDA; IDA mitochondrion; mitochondrial intermembrane space GO_0005739; GO_0005758 1609 YDR512C EMI1 Non-essential protein of unknown function Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs 1610 YDR513W GRX2 Cytoplasmic glutaredoxin Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication C IDA; HDA; IDA mitochondrion; nucleus; cytosol GO_0005739; GO_0005634; GO_0005829 1611 YDR514C YDR514C Protein of unknown function that localizes to mitochondria Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication C HDA; HDA mitochondrion; nucleus GO_0005739; GO_0005634 1612 YDR515W SLF1 RNA binding protein that associates with polysomes RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA cytoplasmic stress granule; polysome; cytoplasm GO_0010494; GO_0005844; GO_0005737 1613 YDR516C EMI2 Non-essential protein of unknown function Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 1614 YDR517W GRH1 Acetylated cis-Golgi protein, homolog of human GRASP65 Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress C IDA; IMP; IDA; HDA; IDA Golgi membrane; cis-Golgi network; cytoplasm; cytosol; COPII vesicle coat GO_0000139; GO_0005801; GO_0005737; GO_0005829; GO_0030127 1615 YDR518W EUG1 Protein disulfide isomerase of the endoplasmic reticulum lumen Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER C HDA endoplasmic reticulum GO_0005783 1616 YDR519W FPR2 Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase) Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress C HDA; HDA; HDA cytoplasm; nucleus; fungal-type vacuole GO_0005737; GO_0005634; GO_0000324 1617 YDR520C URC2 Putative Zn(II)2Cys6 motif containing transcription factor Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1618 YDR521W Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol 1619 YDR522C SPS2 Protein expressed during sporulation Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component C IDA; HDA fungal-type cell wall; plasma membrane GO_0009277; GO_0005886 1620 YDR523C SPS1 Putative protein serine/threonine kinase Putative protein serine/threonine kinase; expressed at the end of meiosis and localized to the prospore membrane; required for correct localization of enzymes involved in spore wall synthesis C HDA; IDA; IDA cytoplasm; prospore membrane; nucleus GO_0005737; GO_0005628; GO_0005634 1621 YDR524C AGE1 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif C IDA; IDA; HDA trans-Golgi network; endosome; cytoplasm GO_0005802; GO_0005768; GO_0005737 1622 YDR524C-A Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1623 YDR524C-B YDR524C-B Putative protein of unknown function Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 1624 YDR524W-C YDR524W-C Putative protein of unknown function Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin 1625 YDR525W API2 Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology 1626 YDR525W-A SNA2 Protein of unknown function Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C HDA; HDA cytoplasm; fungal-type vacuole membrane GO_0005737; GO_0000329 1627 YDR526C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1628 YDR527W RBA50 Protein involved in transcription Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 C HDA cytoplasm GO_0005737 1629 YDR528W HLR1 Protein involved in regulation of cell wall composition and integrity Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1630 YDR529C QCR7 Subunit 7 of ubiquinol cytochrome-c reductase (Complex III) Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly C HDA; IDA mitochondrion; mitochondrial respiratory chain complex III GO_0005739; GO_0005750 1631 YDR530C APA2 Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1632 YDR531W CAB1 Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1633 YDR532C KRE28 Subunit of a kinetochore-microtubule binding complex Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation C HDA; IDA; IDA spindle pole body; chromosome, centromeric region; condensed nuclear chromosome kinetochore GO_0005816; GO_0000775; GO_0000778 1634 YDR533C HSP31 Methylglyoxalase that converts methylglyoxal to D-lactate Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress C IDA; IDA cytoplasmic stress granule; P-body GO_0010494; GO_0000932 1635 YDR534C FIT1 Mannoprotein that is incorporated into the cell wall Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 1636 YDR535C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene 1637 YDR536W STL1 Glycerol proton symporter of the plasma membrane Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock C HDA; IDA; HDA cell periphery; plasma membrane; fungal-type vacuole GO_0071944; GO_0005886; GO_0000324 1638 YDR537C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF PAD1/YDR538W 1639 YDR538W PAD1 Phenylacrylic acid decarboxylase Phenylacrylic acid decarboxylase; confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX C IDA mitochondrion GO_0005739 1640 YDR539W FDC1 Ferulic acid decarboxylase Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 1641 YDR540C IRC4 Protein of unknown function Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1642 YDR541C YDR541C Aldehyde reductase Putative dihydrokaempferol 4-reductase 1643 YDR542W PAU10 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions C HDA fungal-type vacuole GO_0000324 1644 YDR543C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1645 YDR544C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1646 YDR545C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-1/YDR545W 1647 YDR545W YRF1-1 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 1648 YEL001C IRC22 Protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci C HDA endoplasmic reticulum GO_0005783 1649 YEL002C WBP1 Beta subunit of the oligosaccharyl transferase glycoprotein complex Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum C IPI; IDA; IDA; IMP oligosaccharyltransferase complex; endoplasmic reticulum; nuclear envelope; endoplasmic reticulum membrane GO_0008250; GO_0005783; GO_0005635; GO_0005789 1650 YEL003W GIM4 Subunit of the heterohexameric cochaperone prefoldin complex Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it C IPI; IDA prefoldin complex; cytoplasm GO_0016272; GO_0005737 1651 YEL004W YEA4 Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER C IDA endoplasmic reticulum GO_0005783 1652 YEL005C VAB2 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern C IDA; HDA BLOC-1 complex; cytoplasm GO_0031083; GO_0005737 1653 YEL006W YEA6 Putative mitochondrial NAD+ transporter Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 1654 YEL007W MIT1 Transcriptional regulator of pseudohyphal growth Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1655 YEL008C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1656 YEL008W YEL008W Putative protein of unknown function Hypothetical protein predicted to be involved in metabolism 1657 YEL009C GCN4 bZIP transcriptional activator of amino acid biosynthetic genes bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels C IDA nucleus GO_0005634 1658 YEL009C-A YEL009C-A Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YEL010W 1659 YEL010W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1660 YEL011W GLC3 Glycogen branching enzyme, involved in glycogen accumulation Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA cytoplasm GO_0005737 1661 YEL012W UBC8 Ubiquitin-conjugating enzyme that regulates gluconeogenesis Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro C IDA cytoplasm GO_0005737 1662 YEL013W VAC8 Vacuolar membrane protein Phosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions C HDA; IPI; IDA; IDA; IPI; HDA fungal-type vacuole membrane; nucleus-vacuole junction; integral component of membrane; membrane raft; Myo2p-Vac17p-Vac8p transport complex; cytosol GO_0000329; GO_0071561; GO_0016021; GO_0045121; GO_0071563; GO_0005829 1663 YEL014C YEL014C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1664 YEL015W EDC3 Non-essential conserved protein with a role in mRNA decapping Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress C HDA; IDA; IDA cytoplasm; nucleus; P-body GO_0005737; GO_0005634; GO_0000932 1665 YEL016C NPP2 Nucleotide pyrophosphatase/phosphodiesterase Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP2 has a paralog, NPP1, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants C HDA endoplasmic reticulum GO_0005783 1666 YEL017C-A PMP2 Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p) Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication C HDA; HDA; IDA fungal-type vacuole membrane; cell periphery; membrane GO_0000329; GO_0071944; GO_0016020 1667 YEL017W GTT3 Protein of unknown function may be involved in glutathione metabolism Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery C HDA nuclear periphery GO_0034399 1668 YEL018C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF EAF5/YEL018W 1669 YEL018W EAF5 Non-essential subunit of the NuA4 acetyltransferase complex Non-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia C HDA; IDA; IDA nucleus; cytosol; NuA4 histone acetyltransferase complex GO_0005634; GO_0005829; GO_0035267 1670 YEL019C MMS21 Highly conserved SUMO E3 ligase subunit of SMC5-SMC6 complex SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; required for efficient sister chromatid cohesion; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function C HDA; IDA; HDA; IDA cytoplasm; nuclear envelope; nucleus; Smc5-Smc6 complex GO_0005737; GO_0005635; GO_0005634; GO_0030915 1671 YEL020C PXP1 Peroxisomal matrix protein Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data C HDA; HDA peroxisome; cytoplasm GO_0005777; GO_0005737 1672 YEL020C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YEL020W-A; identified by fungal homology and RT-PCR 1673 YEL020W-A TIM9 Essential protein of the mitochondrial intermembrane space Essential protein of the mitochondrial intermembrane space; forms a complex with Tim10p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane C IDA; HDA; IDA mitochondrial intermembrane space protein transporter complex; mitochondrion; mitochondrial inner membrane protein insertion complex GO_0042719; GO_0005739; GO_0042721 1674 YEL021W URA3 Orotidine-5'-phosphate (OMP) decarboxylase Orotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound C IDA cytosol GO_0005829 1675 YEL022W GEA2 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication C IDA; IDA; HDA Golgi cis cisterna; extrinsic component of membrane; cytosol GO_0000137; GO_0019898; GO_0005829 1676 YEL023C YEL023C Putative protein of unknown function Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene 1677 YEL024W RIP1 Ubiquinol-cytochrome-c reductase Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly C HDA; IDA mitochondrion; mitochondrial respiratory chain complex III GO_0005739; GO_0005750 1678 YEL025C YEL025C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1679 YEL026W SNU13 RNA binding protein RNA binding protein; part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein C IDA; IDA; IDA; IDA nucleolus; box C/D snoRNP complex; U4/U6 x U5 tri-snRNP complex; small-subunit processome GO_0005730; GO_0031428; GO_0046540; GO_0032040 1680 YEL027W VMA3 Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis C IDA integral component of membrane GO_0016021 1681 YEL028W YEL028W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1682 YEL029C BUD16 Putative pyridoxal kinase Putative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) 1683 YEL030C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1684 YEL030W ECM10 Heat shock protein of the Hsp70 family Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 1685 YEL031W SPF1 P-type ATPase, ion transporter of the ER membrane P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 C HDA; IDA; HDA; IDA mitochondrion; endoplasmic reticulum membrane; endoplasmic reticulum; cis-Golgi network GO_0005739; GO_0005789; GO_0005783; GO_0005801 1686 YEL032C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1687 YEL032W MCM3 Protein involved in DNA replication Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex C IDA; HDA; IPI; IDA; IDA; IDA; IDA nucleus; cytoplasm; DNA replication preinitiation complex; replication fork protection complex; nuclear pre-replicative complex; CMG complex; MCM complex GO_0005634; GO_0005737; GO_0031261; GO_0031298; GO_0005656; GO_0071162; GO_0042555 1688 YEL033W MTC7 Protein of unknown function Protein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant 1689 YEL034C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps HYP2/YEL034W, a verified gene that encodes eiF-5A 1690 YEL034W HYP2 Translation elongation factor eIF-5A Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication C IPI; HDA; HDA cytosolic ribosome; mitochondrion; cytoplasm GO_0022626; GO_0005739; GO_0005737 1691 YEL035C UTR5 Protein of unknown function Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions 1692 YEL036C ANP1 Subunit of the alpha-1,6 mannosyltransferase complex Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol C IPI; IDA alpha-1,6-mannosyltransferase complex; Golgi cis cisterna GO_0000136; GO_0000137 1693 YEL037C RAD23 Protein with ubiquitin-like N terminus Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover C HDA; IMP; IDA mitochondrion; proteasome complex; nucleotide-excision repair factor 2 complex GO_0005739; GO_0000502; GO_0000111 1694 YEL038W UTR4 Protein with sequence similarity to acireductone synthases Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1695 YEL039C CYC7 Cytochrome c isoform 2, expressed under hypoxic conditions Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication C IDA; HDA mitochondrial intermembrane space; mitochondrion GO_0005758; GO_0005739 1696 YEL040W UTR2 Chitin transglycosylase Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck C IDA; IDA fungal-type cell wall; cellular bud neck septin ring GO_0009277; GO_0000144 1697 YEL041W YEF1 ATP-NADH kinase ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication 1698 YEL042W GDA1 Guanosine diphosphatase located in the Golgi Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate 1699 YEL043W YEL043W Predicted cytoskeleton protein involved in intracellular signaling Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold C HDA; HDA; HDA endoplasmic reticulum; cell periphery; ribosome GO_0005783; GO_0071944; GO_0005840 1700 YEL044W IES6 Component of the INO80 chromatin remodeling complex Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes C IPI; HDA Ino80 complex; nucleus GO_0031011; GO_0005634 1701 YEL045C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress 1702 YEL046C GLY1 Threonine aldolase Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis C IDA cytosol GO_0005829 1703 YEL047C FRD1 Soluble fumarate reductase Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication C HDA; HDA; HDA; IDA mitochondrion; ribosome; plasma membrane; cytosol GO_0005739; GO_0005840; GO_0005886; GO_0005829 1704 YEL048C TCA17 Component of transport protein particle (TRAPP) complex II Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder C IDA; IDA; HDA; HDA TRAPPII protein complex; Golgi apparatus; cytosol; clathrin-coated vesicle GO_1990071; GO_0005794; GO_0005829; GO_0030136 1705 YEL049W PAU2 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme 1706 YEL050C RML2 Mitochondrial ribosomal protein of the large subunit (L2) Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 1707 YEL050W-A Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR 1708 YEL051W VMA8 Subunit D of the V1 peripheral membrane domain of V-ATPase Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits C HDA fungal-type vacuole membrane GO_0000329 1709 YEL052W AFG1 Protein that may act as a chaperone for cytochrome c oxidase subunits Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 1710 YEL053C MAK10 Non-catalytic subunit of the NatC N-terminal acetyltransferase Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; expression is glucose-repressible C IDA; HDA NatC complex; cytoplasm GO_0031417; GO_0005737 1711 YEL053W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RPL12A/YEL054C 1712 YEL054C RPL12A Ribosomal 60S subunit protein L12A Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 1713 YEL055C POL5 DNA Polymerase phi DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA C IDA nucleolus GO_0005730 1714 YEL056W HAT2 Subunit of the Hat1p-Hat2p histone acetyltransferase complex Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing C IDA; IDA; IDA nucleus; cytoplasm; histone acetyltransferase complex GO_0005634; GO_0005737; GO_0000123 1715 YEL057C SDD1 Protein of unknown function Protein of unknown function involved in telomere maintenance; target of UME6 regulation 1716 YEL058W PCM1 Essential N-acetylglucosamine-phosphate mutase Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1717 YEL059C-A SOM1 Subunit of the mitochondrial inner membrane peptidase (IMP) Subunit of the mitochondrial inner membrane peptidase (IMP); IMP is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs C IPI; IDA mitochondrial inner membrane peptidase complex; mitochondrial inner membrane GO_0042720; GO_0005743 1718 YEL059W Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking 1719 YEL060C PRB1 Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication 1720 YEL061C CIN8 Kinesin motor protein Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation C IDA; HDA; IMP; IDA; IDA spindle microtubule; mitochondrion; astral microtubule; kinetochore microtubule; condensed nuclear chromosome kinetochore GO_0005876; GO_0005739; GO_0000235; GO_0005828; GO_0000778 1721 YEL062W NPR2 Subunit of the Iml1p/SEACIT complex Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human tumor suppressor NPRL2; target of Grr1p; required for growth on urea and proline C IDA; IDA; IDA Seh1-associated complex; GATOR1 complex; extrinsic component of fungal-type vacuolar membrane GO_0035859; GO_1990130; GO_0097042 1722 YEL063C CAN1 Plasma membrane arginine permease Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA membrane raft; endoplasmic reticulum; mitochondrion; plasma membrane GO_0045121; GO_0005783; GO_0005739; GO_0005886 1723 YEL064C AVT2 Putative transporter Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters C IDA endoplasmic reticulum GO_0005783 1724 YEL065W SIT1 Ferrioxamine B transporter Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p C IDA; IDA; HDA endosome; cytoplasmic vesicle; fungal-type vacuole GO_0005768; GO_0031410; GO_0000324 1725 YEL066W HPA3 D-Amino acid N-acetyltransferase that detoxifies D-amino acids D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1726 YEL067C YEL067C Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 1727 YEL068C YEL068C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels C HDA endoplasmic reticulum GO_0005783 1728 YEL069C HXT13 Putative transmembrane polyol transporter Hexose transporter; induced in the presence of non-fermentable carbon sources, induced by low levels of glucose, repressed by high levels of glucose; HXT13 has a paralog, HXT17, that arose from a segmental duplication C HDA endoplasmic reticulum GO_0005783 1729 YEL070W DSF1 Mannitol dehydrogenase Putative mannitol dehydrogenase; deletion suppressor of mpt5 mutation; DSF1 has a paralog, YNR073C, that arose from a segmental duplication 1730 YEL071W DLD3 2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase D-lactate dehydrogenase; part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm C IDA cytoplasm GO_0005737 1731 YEL072W RMD6 Protein required for sporulation Protein required for sporulation 1732 YEL073C YEL073C Putative protein of unknown function Putative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline 1733 YEL074W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large scale expression analysis showed an increase in mRNA accumulation in strains mutant for exosome components 1734 YEL075C YEL075C Putative protein of unknown function Putative protein of unknown function 1735 YEL075W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1736 YEL076C YEL076C Putative protein of unknown function Putative protein of unknown function 1737 YEL076C-A YEL076C-A Putative protein of unknown function Putative protein of unknown function 1738 YEL077C YEL077C Helicase-like protein encoded within the telomeric Y' element Helicase-like protein encoded within the telomeric Y' element 1739 YEL077W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1740 YER001W MNN1 Alpha-1,3-mannosyltransferase Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family C IDA Golgi apparatus GO_0005794 1741 YER002W NOP16 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis C HDA; IDA; IDA nucleus; preribosome, large subunit precursor; nucleolus GO_0005634; GO_0030687; GO_0005730 1742 YER003C PMI40 Mannose-6-phosphate isomerase Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1743 YER004W FMP52 Protein of unknown function Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation C HDA; HDA; HDA mitochondrion; endoplasmic reticulum; mitochondrial outer membrane GO_0005739; GO_0005783; GO_0005741 1744 YER005W YND1 Apyrase with wide substrate specificity Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity C HDA; HDA; IDA; IDA Golgi apparatus; COPI-coated vesicle; membrane; Golgi membrane GO_0005794; GO_0030137; GO_0016020; GO_0000139 1745 YER006C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PAC2/YER007W 1746 YER006W NUG1 GTPase that associates with nuclear 60S pre-ribosomes GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus C HDA; IGI; IDA nucleus; preribosome, large subunit precursor; nucleolus GO_0005634; GO_0030687; GO_0005730 1747 YER007C-A TMA20 Protein of unknown function that associates with ribosomes Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA ribosome; cytosol; cytoplasm GO_0005840; GO_0005829; GO_0005737 1748 YER007W PAC2 Microtubule effector required for tubulin heterodimer formation Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl 1749 YER008C SEC3 Subunit of the exocyst complex Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress C HDA; IDA; IDA; IDA; IDA; HDA; HDA mating projection tip; cellular bud tip; incipient cellular bud site; cellular bud neck; exocyst; prospore membrane; cytoplasm GO_0043332; GO_0005934; GO_0000131; GO_0005935; GO_0000145; GO_0005628; GO_0005737 1750 YER009W NTF2 Nuclear envelope protein Nuclear envelope protein; interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport C IDA nuclear envelope GO_0005635 1751 YER010C YER010C Bifunctional HMG aldolase/oxaloacetate decarboxylase Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate 1752 YER011W TIR1 Cell wall mannoprotein Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins C HDA; IDA; HDA cell periphery; fungal-type cell wall; fungal-type vacuole GO_0071944; GO_0009277; GO_0000324 1753 YER012W PRE1 Beta 4 subunit of the 20S proteasome Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle C IDA; IDA; IDA proteasome core complex, beta-subunit complex; nucleus; endoplasmic reticulum membrane GO_0019774; GO_0005634; GO_0005789 1754 YER013W PRP22 DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction C IMP; IMP U2-type post-spliceosomal complex; U2-type catalytic step 2 spliceosome GO_0071021; GO_0071007 1755 YER014C-A BUD25 Protein involved in bud-site selection Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern 1756 YER014W HEM14 Protoporphyrinogen oxidase Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 1757 YER015W FAA2 Medium chain fatty acyl-CoA synthetase Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome C IDA; HDA peroxisome; mitochondrion GO_0005777; GO_0005739 1758 YER016W BIM1 Microtubule plus end-tracking protein Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally C IDA; IDA; IDA; IDA; IDA; IDA spindle pole; microtubule plus-end; spindle; cytoplasmic microtubule; spindle midzone; spindle pole body GO_0000922; GO_0035371; GO_0005819; GO_0005881; GO_0051233; GO_0005816 1759 YER017C AFG3 Mitochondrial inner membrane m-AAA protease component Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging C IDA; HDA; IDA; IDA; IDA mitochondrial inner membrane; mitochondrion; mitochondrial inner boundary membrane; fungal-type vacuole membrane; m-AAA complex GO_0005743; GO_0005739; GO_0097002; GO_0000329; GO_0005745 1760 YER018C SPC25 Component of the kinetochore-associated Ndc80 complex Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p C IPI; IDA; IPI condensed nuclear chromosome kinetochore; Ndc80 complex; condensed nuclear chromosome, centromeric region GO_0000778; GO_0031262; GO_0000780 1761 YER019C-A SBH2 Ssh1p-Sss1p-Sbh2p complex component Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication C IDA Ssh1 translocon complex GO_0071261 1762 YER019W ISC1 Inositol phosphosphingolipid phospholipase C Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance C IDA; HDA; IDA; IDA mitochondrion; cell periphery; endoplasmic reticulum; integral component of mitochondrial outer membrane GO_0005739; GO_0071944; GO_0005783; GO_0031307 1763 YER020W GPA2 Nucleotide binding alpha subunit of the heterotrimeric G protein Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus C HDA; IDA; HDA mitochondrion; plasma membrane; cytosol GO_0005739; GO_0005886; GO_0005829 1764 YER021W RPN3 Essential non-ATPase regulatory subunit of the 26S proteasome lid Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control C IDA proteasome regulatory particle, lid subcomplex GO_0008541 1765 YER022W SRB4 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination C IDA core mediator complex GO_0070847 1766 YER023C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene PRO3; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 1767 YER023W PRO3 Delta 1-pyrroline-5-carboxylate reductase Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis C IDA cytoplasm GO_0005737 1768 YER024W YAT2 Carnitine acetyltransferase Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane C HDA; IDA cytoplasm; cytosol GO_0005737; GO_0005829 1769 YER025W GCD11 Gamma subunit of the translation initiation factor eIF2 Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID) C IDA; IDA; IDA; IMP; HDA multi-eIF complex; eukaryotic 48S preinitiation complex; eukaryotic 43S preinitiation complex; eukaryotic translation initiation factor 2 complex; cytosol GO_0043614; GO_0033290; GO_0016282; GO_0005850; GO_0005829 1770 YER026C CHO1 Phosphatidylserine synthase Phosphatidylserine synthase; functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline C IDA; IDA; IDA mitochondrial outer membrane; endoplasmic reticulum; integral component of membrane GO_0005741; GO_0005783; GO_0016021 1771 YER027C GAL83 One of three possible beta-subunits of the Snf1 kinase complex One of three possible beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain; GAL83 has a paralog, SIP2, that arose from the whole genome duplication C IDA; IDA; IDA; IPI nucleus; cytoplasm; nuclear envelope lumen; nucleotide-activated protein kinase complex GO_0005634; GO_0005737; GO_0005641; GO_0031588 1772 YER028C MIG3 Transcriptional regulator Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genesenvironment C IDA nuclear chromatin GO_0000790 1773 YER029C SMB1 Core Sm protein Sm B Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B' C IDA; IDA; IDA; IDA U4/U6 x U5 tri-snRNP complex; U2-type prespliceosome; U5 snRNP; U1 snRNP GO_0046540; GO_0071004; GO_0005682; GO_0005685 1774 YER030W CHZ1 Histone chaperone for Htz1p/H2A-H2B dimer Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress C HDA nucleus GO_0005634 1775 YER031C YPT31 Rab family GTPase Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication C IDA; HDA; IDA; HDA; IDA trans-Golgi network; mitochondrion; Golgi apparatus; mitochondrial outer membrane; endosome GO_0005802; GO_0005739; GO_0005794; GO_0005741; GO_0005768 1776 YER032W FIR1 Protein involved in 3' mRNA processing Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate C HDA cellular bud neck GO_0005935 1777 YER033C ZRG8 Protein of unknown function Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency C IDA; HDA; IDA; HDA; IDA mating projection tip; mitochondrion; cellular bud; cytoplasm; cellular bud neck GO_0043332; GO_0005739; GO_0005933; GO_0005737; GO_0005935 1778 YER034W YER034W Protein of unknown function Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1779 YER035W EDC2 RNA-binding protein that directly activates mRNA decapping RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication C IDA; IDA; HDA nucleus; nucleolus; cytoplasm GO_0005634; GO_0005730; GO_0005737 1780 YER036C ARB1 ATPase of the ATP-binding cassette (ABC) family ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p C HDA; HDA; HDA; IDA cytoplasm; ribosome; preribosome, large subunit precursor; nucleus GO_0005737; GO_0005840; GO_0030687; GO_0005634 1781 YER037W PHM8 Lysophosphatidic acid (LPA) phosphatase, nucleotidase Lysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; PHM8 has a paralog, SDT1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1782 YER038C KRE29 Subunit of the SMC5-SMC6 complex Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance C IDA; HDA; HDA Smc5-Smc6 complex; cytoplasm; nucleus GO_0030915; GO_0005737; GO_0005634 1783 YER038W-A FMP49 Mitochondrial protein of unknown function Mitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C C HDA mitochondrion GO_0005739 1784 YER039C HVG1 Protein of unknown function Protein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 1785 YER039C-A YER039C-A Putative protein of unknown function Putative protein of unknown function; YER039C-A is not an essential gene 1786 YER040W GLN3 Transcriptional activator of genes regulated by nitrogen catabolite r Transcriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 1787 YER041W YEN1 Holliday junction resolvase Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1788 YER042W MXR1 Methionine-S-sulfoxide reductase Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1789 YER043C SAH1 S-adenosyl-L-homocysteine hydrolase S-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels C HDA cytoplasm GO_0005737 1790 YER044C ERG28 Endoplasmic reticulum membrane protein Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p C HDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 1791 YER044C-A MEI4 Meiosis-specific protein involved in forming DSBs Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores C IDA condensed nuclear chromosome GO_0000794 1792 YER045C ACA1 ATF/CREB family basic leucine zipper (bZIP) transcription factor ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication 1793 YER046W SPO73 Meiosis-specific protein required for prospore membrane morphogenesis Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis C IDA; IDA cytosol; prospore membrane GO_0005829; GO_0005628 1794 YER046W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SAP1/YER047C 1795 YER047C SAP1 Putative ATPase of the AAA family Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system C HDA cytoplasm GO_0005737 1796 YER048C CAJ1 Nuclear type II J heat shock protein of the E. coli dnaJ family Nuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly 1797 YER048W-A ISD11 Cysteine desulfurase (Nfs1p) activator Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family C HDA; IDA; IDA; IPI mitochondrion; extrinsic component of mitochondrial inner membrane; mitochondrial matrix; L-cysteine desulfurase complex GO_0005739; GO_0031314; GO_0005759; GO_1990221 1798 YER049W TPA1 Fe(II)/2-oxoglutarate-dependent dioxygenase family member Poly(rA)-binding protein; involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(III) and 2-oxoglutarate C HDA nucleus GO_0005634 1799 YER050C RSM18 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 1800 YER051W JHD1 JmjC domain family histone demethylase specific for H3-K36 JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe 1801 YER052C HOM3 Aspartate kinase (L-aspartate 4-P-transferase) Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis C HDA cytoplasm GO_0005737 1802 YER053C PIC2 Mitochondrial copper and phosphate carrier Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature C IDA mitochondrion GO_0005739 1803 YER053C-A YER053C-A Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress C HDA endoplasmic reticulum GO_0005783 1804 YER054C GIP2 Putative regulatory subunit of protein phosphatase Glc7p Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication C IPI protein phosphatase type 1 complex GO_0000164 1805 YER055C HIS1 ATP phosphoribosyltransferase ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control 1806 YER056C FCY2 Purine-cytosine permease Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress C IDA; HDA; HDA plasma membrane; cell periphery; fungal-type vacuole membrane GO_0005886; GO_0071944; GO_0000329 1807 YER056C-A RPL34A Ribosomal 60S subunit protein L34A Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication C IDA preribosome, large subunit precursor GO_0030687 1808 YER057C HMF1 Member of the p14.5 protein family Member of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cytosol; nucleus; cytoplasm; mitochondrial intermembrane space GO_0005829; GO_0005634; GO_0005737; GO_0005758 1809 YER058W PET117 Assembly factor that couples heme a synthesis to complex IV assembly Protein required for assembly of cytochrome c oxidase C IDA; IDA; HDA extrinsic component of matrix side of mitochondrial inner membrane; mitochondrial matrix; mitochondrion GO_0099616; GO_0005759; GO_0005739 1810 YER059W PCL6 Pho85p cyclin of the Pho80p subfamily Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication 1811 YER060W FCY21 Putative purine-cytosine permease Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function 1812 YER060W-A FCY22 Putative purine-cytosine permease Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 1813 YER061C CEM1 Mitochondrial beta-keto-acyl synthase Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration C HDA mitochondrion GO_0005739 1814 YER062C GPP2 DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1815 YER063W THO1 Conserved nuclear RNA-binding protein Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation C HDA nucleus GO_0005634 1816 YER064C VHR2 Non-essential nuclear protein Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1817 YER065C ICL1 Isocitrate lyase Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose C IDA cytoplasm GO_0005737 1818 YER066C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YER067W 1819 YER066W RRT13 Putative protein of unknown function Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription 1820 YER067C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF RGI1/YER067W 1821 YER067W RGI1 Protein of unknown function Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication C HDA; HDA; IDA cytoplasm; nucleus; cell periphery GO_0005737; GO_0005634; GO_0071944 1822 YER068C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARG5,6/YER069W 1823 YER068W MOT2 Ubiquitin-protein ligase subunit of the CCR4-NOT complex Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication C IDA; IPI cytoplasm; CCR4-NOT core complex GO_0005737; GO_0030015 1824 YER069W ARG5,6 Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 1825 YER070W RNR1 Major isoform of large subunit of ribonucleotide-diphosphate reductase Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication C IDA; HDA; IDA cytoplasm; nucleus; ribonucleoside-diphosphate reductase complex GO_0005737; GO_0005634; GO_0005971 1826 YER071C TDA2 Subunit of a complex that associates with actin filaments Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele C HDA; IDA; HDA cytoplasm; actin cortical patch; mating projection tip GO_0005737; GO_0030479; GO_0043332 1827 YER072W VTC1 Regulatory subunit of the vacuolar transporter chaperone (VTC) complex Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress C IPI; IDA; HDA; IDA vacuolar transporter chaperone complex; intrinsic component of vacuolar membrane; fungal-type vacuole membrane; endoplasmic reticulum GO_0033254; GO_0031310; GO_0000329; GO_0005783 1828 YER073W ALD5 Mitochondrial aldehyde dehydrogenase Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed C HDA mitochondrion GO_0005739 1829 YER074W RPS24A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 1830 YER074W-A YOS1 Integral membrane protein required for ER to Golgi transport Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p C IDA; IPI; IPI integral component of Golgi membrane; endoplasmic reticulum membrane; ER to Golgi transport vesicle GO_0030173; GO_0005789; GO_0030134 1831 YER075C PTP3 Phosphotyrosine-specific protein phosphatase Phosphotyrosine-specific protein phosphatase; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1832 YER076C YER076C Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization C HDA; HDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 1833 YER076W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER076C 1834 YER077C MRX1 Protein that associates with mitochondrial ribosome Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport C HDA mitochondrion GO_0005739 1835 YER078C ICP55 Mitochondrial aminopeptidase Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family C HDA; IDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane; nucleus GO_0005739; GO_0031314; GO_0005634 1836 YER078W-A YER078W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 1837 YER079C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF AIM9/YER080W 1838 YER079W YER079W Putative protein of unknown function Putative protein of unknown function C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1839 YER080W AIM9 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 1840 YER081W SER3 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1841 YER082C UTP7 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C IDA; IDA; HDA nucleolus; small-subunit processome; 90S preribosome GO_0005730; GO_0032040; GO_0030686 1842 YER083C GET2 Subunit of the GET complex Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division C HDA; IGI endoplasmic reticulum; GET complex GO_0005783; GO_0043529 1843 YER084W YER084W Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels 1844 YER084W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps uncharacterized ORF YER085C 1845 YER085C YER085C Putative protein of unknown function Putative protein of unknown function 1846 YER086W ILV1 Threonine deaminase, catalyzes first step in isoleucine biosynthesis Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation C HDA mitochondrion GO_0005739 1847 YER087C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W 1848 YER087C-B SBH1 Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p) Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; cotranslationally N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose from the whole genome duplication C IDA; HDA Sec61 translocon complex; endoplasmic reticulum GO_0005784; GO_0005783 1849 YER087W AIM10 Protein with similarity to tRNA synthetases Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 1850 YER088C DOT6 Protein involved in rRNA and ribosome biogenesis Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; HDA nucleus; Rpd3L-Expanded complex; cytoplasm GO_0005634; GO_0070210; GO_0005737 1851 YER088C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs PTC2/YER089C and YER088W-B 1852 YER088W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ORF PTC2/YER089C; identified by gene-trapping, expression analysis, and homology searching 1853 YER089C PTC2 Type 2C protein phosphatase (PP2C) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1854 YER090C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1855 YER090W TRP2 Anthranilate synthase Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p C HDA; IPI cytoplasm; anthranilate synthase complex GO_0005737; GO_0005950 1856 YER091C MET6 Cobalamin-independent methionine synthase Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs C HDA; HDA cytoplasm; plasma membrane GO_0005737; GO_0005886 1857 YER091C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1858 YER092W IES5 Non-essential INO80 chromatin remodeling complex subunit Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination C HDA; IPI nucleus; Ino80 complex GO_0005634; GO_0031011 1859 YER093C TSC11 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p) Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p); TORC2 is a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain C HDA; IPI cytosol; TORC2 complex GO_0005829; GO_0031932 1860 YER093C-A AIM11 Protein of unknown function Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 1861 YER094C PUP3 Beta 3 subunit of the 20S proteasome Beta 3 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit C10 C IDA; HDA proteasome core complex, beta-subunit complex; nucleus GO_0019774; GO_0005634 1862 YER095W RAD51 Strand exchange protein Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein C IDA; IDA nuclear chromosome; condensed nuclear chromosome GO_0000228; GO_0000794 1863 YER096W SHC1 Sporulation-specific activator of Chs3p (chitin synthase III) Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication C IDA prospore membrane GO_0005628 1864 YER097W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 1865 YER098W UBP9 Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1866 YER099C PRS2 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication C IDA; HDA ribose phosphate diphosphokinase complex; cytoplasm GO_0002189; GO_0005737 1867 YER100W UBC6 Ubiquitin-conjugating enzyme involved in ERAD Ubiquitin-conjugating enzyme involved in ERAD; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; ER-associated protein degradation is also known as ERAD C HDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 1868 YER101C AST2 Lipid raft associated protein Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1869 YER102W RPS8B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8B has a paralog, RPS8A, that arose from the whole genome duplication C HDA; IDA 90S preribosome; cytosolic small ribosomal subunit GO_0030686; GO_0022627 1870 YER103W SSA4 Heat shock protein that is highly induced upon stress Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 1871 YER104W RTT105 Protein with a role in regulation of Ty1 transposition Protein with a role in regulation of Ty1 transposition C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1872 YER105C NUP157 Subunit of the inner ring of the nuclear pore complex (NPC) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication C IDA; IDA nuclear pore; nuclear pore inner ring GO_0005643; GO_0044611 1873 YER106W MAM1 Monopolin Monopolin; kinetochore associated protein involved in chromosome attachment to meiotic spindle C IDA; IDA condensed nuclear chromosome kinetochore; monopolin complex GO_0000778; GO_0033551 1874 YER107C GLE2 RNA export factor associated with the nuclear pore complex (NPC) RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 C IDA; IDA nuclear pore; cytoplasm GO_0005643; GO_0005737 1875 YER107W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF GLE2/YER107C 1876 YER109C FLO8 Transcription factor Transcription factor; required for flocculation, diploid filamentous growth, and haploid invasive growth; forms a heterodimer with Mss1p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1; genome reference strain S288C and most laboratory strains have a nonsense mutation in this gene C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1877 YER110C KAP123 Karyopherin beta Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 C IDA; IDA; IDA; IDA cytoplasm; nucleus; nuclear pore; cytoplasmic stress granule GO_0005737; GO_0005634; GO_0005643; GO_0010494 1878 YER111C SWI4 DNA binding component of the SBF complex (Swi4p-Swi6p) DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p C IPI; IDA SBF transcription complex; nuclear chromatin GO_0033309; GO_0000790 1879 YER112W LSM4 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; IDA; IDA; HDA; IDA; IPI; HDA U4/U6 x U5 tri-snRNP complex; nucleolus; U6 snRNP; P-body; cytoplasm; Lsm1-7-Pat1 complex; small nucleolar ribonucleoprotein complex; nucleus GO_0046540; GO_0005730; GO_0005688; GO_0000932; GO_0005737; GO_1990726; GO_0005732; GO_0005634 1880 YER113C TMN3 Protein with a role in cellular adhesion and filamentous growth Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation C HDA; HDA COPI-coated vesicle; Golgi apparatus GO_0030137; GO_0005794 1881 YER114C BOI2 Protein implicated in polar growth, functionally redundant with Boi1p Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication C IDA; IDA; IDA site of polarized growth; cellular bud neck; cellular bud GO_0030427; GO_0005935; GO_0005933 1882 YER115C SPR6 Protein of unknown function Protein of unknown function; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation C HDA mitochondrion GO_0005739 1883 YER116C SLX8 Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress C IDA; HDA; HDA; IDA SUMO-targeted ubiquitin ligase complex; nucleus; cytoplasm; condensed nuclear chromosome kinetochore GO_0033768; GO_0005634; GO_0005737; GO_0000778 1884 YER117W RPL23B Ribosomal 60S subunit protein L23B Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication C IDA; IDA; IDA cytosolic large ribosomal subunit; nucleus; cytosol GO_0022625; GO_0005634; GO_0005829 1885 YER118C SHO1 Transmembrane osmosensor for filamentous growth and HOG pathways Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p C IPI; HDA; IDA; IDA; HDA; HDA; IDA HICS complex; cell periphery; cellular bud; plasma membrane; endoplasmic reticulum; cellular bud neck; mating projection tip GO_0044697; GO_0071944; GO_0005933; GO_0005886; GO_0005783; GO_0005935; GO_0043332 1886 YER119C AVT6 Vacuolar aspartate and glutamate exporter Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication C IDA; IDA fungal-type vacuole; fungal-type vacuole membrane GO_0000324; GO_0000329 1887 YER119C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 1888 YER120W SCS2 Integral ER membrane protein, regulates phospholipid metabolism Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA; IDA; IPI; IDA nuclear envelope; endoplasmic reticulum; nuclear membrane; nucleus-vacuole junction; cellular bud tip; integral component of endoplasmic reticulum membrane; cellular bud neck; plasma membrane GO_0005635; GO_0005783; GO_0031965; GO_0071561; GO_0005934; GO_0030176; GO_0005935; GO_0005886 1889 YER121W YER121W Putative protein of unknown function Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy 1890 YER122C GLO3 ADP-ribosylation factor GTPase activating protein (ARF GAP) ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p C IPI; IPI; IDA COPI vesicle coat; endoplasmic reticulum-Golgi intermediate compartment; COPI-coated vesicle GO_0030126; GO_0005793; GO_0030137 1891 YER123W YCK3 Palmitoylated vacuolar membrane-localized casein kinase I isoform Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway C IDA; IDA; IDA; IDA fungal-type vacuole membrane; plasma membrane; nucleus; fungal-type vacuole GO_0000329; GO_0005886; GO_0005634; GO_0000324 1892 YER124C DSE1 Daughter cell-specific protein Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA cellular bud neck GO_0005935 1893 YER125W RSP5 NEDD4 family E3 ubiquitin ligase E3 ubiquitin ligase of NEDD4 family; regulates many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, ribosome stability; mutant tolerates aneuploidy; autoubiquitinates; ubiquitinates Sec23p and Sna3p; deubiquitinated by Ubp2p; activity regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; human homolog implicated in Liddle syndrome C HDA; HDA; IDA; IDA; IDA; IPI; IDA; IDA; IDA plasma membrane; mitochondrion; cellular bud tip; cytoplasm; endosome membrane; ubiquitin ligase complex; extrinsic component of cytoplasmic side of plasma membrane; nucleus; Golgi apparatus GO_0005886; GO_0005739; GO_0005934; GO_0005737; GO_0010008; GO_0000151; GO_0031234; GO_0005634; GO_0005794 1894 YER126C NSA2 Protein constituent of 66S pre-ribosomal particles Protein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA C HDA; IDA preribosome, large subunit precursor; nucleus GO_0030687; GO_0005634 1895 YER127W LCP5 Essential protein involved in maturation of 18S rRNA Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus 1896 YER128W VFA1 Protein that interacts with Vps4p and has a role in vacuolar sorting Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology C IDA; IDA cytoplasm; endosome GO_0005737; GO_0005768 1897 YER129W SAK1 Upstream serine/threonine kinase for the SNF1 complex Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 1898 YER130C COM2 Transcription factor that binds IME1 Upstream Activation Signal (UAS) Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under all growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress 1899 YER131W RPS26B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication 1900 YER132C PMD1 Protein with an N-terminal kelch-like domain Protein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 1901 YER133W GLC7 Type 1 S/T protein phosphatase (PP1) catalytic subunit Type 1 serine/threonine protein phosphatase catalytic subunit; cleavage and polyadenylation factor (CPF) component; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA mRNA cleavage and polyadenylation specificity factor complex; nucleus; cellular bud neck; nucleolus; condensed nuclear chromosome kinetochore; spindle pole body; protein phosphatase type 1 complex; cell division site; mating projection base GO_0005847; GO_0005634; GO_0005935; GO_0005730; GO_0000778; GO_0005816; GO_0000164; GO_0032153; GO_0001400 1902 YER133W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF YER134C 1903 YER134C YER134C Magnesium-dependent acid phosphatase Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1904 YER135C YER135C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YER135C is not an essential gene 1905 YER136W GDI1 GDP dissociation inhibitor GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins 1906 YER137C YER137C Putative protein of unknown function Putative protein of unknown function 1907 YER137C-A YER137C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1908 YER137W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YER137C 1909 YER138C YER138C Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1910 YER138W-A YER138W-A Putative protein of unknown function Putative protein of unknown function; YER138W-A has a paralog, YBL107W-A, that arose from a single-locus duplication 1911 YER139C RTR1 CTD phosphatase CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1912 YER140W EMP65 Integral membrane protein of the ER ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; HDA; HDA; HDA integral component of endoplasmic reticulum membrane; endoplasmic reticulum; cell periphery; mitochondrion GO_0030176; GO_0005783; GO_0071944; GO_0005739 1913 YER141W COX15 Heme a synthase Protein required for the hydroxylation of heme O to form heme A; heme A is an essential prosthetic group for cytochrome c oxidase C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 1914 YER142C MAG1 3-methyl-adenine DNA glycosylase 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress 1915 YER143W DDI1 DNA damage-inducible v-SNARE binding protein DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains C IDA plasma membrane GO_0005886 1916 YER144C UBP5 Putative ubiquitin-specific protease Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication C IDA; IDA cellular bud neck; incipient cellular bud site GO_0005935; GO_0000131 1917 YER145C FTR1 High affinity iron permease High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress C IDA; IDA high-affinity iron permease complex; plasma membrane GO_0033573; GO_0005886 1918 YER145C-A YER145C-A Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF LSM5/YER146W 1919 YER146W LSM5 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA C IDA; IPI; IDA; IDA; IDA U6 snRNP; small nucleolar ribonucleoprotein complex; Lsm1-7-Pat1 complex; U4/U6 x U5 tri-snRNP complex; nucleolus GO_0005688; GO_0005732; GO_1990726; GO_0046540; GO_0005730 1920 YER147C SCC4 Subunit of cohesin loading factor (Scc2p-Scc4p) Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX C IDA; IPI nuclear chromatin; SMC loading complex GO_0000790; GO_0032116 1921 YER147C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SPT15/YER148W 1922 YER148W SPT15 TATA-binding protein (TBP) TATA-binding protein; general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability C IDA; IDA; IDA; IDA; IDA RNA polymerase I core factor complex; transcription factor TFIID complex; RNA polymerase I upstream activating factor complex; nucleus; transcription factor TFIIIB complex GO_0070860; GO_0005669; GO_0000500; GO_0005634; GO_0000126 1923 YER148W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF PEA2/YER149C 1924 YER149C PEA2 Coiled-coil 12S polarisome subunit Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion C IDA; IDA; IDA; IDA; IDA; HDA cellular bud tip; incipient cellular bud site; polarisome; cellular bud; cellular bud neck; mating projection tip GO_0005934; GO_0000131; GO_0000133; GO_0005933; GO_0005935; GO_0043332 1925 YER150W SPI1 GPI-anchored cell wall protein involved in weak acid resistance GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication C IDA; HDA fungal-type cell wall; cell periphery GO_0009277; GO_0071944 1926 YER151C UBP3 Ubiquitin-specific protease involved in transport and osmotic response Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress; role in ribophagy C IPI; IDA; HDA Ubp3-Bre5 deubiquitination complex; cytoplasm; cytosol GO_1990861; GO_0005737; GO_0005829 1927 YER152C YER152C Protein with 2-aminoadipate transaminase activity Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1928 YER152W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YER152C 1929 YER153C PET122 Mitochondrial translational activator specific for the COX3 mRNA Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; integral component of mitochondrial inner membrane GO_0005739; GO_0005743; GO_0031305 1930 YER154W OXA1 Mitochondrial inner membrane insertase Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals C HDA; IPI; IDA; IDA mitochondrion; mitochondrial large ribosomal subunit; mitochondrial inner boundary membrane; mitochondrial crista GO_0005739; GO_0005762; GO_0097002; GO_0030061 1931 YER155C BEM2 Rho GTPase activating protein (RhoGAP) Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p C IDA; HDA; HDA; IDA; IDA; IDA; IDA; IDA incipient cellular bud site; mitochondrion; prospore membrane; plasma membrane; cell cortex; mating projection tip; cytoplasm; cellular bud tip GO_0000131; GO_0005739; GO_0005628; GO_0005886; GO_0005938; GO_0043332; GO_0005737; GO_0005934 1932 YER156C YER156C Putative protein of unknown function Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1933 YER157W COG3 Essential component of the conserved oligomeric Golgi complex Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C IMP Golgi transport complex GO_0017119 1934 YER158C YER158C Protein of unknown function Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication 1935 YER158W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1936 YER159C BUR6 Subunit of a heterodimeric NC2 transcription regulator complex Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p C IDA negative cofactor 2 complex GO_0017054 1937 YER159C-A YER159C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1938 YER160C YER160C Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1939 YER161C SPT2 Protein involved in negative regulation of transcription Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 1940 YER162C RAD4 Protein that recognizes and binds damaged DNA (with Rad23p) during NER Protein that recognizes and binds damaged DNA (with Rad23p) during NER; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins; NER stands for nucleotide excision repair C IDA; IDA; IDA nucleotide-excision repair factor 2 complex; nucleus; cytosol GO_0000111; GO_0005634; GO_0005829 1941 YER163C GCG1 Gamma-glutamyl cyclotransferase Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 1942 YER164W CHD1 Chromatin remodeler that regulates various aspects of transcription Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes C HDA; IDA; IDA; IDA; IDA mitochondrion; nucleolar chromatin; nuclear chromatin; SLIK (SAGA-like) complex; SAGA complex GO_0005739; GO_0030874; GO_0000790; GO_0046695; GO_0000124 1943 YER165C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DNF1/YER166W 1944 YER165W PAB1 Poly(A) binding protein Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex C HDA; IDA; IDA; IDA nucleus; cytoplasm; cytoplasmic stress granule; ribosome GO_0005634; GO_0005737; GO_0010494; GO_0005840 1945 YER166W DNF1 Aminophospholipid translocase (flippase) Aminophospholipid translocase (flippase); localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase; DNF1 has a paralog, DNF2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; HDA; HDA; IPI cellular bud neck; mating projection tip membrane; plasma membrane; mitochondrion; cell periphery; endoplasmic reticulum; Lem3p-Dnf1p complex GO_0005935; GO_0070867; GO_0005886; GO_0005739; GO_0071944; GO_0005783; GO_1990531 1946 YER167W BCK2 Serine/threonine-rich protein involved in PKC1 signaling pathway Serine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1947 YER168C CCA1 ATP (CTP):tRNA-specific tRNA nucleotidyltransferase ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 1948 YER169W RPH1 JmjC domain-containing histone demethylase JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNAP II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; target of stess-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication 1949 YER170W ADK2 Mitochondrial adenylate kinase Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background C IDA; HDA; IDA mitochondrial inner membrane; mitochondrion; mitochondrial matrix GO_0005743; GO_0005739; GO_0005759 1950 YER171W RAD3 5' to 3' DNA helicase 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA nucleotide-excision repair factor 3 complex; core TFIIH complex; holo TFIIH complex GO_0000112; GO_0000439; GO_0005675 1951 YER172C BRR2 RNA-dependent ATPase RNA helicase (DEIH box) RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD C IDA; IDA; HDA U5 snRNP; U4/U6 x U5 tri-snRNP complex; nucleus GO_0005682; GO_0046540; GO_0005634 1952 YER172C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAD24/YER173W 1953 YER173W RAD24 Checkpoint protein Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein C IPI; IDA nucleus; Rad17 RFC-like complex GO_0005634; GO_0031389 1954 YER174C GRX4 Glutathione-dependent oxidoreductase Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication C IGI nucleus GO_0005634 1955 YER175C TMT1 Trans-aconitate methyltransferase Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle C IDA cytosol GO_0005829 1956 YER175W-A YER175W-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1957 YER176W ECM32 DNA dependent ATPase/DNA helicase DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes C IDA; IDA polysome; cytoplasmic stress granule GO_0005844; GO_0010494 1958 YER177W BMH1 14-3-3 protein, major isoform 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication C HDA; IDA; HDA; IDA plasma membrane; nucleus; cytoplasm; cytoplasmic stress granule GO_0005886; GO_0005634; GO_0005737; GO_0010494 1959 YER178W PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes C IDA; IDA; IDA mitochondrion; mitochondrial pyruvate dehydrogenase complex; mitochondrial nucleoid GO_0005739; GO_0005967; GO_0042645 1960 YER179W DMC1 Meiosis-specific recombinase required for double-strand break repair Meiosis-specific recombinase required for repair of double-strand breaks; also required for pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p C IDA; HDA condensed nuclear chromosome; nucleus GO_0000794; GO_0005634 1961 YER180C ISC10 Protein required for sporulation Protein required for sporulation; transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells 1962 YER180C-A SLO1 Protein interacting with Arl3p Protein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO 1963 YER181C YER181C Mitochondrial protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 1964 YER182W FMP10 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 1965 YER183C FAU1 5,10-methenyltetrahydrofolate synthetase 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis C HDA mitochondrion GO_0005739 1966 YER184C TOG1 Transcriptional activator of oleate genes Transcriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source C HDA nucleus GO_0005634 1967 YER185W PUG1 Plasma membrane protein involved in protoprophyrin and heme transport Plasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins C IDA plasma membrane GO_0005886 1968 YER186C YER186C Putative protein of unknown function Putative protein of unknown function 1969 YER187W YER187W Putative protein of unknown function Putative protein of unknown function; induced in respiratory-deficient cells 1970 YER188C-A YER188C-A Putative protein of unknown function Putative protein of unknown function 1971 YER188W YER188W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p 1972 YER189W YER189W Putative protein of unknown function Putative protein of unknown function 1973 YER190C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-2/YER190W; identified by gene-trapping, expression analysis, and homology 1974 YER190C-B YER190C-B Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 1975 YER190W YRF1-2 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p; induced by treatment with 8-methoxypsoralen and UVA irradiation 1976 YFL001W DEG1 tRNA:pseudouridine synthase tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 1977 YFL002C SPB4 Putative ATP-dependent RNA helicase Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients C HDA; IPI; IDA; IDA nucleolus; 90S preribosome; nucleoplasm; preribosome, large subunit precursor GO_0005730; GO_0030686; GO_0005654; GO_0030687 1978 YFL002W-A YFL002W-A Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 1979 YFL002W-B YFL002W-B Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1980 YFL003C MSH4 Protein involved in meiotic recombination Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein C IDA nuclear chromosome GO_0000228 1981 YFL004W VTC2 Regulatory subunit of the vacuolar transporter chaperone (VTC) complex Subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication C IDA; HDA; IPI endoplasmic reticulum; fungal-type vacuole membrane; vacuolar transporter chaperone complex GO_0005783; GO_0000329; GO_0033254 1982 YFL005W SEC4 Rab family GTPase Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane C HDA; HDA; HDA; IDA; IDA; HDA; IDA; HDA; IDA mitochondrion; cellular bud neck; plasma membrane; incipient cellular bud site; vesicle; mitochondrial outer membrane; mating projection tip; endoplasmic reticulum; transport vesicle GO_0005739; GO_0005935; GO_0005886; GO_0000131; GO_0031982; GO_0005741; GO_0043332; GO_0005783; GO_0030133 1983 YFL007W BLM10 Proteasome activator Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 C IDA; IDA; IDA; IDA nucleus; proteasome storage granule; proteasome complex; proteasome core complex GO_0005634; GO_0034515; GO_0000502; GO_0005839 1984 YFL008W SMC1 Subunit of the multiprotein cohesin complex Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure C HDA; IDA nucleus; nuclear mitotic cohesin complex GO_0005634; GO_0034990 1985 YFL009W CDC4 F-box protein required for both the G1/S and G2/M phase transitions F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p C IDA; IDA; IDA; IDA nuclear matrix; SCF ubiquitin ligase complex; nucleus; nuclear SCF ubiquitin ligase complex GO_0016363; GO_0019005; GO_0005634; GO_0043224 1986 YFL010C WWM1 WW domain containing protein of unknown function WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 C HDA; HDA; IDA cytoplasm; mitochondrion; nucleus GO_0005737; GO_0005739; GO_0005634 1987 YFL010W-A AUA1 Protein required for the negative regulation by ammonia of Gap1p Protein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease 1988 YFL011W HXT10 Putative hexose transporter Putative hexose transporter; expressed at low levels and expression is repressed by glucose C HDA; HDA; HDA fungal-type vacuole; mitochondrion; cell periphery GO_0000324; GO_0005739; GO_0071944 1989 YFL012W YFL012W Putative protein of unknown function Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin 1990 YFL012W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene IES1/YFL013C 1991 YFL013C IES1 Subunit of the INO80 chromatin remodeling complex Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia C HDA; IPI; IDA nucleus; Ino80 complex; cytosol GO_0005634; GO_0031011; GO_0005829 1992 YFL013W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 1993 YFL014W HSP12 Plasma membrane protein involved in maintaining membrane organization Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension C HDA; HDA; HDA; IDA; IDA nucleus; cytoplasm; plasma membrane; cytosol; endosome GO_0005634; GO_0005737; GO_0005886; GO_0005829; GO_0005768 1994 YFL015C YFL015C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene 1995 YFL015W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YFL015C 1996 YFL016C MDJ1 Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones C HDA; IDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane; mitochondrial matrix GO_0005739; GO_0031314; GO_0005759 1997 YFL017C GNA1 Glucosamine-6-phosphate acetyltransferase Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 1998 YFL017W-A SMX2 Core Sm protein Sm G Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G C IDA; IDA; IDA; IDA U2-type prespliceosome; U4/U6 x U5 tri-snRNP complex; U5 snRNP; U1 snRNP GO_0071004; GO_0046540; GO_0005682; GO_0005685 1999 YFL018C LPD1 Dihydrolipoamide dehydrogenase Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication C HDA; IDA; IDA; IDA; IMP mitochondrion; mitochondrial nucleoid; mitochondrial oxoglutarate dehydrogenase complex; mitochondrial pyruvate dehydrogenase complex; glycine cleavage complex GO_0005739; GO_0042645; GO_0009353; GO_0005967; GO_0005960 2000 YFL019C YFL019C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YFL019C is not an essential gene 2001 YFL020C PAU5 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme C IDA; HDA; IDA integral component of membrane; fungal-type vacuole; cytoplasm GO_0016021; GO_0000324; GO_0005737 2002 YFL021C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF GAT1/YFL021W 2003 YFL021W GAT1 Transcriptional activator of nitrogen catabolite repression genes Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized C IPI; IDA nucleus; cytoplasm GO_0005634; GO_0005737 2004 YFL022C FRS2 Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar C IDA; HDA phenylalanine-tRNA ligase complex; cytoplasm GO_0009328; GO_0005737 2005 YFL023W BUD27 Unconventional prefoldin protein involved in translation initiation Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP C IDA; IDA cytoplasm; cytosolic ribosome GO_0005737; GO_0022626 2006 YFL024C EPL1 Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb; coding sequence contains length polymorphisms in different strains C IDA; IDA NuA4 histone acetyltransferase complex; Piccolo NuA4 histone acetyltransferase complex GO_0035267; GO_0032777 2007 YFL025C BST1 GPI inositol deacylase of the endoplasmic reticulum (ER) GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy C IDA endoplasmic reticulum GO_0005783 2008 YFL026W STE2 Receptor for alpha-factor pheromone Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells C IDA integral component of plasma membrane GO_0005887 2009 YFL027C GYP8 GTPase-activating protein for yeast Rab family members GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport C IDA intracellular GO_0005622 2010 YFL028C CAF16 Part of evolutionarily-conserved CCR4-NOT regulatory complex Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator C HDA; IDA cytoplasm; CCR4-NOT complex GO_0005737; GO_0030014 2011 YFL029C CAK1 Cyclin-dependent kinase-activating kinase Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases C IDA cytoplasm GO_0005737 2012 YFL030W AGX1 Alanine:glyoxylate aminotransferase (AGT) Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases C HDA; HDA mitochondrion; cytosol GO_0005739; GO_0005829 2013 YFL031C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps HAC1/YFL031W; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2014 YFL031W HAC1 Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog) Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress C IDA nucleus GO_0005634 2015 YFL032W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene 2016 YFL033C RIM15 Protein kinase involved in cell proliferation in response to nutrients Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2017 YFL034C-A RPL22B Ribosomal 60S subunit protein L22A Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2018 YFL034C-B MOB2 Activator of Cbk1p kinase Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p C IDA; IDA; IDA; IDA; IDA cellular bud; cellular bud neck; cellular bud tip; mating projection tip; nucleus GO_0005933; GO_0005935; GO_0005934; GO_0043332; GO_0005634 2019 YFL034W MIL1 Predicted lipase Putative integral membrane protein that interacts with Rpp0p; Rpp0p is a component of the ribosomal stalk 2020 YFL036W RPO41 Mitochondrial RNA polymerase Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation C HDA; HDA; IDA; IDA; IDA mitochondrion; cytoplasm; mitochondrial DNA-directed RNA polymerase complex; mitochondrial matrix; mitochondrial nucleoid GO_0005739; GO_0005737; GO_0034245; GO_0005759; GO_0042645 2021 YFL037W TUB2 Beta-tubulin Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria C IPI; IDA tubulin complex; spindle pole body GO_0045298; GO_0005816 2022 YFL038C YPT1 Rab family GTPase Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates unfolded protein response (UPR) by promoting the decay of HAC1 RNA C HDA; IDA; IDA; IDA; IDA; IDA; IDA mitochondrion; endoplasmic reticulum membrane; Golgi membrane; cytoplasmic vesicle; Golgi stack; pre-autophagosomal structure; cis-Golgi network GO_0005739; GO_0005789; GO_0000139; GO_0031410; GO_0005795; GO_0000407; GO_0005801 2023 YFL039C ACT1 Actin Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions C IDA; IDA; IDA; IDA; IPI; IDA; IDA actin cortical patch; actin filament; Swr1 complex; NuA4 histone acetyltransferase complex; Ino80 complex; actin filament bundle; cellular bud neck contractile ring GO_0030479; GO_0005884; GO_0000812; GO_0035267; GO_0031011; GO_0032432; GO_0000142 2024 YFL040W YFL040W Putative transporter Putative transporter; member of the sugar porter family; YFL040W is not an essential gene C HDA; HDA prospore membrane; fungal-type vacuole GO_0005628; GO_0000324 2025 YFL041W FET5 Multicopper oxidase Multicopper oxidase; integral membrane protein with similarity to Fet3p; may have a role in iron transport C HDA; HDA fungal-type vacuole membrane; endoplasmic reticulum GO_0000329; GO_0005783 2026 YFL041W-A YFL041W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 2027 YFL042C LAM5 Putative sterol transfer protein Putative protein of unknown function; not an essential gene; YFL042C has a paralog, YLR072W, that arose from the whole genome duplication C IDA; IDA; HDA ER-mitochondrion membrane contact site; nucleus-vacuole junction; endoplasmic reticulum GO_0044233; GO_0071561; GO_0005783 2028 YFL044C OTU1 Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2029 YFL045C SEC53 Phosphomannomutase Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen C IDA; IDA cytosol; cytoplasmic stress granule GO_0005829; GO_0010494 2030 YFL046W FMP32 Putative assembly factor for cytochrome c oxidase Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 2031 YFL047W RGD2 GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA; HDA cytoplasm; cellular bud neck; cellular bud tip GO_0005737; GO_0005935; GO_0005934 2032 YFL048C EMP47 Integral membrane component of ER-derived COPII-coated vesicles Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication C IDA; IDA; IDA; HDA integral component of endoplasmic reticulum membrane; integral component of Golgi membrane; ER to Golgi transport vesicle; fungal-type vacuole membrane GO_0030176; GO_0030173; GO_0030134; GO_0000329 2033 YFL049W SWP82 Member of the SWI/SNF chromatin remodeling complex Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions C IDA; HDA; IDA SWI/SNF complex; nucleus; cytosol GO_0016514; GO_0005634; GO_0005829 2034 YFL050C ALR2 Probable Mg(2+) transporter Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition C HDA; IMP cell periphery; plasma membrane GO_0071944; GO_0005886 2035 YFL051C YFL051C Putative protein of unknown function Putative protein of unknown function; YFL051C is not an essential gene C HDA cell periphery GO_0071944 2036 YFL052W ZNF1 Zinc cluster transcription factor that regulates respiratory growth Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity 2037 YFL053W DAK2 Dihydroxyacetone kinase Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation 2038 YFL054C AQY3 Putative channel-like protein Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol C HDA cell periphery GO_0071944 2039 YFL055W AGP3 Low-affinity amino acid permease Low-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition C HDA endoplasmic reticulum GO_0005783 2040 YFL056C AAD6 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family; expression induced in cells treated with the mycotoxin patulin 2041 YFL057C AAD16 Putative aryl alcohol dehydrogenase Putative aryl alcohol dehydrogenase; similar to Phanerochaete chrysosporium aryl alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 2042 YFL058W THI5 Protein involved in synthesis of the thiamine precursor HMP Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP 2043 YFL059W SNZ3 Member of a stationary phase-induced gene family Member of a stationary phase-induced gene family; expressed in the presence of galactose; transcription of SNZ3 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3 2044 YFL060C SNO3 Protein of unknown function Protein of unknown function; nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin 2045 YFL061W DDI2 Cyanamide hydratase that detoxifies cyanamide Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants 2046 YFL062W COS4 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C IDA; HDA; HDA; HDA; HDA endosome; fungal-type vacuole; nucleus; cytoplasm; fungal-type vacuole membrane GO_0005768; GO_0000324; GO_0005634; GO_0005737; GO_0000329 2047 YFL063W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2048 YFL064C YFL064C Putative protein of unknown function Putative protein of unknown function 2049 YFL065C YFL065C Putative protein of unknown function Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation 2050 YFL066C YFL066C Helicase-like protein encoded within the telomeric Y' element Helicase-like protein encoded within the telomeric Y' element; induced by treatment with 8-methoxypsoralen and UVA irradiation C HDA nucleus GO_0005634 2051 YFL067W YFL067W Protein of unknown function Protein of unknown function; down-regulated at low calcium levels 2052 YFL068W YFL068W Putative protein of unknown function Putative protein of unknown function C HDA cytosol GO_0005829 2053 YFR001W LOC1 Nucleolar protein involved in the asymmetric localization of ASH1 mRNA Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA; HDA; IDA preribosome, large subunit precursor; cytoplasm; nucleus; nucleolus GO_0030687; GO_0005737; GO_0005634; GO_0005730 2054 YFR002W NIC96 Linker nucleoporin component of the nuclear pore complex (NPC) Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) C IDA; IDA; IDA nuclear pore nuclear basket; nuclear pore; nuclear pore linkers GO_0044615; GO_0005643; GO_0044612 2055 YFR003C YPI1 Regulatory subunit of the type I protein phosphatase (PP1) Glc7p Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance C IDA nucleus GO_0005634 2056 YFR004W RPN11 Metalloprotease subunit of 19S regulatory particle Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA; IDA mitochondrion; nucleus; proteasome regulatory particle, lid subcomplex; cytosol; proteasome storage granule GO_0005739; GO_0005634; GO_0008541; GO_0005829; GO_0034515 2057 YFR005C SAD1 Conserved zinc-finger domain protein involved in pre-mRNA splicing Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle C IDA nucleus GO_0005634 2058 YFR006W YFR006W Putative X-Pro aminopeptidase Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene C HDA cytoplasm GO_0005737 2059 YFR007W YFH7 Putative kinase with similarity to the PRK/URK/PANK kinase subfamily Putative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase 2060 YFR008W FAR7 Protein involved in recovery from pheromone-induced cell cycle arrest Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress C HDA; IDA cytosol; endoplasmic reticulum GO_0005829; GO_0005783 2061 YFR009W GCN20 Positive regulator of the Gcn2p kinase activity Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA C IPI; IDA cytosol; cytosolic ribosome GO_0005829; GO_0022626 2062 YFR009W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YFR009W; identified by expression profiling and mass spectrometry 2063 YFR010W UBP6 Ubiquitin-specific protease Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance C IPI; IPI proteasome complex; proteasome regulatory particle GO_0000502; GO_0005838 2064 YFR010W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YFR011C; identified by expression profiling and mass spectrometry 2065 YFR011C MIC19 Component of the MICOS complex Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane C IDA; IDA; HDA; HDA mitochondrial crista junction; MICOS complex; cytoplasm; mitochondrion GO_0044284; GO_0061617; GO_0005737; GO_0005739 2066 YFR012W DCV1 Protein of unknown function Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication 2067 YFR012W-A YFR012W-A Putative protein of unknown function Putative protein of unknown function; identified by homology 2068 YFR013W IOC3 Subunit of the Isw1a complex Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication C IDA Isw1a complex GO_0036436 2069 YFR014C CMK1 Calmodulin-dependent protein kinase Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication C IPI cytoplasm GO_0005737 2070 YFR015C GSY1 Glycogen synthase Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2071 YFR016C YFR016C Protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene C HDA; HDA cytoplasm; cellular bud GO_0005737; GO_0005933 2072 YFR017C IGD1 Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 2073 YFR018C YFR018C Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 2074 YFR019W FAB1 1-phosphatidylinositol-3-phosphate 5-kinase 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis C IDA; IDA; IDA; HDA fungal-type vacuole membrane; endosome membrane; PAS complex; mitochondrion GO_0000329; GO_0010008; GO_0070772; GO_0005739 2075 YFR020W CSS2 Protein of unknown function, secreted when constitutively expressed Protein of unknown function; mRNA identified as translated by ribosome profiling data C HDA; HDA endoplasmic reticulum; extracellular region GO_0005783; GO_0005576 2076 YFR021W ATG18 Phosphoinositide binding protein Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood C IDA; IDA; HDA; IDA; HDA; IDA; IDA endosome; cytosol; fungal-type vacuole membrane; PAS complex; cell periphery; pre-autophagosomal structure; NA GO_0005768; GO_0005829; GO_0000329; GO_0070772; GO_0071944; GO_0000407; GO_0061908 2077 YFR022W ROG3 Alpha-arrestin involved in ubiquitin-dependent endocytosis Alpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; ROG3 has a paralog, ROD1, that arose from the whole genome duplication C HDA cytosol GO_0005829 2078 YFR023W PES4 Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication 2079 YFR024C-A LSB3 Protein containing a C-terminal SH3 domain Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication C HDA; HDA; HDA cellular bud neck; mitochondrion; cytoplasm GO_0005935; GO_0005739; GO_0005737 2080 YFR025C HIS2 Histidinolphosphatase Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control C HDA cytoplasm GO_0005737 2081 YFR026C ULI1 Protein of unknown function Putative protein of unknown function; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR) C HDA endoplasmic reticulum GO_0005783 2082 YFR027W ECO1 Acetyltransferase Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress C HDA; IPI; IDA; HDA cytoplasm; nuclear chromatin; nuclear replication fork; nucleus GO_0005737; GO_0000790; GO_0043596; GO_0005634 2083 YFR028C CDC14 Protein phosphatase required for mitotic exit Protein phosphatase required for mitotic exit; required for rDNA segregation; located in nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis C IDA; HDA; IDA; IDA; IDA nucleolus; nucleus; spindle pole body; cytoplasm; RENT complex GO_0005730; GO_0005634; GO_0005816; GO_0005737; GO_0030869 2084 YFR029W PTR3 Component of the SPS plasma membrane amino acid sensor system Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p C IDA extrinsic component of plasma membrane GO_0019897 2085 YFR030W MET10 Subunit alpha of assimilatory sulfite reductase Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide C IDA sulfite reductase complex (NADPH) GO_0009337 2086 YFR031C SMC2 Subunit of the condensin complex Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus C IDA; HDA nuclear condensin complex; mitochondrion GO_0000799; GO_0005739 2087 YFR031C-A RPL2A Ribosomal 60S subunit protein L2A Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication C IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 2088 YFR032C RRT5 Putative protein of unknown function Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation C HDA prospore membrane GO_0005628 2089 YFR032C-A RPL29 Ribosomal 60S subunit protein L29 Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog C HDA; IDA cytoplasm; cytosolic large ribosomal subunit GO_0005737; GO_0022625 2090 YFR032C-B MIN10 Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2091 YFR033C QCR6 Subunit 6 of the ubiquinol cytochrome-c reductase complex Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space C HDA; IDA; IDA; IDA mitochondrion; mitochondrial intermembrane space; mitochondrial respiratory chain complex III; nuclear periphery GO_0005739; GO_0005758; GO_0005750; GO_0034399 2092 YFR034C PHO4 Basic helix-loop-helix (bHLH) transcription factor of the myc-family Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2093 YFR034W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YFR035C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching 2094 YFR035C YFR035C Putative protein of unknown function Putative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein 2095 YFR036W CDC26 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA nucleus; cytosol; anaphase-promoting complex GO_0005634; GO_0005829; GO_0005680 2096 YFR036W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF RSC8/YFR037C 2097 YFR037C RSC8 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters C IDA; HDA RSC complex; nucleus GO_0016586; GO_0005634 2098 YFR038W IRC5 Putative ATPase containing the DEAD/H helicase-related sequence motif Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci C HDA nucleus GO_0005634 2099 YFR039C OSW7 Protein involved in outer spore wall assembly Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; deletion of both OSW7 and SHE10 results in reduced dityrosine fluorescence from the spore wall relative to other mutants C HDA endoplasmic reticulum GO_0005783 2100 YFR040W SAP155 Protein required for function of the Sit4p protein phosphatase Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication C IPI cytoplasm GO_0005737 2101 YFR041C ERJ5 Type I membrane protein with a J domain Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response C HDA endoplasmic reticulum GO_0005783 2102 YFR042W KEG1 Integral membrane protein of the ER Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 2103 YFR043C IRC6 Clathrin coat accessory factor Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci 2104 YFR044C DUG1 Cys-Gly metallo-di-peptidase Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) C HDA; IDA; HDA mitochondrion; cytoplasm; ribosome GO_0005739; GO_0005737; GO_0005840 2105 YFR045W YFR045W Putative mitochondrial transport protein Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white 2106 YFR046C CNN1 Kinetochore protein Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T C IDA; HDA; IDA Ndc80 complex; nucleus; kinetochore GO_0031262; GO_0005634; GO_0000776 2107 YFR047C BNA6 Quinolinate phosphoribosyl transferase Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2108 YFR048W RMD8 Cytosolic protein required for sporulation Cytosolic protein required for sporulation C HDA; HDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 2109 YFR049W YMR31 Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase Mitochondrial ribosomal protein of the small subunit; has similarity to human mitochondrial ribosomal protein MRP-S36 C HDA; IDA; IDA mitochondrion; mitochondrial oxoglutarate dehydrogenase complex; mitochondrial ribosome GO_0005739; GO_0009353; GO_0005761 2110 YFR050C PRE4 Beta 7 subunit of the 20S proteasome Beta 7 subunit of the 20S proteasome C IDA; IDA; HDA proteasome storage granule; proteasome core complex, beta-subunit complex; nucleus GO_0034515; GO_0019774; GO_0005634 2111 YFR051C RET2 Delta subunit of the coatomer complex (COPI) Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER C IMP COPI vesicle coat GO_0030126 2112 YFR052C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF HXK1/YFR053C 2113 YFR052W RPN12 Subunit of the 19S regulatory particle of the 26S proteasome lid Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress C IDA; IDA proteasome regulatory particle, lid subcomplex; proteasome storage granule GO_0008541; GO_0034515 2114 YFR053C HXK1 Hexokinase isoenzyme 1 Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication C HDA; HDA; IDA cytosol; mitochondrion; cytoplasm GO_0005829; GO_0005739; GO_0005737 2115 YFR054C YFR054C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2116 YFR055W IRC7 Beta-lyase involved in the production of thiols Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner 2117 YFR056C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W 2118 YFR057W YFR057W Putative protein of unknown function Putative protein of unknown function 2119 YGL001C ERG26 C-3 sterol dehydrogenase C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis C IDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 2120 YGL002W ERP6 Member of the p24 family involved in ER to Golgi transport Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; mitochondrion GO_0005783; GO_0005739 2121 YGL003C CDH1 Activator of anaphase-promoting complex/cyclosome (APC/C) Activator of anaphase-promoting complex/cyclosome (APC/C); cell-cycle regulated; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p C IPI; IDA; IDA anaphase-promoting complex; nucleus; cytoplasm GO_0005680; GO_0005634; GO_0005737 2122 YGL004C RPN14 Evolutionarily conserved 19S regulatory particle assembly-chaperone Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p C HDA; IDA; IDA cytoplasm; cytosol; nucleus GO_0005737; GO_0005829; GO_0005634 2123 YGL005C COG7 Component of the conserved oligomeric Golgi complex Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C HDA; IMP; HDA mitochondrion; Golgi transport complex; cytosol GO_0005739; GO_0017119; GO_0005829 2124 YGL006W PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a C IDA fungal-type vacuole membrane GO_0000329 2125 YGL006W-A YGL006W-A Putative protein of unknown function Putative protein of unknown function; identified by SAGE 2126 YGL007C-A YGL007C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding 2127 YGL007W BRP1 Putative protein of unknown function Dubious ORF located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1 2128 YGL008C PMA1 Plasma membrane P2-type H+-ATPase Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] C HDA; HDA; HDA; IDA cytosol; plasma membrane; mitochondrion; membrane raft GO_0005829; GO_0005886; GO_0005739; GO_0045121 2129 YGL009C LEU1 Isopropylmalate isomerase Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway C IDA; IDA cytoplasmic stress granule; cytosol GO_0010494; GO_0005829 2130 YGL010W MPO1 Protein involved in metabolism of phytosphingosine Protein involved in metabolism of phytosphingosine; not an essential gene C HDA endoplasmic reticulum GO_0005783 2131 YGL011C SCL1 Alpha 1 subunit of the 20S proteasome Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria C IDA; HDA proteasome core complex, alpha-subunit complex; mitochondrion GO_0019773; GO_0005739 2132 YGL012W ERG4 C-24(28) sterol reductase C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol C HDA endoplasmic reticulum GO_0005783 2133 YGL013C PDR1 Transcription factor that regulates the pleiotropic drug response Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 2134 YGL014C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry 2135 YGL014W PUF4 Member of the PUF protein family Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors C HDA cytoplasm GO_0005737 2136 YGL015C YGL015C Putative protein of unknown function Putative protein of unknown function; null mutants accumulate cargo in the Golgi C HDA prospore membrane GO_0005628 2137 YGL016W KAP122 Karyopherin beta Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance C IDA; IDA nuclear pore; cytoplasm GO_0005643; GO_0005737 2138 YGL017W ATE1 Arginyl-tRNA-protein transferase Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway C HDA cytoplasm GO_0005737 2139 YGL018C JAC1 Specialized J-protein that functions in Fe-S cluster biogenesis Specialized J-protein that functions in Fe-S cluster biogenesis; functions with Hsp70 in Fe-S cluster biogenesis in mitochondria; involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix C HDA mitochondrion GO_0005739 2140 YGL019W CKB1 Beta regulatory subunit of casein kinase 2 (CK2) Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases C IDA; IDA UTP-C complex; protein kinase CK2 complex GO_0034456; GO_0005956 2141 YGL020C GET1 Subunit of the GET complex Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance C HDA; IPI; HDA endoplasmic reticulum; GET complex; mitochondrion GO_0005783; GO_0043529; GO_0005739 2142 YGL021W ALK1 Protein kinase Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins 2143 YGL022W STT3 Subunit of the oligosaccharyltransferase complex of the ER lumen Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis C IPI; HDA oligosaccharyltransferase complex; endoplasmic reticulum GO_0008250; GO_0005783 2144 YGL023C PIB2 Protein of unknown function Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 C HDA mitochondrion GO_0005739 2145 YGL024W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C 2146 YGL025C PGD1 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor C IDA core mediator complex GO_0070847 2147 YGL026C TRP5 Tryptophan synthase Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2148 YGL027C CWH41 Processing alpha glucosidase I Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress C IDA; HDA endoplasmic reticulum membrane; fungal-type vacuole GO_0005789; GO_0000324 2149 YGL028C SCW11 Cell wall protein with similarity to glucanases Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 2150 YGL029W CGR1 Protein involved in nucleolar integrity and processing of pre-rRNA Protein involved in nucleolar integrity and processing of pre-rRNA; has a role in processing rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; relocalizes from nucleus to nucleolus upon DNA replication stress C HDA; HDA; IDA preribosome, large subunit precursor; nucleus; nucleolus GO_0030687; GO_0005634; GO_0005730 2151 YGL030W RPL30 Ribosomal 60S subunit protein L30 Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog C IDA; IDA cytoplasm; cytosolic large ribosomal subunit GO_0005737; GO_0022625 2152 YGL031C RPL24A Ribosomal 60S subunit protein L24A Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2153 YGL032C AGA2 Adhesion subunit of a-agglutinin of a-cells Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds C IDA; HDA; HDA fungal-type cell wall; fungal-type vacuole; endoplasmic reticulum GO_0009277; GO_0000324; GO_0005783 2154 YGL033W HOP2 Meiosis-specific protein that localizes to chromosomes Meiosis-specific protein that localizes to chromosomes;preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair; contains a second intron at the 3' end; heterodimer of Hop2p-Mnd1p stimulates the activity of Dmc1p C IDA condensed nuclear chromosome GO_0000794 2155 YGL034C YGL034C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2156 YGL035C MIG1 Transcription factor involved in glucose repression Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state C IDA; IDA; IDA nucleus; cytoplasm; nuclear envelope lumen GO_0005634; GO_0005737; GO_0005641 2157 YGL036W YGL036W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene C HDA cytoplasm GO_0005737 2158 YGL037C PNC1 Nicotinamidase that converts nicotinamide to nicotinic acid Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress C IDA; IDA; IDA cytoplasm; nucleus; peroxisome GO_0005737; GO_0005634; GO_0005777 2159 YGL038C OCH1 Mannosyltransferase of the cis-Golgi apparatus Mannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins C IDA Golgi cis cisterna GO_0000137 2160 YGL039W YGL039W Aldehyde reductase Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols C HDA cytoplasm GO_0005737 2161 YGL040C HEM2 Aminolevulinate dehydratase Aminolevulinate dehydratase; a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2162 YGL041C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2163 YGL041C-B YGL041C-B Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR C HDA endoplasmic reticulum GO_0005783 2164 YGL041W-A DPC13 Putative protein of unknown function Putative protein of unknown function; conserved in fungi; identified by expression profiling and mass spectrometry 2165 YGL042C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 2166 YGL043W DST1 General transcription elongation factor TFIIS General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress C IDA nucleus GO_0005634 2167 YGL044C RNA15 Component of the cleavage and polyadenylation factor I (CF I) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability C IPI mRNA cleavage factor complex GO_0005849 2168 YGL045W RIM8 Protein involved in proteolytic activation of Rim101p Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family C HDA; IDA cytosol; cytoplasmic side of plasma membrane GO_0005829; GO_0009898 2169 YGL047W ALG13 Catalytic component of UDP-GlcNAc transferase Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress C IDA; IPI; IDA; HDA; IDA; HDA cytoplasm; UDP-N-acetylglucosamine transferase complex; cytosol; nucleus; extrinsic component of endoplasmic reticulum membrane; endoplasmic reticulum GO_0005737; GO_0043541; GO_0005829; GO_0005634; GO_0042406; GO_0005783 2170 YGL048C RPT6 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA proteasome storage granule; nucleus; proteasome regulatory particle, base subcomplex GO_0034515; GO_0005634; GO_0008540 2171 YGL049C TIF4632 Translation initiation factor eIF4G Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication C IDA; IDA; IGI cytoplasmic stress granule; P-body; eukaryotic translation initiation factor 4F complex GO_0010494; GO_0000932; GO_0016281 2172 YGL050W TYW3 tRNA methyltransferase required for synthesis of wybutosine tRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions 2173 YGL051W MST27 Putative integral membrane protein, involved in vesicle formation Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles; MST27 has a paralog, MST28, that arose from a segmental duplication C IDA; HDA; IMP; IDA integral component of membrane; endoplasmic reticulum; plasma membrane; Golgi apparatus GO_0016021; GO_0005783; GO_0005886; GO_0005794 2174 YGL052W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MST27 2175 YGL053W PRM8 Pheromone-regulated protein Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication C IDA; IMP endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 2176 YGL054C ERV14 COPII-coated vesicle protein COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication C HDA; HDA; IDA; IDA cell periphery; endoplasmic reticulum; endoplasmic reticulum membrane; ER to Golgi transport vesicle GO_0071944; GO_0005783; GO_0005789; GO_0030134 2177 YGL055W OLE1 Delta(9) fatty acid desaturase Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 2178 YGL056C SDS23 Protein involved in cell separation during budding Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2179 YGL057C GEP7 Protein of unknown function Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 2180 YGL058W RAD6 Ubiquitin-conjugating enzyme (E2) Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, as well as endoplasmic reticulum-associated protein degradation (ERAD) with Ubr1p in the absence of canonical ER membrane ligases C IPI; IDA; IPI; IDA; IDA; IPI; IDA UBR1-RAD6 ubiquitin ligase complex; nuclear chromatin; proteasome complex; Rad6-Rad18 complex; nucleus; MUB1-RAD6-UBR2 ubiquitin ligase complex; cytoplasm GO_1990303; GO_0000790; GO_0000502; GO_0097505; GO_0005634; GO_1990304; GO_0005737 2181 YGL059W PKP2 Mitochondrial protein kinase Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress C HDA mitochondrion GO_0005739 2182 YGL060W YBP2 Central kinetochore associated protein Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication C IDA; HDA; IDA CENP-A containing nucleosome; cytoplasm; condensed nuclear chromosome kinetochore GO_0043505; GO_0005737; GO_0000778 2183 YGL061C DUO1 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 2184 YGL062W PYC1 Pyruvate carboxylase isoform Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication C IDA cytosol GO_0005829 2185 YGL063C-A Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2186 YGL063W PUS2 Mitochondrial tRNA:pseudouridine synthase Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 2187 YGL064C MRH4 Mitochondrial ATP-dependent RNA helicase of the DEAD-box family Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes C HDA; IDA; IDA mitochondrion; mitochondrial matrix; mitochondrial large ribosomal subunit GO_0005739; GO_0005759; GO_0005762 2188 YGL065C ALG2 Mannosyltransferase in the N-linked glycosylation pathway Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol C HDA endoplasmic reticulum GO_0005783 2189 YGL066W SGF73 Subunit of DUBm module of SAGA and SLIK SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; relocalizes to the cytosol in response to hypoxia; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay C IDA; IPI; IPI; IDA; IDA cytosol; SLIK (SAGA-like) complex; SAGA complex; DUBm complex; nucleus GO_0005829; GO_0046695; GO_0000124; GO_0071819; GO_0005634 2190 YGL067W NPY1 NADH diphosphatase (pyrophosphatase) NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member C HDA; HDA; IDA cytoplasm; cytosol; peroxisome GO_0005737; GO_0005829; GO_0005777 2191 YGL068W MNP1 Mitochondrial ribosomal protein of the large subunit Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth C HDA; IDA; IDA mitochondrion; mitochondrial nucleoid; mitochondrial large ribosomal subunit GO_0005739; GO_0042645; GO_0005762 2192 YGL069C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance 2193 YGL070C RPB9 RNA polymerase II subunit B12.6 RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription C IDA DNA-directed RNA polymerase II, core complex GO_0005665 2194 YGL071W AFT1 Transcription factor involved in iron utilization and homeostasis Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; HDA condensed nuclear chromosome kinetochore; nucleus; cytoplasm GO_0000778; GO_0005634; GO_0005737 2195 YGL072C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 2196 YGL073W HSF1 Trimeric heat shock transcription factor Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses, including hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell at the ribosome through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated C HDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 2197 YGL074C Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor 2198 YGL075C MPS2 Essential membrane protein localized at nuclear envelope and SPBs Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication C HDA; IDA; IDA endoplasmic reticulum; nuclear envelope; spindle pole body GO_0005783; GO_0005635; GO_0005816 2199 YGL076C RPL7A Ribosomal 60S subunit protein L7A Ribosomal 60S subunit protein L7A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication C HDA; IDA; IDA preribosome, large subunit precursor; cytoplasm; cytosolic large ribosomal subunit GO_0030687; GO_0005737; GO_0022625 2200 YGL077C HNM1 Plasma membrane transporter for choline, ethanolamine, and carnitine Plasma membrane transporter for choline, ethanolamine, and carnitine; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol C HDA; HDA endoplasmic reticulum; cell periphery GO_0005783; GO_0071944 2201 YGL078C DBP3 RNA-Dependent ATPase, member of DExD/H-box family RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype C IDA; IDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 2202 YGL079W KXD1 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome C IDA; HDA BLOC-1 complex; endosome GO_0031083; GO_0005768 2203 YGL080W MPC1 Highly conserved subunit of mitochondrial pyruvate carrier (MPC) Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Mpc1p and either Mpc2p or Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 2204 YGL081W YGL081W Putative protein of unknown function Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis 2205 YGL082W YGL082W Putative protein of unknown function Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication C HDA; HDA; HDA; HDA cell periphery; nucleus; cytoplasm; plasma membrane GO_0071944; GO_0005634; GO_0005737; GO_0005886 2206 YGL083W SCY1 Putative kinase Putative kinase; suppressor of GTPase mutant, similar to bovine rhodopsin kinase C HDA; HDA cytosol; clathrin-coated vesicle GO_0005829; GO_0030136 2207 YGL084C GUP1 Plasma membrane protein involved in remodeling GPI anchors Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; GUP1 has a paralog, GUP2, that arose from the whole genome duplication C HDA; IDA; IDA endoplasmic reticulum; plasma membrane; membrane GO_0005783; GO_0005886; GO_0016020 2208 YGL085W LCL3 Putative protein of unknown function Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS C HDA mitochondrion GO_0005739 2209 YGL086W MAD1 Coiled-coil protein involved in spindle-assembly checkpoint Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability C IDA; IDA; IDA nuclear pore; condensed nuclear chromosome kinetochore; nucleus GO_0005643; GO_0000778; GO_0005634 2210 YGL087C MMS2 Ubiquitin-conjugating enzyme variant Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress C IDA; IDA; IPI nucleus; cytoplasm; ubiquitin conjugating enzyme complex GO_0005634; GO_0005737; GO_0031371 2211 YGL088W YGL088W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing 2212 YGL089C MF(ALPHA)2 Mating pheromone alpha-factor, made by alpha cells Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed; MF(ALPHA)2 has a paralog, MF(ALPHA)1, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 2213 YGL090W LIF1 Component of the DNA ligase IV complex Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein C IDA; IDA; IPI nucleus; nuclear chromatin; DNA ligase IV complex GO_0005634; GO_0000790; GO_0032807 2214 YGL091C NBP35 Essential cytoplasmic iron-sulfur cluster binding protein Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases C IDA; IDA; IDA; IDA mitochondrion; cytoplasm; nucleus; Nbp35-Cfd1 ATPase complex GO_0005739; GO_0005737; GO_0005634; GO_1904564 2215 YGL092W NUP145 Essential protein with distinct roles in two nuclear pore subcomplexes Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 C IDA; IDA; IDA nuclear pore outer ring; nuclear pore central transport channel; nuclear pore GO_0031080; GO_0044613; GO_0005643 2216 YGL093W SPC105 Subunit of a kinetochore-microtubule binding complex Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation C IDA; HDA; IDA condensed nuclear chromosome kinetochore; mitochondrion; spindle pole body GO_0000778; GO_0005739; GO_0005816 2217 YGL094C PAN2 Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; complex acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes C IDA; HDA PAN complex; cytoplasm GO_0031251; GO_0005737 2218 YGL095C VPS45 Protein of the Sec1p/Munc-18 family Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment C IPI; IDA; IDA; IDA SNARE complex; extrinsic component of membrane; Golgi membrane; cytosol GO_0031201; GO_0019898; GO_0000139; GO_0005829 2219 YGL096W TOS8 Homeodomain-containing protein and putative transcription factor Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication C IDA nuclear chromatin GO_0000790 2220 YGL097W SRM1 Nucleotide exchange factor for Gsp1p Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p C IDA; IDA nucleus; nuclear chromatin GO_0005634; GO_0000790 2221 YGL098W USE1 Essential SNARE protein localized to the ER Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p C IDA; HDA; IDA integral component of cytoplasmic side of endoplasmic reticulum membrane; endoplasmic reticulum; SNARE complex GO_0071458; GO_0005783; GO_0031201 2222 YGL099W LSG1 Putative GTPase involved in 60S ribosomal subunit biogenesis Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm C HDA; IDA; HDA; IDA mating projection tip; cytosolic large ribosomal subunit; cytosol; cytoplasm GO_0043332; GO_0022625; GO_0005829; GO_0005737 2223 YGL100W SEH1 Subunit of the Nup84 nuclear pore and SEACAT subcomplexes Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 C IDA; IDA; IDA; IDA Seh1-associated complex; nuclear pore outer ring; extrinsic component of fungal-type vacuolar membrane; nuclear pore GO_0035859; GO_0031080; GO_0097042; GO_0005643 2224 YGL101W YGL101W Protein of unknown function Protein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2225 YGL102C Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein 2226 YGL103W RPL28 Ribosomal 60S subunit protein L28 Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance C IDA; IMP; HDA cytosolic large ribosomal subunit; nucleus; preribosome, large subunit precursor GO_0022625; GO_0005634; GO_0030687 2227 YGL104C VPS73 Mitochondrial protein Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family; VPS73 has a paralog, YBR241C, that arose from the whole genome duplication C HDA; HDA mitochondrion; fungal-type vacuole membrane GO_0005739; GO_0000329 2228 YGL105W ARC1 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p C IPI; IDA; IDA methionyl glutamyl tRNA synthetase complex; cytoplasm; cytoplasmic stress granule GO_0017102; GO_0005737; GO_0010494 2229 YGL106W MLC1 Essential light chain for Myo1p Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition C IDA; IPI; IDA; IDA cellular bud neck; myosin II complex; cellular bud tip; vesicle GO_0005935; GO_0016460; GO_0005934; GO_0031982 2230 YGL107C RMD9 Mitochondrial protein required for respiratory growth Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes; RMD9 has a paralog, YBR238C, that arose from the whole genome duplication C HDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0031314 2231 YGL108C YGL108C Protein of unknown function, predicted to be palmitoylated Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress C HDA cytosol GO_0005829 2232 YGL109W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C 2233 YGL110C CUE3 Protein of unknown function Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination C HDA cytoplasm GO_0005737 2234 YGL111W NSA1 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis C HDA; HDA; HDA nucleolus; preribosome, large subunit precursor; nucleus GO_0005730; GO_0030687; GO_0005634 2235 YGL112C TAF6 Subunit (60 kDa) of TFIID and SAGA complexes Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA SAGA complex; cytosol; SLIK (SAGA-like) complex; nucleus; transcription factor TFIID complex GO_0000124; GO_0005829; GO_0046695; GO_0005634; GO_0005669 2236 YGL113W SLD3 Protein involved in the initiation of DNA replication Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress C IDA; IPI; IDA; HDA chromosome, centromeric region; DNA replication preinitiation complex; nuclear chromatin; nucleus GO_0000775; GO_0031261; GO_0000790; GO_0005634 2237 YGL114W YGL114W Putative protein of unknown function Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters C HDA fungal-type vacuole membrane GO_0000329 2238 YGL115W SNF4 Activating gamma subunit of the AMP-activated Snf1p kinase complex Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; IDA; IDA; IDA cytosol; nucleotide-activated protein kinase complex; cytoplasm; nuclear envelope lumen; nucleus; plasma membrane GO_0005829; GO_0031588; GO_0005737; GO_0005641; GO_0005634; GO_0005886 2239 YGL116W CDC20 Activator of anaphase-promoting complex/cyclosome (APC/C) Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress C HDA; IPI; IPI; IDA; HDA nucleus; mitotic checkpoint complex; anaphase-promoting complex; nuclear periphery; cytoplasm GO_0005634; GO_0033597; GO_0005680; GO_0034399; GO_0005737 2240 YGL117W YGL117W Putative protein of unknown function Putative protein of unknown function 2241 YGL118C YGL118C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2242 YGL119W COQ8 ATPase required for ubiquinone biosynthesis and respiratory growth Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication C HDA; IDA; IDA mitochondrion; mitochondrial matrix; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0005759; GO_0031314 2243 YGL120C PRP43 RNA helicase in the DEAH-box family RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p C HDA; IMP; HDA 90S preribosome; post-mRNA release spliceosomal complex; mitochondrion GO_0030686; GO_0071014; GO_0005739 2244 YGL121C GPG1 Proposed gamma subunit of the heterotrimeric G protein Proposed gamma subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing 2245 YGL122C NAB2 Nuclear polyadenylated RNA-binding protein Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 2246 YGL123C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene RPS2/YGL123W 2247 YGL123W RPS2 Protein component of the small (40S) subunit Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 C IDA; IDA cytosolic small ribosomal subunit; small-subunit processome GO_0022627; GO_0032040 2248 YGL124C MON1 Subunit of a heterodimeric guanine nucleotide exchange factor (GEF) Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate C IDA; IDA; IPI; IDA cytosol; endosome; Mon1-Ccz1 complex; fungal-type vacuole membrane GO_0005829; GO_0005768; GO_0035658; GO_0000329 2249 YGL125W MET13 Major isozyme of methylenetetrahydrofolate reductase Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway C HDA mitochondrion GO_0005739 2250 YGL126W SCS3 Protein required for inositol prototrophy Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol C HDA endoplasmic reticulum GO_0005783 2251 YGL127C SOH1 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits C HDA; IDA nucleus; core mediator complex GO_0005634; GO_0070847 2252 YGL128C CWC23 Component of a complex containing Cef1p Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p C IDA U2-type spliceosomal complex GO_0005684 2253 YGL129C RSM23 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 2254 YGL130W CEG1 Guanylyltransferase involved in mRNA 5' capping Guanylyltransferase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5?-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide C IPI; IPI nucleus; mRNA cap methyltransferase complex GO_0005634; GO_0031533 2255 YGL131C SNT2 Subunit of Snt2C complex, RING finger ubiquitin ligase (E3) Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitylation, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA; HDA cytoplasm; Snt2C complex; nucleus GO_0005737; GO_0070211; GO_0005634 2256 YGL132W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C 2257 YGL133W ITC1 Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication C IPI; HDA CHRAC; nucleus GO_0008623; GO_0005634 2258 YGL134W PCL10 Pho85p cyclin Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication C IPI cyclin-dependent protein kinase holoenzyme complex GO_0000307 2259 YGL135W RPL1B Ribosomal 60S subunit protein L1B Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal C IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 2260 YGL136C MRM2 Mitochondrial 2' O-ribose methyltransferase Mitochondrial 2' O-ribose methyltransferase; required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ C IDA mitochondrion GO_0005739 2261 YGL137W SEC27 Essential beta'-coat protein of the COPI coatomer Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP C IGI COPI vesicle coat GO_0030126 2262 YGL138C YGL138C Putative protein of unknown function Putative protein of unknown function; has no significant sequence similarity to any known protein C HDA prospore membrane GO_0005628 2263 YGL139W FLC3 Putative FAD transporter, similar to Flc1p and Flc2p Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that arose from the whole genome duplication C HDA; IDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 2264 YGL140C YGL140C Putative protein of unknown function Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains C HDA endoplasmic reticulum GO_0005783 2265 YGL141W HUL5 Multiubiquitin chain assembly factor (E4) Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD C HDA; IDA; HDA cytoplasm; proteasome complex; nucleus GO_0005737; GO_0000502; GO_0005634 2266 YGL142C GPI10 Integral membrane protein involved in GPI anchor synthesis Integral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog 2267 YGL143C MRF1 Mitochondrial translation release factor Mitochondrial translation release factor; involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability C HDA mitochondrion GO_0005739 2268 YGL144C ROG1 Lipase with specificity for monoacylglycerol Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2269 YGL145W TIP20 Peripheral membrane protein required for COPI vesicle fusion to the ER Peripheral membrane protein required for COPI vesicle fusion to the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p C HDA; IDA; IDA cytosol; endoplasmic reticulum; Dsl1/NZR complex GO_0005829; GO_0005783; GO_0070939 2270 YGL146C RRT6 Putative protein of unknown function Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins 2271 YGL147C RPL9A Ribosomal 60S subunit protein L9A Ribosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication C IDA cytosolic large ribosomal subunit GO_0022625 2272 YGL148W ARO2 Bifunctional chorismate synthase and flavin reductase Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress C IDA cytoplasm GO_0005737 2273 YGL149W YGL149W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C 2274 YGL150C INO80 ATPase and nucleosome spacing factor ATPase and nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; promotes nucleosome shifts in the 3 prime direction; has a role in modulating stress gene transcription C IDA Ino80 complex GO_0031011 2275 YGL151W NUT1 Component of the RNA polymerase II mediator complex Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription C IDA; IDA; HDA nucleus; mediator complex; mitochondrion GO_0005634; GO_0016592; GO_0005739 2276 YGL152C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W 2277 YGL153W PEX14 Central component of the peroxisomal importomer complex Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p C IDA; IDA; IDA Pex17p-Pex14p docking complex; peroxisomal importomer complex; peroxisomal membrane GO_1990415; GO_1990429; GO_0005778 2278 YGL154C LYS5 Phosphopantetheinyl transferase involved in lysine biosynthesis Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine 2279 YGL155W CDC43 Beta subunit of geranylgeranyltransferase type I Beta subunit of geranylgeranyltransferase type I; catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis C IDA CAAX-protein geranylgeranyltransferase complex GO_0005953 2280 YGL156W AMS1 Vacuolar alpha mannosidase Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway C IDA fungal-type vacuole membrane GO_0000329 2281 YGL157W ARI1 NADPH-dependent aldehyde reductase NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2282 YGL158W RCK1 Protein kinase involved in oxidative stress response Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication 2283 YGL159W YGL159W Putative protein of unknown function Putative protein of unknown function; deletion mutant has no detectable phenotype 2284 YGL160W AIM14 NADPH oxidase localized to the perinuclear ER NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p C HDA; HDA; IDA endoplasmic reticulum; ribosome; perinuclear endoplasmic reticulum GO_0005783; GO_0005840; GO_0097038 2285 YGL161C YIP5 Protein that interacts with Rab GTPases Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport C IDA Golgi apparatus GO_0005794 2286 YGL162W SUT1 Zn(II)2Cys6 family transcription factor Transcription factor of the Zn(II)2Cys6 family; positively regulates genes involved in sterol uptake under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during non-starvation conditions; positively regulates mating with SUT2 by repressing expression of genes which act as mating inhibitors; relocalizes from nucleus to cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2287 YGL163C RAD54 DNA-dependent ATPase that stimulates strand exchange DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress C IMP; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2288 YGL164C YRB30 RanGTP-binding protein RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes C IMP; IDA nucleus; cytoplasm GO_0005634; GO_0005737 2289 YGL165C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W 2290 YGL166W CUP2 Copper-binding transcription factor Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication C IDA nucleus GO_0005634 2291 YGL167C PMR1 High affinity Ca2+/Mn2+ P-type ATPase High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen appears to be required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease C IDA Golgi membrane GO_0000139 2292 YGL168W HUR1 Protein of unknown function Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene 2293 YGL169W SUA5 Protein involved in threonylcarbamoyl adenosine biosynthesis Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 2294 YGL170C SPO74 Component of the meiotic outer plaque of the spindle pole body Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation C IMP; HDA meiotic spindle pole body; spindle pole body GO_0035974; GO_0005816 2295 YGL171W ROK1 RNA-dependent ATPase RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation C HDA; IDA 90S preribosome; nucleolus GO_0030686; GO_0005730 2296 YGL172W NUP49 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) C IDA; IDA; HDA nuclear pore; nuclear pore central transport channel; nuclear envelope GO_0005643; GO_0044613; GO_0005635 2297 YGL173C XRN1 Evolutionarily-conserved 5'-3' exonuclease Evolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; also enters the nucleus and positively regulates transcription initiation and elongation; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p C IMP; HDA; IDA; IDA; IDA P-body; cytoplasm; eisosome membrane domain/MCC; cytoplasmic stress granule; nucleus GO_0000932; GO_0005737; GO_0090512; GO_0010494; GO_0005634 2298 YGL174W BUD13 Subunit of the RES complex Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids C IDA; IDA RES complex; nucleus GO_0070274; GO_0005634 2299 YGL175C SAE2 Endonuclease required for telomere elongation Endonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently C HDA; IDA; HDA cytoplasm; Mre11 complex; nucleus GO_0005737; GO_0030870; GO_0005634 2300 YGL176C YGL176C Putative protein of unknown function Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype 2301 YGL177W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2302 YGL178W MPT5 mRNA-binding protein of the PUF family mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity C HDA cytoplasm GO_0005737 2303 YGL179C TOS3 Protein kinase Protein kinase; related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2304 YGL180W ATG1 Protein serine/threonine kinase Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation C HDA; IDA; IDA; IDA; IDA; IDA cytosol; ATG1/ULK1 kinase complex; NA; pre-autophagosomal structure; autophagosome membrane; NA GO_0005829; GO_1990316; GO_0120095; GO_0000407; GO_0000421; GO_0061908 2305 YGL181W GTS1 Protein involved in Arf3p regulation and in transcription regulation Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations C HDA; IDA; IDA cellular bud neck; nucleus; actin cortical patch GO_0005935; GO_0005634; GO_0030479 2306 YGL182C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MND1/YGL183C 2307 YGL183C MND1 Protein required for recombination and meiotic nuclear division Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks C HDA; IDA nucleus; condensed nuclear chromosome GO_0005634; GO_0000794 2308 YGL184C STR3 Peroxisomal cystathionine beta-lyase Peroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation C IDA; HDA; HDA peroxisome; cytoplasm; nucleus GO_0005777; GO_0005737; GO_0005634 2309 YGL185C YGL185C Putative protein with sequence similar to hydroxyacid dehydrogenases Putative protein with sequence similar to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2310 YGL186C TPN1 Plasma membrane pyridoxine (vitamin B6) transporter Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p C IDA; HDA plasma membrane; fungal-type vacuole GO_0005886; GO_0000324 2311 YGL187C COX4 Subunit IV of cytochrome c oxidase Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation C IDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 2312 YGL188C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2313 YGL188C-A YGL188C-A Putative protein of unknown function Putative protein of unknown function 2314 YGL189C RPS26A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26A has a paralog, RPS26B, that arose from the whole genome duplication 2315 YGL190C CDC55 Regulatory subunit B of protein phosphatase 2A (PP2A) Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55 C IDA; IDA; IDA; IDA; IDA; IDA; IDA cellular bud tip; protein phosphatase type 2A complex; fungal-type vacuole membrane; cytoplasm; mating projection tip; cellular bud neck; nucleus GO_0005934; GO_0000159; GO_0000329; GO_0005737; GO_0043332; GO_0005935; GO_0005634 2316 YGL191W COX13 Subunit VIa of cytochrome c oxidase Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes C IDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 2317 YGL192W IME4 mRNA N6-adenosine methyltransferase required for entry into meiosis mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70) C IDA MIS complex GO_0036396 2318 YGL193C YGL193C Haploid-specific gene repressed by a1-alpha2 Haploid-specific gene repressed by a1-alpha2; turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold 2319 YGL194C HOS2 Histone deacetylase and subunit of Set3 and Rpd3L complexes Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS C HDA; HDA; IDA; HDA; IDA cytoplasm; Rpd3L-Expanded complex; nuclear periphery; nucleus; Set3 complex GO_0005737; GO_0070210; GO_0034399; GO_0005634; GO_0034967 2320 YGL194C-A YGL194C-A Putative protein of unknown function Putative protein of unknown function; identified based on comparisons of the genome sequences of six Saccharomyces species C HDA endoplasmic reticulum GO_0005783 2321 YGL195W GCN1 Positive regulator of the Gcn2p kinase activity Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA C IDA; HDA; IDA cytosolic ribosome; mitochondrion; cytosol GO_0022626; GO_0005739; GO_0005829 2322 YGL196W DSD1 D-serine dehydratase (aka D-serine ammonia-lyase) D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate 2323 YGL197W MDS3 Putative component of the TOR regulatory pathway Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 2324 YGL198W YIP4 Protein that interacts with Rab GTPases Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport C IDA Golgi apparatus GO_0005794 2325 YGL199C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W 2326 YGL200C EMP24 Component of the p24 complex Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles C IDA; IDA ER to Golgi transport vesicle; endoplasmic reticulum GO_0030134; GO_0005783 2327 YGL201C MCM6 Protein involved in DNA replication Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA nucleus; MCM complex; replication fork protection complex; CMG complex; nuclear pre-replicative complex; MCM core complex; cytoplasm; DNA replication preinitiation complex GO_0005634; GO_0042555; GO_0031298; GO_0071162; GO_0005656; GO_0097373; GO_0005737; GO_0031261 2328 YGL202W ARO8 Aromatic aminotransferase I Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis C HDA cytoplasm GO_0005737 2329 YGL203C KEX1 Cell death protease essential for hypochlorite-induced apoptosis Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins C HDA; IDA fungal-type vacuole; trans-Golgi network GO_0000324; GO_0005802 2330 YGL204C YGL204C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data C HDA endoplasmic reticulum GO_0005783 2331 YGL205W POX1 Fatty-acyl coenzyme A oxidase Fatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix C IDA peroxisomal matrix GO_0005782 2332 YGL206C CHC1 Clathrin heavy chain Clathrin heavy chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion; the light chain (CLC1) is thought to regulate function 2333 YGL207W SPT16 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; SPT16 specifically required for diauxic shift-induced H2B deposition onto rDNA genes; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome C IPI; IPI; IDA; IDA; IDA transcription elongation factor complex; FACT complex; alpha DNA polymerase:primase complex; nuclear chromatin; replication fork protection complex GO_0008023; GO_0035101; GO_0005658; GO_0000790; GO_0031298 2334 YGL208W SIP2 One of three beta subunits of the Snf1 kinase complex One of three beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication C IDA; IDA; IDA nucleotide-activated protein kinase complex; cytoplasm; plasma membrane GO_0031588; GO_0005737; GO_0005886 2335 YGL209W MIG2 Zinc finger transcriptional repressor Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose C IDA; IDA nucleus; mitochondrion GO_0005634; GO_0005739 2336 YGL210W YPT32 Rab family GTPase involved in the exocytic pathway Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication C HDA; IDA; HDA; IDA cellular bud neck; endosome; mitochondrial outer membrane; Golgi apparatus GO_0005935; GO_0005768; GO_0005741; GO_0005794 2337 YGL211W NCS6 Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae C HDA; IDA mitochondrion; cytosol GO_0005739; GO_0005829 2338 YGL212W VAM7 Vacuolar SNARE protein Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress C IDA; IPI; IDA fungal-type vacuole membrane; SNARE complex; pre-autophagosomal structure GO_0000329; GO_0031201; GO_0000407 2339 YGL213C SKI8 Ski complex component and WD-repeat protein Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype C IPI; IDA Ski complex; nuclear chromosome GO_0055087; GO_0000228 2340 YGL214W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C 2341 YGL215W CLG1 Cyclin-like protein that interacts with Pho85p Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 C IPI cyclin-dependent protein kinase holoenzyme complex GO_0000307 2342 YGL216W KIP3 Kinesin-related antiparallel sliding motor protein Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone C IDA; IDA nuclear microtubule; cytoplasmic microtubule GO_0005880; GO_0005881 2343 YGL217C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W 2344 YGL218W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance 2345 YGL219C MDM34 Mitochondrial component of the ERMES complex Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase C HDA; IPI; HDA; HDA mitochondrial outer membrane; ERMES complex; cytoplasm; mitochondrion GO_0005741; GO_0032865; GO_0005737; GO_0005739 2346 YGL220W BOL2 Cytosolic protein involved in repression of iron regulon transcription Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel C IDA; HDA; HDA cytosol; nucleus; cytoplasm GO_0005829; GO_0005634; GO_0005737 2347 YGL221C NIF3 Protein of unknown function Protein of unknown function; similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2348 YGL222C EDC1 RNA-binding protein that activates mRNA decapping directly RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication C IPI; HDA; IDA Dcp1-Dcp2 complex; cytoplasm; nucleus GO_0098745; GO_0005737; GO_0005634 2349 YGL223C COG1 Essential component of the conserved oligomeric Golgi complex Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C IMP Golgi transport complex GO_0017119 2350 YGL224C SDT1 Pyrimidine nucleotidase Pyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication 2351 YGL225W VRG4 Golgi GDP-mannose transporter Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication C HDA; IDA mitochondrion; Golgi apparatus GO_0005739; GO_0005794 2352 YGL226C-A OST5 Zeta subunit of the oligosaccharyltransferase complex of the ER lumen Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins C IPI oligosaccharyltransferase complex GO_0008250 2353 YGL226W MTC3 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 C HDA mitochondrion GO_0005739 2354 YGL227W VID30 Central component of GID Complex, involved in FBPase degradation Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm C HDA; IDA; HDA nucleus; GID complex; cytoplasm GO_0005634; GO_0034657; GO_0005737 2355 YGL228W SHE10 Protein involved in outer spore wall assembly Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication and deletion of both SHE10 and OWS7 results in reduced dityrosine fluorescence from the spore wall relative to other mutants C HDA endoplasmic reticulum GO_0005783 2356 YGL229C SAP4 Protein required for function of the Sit4p protein phosphatase Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 2357 YGL230C YGL230C Putative protein of unknown function Putative protein of unknown function; non-essential gene C HDA; HDA endoplasmic reticulum; mitochondrion GO_0005783; GO_0005739 2358 YGL231C EMC4 Member of conserved ER transmembrane complex Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 C IDA; HDA ER membrane protein complex; endoplasmic reticulum GO_0072546; GO_0005783 2359 YGL232W TAN1 Putative tRNA acetyltransferase Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2360 YGL233W SEC15 Essential 113 kDa subunit of the exocyst complex Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase C IDA; IDA; IDA; IDA; HDA; HDA exocyst; cellular bud neck; incipient cellular bud site; cellular bud tip; mating projection tip; prospore membrane GO_0000145; GO_0005935; GO_0000131; GO_0005934; GO_0043332; GO_0005628 2361 YGL234W ADE5,7 Enzyme of the 'de novo' purine nucleotide biosynthetic pathway Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities C HDA cytoplasm GO_0005737 2362 YGL235W YGL235W Putative protein of unknown function Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 2363 YGL236C MTO1 Mitochondrial protein Mitochondrial protein; forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants C HDA mitochondrion GO_0005739 2364 YGL237C HAP2 Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding C IPI; HDA CCAAT-binding factor complex; nucleus GO_0016602; GO_0005634 2365 YGL238W CSE1 Nuclear envelope protein that acts as a recycling factor Nuclear envelope protein that mediates the nuclear export of Srp1p; Srp1p (importin alpha) is a homolog of metazoan CAS protein; required for accurate chromosome segregation C IDA nuclear envelope GO_0005635 2366 YGL239C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene CSE1 2367 YGL240W DOC1 Processivity factor Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain C IDA; HDA anaphase-promoting complex; mitochondrion GO_0005680; GO_0005739 2368 YGL241W KAP114 Karyopherin, responsible for nuclear import of specific proteins Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2369 YGL242C YGL242C Protein of unknown function Protein of unknown function; N-terminally propionylated in vivo; deletion mutant is viable 2370 YGL243W TAD1 tRNA-specific adenosine deaminase tRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala C HDA nucleus GO_0005634 2371 YGL244W RTF1 Subunit of RNAPII-associated chromatin remodeling Paf1 complex Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay C IPI; IDA; IDA Cdc73/Paf1 complex; nucleus; transcriptionally active chromatin GO_0016593; GO_0005634; GO_0035327 2372 YGL245W GUS1 Glutamyl-tRNA synthetase (GluRS) Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA cytoplasmic stress granule; methionyl glutamyl tRNA synthetase complex; cytoplasm; mitochondrion GO_0010494; GO_0017102; GO_0005737; GO_0005739 2373 YGL246C RAI1 Nuclear protein with decapping endonuclease activity Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 2374 YGL247W BRR6 Essential nuclear envelope integral membrane protein Essential nuclear envelope integral membrane protein; required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism C IDA; HDA; HDA nuclear envelope; cell periphery; endoplasmic reticulum GO_0005635; GO_0071944; GO_0005783 2375 YGL248W PDE1 Low-affinity cyclic AMP phosphodiesterase Low-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation 2376 YGL249W ZIP2 Meiosis-specific protein Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress C HDA; IDA mitochondrion; synaptonemal complex GO_0005739; GO_0000795 2377 YGL250W RMR1 Protein required for meiotic recombination and gene conversion Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2378 YGL251C HFM1 Meiosis specific DNA helicase Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity C IDA nucleus GO_0005634 2379 YGL252C RTG2 Sensor of mitochondrial dysfunction Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p C IDA; IDA; IDA SLIK (SAGA-like) complex; transcriptionally active chromatin; cytoplasm GO_0046695; GO_0035327; GO_0005737 2380 YGL253W HXK2 Hexokinase isoenzyme 2 Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication C IDA; IDA; IDA mitochondrion; cytosol; nucleus GO_0005739; GO_0005829; GO_0005634 2381 YGL254W FZF1 Transcription factor involved in sulfite metabolism Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress 2382 YGL255W ZRT1 High-affinity zinc transporter of the plasma membrane High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor C IMP; HDA; HDA integral component of plasma membrane; cell periphery; endoplasmic reticulum GO_0005887; GO_0071944; GO_0005783 2383 YGL256W ADH4 Alcohol dehydrogenase isoenzyme type IV Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency C HDA mitochondrion GO_0005739 2384 YGL257C MNT2 Mannosyltransferase Mannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans C HDA; HDA fungal-type vacuole membrane; cell periphery GO_0000329; GO_0071944 2385 YGL258W VEL1 Protein of unknown function Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication C HDA; IDA; HDA cell periphery; cytosol; endoplasmic reticulum GO_0071944; GO_0005829; GO_0005783 2386 YGL258W-A YGL258W-A Putative protein of unknown function Putative protein of unknown function 2387 YGL259W YPS5 Protein with similarity to GPI-anchored aspartic proteases Protein with similarity to GPI-anchored aspartic proteases; such proteases are Yap1p and Yap3p 2388 YGL260W YGL260W Putative protein of unknown function Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium 2389 YGL261C PAU11 Putative protein of unknown function Putative protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 2390 YGL262W YGL262W Putative protein of unknown function Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene 2391 YGL263W COS12 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA fungal-type vacuole GO_0000324 2392 YGR001C EFM5 S-adenosylmethionine-dependent lysine methyltransferase Putative protein of unknown function; similar to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae; expresses a circular RNA C HDA cytoplasm GO_0005737 2393 YGR002C SWC4 Component of the Swr1p complex that incorporates Htz1p into chromatin Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex C IPI; HDA; IDA; IPI NuA4 histone acetyltransferase complex; nucleus; Swr1 complex; histone acetyltransferase complex GO_0035267; GO_0005634; GO_0000812; GO_0000123 2394 YGR003W CUL3 Ubiquitin-protein ligase Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 C HDA; IMP; HDA nucleus; Cul3-RING ubiquitin ligase complex; cytoplasm GO_0005634; GO_0031463; GO_0005737 2395 YGR004W PEX31 Peroxisomal integral membrane protein Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication C HDA; IDA; HDA endoplasmic reticulum; integral component of peroxisomal membrane; cell periphery GO_0005783; GO_0005779; GO_0071944 2396 YGR005C TFG2 TFIIF (Transcription Factor II) middle subunit TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 C IDA transcription factor TFIIF complex GO_0005674 2397 YGR006W PRP18 Splicing factor and component of snRNP U5 Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p C IDA; IDA; IDA U4/U6 x U5 tri-snRNP complex; U5 snRNP; U2-type post-spliceosomal complex GO_0046540; GO_0005682; GO_0071021 2398 YGR007W ECT1 Ethanolamine-phosphate cytidylyltransferase Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2399 YGR008C STF2 Protein involved in resistance to desiccation stress Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 2400 YGR009C SEC9 t-SNARE protein required for secretory vesicle-plasma membrane fusion t-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functionally redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog C HDA; IDA; HDA; IDA cellular bud neck; extrinsic component of plasma membrane; cytosol; SNARE complex GO_0005935; GO_0019897; GO_0005829; GO_0031201 2401 YGR010W NMA2 Nicotinic acid mononucleotide adenylyltransferase Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication C IDA nucleus GO_0005634 2402 YGR011W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2403 YGR012W MCY1 Putative cysteine synthase Putative cysteine synthase; localized to the mitochondrial outer membrane C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 2404 YGR013W SNU71 Component of U1 snRNP required for mRNA splicing via spliceosome Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart C IDA; IDA U2-type prespliceosome; U1 snRNP GO_0071004; GO_0005685 2405 YGR014W MSB2 Mucin family member involved in various signaling pathways Mucin family member involved in various signaling pathways; functions as osmosensor in Sho1p-mediated HOG pathway with Msb2p; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate C IMP; IDA; IDA integral component of plasma membrane; extracellular region; site of polarized growth GO_0005887; GO_0005576; GO_0030427 2406 YGR015C EAT1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion C HDA mitochondrion GO_0005739 2407 YGR016W YGR016W Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 2408 YGR017W YGR017W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2409 YGR018C YGR018C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W 2410 YGR019W UGA1 Gamma-aminobutyrate (GABA) transaminase Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress C IDA cytosol GO_0005829 2411 YGR020C VMA7 Subunit F of the V1 peripheral membrane domain of V-ATPase Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits C HDA fungal-type vacuole membrane GO_0000329 2412 YGR021W DPC29 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 2413 YGR022C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W 2414 YGR023W MTL1 Putative plasma membrane sensor Putative plasma membrane sensor; involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication 2415 YGR024C THG1 tRNAHis guanylyltransferase tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2416 YGR025W YGR025W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2417 YGR026W YGR026W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery C HDA; HDA cell periphery; endoplasmic reticulum GO_0071944; GO_0005783 2418 YGR027C RPS25A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 2419 YGR027W-A YGR027W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 2420 YGR027W-B YGR027W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2421 YGR028W MSP1 Highly-conserved N-terminally anchored AAA-ATPase Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain C HDA; IDA; HDA mitochondrion; peroxisomal membrane; mitochondrial outer membrane GO_0005739; GO_0005778; GO_0005741 2422 YGR029W ERV1 Flavin-linked sulfhydryl oxidase of the mitochondrial IMS Flavin-linked sulfhydryl oxidase of the mitochondrial IMS; N-terminus is an intrinsically disordered domain that in the cytosol helps target Erv1p to mitochondria, and in the intermembrane space oxidizes Mia40p as part of a disulfide relay system that promotes intermembrane space retention of imported proteins; functional ortholog of human GFER (ALR) C IDA; HDA mitochondrial intermembrane space; mitochondrion GO_0005758; GO_0005739 2423 YGR030C POP6 Subunit of RNase MRP, nuclear RNase P and telomerase Subunit of both RNase MRP and nuclear RNase P; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA; IDA; IDA ribonuclease MRP complex; cytosol; nucleus; telomerase holoenzyme complex; nucleolar ribonuclease P complex GO_0000172; GO_0005829; GO_0005634; GO_0005697; GO_0005655 2424 YGR031C-A NAG1 Protein involved in yeast cell wall biogenesis Protein involved in yeast cell wall biogenesis; localizes to the cell periphery; production of Nag1p is dependent upon the presence of Slt2p and Rlm1p; gene is nested within and antisense to IMO32 C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 2425 YGR031W IMO32 Conserved mitochondrial protein of unknown function Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 C HDA mitochondrion GO_0005739 2426 YGR032W GSC2 Catalytic subunit of 1,3-beta-glucan synthase Catalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication C IDA; IDA; HDA; HDA prospore membrane; 1,3-beta-D-glucan synthase complex; cellular bud neck; cell periphery GO_0005628; GO_0000148; GO_0005935; GO_0071944 2427 YGR033C TIM21 Nonessential component of the TIM23 complex Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity C HDA; IDA mitochondrion; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0005744 2428 YGR034W RPL26B Ribosomal 60S subunit protein L26B Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2429 YGR035C YGR035C Putative protein of unknown function, potential Cdc28p substrate Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication 2430 YGR035W-A YGR035W-A Putative protein of unknown function Putative protein of unknown function 2431 YGR036C CAX4 Dolichyl pyrophosphate (Dol-P-P) phosphatase Dolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminally oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 2432 YGR037C ACB1 Acyl-CoA-binding protein Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress C HDA; HDA; IMP; HDA cytosol; cytoplasm; extracellular region; nucleus GO_0005829; GO_0005737; GO_0005576; GO_0005634 2433 YGR038C-A YGR038C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 2434 YGR038C-B YGR038C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2435 YGR038W ORM1 Protein that mediates sphingolipid homeostasis Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; SPOTS complex GO_0005783; GO_0035339 2436 YGR039W YGR039W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 2437 YGR040W KSS1 Mitogen-activated protein kinase (MAPK) Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains C IDA; IDA nucleus; cellular bud neck GO_0005634; GO_0005935 2438 YGR041W BUD9 Protein involved in bud-site selection Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication C IDA; IMP cellular bud neck; plasma membrane GO_0005935; GO_0005886 2439 YGR042W MTE1 Protein of unknown function Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress C HDA; HDA; IDA; IDA nucleus; cytoplasm; site of double-strand break; chromosome, telomeric region GO_0005634; GO_0005737; GO_0035861; GO_0000781 2440 YGR043C NQM1 Transaldolase of unknown function Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication C HDA nucleus GO_0005634 2441 YGR044C RME1 Zinc finger protein involved in control of meiosis Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2442 YGR045C YGR045C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2443 YGR046W TAM41 Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase) Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase); required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition C HDA; IDA; IDA mitochondrion; mitochondrial matrix; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0005759; GO_0031314 2444 YGR047C TFC4 Subunit of RNA polymerase III transcription initiation factor complex Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 C HDA; IDA; HDA nucleus; transcription factor TFIIIC complex; cytoplasm GO_0005634; GO_0000127; GO_0005737 2445 YGR048W UFD1 Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) C HDA; IDA; IDA; IDA; IDA; HDA cytosol; Hrd1p ubiquitin ligase ERAD-L complex; Doa10p ubiquitin ligase complex; RQC complex; VCP-NPL4-UFD1 AAA ATPase complex; nucleus GO_0005829; GO_0000839; GO_0000837; GO_1990112; GO_0034098; GO_0005634 2446 YGR049W SCM4 Mitochondrial outer membrane protein of unknown function Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 2447 YGR050C YGR050C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data 2448 YGR051C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene 2449 YGR052W FMP48 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation C HDA mitochondrion GO_0005739 2450 YGR053C MCO32 Putative protein of unknown function Putative protein of unknown function 2451 YGR054W YGR054W Eukaryotic initiation factor eIF2A Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A C HDA; IDA; IDA ribosome; cytosolic small ribosomal subunit; cytosolic ribosome GO_0005840; GO_0022627; GO_0022626 2452 YGR055W MUP1 High affinity methionine permease High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake C IDA; HDA; HDA plasma membrane; fungal-type vacuole; cell periphery GO_0005886; GO_0000324; GO_0071944 2453 YGR056W RSC1 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication C IDA RSC complex GO_0016586 2454 YGR057C LST7 Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface C HDA; IDA; IDA; IDA cytosol; vacuolar membrane; Lst4-Lst7 complex; cytoplasm GO_0005829; GO_0005774; GO_1990877; GO_0005737 2455 YGR058W PEF1 Penta-EF-hand protein Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly C IDA; IDA; HDA; IDA; HDA cytosol; cellular bud neck; nucleus; site of polarized growth; cytoplasm GO_0005829; GO_0005935; GO_0005634; GO_0030427; GO_0005737 2456 YGR059W SPR3 Sporulation-specific homolog of the CDC3/10/11/12 family of genes Sporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes C HDA; IDA; IDA; IDA cytosol; ascospore wall; septin complex; prospore membrane GO_0005829; GO_0005619; GO_0031105; GO_0005628 2457 YGR060W ERG25 C-4 methyl sterol oxidase C-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol C IDA; IDA endoplasmic reticulum membrane; plasma membrane GO_0005789; GO_0005886 2458 YGR061C ADE6 Formylglycinamidine-ribonucleotide (FGAM)-synthetase Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway C HDA cytoplasm GO_0005737 2459 YGR062C COX18 Protein required for membrane insertion of C-terminus of Cox2p Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC C IDA integral component of mitochondrial inner membrane GO_0031305 2460 YGR063C SPT4 Spt4p/5p (DSIF) transcription elongation factor complex subunit Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions C IPI; IDA; IDA; IDA; IDA DSIF complex; nuclear telomeric heterochromatin; kinetochore; rDNA heterochromatin; mating-type region heterochromatin GO_0032044; GO_0005724; GO_0000776; GO_0033553; GO_0031934 2461 YGR064W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C 2462 YGR065C VHT1 High-affinity plasma membrane H+-biotin (vitamin H) symporter High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 2463 YGR066C YGR066C Putative protein of unknown function Putative protein of unknown function 2464 YGR067C YGR067C Putative protein of unknown function Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p 2465 YGR068C ART5 Protein proposed to regulate endocytosis of plasma membrane proteins Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane C HDA cytosol GO_0005829 2466 YGR068W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with the uncharacterized ORF YGR068C 2467 YGR069W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2468 YGR070W ROM1 Guanine nucleotide exchange factor (GEF) for Rho1p GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication C IDA intracellular GO_0005622 2469 YGR071C ENV11 Protein proposed to be involved in vacuolar functions Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication C HDA nucleus GO_0005634 2470 YGR072W UPF3 Component of the nonsense-mediated mRNA decay (NMD) pathway Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance C IDA; IDA; IDA; IDA nucleolus; nucleus; cytoplasm; polysome GO_0005730; GO_0005634; GO_0005737; GO_0005844 2471 YGR073C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps essential SMD1 gene encoding a U6 snRNP protein 2472 YGR074W SMD1 Core Sm protein Sm D1 Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA; IDA; IDA; IPI nucleus; U2-type prespliceosome; cytosol; U1 snRNP; U4/U6 x U5 tri-snRNP complex; U5 snRNP; commitment complex GO_0005634; GO_0071004; GO_0005829; GO_0005685; GO_0046540; GO_0005682; GO_0000243 2473 YGR075C PRP38 Unique component of the U4/U6.U5 tri-snRNP particle Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly C IDA U4/U6 x U5 tri-snRNP complex GO_0046540 2474 YGR076C MRPL25 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 2475 YGR077C PEX8 Intraperoxisomal organizer of the peroxisomal import machinery Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p C IDA; IDA peroxisomal importomer complex; extrinsic component of intraperoxisomal membrane GO_1990429; GO_0005780 2476 YGR078C PAC10 Part of the heteromeric co-chaperone GimC/prefoldin complex Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding C IDA; IDA; IPI cytoplasm; polysome; prefoldin complex GO_0005737; GO_0005844; GO_0016272 2477 YGR079W YGR079W Putative protein of unknown function Putative protein of unknown function; YGR079W is not an essential gene 2478 YGR080W TWF1 Twinfilin Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly C IDA; HDA actin filament; mating projection tip GO_0005884; GO_0043332 2479 YGR081C SLX9 Protein required for pre-rRNA processing Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant C IDA; IDA; HDA; HDA preribosome, small subunit precursor; 90S preribosome; nucleolus; nucleus GO_0030688; GO_0030686; GO_0005730; GO_0005634 2480 YGR082W TOM20 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins C IDA; IDA; HDA; HDA mitochondrial outer membrane translocase complex; integral component of mitochondrial outer membrane; mitochondrion; mitochondrial outer membrane GO_0005742; GO_0031307; GO_0005739; GO_0005741 2481 YGR083C GCD2 Delta subunit of the translation initiation factor eIF2B Delta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression C IPI; HDA; IDA eukaryotic translation initiation factor 2B complex; cytosol; guanyl-nucleotide exchange factor complex GO_0005851; GO_0005829; GO_0032045 2482 YGR084C MRP13 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 2483 YGR085C RPL11B Ribosomal 60S subunit protein L11B Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2484 YGR086C PIL1 Eisosome core component Eisosome core component; eisosomes are large immobile cell cortex structures associated with endocytosis; detected in phosphorylated state in mitochondria; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; member of BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress C HDA; IDA; HDA; HDA; HDA; HDA plasma membrane; eisosome; cell periphery; cytoplasm; mitochondrion; mitochondrial outer membrane GO_0005886; GO_0032126; GO_0071944; GO_0005737; GO_0005739; GO_0005741 2485 YGR087C PDC6 Minor isoform of pyruvate decarboxylase Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation C HDA cytoplasm GO_0005737 2486 YGR088W CTT1 Cytosolic catalase T Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide C HDA cytoplasm GO_0005737 2487 YGR089W NNF2 Protein that exhibits physical and genetic interactions with Rpb8p Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation C HDA endoplasmic reticulum GO_0005783 2488 YGR090W UTP22 Component of the small-subunit processome Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals C IDA; IDA; HDA; IDA; IDA; HDA CURI complex; nucleolus; 90S preribosome; UTP-C complex; small-subunit processome; nucleus GO_0032545; GO_0005730; GO_0030686; GO_0034456; GO_0032040; GO_0005634 2489 YGR091W PRP31 Splicing factor Splicing factor; component of the U4/U6-U5 snRNP complex C IDA; HDA U4/U6 x U5 tri-snRNP complex; mitochondrion GO_0046540; GO_0005739 2490 YGR092W DBF2 Ser/Thr kinase involved in transcription and stress response Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication C IDA; IDA cellular bud neck; spindle pole body GO_0005935; GO_0005816 2491 YGR093W DRN1 Splicing factor that modulates turnover of branched RNAs by Dbr1p Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia C HDA; IDA nucleus; cytosol GO_0005634; GO_0005829 2492 YGR094W VAS1 Mitochondrial and cytoplasmic valyl-tRNA synthetase Mitochondrial and cytoplasmic valyl-tRNA synthetase C HDA; IMP mitochondrion; cytoplasm GO_0005739; GO_0005737 2493 YGR095C RRP46 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) C IDA; IDA nuclear exosome (RNase complex); cytoplasmic exosome (RNase complex) GO_0000176; GO_0000177 2494 YGR096W TPC1 Mitochondrial membrane transporter Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family C IDA integral component of mitochondrial inner membrane GO_0031305 2495 YGR097W ASK10 Regulator of the Fps1p glycerol channel Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication C IDA; HDA DNA-directed RNA polymerase II, holoenzyme; cytoplasm GO_0016591; GO_0005737 2496 YGR098C ESP1 Separase, a caspase-like cysteine protease Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; IDA; HDA spindle; cytoplasm; nucleus; mitochondrion GO_0005819; GO_0005737; GO_0005634; GO_0005739 2497 YGR099W TEL2 Subunit of the ASTRA complex, involved in chromatin remodeling Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 C IDA; HDA nuclear chromosome, telomeric region; ASTRA complex GO_0000784; GO_0070209 2498 YGR100W MDR1 Cytoplasmic GTPase-activating protein Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function C IDA cytoplasm GO_0005737 2499 YGR101W PCP1 Mitochondrial serine protease Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases C IDA mitochondrial inner membrane GO_0005743 2500 YGR102C GTF1 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria C IDA; IDA mitochondrion; glutamyl-tRNA(Gln) amidotransferase complex GO_0005739; GO_0030956 2501 YGR103W NOP7 Component of several different pre-ribosomal particles Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation C IDA; IPI; IDA; IDA preribosome, large subunit precursor; PeBoW complex; nucleus; nucleolus GO_0030687; GO_0070545; GO_0005634; GO_0005730 2502 YGR104C SRB5 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance C IDA core mediator complex GO_0070847 2503 YGR105W VMA21 Integral membrane protein required for V-ATPase function Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) C IDA; HDA endoplasmic reticulum membrane; endoplasmic reticulum GO_0005789; GO_0005783 2504 YGR106C VOA1 ER protein that functions in assembly of the V0 sector of V-ATPase ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval C HDA; IDA fungal-type vacuole membrane; endoplasmic reticulum membrane GO_0000329; GO_0005789 2505 YGR107W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2506 YGR108W CLB1 B-type cyclin involved in cell cycle progression B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication C IMP; HDA; IDA cyclin-dependent protein kinase holoenzyme complex; nucleus; cytoplasm GO_0000307; GO_0005634; GO_0005737 2507 YGR109C CLB6 B-type cyclin involved in DNA replication during S phase B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication 2508 YGR109W-A YGR109W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag C IMP; IDA P-body; retrotransposon nucleocapsid GO_0000932; GO_0000943 2509 YGR109W-B YGR109W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2510 YGR110W CLD1 Mitochondrial cardiolipin-specific phospholipase Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 C IDA; IDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 2511 YGR111W YGR111W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2512 YGR112W SHY1 Mitochondrial inner membrane protein required for complex IV assembly Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase C IDA; IDA; HDA integral component of membrane; mitochondrial inner membrane; mitochondrion GO_0016021; GO_0005743; GO_0005739 2513 YGR113W DAM1 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments C IDA DASH complex GO_0042729 2514 YGR114C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 2515 YGR115C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C 2516 YGR116W SPT6 Nucleosome remodeling protein Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p C HDA; IDA; IDA mitochondrion; transcriptionally active chromatin; nucleus GO_0005739; GO_0035327; GO_0005634 2517 YGR117C YGR117C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2518 YGR118W RPS23A Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal C IDA cytosolic small ribosomal subunit GO_0022627 2519 YGR119C NUP57 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) C IDA; IDA nuclear pore central transport channel; nuclear pore GO_0044613; GO_0005643 2520 YGR120C COG2 Essential component of the conserved oligomeric Golgi complex Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p C HDA; IMP; IDA cytosol; Golgi transport complex; extrinsic component of membrane GO_0005829; GO_0017119; GO_0019898 2521 YGR121C MEP1 Ammonium permease Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation; MEP1 has a paralog, MEP3, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 2522 YGR121W-A YGR121W-A Putative protein of unknown function Putative protein of unknown function 2523 YGR122C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; similar to YLR334C and YOL106W 2524 YGR122W YGR122W Protein that may be involved in pH regulation Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress C HDA; HDA; HDA plasma membrane; nucleus; cytoplasm GO_0005886; GO_0005634; GO_0005737 2525 YGR123C PPT1 Protein serine/threonine phosphatase Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 2526 YGR124W ASN2 Asparagine synthetase Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 2527 YGR125W YGR125W Putative protein of unknown function Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole C HDA fungal-type vacuole GO_0000324 2528 YGR126W YGR126W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2529 YGR127W YGR127W Putative protein of unknown function Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response 2530 YGR128C UTP8 Nucleolar protein required for export of tRNAs from the nucleus Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C HDA; IDA; IDA; IDA; IDA 90S preribosome; rDNA heterochromatin; small-subunit processome; nucleolus; t-UTP complex GO_0030686; GO_0033553; GO_0032040; GO_0005730; GO_0034455 2531 YGR129W SYF2 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest C IDA; IDA; IDA; IDA; IDA; IDA; IDA U2-type post-mRNA release spliceosomal complex; U2-type catalytic step 1 spliceosome; cytosol; U2-type catalytic step 2 spliceosome; U2-type prespliceosome; Prp19 complex; nucleus GO_0071008; GO_0071006; GO_0005829; GO_0071007; GO_0071004; GO_0000974; GO_0005634 2532 YGR130C YGR130C Component of the eisosome with unknown function Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) C HDA; IDA; IDA cytoplasm; eisosome; membrane raft GO_0005737; GO_0032126; GO_0045121 2533 YGR131W FHN1 Protein of unknown function Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication C HDA; IDA cell periphery; membrane raft GO_0071944; GO_0045121 2534 YGR132C PHB1 Subunit of the prohibitin complex (Phb1p-Phb2p) Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) C IDA; IDA; HDA mitochondrial inner membrane; peroxisomal importomer complex; mitochondrion GO_0005743; GO_1990429; GO_0005739 2535 YGR133W PEX4 Peroxisomal ubiquitin conjugating enzyme Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis C IDA peroxisome GO_0005777 2536 YGR134W CAF130 Subunit of the CCR4-NOT transcriptional regulatory complex Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation C IPI; HDA CCR4-NOT core complex; cytosol GO_0030015; GO_0005829 2537 YGR135W PRE9 Alpha 3 subunit of the 20S proteasome Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform C IDA; IDA proteasome core complex, alpha-subunit complex; proteasome storage granule GO_0019773; GO_0034515 2538 YGR136W LSB1 Negative regulator of actin nucleation-promoting factor activity Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication C HDA; IDA; HDA cytoplasm; actin cortical patch; nucleus GO_0005737; GO_0030479; GO_0005634 2539 YGR137W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2540 YGR138C TPO2 Polyamine transporter of the major facilitator superfamily Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication C IDA; IMP; HDA plasma membrane; fungal-type vacuole membrane; cell periphery GO_0005886; GO_0000329; GO_0071944 2541 YGR139W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2542 YGR140W CBF2 Essential kinetochore protein Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress C IDA; HDA; IDA; IDA; IDA CBF3 complex; nucleus; spindle; condensed nuclear chromosome, centromeric region; spindle midzone GO_0031518; GO_0005634; GO_0005819; GO_0000780; GO_0051233 2543 YGR141W VPS62 Vacuolar protein sorting (VPS) protein Vacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; VPS62 has a paralog, TDA6, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole membrane; endoplasmic reticulum GO_0000329; GO_0005783 2544 YGR142W BTN2 v-SNARE binding protein v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; BTN2 has a paralog, CUR1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA nucleus; nuclear periphery; perinuclear region of cytoplasm; cytosol; late endosome GO_0005634; GO_0034399; GO_0048471; GO_0005829; GO_0005770 2545 YGR143W SKN1 Protein involved in sphingolipid biosynthesis Protein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication C IDA integral component of membrane GO_0016021 2546 YGR144W THI4 Thiazole synthase Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents C IDA cytosol GO_0005829 2547 YGR145W ENP2 Component of the SSU Component of the SSU; required for pre-18S rRNA processing, biogenesis of the small ribosomal subunit; interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD repeats, and has homology to Spb1p C HDA; IMP; IDA 90S preribosome; small-subunit processome; nucleolus GO_0030686; GO_0032040; GO_0005730 2548 YGR146C ECL1 Protein of unknown function Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation 2549 YGR146C-A YGR146C-A Putative protein of unknown function Putative protein of unknown function 2550 YGR147C NAT2 Protein of unknown function Protein of unknown function; has an apparent role in acetylation of N-terminal methionine residues C HDA mitochondrion GO_0005739 2551 YGR148C RPL24B Ribosomal 60S subunit protein L24B Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2552 YGR149W GPC1 Glycerophosphocholine acyltransferase (GPCAT) Putative protein of unknown function; predicted to be an integal membrane protein C HDA; HDA cell periphery; endoplasmic reticulum GO_0071944; GO_0005783 2553 YGR150C CCM1 Mitochondrial 15S rRNA-binding protein Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport C HDA mitochondrion GO_0005739 2554 YGR151C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress 2555 YGR152C RSR1 GTP-binding protein of the Ras superfamily GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases C IDA; IDA; HDA; HDA cellular bud neck; plasma membrane; cell periphery; endoplasmic reticulum GO_0005935; GO_0005886; GO_0071944; GO_0005783 2556 YGR153W YGR153W Putative protein of unknown function Putative protein of unknown function 2557 YGR154C GTO1 Omega-class glutathione transferase Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization C IDA peroxisome GO_0005777 2558 YGR155W CYS4 Cystathionine beta-synthase Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria C IDA; IDA; HDA cytoplasmic stress granule; cytoplasm; mitochondrion GO_0010494; GO_0005737; GO_0005739 2559 YGR156W PTI1 Essential component of CPF (cleavage and polyadenylation factor) Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64 C HDA; IDA; IDA nucleus; cytosol; mRNA cleavage and polyadenylation specificity factor complex GO_0005634; GO_0005829; GO_0005847 2560 YGR157W CHO2 Phosphatidylethanolamine methyltransferase (PEMT) Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis C HDA; HDA endoplasmic reticulum; cell periphery GO_0005783; GO_0071944 2561 YGR158C MTR3 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) C IDA; IDA; IDA cytoplasmic exosome (RNase complex); nuclear exosome (RNase complex); nucleolus GO_0000177; GO_0000176; GO_0005730 2562 YGR159C NSR1 Nucleolar protein that binds nuclear localization sequences Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis C HDA; IDA; HDA; IDA nucleolus; nucleus; mitochondrion; nuclear envelope GO_0005730; GO_0005634; GO_0005739; GO_0005635 2563 YGR160W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2564 YGR161C RTS3 Putative component of the protein phosphatase type 2A complex Putative component of the protein phosphatase type 2A complex C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2565 YGR161C-C YGR161C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 2566 YGR161C-D YGR161C-D Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2567 YGR161W-A YGR161W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 2568 YGR161W-B YGR161W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2569 YGR161W-C YGR161W-C Putative protein of unknown function Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species 2570 YGR162W TIF4631 Translation initiation factor eIF4G Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication C IDA; IMP; HDA; IDA; IDA cytoplasm; eukaryotic translation initiation factor 4F complex; mitochondrion; cytoplasmic stress granule; P-body GO_0005737; GO_0016281; GO_0005739; GO_0010494; GO_0000932 2571 YGR163W GTR2 Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex Putative GTP binding protein; negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD C IDA; IDA; IPI; IDA; IDA; IDA; IDA late endosome membrane; EGO complex; Gtr1-Gtr2 GTPase complex; fungal-type vacuole membrane; nuclear chromatin; nucleus; cytoplasm GO_0031902; GO_0034448; GO_1990131; GO_0000329; GO_0000790; GO_0005634; GO_0005737 2572 YGR164W YGR164W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2573 YGR165W MRPS35 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 2574 YGR166W TRS65 Component of transport protein particle (TRAPP) complex II Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response C IDA; IDA TRAPPII protein complex; trans-Golgi network GO_1990071; GO_0005802 2575 YGR167W CLC1 Clathrin light chain Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion C IDA trans-Golgi network GO_0005802 2576 YGR168C PEX35 Putative protein of unknown function Putative protein of unknown function; YGR168C is not an essential gene C HDA peroxisome GO_0005777 2577 YGR169C PUS6 tRNA:pseudouridine synthase tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability C IDA; IDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2578 YGR169C-A LSO2 Protein with a potential role in response to iron deprivation Putative protein of unknown function; LSO2 has a paralog, LSO1, that arose from the whole genome duplication C IDA nucleus GO_0005634 2579 YGR170W PSD2 Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventually give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected C IDA endosome GO_0005768 2580 YGR171C MSM1 Mitochondrial methionyl-tRNA synthetase (MetRS) Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p C IMP mitochondrion GO_0005739 2581 YGR172C YIP1 Integral membrane protein Integral membrane protein; required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 C IDA; IPI; IDA; HDA endoplasmic reticulum membrane; ER to Golgi transport vesicle; integral component of Golgi membrane; endoplasmic reticulum GO_0005789; GO_0030134; GO_0030173; GO_0005783 2582 YGR173W RBG2 Protein with a role in translation Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein C HDA cytoplasm GO_0005737 2583 YGR174C CBP4 Mitochondrial protein required for assembly of cytochrome bc1 complex Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex C HDA; IDA mitochondrion; integral component of mitochondrial membrane GO_0005739; GO_0032592 2584 YGR174W-A YGR174W-A Putative protein of unknown function Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis 2585 YGR175C ERG1 Squalene epoxidase Squalene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine C IDA; HDA lipid particle; endoplasmic reticulum GO_0005811; GO_0005783 2586 YGR176W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2587 YGR177C ATF2 Alcohol acetyltransferase Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking C IDA; HDA; HDA integral component of endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm GO_0030176; GO_0005783; GO_0005737 2588 YGR178C PBP1 Component of glucose deprivation induced stress granules Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; HDA; HDA; HDA polysome; cytoplasmic stress granule; mitochondrion; cytoplasm; nucleus GO_0005844; GO_0010494; GO_0005739; GO_0005737; GO_0005634 2589 YGR179C OKP1 Outer kinetochore protein required for accurate chromosome segregation Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 C IDA; IDA condensed nuclear chromosome kinetochore; COMA complex GO_0000778; GO_0000817 2590 YGR180C RNR4 Ribonucleotide-diphosphate reductase (RNR) small subunit Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication C IPI; IDA; HDA ribonucleoside-diphosphate reductase complex; nucleus; cytoplasm GO_0005971; GO_0005634; GO_0005737 2591 YGR181W TIM13 Mitochondrial intermembrane space protein Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane C IDA; IPI; HDA mitochondrial intermembrane space; mitochondrial intermembrane space protein transporter complex; mitochondrion GO_0005758; GO_0042719; GO_0005739 2592 YGR182C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W 2593 YGR183C QCR9 Subunit 9 of ubiquinol cytochrome-c reductase (Complex III) Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex C IDA; HDA mitochondrial respiratory chain complex III; mitochondrion GO_0005750; GO_0005739 2594 YGR184C UBR1 E3 ubiquitin ligase (N-recognin) E3 ubiquitin ligase (N-recognin); heterodimerizes with Rad6p to ubiquitinate substrates in the N-end rule pathway; role in endoplasmic reticulum-associated protein degradation (ERAD) in the absence of canonical ER membrane ligases or after stress; major role in targeting misfolded cytosolic proteins for degradation; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) C IPI; IPI; IMP proteasome regulatory particle, base subcomplex; UBR1-RAD6 ubiquitin ligase complex; cytoplasm GO_0008540; GO_1990303; GO_0005737 2595 YGR185C TYS1 Cytoplasmic tyrosyl-tRNA synthetase Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA nucleus; cytoplasm; cytoplasmic stress granule GO_0005634; GO_0005737; GO_0010494 2596 YGR186W TFG1 TFIIF (Transcription Factor II) largest subunit TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 C IDA transcription factor TFIIF complex GO_0005674 2597 YGR187C HGH1 Nonessential protein of unknown function Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2598 YGR188C BUB1 Protein kinase involved in the cell cycle checkpoint into anaphase Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication C IDA; IDA; IDA; IDA nucleus; kinetochore; condensed nuclear chromosome, centromeric region; condensed nuclear chromosome kinetochore GO_0005634; GO_0000776; GO_0000780; GO_0000778 2599 YGR189C CRH1 Chitin transglycosylase Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress C HDA; IDA; IDA cell periphery; incipient cellular bud site; fungal-type cell wall GO_0071944; GO_0000131; GO_0009277 2600 YGR190C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W 2601 YGR191W HIP1 High-affinity histidine permease High-affinity histidine permease; also involved in the transport of manganese ions C HDA; HDA; IMP cell periphery; endoplasmic reticulum; plasma membrane GO_0071944; GO_0005783; GO_0005886 2602 YGR192C TDH3 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria; binds AU-rich RNA; TDH3 has a paralog, TDH2, that arose from the whole genome duplication C HDA; HDA; IDA; IDA; HDA; HDA; HDA nucleus; plasma membrane; fungal-type cell wall; lipid particle; mitochondrion; cytosol; cytoplasm GO_0005634; GO_0005886; GO_0009277; GO_0005811; GO_0005739; GO_0005829; GO_0005737 2603 YGR193C PDX1 E3-binding protein of the mitochondrial pyruvate dehydrogenase complex E3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core C IDA; HDA mitochondrial pyruvate dehydrogenase complex; mitochondrion GO_0005967; GO_0005739 2604 YGR194C XKS1 Xylulokinase Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains C HDA cytoplasm GO_0005737 2605 YGR195W SKI6 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) C IDA; IDA cytoplasmic exosome (RNase complex); nuclear exosome (RNase complex) GO_0000177; GO_0000176 2606 YGR196C FYV8 Protein of unknown function Protein of unknown function; required for survival upon exposure to K1 killer toxin C HDA cytoplasm GO_0005737 2607 YGR197C SNG1 Protein involved in resistance to nitrosoguanidine and 6-azauracil Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication C IDA; HDA; HDA plasma membrane; cell periphery; fungal-type vacuole GO_0005886; GO_0071944; GO_0000324 2608 YGR198W YPP1 Cargo-transport protein involved in endocytosis Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4p; is required, along with Efr3p, for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches at the plasma membrane; positively regulates Stt4p; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene C HDA; IDA; IDA; HDA; IDA; HDA; HDA nucleus; endosome; plasma membrane; cytosol; actin cortical patch; ribosome; cytoplasm GO_0005634; GO_0005768; GO_0005886; GO_0005829; GO_0030479; GO_0005840; GO_0005737 2609 YGR199W PMT6 Protein O-mannosyltransferase Protein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases C HDA endoplasmic reticulum GO_0005783 2610 YGR200C ELP2 Subunit of Elongator complex Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin C IDA; IDA; IDA cytoplasm; Elongator holoenzyme complex; nucleus GO_0005737; GO_0033588; GO_0005634 2611 YGR201C YGR201C Putative protein of unknown function Putative protein of unknown function 2612 YGR202C PCT1 Cholinephosphate cytidylyltransferase Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity C IDA; IDA nucleus; nuclear envelope GO_0005634; GO_0005635 2613 YGR203W YCH1 Phosphatase with sequence similarity to Cdc25p Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2614 YGR204C-A YGR204C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2615 YGR204W ADE3 Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2616 YGR205W TDA10 ATP-binding protein of unknown function ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2617 YGR206W MVB12 ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin C HDA; HDA; IPI cytosol; endosome; ESCRT I complex GO_0005829; GO_0005768; GO_0000813 2618 YGR207C CIR1 Mitochondrial protein that interacts with frataxin (Yfh1p) Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response C HDA mitochondrion GO_0005739 2619 YGR208W SER2 Phosphoserine phosphatase of the phosphoglycerate pathway Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2620 YGR209C TRX2 Cytoplasmic thioredoxin isoenzyme Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication C IPI; IDA fungal-type vacuole; cytosol GO_0000324; GO_0005829 2621 YGR210C YGR210C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2622 YGR211W ZPR1 Essential protein with two zinc fingers Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress C IDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2623 YGR212W SLI1 N-acetyltransferase N-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin C IDA; IDA nuclear envelope; plasma membrane GO_0005635; GO_0005886 2624 YGR213C RTA1 Protein involved in 7-aminocholesterol resistance Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication C IDA; HDA plasma membrane; endoplasmic reticulum GO_0005886; GO_0005783 2625 YGR214W RPS0A Ribosomal 40S subunit protein S0A Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; C IDA; IDA; HDA cytosolic small ribosomal subunit; cytoplasmic stress granule; 90S preribosome GO_0022627; GO_0010494; GO_0030686 2626 YGR215W RSM27 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 2627 YGR216C GPI1 Membrane protein involved in the synthesis of GlcNAc-PI Membrane protein involved in the synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs C IGI; HDA glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; endoplasmic reticulum GO_0000506; GO_0005783 2628 YGR217W CCH1 Voltage-gated high-affinity calcium channel Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function C IDA plasma membrane GO_0005886 2629 YGR218W CRM1 Major karyopherin Major karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin C IDA; IDA; IDA; HDA nucleus; spindle pole body; kinetochore; preribosome, large subunit precursor GO_0005634; GO_0005816; GO_0000776; GO_0030687 2630 YGR219W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 2631 YGR220C MRPL9 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 2632 YGR221C TOS2 Protein involved in localization of Cdc24p to the site of bud growth Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication C IDA; IDA; HDA; IDA cellular bud tip; cellular bud neck; cellular bud; incipient cellular bud site GO_0005934; GO_0005935; GO_0005933; GO_0000131 2633 YGR222W PET54 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing C IDA; IDA; IDA; HDA mitochondrial matrix; extrinsic component of mitochondrial inner membrane; mitochondrial inner membrane; mitochondrion GO_0005759; GO_0031314; GO_0005743; GO_0005739 2634 YGR223C HSV2 Phosphatidylinositol 3,5-bisphosphate-binding protein Phosphatidylinositol 3,5-bisphosphate-binding protein; plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization C IDA; IDA; IDA; IDA; HDA fungal-type vacuole; extrinsic component of membrane; cytoplasm; endosome; fungal-type vacuole membrane GO_0000324; GO_0019898; GO_0005737; GO_0005768; GO_0000329 2635 YGR224W AZR1 Plasma membrane transporter of the major facilitator superfamily Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 2636 YGR225W AMA1 Activator of meiotic anaphase promoting complex (APC/C) Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest C IPI anaphase-promoting complex GO_0005680 2637 YGR226C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W 2638 YGR227W DIE2 Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase; located in the ER; functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1 C HDA; HDA; IMP cell periphery; endoplasmic reticulum; endoplasmic reticulum membrane GO_0071944; GO_0005783; GO_0005789 2639 YGR228W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C 2640 YGR229C SMI1 Protein involved in the regulation of cell wall synthesis Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity C IDA; HDA; IDA incipient cellular bud site; mating projection tip; cellular bud neck GO_0000131; GO_0043332; GO_0005935 2641 YGR230W BNS1 Protein of unknown function Protein of unknown function; overexpression bypasses need for Spo12p, but not required for meiosis; BNS1 has a paralog, SPO12, that arose from the whole genome duplication 2642 YGR231C PHB2 Subunit of the prohibitin complex (Phb1p-Phb2p) Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; peroxisomal importomer complex GO_0005739; GO_0005743; GO_1990429 2643 YGR232W NAS6 Evolutionarily conserved 19S regulatory particle assembly-chaperone Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 C IDA; IDA; IDA proteasome regulatory particle; nucleus; cytosol GO_0005838; GO_0005634; GO_0005829 2644 YGR233C PHO81 Cyclin-dependent kinase (CDK) inhibitor Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; HDA cytosol; nucleus; cytoplasm GO_0005829; GO_0005634; GO_0005737 2645 YGR234W YHB1 Nitric oxide oxidoreductase Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C IDA; HDA; HDA; HDA; IDA; IDA cytoplasmic stress granule; mitochondrion; cytoplasm; nucleus; mitochondrial matrix; cytosol GO_0010494; GO_0005739; GO_0005737; GO_0005634; GO_0005759; GO_0005829 2646 YGR235C MIC26 Component of the MICOS complex Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex C IDA; HDA; IDA MICOS complex; mitochondrion; mitochondrial crista junction GO_0061617; GO_0005739; GO_0044284 2647 YGR236C SPG1 Protein required for high temperature survival during stationary phase Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 2648 YGR237C YGR237C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2649 YGR238C KEL2 Protein that negatively regulates mitotic exit Protein that negatively regulates mitotic exit; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; KEL2 has a paralog, KEL1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA mating projection tip; cellular bud neck; cellular bud tip; Kelch-containing formin regulatory complex GO_0043332; GO_0005935; GO_0005934; GO_1990615 2650 YGR239C PEX21 Peroxin required for peroxisomal matrix protein targeting Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress; PEX21 has a paralog, PEX18, that arose from the whole genome duplication C IDA; IDA; HDA peroxisome; cytosol; cytoplasm GO_0005777; GO_0005829; GO_0005737 2651 YGR240C PFK1 Alpha subunit of heterooctameric phosphofructokinase Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes C IDA; HDA; IPI; IDA 6-phosphofructokinase complex; cytoplasm; vacuolar proton-transporting V-type ATPase complex; mitochondrion GO_0005945; GO_0005737; GO_0016471; GO_0005739 2652 YGR240C-A YGR240C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 2653 YGR241C YAP1802 Protein of the AP180 family, involved in clathrin cage assembly Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication C HDA; HDA; HDA cytosol; prospore membrane; cellular bud neck GO_0005829; GO_0005628; GO_0005935 2654 YGR242W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C 2655 YGR243W MPC3 Highly conserved subunit of the mitochondrial pyruvate carrier (MPC) Highly conserved subunit of mitochondrial pyruvate carrier; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress; MPC3 has a paralog, MPC2, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial membrane GO_0005739; GO_0031966 2656 YGR244C LSC2 Beta subunit of succinyl-CoA ligase Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate C HDA mitochondrion GO_0005739 2657 YGR245C SDA1 Protein required for actin organization and passage through Start Protein required for actin organization and passage through Start; highly conserved nuclear protein; required for actin cytoskeleton organization; plays a critical role in G1 events; binds Nap1p; involved in 60S ribosome biogenesis C IDA nucleus GO_0005634 2658 YGR246C BRF1 TFIIIB B-related factor TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB C IDA transcription factor TFIIIB complex GO_0000126 2659 YGR247W CPD1 Cyclic nucleotide phosphodiesterase Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress C HDA clathrin-coated vesicle GO_0030136 2660 YGR248W SOL4 6-phosphogluconolactonase 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication C IDA; HDA; HDA cytosol; cytoplasm; nucleus GO_0005829; GO_0005737; GO_0005634 2661 YGR249W MGA1 Protein similar to heat shock transcription factor Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants C IDA nuclear chromatin GO_0000790 2662 YGR250C RIE1 Putative RNA binding protein Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress C IDA; HDA cytoplasmic stress granule; cytoplasm GO_0010494; GO_0005737 2663 YGR251W NOP19 Ribosome biogenesis factor Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes C HDA; IDA; IDA; IDA nucleolus; nucleus; nucleoplasm; 90S preribosome GO_0005730; GO_0005634; GO_0005654; GO_0030686 2664 YGR252W GCN5 Catalytic subunit of ADA and SAGA histone acetyltransferase complexes Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation C IDA; IDA; IDA; IDA; IDA; IDA Ada2/Gcn5/Ada3 transcription activator complex; SAGA complex; cytosol; SLIK (SAGA-like) complex; chromosome, centromeric region; nucleus GO_0005671; GO_0000124; GO_0005829; GO_0046695; GO_0000775; GO_0005634 2665 YGR253C PUP2 Alpha 5 subunit of the 20S proteasome Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta C IDA; IDA proteasome core complex, alpha-subunit complex; proteasome storage granule GO_0019773; GO_0034515 2666 YGR254W ENO1 Enolase I, a phosphopyruvate hydratase Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication C IDA; IDA; HDA; HDA; HDA; HDA phosphopyruvate hydratase complex; fungal-type vacuole; plasma membrane; cytosol; cytoplasm; mitochondrion GO_0000015; GO_0000324; GO_0005886; GO_0005829; GO_0005737; GO_0005739 2667 YGR255C COQ6 Flavin-dependent monooxygenase involved in ubiquinone biosynthesis Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 2668 YGR256W GND2 6-phosphogluconate dehydrogenase (decarboxylating) 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication C HDA plasma membrane GO_0005886 2669 YGR257C MTM1 Mitochondrial protein of the mitochondrial carrier family Mitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5′-phosphate transporter; involved in mitochondrial iron homeostasis and in activating mitochondrial Sod2p by facilitating insertion of an essential manganese cofactor C IDA mitochondrion GO_0005739 2670 YGR258C RAD2 Single-stranded DNA endonuclease Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein C IDA nucleotide-excision repair factor 3 complex GO_0000112 2671 YGR259C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W 2672 YGR260W TNA1 High affinity nicotinic acid plasma membrane permease High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) C HDA; HDA; HDA mitochondrion; fungal-type vacuole; cell periphery GO_0005739; GO_0000324; GO_0071944 2673 YGR261C APL6 Beta3-like subunit of the yeast AP-3 complex Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools C HDA; IMP cytosol; AP-3 adaptor complex GO_0005829; GO_0030123 2674 YGR262C BUD32 Protein kinase Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription C IDA; HDA; HDA EKC/KEOPS complex; nucleus; cytoplasm GO_0000408; GO_0005634; GO_0005737 2675 YGR263C SAY1 Sterol deacetylase Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER C HDA; IDA; IDA; HDA; IDA endoplasmic reticulum; endoplasmic reticulum lumen; integral component of membrane; cell periphery; lipid particle GO_0005783; GO_0005788; GO_0016021; GO_0071944; GO_0005811 2676 YGR264C MES1 Methionyl-tRNA synthetase Methionyl-tRNA synthetase; forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs C IDA; IDA cytoplasm; methionyl glutamyl tRNA synthetase complex GO_0005737; GO_0017102 2677 YGR265W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase 2678 YGR266W YGR266W Protein of unknown function Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress C HDA; HDA; IDA; HDA mitochondrion; prospore membrane; plasma membrane; mitochondrial outer membrane GO_0005739; GO_0005628; GO_0005886; GO_0005741 2679 YGR267C FOL2 GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesis GTP-cyclohydrolase I; catalyzes the first step in the folic acid biosynthetic pathway C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2680 YGR268C HUA1 Cytoplasmic protein containing a zinc finger domain Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly C IDA cytoplasm GO_0005737 2681 YGR269W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C 2682 YGR270C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YTA7/YGR270W 2683 YGR270W YTA7 Protein that localizes to chromatin Protein that localizes to chromatin; has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; relocalizes to the cytosol in response to hypoxia; potentially phosphorylated by Cdc28p C IDA; IDA; IDA nucleus; cytosol; extrinsic component of endoplasmic reticulum membrane GO_0005634; GO_0005829; GO_0042406 2684 YGR271C-A EFG1 Essential protein required for maturation of 18S rRNA Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus C IDA; HDA preribosome, small subunit precursor; nucleolus GO_0030688; GO_0005730 2685 YGR271W SLH1 Putative RNA helicase related to Ski2p Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses C IDA; HDA; IDA polysomal ribosome; cytoplasm; cytoplasmic stress granule GO_0042788; GO_0005737; GO_0010494 2686 YGR273C YGR273C Putative protein of unknown function Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication 2687 YGR274C TAF1 TFIID subunit, involved in RNA pol II transcription initiation TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA transcription factor TFIID complex; nucleus; cytosol GO_0005669; GO_0005634; GO_0005829 2688 YGR275W RTT102 Component of both the SWI/SNF and RSC chromatin remodeling complexes Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA SWI/SNF complex; RSC complex; nucleus GO_0016514; GO_0016586; GO_0005634 2689 YGR276C RNH70 3'-5' exoribonuclease 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts C IDA nucleus GO_0005634 2690 YGR277C CAB4 Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved C IDA; HDA; HDA CoA-synthesizing protein complex; nucleus; cytoplasm GO_1990143; GO_0005634; GO_0005737 2691 YGR278W CWC22 Spliceosome-associated protein that is required for pre-mRNA splicing Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein C IDA; IDA U2-type catalytic step 1 spliceosome; U2-type spliceosomal complex GO_0071006; GO_0005684 2692 YGR279C SCW4 Cell wall protein with similarity to glucanases Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication C IDA; IDA; HDA; IDA fungal-type cell wall; extracellular region; fungal-type vacuole; vacuole GO_0009277; GO_0005576; GO_0000324; GO_0005773 2693 YGR280C PXR1 Essential protein involved in rRNA and snoRNA maturation Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain C IDA; IDA nucleolus; nucleoplasm GO_0005730; GO_0005654 2694 YGR281W YOR1 Plasma membrane ATP-binding cassette (ABC) transporter Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR) C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 2695 YGR282C BGL2 Endo-beta-1,3-glucanase Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall C IDA; HDA fungal-type cell wall; fungal-type vacuole GO_0009277; GO_0000324 2696 YGR283C YGR283C Putative methyltransferase Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication C HDA; HDA ribosome; nucleolus GO_0005840; GO_0005730 2697 YGR284C ERV29 Protein localized to COPII-coated vesicles Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress C HDA; IDA endoplasmic reticulum; ER to Golgi transport vesicle GO_0005783; GO_0030134 2698 YGR285C ZUO1 Ribosome-associated chaperone Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p C HDA; HDA; IMP; IDA; IDA mitochondrion; cytoplasm; nucleolus; ribosome; polysome GO_0005739; GO_0005737; GO_0005730; GO_0005840; GO_0005844 2699 YGR286C BIO2 Biotin synthase Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant C HDA mitochondrion GO_0005739 2700 YGR287C IMA1 Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase) Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family C HDA; HDA mitochondrion; ribosome GO_0005739; GO_0005840 2701 YGR288W MAL13 MAL-activator protein MAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C 2702 YGR289C MAL11 High-affinity maltose transporter (alpha-glucoside transporter) High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization 2703 YGR290W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) 2704 YGR291C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2705 YGR292W MAL12 Maltase (alpha-D-glucosidase) Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose C HDA plasma membrane GO_0005886 2706 YGR293C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR294W 2707 YGR294W PAU12 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions 2708 YGR295C COS6 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C IDA; HDA endosome; fungal-type vacuole GO_0005768; GO_0000324 2709 YGR296C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL07R 2710 YGR296C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; contained completely within TEL07R 2711 YGR296W YRF1-3 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 2712 YHL001W RPL14B Ribosomal 60S subunit protein L14B Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 2713 YHL002C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF HSE1/YHL002W 2714 YHL002W HSE1 Subunit of the endosomal Vps27p-Hse1p complex Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes C IPI; IDA ESCRT-0 complex; endosome GO_0033565; GO_0005768 2715 YHL003C LAG1 Ceramide synthase component Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enahnces invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; acyl-CoA ceramide synthase complex GO_0005783; GO_0061576 2716 YHL004W MRP4 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 2717 YHL005C YHL005C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W 2718 YHL006C SHU1 Component of Shu complex (aka PCSS complex) Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments C IDA; IPI; IMP nucleolus; Shu complex; site of double-strand break GO_0005730; GO_0097196; GO_0035861 2719 YHL006W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF STE20/YHL007C 2720 YHL007C STE20 Cdc42p-activated signal transducing kinase Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family C IDA; IDA; IDA; HDA; IDA incipient cellular bud site; nucleus; cytoplasm; plasma membrane; mating projection tip GO_0000131; GO_0005634; GO_0005737; GO_0005886; GO_0043332 2721 YHL008C YHL008C Putative protein of unknown function Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole C HDA fungal-type vacuole GO_0000324 2722 YHL009C YAP3 Basic leucine zipper (bZIP) transcription factor Basic leucine zipper (bZIP) transcription factor 2723 YHL009W-A YHL009W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 2724 YHL009W-B YHL009W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 2725 YHL010C ETP1 Protein of unknown function required for growth on ethanol Putative protein of unknown function required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences 2726 YHL011C PRS3 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes C HDA; IDA cytoplasm; ribose phosphate diphosphokinase complex GO_0005737; GO_0002189 2727 YHL012W YHL012W Putative UTP glucose-1-phosphate uridylyltransferase Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication 2728 YHL013C OTU2 Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; ribosome GO_0005737; GO_0005840 2729 YHL014C YLF2 Protein of unknown function Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 2730 YHL015W RPS20 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 C IDA cytosolic small ribosomal subunit GO_0022627 2731 YHL015W-A YHL015W-A Putative protein of unknown function Putative protein of unknown function 2732 YHL016C DUR3 Plasma membrane transporter for both urea and polyamines Plasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway C HDA; HDA; IDA endoplasmic reticulum; cell periphery; plasma membrane GO_0005783; GO_0071944; GO_0005886 2733 YHL017W YHL017W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication C HDA clathrin-coated vesicle GO_0030136 2734 YHL018W MCO14 Putative 4a-hydroxytetrahydrobiopterin dehydratase Putative 4a-hydroxytetrahydrobiopterin dehydratase; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS C HDA mitochondrion GO_0005739 2735 YHL019C APM2 Protein of unknown function Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport C IPI; HDA AP-1 adaptor complex; nucleus GO_0030121; GO_0005634 2736 YHL019W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene APM2/YHL019C 2737 YHL020C OPI1 Transcriptional regulator of a variety of genes Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol C IDA; IDA; IDA; IDA; HDA; IDA nucleus; endoplasmic reticulum; nuclear membrane; nucleoplasm; nuclear periphery; nuclear envelope GO_0005634; GO_0005783; GO_0031965; GO_0005654; GO_0034399; GO_0005635 2738 YHL021C AIM17 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 2739 YHL022C SPO11 Meiosis-specific protein that initiates meiotic recombination Meiosis-specific protein that initiates meiotic recombination; initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation C IPI nuclear chromosome GO_0000228 2740 YHL023C NPR3 Subunit of the Iml1p/SEACIT complex Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair C IDA; IDA; IDA extrinsic component of fungal-type vacuolar membrane; GATOR1 complex; Seh1-associated complex GO_0097042; GO_1990130; GO_0035859 2741 YHL024W RIM4 Putative RNA-binding protein Putative RNA-binding protein; required for the expression of early and middle sporulation genes C HDA cytoplasm GO_0005737 2742 YHL025W SNF6 Subunit of the SWI/SNF chromatin remodeling complex Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions C IDA; IDA; IDA SWI/SNF complex; cytosol; nucleus GO_0016514; GO_0005829; GO_0005634 2743 YHL026C YHL026C Putative protein of unknown function Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) C HDA cell periphery GO_0071944 2744 YHL027W RIM101 Cys2His2 zinc-finger transcriptional repressor Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC C IDA nucleus GO_0005634 2745 YHL028W WSC4 Endoplasmic reticulum (ER) membrane protein Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 C IDA endoplasmic reticulum membrane GO_0005789 2746 YHL029C OCA5 Cytoplasmic protein required for replication of Brome mosaic virus Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts C HDA cytoplasm GO_0005737 2747 YHL030W ECM29 Scaffold protein Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress C IDA; IDA; HDA proteasome complex; nucleus; cytoplasm GO_0000502; GO_0005634; GO_0005737 2748 YHL030W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene GOS1/YHL031C 2749 YHL031C GOS1 v-SNARE protein involved in Golgi transport v-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28 C IDA; IDA SNARE complex; Golgi medial cisterna GO_0031201; GO_0005797 2750 YHL032C GUT1 Glycerol kinase Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p C IDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2751 YHL033C RPL8A Ribosomal 60S subunit protein L8A Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2752 YHL034C SBP1 Protein that binds eIF4G and has a role in repression of translation Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cytoplasm; nucleolus; cytoplasmic stress granule; P-body GO_0005737; GO_0005730; GO_0010494; GO_0000932 2753 YHL034W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SBP1/YHL034C 2754 YHL035C VMR1 Vacuolar membrane protein Vacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication C HDA; IDA; HDA mitochondrion; fungal-type vacuole membrane; ribosome GO_0005739; GO_0000329; GO_0005840 2755 YHL036W MUP3 Low affinity methionine permease Low affinity methionine permease; similar to Mup1p C HDA fungal-type vacuole GO_0000324 2756 YHL037C YHL037C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2757 YHL038C CBP2 Required for splicing of the group I intron bI5 of the COB pre-mRNA Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene C IDA mitochondrion GO_0005739 2758 YHL039W EFM1 Lysine methyltransferase Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2759 YHL040C ARN1 ARN family transporter for siderophore-iron chelates ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress C IDA; IDA; HDA; IDA; HDA; HDA; HDA cytoplasmic vesicle; plasma membrane; cell periphery; endosome; nucleus; cytoplasm; fungal-type vacuole GO_0031410; GO_0005886; GO_0071944; GO_0005768; GO_0005634; GO_0005737; GO_0000324 2760 YHL041W YHL041W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2761 YHL042W YHL042W Protein of unknown function Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA fungal-type vacuole GO_0000324 2762 YHL043W ECM34 Protein of unknown function Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA cytosol GO_0005829 2763 YHL044W YHL044W Putative integral membrane protein Putative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern C IMP plasma membrane GO_0005886 2764 YHL045W Putative protein of unknown function; not an essential gene 2765 YHL046C PAU13 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock 2766 YHL046W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARN2/YHL047C 2767 YHL047C ARN2 Transporter Transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C C HDA fungal-type vacuole GO_0000324 2768 YHL048C-A YHL048C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 2769 YHL048W COS8 Endosomal protein involved in turnover of plasma membrane proteins Nuclear membrane protein; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response C IDA; HDA nuclear envelope; fungal-type vacuole GO_0005635; GO_0000324 2770 YHL049C YHL049C Putative protein of unknown function Putative protein of unknown function 2771 YHL050C YHL050C Putative protein of unknown function Putative protein of unknown function; potential Cdc28p substrate 2772 YHL050W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2773 YHR001W OSH7 Oxysterol-binding protein Oxysterol-binding protein; part of family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH7 has a paralog, OSH6, that arose from the whole genome duplication C IDA; HDA; IDA cytoplasm; cytosol; cortical endoplasmic reticulum GO_0005737; GO_0005829; GO_0032541 2774 YHR001W-A QCR10 Subunit of the ubiqunol-cytochrome c oxidoreductase complex Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain C HDA; IDA mitochondrion; mitochondrial respiratory chain complex III GO_0005739; GO_0005750 2775 YHR002W LEU5 Mitochondrial carrier protein Mitochondrial carrier protein; involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 2776 YHR003C TCD1 tRNA threonylcarbamoyladenosine dehydratase tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 2777 YHR004C NEM1 Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard C IDA; HDA; IPI; IDA integral component of membrane; mitochondrion; Nem1-Spo7 phosphatase complex; lipid particle GO_0016021; GO_0005739; GO_0071595; GO_0005811 2778 YHR005C GPA1 Subunit of the G protein involved in pheromone response Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; HDA heterotrimeric G-protein complex; plasma membrane; endosome; cytosol GO_0005834; GO_0005886; GO_0005768; GO_0005829 2779 YHR005C-A TIM10 Essential protein of the mitochondrial intermembrane space Essential protein of the mitochondrial intermembrane space; forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane C IDA; HDA mitochondrial intermembrane space protein transporter complex; mitochondrion GO_0042719; GO_0005739 2780 YHR006W STP2 Transcription factor Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2781 YHR007C ERG11 Lanosterol 14-alpha-demethylase Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p C HDA endoplasmic reticulum GO_0005783 2782 YHR007C-A YHR007C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 2783 YHR008C SOD2 Mitochondrial manganese superoxide dismutase Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity; phosphorylated C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 2784 YHR009C TDA3 Putative oxidoreductase involved in late endosome to Golgi transport Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 C IDA; IDA cytoplasm; late endosome GO_0005737; GO_0005770 2785 YHR010W RPL27A Ribosomal 60S subunit protein L27A Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2786 YHR011W DIA4 Probable mitochondrial seryl-tRNA synthetase Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth C IMP; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2787 YHR012W VPS29 Subunit of the membrane-associated retromer complex Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval C IPI; IPI; IPI retromer complex; retromer, cargo-selective complex; endosome GO_0030904; GO_0030906; GO_0005768 2788 YHR013C ARD1 Subunit of protein N-terminal acetyltransferase NatA Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress C IDA; IDA cytosolic ribosome; NatA complex GO_0022626; GO_0031415 2789 YHR014W SPO13 Meiotic regulator Meiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells C IDA; IDA; IDA meiotic spindle; condensed nuclear chromosome, centromeric region; nucleus GO_0072687; GO_0000780; GO_0005634 2790 YHR015W MIP6 Putative RNA-binding protein Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication 2791 YHR016C YSC84 Actin-binding protein Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication C IDA; HDA actin cortical patch; cytosol GO_0030479; GO_0005829 2792 YHR017W YSC83 Non-essential mitochondrial protein of unknown function Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 C HDA; HDA mitochondrial outer membrane; mitochondrion GO_0005741; GO_0005739 2793 YHR018C ARG4 Argininosuccinate lyase Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway C IDA cytosol GO_0005829 2794 YHR019C DED81 Cytosolic asparaginyl-tRNA synthetase Cytosolic asparaginyl-tRNA synthetase; required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA 2795 YHR020W YHR020W Prolyl-tRNA synthetase Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments C HDA ribosome GO_0005840 2796 YHR021C RPS27B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 2797 YHR021W-A ECM12 Putative protein of unknown function Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity 2798 YHR022C YHR022C Putative protein of unknown function Putative protein of unknown function; YHR022C is not an essential gene 2799 YHR022C-A YHR022C-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2800 YHR023W MYO1 Type II myosin heavy chain Type II myosin heavy chain; required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively C IDA; IDA; IPI; IDA incipient cellular bud site; cellular bud neck contractile ring; myosin II complex; cellular bud neck GO_0000131; GO_0000142; GO_0016460; GO_0005935 2801 YHR024C MAS2 Alpha subunit of the mitochondrial processing protease (MPP) Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins C IDA; HDA mitochondrial processing peptidase complex; mitochondrion GO_0017087; GO_0005739 2802 YHR025W THR1 Homoserine kinase Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway 2803 YHR026W VMA16 Subunit c'' of the vacuolar ATPase Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain C IDA integral component of membrane GO_0016021 2804 YHR027C RPN1 Non-ATPase base subunit of the 19S RP of the 26S proteasome Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle C IDA; IDA; HDA; HDA; IDA proteasome storage granule; nucleus; cytoplasm; endoplasmic reticulum; proteasome regulatory particle, base subcomplex GO_0034515; GO_0005634; GO_0005737; GO_0005783; GO_0008540 2805 YHR028C DAP2 Dipeptidyl aminopeptidase Dipeptidyl aminopeptidase; synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p C HDA fungal-type vacuole membrane GO_0000329 2806 YHR028W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF DAP2/YHR028C 2807 YHR029C YHI9 Protein of unknown function Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production 2808 YHR030C SLT2 Serine/threonine MAP kinase Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to phagophore assembly site (PAS); plays a role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway; SLT2 has a paralog, KDX1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; IDA; HDA; IDA cellular bud tip; cellular bud neck; cytoplasm; nucleus; mating projection tip; cytosol; mitochondrion GO_0005934; GO_0005935; GO_0005737; GO_0005634; GO_0043332; GO_0005829; GO_0005739 2809 YHR031C RRM3 DNA helicase involved in rDNA replication and Ty1 transposition DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p C IDA; IPI; IDA replication fork; nuclear telomeric heterochromatin; nuclear replication fork GO_0005657; GO_0005724; GO_0043596 2810 YHR032C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032W-A 2811 YHR032W ERC1 Member of the multi-drug and toxin extrusion (MATE) family Member of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine 2812 YHR032W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032C-A 2813 YHR033W YHR033W Putative protein of unknown function Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 2814 YHR034C PIH1 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1) Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II C IPI; IPI; HDA; HDA small nucleolar ribonucleoprotein complex; R2TP complex; cytoplasm; nucleus GO_0005732; GO_0097255; GO_0005737; GO_0005634 2815 YHR035W NEL1 Activator of Sar1p GTPase activity Activator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene C IDA cytosol GO_0005829 2816 YHR036W BRL1 Essential nuclear envelope/ER integral membrane protein Essential nuclear envelope integral membrane protein; identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export C IDA; IDA; IDA nuclear envelope; integral component of membrane; endoplasmic reticulum GO_0005635; GO_0016021; GO_0005783 2817 YHR037W PUT2 Delta-1-pyrroline-5-carboxylate dehydrogenase Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 2818 YHR038W RRF1 Mitochondrial ribosome recycling factor Mitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria C HDA mitochondrion GO_0005739 2819 YHR039C MSC7 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids C HDA endoplasmic reticulum GO_0005783 2820 YHR039C-A VMA10 Subunit G of the V1 peripheral membrane domain of V-ATPase Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits C HDA; IDA fungal-type vacuole membrane; vacuolar proton-transporting V-type ATPase, V1 domain GO_0000329; GO_0000221 2821 YHR040W BCD1 Essential protein required for the accumulation of box C/D snoRNA Essential protein required for the accumulation of box C/D snoRNA C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2822 YHR041C SRB2 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance C IDA core mediator complex GO_0070847 2823 YHR042W NCP1 NADP-cytochrome P450 reductase NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p C HDA; HDA mitochondrial outer membrane; mitochondrion GO_0005741; GO_0005739 2824 YHR043C DOG2 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2825 YHR044C DOG1 2-deoxyglucose-6-phosphate phosphatase 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae 2826 YHR045W YHR045W Putative protein of unknown function Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum C HDA endoplasmic reticulum GO_0005783 2827 YHR046C INM1 Inositol monophosphatase Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2828 YHR047C AAP1 Arginine/alanine amino peptidase Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2829 YHR048W YHK8 Presumed antiporter of the major facilitator superfamily Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles C HDA cell periphery GO_0071944 2830 YHR049C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2831 YHR049W FSH1 Putative serine hydrolase Putative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2832 YHR050W SMF2 Divalent metal ion transporter involved in manganese homeostasis Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins C IDA; HDA; IDA trans-Golgi network; mitochondrion; late endosome GO_0005802; GO_0005739; GO_0005770 2833 YHR050W-A YHR050W-A Protein of unknown function Protein of unknown function; identified by expression profiling and mass spectrometry 2834 YHR051W COX6 Subunit VI of cytochrome c oxidase (Complex IV) Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 2835 YHR052W CIC1 Essential protein that interacts with proteasome components Essential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles C IPI; IDA; HDA proteasome complex; nucleolus; preribosome, large subunit precursor GO_0000502; GO_0005730; GO_0030687 2836 YHR052W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CUP1-1 2837 YHR053C CUP1-1 Metallothionein Metallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-1 has a paralog, CUP1-2, that arose from a segmental duplication C IDA cytosol GO_0005829 2838 YHR054C YHR054C Putative protein of unknown function Putative protein of unknown function; partial duplicate of RSC30/YHR056C, truncated remnant of segmental duplication 2839 YHR054W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CUP1-2 2840 YHR055C CUP1-2 Metallothionein Metallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-2 has a paralog, CUP1-1, that arose from a segmental duplication C IDA cytosol GO_0005829 2841 YHR056C RSC30 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; null mutants are osmosensitive; RSC30 has a paralog, RSC3, that arose from the whole genome duplication C IDA RSC complex GO_0016586 2842 YHR056W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF RSC30/YHR056C 2843 YHR057C CPR2 Peptidyl-prolyl cis-trans isomerase (cyclophilin) Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR2 has a paralog, CPR5, that arose from the whole genome duplication; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema C HDA fungal-type vacuole GO_0000324 2844 YHR058C MED6 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress C IDA core mediator complex GO_0070847 2845 YHR059W FYV4 Protein of unknown function Protein of unknown function; required for survival upon exposure to K1 killer toxin C IDA mitochondrion GO_0005739 2846 YHR060W VMA22 Protein that is required for vacuolar H+-ATPase (V-ATPase) function Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) C HDA; IMP; IPI nucleus; extrinsic component of endoplasmic reticulum membrane; Vma12-Vma22 assembly complex GO_0005634; GO_0042406; GO_1990871 2847 YHR061C GIC1 Protein involved in initiation of budding and cellular polarization Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication C IDA; IDA; IDA; HDA; HDA; IDA mating projection tip; cellular bud tip; cellular bud neck; nucleus; cytoplasm; incipient cellular bud site GO_0043332; GO_0005934; GO_0005935; GO_0005634; GO_0005737; GO_0000131 2848 YHR062C RPP1 Subunit of both RNase MRP and nuclear RNase P Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IPI ribonuclease MRP complex; nucleus; cytosol; nucleolar ribonuclease P complex GO_0000172; GO_0005634; GO_0005829; GO_0005655 2849 YHR063C PAN5 2-dehydropantoate 2-reductase 2-dehydropantoate 2-reductase; part of the pantothenic acid pathway, structurally homologous to E. coli panE C HDA cytoplasm GO_0005737 2850 YHR063W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SSZ1/YHR064C 2851 YHR064C SSZ1 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP C IDA; IDA polysome; cytoplasm GO_0005844; GO_0005737 2852 YHR065C RRP3 Protein involved in rRNA processing Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity 2853 YHR066W SSF1 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication C IDA; IDA preribosome, large subunit precursor; nucleolus GO_0030687; GO_0005730 2854 YHR067W HTD2 Mitochondrial 3-hydroxyacyl-thioester dehydratase Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology C HDA mitochondrion GO_0005739 2855 YHR068W DYS1 Deoxyhypusine synthase Deoxyhypusine synthase; catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric C HDA cytoplasm GO_0005737 2856 YHR069C RRP4 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) C IDA; IDA cytoplasmic exosome (RNase complex); nuclear exosome (RNase complex) GO_0000177; GO_0000176 2857 YHR069C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF TRM5/YHR070W 2858 YHR070C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene TRM5/YHR070W 2859 YHR070W TRM5 tRNA(m(1)G37)methyltransferase tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya C HDA; IDA; HDA cytoplasm; mitochondrial matrix; nucleus GO_0005737; GO_0005759; GO_0005634 2860 YHR071C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ERG7/YHR072W 2861 YHR071W PCL5 Cyclin Cyclin; interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity C IPI; IDA cyclin-dependent protein kinase holoenzyme complex; nucleus GO_0000307; GO_0005634 2862 YHR072W ERG7 Lanosterol synthase Lanosterol synthase; an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis C IDA lipid particle GO_0005811 2863 YHR072W-A NOP10 Subunit of box H/ACA snoRNP complex Subunit of box H/ACA snoRNP complex; required for pseudouridylation and processing of pre-18S rRNA C IPI box H/ACA snoRNP complex GO_0031429 2864 YHR073C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry 2865 YHR073W OSH3 Member of an oxysterol-binding protein family Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability C IDA; IDA; IDA; HDA cytoplasm; cortical endoplasmic reticulum; plasma membrane; cytosol GO_0005737; GO_0032541; GO_0005886; GO_0005829 2866 YHR073W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry 2867 YHR074W QNS1 Glutamine-dependent NAD(+) synthetase Glutamine-dependent NAD(+) synthetase; essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2868 YHR075C PPE1 Protein with carboxyl methyl esterase activity Protein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein C IDA mitochondrial small ribosomal subunit GO_0005763 2869 YHR076W PTC7 Type 2C serine/threonine protein phosphatase (PP2C) Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p C IDA; IDA nuclear envelope; mitochondrion GO_0005635; GO_0005739 2870 YHR077C NMD2 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance C IDA; IDA cytoplasm; polysome GO_0005737; GO_0005844 2871 YHR078W YHR078W High osmolarity-regulated gene of unknown function High osmolarity-regulated gene of unknown function 2872 YHR079C IRE1 Serine-threonine kinase and endoribonuclease Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress C IDA; IDA; HDA nucleus; integral component of endoplasmic reticulum membrane; endoplasmic reticulum GO_0005634; GO_0030176; GO_0005783 2873 YHR079C-A SAE3 Meiosis-specific protein involved in meiotic recombination Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p C IDA condensed nuclear chromosome GO_0000794 2874 YHR080C LAM4 Sterol-binding protein that localizes to puncta in the cortical ER Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication C HDA; HDA; IDA mitochondrion; ribosome; cortical endoplasmic reticulum GO_0005739; GO_0005840; GO_0032541 2875 YHR081W LRP1 Nuclear exosome-associated nucleic acid binding protein Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination C IDA; IDA nuclear exosome (RNase complex); nucleus GO_0000176; GO_0005634 2876 YHR082C KSP1 Serine/threonine protein kinase Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA cytoplasm; nucleus; cytoplasmic stress granule GO_0005737; GO_0005634; GO_0010494 2877 YHR083W SAM35 Component of the sorting and assembly machinery (SAM) complex Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane C HDA; IDA; IMP mitochondrion; mitochondrial outer membrane; mitochondrial sorting and assembly machinery complex GO_0005739; GO_0005741; GO_0001401 2878 YHR084W STE12 Transcription factor that is activated by a MAPK signaling cascade Transcription factor that is activated by a MAPK signaling cascade; activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth C IDA; IDA; IDA; IDA Tec1p-Ste12p-Dig1p complex; Ste12p-Dig1p-Dig2p complex; nucleus; nucleoplasm GO_1990527; GO_1990526; GO_0005634; GO_0005654 2879 YHR085W IPI1 Component of the Rix1 complex and possibly pre-replicative complexes Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene C HDA; IDA; HDA; IDA; IDA nucleus; nucleoplasm; preribosome, large subunit precursor; Rix1 complex; cytosol GO_0005634; GO_0005654; GO_0030687; GO_0097344; GO_0005829 2880 YHR086W NAM8 RNA binding protein, component of the U1 snRNP protein RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function C IDA; IDA; IPI; IDA; IDA U2-type prespliceosome; U1 snRNP; commitment complex; cytoplasm; nucleus GO_0071004; GO_0005685; GO_0000243; GO_0005737; GO_0005634 2881 YHR086W-A YHR086W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 2882 YHR087W RTC3 Protein of unknown function involved in RNA metabolism Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2883 YHR088W RPF1 Protein involved in assembly and export of the large ribosomal subunit Protein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA C IPI; HDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 2884 YHR089C GAR1 Protein component of the H/ACA snoRNP pseudouridylase complex Protein component of the H/ACA snoRNP pseudouridylase complex; involved in the modification and cleavage of the 18S pre-rRNA C IPI; IPI; IDA small nucleolar ribonucleoprotein complex; box H/ACA snoRNP complex; nucleolus GO_0005732; GO_0031429; GO_0005730 2885 YHR090C YNG2 Subunit of NuA4, an essential histone acetyltransferase complex Subunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; relocalizes to the cytosol in response to hypoxia; similar to human tumor suppressor ING1 and its isoforms ING4 and ING5 C IDA; IDA; IDA; IDA nucleus; cytosol; Piccolo NuA4 histone acetyltransferase complex; NuA4 histone acetyltransferase complex GO_0005634; GO_0005829; GO_0032777; GO_0035267 2886 YHR091C MSR1 Mitochondrial arginyl-tRNA synthetase Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 2887 YHR092C HXT4 High-affinity glucose transporter High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 2888 YHR093W AHT1 Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region 2889 YHR094C HXT1 Low-affinity glucose transporter of the major facilitator superfamily Low-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 2890 YHR095W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2891 YHR096C HXT5 Hexose transporter with moderate affinity for glucose Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication C HDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 2892 YHR097C YHR097C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication C HDA; HDA; IDA nucleus; cytoplasm; nuclear periphery GO_0005634; GO_0005737; GO_0034399 2893 YHR098C SFB3 Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat; COPII coat is required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p C HDA; IDA; HDA; HDA mating projection tip; COPII vesicle coat; cell periphery; endoplasmic reticulum GO_0043332; GO_0030127; GO_0071944; GO_0005783 2894 YHR099W TRA1 Subunit of SAGA and NuA4 histone acetyltransferase complexes Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation C HDA; IDA; IDA; IDA; IDA ASTRA complex; NuA4 histone acetyltransferase complex; SLIK (SAGA-like) complex; nucleus; SAGA complex GO_0070209; GO_0035267; GO_0046695; GO_0005634; GO_0000124 2895 YHR100C GEP4 Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase) Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase); dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 2896 YHR101C BIG1 Integral membrane protein of the endoplasmic reticulum Integral membrane protein of the endoplasmic reticulum; required for normal content of cell wall beta-1,6-glucan C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 2897 YHR102W KIC1 Protein kinase of the PAK/Ste20 family, required for cell integrity Protein kinase of the PAK/Ste20 family, required for cell integrity; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; part of the RAM network that regulates cellular polarity and morphogenesis C HDA; IDA; IDA; IDA cytoplasm; cellular bud; mating projection tip; incipient cellular bud site GO_0005737; GO_0005933; GO_0043332; GO_0000131 2898 YHR103W SBE22 Protein involved in bud growth Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 2899 YHR104W GRE3 Aldose reductase Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2900 YHR105W YPT35 Endosomal protein of unknown function Endosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport C HDA endosome GO_0005768 2901 YHR106W TRR2 Mitochondrial thioredoxin reductase Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 2902 YHR107C CDC12 Component of the septin ring that is required for cytokinesis Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells C IDA; IPI; HDA; HDA; IDA; IDA; IDA; HDA cellular bud scar; septin complex; prospore membrane; cytoplasm; mating projection; cellular bud neck septin ring; septin filament array; cellular bud neck GO_0005621; GO_0031105; GO_0005628; GO_0005737; GO_0005937; GO_0000144; GO_0032160; GO_0005935 2903 YHR108W GGA2 Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication C IDA trans-Golgi network GO_0005802 2904 YHR109W CTM1 Cytochrome c lysine methyltransferase Cytochrome c lysine methyltransferase; trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth C IDA cytosol GO_0005829 2905 YHR110W ERP5 Protein with similarity to Emp24p and Erv25p Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport C HDA endoplasmic reticulum GO_0005783 2906 YHR111W UBA4 E1-like protein that activates Urm1p before urmylation E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p C HDA cytoplasm GO_0005737 2907 YHR112C YHR112C Protein of unknown function Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 2908 YHR113W APE4 Cytoplasmic aspartyl aminopeptidase with possible vacuole function Cytoplasmic aspartyl aminopeptidase with possible vacuole function; Cvt pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family C HDA; IDA; HDA cytoplasm; fungal-type vacuole lumen; ribosome GO_0005737; GO_0000328; GO_0005840 2909 YHR114W BZZ1 SH3 domain protein implicated in regulating actin polymerization SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins C IDA; HDA; HDA; IDA; HDA actin cortical patch; cellular bud neck; cytoplasm; plasma membrane; mating projection tip GO_0030479; GO_0005935; GO_0005737; GO_0005886; GO_0043332 2910 YHR115C DMA1 Ubiquitin-protein ligase (E3) Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 2911 YHR116W COX23 Protein that functions in mitochondrial copper homeostasis Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs C IDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2912 YHR117W TOM71 Mitochondrial outer membrane protein Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication C HDA; IDA; IDA mitochondrion; mitochondrial outer membrane; integral component of mitochondrial outer membrane GO_0005739; GO_0005741; GO_0031307 2913 YHR118C ORC6 Subunit of the origin recognition complex (ORC) Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome C IDA; IDA; IDA DNA replication preinitiation complex; nuclear origin of replication recognition complex; nuclear pre-replicative complex GO_0031261; GO_0005664; GO_0005656 2914 YHR119W SET1 Histone methyltransferase, subunit of the COMPASS (Set1C) complex Histone methyltransferase, subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3K4; Set1p-dependent H3K4 trimethylation recruits Nrd1p, allowing efficient termination of snoRNAs and cryptic unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway; modulates histone acetylation levels in promoter proximal regions to ensure efficient Nrd1p-dependent termination; required in transcriptional silencing near telomeres and at silent mating type loci; has a SET domain C IPI Set1C/COMPASS complex GO_0048188 2915 YHR120W MSH1 DNA-binding protein of the mitochondria DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis C IDA mitochondrion GO_0005739 2916 YHR121W LSM12 Protein of unknown function that may function in RNA processing Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress C HDA; IDA; HDA; IDA nucleus; cytoplasmic stress granule; cytoplasm; ribosome GO_0005634; GO_0010494; GO_0005737; GO_0005840 2917 YHR122W CIA2 Component of cytosolic iron-sulfur protein assembly (CIA) machinery Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 2918 YHR123W EPT1 sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication C HDA; HDA Golgi apparatus; endoplasmic reticulum GO_0005794; GO_0005783 2919 YHR124W NDT80 Meiosis-specific transcription factor Meiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) C IDA nuclear chromosome GO_0000228 2920 YHR125W YHR125W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2921 YHR126C ANS1 Putative GPI protein Putative GPI protein; transcription dependent upon Azf1p C HDA fungal-type vacuole GO_0000324 2922 YHR127W YHR127W Protein of unknown function Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis C IDA nucleus GO_0005634 2923 YHR128W FUR1 Uracil phosphoribosyltransferase Uracil phosphoribosyltransferase; synthesizes UMP from uracil; involved in the pyrimidine salvage pathway 2924 YHR129C ARP1 Actin-related protein of the dynactin complex Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin C IDA; IDA; IDA; IDA dynactin complex; cell cortex; spindle pole body; astral microtubule GO_0005869; GO_0005938; GO_0005816; GO_0000235 2925 YHR130C YHR130C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2926 YHR131C YHR131C Putative protein of unknown function Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 2927 YHR131W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YHR131C 2928 YHR132C ECM14 Putative metalloprotease with similarity to zinc carboxypeptidases Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly C HDA fungal-type vacuole GO_0000324 2929 YHR132W-A IGO2 Protein required for initiation of G0 program Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2930 YHR133C NSG1 Protein involved in regulation of sterol biosynthesis Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA endoplasmic reticulum; cytosol; nucleus; nuclear periphery GO_0005783; GO_0005829; GO_0005634; GO_0034399 2931 YHR134W WSS1 SUMO-ligase and SUMO-targeted metalloprotease Metalloprotease involved in DNA repair, removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; sumoylated protein localizing to the nuclear periphery of mother cells; localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; activated by DNA binding C IDA; IDA fungal-type vacuole; nuclear envelope GO_0000324; GO_0005635 2932 YHR135C YCK1 Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK1 has a paralog, YCK2, that arose from the whole genome duplication C HDA; IDA; HDA; HDA endoplasmic reticulum; plasma membrane; mitochondrion; cell periphery GO_0005783; GO_0005886; GO_0005739; GO_0071944 2933 YHR136C SPL2 Protein with similarity to cyclin-dependent kinase inhibitors Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 2934 YHR137C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene ARO9/YHR137W; identified by gene-trapping, expression analysis, and genome-wide homology searching 2935 YHR137W ARO9 Aromatic aminotransferase II Aromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2936 YHR138C YHR138C Protein of unknown function Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress 2937 YHR139C SPS100 Protein required for spore wall maturation Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin; SPS100 has a paralog, YGP1, that arose from the whole genome duplication 2938 YHR139C-A YHR139C-A Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2939 YHR140W YHR140W Putative integral membrane protein of unknown function Putative integral membrane protein of unknown function C HDA; HDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 2940 YHR141C RPL42B Ribosomal 60S subunit protein L42B Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 2941 YHR142W CHS7 Protein of unknown function Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress C HDA; HDA; HDA; HDA; IDA cellular bud neck; prospore membrane; cellular bud tip; endoplasmic reticulum; endoplasmic reticulum membrane GO_0005935; GO_0005628; GO_0005934; GO_0005783; GO_0005789 2942 YHR143W DSE2 Daughter cell-specific secreted protein with similarity to glucanases Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP C HDA; IDA; IDA fungal-type vacuole; fungal-type cell wall; extracellular region GO_0000324; GO_0009277; GO_0005576 2943 YHR143W-A RPC10 RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress C IDA; HDA; HDA; IDA; IDA; HDA DNA-directed RNA polymerase III complex; nucleolus; cytoplasm; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex; nucleus GO_0005666; GO_0005730; GO_0005737; GO_0005736; GO_0005665; GO_0005634 2944 YHR144C DCD1 Deoxycytidine monophosphate (dCMP) deaminase Deoxycytidine monophosphate (dCMP) deaminase; involved in dUMP and dTMP biosynthesis; expression is NOT cell cycle regulated C HDA cytoplasm GO_0005737 2945 YHR145C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2946 YHR146W CRP1 Protein that binds to cruciform DNA structures Protein that binds to cruciform DNA structures; CRP1 has a paralog, MDG1, that arose from the whole genome duplication 2947 YHR147C MRPL6 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 2948 YHR148W IMP3 Component of the SSU processome Component of the SSU processome; SSU processome is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA C IDA; IDA; HDA small-subunit processome; Mpp10 complex; 90S preribosome GO_0032040; GO_0034457; GO_0030686 2949 YHR149C SKG6 Integral membrane protein Integral membrane protein; localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p; SKG6 has a paralog, TOS2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA incipient cellular bud site; cell periphery; cellular bud neck; cellular bud tip; integral component of membrane GO_0000131; GO_0071944; GO_0005935; GO_0005934; GO_0016021 2950 YHR150W PEX28 Peroxisomal integral membrane peroxin Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p C HDA; IDA cytosol; peroxisomal membrane GO_0005829; GO_0005778 2951 YHR151C MTC6 Protein of unknown function Protein of unknown function; mtc6 is synthetically sick with cdc13-1 C HDA fungal-type vacuole GO_0000324 2952 YHR152W SPO12 Nucleolar protein of unknown function Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication C IDA; IDA nucleus; nucleolus GO_0005634; GO_0005730 2953 YHR153C SPO16 Meiosis-specific protein involved in synaptonemal complex assembly Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation C IDA condensed nuclear chromosome GO_0000794 2954 YHR154W RTT107 Protein implicated in Mms22-dependent DNA repair during S phase Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress C HDA; HDA; IDA cell periphery; nucleus; Cul8-RING ubiquitin ligase complex GO_0071944; GO_0005634; GO_0035361 2955 YHR155W LAM1 Putative sterol transfer protein Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication C IDA; IDA; HDA mitochondrion; cortical endoplasmic reticulum; cytosol GO_0005739; GO_0032541; GO_0005829 2956 YHR156C LIN1 Non-essential component of U5 snRNP Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication C IDA; IPI; IDA; IDA nucleus; U4/U6 x U5 tri-snRNP complex; chromatin; U5 snRNP GO_0005634; GO_0046540; GO_0000785; GO_0005682 2957 YHR157W REC104 Protein involved in early stages of meiotic recombination Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination C IDA condensed nuclear chromosome GO_0000794 2958 YHR158C KEL1 Protein required for proper cell fusion and cell morphology Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; KEL1 has a paralog, KEL2, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; IDA; IDA; HDA Kelch-containing formin regulatory complex; mating projection tip; cytoplasm; cellular bud tip; cell cortex; cellular bud neck; prospore membrane GO_1990615; GO_0043332; GO_0005737; GO_0005934; GO_0005938; GO_0005935; GO_0005628 2959 YHR159W TDA11 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele C HDA cytoplasm GO_0005737 2960 YHR160C PEX18 Peroxin Peroxin; required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p; PEX18 has a paralog, PEX21, that arose from the whole genome duplication C IDA; IDA peroxisome; cytosol GO_0005777; GO_0005829 2961 YHR161C YAP1801 Protein of the AP180 family, involved in clathrin cage assembly Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication C HDA; HDA cellular bud neck; prospore membrane GO_0005935; GO_0005628 2962 YHR162W MPC2 Highly conserved subunit of the mitochondrial pyruvate carrier (MPC) Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Mpc1p and either Mpc2p or Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; MPC2 has a paralog, FMP43, that arose from the whole genome duplication C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 2963 YHR163W SOL3 6-phosphogluconolactonase 6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 2964 YHR164C DNA2 Tripartite DNA replication factor Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress C IDA; IDA; IDA nucleus; cytoplasm; nuclear chromosome, telomeric region GO_0005634; GO_0005737; GO_0000784 2965 YHR165C PRP8 Component of U4/U6-U5 snRNP complex Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in its human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome. C IDA; IDA; HDA U4/U6 x U5 tri-snRNP complex; U5 snRNP; nucleus GO_0046540; GO_0005682; GO_0005634 2966 YHR165W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF PRP8/YHR165C 2967 YHR166C CDC23 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition C IDA anaphase-promoting complex GO_0005680 2968 YHR167W THP2 Subunit of the THO and TREX complexes Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance C IPI; IDA nucleoplasmic THO complex; THO complex part of transcription export complex GO_0000446; GO_0000445 2969 YHR168W MTG2 Putative GTPase Putative GTPase; member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly C IDA; IDA extrinsic component of membrane; mitochondrial inner membrane GO_0019898; GO_0005743 2970 YHR169W DBP8 ATPase, putative RNA helicase of the DEAD-box family ATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esf2p C IDA; HDA nucleolus; 90S preribosome GO_0005730; GO_0030686 2971 YHR170W NMD3 Protein involved in nuclear export of the large ribosomal subunit Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex C IDA; IDA; HDA cytosolic large ribosomal subunit; cytosol; preribosome, large subunit precursor GO_0022625; GO_0005829; GO_0030687 2972 YHR171W ATG7 Autophagy-related protein and dual specificity member of the E1 family Autophagy-related protein and dual specificity member of the E1 family; mediates the attachment of Atg12p to Atg5p and Atg8p to phosphatidylethanolamine which are required steps in autophagosome formation; E1 enzymes are also known as ubiquitin-activating enzymes; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner C IDA; IDA; IDA; HDA pre-autophagosomal structure; membrane; cytosol; mitochondrion GO_0000407; GO_0016020; GO_0005829; GO_0005739 2973 YHR172W SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque C IDA; IDA; IDA; HDA gamma-tubulin small complex, spindle pole body; outer plaque of spindle pole body; inner plaque of spindle pole body; spindle pole body GO_0000928; GO_0005824; GO_0005822; GO_0005816 2974 YHR173C YHR173C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels C HDA fungal-type vacuole GO_0000324 2975 YHR174W ENO2 Enolase II, a phosphopyruvate hydratase Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA; IDA; IDA fungal-type vacuole; plasma membrane; cytosol; mitochondrion; cytoplasmic side of plasma membrane; phosphopyruvate hydratase complex GO_0000324; GO_0005886; GO_0005829; GO_0005739; GO_0009898; GO_0000015 2976 YHR175W CTR2 Low-affinity copper transporter of the vacuolar membrane Putative low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation; regulated by nonsense-mediated mRNA decay pathway C IDA; HDA fungal-type vacuole membrane; endoplasmic reticulum GO_0000329; GO_0005783 2977 YHR175W-A YHR175W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 2978 YHR176W FMO1 Flavin-containing monooxygenase Flavin-containing monooxygenase; localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins C IDA; HDA endoplasmic reticulum membrane; cytosol GO_0005789; GO_0005829 2979 YHR177W ROF1 Putative transcription factor containing a WOPR domain Putative transcription factor containing a WOPR domain; binds DNA in vitro; similar to C. albicans Wor1p transcription factor that regulates white-opaque switching; overexpression causes a cell cycle delay or arrest 2980 YHR178W STB5 Transcription factor Transcription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro C IPI nucleus GO_0005634 2981 YHR179W OYE2 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN) Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 2982 YHR180C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YHR180W-A 2983 YHR180W YHR180W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 2984 YHR180W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YHR180C-B and long terminal repeat YHRCsigma3 2985 YHR181W SVP26 Integral membrane protein of the early Golgi apparatus and ER Integral membrane protein of the early Golgi apparatus and ER; involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment C IDA; IDA; HDA; IDA integral component of endoplasmic reticulum membrane; integral component of Golgi membrane; endoplasmic reticulum; ER to Golgi transport vesicle GO_0030176; GO_0030173; GO_0005783; GO_0030134 2986 YHR182C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF GND1/YHR183W 2987 YHR182W YHR182W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA cytoplasm; cellular bud tip GO_0005737; GO_0005934 2988 YHR183W GND1 6-phosphogluconate dehydrogenase (decarboxylating) 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 2989 YHR184W SSP1 Protein involved in the control of meiotic nuclear division Protein involved in the control of meiotic nuclear division; also involved in the coordination of meiosis with spore formation; transcription is induced midway through meiosis C IDA; IDA prospore membrane leading edge; prospore membrane GO_0070056; GO_0005628 2990 YHR185C PFS1 Sporulation protein required for prospore membrane formation Sporulation protein required for prospore membrane formation; required for prospore membrane formation at selected spindle poles; ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation 2991 YHR186C KOG1 Subunit of TORC1 Subunit of TORC1; TORC1 is a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors C IDA; IPI; HDA; IDA; IDA plasma membrane; TORC1 complex; mitochondrion; fungal-type vacuole membrane; cytoplasmic stress granule GO_0005886; GO_0031931; GO_0005739; GO_0000329; GO_0010494 2992 YHR187W IKI1 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin C IDA; IDA; IDA nucleus; Elongator holoenzyme complex; cytoplasm GO_0005634; GO_0033588; GO_0005737 2993 YHR188C GPI16 Subunit of the glycosylphosphatidylinositol transamidase complex Subunit of the glycosylphosphatidylinositol transamidase complex; transmembrane protein; adds GPIs to newly synthesized proteins; human PIG-Tp homolog C IDA; HDA GPI-anchor transamidase complex; endoplasmic reticulum GO_0042765; GO_0005783 2994 YHR189W PTH1 One of two mitochondrially-localized peptidyl-tRNA hydrolases One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2 C HDA mitochondrion GO_0005739 2995 YHR190W ERG9 Farnesyl-diphosphate farnesyl transferase (squalene synthase) Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway C IDA; HDA; HDA; HDA integral component of membrane; endoplasmic reticulum; mitochondrial outer membrane; mitochondrion GO_0016021; GO_0005783; GO_0005741; GO_0005739 2996 YHR191C CTF8 Subunit of a complex with Ctf18p Subunit of a complex with Ctf18p; shares some subunits with Replication Factor C; required for sister chromatid cohesion C IPI Ctf18 RFC-like complex GO_0031390 2997 YHR192W LNP1 Lunapark family member involved in ER network formation Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS C IDA; HDA; HDA endoplasmic reticulum tubular network; cytoplasm; endoplasmic reticulum GO_0071782; GO_0005737; GO_0005783 2998 YHR193C EGD2 Alpha subunit of the nascent polypeptide-associated complex (NAC) Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes C IDA nascent polypeptide-associated complex GO_0005854 2999 YHR193C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified ORF MDM31/YHR194W 3000 YHR194W MDM31 Mitochondrial protein that may have a role in phospholipid metabolism Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 C HDA; IDA nucleus; mitochondrial inner membrane GO_0005634; GO_0005743 3001 YHR195W NVJ1 Nuclear envelope protein Nuclear envelope protein; anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) C IDA; HDA; IDA; IDA nuclear envelope; nuclear periphery; integral component of membrane; nucleus-vacuole junction GO_0005635; GO_0034399; GO_0016021; GO_0071561 3002 YHR196W UTP9 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C IDA; IDA; IDA; HDA; IDA; HDA t-UTP complex; nucleolus; rDNA heterochromatin; nucleus; small-subunit processome; 90S preribosome GO_0034455; GO_0005730; GO_0033553; GO_0005634; GO_0032040; GO_0030686 3003 YHR197W RIX1 Component of the Rix1 complex and possibly pre-replicative complexes Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene C IDA; HDA; IDA; IDA nucleoplasm; nucleus; cytosol; Rix1 complex GO_0005654; GO_0005634; GO_0005829; GO_0097344 3004 YHR198C AIM18 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 3005 YHR199C AIM46 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 3006 YHR199C-A NBL1 Subunit of the conserved chromosomal passenger complex (CPC) Subunit of the conserved chromosomal passenger complex (CPC); complex regulates mitotic chromosome segregation; not required for the kinase activity of the complex; mediates the interaction of Sli15p and Bir1p; other complex members are Ipl1p, Sli15p, and Bir1p C IDA chromosome passenger complex GO_0032133 3007 YHR200W RPN10 Non-ATPase base subunit of the 19S RP of the 26S proteasome Non-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein C IPI; IMP proteasome regulatory particle, base subcomplex; proteasome complex GO_0008540; GO_0000502 3008 YHR201C PPX1 Exopolyphosphatase Exopolyphosphatase; hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix C IDA cytoplasm GO_0005737 3009 YHR202W YHR202W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization C HDA; IDA fungal-type vacuole; cytosol GO_0000324; GO_0005829 3010 YHR203C RPS4B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication C IDA; HDA cytosolic small ribosomal subunit; 90S preribosome GO_0022627; GO_0030686 3011 YHR204W MNL1 Alpha-1,2-specific exomannosidase of the endoplasmic reticulum Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation C IDA; HDA endoplasmic reticulum lumen; endoplasmic reticulum GO_0005788; GO_0005783 3012 YHR205W SCH9 AGC family protein kinase AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan C HDA; IDA; IDA; IDA cytoplasm; fungal-type vacuole membrane; nucleus; chromatin GO_0005737; GO_0000329; GO_0005634; GO_0000785 3013 YHR206W SKN7 Nuclear response regulator and transcription factor Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 3014 YHR207C SET5 Methyltransferase involved in methylation of histone H4 Lys5, -8, -12 Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus C IDA; HDA; HDA nuclear chromatin; cytoplasm; nucleus GO_0000790; GO_0005737; GO_0005634 3015 YHR208W BAT1 Mitochondrial branched-chain amino acid (BCAA) aminotransferase Mitochondrial branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA biosynthesis; homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase; BAT1 has a paralog, BAT2, that arose from the whole genome duplication C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 3016 YHR209W CRG1 S-AdoMet-dependent methyltransferase involved in lipid homeostasis S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin 3017 YHR210C YHR210C Putative aldose 1-epimerase superfamily protein Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions 3018 YHR211W FLO5 Lectin-like cell wall protein (flocculin) involved in flocculation Lectin-like cell wall protein (flocculin) involved in flocculation; binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; FLO5 has a paralog, FLO1, that arose from a segmental duplication C IDA; HDA fungal-type cell wall; cell periphery GO_0009277; GO_0071944 3019 YHR212C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YHR212C has a paralog, YAR060C, that arose from a segmental duplication 3020 YHR212W-A YHR212W-A Pseudogenic fragment with similarity to flocculins Pseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR212W-A has a paralog, YAR061W, that arose from a segmental duplication C HDA endoplasmic reticulum GO_0005783 3021 YHR213W YHR213W Pseudogenic fragment with similarity to flocculins Pseudogenic fragment with similarity to flocculins; YHR213W has a paralog, YAR062W, that arose from a segmental duplication 3022 YHR213W-A YHR213W-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3023 YHR213W-B YHR213W-B Pseudogenic fragment with similarity to flocculins Pseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR213W-B has a paralog, YAR064W, that arose from a segmental duplication 3024 YHR214C-B YHR214C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes; YHR214C-B has a paralog, YAR070C, that arose from a segmental duplication C IDA nucleus GO_0005634 3025 YHR214C-C YHR214C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 3026 YHR214C-D YHR214C-D Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR214C-D has a paralog, YAR069C, that arose from a segmental duplication 3027 YHR214C-E YHR214C-E Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3028 YHR214W YHR214W Putative protein of unknown function Putative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein; YHR214W has a paralog, YAR066W, that arose from a segmental duplication 3029 YHR214W-A Dubious open reading frame; induced by zinc deficiency; YHR214W-A has a paralog, YAR068W, that arose from a segmental duplication 3030 YHR215W PHO12 One of three repressible acid phosphatases One of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; pregulated by phosphate starvation; PHO12 has a paralog, PHO11, that arose from a segmental duplication C HDA fungal-type vacuole GO_0000324 3031 YHR216W IMD2 Inosine monophosphate dehydrogenase Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication C IDA; IDA cytoplasm; chromatin GO_0005737; GO_0000785 3032 YHR217C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; located in the telomeric region TEL08R 3033 YHR218W YHR218W Helicase-like protein encoded within the telomeric Y' element Helicase-like protein encoded within the telomeric Y' element 3034 YHR218W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely within telomere TEL08L 3035 YHR219C-A Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3036 YHR219W YHR219W Putative protein of unknown function with similarity to helicases Putative protein of unknown function with similarity to helicases; located in the telomere region on the right arm of chromosome VIII 3037 YIL001W YIL001W Putative protein of unknown function Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm C HDA cytoplasm GO_0005737 3038 YIL002C INP51 Phosphatidylinositol 4,5-bisphosphate 5-phosphatase Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth C IDA; IDA membrane; cytoplasm GO_0016020; GO_0005737 3039 YIL002W-A CMI7 Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 3040 YIL003W CFD1 Highly conserved iron-sulfur cluster binding protein Highly conserved iron-sulfur cluster binding protein; localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol C IDA; IDA cytoplasm; Nbp35-Cfd1 ATPase complex GO_0005737; GO_1904564 3041 YIL004C BET1 Type II membrane protein required for vesicular transport Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins C IDA; IDA; IDA; IDA SNARE complex; integral component of membrane; ER to Golgi transport vesicle; endoplasmic reticulum membrane GO_0031201; GO_0016021; GO_0030134; GO_0005789 3042 YIL005W EPS1 ER protein with chaperone and co-chaperone activity ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family C IDA endoplasmic reticulum membrane GO_0005789 3043 YIL006W YIA6 Mitochondrial NAD+ transporter Mitochondrial NAD+ transporter; involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs; YIA6 has a paralog, YEA6, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 3044 YIL007C NAS2 Evolutionarily conserved 19S regulatory particle assembly-chaperone Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA nucleus; cytosol; cytoplasm GO_0005634; GO_0005829; GO_0005737 3045 YIL008W URM1 Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response C IDA; HDA; HDA cytosol; cytoplasm; nucleus GO_0005829; GO_0005737; GO_0005634 3046 YIL009C-A EST3 Component of the telomerase holoenzyme Component of the telomerase holoenzyme; involved in telomere replication C IDA; HDA; IDA; IMP nucleus; cytosol; telomerase holoenzyme complex; chromosome, telomeric region GO_0005634; GO_0005829; GO_0005697; GO_0000781 3047 YIL009W FAA3 Long chain fatty acyl-CoA synthetase Long chain fatty acyl-CoA synthetase; activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 3048 YIL010W DOT5 Nuclear thiol peroxidase Nuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth C IDA nucleus GO_0005634 3049 YIL011W TIR3 Cell wall mannoprotein Cell wall mannoprotein; member of Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; TIR3 has a paralog, TIR2, that arose from the whole genome duplication C HDA; IDA; HDA fungal-type vacuole; fungal-type cell wall; cell periphery GO_0000324; GO_0009277; GO_0071944 3050 YIL012W YIL012W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3051 YIL013C PDR11 ATP-binding cassette (ABC) transporter ATP-binding cassette (ABC) transporter; multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth; PDR11 has a paralog, AUS1, that arose from the whole genome duplication C HDA cell periphery GO_0071944 3052 YIL014C-A YIL014C-A Putative protein of unknown function Putative protein of unknown function 3053 YIL014W MNT3 Alpha-1,3-mannosyltransferase Alpha-1,3-mannosyltransferase; adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation C HDA fungal-type vacuole GO_0000324 3054 YIL015W BAR1 Aspartyl protease Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest C IDA; HDA; IDA fungal-type cell wall; cell periphery; extracellular region GO_0009277; GO_0071944; GO_0005576 3055 YIL016W SNL1 Ribosome-associated protein Ribosome-associated protein; proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein C HDA; IDA; IDA; IDA mitochondrion; cytosolic ribosome; nuclear envelope; endoplasmic reticulum membrane GO_0005739; GO_0022626; GO_0005635; GO_0005789 3056 YIL017C VID28 GID Complex subunit, serves as adaptor for regulatory subunit Vid24p GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm C IDA; HDA; HDA GID complex; cytoplasm; nucleus GO_0034657; GO_0005737; GO_0005634 3057 YIL018W RPL2B Ribosomal 60S subunit protein L2B Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures C IDA cytosolic large ribosomal subunit GO_0022625 3058 YIL019W FAF1 Protein required for pre-rRNA processing Protein required for pre-rRNA processing; also required for 40S ribosomal subunit assembly C HDA; HDA; IDA nucleus; cytoplasm; nucleolus GO_0005634; GO_0005737; GO_0005730 3059 YIL020C HIS6 Enzyme that catalyzes the fourth step in the histidine pathway Enzyme that catalyzes the fourth step in the histidine pathway; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3060 YIL020C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps origin of replication ARS918, partially overlaps ORF RPB3/YIL021W 3061 YIL021C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene RPB3/YIL021W; identified by gene-trapping, expression analysis, and genome-wide homology searching 3062 YIL021W RPB3 RNA polymerase II third largest subunit B44 RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit C IDA; IDA nucleoplasm; DNA-directed RNA polymerase II, core complex GO_0005654; GO_0005665 3063 YIL022W TIM44 Essential component of the TIM23 complex Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane C HDA; IDA mitochondrion; presequence translocase-associated import motor GO_0005739; GO_0001405 3064 YIL023C YKE4 Zinc transporter Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family C HDA endoplasmic reticulum GO_0005783 3065 YIL024C YIL024C Putative protein of unknown function Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p 3066 YIL025C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3067 YIL026C IRR1 Subunit of the cohesin complex Subunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability C IDA; IDA; IDA; IDA nuclear mitotic cohesin complex; cytoplasm; nucleus; cytosol GO_0034990; GO_0005737; GO_0005634; GO_0005829 3068 YIL027C EMC5 Member of conserved ER transmembrane complex Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT C HDA; IDA endoplasmic reticulum; ER membrane protein complex GO_0005783; GO_0072546 3069 YIL028W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3070 YIL029C YIL029C Putative protein of unknown function Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has a paralog, YPR071W, that arose from a single-locus duplication 3071 YIL029W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SSM4/YIL030C 3072 YIL030C SSM4 Membrane-embedded ubiquitin-protein ligase Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation C IDA; IDA; IDA; HDA; IDA nuclear inner membrane; Doa10p ubiquitin ligase complex; nuclear envelope; endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005637; GO_0000837; GO_0005635; GO_0005783; GO_0030176 3073 YIL030W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF ULP2/YIL031W 3074 YIL031W ULP2 Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins; plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate C IDA nucleus GO_0005634 3075 YIL032C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3076 YIL033C BCY1 Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA) Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation C IDA; IDA; HDA; IDA nuclear chromatin; plasma membrane; cytoplasm; nucleus GO_0000790; GO_0005886; GO_0005737; GO_0005634 3077 YIL034C CAP2 Beta subunit of the capping protein heterodimer (Cap1p and Cap2p) Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress C IDA; HDA; IDA; IDA; HDA; IPI; IDA incipient cellular bud site; plasma membrane; cellular bud tip; F-actin capping protein complex; mating projection tip; actin filament; actin cortical patch GO_0000131; GO_0005886; GO_0005934; GO_0008290; GO_0043332; GO_0005884; GO_0030479 3078 YIL035C CKA1 Alpha catalytic subunit of casein kinase 2 (CK2) Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching C IDA; IDA UTP-C complex; protein kinase CK2 complex GO_0034456; GO_0005956 3079 YIL036W CST6 Basic leucine zipper (bZIP) transcription factor from ATF/CREB family Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 3080 YIL037C PRM2 Pheromone-regulated protein Pheromone-regulated protein; predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion 3081 YIL038C NOT3 Component of the CCR4-NOT core complex, involved in mRNA decapping Subunit of CCR4-NOT global transcriptional regulator; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers C IPI; IDA CCR4-NOT core complex; cytoplasm GO_0030015; GO_0005737 3082 YIL039W TED1 GPI-glycan remodelase Conserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) C HDA endoplasmic reticulum GO_0005783 3083 YIL040W APQ12 Nuclear envelope/ER integral membrane protein Protein required for nuclear envelope morphology; nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism C IDA; IDA; IDA endoplasmic reticulum; integral component of membrane; nuclear envelope GO_0005783; GO_0016021; GO_0005635 3084 YIL041W GVP36 BAR domain protein that localizes to early and late Golgi vesicles BAR domain protein that localizes to early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis C HDA; IDA; HDA cytoplasm; integral component of Golgi membrane; cytosol GO_0005737; GO_0030173; GO_0005829 3085 YIL042C PKP1 Mitochondrial protein kinase Mitochondrial protein kinase; involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p C HDA; IDA mitochondrion; mitochondrial pyruvate dehydrogenase complex GO_0005739; GO_0005967 3086 YIL043C CBR1 Cytochrome b reductase Microsomal cytochrome b reductase; not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia C HDA; HDA; HDA mitochondrion; mitochondrial outer membrane; nucleus GO_0005739; GO_0005741; GO_0005634 3087 YIL044C AGE2 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif C HDA; HDA cytosol; clathrin-coated vesicle GO_0005829; GO_0030136 3088 YIL045W PIG2 Putative type-1 protein phosphatase targeting subunit Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3089 YIL046W MET30 F-box protein containing five copies of the WD40 motif F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p C IDA; IDA; IDA SCF ubiquitin ligase complex; nuclear SCF ubiquitin ligase complex; nucleus GO_0019005; GO_0043224; GO_0005634 3090 YIL046W-A YIL046W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 3091 YIL047C SYG1 Plasma membrane protein of unknown function Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency C IDA; HDA; HDA plasma membrane; mitochondrion; fungal-type vacuole membrane GO_0005886; GO_0005739; GO_0000329 3092 YIL047C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SYG1/YIL047C 3093 YIL048W NEO1 Phospholipid translocase (flippase), role in phospholipid asymmetry o Putative aminophospholipid translocase (flippase); involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus C HDA; IDA; IDA; IDA COPI-coated vesicle; endosome; Golgi membrane; Golgi apparatus GO_0030137; GO_0005768; GO_0000139; GO_0005794 3094 YIL049W DFG10 Probable polyprenol reductase Probable polyprenol reductase; catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human ortholog SRD5A3 confer CDG (Congenital Disorders of Glycosylation) C HDA endoplasmic reticulum GO_0005783 3095 YIL050W PCL7 Pho85p cyclin of the Pho80p subfamily Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication C IPI; IDA cyclin-dependent protein kinase holoenzyme complex; nucleus GO_0000307; GO_0005634 3096 YIL051C MMF1 Mitochondrial protein required for transamination of isoleucine Mitochondrial protein required for transamination of isoleucine; but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; induction of expression in response to stress is mediated by a Hog1p-regulated antisense RNA and gene looping; interacts genetically with mitochondrial ribosomal protein genes; MMF1 has a paralog, HMF1, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 3097 YIL052C RPL34B Ribosomal 60S subunit protein L34B Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 3098 YIL053W GPP1 Constitutively expressed DL-glycerol-3-phosphate phosphatase Constitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication C IDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3099 YIL054W YIL054W Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels 3100 YIL055C YIL055C Putative protein of unknown function Putative protein of unknown function C HDA mitochondrion GO_0005739 3101 YIL056W VHR1 Transcriptional activator Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3102 YIL057C RGI2 Protein of unknown function Protein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3103 YIL058W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3104 YIL059C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W 3105 YIL060W YIL060W Mitochondrial protein of unknown function Mitochondrial protein of unknown function; required for respiratory growth; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene C IDA mitochondrion GO_0005739 3106 YIL061C SNP1 Component of U1 snRNP required for mRNA splicing via spliceosome Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress C IPI; IDA; IDA commitment complex; U1 snRNP; U2-type prespliceosome GO_0000243; GO_0005685; GO_0071004 3107 YIL062C ARC15 Subunit of the ARP2/3 complex Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity C IDA; IDA Arp2/3 protein complex; extrinsic component of mitochondrial outer membrane GO_0005885; GO_0031315 3108 YIL063C YRB2 Protein of unknown function Protein of unknown function; involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; relocalizes to the cytosol in response to hypoxia; not essential for viability C IDA; IDA; IDA nucleus; nuclear pore; cytosol GO_0005634; GO_0005643; GO_0005829 3109 YIL064W EFM4 Lysine methyltransferase Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport C HDA cytoplasm GO_0005737 3110 YIL065C FIS1 Protein involved in mitochondrial fission and peroxisome abundance Protein involved in mitochondrial fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation C IDA; HDA; IDA mitochondrial outer membrane; mitochondrion; peroxisome GO_0005741; GO_0005739; GO_0005777 3111 YIL066C RNR3 Minor isoform of large subunit of ribonucleotide-diphosphate reductase Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication C IDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 3112 YIL066W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YIL067C 3113 YIL067C YIL067C Uncharacterized protein of unknown function Uncharacterized protein of unknown function C HDA fungal-type vacuole GO_0000324 3114 YIL068C SEC6 Essential 88kDa subunit of the exocyst complex Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p C HDA; IDA; IDA; IDA; HDA mating projection tip; cellular bud tip; exocyst; cellular bud neck; prospore membrane GO_0043332; GO_0005934; GO_0000145; GO_0005935; GO_0005628 3115 YIL068W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SEC6/YIL068C 3116 YIL069C RPS24B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication 3117 YIL070C MAM33 Specific translational activator for the mitochondrial COX1 mRNA Acidic protein of the mitochondrial matrix; involved in oxidative phosphorylation; related to the human complement receptor gC1q-R C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 3118 YIL071C PCI8 Possible shared subunit of Cop9 signalosome (CSN) and eIF3 Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain C IDA COP9 signalosome GO_0008180 3119 YIL071W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF PCI8/YIL071C 3120 YIL072W HOP1 Meiosis-specific protein required for chromosome synapsis Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein-DNA filaments C HDA; IPI; IDA nucleus; lateral element; condensed nuclear chromosome GO_0005634; GO_0000800; GO_0000794 3121 YIL073C SPO22 Meiosis-specific protein essential for chromosome synapsis Meiosis-specific protein essential for chromosome synapsis; involved in completion of nuclear divisions during meiosis; induced early in meiosis C IDA; HDA condensed nuclear chromosome; nucleus GO_0000794; GO_0005634 3122 YIL074C SER33 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3123 YIL075C RPN2 Subunit of the 26S proteasome Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA proteasome regulatory particle, base subcomplex; proteasome storage granule; nucleus GO_0008540; GO_0034515; GO_0005634 3124 YIL076W SEC28 Epsilon-COP subunit of the coatomer Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress C IMP; IDA COPI vesicle coat; endosome GO_0030126; GO_0005768 3125 YIL077C RCI37 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) C HDA mitochondrion GO_0005739 3126 YIL078W THS1 Threonyl-tRNA synthetase Threonyl-tRNA synthetase; essential cytoplasmic protein C IMP; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 3127 YIL079C AIR1 Zinc knuckle protein Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome C IDA; IDA TRAMP complex; nucleolus GO_0031499; GO_0005730 3128 YIL082W Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 3129 YIL082W-A YIL082W-A Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 3130 YIL083C CAB2 Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) C HDA; HDA; IDA cytoplasm; nucleus; CoA-synthesizing protein complex GO_0005737; GO_0005634; GO_1990143 3131 YIL084C SDS3 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes C IDA; IDA; IDA; HDA Rpd3L complex; cytosol; nucleus; Rpd3L-Expanded complex GO_0033698; GO_0005829; GO_0005634; GO_0070210 3132 YIL085C KTR7 Putative mannosyltransferase involved in protein glycosylation Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication 3133 YIL086C YIL086C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3134 YIL087C AIM19 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth C HDA mitochondrion GO_0005739 3135 YIL088C AVT7 Putative transporter Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters C HDA; IDA fungal-type vacuole; plasma membrane GO_0000324; GO_0005886 3136 YIL089W YIL089W Protein of unknown function found in the ER and vacuole lumen Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking C HDA; IDA; IDA; HDA peroxisome; fungal-type vacuole lumen; endoplasmic reticulum; fungal-type vacuole GO_0005777; GO_0000328; GO_0005783; GO_0000324 3137 YIL090W ICE2 Integral ER membrane protein with type-III transmembrane domains Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells C IDA; IDA; HDA; IDA perinuclear endoplasmic reticulum; integral component of endoplasmic reticulum membrane; endoplasmic reticulum; cortical endoplasmic reticulum GO_0097038; GO_0030176; GO_0005783; GO_0032541 3138 YIL091C UTP25 Nucleolar protein Nucleolar protein; required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis C IDA; HDA; HDA small-subunit processome; nucleolus; nucleus GO_0032040; GO_0005730; GO_0005634 3139 YIL092W YIL092W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3140 YIL093C RSM25 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 3141 YIL094C LYS12 Homo-isocitrate dehydrogenase Homo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate C HDA mitochondrion GO_0005739 3142 YIL095W PRK1 Protein serine/threonine kinase Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication C IDA; HDA actin cortical patch; mating projection tip GO_0030479; GO_0043332 3143 YIL096C BMT5 Methyltransferase required for m3U2634 methylation of the 25S rRNA Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis C IDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 3144 YIL097W FYV10 Subunit of GID complex Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin C IDA; HDA; HDA GID complex; cytoplasm; nucleus GO_0034657; GO_0005737; GO_0005634 3145 YIL098C FMC1 Mitochondrial matrix protein Mitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 3146 YIL099W SGA1 Intracellular sporulation-specific glucoamylase Intracellular sporulation-specific glucoamylase; involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation C HDA; HDA fungal-type vacuole; prospore membrane GO_0000324; GO_0005628 3147 YIL100C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3148 YIL100W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A 3149 YIL101C XBP1 Transcriptional repressor Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3150 YIL102C YIL102C Putative protein of unknown function Putative protein of unknown function 3151 YIL102C-A YIL102C-A Putative protein of unknown function Putative protein of unknown function; identified based on comparisons of the genome sequences of six Saccharomyces species C HDA endoplasmic reticulum GO_0005783 3152 YIL103W DPH1 Protein required for synthesis of diphthamide Protein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p C HDA cytoplasm GO_0005737 3153 YIL104C SHQ1 Chaperone protein Chaperone protein; required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing; functions as an RNA mimic; forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; nucleus; nucleoplasm GO_0005829; GO_0005634; GO_0005654 3154 YIL105C SLM1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication C IDA; HDA; IDA; IDA; HDA membrane raft; mitochondrion; eisosome; plasma membrane; cytoplasm GO_0045121; GO_0005739; GO_0032126; GO_0005886; GO_0005737 3155 YIL105W-A YIL105W-A Protein of unknown function Protein of unknown function; completely overlaps the verified gene SLM1; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; mRNA identified as translated by ribosome profiling data 3156 YIL106W MOB1 Component of the mitotic exit network Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress C HDA; IDA; IDA; HDA nuclear periphery; cellular bud neck; spindle pole body; cytoplasm GO_0034399; GO_0005935; GO_0005816; GO_0005737 3157 YIL107C PFK26 6-phosphofructo-2-kinase 6-phosphofructo-2-kinase; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A 3158 YIL108W YIL108W Putative metalloendopeptidase Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress C HDA cytoplasm GO_0005737 3159 YIL109C SEC24 Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication C HDA; IDA fungal-type vacuole membrane; COPII vesicle coat GO_0000329; GO_0030127 3160 YIL110W HPM1 AdoMet-dependent methyltransferase AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3161 YIL111W COX5B Subunit Vb of cytochrome c oxidase Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication C IDA; HDA mitochondrial respiratory chain complex IV; mitochondrion GO_0005751; GO_0005739 3162 YIL112W HOS4 Subunit of the Set3 complex Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate C IDA Set3 complex GO_0034967 3163 YIL113W SDP1 Stress-inducible dual-specificity MAP kinase phosphatase Stress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 3164 YIL114C POR2 Putative mitochondrial porin (voltage-dependent anion channel) Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 3165 YIL115C NUP159 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) C IDA; IDA; IDA nuclear pore cytoplasmic filaments; nuclear pore; nuclear pore central transport channel GO_0044614; GO_0005643; GO_0044613 3166 YIL115W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUP159/YIL115C 3167 YIL116W HIS5 Histidinol-phosphate aminotransferase Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts C HDA cytoplasm GO_0005737 3168 YIL117C PRM5 Pheromone-regulated protein, predicted to have 1 transmembrane segment Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication 3169 YIL118W RHO3 Non-essential small GTPase of the Rho/Rac family of Ras-like proteins Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p C IDA; HDA; IDA cellular bud; plasma membrane; cytosol GO_0005933; GO_0005886; GO_0005829 3170 YIL119C RPI1 Transcription factor, allelic differences between S288C and Sigma1278b Transcription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF C IDA nucleus GO_0005634 3171 YIL120W QDR1 Multidrug transporter of the major facilitator superfamily Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in spore wall assembly; sequence similarity to DTR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 3172 YIL121W QDR2 Plasma membrane transporter of the major facilitator superfamily Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper C IDA; HDA integral component of plasma membrane; cell periphery GO_0005887; GO_0071944 3173 YIL122W POG1 DNA-binding transcriptional activator Nuclear chromatin-associated protein of unknown function; may have a role in cell cycle regulation; overexpression promotes recovery from pheromone induced arrest and suppresses the stress sensitivity caused by a mutation in the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; phsosphoylated form may be ubiquitinated by Dma2p; potential Cdc28p substrate; SBF regulated C IDA; HDA nuclear chromatin; nucleus GO_0000790; GO_0005634 3174 YIL123W SIM1 Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p); may participate in DNA replication; promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated; SIM1 has a paralog, SUN4, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; fungal-type cell wall GO_0005783; GO_0009277 3175 YIL124W AYR1 Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase; NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase involved in phosphatidic acid biosynthesis; lipid droplet triacylglycerol lipase involved in the mobilization of non-polar lipids; found in lipid particles, the endoplasmic reticulum and the mitochondrial outer membrane; required for spore germination; role in cell wall biosynthesis; capable of metabolizing steroid hormones; oleic acid inducible C HDA; IDA; HDA; HDA; HDA endoplasmic reticulum; lipid particle; mitochondrion; cytoplasm; mitochondrial outer membrane GO_0005783; GO_0005811; GO_0005739; GO_0005737; GO_0005741 3176 YIL125W KGD1 Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA C IDA; HDA; IDA mitochondrial oxoglutarate dehydrogenase complex; mitochondrion; mitochondrial nucleoid GO_0009353; GO_0005739; GO_0042645 3177 YIL126W STH1 ATPase component of the RSC chromatin remodeling complex ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p C IDA; IDA RSC complex; nucleus GO_0016586; GO_0005634 3178 YIL127C RRT14 Putative protein of unknown function Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis C HDA nucleolus GO_0005730 3179 YIL128W MET18 Component of cytosolic iron-sulfur protein assembly (CIA) machinery Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 C HDA cytoplasm GO_0005737 3180 YIL129C TAO3 Component of the RAM signaling network Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p C IDA; IDA; IDA; HDA cellular bud; mating projection tip; incipient cellular bud site; mitochondrion GO_0005933; GO_0043332; GO_0000131; GO_0005739 3181 YIL130W ASG1 Zinc cluster protein proposed to be a transcriptional regulator Zinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance C HDA nucleus GO_0005634 3182 YIL131C FKH1 Forkhead family transcription factor Forkhead family transcription factor; minor role in expression of G2/M phase genes; negatively regulates transcription elongation; positive role in chromatin silencing at HML, HMR; facilitates clustering and activation of early-firing replication origins; binds to recombination enhancer near HML, regulates donor preference during mating-type switching; relocalizes to cytosol in response to hypoxia; FKH1 has a paralog, FKH2, that arose from the whole genome duplication C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 3183 YIL132C CSM2 Component of Shu complex (aka PCSS complex) Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair,; Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis C IMP; HDA; IPI; HDA site of double-strand break; nucleus; Shu complex; cytoplasm GO_0035861; GO_0005634; GO_0097196; GO_0005737 3184 YIL133C RPL16A Ribosomal 60S subunit protein L16A Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA cytosolic large ribosomal subunit GO_0022625 3185 YIL134C-A YIL134C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 3186 YIL134W FLX1 Mitochondrial flavin adenine dinucleotide transporter Protein required for transport of flavin adenine dinucleotide (FAD); a synthesis product of riboflavin, across the mitochondrial membrane C HDA mitochondrion GO_0005739 3187 YIL135C VHS2 Regulator of septin dynamics Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3188 YIL136W OM45 Mitochondrial outer membrane protein of unknown function Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA mitochondrion; mitochondrial outer membrane; integral component of mitochondrial outer membrane GO_0005739; GO_0005741; GO_0031307 3189 YIL137C TMA108 Ribosome-associated, nascent chain binding factor Ribosome-associated protein that is involved in ribosome biogenesis; putative metalloprotease C IDA; IDA; HDA ribosome; nascent polypeptide-associated complex; cytoplasm GO_0005840; GO_0005854; GO_0005737 3190 YIL138C TPM2 Minor isoform of tropomyosin Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication C IDA; IDA actin filament bundle; cellular bud neck contractile ring GO_0032432; GO_0000142 3191 YIL139C REV7 Accessory subunit of DNA polymerase zeta Accessory subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev3p, Pol31p and Pol32p C IDA; IDA; IDA zeta DNA polymerase complex; nuclear chromatin; mitochondrion GO_0016035; GO_0000790; GO_0005739 3192 YIL140W AXL2 Integral plasma membrane protein Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate C IDA; IDA; HDA; IDA; IDA; IDA cellular bud neck septin ring; cell division site; fungal-type vacuole; cellular bud neck; incipient cellular bud site; integral component of plasma membrane GO_0000144; GO_0032153; GO_0000324; GO_0005935; GO_0000131; GO_0005887 3193 YIL141W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3194 YIL142C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF CCT2/YIL142W 3195 YIL142W CCT2 Subunit beta of the cytosolic chaperonin Cct ring complex Subunit beta of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo C IDA; HDA chaperonin-containing T-complex; cytoplasm GO_0005832; GO_0005737 3196 YIL143C SSL2 Component of RNA polymerase transcription factor TFIIH holoenzyme Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to the cytosol in response to hypoxia; homolog of human ERCC3 C IDA; IDA; IDA; IDA; IDA; IDA holo TFIIH complex; nucleus; cytosol; core TFIIH complex; transcriptional preinitiation complex; nucleotide-excision repair factor 3 complex GO_0005675; GO_0005634; GO_0005829; GO_0000439; GO_0097550; GO_0000112 3197 YIL144W NDC80 Component of the kinetochore-associated Ndc80 complex Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation C IDA; IPI; IPI condensed nuclear chromosome, centromeric region; Ndc80 complex; condensed nuclear chromosome kinetochore GO_0000780; GO_0031262; GO_0000778 3198 YIL145C PAN6 Pantothenate synthase Pantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3199 YIL146C ATG32 Mitochondrial outer membrane protein required to initiate mitophagy Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane; regulates mitophagy and ethanol production during alcoholic fermentation C IDA integral component of mitochondrial outer membrane GO_0031307 3200 YIL147C SLN1 Transmembrane histidine phosphotransfer kinase and osmosensor Transmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators C HDA; IDA; IDA cell periphery; integral component of plasma membrane; plasma membrane GO_0071944; GO_0005887; GO_0005886 3201 YIL148W RPL40A Ubiquitin-ribosomal 60S subunit protein L40A fusion protein Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; IDA nucleus; cytoplasm; cytosolic large ribosomal subunit GO_0005634; GO_0005737; GO_0022625 3202 YIL149C MLP2 Myosin-like protein associated with the nuclear envelope Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IPI; HDA nuclear pore nuclear basket; spindle pole body; nucleoplasm; nuclear envelope; ribonucleoprotein complex; mitochondrion GO_0044615; GO_0005816; GO_0005654; GO_0005635; GO_1990904; GO_0005739 3203 YIL150C MCM10 Essential chromatin-associated protein Essential chromatin-associated protein; involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins; required to stabilize the catalytic subunit of DNA polymerase-alpha C IDA; IMP; IDA nucleus; replication fork; replication fork protection complex GO_0005634; GO_0005657; GO_0031298 3204 YIL151C ESL1 hEST1A/B (SMG5/6)-like protein Protein of unknown function; predicted to contain a PINc domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication 3205 YIL152W YIL152W Putative protein of unknown function Putative protein of unknown function 3206 YIL153W RRD1 Peptidyl-prolyl cis/trans-isomerase Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; IDA nucleus; cytoplasm; nuclear chromatin GO_0005634; GO_0005737; GO_0000790 3207 YIL154C IMP2' Transcriptional activator involved in maintenance of ion homeostasis Transcriptional activator involved in maintenance of ion homeostasis; also involved in protection against DNA damage caused by bleomycin and other oxidants; contains a C-terminal leucine-rich repeat C HDA cytoplasm GO_0005737 3208 YIL155C GUT2 Mitochondrial glycerol-3-phosphate dehydrogenase Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner C IDA; HDA integral component of mitochondrial outer membrane; mitochondrion GO_0031307; GO_0005739 3209 YIL156W UBP7 Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP7 has a paralog, UBP11, that arose from the whole genome duplication 3210 YIL156W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF COA1/YIL157C 3211 YIL156W-B MCO8 Putative protein of unknown function Putative protein of unknown function; originally identified based on homology to Ashbya gossypii and other related yeasts C HDA fungal-type vacuole GO_0000324 3212 YIL157C COA1 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly C HDA; IDA; IDA mitochondrion; integral component of mitochondrial inner membrane; integral component of mitochondrial membrane GO_0005739; GO_0031305; GO_0032592 3213 YIL158W AIM20 Protein of unknown function Putative protein of unknown function; overexpression causes cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress; AIM20 has a paralog, SKG1, that arose from the whole genome duplication C HDA; HDA; HDA nucleus; cytoplasm; fungal-type vacuole GO_0005634; GO_0005737; GO_0000324 3214 YIL159W BNR1 Formin Formin; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 C IDA; IDA cell division site; cellular bud neck GO_0032153; GO_0005935 3215 YIL160C POT1 3-ketoacyl-CoA thiolase with broad chain length specificity 3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids C IDA; IDA; IDA peroxisome; peroxisomal matrix; mitochondrial intermembrane space GO_0005777; GO_0005782; GO_0005758 3216 YIL161W YIL161W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene C HDA cytoplasm GO_0005737 3217 YIL162W SUC2 Invertase Invertase; sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively C HDA; HDA; IDA; IDA; HDA mitochondrion; cell periphery; extracellular region; cytoplasm; fungal-type vacuole GO_0005739; GO_0071944; GO_0005576; GO_0005737; GO_0000324 3218 YIL163C YIL163C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data 3219 YIL164C NIT1 Nitrilase Nitrilase; member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene 3220 YIL165C YIL165C Putative protein of unknown function Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene 3221 YIL166C SOA1 Sulfonate and inorganic sulfur transporter Putative protein with similarity to allantoate permease; similar to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene C HDA; HDA cell periphery; fungal-type vacuole GO_0071944; GO_0000324 3222 YIL169C CSS1 Protein of unknown function, secreted when constitutively expressed Putative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; YIL169C is a non-essential gene C HDA; HDA cell periphery; extracellular region GO_0071944; GO_0005576 3223 YIL171W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF IMA3/YIL172C 3224 YIL172C IMA3 Alpha-glucosidase Alpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima3p overexpression allows the ima1 null mutant to grow on isomaltose; lower activitiy and thermostability in vitro than Ima2p despite sequence difference of only 3 amino acids; cleaves alpha-1,3 linkage of nigerose and turanose, but not alpha-1,5 of leucrose; identical to IMA4 C HDA cytoplasm GO_0005737 3225 YIL173W VTH1 Putative membrane glycoprotein Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting C HDA; HDA endosome; fungal-type vacuole GO_0005768; GO_0000324 3226 YIL176C PAU14 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau1p 3227 YIL177C YIL177C Putative Y' element ATP-dependent helicase Putative Y' element ATP-dependent helicase 3228 YIL177W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YIL177C 3229 YIR001C SGN1 Cytoplasmic RNA-binding protein Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation C IDA cytoplasm GO_0005737 3230 YIR002C MPH1 3'-5' DNA helicase involved in error-free bypass of DNA lesions 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA in error-free bypass pathway, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; similar to FANCM human Fanconi anemia complementation group protein that with MHF complex is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases C IDA; IDA chromosome, telomeric region; nucleus GO_0000781; GO_0005634 3231 YIR003W AIM21 Subunit of a complex that associates with actin filaments Protein of unknown function; involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton C IDA; HDA; HDA; HDA actin cortical patch; mating projection tip; ribosome; actin cytoskeleton GO_0030479; GO_0043332; GO_0005840; GO_0015629 3232 YIR004W DJP1 Cytosolic J-domain-containing protein Cytosolic J-domain-containing protein; required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ C HDA; HDA; HDA; IDA cell periphery; cellular bud; endoplasmic reticulum; cytosol GO_0071944; GO_0005933; GO_0005783; GO_0005829 3233 YIR005W IST3 Component of the U2 snRNP Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids C IDA; IDA RES complex; U2 snRNP GO_0070274; GO_0005686 3234 YIR006C PAN1 Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease C IDA; IDA; IDA; HDA; HDA; HDA nucleus; cytoplasm; actin cortical patch; cellular bud neck; mating projection tip; plasma membrane GO_0005634; GO_0005737; GO_0030479; GO_0005935; GO_0043332; GO_0005886 3235 YIR007W EGH1 Steryl-beta-glucosidase with broad specificity for aglycones Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene C HDA; IDA; HDA cytoplasm; cytosol; fungal-type vacuole membrane GO_0005737; GO_0005829; GO_0000329 3236 YIR008C PRI1 Subunit of DNA primase Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair C IDA; IDA alpha DNA polymerase:primase complex; nuclear replication fork GO_0005658; GO_0043596 3237 YIR009W MSL1 U2B component of U2 snRNP U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members C IDA U2-type prespliceosome GO_0071004 3238 YIR010W DSN1 Essential component of the MIND kinetochore complex Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation of Dsn1p promotes interaction between outer and inner kinetochore proteins; N-terminal end interacts with monopolin subunit Csm1p and is essential for meiotic but not mitotic chromosome segregation; important for chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND); modified by sumoylation C IDA; IDA; IDA nuclear MIS12/MIND complex; spindle pole; kinetochore GO_0000818; GO_0000922; GO_0000776 3239 YIR011C STS1 Protein required for localizing proteasomes to the nucleus Protein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation C IDA nucleus GO_0005634 3240 YIR012W SQT1 Specific chaperone for ribosomal protein Rpl10p Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA; IDA preribosome, large subunit precursor; cytosol; cytoplasm; cytosolic ribosome GO_0030687; GO_0005829; GO_0005737; GO_0022626 3241 YIR013C GAT4 Protein containing GATA family zinc finger motifs Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT3, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants C HDA nucleus GO_0005634 3242 YIR014W VLD1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene C HDA; HDA fungal-type vacuole; endoplasmic reticulum GO_0000324; GO_0005783 3243 YIR015W RPR2 Subunit of nuclear RNase P Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress C IDA nucleolar ribonuclease P complex GO_0005655 3244 YIR016W YIR016W Putative protein of unknown function Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication 3245 YIR017C MET28 bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism C IDA; HDA Cbf1-Met4-Met28 complex; nucleus GO_0089713; GO_0005634 3246 YIR017W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3247 YIR018C-A YIR018C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 3248 YIR018W YAP5 Basic leucine zipper (bZIP) iron-sensing transcription factor Basic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication C IDA; IDA nuclear chromatin; nucleus GO_0000790; GO_0005634 3249 YIR019C FLO11 GPI-anchored cell surface glycoprotein (flocculin) GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain; a portion is cleaved and shed from cells, and free extracellular Flo11p contributes to the surface properties of cells C HDA; IDA; IDA; IDA fungal-type vacuole; cellular bud neck; plasma membrane; extracellular region GO_0000324; GO_0005935; GO_0005886; GO_0005576 3250 YIR020C YIR020C Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data 3251 YIR020C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF MRS1 3252 YIR020W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3253 YIR021W MRS1 Splicing protein Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication C IDA; HDA ribonucleoprotein complex; mitochondrion GO_1990904; GO_0005739 3254 YIR021W-A YIR021W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 3255 YIR022W SEC11 18kDa catalytic subunit of the Signal Peptidase Complex (SPC) 18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p C IDA; IDA endoplasmic reticulum; signal peptidase complex GO_0005783; GO_0005787 3256 YIR023C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF DAL81/YIR023W 3257 YIR023W DAL81 Positive regulator of genes in multiple nitrogen degradation pathways Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism 3258 YIR024C INA22 F1F0 ATP synthase peripheral stalk assembly factor F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect C IDA; HDA; IDA intrinsic component of mitochondrial inner membrane; mitochondrion; INA complex GO_0031304; GO_0005739; GO_1990524 3259 YIR025W MND2 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase C IDA; IDA nucleus; anaphase-promoting complex GO_0005634; GO_0005680 3260 YIR026C YVH1 Dual specificity protein phosphatase Protein phosphatase; involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases C HDA; HDA; IDA; IDA cytoplasm; preribosome, large subunit precursor; nucleus; cytoplasmic stress granule GO_0005737; GO_0030687; GO_0005634; GO_0010494 3261 YIR027C DAL1 Allantoinase Allantoinase; converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression 3262 YIR028W DAL4 Allantoin permease Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation C HDA fungal-type vacuole GO_0000324 3263 YIR029W DAL2 Allantoicase Allantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation 3264 YIR030C DCG1 Protein of unknown function Protein of unknown function; expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain 3265 YIR030W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF DAL7/YIR031C 3266 YIR031C DAL7 Malate synthase Malate synthase; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA; recycles glyoxylate generated during allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation C IDA; HDA peroxisome; cytosol GO_0005777; GO_0005829 3267 YIR032C DAL3 Ureidoglycolate lyase Ureidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to ureidoglycolate hydrolase but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide 3268 YIR033W MGA2 ER membrane protein involved in regulation of OLE1 transcription ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication C IDA; HDA; IDA nucleus; endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005634; GO_0005783; GO_0030176 3269 YIR034C LYS1 Saccharopine dehydrogenase (NAD+, L-lysine-forming) Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity C HDA cytoplasm GO_0005737 3270 YIR035C YIR035C Putative cytoplasmic short-chain dehydrogenase/reductase Putative cytoplasmic short-chain dehydrogenase/reductase C HDA cytoplasm GO_0005737 3271 YIR036C IRC24 Putative benzil reductase Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci C HDA cytoplasm GO_0005737 3272 YIR036W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF IRC24/YIR036C 3273 YIR037W HYR1 Thiol peroxidase Thiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication C IPI; IDA; HDA; IDA; HDA intracellular; peroxisomal matrix; mitochondrion; mitochondrial intermembrane space; cytosol GO_0005622; GO_0005782; GO_0005739; GO_0005758; GO_0005829 3274 YIR038C GTT1 ER associated glutathione S-transferase ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p C HDA; HDA; HDA; HDA; HDA plasma membrane; cell periphery; mitochondrion; mitochondrial outer membrane; endoplasmic reticulum GO_0005886; GO_0071944; GO_0005739; GO_0005741; GO_0005783 3275 YIR039C YPS6 Putative GPI-anchored aspartic protease Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance C IDA fungal-type cell wall GO_0009277 3276 YIR040C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3277 YIR041W PAU15 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions C HDA fungal-type vacuole GO_0000324 3278 YIR042C YIR042C Putative protein of unknown function Putative protein of unknown function; YIR042C is a non-essential gene 3279 YJL001W PRE3 Beta 1 subunit of the 20S proteasome Beta 1 subunit of the 20S proteasome; responsible for cleavage after acidic residues in peptides C IDA; IDA proteasome storage granule; proteasome core complex, beta-subunit complex GO_0034515; GO_0019774 3280 YJL002C OST1 Alpha subunit of the oligosaccharyltransferase complex of the ER lumen Alpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins C IPI; HDA oligosaccharyltransferase complex; endoplasmic reticulum GO_0008250; GO_0005783 3281 YJL003W COX16 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; required for assembly of cytochrome c oxidase C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 3282 YJL004C SYS1 Integral membrane protein of the Golgi Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation C IDA; IDA integral component of Golgi membrane; trans-Golgi network GO_0030173; GO_0005802 3283 YJL005W CYR1 Adenylate cyclase Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation C IDA; IDA; IDA; HDA endoplasmic reticulum membrane; plasma membrane; mitochondrion; cytosol GO_0005789; GO_0005886; GO_0005739; GO_0005829 3284 YJL006C CTK2 Beta subunit of C-terminal domain kinase I (CTDK-I) Beta subunit of C-terminal domain kinase I (CTDK-I); which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA cytosol; CTDK-1 complex; nucleus; nucleoplasm; nucleolus GO_0005829; GO_0070692; GO_0005634; GO_0005654; GO_0005730 3285 YJL007C YJL007C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3286 YJL008C CCT8 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo C IPI; HDA chaperonin-containing T-complex; cytoplasm GO_0005832; GO_0005737 3287 YJL009W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex 3288 YJL010C NOP9 Essential subunit of U3-containing 90S preribosome Essential subunit of U3-containing 90S preribosome; involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain C IDA; HDA; IDA; IDA nucleolus; nucleus; preribosome, small subunit precursor; 90S preribosome GO_0005730; GO_0005634; GO_0030688; GO_0030686 3289 YJL011C RPC17 RNA polymerase III subunit C17 RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA DNA-directed RNA polymerase III complex; cytosol; nucleus GO_0005666; GO_0005829; GO_0005634 3290 YJL012C VTC4 Vacuolar membrane polyphosphate polymerase Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress C IDA; HDA; HDA; IDA; IPI intrinsic component of vacuolar membrane; cell periphery; fungal-type vacuole membrane; endoplasmic reticulum; vacuolar transporter chaperone complex GO_0031310; GO_0071944; GO_0000329; GO_0005783; GO_0033254 3291 YJL013C MAD3 Subunit of spindle-assembly checkpoint complex Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication C IPI mitotic checkpoint complex GO_0033597 3292 YJL014W CCT3 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; capable of binding Q/N rich proteins and mediating their folding C IDA chaperonin-containing T-complex GO_0005832 3293 YJL015C Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W 3294 YJL016W TPH3 Putative protein of unknown function Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species C HDA cytoplasm GO_0005737 3295 YJL019W MPS3 Nuclear envelope protein Nuclear envelope protein; required for SPB insertion, initiation of SPB duplication and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology) C IDA; HDA; IDA; IDA; IDA; IDA nuclear envelope; nuclear periphery; nuclear chromosome, telomeric region; spindle pole body; half bridge of spindle pole body; integral component of membrane GO_0005635; GO_0034399; GO_0000784; GO_0005816; GO_0005825; GO_0016021 3296 YJL020C BBC1 Protein possibly involved in assembly of actin patches Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches C IDA actin cortical patch GO_0030479 3297 YJL020W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL020C/BBC1 3298 YJL022W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 3299 YJL023C PET130 Protein required for respiratory growth Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3300 YJL024C APS3 Small subunit of the clathrin-associated adaptor complex AP-3 Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress C IMP AP-3 adaptor complex GO_0030123 3301 YJL025W RRN7 Component of the core factor (CF) rDNA transcription factor complex Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p C IDA; IDA RNA polymerase I core factor complex; nucleolus GO_0070860; GO_0005730 3302 YJL026C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL026W/RNR2 3303 YJL026W RNR2 Ribonucleotide-diphosphate reductase (RNR), small subunit Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; ribonucleoside-diphosphate reductase complex; cytoplasm GO_0005634; GO_0005971; GO_0005737 3304 YJL027C YJL027C Putative protein of unknown function Putative protein of unknown function 3305 YJL028W YJL028W Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments C HDA ribosome GO_0005840 3306 YJL029C VPS53 Component of the GARP (Golgi-associated retrograde protein) complex Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p, C HDA; HDA; IDA; IPI cytoplasm; cytosol; Golgi apparatus; GARP complex GO_0005737; GO_0005829; GO_0005794; GO_0000938 3307 YJL030W MAD2 Component of the spindle-assembly checkpoint complex Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability C IPI; IDA; IDA mitotic checkpoint complex; nuclear pore; condensed nuclear chromosome kinetochore GO_0033597; GO_0005643; GO_0000778 3308 YJL031C BET4 Alpha subunit of Type II geranylgeranyltransferase Alpha subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p C IDA; IDA; HDA Rab-protein geranylgeranyltransferase complex; peroxisome; cytosol GO_0005968; GO_0005777; GO_0005829 3309 YJL032W Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase 3310 YJL033W HCA4 DEAD box RNA helicase DEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis C IMP small-subunit processome GO_0032040 3311 YJL034W KAR2 ATPase involved in protein import into the ER ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p C IDA; IDA endoplasmic reticulum; luminal surveillance complex GO_0005783; GO_0034099 3312 YJL035C TAD2 Subunit of tRNA-specific adenosine-34 deaminase Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3313 YJL036W SNX4 Sorting nexin Sorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p C IDA; IDA; IDA; IDA early endosome; pre-autophagosomal structure; extrinsic component of membrane; cytosol GO_0005769; GO_0000407; GO_0019898; GO_0005829 3314 YJL037W IRC18 Protein involved in outer spore wall assembly Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, LOH1/YJL038C, and the double mutant irc18 loh1 exhibits reduced dityrosine fluorescence relative to the single mutants; null mutant displays increased levels of spontaneous Rad52p foci C HDA fungal-type vacuole GO_0000324 3315 YJL038C LOH1 Protein involved in outer spore wall assembly Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; proposed role in maintenance of genome integrity; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to adjacent ORF, IRC18/YJL037W, and the double mutant irc18 loh1 exhibits reduced dityrosine fluorescence relative to the single mutants C HDA cytosol GO_0005829 3316 YJL039C NUP192 Essential subunit of inner ring of nuclear pore complex (NPC) Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 C IDA; IDA; HDA nuclear pore; nuclear pore inner ring; nucleus GO_0005643; GO_0044611; GO_0005634 3317 YJL041W NSP1 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p) C IDA; HDA; IDA; IDA nuclear pore nuclear basket; nuclear periphery; nuclear pore central transport channel; nuclear pore GO_0044615; GO_0034399; GO_0044613; GO_0005643 3318 YJL042W MHP1 Microtubule-associated protein involved in microtubule organization Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins C IDA microtubule GO_0005874 3319 YJL043W YJL043W Putative protein of unknown function Putative protein of unknown function; YJL043W is a non-essential gene C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 3320 YJL044C GYP6 GTPase-activating protein (GAP) for yeast Rab family member Ypt6p GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport C IDA; IDA; HDA; HDA endosome; trans-Golgi network; clathrin-coated vesicle; cytosol GO_0005768; GO_0005802; GO_0030136; GO_0005829 3321 YJL045W YJL045W Minor succinate dehydrogenase isozyme Minor succinate dehydrogenase isozyme; participates in oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner; YJL045W has a paralog, SDH1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 3322 YJL046W AIM22 Putative lipoate-protein ligase Putative lipoate-protein ligase; required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss C IDA mitochondrion GO_0005739 3323 YJL047C RTT101 Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p C HDA; HDA; IDA nucleus; cytoplasm; Cul8-RING ubiquitin ligase complex GO_0005634; GO_0005737; GO_0035361 3324 YJL047C-A YJL047C-A Putative protein of unknown function Putative protein of unknown function 3325 YJL048C UBX6 UBX (ubiquitin regulatory X) domain-containing protein UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication C IDA nucleus GO_0005634 3326 YJL049W CHM7 Yeast homolog of human CHMP7, localizes to the endoplasmic reticulum Putative protein of unknown function; YJL049W is a non-essential gene 3327 YJL050W MTR4 ATP-dependent 3'-5' RNA helicase of the DExD/H family ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing C IDA; IPI; IDA nucleus; TRAMP complex; nucleolus GO_0005634; GO_0031499; GO_0005730 3328 YJL051W IRC8 Bud tip localized protein of unknown function Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc C IDA; HDA; HDA cellular bud; cell periphery; fungal-type vacuole GO_0005933; GO_0071944; GO_0000324 3329 YJL052C-A YJL052C-A Putative protein of unknown function Putative protein of unknown function; identified based on comparison to related yeast species 3330 YJL052W TDH1 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria C IPI; HDA; IDA; HDA; IDA mitochondrion; cytosol; lipid particle; plasma membrane; fungal-type cell wall GO_0005739; GO_0005829; GO_0005811; GO_0005886; GO_0009277 3331 YJL053W PEP8 Vacuolar protein component of the retromer Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress C HDA; HDA; IMP; IMP; IPI fungal-type vacuole membrane; cytoplasm; retromer complex; retromer, cargo-selective complex; endosome GO_0000329; GO_0005737; GO_0030904; GO_0030906; GO_0005768 3332 YJL054W TIM54 Component of the mitochondrial TIM22 complex Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane C HDA; IDA mitochondrion; mitochondrial inner membrane protein insertion complex GO_0005739; GO_0042721 3333 YJL055W YJL055W Putative protein of unknown function Putative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3334 YJL056C ZAP1 Zinc-regulated transcription factor Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains C IDA nucleus GO_0005634 3335 YJL057C IKS1 Protein kinase of unknown cellular role Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p 3336 YJL058C BIT61 Subunit of TORC2 membrane-associated complex Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication C IDA; HDA; IDA; HDA plasma membrane; cell periphery; TORC2 complex; cytoplasm GO_0005886; GO_0071944; GO_0031932; GO_0005737 3337 YJL059W YHC3 Protein required for the ATP-dependent transport of arginine Protein required for the ATP-dependent transport of arginine; vacuolar membrane protein; involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) C IDA fungal-type vacuole GO_0000324 3338 YJL060W BNA3 Kynurenine aminotransferase Kynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate C IDA; IDA mitochondrion; cytoplasm GO_0005739; GO_0005737 3339 YJL061W NUP82 Linker nucleoporin component of the nuclear pore complex (NPC) Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA nucleus; nuclear pore cytoplasmic filaments; cytosol; nuclear pore; nuclear pore linkers GO_0005634; GO_0044614; GO_0005829; GO_0005643; GO_0044612 3340 YJL062W LAS21 Integral plasma membrane protein Integral plasma membrane protein; involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity C HDA; IDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane; integral component of plasma membrane GO_0005783; GO_0030176; GO_0005887 3341 YJL062W-A COA3 Mitochondrial protein required for cytochrome c oxidase assembly Mitochondrial protein required for cytochrome c oxidase assembly; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; located in the mitochondrial inner membrane C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; integral component of mitochondrial inner membrane GO_0005739; GO_0005743; GO_0031305 3342 YJL063C MRPL8 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 3343 YJL064W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 3344 YJL065C DLS1 Subunit of ISW2/yCHRAC chromatin accessibility complex Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication C HDA; IDA nucleus; CHRAC GO_0005634; GO_0008623 3345 YJL066C MPM1 Mitochondrial intermembrane space protein of unknown function Mitochondrial intermembrane space protein of unknown function C HDA; IDA; IDA mitochondrion; mitochondrial intermembrane space; extrinsic component of membrane GO_0005739; GO_0005758; GO_0019898 3346 YJL067W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3347 YJL068C YJL068C Esterase that can function as an S-formylglutathione hydrolase Esterase that can function as an S-formylglutathione hydrolase; non-essential intracellular esterase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D C IDA cytosol GO_0005829 3348 YJL069C UTP18 Small-subunit processome protein involved in pre-18S rRNA maturation Small-subunit processome protein involved in pre-18S rRNA maturation; part of a subunit of the 90S preribosomal particle capable of interacting directly with the 5' ETS of the 35S pre-rRNA; contains WD40 repeats C IDA; IDA; HDA; HDA Pwp2p-containing subcomplex of 90S preribosome; small-subunit processome; nucleolus; 90S preribosome GO_0034388; GO_0032040; GO_0005730; GO_0030686 3349 YJL070C YJL070C Putative metallo-dependent hydrolase superfamily protein Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 3350 YJL071W ARG2 Acetylglutamate synthase (glutamate N-acetyltransferase) Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p C IDA mitochondrial matrix GO_0005759 3351 YJL072C PSF2 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery C IPI; IPI; IDA; IDA; IDA; IDA GINS complex; DNA replication preinitiation complex; nucleus; replication fork protection complex; nuclear replication fork; CMG complex GO_0000811; GO_0031261; GO_0005634; GO_0031298; GO_0043596; GO_0071162 3352 YJL073W JEM1 DnaJ-like chaperone required for nuclear membrane fusion during mating DnaJ-like chaperone required for nuclear membrane fusion during mating; localizes to the ER membrane; exhibits genetic interactions with KAR2 C HDA; IDA endoplasmic reticulum; nuclear outer membrane-endoplasmic reticulum membrane network GO_0005783; GO_0042175 3353 YJL074C SMC3 Subunit of the multiprotein cohesin complex Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member C IDA; IDA nucleus; nuclear mitotic cohesin complex GO_0005634; GO_0034990 3354 YJL075C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate 3355 YJL076W NET1 Core subunit of the RENT complex Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication C IDA; IDA nucleolus; RENT complex GO_0005730; GO_0030869 3356 YJL077C ICS3 Protein with a role in copper homeostasis Protein with a role in processing of secretory proteins; possible role in vacuolar sorting, null mutants are hypersensitive to sortin2 3357 YJL077W-A YJL077W-A Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data; completely overlaps the verified gene YJL077C/ICS3 3358 YJL077W-B YJL077W-B Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3359 YJL078C PRY3 Cell wall-associated protein involved in export of acetylated sterols Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 3360 YJL079C PRY1 Sterol binding protein involved in the export of acetylated sterols Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication C HDA; IDA; HDA; HDA fungal-type vacuole; extracellular region; nuclear envelope; endoplasmic reticulum GO_0000324; GO_0005576; GO_0005635; GO_0005783 3361 YJL080C SCP160 Essential RNA-binding G protein effector of mating response pathway Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins C HDA; IDA; IDA; IDA; IDA; IDA fungal-type vacuole membrane; polysome; endoplasmic reticulum; endoplasmic reticulum membrane; cytoplasm; nuclear outer membrane-endoplasmic reticulum membrane network GO_0000329; GO_0005844; GO_0005783; GO_0005789; GO_0005737; GO_0042175 3362 YJL081C ARP4 Nuclear actin-related protein involved in chromatin remodeling Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes C IDA; IDA; IDA; IPI; IDA NuA4 histone acetyltransferase complex; nuclear chromatin; Swr1 complex; Ino80 complex; nucleus GO_0035267; GO_0000790; GO_0000812; GO_0031011; GO_0005634 3363 YJL082W IML2 Protein required for clearance of inclusion bodies Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication C HDA; HDA; HDA; HDA mitochondrion; nucleus; cytoplasm; mitochondrial outer membrane GO_0005739; GO_0005634; GO_0005737; GO_0005741 3364 YJL083W TAX4 EH domain-containing protein EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication C IDA pre-autophagosomal structure GO_0000407 3365 YJL084C ALY2 Alpha arrestin Alpha arrestin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication C HDA; IDA; HDA; IDA cytosol; early endosome; cytoplasm; late endosome GO_0005829; GO_0005769; GO_0005737; GO_0005770 3366 YJL085W EXO70 Subunit of the exocyst complex Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress C IDA; HDA; IDA; IDA; HDA; IDA; HDA; HDA cellular bud tip; cytoplasm; plasma membrane; exocyst; cellular bud neck; incipient cellular bud site; mating projection tip; prospore membrane GO_0005934; GO_0005737; GO_0005886; GO_0000145; GO_0005935; GO_0000131; GO_0043332; GO_0005628 3367 YJL086C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 3368 YJL087C TRL1 tRNA ligase tRNA ligase; required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes C IDA; IDA polysome; nuclear inner membrane GO_0005844; GO_0005637 3369 YJL088W ARG3 Ornithine carbamoyltransferase Ornithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline C IDA cytosol GO_0005829 3370 YJL089W SIP4 C6 zinc cluster transcriptional activator C6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus C IDA nucleus GO_0005634 3371 YJL090C DPB11 DNA replication initiation protein DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress C IDA; IDA; HDA; IMP epsilon DNA polymerase complex; replication fork; nucleus; DNA replication preinitiation complex GO_0008622; GO_0005657; GO_0005634; GO_0031261 3372 YJL091C GWT1 Protein involved in the inositol acylation of GlcN-PI Protein involved in the inositol acylation of GlcN-PI; the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) forms glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors C IDA; HDA; HDA integral component of membrane; endoplasmic reticulum; nuclear outer membrane-endoplasmic reticulum membrane network GO_0016021; GO_0005783; GO_0042175 3373 YJL092W SRS2 DNA helicase and DNA-dependent ATPase DNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1 C IDA nucleus GO_0005634 3374 YJL093C TOK1 Outward-rectifier potassium channel of the plasma membrane Outward-rectifier potassium channel of the plasma membrane; has two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin C IMP; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 3375 YJL094C KHA1 Putative K+/H+ antiporter Putative K+/H+ antiporter; has a probable role in intracellular cation homeostasis; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies C HDA; IDA; HDA endoplasmic reticulum; Golgi apparatus; mitochondrion GO_0005783; GO_0005794; GO_0005739 3376 YJL095W BCK1 MAPKKK acting in the protein kinase C signaling pathway MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase C HDA; HDA; HDA mating projection tip; cytoplasm; cellular bud neck GO_0043332; GO_0005737; GO_0005935 3377 YJL096W MRPL49 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 3378 YJL097W PHS1 Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking C HDA; IDA; HDA; HDA; HDA fungal-type vacuole; integral component of endoplasmic reticulum membrane; endoplasmic reticulum; fungal-type vacuole membrane; nuclear outer membrane-endoplasmic reticulum membrane network GO_0000324; GO_0030176; GO_0005783; GO_0000329; GO_0042175 3379 YJL098W SAP185 Protein that forms a complex with the Sit4p protein phosphatase Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication C HDA; IPI mitochondrion; cytoplasm GO_0005739; GO_0005737 3380 YJL099W CHS6 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA clathrin-coated vesicle; exomer complex; trans-Golgi network transport vesicle; cytosol GO_0030136; GO_0034044; GO_0030140; GO_0005829 3381 YJL100W LSB6 Type II phosphatidylinositol 4-kinase Type II phosphatidylinositol 4-kinase; binds Las17p, a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization C IDA; IDA; HDA fungal-type vacuole membrane; plasma membrane; cytoplasm GO_0000329; GO_0005886; GO_0005737 3382 YJL101C GSH1 Gamma glutamylcysteine synthetase Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress C IDA intracellular GO_0005622 3383 YJL102W MEF2 Mitochondrial elongation factor involved in translational elongation Mitochondrial elongation factor involved in translational elongation C HDA; HDA mitochondrion; cytosol GO_0005739; GO_0005829 3384 YJL103C GSM1 Putative zinc cluster protein of unknown function Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis 3385 YJL104W PAM16 Subunit of the import motor (PAM complex) Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain C HDA; IDA; IDA mitochondrion; presequence translocase-associated import motor; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0001405; GO_0005744 3386 YJL105W SET4 Protein of unknown function, contains a SET domain Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication 3387 YJL106W IME2 Serine/threonine protein kinase involved in activation of meiosis Serine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p C IDA nucleus GO_0005634 3388 YJL107C YJL107C Putative protein of unknown function Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi 3389 YJL108C PRM10 Pheromone-regulated protein Pheromone-regulated protein; proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation C HDA endoplasmic reticulum GO_0005783 3390 YJL109C UTP10 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance C HDA; IDA; IDA; IDA; IDA; IDA; IDA mitochondrion; 90S preribosome; nucleolus; rDNA heterochromatin; small-subunit processome; t-UTP complex; preribosome, small subunit precursor GO_0005739; GO_0030686; GO_0005730; GO_0033553; GO_0032040; GO_0034455; GO_0030688 3391 YJL110C GZF3 GATA zinc finger protein GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication C IDA nucleus GO_0005634 3392 YJL111W CCT7 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance C HDA; IPI cytoplasm; chaperonin-containing T-complex GO_0005737; GO_0005832 3393 YJL112W MDV1 Peripheral protein of cytosolic face of mitochondrial outer membrane Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication C HDA; HDA mitochondrial outer membrane; mitochondrion GO_0005741; GO_0005739 3394 YJL113W YJL113W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA; HDA nucleus; mitochondrion GO_0005634; GO_0005739 3395 YJL114W YJL114W Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 3396 YJL115W ASF1 Nucleosome assembly factor Nucleosome assembly factor; involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition; relocalizes to the cytosol in response to hypoxia C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 3397 YJL116C NCA3 Protein involved in mitochondrion organization Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 3398 YJL117W PHO86 Endoplasmic reticulum (ER) resident protein Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress C HDA endoplasmic reticulum GO_0005783 3399 YJL118W YJL118W Protein of unknown function Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein C HDA ribosome GO_0005840 3400 YJL119C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3401 YJL120W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO 3402 YJL121C RPE1 D-ribulose-5-phosphate 3-epimerase D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress 3403 YJL122W ALB1 Shuttling pre-60S factor Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p C HDA; HDA; IDA nucleus; ribosome; cytoplasm GO_0005634; GO_0005840; GO_0005737 3404 YJL123C MTC1 Protein of unknown function that may interact with ribosomes Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 C HDA; HDA; HDA; HDA cytoplasm; COPI-coated vesicle; ribosome; Golgi apparatus GO_0005737; GO_0030137; GO_0005840; GO_0005794 3405 YJL124C LSM1 Lsm (Like Sm) protein Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; IDA; IPI; IDA P-body; cytoplasm; Lsm1-7-Pat1 complex; mRNA cap binding complex; nucleus GO_0000932; GO_0005737; GO_1990726; GO_0005845; GO_0005634 3406 YJL125C GCD14 Subunit of tRNA (1-methyladenosine) methyltransferase Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression C IDA; IDA tRNA (m1A) methyltransferase complex; nucleus GO_0031515; GO_0005634 3407 YJL126W NIT2 Nit protein Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member 3408 YJL127C SPT10 Histone H3 acetylase with a role in transcriptional regulation Putative histone acetylase with a role in transcriptional silencing; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box C HDA nucleus GO_0005634 3409 YJL127C-B MCO6 Putative protein of unknown function Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii C HDA mitochondrion GO_0005739 3410 YJL127W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3411 YJL128C PBS2 MAP kinase kinase of the HOG signaling pathway MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition C IDA; IDA; IDA; IDA cytoplasmic stress granule; cellular bud tip; cytoplasm; cellular bud neck GO_0010494; GO_0005934; GO_0005737; GO_0005935 3412 YJL129C TRK1 Component of the Trk1p-Trk2p potassium transport system Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication C HDA; IDA; IDA cell periphery; plasma membrane; membrane raft GO_0071944; GO_0005886; GO_0045121 3413 YJL130C URA2 Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP C HDA; IDA; IDA mitochondrion; cytoplasm; integral component of membrane GO_0005739; GO_0005737; GO_0016021 3414 YJL131C AIM23 Mitochondrial translation initiation factor 3 (IF3, mIF3) Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 3415 YJL132W YJL132W Putative protein of unknown function Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene C IDA; HDA membrane; fungal-type vacuole GO_0016020; GO_0000324 3416 YJL133C-A DPI8 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3417 YJL133W MRS3 Iron transporter, mediates Fe2+ transport across inner mito membrane Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 3418 YJL134W LCB3 Long-chain base-1-phosphate phosphatase Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 3419 YJL135W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 3420 YJL136C RPS21B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 3421 YJL136W-A YJL136W-A Putative protein of unknown function Putative protein of unknown function; identified by SAGE 3422 YJL137C GLG2 Glycogenin glucosyltransferase Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3423 YJL138C TIF2 Translation initiation factor eIF4A Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication C IGI; HDA; HDA; IDA eukaryotic translation initiation factor 4F complex; plasma membrane; cytoplasm; cytoplasmic stress granule GO_0016281; GO_0005886; GO_0005737; GO_0010494 3424 YJL139C YUR1 Mannosyltransferase involved in protein N-glycosylation Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication C IDA; HDA Golgi apparatus; endoplasmic reticulum GO_0005794; GO_0005783 3425 YJL140W RPB4 RNA polymerase II subunit B32 RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation C IDA; IDA; IDA; IDA DNA-directed RNA polymerase II, core complex; P-body; cytoplasm; nucleus GO_0005665; GO_0000932; GO_0005737; GO_0005634 3426 YJL141C YAK1 Serine-threonine protein kinase Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3427 YJL142C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci 3428 YJL143W TIM17 Essential component of the TIM23 complex Essential component of the TIM23 complex; with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core Translocase of the Inner Mitochondrial membrane (TIM23 complex) C HDA; IDA mitochondrion; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0005744 3429 YJL144W YJL144W Cytoplasmic hydrophilin essential in desiccation-rehydration process Cytoplasmic hydrophilin essential in desiccation-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 3430 YJL145W SFH5 Non-classical phosphatidylinositol transfer protein (PITP) Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress C IDA; HDA; IDA; IDA; HDA; HDA; HDA cytosol; fungal-type vacuole membrane; plasma membrane; cortical endoplasmic reticulum; cell periphery; nucleus; cytoplasm GO_0005829; GO_0000329; GO_0005886; GO_0032541; GO_0071944; GO_0005634; GO_0005737 3431 YJL146W IDS2 Protein involved in modulation of Ime2p activity during meiosis Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3432 YJL147C MRX5 Protein that associates with mitochondrial ribosome Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene C HDA mitochondrion GO_0005739 3433 YJL148W RPA34 RNA polymerase I subunit A34.5 RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p C IDA; IDA; IDA DNA-directed RNA polymerase I complex; nucleolus; nucleus GO_0005736; GO_0005730; GO_0005634 3434 YJL149W DAS1 Putative SCF ubiquitin ligase F-box protein Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C C IDA SCF ubiquitin ligase complex GO_0019005 3435 YJL150W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3436 YJL151C SNA3 Protein involved in efficient MVB sorting of proteins to the vacuole Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles C IDA; IDA fungal-type vacuole lumen; fungal-type vacuole GO_0000328; GO_0000324 3437 YJL152W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3438 YJL153C INO1 Inositol-3-phosphate synthase Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element C HDA cytoplasm GO_0005737 3439 YJL154C VPS35 Endosomal subunit of membrane-associated retromer complex Endosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p C IPI; IPI; IMP; IDA endosome; retromer, cargo-selective complex; retromer complex; fungal-type vacuole membrane GO_0005768; GO_0030906; GO_0030904; GO_0000329 3440 YJL155C FBP26 Fructose-2,6-bisphosphatase, required for glucose metabolism Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 3441 YJL156C SSY5 Serine protease of SPS plasma membrane amino acid sensor system Serine protease of SPS plasma membrane amino acid sensor system; contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p; other members are Ssy1p and Ptr3p C IDA extrinsic component of plasma membrane GO_0019897 3442 YJL156W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3443 YJL157C FAR1 CDK inhibitor and nuclear anchor CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites C HDA; IDA; IDA cytoplasm; nucleus; mating projection tip GO_0005737; GO_0005634; GO_0043332 3444 YJL158C CIS3 Mannose-containing glycoprotein constituent of the cell wall Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family C HDA; HDA; HDA; IDA; IDA; HDA; IDA endoplasmic reticulum; plasma membrane; fungal-type vacuole; fungal-type cell wall; extracellular region; cell periphery; cellular bud tip GO_0005783; GO_0005886; GO_0000324; GO_0009277; GO_0005576; GO_0071944; GO_0005934 3445 YJL159W HSP150 O-mannosylated heat shock protein O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication C IDA; HDA; IDA; IDA fungal-type cell wall; cytosol; cellular bud scar; extracellular region GO_0009277; GO_0005829; GO_0005621; GO_0005576 3446 YJL160C PIR5 Putative protein of unknown function Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 3447 YJL161W FMP33 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3448 YJL162C JJJ2 Protein of unknown function Protein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3449 YJL163C YJL163C Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole membrane GO_0000329 3450 YJL164C TPK1 cAMP-dependent protein kinase catalytic subunit cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cAMP-dependent protein kinase complex; cytoplasm; nuclear chromatin; nucleus GO_0005952; GO_0005737; GO_0000790; GO_0005634 3451 YJL165C HAL5 Putative protein kinase Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication 3452 YJL166W QCR8 Subunit 8 of ubiquinol cytochrome-c reductase (Complex III) Subunit 8 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p C HDA; IDA mitochondrion; mitochondrial respiratory chain complex III GO_0005739; GO_0005750 3453 YJL167W ERG20 Farnesyl pyrophosphate synthetase Farnesyl pyrophosphate synthetase; has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis C IDA; IDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 3454 YJL168C SET2 Histone methyltransferase with a role in transcriptional elongation Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 3455 YJL169W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 3456 YJL170C ASG7 Protein that regulates signaling from G protein beta subunit Ste4p Protein that regulates signaling from G protein beta subunit Ste4p; contributes to relocalization of Ste4p within the cell; specific to a-cells and induced by alpha-factor C IDA plasma membrane GO_0005886 3457 YJL171C TOH1 GPI-anchored cell wall protein of unknown function GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress C IDA; HDA; HDA; HDA fungal-type cell wall; mitochondrion; plasma membrane; endoplasmic reticulum GO_0009277; GO_0005739; GO_0005886; GO_0005783 3458 YJL172W CPS1 Vacuolar carboxypeptidase S Vacuolar carboxypeptidase S; expression is induced under low-nitrogen conditions C HDA; IDA fungal-type vacuole; fungal-type vacuole lumen GO_0000324; GO_0000328 3459 YJL173C RFA3 Subunit of heterotrimeric Replication Protein A (RPA) Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress C IDA; IDA; IMP DNA replication factor A complex; condensed nuclear chromosome; chromosome, telomeric region GO_0005662; GO_0000794; GO_0000781 3460 YJL174W KRE9 Glycoprotein involved in cell wall beta-glucan assembly Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects C HDA; IDA fungal-type vacuole; extracellular region GO_0000324; GO_0005576 3461 YJL175W Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor 3462 YJL176C SWI3 Subunit of the SWI/SNF chromatin remodeling complex Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions C IDA; IDA; IDA SWI/SNF complex; cytosol; nucleus GO_0016514; GO_0005829; GO_0005634 3463 YJL177W RPL17B Ribosomal 60S subunit protein L17B Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication C IDA; IDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 3464 YJL178C ATG27 Type I membrane protein involved in autophagy and the Cvt pathway Type I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site C IDA; IDA; IDA; IDA; HDA; IDA pre-autophagosomal structure; trans-Golgi network; vacuolar membrane; membrane; fungal-type vacuole; mitochondrion GO_0000407; GO_0005802; GO_0005774; GO_0016020; GO_0000324; GO_0005739 3465 YJL179W PFD1 Subunit of heterohexameric prefoldin Subunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation C IMP prefoldin complex GO_0016272 3466 YJL180C ATP12 Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase Assembly factor for the F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for the assembly of alpha and beta subunits into the F1 sector of the mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency C HDA mitochondrion GO_0005739 3467 YJL181W RBH1 Putative protein of unknown function Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication 3468 YJL182C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W 3469 YJL183W MNN11 Subunit of a Golgi mannosyltransferase complex Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p C IPI; HDA alpha-1,6-mannosyltransferase complex; endoplasmic reticulum GO_0000136; GO_0005783 3470 YJL184W GON7 Component of the EKC/KEOPS protein complex Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; implicated in osmotic stress response; other complex members are Kae1p, Cgi121p, Pcc1p, and Bud32p C IDA; IDA EKC/KEOPS complex; nuclear chromatin GO_0000408; GO_0000790 3471 YJL185C ATG36 Pex3p interacting protein, required for pexophagy Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene C IDA peroxisome GO_0005777 3472 YJL186W MNN5 Alpha-1,2-mannosyltransferase Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment C IDA Golgi apparatus GO_0005794 3473 YJL187C SWE1 Protein kinase that regulates the G2/M transition Protein kinase that regulates the G2/M transition; regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; phosphorylates conserved tyrosine residue in N-terminus of Hsp90 in cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; homolog of S. pombe Wee1p; potential Cdc28p substrate C IDA; IDA nucleus; cellular bud neck GO_0005634; GO_0005935 3474 YJL188C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay 3475 YJL189W RPL39 Ribosomal 60S subunit protein L39 Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog C IDA cytosolic large ribosomal subunit GO_0022625 3476 YJL190C RPS22A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 3477 YJL191W RPS14B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication C IDA small-subunit processome GO_0032040 3478 YJL192C SOP4 ER-membrane protein ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER C IDA; IDA endoplasmic reticulum; integral component of membrane GO_0005783; GO_0016021 3479 YJL193W YJL193W Putative protein of unknown function Putative protein of unknown function; predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect 3480 YJL194W CDC6 Essential ATP-binding protein required for DNA replication Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress C IDA; HDA; HDA; IDA DNA replication preinitiation complex; cytoplasm; nucleus; nuclear pre-replicative complex GO_0031261; GO_0005737; GO_0005634; GO_0005656 3481 YJL195C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 3482 YJL196C ELO1 Elongase I, medium-chain acyl elongase Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 3483 YJL197C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL197C/UBP12 3484 YJL197W UBP12 Ubiquitin-specific protease Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3485 YJL198W PHO90 Low-affinity phosphate transporter Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 3486 YJL199C MBB1 Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies 3487 YJL200C ACO2 Putative mitochondrial aconitase isozyme Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol C HDA mitochondrion GO_0005739 3488 YJL201W ECM25 Non-essential protein of unknown function Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p C HDA cytoplasm GO_0005737 3489 YJL202C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex 3490 YJL203W PRP21 Subunit of the SF3a splicing factor complex Subunit of the SF3a splicing factor complex; required for spliceosome assembly C IDA U2-type prespliceosome GO_0071004 3491 YJL204C RCY1 F-box protein involved in recycling endocytosed proteins F-box protein involved in recycling endocytosed proteins; involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth; direct interaction with C-terminal cytoplasmic region of Drs2p plays an important role for Drs2p function in endocytic recycling pathway C IDA; IDA; IDA; HDA site of polarized growth; Golgi apparatus; endosome; cellular bud GO_0030427; GO_0005794; GO_0005768; GO_0005933 3492 YJL205C NCE101 Protein of unknown function Protein of unknown function; involved in secretion of proteins that lack classical secretory signal sequences C HDA cytosol GO_0005829 3493 YJL206C YJL206C Putative protein of unknown function Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment 3494 YJL207C LAA1 AP-1 accessory protein AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene C IPI; HDA; HDA AP-1 adaptor complex; ribosome; clathrin-coated vesicle GO_0030121; GO_0005840; GO_0030136 3495 YJL208C NUC1 Major mitochondrial nuclease Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; nucleus GO_0005739; GO_0005743; GO_0005634 3496 YJL209W CBP1 Mitochondrial protein, regulator of COB mRNA stability and translation Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress C HDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0031314 3497 YJL210W PEX2 RING-finger peroxin and E3 ubiquitin ligase RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import C IDA; IDA peroxisomal importomer complex; peroxisomal membrane GO_1990429; GO_0005778 3498 YJL211C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 3499 YJL212C OPT1 Proton-coupled oligopeptide transporter of the plasma membrane Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family C IDA integral component of plasma membrane GO_0005887 3500 YJL213W YJL213W Protein of unknown function that may interact with ribosomes Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p C HDA ribosome GO_0005840 3501 YJL214W HXT8 Protein of unknown function with similarity to hexose transporters Protein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose C HDA cell periphery GO_0071944 3502 YJL215C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3503 YJL216C IMA5 Alpha-glucosidase Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; most distant member of the IMA isomaltase family, but with similar catalytic properties as Ima1p and Ima2p; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose; can cleave alpha-1,3 linkage of nigerose and turanose and alpha-1,5 linkage of leucrose and is very sensitive to temperature in vitro 3504 YJL217W REE1 Cytoplasmic protein involved in the regulation of enolase (ENO1) Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle C HDA cytoplasm GO_0005737 3505 YJL218W YJL218W Putative acetyltransferase Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene 3506 YJL219W HXT9 Putative hexose transporter that is nearly identical to Hxt11p Putative hexose transporter that is nearly identical to Hxt11p; has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p C HDA; HDA cell periphery; fungal-type vacuole GO_0071944; GO_0000324 3507 YJL220W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL221C/FSP2 3508 YJL221C IMA4 Alpha-glucosidase Alpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima4p overexpression allows the ima1 null mutant to grow on isomaltose; identical to IMA3 3509 YJL222W VTH2 Putative membrane glycoprotein Putative membrane glycoprotein; has strong similarity to Vth1p and Pep1p/Vps10p; may be involved in vacuolar protein sorting C HDA fungal-type vacuole GO_0000324 3510 YJL222W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3511 YJL222W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3512 YJL223C PAU1 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis, negatively regulated by oxygen; repressed by heme; identical to Pau14p C HDA fungal-type vacuole GO_0000324 3513 YJL225C YJL225C Putative Y' element ATP-dependent helicase Putative Y' element ATP-dependent helicase 3514 YJL225W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YJL225C 3515 YJR001W AVT1 Vacuolar transporter Vacuolar transporter; imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters C IMP; IDA fungal-type vacuole membrane; fungal-type vacuole GO_0000329; GO_0000324 3516 YJR002W MPP10 Component of the SSU processome and 90S preribosome Component of the SSU processome and 90S preribosome; required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p C IDA; HDA; IDA; HDA; HDA small-subunit processome; nucleus; Mpp10 complex; 90S preribosome; nucleolus GO_0032040; GO_0005634; GO_0034457; GO_0030686; GO_0005730 3517 YJR003C MRX12 Protein that associates with mitochondrial ribosome Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis C HDA mitochondrion GO_0005739 3518 YJR004C SAG1 Alpha-agglutinin of alpha-cells Alpha-agglutinin of alpha-cells; binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor C HDA; IDA; HDA fungal-type vacuole; fungal-type cell wall; cell periphery GO_0000324; GO_0009277; GO_0071944 3519 YJR005C-A LSO1 Protein with a potential role in response to iron deprivation Putative protein of unknown function; transcription increases during treatment with 2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and copper, and may be regulated by Aft1p; originally identified as a syntenic homolog of an Ashbya gossypii gene; LSO1 has a paralog, LSO2, that arose from the whole genome duplication C IDA nucleus GO_0005634 3520 YJR005W APL1 Beta-adaptin Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex C IDA; IDA; HDA endocytic vesicle; AP-2 adaptor complex; cellular bud neck GO_0030139; GO_0030122; GO_0005935 3521 YJR006W POL31 Subunit of DNA polymerase delta (polymerase III) Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 3522 YJR007W SUI2 Alpha subunit of the translation initiation factor eIF2 Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA; IDA; IMP cytoplasmic stress granule; multi-eIF complex; cytoplasm; eukaryotic 48S preinitiation complex; eukaryotic translation initiation factor 2 complex GO_0010494; GO_0043614; GO_0005737; GO_0033290; GO_0005850 3523 YJR008W MHO1 Protein of unknown function Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3524 YJR009C TDH2 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; IDA; IDA; HDA cytoplasm; cytosol; fungal-type cell wall; nucleus; lipid particle; mitochondrion; plasma membrane GO_0005737; GO_0005829; GO_0009277; GO_0005634; GO_0005811; GO_0005739; GO_0005886 3525 YJR010C-A SPC1 Subunit of the signal peptidase complex (SPC) Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress C HDA; IDA endoplasmic reticulum; signal peptidase complex GO_0005783; GO_0005787 3526 YJR010W MET3 ATP sulfurylase ATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 3527 YJR011C YJR011C Putative protein of unknown function Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS 3528 YJR012C YJR012C Essential protein of unknown function Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W 3529 YJR013W GPI14 Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I; involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M C IPI; HDA glycosylphosphatidylinositol-mannosyltransferase I complex; endoplasmic reticulum GO_1990529; GO_0005783 3530 YJR014W TMA22 Protein of unknown function Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress C HDA; IDA cytoplasm; ribosome GO_0005737; GO_0005840 3531 YJR015W YJR015W Putative protein of unknown function Putative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 3532 YJR016C ILV3 Dihydroxyacid dehydratase Dihydroxyacid dehydratase; catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids C HDA mitochondrion GO_0005739 3533 YJR017C ESS1 Peptidylprolyl-cis/trans-isomerase (PPIase) Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 3534 YJR018W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3535 YJR019C TES1 Peroxisomal acyl-CoA thioesterase Peroxisomal acyl-CoA thioesterase; likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids C HDA; IDA mitochondrion; peroxisome GO_0005739; GO_0005777 3536 YJR020W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3537 YJR021C REC107 Protein involved in early stages of meiotic recombination Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation C IDA condensed nuclear chromosome GO_0000794 3538 YJR022W LSM8 Lsm (Like Sm) protein Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA C IDA; IDA; IDA; IDA nucleus; U4/U6 x U5 tri-snRNP complex; U6 snRNP; nucleolus GO_0005634; GO_0046540; GO_0005688; GO_0005730 3539 YJR023C Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing 3540 YJR024C MDE1 5'-methylthioribulose-1-phosphate dehydratase 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant C HDA cytoplasm GO_0005737 3541 YJR025C BNA1 3-hydroxyanthranilic acid dioxygenase 3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p C IDA cytoplasm GO_0005737 3542 YJR026W YJR026W Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 3543 YJR027W YJR027W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 3544 YJR028W YJR028W Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 3545 YJR029W YJR029W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 3546 YJR030C RBH2 Putative protein of unknown function Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; non-essential gene; YJR030C has a paralog, YJL181W, that arose from the whole genome duplication 3547 YJR031C GEA1 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication C HDA; IDA; IPI; IDA cellular bud; Golgi cis cisterna; Golgi-associated vesicle; extrinsic component of membrane GO_0005933; GO_0000137; GO_0005798; GO_0019898 3548 YJR032W CPR7 Peptidyl-prolyl cis-trans isomerase (cyclophilin) Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants C IPI cytosol GO_0005829 3549 YJR033C RAV1 Subunit of RAVE complex (Rav1p, Rav2p, Skp1p) Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate C IPI; IDA; IDA RAVE complex; extrinsic component of membrane; cytoplasm GO_0043291; GO_0019898; GO_0005737 3550 YJR034W PET191 Protein required for assembly of cytochrome c oxidase Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery C HDA; IDA; IDA mitochondrion; integral component of mitochondrial inner membrane; mitochondrial intermembrane space GO_0005739; GO_0031305; GO_0005758 3551 YJR035W RAD26 Protein involved in transcription-coupled nucleotide excision repair Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3552 YJR036C HUL4 Protein with similarity to hect domain E3 ubiquitin-protein ligases Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex C HDA; IDA prospore membrane; TRAMP complex GO_0005628; GO_0031499 3553 YJR037W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces 3554 YJR038C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3555 YJR039W MLO127 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3556 YJR040W GEF1 Voltage-gated chloride channel Voltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism C IDA; IDA; IDA; IDA; IDA; IDA plasma membrane; Golgi medial cisterna; fungal-type vacuole; endosome; endoplasmic reticulum; Golgi apparatus GO_0005886; GO_0005797; GO_0000324; GO_0005768; GO_0005783; GO_0005794 3557 YJR041C URB2 Protein required for normal metabolism of the rRNA primary transcript Protein required for normal metabolism of the rRNA primary transcript; nucleolar protein; proposed to be involved in ribosome biogenesis C IDA nucleolus GO_0005730 3558 YJR042W NUP85 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 C IDA; IDA nuclear pore; nuclear pore outer ring GO_0005643; GO_0031080 3559 YJR043C POL32 Third subunit of DNA polymerase delta Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p C HDA nucleus GO_0005634 3560 YJR044C VPS55 Late endosomal protein involved in late endosome to vacuole transport Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP) C IDA; IDA Vps55/Vps68 complex; late endosome GO_0034424; GO_0005770 3561 YJR045C SSC1 Hsp70 family ATPase Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication C IDA; IDA; HDA; IDA presequence translocase-associated import motor; mitochondrial nucleoid; mitochondrion; mitochondrial inner membrane GO_0001405; GO_0042645; GO_0005739; GO_0005743 3562 YJR046W TAH11 DNA replication licensing factor DNA replication licensing factor; required for pre-replication complex assembly C IDA; IDA; IDA nuclear pre-replicative complex; cytoplasm; nucleus GO_0005656; GO_0005737; GO_0005634 3563 YJR047C ANB1 Translation elongation factor eIF-5A Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication 3564 YJR048W CYC1 Cytochrome c, isoform 1 Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; mutations in human homolog cause insulin-responsive hyperglycemia; CYC1 has a paralog, CYC7, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial intermembrane space GO_0005739; GO_0005758 3565 YJR049C UTR1 ATP-NADH kinase ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3566 YJR050W ISY1 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles C IDA; IDA; IDA; IDA; IDA U2-type catalytic step 1 spliceosome; post-spliceosomal complex; Prp19 complex; U2-type catalytic step 2 spliceosome; post-mRNA release spliceosomal complex GO_0071006; GO_0071020; GO_0000974; GO_0071007; GO_0071014 3567 YJR051W OSM1 Fumarate reductase, catalyzes the reduction of fumarate to succinate Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication C HDA; IDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 3568 YJR052W RAD7 Nucleotide excision repair (NER) protein Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex C IDA; IDA Cul3-RING ubiquitin ligase complex; nucleotide-excision repair factor 4 complex GO_0031463; GO_0000113 3569 YJR053W BFA1 Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p Component of the GTPase-activating Bfa1p-Bub2p complex; involved in multiple cell cycle checkpoint pathways that control exit from mitosis; specifically required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p C IPI; IDA Bfa1-Bub2 complex; spindle pole body GO_1990334; GO_0005816 3570 YJR054W KCH1 Potassium transporter that mediates K+ influx Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; localized to sites of polarized growth; member of a fungal-specific gene family; potential Cdc28p substrate; KCH1 has a paralog, PRM6, that arose from the whole genome duplication C IDA; IDA; HDA mating projection; integral component of plasma membrane; fungal-type vacuole GO_0005937; GO_0005887; GO_0000324 3571 YJR055W HIT1 Protein involved in C/D snoRNP assembly Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3 C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3572 YJR056C YJR056C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3573 YJR057W CDC8 Nucleoside monophosphate and nucleoside diphosphate kinase Thymidylate and uridylate kinase; functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3574 YJR058C APS2 Small subunit of the clathrin-associated adaptor complex AP-2 Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex C HDA; HDA; IDA; HDA; IDA cellular bud neck; prospore membrane; AP-2 adaptor complex; nucleus; clathrin-coated vesicle GO_0005935; GO_0005628; GO_0030122; GO_0005634; GO_0030136 3575 YJR059W PTK2 Serine/threonine protein kinase Putative serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication C IDA; IDA; IDA plasma membrane; nucleus; cytoplasm GO_0005886; GO_0005634; GO_0005737 3576 YJR060W CBF1 Basic helix-loop-helix (bHLH) protein Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress C HDA; IPI; IDA; IDA; HDA mitochondrion; kinetochore; Cbf1-Met4-Met28 complex; chromosome, centromeric region; nucleus GO_0005739; GO_0000776; GO_0089713; GO_0000775; GO_0005634 3577 YJR061W MNN14 Putative protein of unknown function Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication 3578 YJR062C NTA1 Amidase Amidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation C HDA mitochondrion GO_0005739 3579 YJR063W RPA12 RNA polymerase I subunit A12.2 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex C IDA DNA-directed RNA polymerase I complex GO_0005736 3580 YJR064W CCT5 Subunit of the cytosolic chaperonin Cct ring complex Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo C IDA chaperonin-containing T-complex GO_0005832 3581 YJR065C ARP3 Essential component of the Arp2/3 complex Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity C IDA; IDA; HDA Arp2/3 protein complex; actin cortical patch; plasma membrane GO_0005885; GO_0030479; GO_0005886 3582 YJR066W TOR1 PIK-related protein kinase and rapamycin target PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication C IDA; IDA; IPI; IDA; IDA; IDA; IDA; IDA fungal-type vacuole membrane; plasma membrane; TORC1 complex; extrinsic component of cytoplasmic side of plasma membrane; nucleus; endosome membrane; cytoplasm; Golgi membrane GO_0000329; GO_0005886; GO_0031931; GO_0031234; GO_0005634; GO_0010008; GO_0005737; GO_0000139 3583 YJR067C YAE1 Protein that forms a complex with Lto1p and Rli1p Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3584 YJR068W RFC2 Subunit of heteropentameric Replication factor C (RF-C) Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon C IPI; IPI; IDA; IDA; IDA Elg1 RFC-like complex; Ctf18 RFC-like complex; Rad17 RFC-like complex; DNA replication factor C complex; nucleus GO_0031391; GO_0031390; GO_0031389; GO_0005663; GO_0005634 3585 YJR069C HAM1 Nucleoside triphosphate pyrophosphohydrolase Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimidines from deoxyribonucleoside triphosphate pools; functions together with YJL055W to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA without affecting uptake or incorporation of uracil into RNA; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3586 YJR070C LIA1 Deoxyhypusine hydroxylase Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3587 YJR071W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3588 YJR072C NPA3 Member of the conserved GPN-loop GTPase family Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress C IPI; HDA; HDA DNA-directed RNA polymerase II, core complex; cytoplasm; cytosol GO_0005665; GO_0005737; GO_0005829 3589 YJR073C OPI3 Methylene-fatty-acyl-phospholipid synthase Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase C HDA; HDA; HDA mitochondrion; cell periphery; endoplasmic reticulum GO_0005739; GO_0071944; GO_0005783 3590 YJR074W MOG1 Conserved nuclear protein that interacts with GTP-Gsp1p Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p C IDA nucleus GO_0005634 3591 YJR075W HOC1 Alpha-1,6-mannosyltransferase Alpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele C IDA alpha-1,6-mannosyltransferase complex GO_0000136 3592 YJR076C CDC11 Component of the septin ring that is required for cytokinesis Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells C IDA; IDA; IDA; IPI; IDA; IDA; HDA; IDA; HDA; IDA; IDA cellular bud neck septin ring; spindle microtubule; meiotic spindle; septin complex; septin filament array; mating projection base; mating projection tip; ascospore wall; cellular bud neck; cytoplasmic microtubule; prospore membrane GO_0000144; GO_0005876; GO_0072687; GO_0031105; GO_0032160; GO_0001400; GO_0043332; GO_0005619; GO_0005935; GO_0005881; GO_0005628 3593 YJR077C MIR1 Mitochondrial phosphate carrier Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 3594 YJR078W BNA2 Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase; required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp C HDA cytoplasm GO_0005737 3595 YJR079W YJR079W Putative protein of unknown function Putative protein of unknown function; mutation results in impaired mitochondrial respiration 3596 YJR080C AIM24 Protein with a role in determining mitochondrial architecture Protein with a role in determining mitochondrial architecture; inner membrane protein that interacts physically and genetically with the MICOS complex and is required for its integrity C IDA; HDA integral component of mitochondrial inner membrane; mitochondrion GO_0031305; GO_0005739 3597 YJR082C EAF6 Subunit of the NuA4 acetyltransferase complex Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 C IDA; IDA; HDA; IDA; IDA NuA3 histone acetyltransferase complex; NuA3a histone acetyltransferase complex; nucleus; NuA4 histone acetyltransferase complex; NuA3b histone acetyltransferase complex GO_0033100; GO_1990467; GO_0005634; GO_0035267; GO_1990468 3598 YJR083C ACF4 Protein of unknown function Protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate 3599 YJR084W YJR084W Protein that forms a complex with Thp3p Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome C IDA transcriptionally active chromatin GO_0035327 3600 YJR085C TMH11 Protein of unknown function Protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 3601 YJR086W STE18 G protein gamma subunit G protein gamma subunit; forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling C IDA; IDA; IDA; HDA plasma membrane; heterotrimeric G-protein complex; cytoplasm; cell periphery GO_0005886; GO_0005834; GO_0005737; GO_0071944 3602 YJR087W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 3603 YJR088C EMC2 Member of conserved ER transmembrane complex Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 C HDA; HDA; IDA nucleus; endoplasmic reticulum; ER membrane protein complex GO_0005634; GO_0005783; GO_0072546 3604 YJR089W BIR1 Subunit of chromosomal passenger complex (CPC) Subunit of chromosomal passenger complex (CPC); CPC is comprised of Ipl1p-Sli15p-Bir1p-Nbl1p and regulates chromosome segregation; required for chromosome bi-orientation and for spindle assembly checkpoint activation upon reduced sister kinetochore tension; relative distribution to shortened microtubules increases upon DNA replication stress; sumoylated in an Mms21p-dependent manner; human survivin homolog C IGI; IDA; IDA; HDA; IDA; IDA; IDA; HDA condensed nuclear chromosome kinetochore; chromosome passenger complex; nucleus; mitochondrion; spindle midzone; cytoplasm; spindle; microtubule GO_0000778; GO_0032133; GO_0005634; GO_0005739; GO_0051233; GO_0005737; GO_0005819; GO_0005874 3605 YJR090C GRR1 F-box protein component of an SCF ubiquitin-ligase complex F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification C IDA; IDA; IDA; IMP cellular bud neck contractile ring; cytoplasm; nucleus; SCF ubiquitin ligase complex GO_0000142; GO_0005737; GO_0005634; GO_0019005 3606 YJR091C JSN1 Member of the Puf family of RNA-binding proteins Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication C IDA; IDA; IDA fungal-type cell wall; cytoplasm; cytoplasmic side of mitochondrial outer membrane GO_0009277; GO_0005737; GO_0032473 3607 YJR092W BUD4 Anillin-like protein involved in bud-site selection Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p C IDA; HDA; IDA cellular bud neck contractile ring; cellular bud neck; cellular bud scar GO_0000142; GO_0005935; GO_0005621 3608 YJR093C FIP1 Subunit of cleavage polyadenylation factor (CPF) Subunit of cleavage polyadenylation factor (CPF); interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p C IDA mRNA cleavage and polyadenylation specificity factor complex GO_0005847 3609 YJR094C IME1 Master regulator of meiosis that is active only during meiotic events Master regulator of meiosis that is active only during meiotic events; activates transcription of early meiotic genes through interaction with Ume6p; degraded by the 26S proteasome following phosphorylation by Ime2p; transcription is negatively regulated in cis by the IRT1 long noncoding antisense RNA C IDA nucleus GO_0005634 3610 YJR094W-A RPL43B Ribosomal 60S subunit protein L43B Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA cytosolic large ribosomal subunit GO_0022625 3611 YJR095W SFC1 Mitochondrial succinate-fumarate transporter Mitochondrial succinate-fumarate transporter; transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization C HDA mitochondrion GO_0005739 3612 YJR096W YJR096W Xylose and arabinose reductase Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3613 YJR097W JJJ3 Protein of unknown function Protein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3614 YJR098C YJR098C Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 3615 YJR099W YUH1 Ubiquitin C-terminal hydrolase Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p 3616 YJR100C AIM25 Mitochondria protein of unknown function Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 3617 YJR101W RSM26 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 3618 YJR102C VPS25 Component of the ESCRT-II complex Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome C HDA; IDA cytosol; ESCRT II complex GO_0005829; GO_0000814 3619 YJR103W URA8 Minor CTP synthase isozyme (see also URA7) Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication C HDA; IDA fungal-type vacuole; cytoophidium GO_0000324; GO_0097268 3620 YJR104C SOD1 Cytosolic copper-zinc superoxide dismutase Cytosolic copper-zinc superoxide dismutase; detoxifies superoxide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p and enters the nucleus under oxidative stress to promote transcription of stress response genes; human ortholog implicated in ALS; abundance increases under DNA replication stress and during exposure to boric acid; localization of a fraction to the mitochondrial intermembrane space is modulated by the MICOS complex C IDA; HDA; IDA; IDA mitochondrial intermembrane space; mitochondrion; nucleus; cytosol GO_0005758; GO_0005739; GO_0005634; GO_0005829 3621 YJR105W ADO1 Adenosine kinase Adenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3622 YJR106W ECM27 Protein involved in calcium homeostasis and exit from quiescence Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p C HDA endoplasmic reticulum GO_0005783 3623 YJR107W LIH1 Putative lipase Putative lipase 3624 YJR108W ABM1 Protein of unknown function Protein of unknown function; required for normal microtubule organization 3625 YJR109C CPA2 Large subunit of carbamoyl phosphate synthetase Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor C IDA carbamoyl-phosphate synthase complex GO_0005951 3626 YJR110W YMR1 Phosphatidylinositol 3-phosphate (PI3P) phosphatase Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family C HDA cytoplasm GO_0005737 3627 YJR111C PXP2 Peroxisomal matrix protein with naturally active promoter Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria C IDA; HDA peroxisome; mitochondrion GO_0005777; GO_0005739 3628 YJR112W NNF1 Essential component of the MIND kinetochore complex Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) C IDA; IDA nuclear MIS12/MIND complex; kinetochore GO_0000818; GO_0000776 3629 YJR112W-A YJR112W-A Putative protein of unknown function Putative protein of unknown function; identified based on homology to Ashbya gossypii C HDA endoplasmic reticulum GO_0005783 3630 YJR113C RSM7 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 3631 YJR114W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSM7/YJR113C 3632 YJR115W YJR115W Putative protein of unknown function Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication 3633 YJR116W TDA4 Putative protein of unknown function Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele C HDA endoplasmic reticulum GO_0005783 3634 YJR117W STE24 Highly conserved zinc metalloprotease Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans C HDA; HDA; IMP; IDA endoplasmic reticulum; mitochondrial outer membrane; nuclear inner membrane; integral component of endoplasmic reticulum membrane GO_0005783; GO_0005741; GO_0005637; GO_0030176 3635 YJR118C ILM1 Protein of unknown function Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth C HDA endoplasmic reticulum GO_0005783 3636 YJR119C JHD2 JmjC domain family histone demethylase JmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3637 YJR120W YJR120W Protein of unknown function Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p 3638 YJR121W ATP2 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated C IDA; HDA mitochondrial proton-transporting ATP synthase, catalytic core; mitochondrion GO_0005754; GO_0005739 3639 YJR122W IBA57 Protein involved in incorporating iron-sulfur clusters into proteins Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 3640 YJR123W RPS5 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 C HDA; IDA 90S preribosome; cytosolic small ribosomal subunit GO_0030686; GO_0022627 3641 YJR124C YJR124C Putative protein of unknown function Putative protein of unknown function; expression induced under calcium shortage C HDA fungal-type vacuole membrane GO_0000329 3642 YJR125C ENT3 Protein containing an N-terminal epsin-like domain Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p C IDA; IDA clathrin vesicle coat; endosome GO_0030125; GO_0005768 3643 YJR126C VPS70 Protein of unknown function involved in vacuolar protein sorting Protein of unknown function involved in vacuolar protein sorting C HDA endoplasmic reticulum GO_0005783 3644 YJR127C RSF2 Zinc-finger protein Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3645 YJR128W YJR128W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 3646 YJR129C EFM3 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A C HDA cytoplasm GO_0005737 3647 YJR130C STR2 Cystathionine gamma-synthase, converts cysteine into cystathionine Cystathionine gamma-synthase, converts cysteine into cystathionine; STR2 has a paralog, YML082W, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3648 YJR131W MNS1 Alpha-1,2-mannosidase Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation C IDA endoplasmic reticulum GO_0005783 3649 YJR132W NMD5 Karyopherin Karyopherin; a carrier protein involved in nuclear import of proteins; importin beta homolog C HDA; HDA; IDA cytoplasm; nuclear periphery; nucleus GO_0005737; GO_0034399; GO_0005634 3650 YJR133W XPT1 Xanthine-guanine phosphoribosyl transferase Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine C HDA cytoplasm GO_0005737 3651 YJR134C SGM1 Protein of unknown function Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus C HDA; HDA Golgi apparatus; COPI-coated vesicle GO_0005794; GO_0030137 3652 YJR135C MCM22 Outer kinetochore protein and component of the Ctf3 subcomplex Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 C IDA condensed nuclear chromosome kinetochore GO_0000778 3653 YJR135W-A TIM8 Mitochondrial intermembrane space protein Mitochondrial intermembrane space protein; forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome C IDA; HDA mitochondrial intermembrane space protein transporter complex; mitochondrion GO_0042719; GO_0005739 3654 YJR136C TTI2 Subunit of the ASTRA complex, involved in chromatin remodeling Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti2p; may interact with Rsm23p; GFP-fusion protein localizes to the cytoplasm C HDA; HDA ASTRA complex; cytoplasm GO_0070209; GO_0005737 3655 YJR137C MET5 Sulfite reductase beta subunit Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine C HDA; HDA; IDA; IDA; HDA fungal-type vacuole; cell periphery; sulfite reductase complex (NADPH); cytoplasmic stress granule; cytoplasm GO_0000324; GO_0071944; GO_0009337; GO_0010494; GO_0005737 3656 YJR138W IML1 GTPase-activating protein (GAP) subunit of the Iml1p/SEACIT complex GTPase-activating protein (GAP), subunit of SEA and Iml1p complexes; SEA (Seh1-associated) complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; localized to either pre-autophagosomal structures (PAS) or non-PAS structures during NNS-induced autophagy; C IDA; HDA; IDA; IDA extrinsic component of fungal-type vacuolar membrane; fungal-type vacuole membrane; GATOR1 complex; Seh1-associated complex GO_0097042; GO_0000329; GO_1990130; GO_0035859 3657 YJR139C HOM6 Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase) Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase); dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3658 YJR140C HIR3 Subunit of the HIR complex Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein C IPI; IDA HIR complex; nucleus GO_0000417; GO_0005634 3659 YJR140W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene HIR3; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3660 YJR141W IPA1 Protein implicated in pre-mRNA processing and proteasomal degradation Essential protein of unknown function 3661 YJR142W YJR142W 8-oxo-dGTP diphosphatase of the Nudix hydrolase family 8-oxo-dGTP diphosphatase of the Nudix hydrolase family; converts diphosphates of damaged forms of thiamin to monophosphates; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition 3662 YJR143C PMT4 Protein O-mannosyltransferase Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals C IPI; HDA dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex; endoplasmic reticulum GO_0097586; GO_0005783 3663 YJR144W MGM101 Protein with a role in mitochondrial DNA recombinational repair Protein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 3664 YJR145C RPS4A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication C HDA; HDA; IDA 90S preribosome; cytoplasm; cytosolic small ribosomal subunit GO_0030686; GO_0005737; GO_0022627 3665 YJR146W YJR146W Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 3666 YJR147W HMS2 Protein with similarity to heat shock transcription factors Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3667 YJR148W BAT2 Cytosolic branched-chain amino acid (BCAA) aminotransferase Cytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication C IDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3668 YJR149W YJR149W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3669 YJR150C DAN1 Cell wall mannoprotein Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 3670 YJR151C DAN4 Cell wall mannoprotein Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth C IDA; HDA fungal-type cell wall; cell periphery GO_0009277; GO_0071944 3671 YJR151W-A YJR151W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis 3672 YJR152W DAL5 Allantoate permease Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression C HDA; HDA; IMP cell periphery; fungal-type vacuole; plasma membrane GO_0071944; GO_0000324; GO_0005886 3673 YJR153W PGU1 Endo-polygalacturonase Endo-polygalacturonase; pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins C IDA extracellular region GO_0005576 3674 YJR154W YJR154W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3675 YJR155W AAD10 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 3676 YJR156C THI11 Protein involved in synthesis of the thiamine precursor HMP Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP 3677 YJR157W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3678 YJR158W HXT16 Putative transmembrane polyol transporter Protein of unknown function with similarity to hexose transporters; expression is repressed by high levels of glucose C HDA cell periphery GO_0071944 3679 YJR159W SOR1 Sorbitol dehydrogenase Sorbitol dehydrogenase; expression is induced in the presence of sorbitol or xylose 3680 YJR160C MPH3 Alpha-glucoside permease Alpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 3681 YJR161C COS5 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA; IDA fungal-type vacuole; endosome GO_0000324; GO_0005768 3682 YJR162C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3683 YKL001C MET14 Adenylylsulfate kinase Adenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism C HDA cytoplasm GO_0005737 3684 YKL002W DID4 Class E Vps protein of the ESCRT-III complex Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis C IDA; IDA ESCRT III complex; cytoplasm GO_0000815; GO_0005737 3685 YKL003C MRP17 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 3686 YKL004W AUR1 Phosphatidylinositol:ceramide phosphoinositol transferase Phosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase C IPI; HDA; IDA inositol phosphoceramide synthase complex; endoplasmic reticulum; Golgi apparatus GO_0070916; GO_0005783; GO_0005794 3687 YKL005C BYE1 Negative regulator of transcription elongation Negative regulator of transcription elongation; contains a TFIIS-like domain that associates with chromatin and a PHD domain that interacts with H3K4me3; multicopy suppressor of temperature-sensitive ess1 mutations, binds RNA polymerase II large subunit 3688 YKL006C-A SFT1 Intra-Golgi v-SNARE Intra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment C IDA; IDA SNARE complex; Golgi trans cisterna GO_0031201; GO_0000138 3689 YKL006W RPL14A Ribosomal 60S subunit protein L14A Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 3690 YKL007W CAP1 Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p) Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress C IMP; IPI; IDA; HDA; IDA; IDA; HDA; HDA F-actin capping protein complex; actin filament; cellular bud tip; plasma membrane; actin cortical patch; incipient cellular bud site; mating projection tip; cellular bud neck GO_0008290; GO_0005884; GO_0005934; GO_0005886; GO_0030479; GO_0000131; GO_0043332; GO_0005935 3691 YKL008C LAC1 Ceramide synthase component Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication C HDA; IDA nuclear periphery; acyl-CoA ceramide synthase complex GO_0034399; GO_0061576 3692 YKL009W MRT4 Protein involved in mRNA turnover and ribosome assembly Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus C IDA; HDA; HDA; IDA preribosome, large subunit precursor; nucleolus; nucleus; nucleoplasm GO_0030687; GO_0005730; GO_0005634; GO_0005654 3693 YKL010C UFD4 Ubiquitin-protein ligase (E3) Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; HDA nucleus; mitochondrion; cytoplasm GO_0005634; GO_0005739; GO_0005737 3694 YKL011C CCE1 Mitochondrial cruciform cutting endonuclease Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication C IDA mitochondrial inner membrane GO_0005743 3695 YKL012W PRP40 U1 snRNP protein involved in splicing U1 snRNP protein involved in splicing; interacts with the branchpoint-binding protein during the formation of the second commitment complex C IMP; IDA; IDA nucleus; U1 snRNP; U2-type prespliceosome GO_0005634; GO_0005685; GO_0071004 3696 YKL013C ARC19 Subunit of the ARP2/3 complex Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches C IDA Arp2/3 protein complex GO_0005885 3697 YKL014C URB1 Protein required for the normal accumulation of 25S and 5.8S rRNAs Protein required for the normal accumulation of 25S and 5.8S rRNAs; nucleolar protein; associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit C IDA nucleolus GO_0005730 3698 YKL015W PUT3 Transcriptional activator Transcriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain C HDA nucleus GO_0005634 3699 YKL016C ATP7 Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis C IMP; HDA mitochondrial proton-transporting ATP synthase, stator stalk; mitochondrion GO_0000274; GO_0005739 3700 YKL017C HCS1 Hexameric DNA polymerase alpha-associated DNA helicase A Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities C IDA; IDA alpha DNA polymerase:primase complex; DNA helicase A complex GO_0005658; GO_0033203 3701 YKL018C-A MCO12 Putative protein of unknown function Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3702 YKL018W SWD2 Subunit of the COMPASS (Set1C) histone H3K4 methyltransferase complex Subunit of the COMPASS (Set1C) histone H3K4 methyltransferase complex; required for Set1C stability and optimal activity; COMPASS methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination C IDA; IPI mRNA cleavage and polyadenylation specificity factor complex; Set1C/COMPASS complex GO_0005847; GO_0048188 3703 YKL019W RAM2 Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (RAM2-RAM1 heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (RAM2-CDC43 heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor C IDA; IDA protein farnesyltransferase complex; CAAX-protein geranylgeranyltransferase complex GO_0005965; GO_0005953 3704 YKL020C SPT23 ER membrane protein involved in regulation of OLE1 transcription ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication C HDA; IDA; IDA endoplasmic reticulum; nucleus; integral component of endoplasmic reticulum membrane GO_0005783; GO_0005634; GO_0030176 3705 YKL021C MAK11 Protein involved in an early step of 60S ribosomal subunit biogenesis Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats C IDA membrane GO_0016020 3706 YKL022C CDC16 Subunit of the anaphase-promoting complex/cyclosome (APC/C) Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA nucleus; anaphase-promoting complex; cytosol GO_0005634; GO_0005680; GO_0005829 3707 YKL023C-A MIN9 Putative protein of unknown function Putative protein of unknown function 3708 YKL023W YKL023W Putative protein of unknown function Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3709 YKL024C URA6 Uridylate kinase Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3710 YKL025C PAN3 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes C HDA; IDA cytoplasm; PAN complex GO_0005737; GO_0031251 3711 YKL026C GPX1 Phospholipid hydroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; GPX1 has a paralog, HYR1, that arose from the whole genome duplication C IDA; HDA; IDA peroxisomal matrix; cytosol; extrinsic component of mitochondrial outer membrane GO_0005782; GO_0005829; GO_0031315 3712 YKL027W TCD2 tRNA threonylcarbamoyladenosine dehydratase tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 3713 YKL028W TFA1 TFIIE large subunit TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening C HDA; IDA; IDA mitochondrion; transcription factor TFIIE complex; transcriptional preinitiation complex GO_0005739; GO_0005673; GO_0097550 3714 YKL029C MAE1 Mitochondrial malic enzyme Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids C HDA mitochondrion GO_0005739 3715 YKL030W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 3716 YKL031W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species 3717 YKL032C IXR1 Transcriptional repressor that regulates hypoxic genes during normoxia Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication C IDA nuclear chromatin GO_0000790 3718 YKL033W TTI1 Subunit of the ASTRA complex, involved in chromatin remodeling Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies C HDA; HDA; HDA cytoplasm; ASTRA complex; mitochondrion GO_0005737; GO_0070209; GO_0005739 3719 YKL033W-A YKL033W-A Putative protein of unknown function Putative protein of unknown function; similar to uncharacterized proteins from other fungi 3720 YKL034W TUL1 Subunit of the DSC ubiquitin ligase complex Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 C IDA; IDA; HDA Dsc E3 ubiquitin ligase complex; Golgi apparatus; fungal-type vacuole GO_0044695; GO_0005794; GO_0000324 3721 YKL035W UGP1 UDP-glucose pyrophosphorylase (UGPase) UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication C HDA; IDA; HDA cytoplasm; cytoplasmic stress granule; plasma membrane GO_0005737; GO_0010494; GO_0005886 3722 YKL036C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W 3723 YKL037W AIM26 Protein of unknown function Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT C IDA mitochondrion GO_0005739 3724 YKL038W RGT1 Glucose-responsive transcription factor Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication C IDA nucleus GO_0005634 3725 YKL039W PTM1 Protein of unknown function Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication 3726 YKL040C NFU1 Protein involved in Fe-S cluster transfer to mitochondrial clients Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 3727 YKL041W VPS24 One of four subunits of the ESCRT-III complex One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway C HDA; IDA; IDA cytosol; ESCRT III complex; cytoplasm GO_0005829; GO_0000815; GO_0005737 3728 YKL042W SPC42 Central plaque component of spindle pole body (SPB) Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane C IDA; IDA central plaque of spindle pole body; intermediate layer of spindle pole body GO_0005823; GO_0005821 3729 YKL043W PHD1 Transcriptional activator that enhances pseudohyphal growth Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication C IDA nucleus GO_0005634 3730 YKL044W MMO1 Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data C HDA mitochondrion GO_0005739 3731 YKL045W PRI2 Subunit of DNA primase Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair C IDA; IDA; IDA alpha DNA polymerase:primase complex; nuclear envelope; nucleus GO_0005658; GO_0005635; GO_0005634 3732 YKL046C DCW1 Putative mannosidase Putative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p C IDA; HDA; HDA anchored component of plasma membrane; cellular bud; endoplasmic reticulum GO_0046658; GO_0005933; GO_0005783 3733 YKL047W ANR2 Protein of unknown function Putative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated C IDA; HDA; HDA lipid particle; cytoplasm; endoplasmic reticulum GO_0005811; GO_0005737; GO_0005783 3734 YKL048C ELM1 Serine/threonine protein kinase Serine/threonine protein kinase that regulates cellular morphogenesis; septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring C IDA; HDA cellular bud neck septin ring; cellular bud neck GO_0000144; GO_0005935 3735 YKL049C CSE4 Centromeric histone H3-like protein Centromere protein that resembles histone H3; associated with promoters, accessible chromatin and RNA polymerase II-bound regions; phosphorylated Cse4p associates with centromeres; required for proper kinetochore function; levels regulated by E3 ubiquitin ligase Psh1p; phosphorylation of Cse4p may destabilize defective kinetochores to promote bi-orientation; ubiquitination of N terminus regulates Cse4p proteolysis for faithful chromosome segregation; human CENP-A homolog C IDA; IGI; IDA; IDA 2-micrometer circle DNA; kinetochore; condensed nuclear chromosome, centromeric region; CENP-A containing nucleosome GO_0005729; GO_0000776; GO_0000780; GO_0043505 3736 YKL050C YKL050C Protein of unknown function Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p; YKL050C has a paralog, EIS1, that arose from the whole genome duplication 3737 YKL051W SFK1 Plasma membrane protein that may act to generate normal levels of PI4P Plasma membrane protein that may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane C HDA; IMP cell periphery; plasma membrane GO_0071944; GO_0005886 3738 YKL052C ASK1 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress C IDA DASH complex GO_0042729 3739 YKL053C-A MDM35 Mitochondrial intermembrane space protein Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA; HDA nucleus; mitochondrial intermembrane space; cytoplasm; mitochondrion GO_0005634; GO_0005758; GO_0005737; GO_0005739 3740 YKL053W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 3741 YKL054C DEF1 RNAPII degradation factor RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis C IMP nucleus GO_0005634 3742 YKL055C OAR1 Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p C HDA mitochondrion GO_0005739 3743 YKL056C TMA19 Protein that associates with ribosomes Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress C HDA; IDA; IDA; IDA; IDA cytoplasm; cytoplasmic stress granule; ribosome; cytosol; mitochondrion GO_0005737; GO_0010494; GO_0005840; GO_0005829; GO_0005739 3744 YKL057C NUP120 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 C IDA; IDA nuclear pore outer ring; nuclear pore GO_0031080; GO_0005643 3745 YKL058W TOA2 TFIIA small subunit TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa1p, for ribosomal protein gene transcription in vivo; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress C IDA transcription factor TFIIA complex GO_0005672 3746 YKL059C MPE1 Essential conserved subunit of CPF cleavage and polyadenylation factor Essential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif; relocalizes to the cytosol in response to hypoxia C IDA; IPI; IDA nucleus; mRNA cleavage and polyadenylation specificity factor complex; cytosol GO_0005634; GO_0005847; GO_0005829 3747 YKL060C FBA1 Fructose 1,6-bisphosphate aldolase Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo C IDA; HDA; IDA mitochondrion; cytoplasm; cytosol GO_0005739; GO_0005737; GO_0005829 3748 YKL061W BLI1 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome C IDA; HDA BLOC-1 complex; endosome GO_0031083; GO_0005768 3749 YKL062W MSN4 Stress-responsive transcriptional activator Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 3750 YKL063C YKL063C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi C HDA Golgi apparatus GO_0005794 3751 YKL064W MNR2 Vacuolar membrane protein required for magnesium homeostasis Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations C HDA; IDA fungal-type vacuole membrane; vacuole-mitochondrion membrane contact site GO_0000329; GO_1990816 3752 YKL065C YET1 Endoplasmic reticulum transmembrane protein Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication C IDA; HDA endoplasmic reticulum; ribosome GO_0005783; GO_0005840 3753 YKL065W-A DPC7 Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 3754 YKL066W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 3755 YKL067W YNK1 Nucleoside diphosphate kinase Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA cytosol; mitochondrion; mitochondrial intermembrane space GO_0005829; GO_0005739; GO_0005758 3756 YKL068W NUP100 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication C IDA; IDA nuclear pore; nuclear pore central transport channel GO_0005643; GO_0044613 3757 YKL068W-A YKL068W-A Putative protein of unknown function Putative protein of unknown function; identified by homology to Ashbya gossypii 3758 YKL069W YKL069W Methionine-R-sulfoxide reductase Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; HDA cytoplasm; cytosol; nucleus GO_0005737; GO_0005829; GO_0005634 3759 YKL070W YKL070W Putative protein of unknown function Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3760 YKL071W YKL071W Putative protein of unknown function Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3761 YKL072W STB6 Protein that binds Sin3p in a two-hybrid assay Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication 3762 YKL073W LHS1 Molecular chaperone of the endoplasmic reticulum lumen Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway C IDA; HDA endoplasmic reticulum lumen; endoplasmic reticulum GO_0005788; GO_0005783 3763 YKL074C MUD2 Protein involved in early pre-mRNA splicing Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 C IDA commitment complex GO_0000243 3764 YKL075C YKL075C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin C HDA cytoplasm GO_0005737 3765 YKL076C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C 3766 YKL077W PSG1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole C HDA; HDA fungal-type vacuole; endoplasmic reticulum GO_0000324; GO_0005783 3767 YKL078W DHR2 Predominantly nucleolar DEAH-box ATP-dependent RNA helicase Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis C IDA nucleolus GO_0005730 3768 YKL079W SMY1 Kinesin-like myosin passenger-protein Kinesin-like myosin passenger-protein; interacts with Myo2p; controls actin cable structure and dynamics; proposed to be involved in exocytosis C IDA; IDA; IDA; IDA cellular bud tip; incipient cellular bud site; mating projection tip; cellular bud neck GO_0005934; GO_0000131; GO_0043332; GO_0005935 3769 YKL080W VMA5 Subunit C of the V1 peripheral membrane domain of V-ATPase Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits C HDA fungal-type vacuole membrane GO_0000329 3770 YKL081W TEF4 Gamma subunit of translational elongation factor eEF1B Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex C HDA; IDA mitochondrion; cytoplasmic stress granule GO_0005739; GO_0010494 3771 YKL082C RRP14 Essential protein, constituent of 66S pre-ribosomal particles Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family C IDA; IDA nucleolus; cytosolic large ribosomal subunit GO_0005730; GO_0022625 3772 YKL083W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 3773 YKL084W HOT13 Zinc-binding mitochondrial intermembrane space (IMS) protein Zinc-binding mitochondrial intermembrane space (IMS) protein; involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc C IDA mitochondrial intermembrane space GO_0005758 3774 YKL085W MDH1 Mitochondrial malate dehydrogenase Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated C IPI; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 3775 YKL086W SRX1 Sulfiredoxin Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3776 YKL087C CYT2 Cytochrome c1 heme lyase Cytochrome c1 heme lyase; involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 C HDA; IDA mitochondrion; mitochondrial intermembrane space GO_0005739; GO_0005758 3777 YKL088W CAB3 Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC C HDA; IDA; IDA cytoplasm; phosphopantothenoylcysteine decarboxylase complex; CoA-synthesizing protein complex GO_0005737; GO_0071513; GO_1990143 3778 YKL089W MIF2 Protein required for structural integrity of elongating spindles Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 C IDA; IDA condensed nuclear chromosome, centromeric region; nuclear MIS12/MIND complex GO_0000780; GO_0000818 3779 YKL090W CUE2 Protein of unknown function Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination 3780 YKL091C YKL091C Putative phosphatidylinositol/phosphatidylcholine transfer protein Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication C IDA nucleus GO_0005634 3781 YKL092C BUD2 GTPase activating factor for Rsr1p/Bud1p GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types C HDA; HDA; IDA; HDA cellular bud neck; prospore membrane; incipient cellular bud site; cytoplasm GO_0005935; GO_0005628; GO_0000131; GO_0005737 3782 YKL093W MBR1 Protein involved in mitochondrial functions and stress response Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; MBR1 has a paralog, ISF1, that arose from the whole genome duplication 3783 YKL094W YJU3 Monoglyceride lipase (MGL) Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family C IDA; HDA; HDA; IDA; HDA; HDA; HDA; HDA lipid particle; mitochondrial outer membrane; mitochondrion; membrane; plasma membrane; cytoplasm; cell periphery; endoplasmic reticulum GO_0005811; GO_0005741; GO_0005739; GO_0016020; GO_0005886; GO_0005737; GO_0071944; GO_0005783 3784 YKL095W YJU2 Essential protein required for pre-mRNA splicing Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing C IDA; HDA U2-type catalytic step 1 spliceosome; nucleus GO_0071006; GO_0005634 3785 YKL096C-B YKL096C-B Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3786 YKL096W CWP1 Cell wall mannoprotein that localizes to birth scars of daughter cells Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance C IDA; IDA; HDA; IDA cellular birth scar; prospore membrane; fungal-type vacuole; fungal-type cell wall GO_0071597; GO_0005628; GO_0000324; GO_0009277 3787 YKL096W-A CWP2 Covalently linked cell wall mannoprotein Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored C HDA; IDA cytosol; fungal-type cell wall GO_0005829; GO_0009277 3788 YKL097C YKL097C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species 3789 YKL098W MTC2 Protein of unknown function Protein of unknown function; mtc2 is synthetically sick with cdc13-1 3790 YKL099C UTP11 Subunit of U3-containing Small Subunit (SSU) processome complex Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit C IDA; IDA nucleolus; small-subunit processome GO_0005730; GO_0032040 3791 YKL100C YPF1 Intramembrane aspartyl protease of the perinuclear ER membrane Putative protein of unknown function; has similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene C IDA perinuclear endoplasmic reticulum membrane GO_1990578 3792 YKL100W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 3793 YKL101W HSL1 Nim1p-related protein kinase Nim1p-related protein kinase; regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p C IDA; IDA cellular bud neck septin ring; cellular bud neck GO_0000144; GO_0005935 3794 YKL102C YKL102C Putative protein of unknown function Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site 3795 YKL103C APE1 Vacuolar aminopeptidase yscI Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress C IDA; HDA fungal-type vacuole; cytoplasm GO_0000324; GO_0005737 3796 YKL104C GFA1 Glutamine-fructose-6-phosphate amidotransferase Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication 3797 YKL105C SEG2 Eisosome component Eisosome component; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication C HDA cell periphery GO_0071944 3798 YKL106C-A YKL106C-A Putative protein of unknown function Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi 3799 YKL106W AAT1 Mitochondrial aspartate aminotransferase Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis C HDA mitochondrion GO_0005739 3800 YKL107W YKL107W Putative short-chain dehydrogenase/reductase Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein 3801 YKL108W SLD2 Single-stranded DNA origin-binding and annealing protein Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress C IGI; HDA; HDA DNA replication preinitiation complex; nucleus; cytoplasm GO_0031261; GO_0005634; GO_0005737 3802 YKL109W HAP4 Transcription factor Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift C IDA CCAAT-binding factor complex GO_0016602 3803 YKL110C KTI12 Protein that plays a role in modification of tRNA wobble nucleosides Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3804 YKL111C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 3805 YKL112W ABF1 DNA binding protein with possible chromatin-reorganizing activity DNA binding protein with possible chromatin-reorganizing activity; involved in transcriptional activation, gene silencing, and DNA replication and repair C IMP; IDA nucleus; nucleotide-excision repair factor 4 complex GO_0005634; GO_0000113 3806 YKL113C RAD27 5' to 3' exonuclease, 5' flap endonuclease 5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA mitochondrion; nucleus; cytosol GO_0005739; GO_0005634; GO_0005829 3807 YKL114C APN1 Major apurinic/apyrimidinic endonuclease Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity C IDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 3808 YKL115C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRR1 3809 YKL116C PRR1 Serine/threonine protein kinase Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor C HDA cytoplasm GO_0005737 3810 YKL117W SBA1 Co-chaperone that binds and regulates Hsp90 family chaperones Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 3811 YKL118W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPH2 3812 YKL119C VPH2 Integral membrane protein required for V-ATPase function Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner C HDA; IPI; IDA endoplasmic reticulum; Vma12-Vma22 assembly complex; endoplasmic reticulum membrane GO_0005783; GO_1990871; GO_0005789 3813 YKL120W OAC1 Mitochondrial inner membrane transporter Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 3814 YKL121W DGR2 Protein of unknown function Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication 3815 YKL122C SRP21 Subunit of the signal recognition particle (SRP) Subunit of the signal recognition particle (SRP); SRP functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm C HDA; IDA nucleus; signal recognition particle, endoplasmic reticulum targeting GO_0005634; GO_0005786 3816 YKL123W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SSH4 3817 YKL124W SSH4 Specificity factor required for Rsp5p-dependent ubiquitination Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases C HDA; IDA endoplasmic reticulum; fungal-type vacuole GO_0005783; GO_0000324 3818 YKL125W RRN3 Protein required for transcription of rDNA by RNA polymerase I Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo C IDA nucleolus GO_0005730 3819 YKL126W YPK1 S/T protein kinase Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication C IDA; IDA; IDA cellular bud neck; plasma membrane; cytosol GO_0005935; GO_0005886; GO_0005829 3820 YKL127W PGM1 Phosphoglucomutase, minor isoform Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 3821 YKL128C PMU1 Putative phosphomutase Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3822 YKL129C MYO3 One of two type I myosins One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication C HDA cell periphery GO_0071944 3823 YKL130C SHE2 RNA-binding protein that binds specific mRNAs and interacts with She3p RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER C IDA; IDA; HDA; IDA endoplasmic reticulum membrane; cellular bud tip; cytosol; cytoplasm GO_0005789; GO_0005934; GO_0005829; GO_0005737 3824 YKL131W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3825 YKL132C RMA1 Putative dihydrofolate synthetase Putative dihydrofolate synthetase; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; RMA1 has a paralog, FOL3, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 3826 YKL133C RCI50 Putative protein of unknown function Putative protein of unknown function; not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype); YKL133C has a paralog, MGR3, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 3827 YKL134C OCT1 Mitochondrial intermediate peptidase Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 3828 YKL135C APL2 Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress C IPI; HDA AP-1 adaptor complex; cytosol GO_0030121; GO_0005829 3829 YKL136W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C 3830 YKL137W CMC1 Copper-binding protein of the mitochondrial intermembrane space Copper-binding protein of the mitochondrial intermembrane space; evolutionarily conserved; may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif C IDA; HDA; IDA extrinsic component of mitochondrial inner membrane; mitochondrion; mitochondrial intermembrane space GO_0031314; GO_0005739; GO_0005758 3831 YKL138C MRPL31 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 3832 YKL138C-A HSK3 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 3833 YKL139W CTK1 Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I) Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 C IDA; IDA; IDA; IDA; IDA nucleolus; nucleus; CTDK-1 complex; nucleoplasm; polysome GO_0005730; GO_0005634; GO_0070692; GO_0005654; GO_0005844 3834 YKL140W TGL1 Steryl ester hydrolase Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes C IDA; IDA integral component of membrane; lipid particle GO_0016021; GO_0005811 3835 YKL141W SDH3 Subunit of succinate dehydrogenase and of TIM22 translocase Subunit of succinate dehydrogenase and of TIM22 translocase; functions as cytochrome b subunit of succinate dehydrogenase, which couples oxidation of succinate to transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex; SDH3 has a paralog, SHH3, that arose from the whole genome duplication C IDA; HDA; IDA mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone); mitochondrion; mitochondrial inner membrane protein insertion complex GO_0005749; GO_0005739; GO_0042721 3836 YKL142W MRP8 Protein of unknown function Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis C HDA cytoplasm GO_0005737 3837 YKL143W LTV1 Subunit of the EGO/GSE complex Component of the GSE complex; GSE is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature C HDA; IDA; IDA; IDA; IDA cytoplasm; EGO complex; late endosome membrane; nucleus; preribosome, small subunit precursor GO_0005737; GO_0034448; GO_0031902; GO_0005634; GO_0030688 3838 YKL144C RPC25 RNA polymerase III subunit C25 RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p C IDA DNA-directed RNA polymerase III complex GO_0005666 3839 YKL145W RPT1 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance C IDA proteasome regulatory particle, base subcomplex GO_0008540 3840 YKL145W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified essential gene RPT1; identified by expression profiling and mass spectrometry 3841 YKL146W AVT3 Vacuolar transporter Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters C HDA; IDA fungal-type vacuole membrane; fungal-type vacuole GO_0000329; GO_0000324 3842 YKL147C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 3843 YKL148C SDH1 Flavoprotein subunit of succinate dehydrogenase Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; SDH1 has a paralog, YJL045W, that arose from the whole genome duplication C IDA; IDA; IDA mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone); mitochondrion; mitochondrial membrane GO_0005749; GO_0005739; GO_0031966 3844 YKL149C DBR1 RNA lariat debranching enzyme RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies 3845 YKL150W MCR1 Mitochondrial NADH-cytochrome b5 reductase Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis C IDA; HDA; IDA; IDA integral component of mitochondrial outer membrane; mitochondrion; mitochondrial outer membrane; mitochondrial intermembrane space GO_0031307; GO_0005739; GO_0005741; GO_0005758 3846 YKL151C NNR2 Widely-conserved NADHX dehydratase NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 3847 YKL152C GPM1 Tetrameric phosphoglycerate mutase Tetrameric phosphoglycerate mutase; mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis C HDA; IDA; IDA mitochondrion; cytosol; mitochondrial intermembrane space GO_0005739; GO_0005829; GO_0005758 3848 YKL153W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant 3849 YKL154W SRP102 Signal recognition particle (SRP) receptor beta subunit Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane C IPI; IDA signal recognition particle receptor complex; integral component of endoplasmic reticulum membrane GO_0005785; GO_0030176 3850 YKL155C RSM22 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 3851 YKL156C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene RPS27A; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3852 YKL156W RPS27A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; HDA cytosolic small ribosomal subunit; cytoplasm GO_0022627; GO_0005737 3853 YKL157W APE2 Aminopeptidase yscII Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication C IDA; IDA; HDA; HDA extracellular region; cell wall-bounded periplasmic space; mitochondrion; nucleus GO_0005576; GO_0030287; GO_0005739; GO_0005634 3854 YKL159C RCN1 Protein involved in calcineurin regulation during calcium signaling Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region 3855 YKL160W ELF1 Transcription elongation factor with a conserved zinc finger domain Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression C HDA; IDA nucleus; transcription elongation factor complex GO_0005634; GO_0008023 3856 YKL161C KDX1 Protein kinase Protein kinase; implicated in Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p; KDX1 has a paralog, SLT2, that arose from the whole genome duplication 3857 YKL162C YKL162C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion C HDA mitochondrion GO_0005739 3858 YKL162C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3859 YKL163W PIR3 O-glycosylated covalently-bound cell wall protein O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication C IDA fungal-type cell wall GO_0009277 3860 YKL164C PIR1 O-glycosylated protein required for cell wall stability O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication C IDA; IDA; IDA fungal-type cell wall; cellular bud scar; nuclear periphery GO_0009277; GO_0005621; GO_0034399 3861 YKL165C MCD4 Protein involved in GPI anchor synthesis Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol C IDA; HDA; IDA fungal-type cell wall; endoplasmic reticulum; fungal-type vacuole GO_0009277; GO_0005783; GO_0000324 3862 YKL165C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3863 YKL166C TPK3 cAMP-dependent protein kinase catalytic subunit cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication C IDA; IDA; HDA; IGI; HDA P-body; cytoplasmic stress granule; nucleus; cAMP-dependent protein kinase complex; cytoplasm GO_0000932; GO_0010494; GO_0005634; GO_0005952; GO_0005737 3864 YKL167C MRP49 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 3865 YKL168C KKQ8 Putative serine/threonine protein kinase with unknown cellular role Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 3866 YKL169C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 3867 YKL170W MRPL38 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 3868 YKL171W NNK1 Protein kinase Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3869 YKL172W EBP2 Required for 25S rRNA maturation and 60S ribosomal subunit assembly Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering C IDA; HDA; IDA nucleolus; preribosome, large subunit precursor; nuclear periphery GO_0005730; GO_0030687; GO_0034399 3870 YKL173W SNU114 GTPase component of U5 snRNP involved in mRNA splicing via spliceosome GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 C IDA; IDA; HDA U4/U6 x U5 tri-snRNP complex; U5 snRNP; nucleus GO_0046540; GO_0005682; GO_0005634 3871 YKL174C TPO5 Protein involved in excretion of putrescine and spermidine Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles C HDA; IDA endoplasmic reticulum; Golgi apparatus GO_0005783; GO_0005794 3872 YKL175W ZRT3 Vacuolar membrane zinc transporter Vacuolar membrane zinc transporter; transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 3873 YKL176C LST4 Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface C IDA; IDA; HDA; IDA vacuolar membrane; Lst4-Lst7 complex; cytosol; cytoplasm GO_0005774; GO_1990877; GO_0005829; GO_0005737 3874 YKL177W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3 3875 YKL178C STE3 Receptor for a factor pheromone Receptor for a factor pheromone; couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR C HDA; IDA endoplasmic reticulum; integral component of plasma membrane GO_0005783; GO_0005887 3876 YKL179C COY1 Golgi membrane protein with similarity to mammalian CASP Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function C IGI Golgi membrane GO_0000139 3877 YKL180W RPL17A Ribosomal 60S subunit protein L17A Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA cytosolic large ribosomal subunit; preribosome, large subunit precursor; cytoplasm GO_0022625; GO_0030687; GO_0005737 3878 YKL181W PRS1 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; plays a key role in cell wall integrity (CWI) pathway; one of five related enzymes, which are active as heteromultimeric complexes; missense mutations in human homolog PRPS1 are associated with neuropathic Arts syndrome and Charcot-Marie Tooth (CMTX5) disease C IDA; IDA; IDA ribose phosphate diphosphokinase complex; cytoplasm; cytoplasmic stress granule GO_0002189; GO_0005737; GO_0010494 3879 YKL182W FAS1 Beta subunit of fatty acid synthetase Beta subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities C HDA; IDA; HDA; IDA; IDA mitochondrion; fatty acid synthase complex; cytoplasm; lipid particle; cytosol GO_0005739; GO_0005835; GO_0005737; GO_0005811; GO_0005829 3880 YKL183C-A YKL183C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 3881 YKL183W LOT5 Protein of unknown function Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3882 YKL184W SPE1 Ornithine decarboxylase Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation C HDA cytoplasm GO_0005737 3883 YKL185W ASH1 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate C HDA; IDA; IDA; HDA Rpd3L complex; nucleus; cellular bud; Rpd3L-Expanded complex GO_0033698; GO_0005634; GO_0005933; GO_0070210 3884 YKL186C MTR2 mRNA transport regulator mRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA C IPI; IDA nuclear RNA export factor complex; nuclear pore GO_0042272; GO_0005643 3885 YKL187C FAT3 Protein required for fatty acid uptake Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication C HDA; HDA mitochondrion; cell periphery GO_0005739; GO_0071944 3886 YKL188C PXA2 Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1p Subunit of a heterodimeric peroxisomal ABC transport complex; required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; complex also includes Pxa1p C IDA; IDA peroxisome; integral component of peroxisomal membrane GO_0005777; GO_0005779 3887 YKL189W HYM1 Component of the RAM signaling network Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response C IDA; IDA; IDA; IDA incipient cellular bud site; intracellular; cellular bud; mating projection tip GO_0000131; GO_0005622; GO_0005933; GO_0043332 3888 YKL190W CNB1 Calcineurin B Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress C IDA calcineurin complex GO_0005955 3889 YKL191W DPH2 Protein required for synthesis of diphthamide Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p C HDA cytoplasm GO_0005737 3890 YKL192C ACP1 Mitochondrial matrix acyl carrier protein Mitochondrial matrix acyl carrier protein; involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p C HDA; IPI mitochondrion; mitochondrial iron-sulfur cluster assembly complex GO_0005739; GO_0099128 3891 YKL193C SDS22 Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function C HDA; HDA; IDA nucleus; cytoplasm; protein phosphatase type 1 complex GO_0005634; GO_0005737; GO_0000164 3892 YKL194C MST1 Mitochondrial threonyl-tRNA synthetase Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro C IDA mitochondrion GO_0005739 3893 YKL195W MIA40 Import and assembly protein in mitochondrial intermembrane space Mitochondrial oxidoreductase; involved in mitochondrial intermembrane space import; component of MIA pathway which mediates import and oxidative folding of substrates including small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p via a chaperone-like activity; forms a dimer that binds iron-sulfur cluster in vitro C HDA; IDA; IDA mitochondrion; integral component of mitochondrial inner membrane; mitochondrial intermembrane space GO_0005739; GO_0031305; GO_0005758 3894 YKL196C YKT6 Vesicle membrane protein (v-SNARE) with acyltransferase activity Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus C IDA; HDA; HDA; IDA; IDA; IDA; IDA membrane; cytosol; mitochondrion; SNARE complex; fungal-type vacuole; Golgi apparatus; endosome GO_0016020; GO_0005829; GO_0005739; GO_0031201; GO_0000324; GO_0005794; GO_0005768 3895 YKL197C PEX1 AAA-peroxin AAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis C HDA; HDA; IDA cytosol; peroxisome; peroxisomal membrane GO_0005829; GO_0005777; GO_0005778 3896 YKL198C PTK1 Putative serine/threonine protein kinase Putative serine/threonine protein kinase; regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein; PTK1 has a paralog, PTK2, that arose from the whole genome duplication 3897 YKL201C MNN4 Putative positive regulator of mannosylphosphate transferase Mnn6p Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; coding sequence contains length polymorphisms in different strains; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication 3898 YKL202W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3899 YKL203C TOR2 PIK-related protein kinase and rapamycin target PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication C HDA; IDA; IDA; IPI; IDA; IPI mitochondrion; extrinsic component of cytoplasmic side of plasma membrane; plasma membrane; TORC2 complex; fungal-type vacuole membrane; TORC1 complex GO_0005739; GO_0031234; GO_0005886; GO_0031932; GO_0000329; GO_0031931 3900 YKL204W EAP1 eIF4E-associated protein, competes with eIF4G for binding to eIF4E eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade C IDA; IDA; IPI polysome; cytoplasmic stress granule; mRNA cap binding complex GO_0005844; GO_0010494; GO_0005845 3901 YKL205W LOS1 Nuclear pore protein Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm C HDA; IDA; IDA mitochondrion; nuclear matrix; cytoplasm GO_0005739; GO_0016363; GO_0005737 3902 YKL206C ADD66 Protein involved in 20S proteasome assembly Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly C IDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 3903 YKL207W EMC3 Member of conserved ER transmembrane complex Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 C IDA ER membrane protein complex GO_0072546 3904 YKL208W CBT1 Protein involved in 5' RNA end processing Protein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p C HDA mitochondrion GO_0005739 3905 YKL209C STE6 Plasma membrane ATP-binding cassette (ABC) transporter Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells C IDA; HDA; IDA; IDA mating projection tip; fungal-type vacuole; Golgi apparatus; plasma membrane GO_0043332; GO_0000324; GO_0005794; GO_0005886 3906 YKL210W UBA1 Ubiquitin activating enzyme (E1) Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3907 YKL211C TRP3 Indole-3-glycerol-phosphate synthase Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p C IPI; HDA anthranilate synthase complex; cytoplasm GO_0005950; GO_0005737 3908 YKL212W SAC1 Phosphatidylinositol phosphate (PtdInsP) phosphatase Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism C IDA; IMP; IDA; HDA; HDA; HDA; IDA integral component of Golgi membrane; integral component of endoplasmic reticulum membrane; Golgi medial cisterna; mitochondrial outer membrane; endoplasmic reticulum; mitochondrion; SPOTS complex GO_0030173; GO_0030176; GO_0005797; GO_0005741; GO_0005783; GO_0005739; GO_0035339 3909 YKL213C DOA1 WD-repeat protein involved in ubiquitin-mediated protein degradation WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; facilitates N-terminus-dependent proteolysis of centromeric histone H3 (Cse4p) for faithful chromosome segregation; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3910 YKL214C YRA2 Member of the REF (RNA and export factor binding proteins) family Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus C IDA nucleus GO_0005634 3911 YKL215C OXP1 5-oxoprolinase 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 3912 YKL216W URA1 Dihydroorotate dehydrogenase Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid C IDA; IDA extrinsic component of membrane; cytoplasm GO_0019898; GO_0005737 3913 YKL217W JEN1 Monocarboxylate/proton symporter of the plasma membrane Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose C IDA; HDA; HDA; HDA plasma membrane; mitochondrion; fungal-type vacuole; cell periphery GO_0005886; GO_0005739; GO_0000324; GO_0071944 3914 YKL218C SRY1 3-hydroxyaspartate dehydratase 3-hydroxyaspartate dehydratase; deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity 3915 YKL219W COS9 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins 3916 YKL220C FRE2 Ferric reductase and cupric reductase Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels C IDA; HDA plasma membrane; fungal-type vacuole GO_0005886; GO_0000324 3917 YKL221W MCH2 Protein with similarity to mammalian monocarboxylate permeases Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport 3918 YKL222C YKL222C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine C IDA; HDA; IDA mitotic spindle; ribosome; nucleus GO_0072686; GO_0005840; GO_0005634 3919 YKL223W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3920 YKL224C PAU16 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions C HDA fungal-type vacuole GO_0000324 3921 YKL225W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3922 YKR001C VPS1 Dynamin-like GTPase required for vacuolar sorting Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis C IDA; IDA; HDA; IDA; IDA; HDA; HDA fungal-type vacuole membrane; late endosome; cytosol; actin cortical patch; peroxisome; mitochondrion; mitochondrial outer membrane GO_0000329; GO_0005770; GO_0005829; GO_0030479; GO_0005777; GO_0005739; GO_0005741 3923 YKR002W PAP1 Poly(A) polymerase Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping C IPI; IDA nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex GO_0005654; GO_0005847 3924 YKR003W OSH6 Member of an oxysterol-binding protein family Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication C IDA; IDA; IDA extrinsic component of membrane; cytoplasm; cortical endoplasmic reticulum GO_0019898; GO_0005737; GO_0032541 3925 YKR004C ECM9 Non-essential protein of unknown function Non-essential protein of unknown function 3926 YKR005C YKR005C Putative protein of unknown function Putative protein of unknown function C HDA mitochondrion GO_0005739 3927 YKR006C MRPL13 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 3928 YKR007W MEH1 Subunit of the EGO/GSE complex Component of the EGO and GSE complexes; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification C HDA; IDA; IDA; IDA cytosol; fungal-type vacuole membrane; late endosome membrane; EGO complex GO_0005829; GO_0000329; GO_0031902; GO_0034448 3929 YKR008W RSC4 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p C IDA RSC complex GO_0016586 3930 YKR009C FOX2 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase; multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway 3931 YKR010C TOF2 Protein required for rDNA silencing and mitotic rDNA condensation Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication C IDA; HDA nucleolus; mitochondrion GO_0005730; GO_0005739 3932 YKR011C YKR011C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress C HDA nucleus GO_0005634 3933 YKR012C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 3934 YKR013W PRY2 Sterol binding protein involved in the export of acetylated sterols Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication C HDA; HDA; IDA fungal-type vacuole; endoplasmic reticulum; extracellular region GO_0000324; GO_0005783; GO_0005576 3935 YKR014C YPT52 Endosomal Rab family GTPase Endosomal Rab family GTPase; required for vacuolar protein sorting, endocytosis and multivesicular body (MVB) biogenesis and sorting; required for localization of the CORVET complex to endosomes; involved in autophagy and ionic stress tolerance; similar to Vps21p and Ypt53p; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress C IMP; HDA late endosome; fungal-type vacuole membrane GO_0005770; GO_0000329 3936 YKR015C YKR015C Putative protein of unknown function Putative protein of unknown function 3937 YKR016W MIC60 Component of the MICOS complex Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic60p is also involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; ortholog of mammalian mitofilin C HDA; IDA; IDA; IDA; IDA mitochondrion; mitochondrial crista junction; mitochondrial inner membrane; MICOS complex; mitochondrial crista GO_0005739; GO_0044284; GO_0005743; GO_0061617; GO_0030061 3938 YKR017C HEL1 RING finger ubiquitin ligase (E3) RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) 3939 YKR018C YKR018C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3940 YKR019C IRS4 EH domain-containing protein EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication C IDA; HDA pre-autophagosomal structure; mitochondrion GO_0000407; GO_0005739 3941 YKR020W VPS51 Component of the GARP (Golgi-associated retrograde protein) complex Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p, C IDA; HDA; IPI Golgi apparatus; cytosol; GARP complex GO_0005794; GO_0005829; GO_0000938 3942 YKR021W ALY1 Alpha arrestin, substrate of calcineurin Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA cytosol; late endosome; early endosome; cytoplasm GO_0005829; GO_0005770; GO_0005769; GO_0005737 3943 YKR022C NTR2 Essential protein that forms a dimer with Ntr1p Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly C HDA; IDA; HDA; IDA; HDA endoplasmic reticulum; U2-type post-mRNA release spliceosomal complex; nucleus; spliceosomal complex; cytoplasm GO_0005783; GO_0071008; GO_0005634; GO_0005681; GO_0005737 3944 YKR023W YKR023W Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 3945 YKR024C DBP7 Putative ATP-dependent RNA helicase of the DEAD-box family Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions C IDA nucleolus GO_0005730 3946 YKR025W RPC37 RNA polymerase III subunit C37 RNA polymerase III subunit C37 C IDA DNA-directed RNA polymerase III complex GO_0005666 3947 YKR026C GCN3 Alpha subunit of translation initiation factor eIF2B Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions C IDA; IDA; HDA eukaryotic translation initiation factor 2B complex; guanyl-nucleotide exchange factor complex; cytosol GO_0005851; GO_0032045; GO_0005829 3948 YKR027W BCH2 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication C HDA; IDA; IDA; HDA mitochondrion; trans-Golgi network transport vesicle; exomer complex; clathrin-coated vesicle GO_0005739; GO_0030140; GO_0034044; GO_0030136 3949 YKR028W SAP190 Protein that forms a complex with the Sit4p protein phosphatase Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication C IPI cytoplasm GO_0005737 3950 YKR029C SET3 Defining member of the SET3 histone deacetylase complex Defining member of the SET3 histone deacetylase complex; which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication C HDA; IDA Rpd3L-Expanded complex; Set3 complex GO_0070210; GO_0034967 3951 YKR030W GMH1 Golgi membrane protein of unknown function Golgi membrane protein of unknown function; interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting C IDA integral component of Golgi membrane GO_0030173 3952 YKR031C SPO14 Phospholipase D Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions C IDA; IDA; IDA prospore membrane; nucleus; endosome GO_0005628; GO_0005634; GO_0005768 3953 YKR032W YKR032W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3954 YKR033C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 3955 YKR034W DAL80 Negative regulator of genes in multiple nitrogen degradation pathways Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication C IDA nucleus GO_0005634 3956 YKR035C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A 3957 YKR035W-A DID2 Class E protein of the vacuolar protein-sorting (Vps) pathway Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors C IDA; IDA cytoplasm; late endosome GO_0005737; GO_0005770 3958 YKR036C CAF4 WD40 repeat-containing protein associated with the CCR4-NOT complex WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication C HDA; IDA mitochondrion; CCR4-NOT complex GO_0005739; GO_0030014 3959 YKR037C SPC34 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body C IDA DASH complex GO_0042729 3960 YKR038C KAE1 Highly conserved ATPase of HSP70/DnaK family Highly conserved ATPase of HSP70/DnaK family; essential functional component of the EKC/KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription C HDA; IDA; IDA; HDA nucleus; nuclear chromatin; EKC/KEOPS complex; cytoplasm GO_0005634; GO_0000790; GO_0000408; GO_0005737 3961 YKR039W GAP1 General amino acid permease General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth C IDA; IDA; HDA; IDA; IDA; IMP; IDA plasma membrane; fungal-type vacuole lumen; endoplasmic reticulum; ER to Golgi transport vesicle; integral component of plasma membrane; multivesicular body; endosome GO_0005886; GO_0000328; GO_0005783; GO_0030134; GO_0005887; GO_0005771; GO_0005768 3962 YKR040C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W 3963 YKR041W YKR041W Protein of unknown function Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking C IDA; HDA; IDA mitotic spindle; cytoplasm; nucleus GO_0072686; GO_0005737; GO_0005634 3964 YKR042W UTH1 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; role in mitophagy is disputed; implicated in cell wall biogenesis, the oxidative stress response, life span during starvation, and cell death; SUN family member; UTH1 has a paralog, NCA3, that arose from the whole genome duplication C IDA extrinsic component of mitochondrial inner membrane GO_0031314 3965 YKR043C SHB17 Sedoheptulose bisphosphatase involved in riboneogenesis Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 3966 YKR044W UIP5 Protein of unknown function that interacts with Ulp1p Protein of unknown function that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates C IDA; HDA nuclear envelope; fungal-type vacuole GO_0005635; GO_0000324 3967 YKR045C YKR045C Putative protein of unknown function Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 3968 YKR046C PLN1 Protein of unknown function that localizes to lipid particles Protein of unknown function that localizes to lipid particles; localization suggests a role in lipid metabolism; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange C IDA lipid particle GO_0005811 3969 YKR047W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 3970 YKR048C NAP1 Histone chaperone Histone chaperone; involved in histone exchange by removing and replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; involved in the transport of H2A and H2B histones to the nucleus; required for the regulation of microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress C IDA cytoplasm GO_0005737 3971 YKR049C FMP46 Putative redox protein containing a thioredoxin fold Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3972 YKR050W TRK2 Component of the Trk1p-Trk2p potassium transport system Component of the Trk1p-Trk2p potassium transport system; contributes to K(+) supply and maintenance of plasma-membrane potential; TRK2 has a paralog, TRK1, that arose from the whole genome duplication C HDA cell periphery GO_0071944 3973 YKR051W YKR051W Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 3974 YKR052C MRS4 Iron transporter of the mitochondrial carrier family Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 3975 YKR053C YSR3 Dihydrosphingosine 1-phosphate phosphatase Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 3976 YKR054C DYN1 Cytoplasmic heavy chain dynein Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p C IPI; IDA; IDA; IDA; IDA cytoplasmic dynein complex; cytoplasmic microtubule; spindle pole body; cell cortex; astral microtubule GO_0005868; GO_0005881; GO_0005816; GO_0005938; GO_0000235 3977 YKR055W RHO4 Non-essential small GTPase Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi C IDA; IDA; HDA; HDA; HDA plasma membrane; incipient cellular bud site; cellular bud neck; cell periphery; endoplasmic reticulum GO_0005886; GO_0000131; GO_0005935; GO_0071944; GO_0005783 3978 YKR056W TRM2 tRNA methyltransferase tRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair 3979 YKR057W RPS21A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 3980 YKR058W GLG1 Glycogenin glucosyltransferase Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication 3981 YKR059W TIF1 Translation initiation factor eIF4A Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication C IDA; IGI cytoplasmic stress granule; eukaryotic translation initiation factor 4F complex GO_0010494; GO_0016281 3982 YKR060W UTP30 Putative subunit of U3-containing 90S preribosome complex Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit C HDA; HDA; HDA nucleolus; 90S preribosome; nucleus GO_0005730; GO_0030686; GO_0005634 3983 YKR061W KTR2 Mannosyltransferase involved in N-linked protein glycosylation Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication C IDA Golgi apparatus GO_0005794 3984 YKR062W TFA2 TFIIE small subunit TFIIE small subunit; involved in RNA polymerase II transcription initiation C IDA; IDA transcription factor TFIIE complex; transcriptional preinitiation complex GO_0005673; GO_0097550 3985 YKR063C LAS1 Endonuclease involved in pre-rRNA processing at both ends of ITS2 Protein required for pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability C HDA; IDA; IDA; IPI; IDA mitochondrion; nucleolus; nucleus; preribosome, large subunit precursor; cytoplasm GO_0005739; GO_0005730; GO_0005634; GO_0030687; GO_0005737 3986 YKR064W OAF3 Putative transcriptional repressor with Zn(2)-Cys(6) finger Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress C HDA; HDA; HDA nucleus; cytoplasm; mitochondrion GO_0005634; GO_0005737; GO_0005739 3987 YKR065C PAM17 Constituent of the TIM23 complex Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress C HDA; IDA mitochondrion; presequence translocase-associated import motor GO_0005739; GO_0001405 3988 YKR066C CCP1 Mitochondrial cytochrome-c peroxidase Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress C IDA; HDA mitochondrial intermembrane space; mitochondrion GO_0005758; GO_0005739 3989 YKR067W GPT2 Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen C IDA; IDA; HDA lipid particle; endoplasmic reticulum; cytoplasm GO_0005811; GO_0005783; GO_0005737 3990 YKR068C BET3 Core component of transport protein particle (TRAPP) complexes I-III Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles C IDA; IDA; IDA; HDA; IDA TRAPPI protein complex; TRAPPII protein complex; TRAPPIII protein complex; cytosol; cis-Golgi network membrane GO_1990070; GO_1990071; GO_1990072; GO_0005829; GO_0033106 3991 YKR069W MET1 S-adenosyl-L-methionine uroporphyrinogen III transmethylase S-adenosyl-L-methionine uroporphyrinogen III transmethylase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis C HDA nucleus GO_0005634 3992 YKR070W YKR070W Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 3993 YKR071C DRE2 Component of the cytosolic Fe-S protein assembly (CIA) machinery Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA cytoplasm; cytosol; mitochondrial intermembrane space GO_0005737; GO_0005829; GO_0005758 3994 YKR072C SIS2 Negative regulatory subunit of protein phosphatase 1 (Ppz1p) Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication C IDA; IDA; IPI; IDA phosphopantothenoylcysteine decarboxylase complex; CoA-synthesizing protein complex; cytoplasm; nucleus GO_0071513; GO_1990143; GO_0005737; GO_0005634 3995 YKR073C YKR073C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 3996 YKR074W AIM29 Protein of unknown function Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss C HDA cytoplasm GO_0005737 3997 YKR075C YKR075C Protein of unknown function Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 3998 YKR075W-A Dubious open reading frame unlikely to encode a protein; completely overlaps the uncharacterized gene YKR075C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 3999 YKR076W ECM4 S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR) Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 4000 YKR077W MSA2 Putative transcriptional activator Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4001 YKR078W YKR078W Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4002 YKR079C TRZ1 tRNA 3'-end processing endonuclease tRNase Z tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 C IDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 4003 YKR080W MTD1 NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline C IDA cytosol GO_0005829 4004 YKR081C RPF2 Essential protein involved in rRNA maturation and ribosomal assembly Essential protein involved in rRNA maturation and ribosomal assembly; involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles C IPI; HDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 4005 YKR082W NUP133 Subunit of Nup84p subcomplex of nuclear pore complex (NPC) Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133 C IDA; IDA; IDA; IDA cytosol; nucleus; nuclear pore outer ring; nuclear pore GO_0005829; GO_0005634; GO_0031080; GO_0005643 4006 YKR083C DAD2 Essential subunit of the Dam1 complex (aka DASH complex) Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis C IDA DASH complex GO_0042729 4007 YKR084C HBS1 GTPase with similarity to translation release factors GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication C HDA; IDA; HDA cytosol; Dom34-Hbs1 complex; cytoplasm GO_0005829; GO_1990533; GO_0005737 4008 YKR085C MRPL20 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 4009 YKR086W PRP16 DEAH-box RNA helicase involved in second catalytic step of splicing a DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa C IDA U2-type catalytic step 2 spliceosome GO_0071007 4010 YKR087C OMA1 Metalloendopeptidase of the mitochondrial inner membrane Metalloendopeptidase of the mitochondrial inner membrane; important for adaptive responses to various homeostatic insults and preservation of normal mitochondrial function under damage-eliciting conditions; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 4011 YKR088C TVP38 Integral membrane protein Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern C HDA; IDA cytoplasm; integral component of Golgi membrane GO_0005737; GO_0030173 4012 YKR089C TGL4 Multifunctional lipase/hydrolase/phospholipase Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication C IDA lipid particle GO_0005811 4013 YKR090W PXL1 Protein that localizes to sites of polarized growth Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress C IDA; HDA; IDA; HDA; IDA; IDA; IDA mating projection tip; cellular bud; incipient cellular bud site; cytoplasm; cellular bud neck; cellular bud tip; site of polarized growth GO_0043332; GO_0005933; GO_0000131; GO_0005737; GO_0005935; GO_0005934; GO_0030427 4014 YKR091W SRL3 GTB motif (G1/S transcription factor binding) containing protein GTB motif (G1/S transcription factor binding) containing protein; binds SBF-regulated promoters in hydroxyurea-treated cells; when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate; SRL3 has a paralog, WHI5, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4015 YKR092C SRP40 Nucleolar serine-rich protein Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 C IDA nucleolus GO_0005730 4016 YKR093W PTR2 Integral membrane peptide transporter Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p C HDA; HDA; IDA cell periphery; fungal-type vacuole; plasma membrane GO_0071944; GO_0000324; GO_0005886 4017 YKR094C RPL40B Ubiquitin-ribosomal 60S subunit protein L40B fusion protein Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication C IDA; HDA cytosolic large ribosomal subunit; mitochondrion GO_0022625; GO_0005739 4018 YKR095W MLP1 Myosin-like protein associated with the nuclear envelope Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication C IPI; IDA; IDA; IDA; IDA ribonucleoprotein complex; spindle pole body; nuclear pore nuclear basket; nuclear envelope; nucleoplasm GO_1990904; GO_0005816; GO_0044615; GO_0005635; GO_0005654 4019 YKR095W-A PCC1 Component of the EKC/KEOPS protein complex Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; other complex members are Kae1p, Gon7p, Bud32p, and Cgi121p C IDA; IDA EKC/KEOPS complex; nuclear chromatin GO_0000408; GO_0000790 4020 YKR096W ESL2 hEST1A/B (SMG5/6)-like protein Protein of unknown function; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain; ESL2 has a paralog, ESL1, that arose from the whole genome duplication C HDA; HDA; HDA; IDA ribosome; nucleus; cytoplasm; peroxisomal importomer complex GO_0005840; GO_0005634; GO_0005737; GO_1990429 4021 YKR097W PCK1 Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol C IDA cytosol GO_0005829 4022 YKR098C UBP11 Ubiquitin-specific protease Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication 4023 YKR099W BAS1 Myb-related transcription factor Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes C IDA nucleus GO_0005634 4024 YKR100C SKG1 Transmembrane protein with a role in cell wall polymer composition Transmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication C HDA; IDA; IDA cellular bud; cellular bud tip; incipient cellular bud site GO_0005933; GO_0005934; GO_0000131 4025 YKR101W SIR1 Protein involved in silencing at mating-type loci HML and HMR Protein involved in silencing at mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin C IDA chromatin silencing complex GO_0005677 4026 YKR102W FLO10 Member of the FLO family of cell wall flocculation proteins Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth C HDA cell periphery GO_0071944 4027 YKR103W NFT1 Putative transporter of the MRP subfamily Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein C IDA fungal-type vacuole membrane GO_0000329 4028 YKR104W YKR104W Putative transporter of the MRP subfamily Putative transporter of the MRP subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein 4029 YKR105C VBA5 Plasma membrane protein of the Major Facilitator Superfamily (MFS) Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication C IDA plasma membrane GO_0005886 4030 YKR106W GEX2 Proton:glutathione antiporter Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; expressed at a very low level; potential role in resistance to oxidative stress and modulation of the PKA pathway; GEX2 has a paralog, GEX1, that arose from a segmental duplication C IDA; HDA; IDA vacuolar membrane; cell periphery; plasma membrane GO_0005774; GO_0071944; GO_0005886 4031 YLL001W DNM1 Dynamin-related GTPase involved in mitochondrial organization Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance C HDA; IDA; IDA; IDA mitochondrion; cytosol; mitochondrial outer membrane; peroxisome GO_0005739; GO_0005829; GO_0005741; GO_0005777 4032 YLL002W RTT109 Histone acetyltransferase Histone acetyltransferase; critical for cell survival in the presence of DNA damage during S phase; prevents hyper-amplification of rDNA; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p C IDA nucleus GO_0005634 4033 YLL003W SFI1 Centrin (Cdc31p)-binding protein required for SPB duplication Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C C IDA half bridge of spindle pole body GO_0005825 4034 YLL004W ORC3 Subunit of the origin recognition complex (ORC) Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing C IDA; IMP; IDA nuclear pre-replicative complex; nuclear origin of replication recognition complex; DNA replication preinitiation complex GO_0005656; GO_0005664; GO_0031261 4035 YLL005C SPO75 Meiosis-specific protein of unknown function Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis 4036 YLL006W MMM1 ER integral membrane protein, ERMES complex subunit ER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase C IDA; IDA; HDA; HDA integral component of endoplasmic reticulum membrane; ERMES complex; mitochondrial outer membrane; mitochondrion GO_0030176; GO_0032865; GO_0005741; GO_0005739 4037 YLL006W-A YLL006W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 4038 YLL007C LMO1 Homolog of mammalian ELMO (Engulfment and celL MOtility) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene C IDA; IDA; HDA plasma membrane; mitochondrion; cytoplasm GO_0005886; GO_0005739; GO_0005737 4039 YLL008W DRS1 Nucleolar DEAD-box protein required for ribosome assembly and function Nucleolar DEAD-box protein required for ribosome assembly and function; including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles C HDA; HDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 4040 YLL009C COX17 Copper metallochaperone that transfers copper to Sco1p and Cox11p Copper metallochaperone that transfers copper to Sco1p and Cox11p; eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs C IDA; HDA; IDA cytosol; mitochondrion; mitochondrial intermembrane space GO_0005829; GO_0005739; GO_0005758 4041 YLL010C PSR1 Plasma membrane associated protein phosphatase Plasma membrane associated protein phosphatase; involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR1 has a paralog, PSR2, that arose from the whole genome duplication C IDA; HDA plasma membrane; cytosol GO_0005886; GO_0005829 4042 YLL011W SOF1 Protein required for biogenesis of 40S (small) ribosomal subunit Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene C HDA; IDA; IDA 90S preribosome; nucleolus; small-subunit processome GO_0030686; GO_0005730; GO_0032040 4043 YLL012W YEH1 Steryl ester hydrolase Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication C IDA; HDA integral component of membrane; lipid particle GO_0016021; GO_0005811 4044 YLL013C PUF3 Protein of the mitochondrial outer surface Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins C IDA; IDA cytoplasm; cytoplasmic side of mitochondrial outer membrane GO_0005737; GO_0032473 4045 YLL014W EMC6 Member of conserved ER transmembrane complex Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 C HDA; IDA endoplasmic reticulum; ER membrane protein complex GO_0005783; GO_0072546 4046 YLL015W BPT1 ABC type transmembrane transporter of MRP/CFTR family ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 4047 YLL018C DPS1 Aspartyl-tRNA synthetase, primarily cytoplasmic Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation; shares five highly conserved amino acids with human that when mutated cause leukoencephalopathy characterized by hypomyelination with brain stem and spinal cord involvement and leg spasticity (HBSL) C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4048 YLL018C-A COX19 Protein required for cytochrome c oxidase assembly Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs C IDA; IDA mitochondrial intermembrane space; cytosol GO_0005758; GO_0005829 4049 YLL019C KNS1 Protein kinase involved in negative regulation of PolIII transcription Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4050 YLL019W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene KNS1 4051 YLL020C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 4052 YLL021W SPA2 Component of the polarisome Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA; IDA mating projection tip; cellular bud tip; polarisome; incipient cellular bud site; cellular bud neck; cell cortex GO_0043332; GO_0005934; GO_0000133; GO_0000131; GO_0005935; GO_0005938 4053 YLL022C HIF1 Non-essential component of the HAT-B histone acetyltransferase complex Non-essential component of the HAT-B histone acetyltransferase complex; localized to the nucleus; has a role in telomeric silencing; other members are Hat1p and Hat2p C IMP; IDA histone acetyltransferase complex; nucleus GO_0000123; GO_0005634 4054 YLL023C POM33 Transmembrane nucleoporin Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA endoplasmic reticulum; ribosome; nuclear pore GO_0005783; GO_0005840; GO_0005643 4055 YLL024C SSA2 HSP70 family ATP-binding protein ATP-binding protein; involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes C IDA; IDA; HDA; IDA; IDA; IPI; IDA; IDA polysome; mitochondrion; plasma membrane; cytosol; fungal-type vacuole membrane; chaperonin-containing T-complex; cytoplasm; fungal-type cell wall GO_0005844; GO_0005739; GO_0005886; GO_0005829; GO_0000329; GO_0005832; GO_0005737; GO_0009277 4056 YLL025W PAU17 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene C HDA fungal-type vacuole GO_0000324 4057 YLL026W HSP104 Disaggregase Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation C IDA; IDA; IDA; HDA TRC complex; nucleus; nuclear periphery; cytoplasm GO_0072380; GO_0005634; GO_0034399; GO_0005737 4058 YLL027W ISA1 Protein required for maturation of mitochondrial [4Fe-4S] proteins Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins C IDA mitochondrial matrix GO_0005759 4059 YLL028W TPO1 Polyamine transporter of the major facilitator superfamily Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane C HDA; IMP; IDA; IDA cell periphery; fungal-type vacuole membrane; plasma membrane; cellular bud membrane GO_0071944; GO_0000329; GO_0005886; GO_0033101 4060 YLL029W FRA1 Protein involved in negative regulation of iron regulon transcription Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation C IDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 4061 YLL030C RRT7 Putative protein of unknown function Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 4062 YLL031C GPI13 ER membrane localized phosphoryltransferase ER membrane localized phosphoryltransferase; adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein C HDA endoplasmic reticulum GO_0005783 4063 YLL032C YLL032C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene C HDA; HDA cytoplasm; ribosome GO_0005737; GO_0005840 4064 YLL033W IRC19 Protein of unknown function Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci 4065 YLL034C RIX7 Putative ATPase of the AAA family Putative ATPase of the AAA family; required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions C IDA; HDA; IDA preribosome, large subunit precursor; nucleolus; nucleus GO_0030687; GO_0005730; GO_0005634 4066 YLL035W GRC3 Polynucleotide kinase present on rDNA Polynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated C IPI nucleolar chromatin GO_0030874 4067 YLL036C PRP19 Splicing factor associated with the spliceosome Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia C HDA; IDA; IDA; IDA; IDA; HDA mitochondrion; nucleus; cytosol; U2-type catalytic step 1 spliceosome; Prp19 complex; cytoplasm GO_0005739; GO_0005634; GO_0005829; GO_0071006; GO_0000974; GO_0005737 4068 YLL037W Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor 4069 YLL038C ENT4 Protein of unknown function Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome C HDA cytosol GO_0005829 4070 YLL039C UBI4 Ubiquitin Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress C IDA peroxisomal importomer complex GO_1990429 4071 YLL040C VPS13 Protein involved in prospore membrane morphogenesis Protein involved in prospore membrane morphogenesis; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulatoin, and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively C IDA; IDA; IDA; HDA; HDA; IDA; HDA nucleus-vacuole junction; vacuole-mitochondrion membrane contact site; extrinsic component of membrane; mitochondrion; prospore membrane; organelle membrane contact site; endosome GO_0071561; GO_1990816; GO_0019898; GO_0005739; GO_0005628; GO_0044232; GO_0005768 4072 YLL041C SDH2 Iron-sulfur protein subunit of succinate dehydrogenase Iron-sulfur protein subunit of succinate dehydrogenase; the complex couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; other members are Sdh1p, Sdh3p, and Sdh4p C HDA; IDA; IDA; IDA mitochondrion; fungal-type vacuole membrane; mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone); mitochondrial membrane GO_0005739; GO_0000329; GO_0005749; GO_0031966 4073 YLL042C ATG10 Conserved E2-like conjugating enzyme Conserved E2-like conjugating enzyme; mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy 4074 YLL043W FPS1 Aquaglyceroporin, plasma membrane channel Aquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; role in mediating passive diffusion of glycerol is key factor in maintenance of redox balance; member of major intrinsic protein (MIP) family; phosphorylated by Hog1p MAPK under acetate stress; deletion improves xylose fermentation C HDA; HDA; IDA; HDA fungal-type vacuole; cell periphery; plasma membrane; cytoplasm GO_0000324; GO_0071944; GO_0005886; GO_0005737 4075 YLL044W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant 4076 YLL045C RPL8B Ribosomal 60S subunit protein L8B Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4077 YLL046C RNP1 Ribonucleoprotein that contains two RNA recognition motifs (RRM) Ribonucleoprotein that contains two RNA recognition motifs (RRM); RNP1 has a paralog, SBP1, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 4078 YLL047W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 4079 YLL048C YBT1 Transporter of the ATP-binding cassette (ABC) family Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication C IDA; HDA fungal-type vacuole; endoplasmic reticulum GO_0000324; GO_0005783 4080 YLL049W LDB18 Component of the dynactin complex Component of the dynactin complex; dynactin is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 C IDA; IDA; IDA; IDA spindle pole body; astral microtubule; cell cortex; dynactin complex GO_0005816; GO_0000235; GO_0005938; GO_0005869 4081 YLL050C COF1 Cofilin, involved in pH-dependent actin filament depolarization Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes C IDA; HDA actin cortical patch; plasma membrane GO_0030479; GO_0005886 4082 YLL051C FRE6 Putative ferric reductase with similarity to Fre2p Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels C IDA; HDA fungal-type vacuole membrane; fungal-type vacuole GO_0000329; GO_0000324 4083 YLL052C AQY2 Water channel that mediates water transport across cell membranes Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains C HDA; IDA; IDA cytosol; endoplasmic reticulum membrane; plasma membrane GO_0005829; GO_0005789; GO_0005886 4084 YLL053C YLL053C Putative protein Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin 4085 YLL054C YLL054C Putative protein of unknown function with similarity to Pip2p Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene 4086 YLL055W YCT1 High-affinity cysteine-specific transporter High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene C HDA; HDA cell periphery; endoplasmic reticulum GO_0071944; GO_0005783 4087 YLL056C YLL056C Putative protein of unknown function Putative protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin 4088 YLL057C JLP1 Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase; involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation 4089 YLL058W YLL058W Putative protein of unknown function with similarity to Str2p Putative protein of unknown function with similarity to Str2p; Str2p is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene 4090 YLL059C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4091 YLL060C GTT2 Glutathione S-transferase capable of homodimerization Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 4092 YLL061W MMP1 High-affinity S-methylmethionine permease High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p C HDA; IMP cell periphery; plasma membrane GO_0071944; GO_0005886 4093 YLL062C MHT1 S-methylmethionine-homocysteine methyltransferase S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio C HDA cytoplasm GO_0005737 4094 YLL063C AYT1 Acetyltransferase Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis 4095 YLL064C PAU18 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau6p 4096 YLL065W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps chromosome XII telomeric element core sequences 4097 YLL066C YLL066C Putative Y' element ATP-dependent helicase Putative Y' element ATP-dependent helicase; YLL066C is not an essential gene 4098 YLL066W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YLL066C 4099 YLL066W-B YLL066W-B Putative protein of unknown function Putative protein of unknown function; overexpression causes a cell cycle delay or arrest 4100 YLL067C YLL067C Putative Y' element ATP-dependent helicase Putative Y' element ATP-dependent helicase 4101 YLL067W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps YLL067C 4102 YLR001C YLR001C Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated C HDA; HDA; HDA fungal-type vacuole; fungal-type vacuole membrane; mitochondrion GO_0000324; GO_0000329; GO_0005739 4103 YLR002C NOC3 Subunit of a nuclear complex with Noc2p and pre-replicative complexes Subunit of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing C IDA; IPI; IDA; IDA; IDA; HDA nucleolus; Noc2p-Noc3p complex; nuclear pre-replicative complex; nucleus; nucleoplasm; preribosome, large subunit precursor GO_0005730; GO_0030691; GO_0005656; GO_0005634; GO_0005654; GO_0030687 4104 YLR003C CMS1 Putative subunit of the 90S preribosome processome complex Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA; HDA nucleus; 90S preribosome; cytoplasm GO_0005634; GO_0030686; GO_0005737 4105 YLR004C THI73 Putative plasma membrane permease Putative plasma membrane permease; proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs C HDA; IDA cell periphery; endoplasmic reticulum GO_0071944; GO_0005783 4106 YLR005W SSL1 Subunit of the core form of RNA polymerase transcription factor TFIIH Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p C IDA; IDA; IDA core TFIIH complex; nucleotide-excision repair factor 3 complex; holo TFIIH complex GO_0000439; GO_0000112; GO_0005675 4107 YLR006C SSK1 Cytoplasmic phosphorelay intermediate osmosensor and regulator Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate C IDA cytoplasm GO_0005737 4108 YLR007W NSE1 Component of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair C IDA; IDA nucleus; Smc5-Smc6 complex GO_0005634; GO_0030915 4109 YLR008C PAM18 Subunit of the import motor (PAM complex) Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane presequence translocase complex; presequence translocase-associated import motor GO_0005739; GO_0005744; GO_0001405 4110 YLR009W RLP24 Essential protein required for ribosomal large subunit biogenesis Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp C HDA; IDA preribosome, large subunit precursor; nucleolus GO_0030687; GO_0005730 4111 YLR010C TEN1 Protein that regulates telomeric length Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p; similar to human Ten1 which is critical for the telomeric function of the CST (Cdc13p-Stn1p-Ten1p) complex C IPI CST complex GO_1990879 4112 YLR011W LOT6 FMN-dependent NAD(P)H:quinone reductase FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions C HDA; IDA; IDA cytoplasm; cytosol; nucleus GO_0005737; GO_0005829; GO_0005634 4113 YLR012C YLR012C Putative protein of unknown function Putative protein of unknown function; YLR012C is not an essential gene 4114 YLR013W GAT3 Protein containing GATA family zinc finger motifs Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT4, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants 4115 YLR014C PPR1 Zinc finger transcription factor Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p 4116 YLR015W BRE2 Subunit of COMPASS (Set1C) complex Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L C IPI Set1C/COMPASS complex GO_0048188 4117 YLR016C PML1 Subunit of the RES complex Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p C HDA; IDA; HDA cytoplasm; RES complex; nucleus GO_0005737; GO_0070274; GO_0005634 4118 YLR017W MEU1 Methylthioadenosine phosphorylase (MTAP) Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression C IDA cytoplasm GO_0005737 4119 YLR018C POM34 Subunit of the transmembrane ring of the nuclear pore complex (NPC) Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication C IDA; IDA; IDA; HDA integral component of nuclear outer membrane; nuclear pore; nuclear pore transmembrane ring; nuclear periphery GO_0031309; GO_0005643; GO_0070762; GO_0034399 4120 YLR019W PSR2 Plasma membrane phosphatase Plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR2 has a paralog, PSR1, that arose from the whole genome duplication C HDA cytosol GO_0005829 4121 YLR020C YEH2 Steryl ester hydrolase Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication C IDA; HDA; IDA plasma membrane; cell periphery; integral component of membrane GO_0005886; GO_0071944; GO_0016021 4122 YLR021W IRC25 Component of a heterodimeric Poc4p-Irc25p chaperone Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions C IDA intracellular GO_0005622 4123 YLR022C SDO1 Guanine nucleotide exchange factor (GEF) for Ria1p Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes C IDA; HDA; HDA; IDA polysome; nucleus; cytoplasm; preribosome, large subunit precursor GO_0005844; GO_0005634; GO_0005737; GO_0030687 4124 YLR023C IZH3 Membrane protein involved in zinc ion homeostasis Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity C HDA endoplasmic reticulum GO_0005783 4125 YLR024C UBR2 Cytoplasmic ubiquitin-protein ligase (E3) Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex C IPI; HDA MUB1-RAD6-UBR2 ubiquitin ligase complex; cytoplasm GO_1990304; GO_0005737 4126 YLR025W SNF7 One of four subunits of the ESCRT-III complex One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III C IDA; IDA; IDA ESCRT III complex; cytoplasm; plasma membrane GO_0000815; GO_0005737; GO_0005886 4127 YLR026C SED5 cis-Golgi t-SNARE syntaxin cis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins C IDA; IDA; IDA integral component of membrane; cis-Golgi network; SNARE complex GO_0016021; GO_0005801; GO_0031201 4128 YLR027C AAT2 Cytosolic aspartate aminotransferase involved in nitrogen metabolism Cytosolic aspartate aminotransferase involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells C IDA; IDA cytosol; peroxisome GO_0005829; GO_0005777 4129 YLR028C ADE16 Enzyme of 'de novo' purine biosynthesis Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine C IDA cytosol GO_0005829 4130 YLR029C RPL15A Ribosomal 60S subunit protein L15A Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4131 YLR030W YLR030W Putative protein of unknown function Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W 4132 YLR031W YLR031W Putative protein of unknown function Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W; YLR031W has a paralog, YMR124W, that arose from the whole genome duplication 4133 YLR032W RAD5 DNA helicase/Ubiquitin ligase DNA helicase/Ubiquitin ligase; involved in error-free branch of DNA damage tolerance (DDT) pathway; proposed to promote replication fork regression during postreplication repair by template switching; stimulates synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells C IDA; IDA; HDA; HDA chromosome, telomeric region; nuclear chromatin; cytoplasm; nucleus GO_0000781; GO_0000790; GO_0005737; GO_0005634 4134 YLR033W RSC58 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance C IDA RSC complex GO_0016586 4135 YLR034C SMF3 Putative divalent metal ion transporter involved in iron homeostasis Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress C HDA fungal-type vacuole membrane GO_0000329 4136 YLR035C MLH2 Protein involved in mismatch repair and meiotic recombination Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p C IPI; IPI MutLbeta complex; nucleus GO_0032390; GO_0005634 4137 YLR035C-A YLR035C-A Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); YLR035C-A is part of a mutant retrotransposon C IDA nucleus GO_0005634 4138 YLR036C YLR036C Putative protein predicted to have transmembrane domains Putative protein predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein 4139 YLR037C PAU23 Cell wall mannoprotein Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth C HDA fungal-type vacuole GO_0000324 4140 YLR038C COX12 Subunit VIb of cytochrome c oxidase Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV C HDA; IPI; IDA mitochondrion; mitochondrial respiratory chain complex IV; mitochondrial intermembrane space GO_0005739; GO_0005751; GO_0005758 4141 YLR039C RIC1 Protein involved in retrograde transport to the cis-Golgi network Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes C IDA; IDA; IDA; HDA; IPI Golgi membrane; guanyl-nucleotide exchange factor complex; cis-Golgi network; cytosol; RIC1-RGP1 guanyl-nucleotide exchange factor complex GO_0000139; GO_0032045; GO_0005801; GO_0005829; GO_0034066 4142 YLR040C AFB1 MATalpha-specific a-factor blocker MATalpha-specific a-factor blocker; contributes to mating efficiency under certain conditions; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W C HDA; IDA cell periphery; fungal-type cell wall GO_0071944; GO_0009277 4143 YLR041W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C 4144 YLR042C YLR042C Cell wall protein of unknown function Cell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLL042C is not an essential gene C HDA; IDA; HDA cell periphery; fungal-type cell wall; fungal-type vacuole GO_0071944; GO_0009277; GO_0000324 4145 YLR043C TRX1 Cytoplasmic thioredoxin isoenzyme Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; IPI mitochondrion; mitochondrial intermembrane space; cytosol; nucleus; fungal-type vacuole GO_0005739; GO_0005758; GO_0005829; GO_0005634; GO_0000324 4146 YLR044C PDC1 Major of three pyruvate decarboxylase isozymes Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo C HDA; IDA; IDA cytoplasm; nucleus; cytosol GO_0005737; GO_0005634; GO_0005829 4147 YLR045C STU2 Microtubule-associated protein (MAP) of the XMAP215/Dis1 family Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p C IDA; IDA; IDA; IDA; IDA cell cortex; kinetochore; spindle microtubule; spindle pole body; condensed nuclear chromosome kinetochore GO_0005938; GO_0000776; GO_0005876; GO_0005816; GO_0000778 4148 YLR046C YLR046C Putative membrane protein Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YLR046C has a paralog, RTA1, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 4149 YLR047C FRE8 Protein with sequence similarity to iron/copper reductases Protein with sequence similarity to iron/copper reductases; involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p C HDA; HDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 4150 YLR048W RPS0B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 C IDA cytosolic small ribosomal subunit GO_0022627 4151 YLR049C MLO50 Putative protein of unknown function Putative protein of unknown function 4152 YLR050C YLR050C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene C HDA endoplasmic reticulum GO_0005783 4153 YLR051C FCF2 Nucleolar protein involved in the early steps of 35S rRNA processing Nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon; essential gene C HDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 4154 YLR052W IES3 Subunit of the INO80 chromatin remodeling complex Subunit of the INO80 chromatin remodeling complex C HDA; IDA nucleus; Ino80 complex GO_0005634; GO_0031011 4155 YLR053C YLR053C Putative protein of unknown function Putative protein of unknown function 4156 YLR054C OSW2 Protein of unknown function reputedly involved in spore wall assembly Protein of unknown function reputedly involved in spore wall assembly C HDA; IDA cytoplasm; prospore membrane GO_0005737; GO_0005628 4157 YLR055C SPT8 Subunit of the SAGA transcriptional regulatory complex Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters C HDA; IDA nucleus; SAGA complex GO_0005634; GO_0000124 4158 YLR056W ERG3 C-5 sterol desaturase C-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase C HDA; IDA endoplasmic reticulum; endoplasmic reticulum lumen GO_0005783; GO_0005788 4159 YLR057W MNL2 Putative mannosidase involved in ER-associated protein degradation Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene C IDA endoplasmic reticulum GO_0005783 4160 YLR058C SHM2 Cytosolic serine hydroxymethyltransferase Cytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis C IDA; HDA; HDA cytoplasm; plasma membrane; mating projection tip GO_0005737; GO_0005886; GO_0043332 4161 YLR059C REX2 3'-5' RNA exonuclease 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease C HDA mitochondrion GO_0005739 4162 YLR060W FRS1 Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control C IDA; HDA phenylalanine-tRNA ligase complex; cytoplasm GO_0009328; GO_0005737 4163 YLR061W RPL22A Ribosomal 60S subunit protein L22A Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4164 YLR062C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay 4165 YLR063W BMT6 Methyltransferase required for m3U2843 methylation of the 25S rRNA Methyltransferase required for m3U2843 methylation of the 25S rRNA; S-adenosylmethionine-dependent; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene C HDA cytoplasm GO_0005737 4166 YLR064W PER33 Protein that localizes to the endoplasmic reticulum Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress C IDA; HDA nuclear pore; endoplasmic reticulum GO_0005643; GO_0005783 4167 YLR065C SND2 Protein involved in SRP-independent targeting of substrates to the ER Protein proposed to be involved in vacuolar functions; putative role in secretory protein quality control; mutant shows defect in CPY processing; YLR065C is not an essential gene C HDA endoplasmic reticulum GO_0005783 4168 YLR066W SPC3 Subunit of signal peptidase complex Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p C IDA; HDA signal peptidase complex; endoplasmic reticulum GO_0005787; GO_0005783 4169 YLR067C PET309 Specific translational activator for the COX1 mRNA Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs) C HDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0031314 4170 YLR068W FYV7 Essential protein required for maturation of 18S rRNA Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin C HDA; HDA nucleus; nucleolus GO_0005634; GO_0005730 4171 YLR069C MEF1 Mitochondrial elongation factor involved in translational elongation Mitochondrial elongation factor involved in translational elongation C HDA; HDA cytosol; mitochondrion GO_0005829; GO_0005739 4172 YLR070C XYL2 Xylitol dehydrogenase Xylitol dehydrogenase; converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect 4173 YLR071C RGR1 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation C IDA core mediator complex GO_0070847 4174 YLR072W LAM6 Sterol transporter that transfers sterols between membranes Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; not an esssential gene; YLR072W has a paralog, YFL042C, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; HDA ER-mitochondrion membrane contact site; nucleus-vacuole junction; cytoplasm; vacuole-mitochondrion membrane contact site; mitochondrion GO_0044233; GO_0071561; GO_0005737; GO_1990816; GO_0005739 4175 YLR073C RFU1 Protein that inhibits Doa4p deubiquitinating activity Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes C IDA endosome GO_0005768 4176 YLR074C BUD20 C2H2-type zinc finger protein required for ribosome assembly C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593 C IDA; IDA; HDA nucleus; cytoplasm; preribosome, large subunit precursor GO_0005634; GO_0005737; GO_0030687 4177 YLR075W RPL10 Ribosomal 60S subunit protein L10 Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects C IDA cytosolic large ribosomal subunit GO_0022625 4178 YLR076C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 4179 YLR077W FMP25 Protein required for assembly of respiratory complex III Protein required for assembly of respiratory complex III; mitochondrial inner membrane protein; required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 4180 YLR078C BOS1 v-SNARE (vesicle specific SNAP receptor) v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi C IDA; IDA; IDA; IDA ER to Golgi transport vesicle membrane; SNARE complex; integral component of membrane; endoplasmic reticulum membrane GO_0012507; GO_0031201; GO_0016021; GO_0005789 4181 YLR079W SIC1 Cyclin-dependent kinase inhibitor (CKI) Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 4182 YLR080W EMP46 Integral membrane component of ER-derived COPII-coated vesicles Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication C HDA; IDA; IPI endoplasmic reticulum; Golgi membrane; ER to Golgi transport vesicle GO_0005783; GO_0000139; GO_0030134 4183 YLR081W GAL2 Galactose permease Galactose permease; required for utilization of galactose; also able to transport glucose C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 4184 YLR082C SRL2 Protein of unknown function Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4185 YLR083C EMP70 Protein with a role in cellular adhesion and filamentous growth Protein with a role in cellular adhesion and filamentous growth; also endosome-to-vacuole sorting; similar to Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments; EMP70 has a paralog, TMN2, that arose from the whole genome duplication C IDA; IDA fungal-type vacuole membrane; endosome GO_0000329; GO_0005768 4186 YLR084C RAX2 N-glycosylated protein N-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p C HDA; HDA; IDA; HDA mitochondrion; cellular bud neck; cellular bud scar; endoplasmic reticulum GO_0005739; GO_0005935; GO_0005621; GO_0005783 4187 YLR085C ARP6 Actin-related protein that binds nucleosomes Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A C IDA; IDA; IDA nuclear periphery; Swr1 complex; cytoplasm GO_0034399; GO_0000812; GO_0005737 4188 YLR086W SMC4 Subunit of the condensin complex Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate C IDA; HDA nuclear condensin complex; nucleus GO_0000799; GO_0005634 4189 YLR087C CSF1 Protein required for fermentation at low temperature Protein required for fermentation at low temperature; plays a role in the maturation of secretory proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 4190 YLR088W GAA1 Subunit of the GPI:protein transamidase complex Subunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER C IDA; HDA GPI-anchor transamidase complex; endoplasmic reticulum GO_0042765; GO_0005783 4191 YLR089C ALT1 Alanine transaminase (glutamic pyruvic transaminase) Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive C IDA mitochondrion GO_0005739 4192 YLR090W XDJ1 Chaperone with a role in facilitating mitochondrial protein import Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; HDA; HDA integral component of mitochondrial outer membrane; mitochondrion; nucleus GO_0031307; GO_0005739; GO_0005634 4193 YLR091W GEP5 Protein of unknown function Protein of unknown function; required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine C HDA mitochondrion GO_0005739 4194 YLR092W SUL2 High affinity sulfate permease High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates C HDA plasma membrane GO_0005886 4195 YLR093C NYV1 v-SNARE component of the vacuolar SNARE complex v-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane C IDA; HDA SNARE complex; fungal-type vacuole membrane GO_0031201; GO_0000329 4196 YLR094C GIS3 Protein of unknown function Protein of unknown function C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4197 YLR095C IOC2 Subunit of the Isw1b complex Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p C IDA Isw1b complex GO_0036437 4198 YLR096W KIN2 Serine/threonine protein kinase involved in regulation of exocytosis Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN2 has a paralog, KIN1, that arose from the whole genome duplication C IDA plasma membrane GO_0005886 4199 YLR097C HRT3 Putative SCF-ubiquitin ligase F-box protein Putative SCF-ubiquitin ligase F-box protein; based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies C IDA SCF ubiquitin ligase complex GO_0019005 4200 YLR098C CHA4 DNA binding transcriptional activator DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain C IPI nucleus GO_0005634 4201 YLR099C ICT1 Lysophosphatidic acid acyltransferase Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication 4202 YLR099W-A MIM2 Mitochondrial protein required for outer membrane protein import Mitochondrial protein required for outer membrane protein import; involved in import of the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and Fzo1p; component of a large protein complex in the outer membrane that includes Mim1p; not essential in W303 strain background C IDA integral component of mitochondrial outer membrane GO_0031307 4203 YLR100W ERG27 3-keto sterol reductase 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs C HDA; HDA; IDA; IDA mitochondrial outer membrane; endoplasmic reticulum; endoplasmic reticulum membrane; lipid particle GO_0005741; GO_0005783; GO_0005789; GO_0005811 4204 YLR101C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W 4205 YLR102C APC9 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition C IDA anaphase-promoting complex GO_0005680 4206 YLR103C CDC45 DNA replication initiation factor DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes C IDA; IDA; IDA; IDA; IDA; IDA nuclear pre-replicative complex; nucleus; replication fork protection complex; CMG complex; nuclear replication fork; DNA replication preinitiation complex GO_0005656; GO_0005634; GO_0031298; GO_0071162; GO_0043596; GO_0031261 4207 YLR104W LCL2 Putative protein of unknown function Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) C HDA fungal-type vacuole GO_0000324 4208 YLR105C SEN2 Subunit of the tRNA splicing endonuclease Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease C IDA; IDA; HDA mitochondrial outer membrane; tRNA-intron endonuclease complex; mitochondrion GO_0005741; GO_0000214; GO_0005739 4209 YLR106C MDN1 Huge dynein-related AAA-type ATPase (midasin) Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus C HDA; IDA; HDA; IDA mitochondrion; nucleoplasm; preribosome, large subunit precursor; nucleus GO_0005739; GO_0005654; GO_0030687; GO_0005634 4210 YLR107W REX3 RNA exonuclease RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4211 YLR108C YLR108C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication C HDA nucleus GO_0005634 4212 YLR109W AHP1 Thiol-specific peroxiredoxin Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p C IDA; HDA; HDA cytoplasm; cytosol; plasma membrane GO_0005737; GO_0005829; GO_0005886 4213 YLR110C CCW12 Cell wall mannoprotein Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication C HDA; IDA; HDA; IDA; IDA cell periphery; cellular bud tip; fungal-type vacuole; mating projection tip; fungal-type cell wall GO_0071944; GO_0005934; GO_0000324; GO_0043332; GO_0009277 4214 YLR111W YLR111W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4215 YLR112W YLR112W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4216 YLR113W HOG1 Mitogen-activated protein kinase involved in osmoregulation Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII in osmotic shock; activates CDC28 by stimulating antisense RNA transcription; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; with Mrc1p defines novel S-phase checkpoint that prevent conflicts between DNA replication and transcription; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4217 YLR114C AVL9 Conserved protein involved in exocytic transport from the Golgi Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA; HDA cytoplasm; cellular bud tip; cellular bud neck GO_0005737; GO_0005934; GO_0005935 4218 YLR115W CFT2 Subunit of the mRNA cleavage and polyadenlylation factor (CPF) Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. C IPI; IDA mRNA cleavage factor complex; mRNA cleavage and polyadenylation specificity factor complex GO_0005849; GO_0005847 4219 YLR116W MSL5 Component of commitment complex Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA commitment complex; cytosol; nucleus GO_0000243; GO_0005829; GO_0005634 4220 YLR117C CLF1 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins C IDA; IDA; IDA; IDA; IDA; IDA U2-type post-mRNA release spliceosomal complex; U2-type catalytic step 1 spliceosome; chromatin; Prp19 complex; U2-type catalytic step 2 spliceosome; U2-type prespliceosome GO_0071008; GO_0071006; GO_0000785; GO_0000974; GO_0071007; GO_0071004 4221 YLR118C TML25 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4222 YLR119W SRN2 Component of the ESCRT-I complex Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus C IPI; HDA; IPI ESCRT I complex; nucleus; endosome GO_0000813; GO_0005634; GO_0005768 4223 YLR120C YPS1 Aspartic protease Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; YPS1 has a paralog, MKC7, that arose from the whole genome duplication C IDA anchored component of plasma membrane GO_0046658 4224 YLR120W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR121C 4225 YLR121C YPS3 Aspartic protease Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor C IDA anchored component of plasma membrane GO_0046658 4226 YLR122C YLR122C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C 4227 YLR123C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif 4228 YLR124W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4229 YLR125W YLR125W Putative protein of unknown function Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene 4230 YLR126C YLR126C Putative glutamine amidotransferase Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA; IDA; HDA cytoplasm; nuclear periphery; nucleus GO_0005737; GO_0034399; GO_0005634 4231 YLR127C APC2 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p C IDA anaphase-promoting complex GO_0005680 4232 YLR128W DCN1 Scaffold-type E3 ligase Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation 4233 YLR129W DIP2 Nucleolar protein Nucleolar protein; specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex C IDA; HDA; IDA; IDA Pwp2p-containing subcomplex of 90S preribosome; 90S preribosome; nucleolus; small-subunit processome GO_0034388; GO_0030686; GO_0005730; GO_0032040 4234 YLR130C ZRT2 Low-affinity zinc transporter of the plasma membrane Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor C HDA; IMP endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 4235 YLR131C ACE2 Transcription factor required for septum destruction after cytokinesis Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication C IDA; IDA; HDA cytosol; nucleus; cytoplasm GO_0005829; GO_0005634; GO_0005737 4236 YLR132C USB1 Putative poly(U)-specific 3'-to-5' RNA exonuclease Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants C IDA; IDA; HDA nucleus; mitochondrion; cytoplasm GO_0005634; GO_0005739; GO_0005737 4237 YLR133W CKI1 Choline kinase Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4238 YLR134W PDC5 Minor isoform of pyruvate decarboxylase Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4239 YLR135W SLX4 Endonuclease involved in processing DNA Endonuclease involved in processing DNA; acts during recombination and repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress C IPI; HDA Slx1-Slx4 complex; nucleus GO_0033557; GO_0005634 4240 YLR136C TIS11 mRNA-binding protein expressed during iron starvation mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4241 YLR137W RKM5 Protein lysine methyltransferase Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species 4242 YLR138W NHA1 Na+/H+ antiporter Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH C IDA; IDA; HDA plasma membrane; membrane raft; endoplasmic reticulum GO_0005886; GO_0045121; GO_0005783 4243 YLR139C SLS1 Mitochondrial membrane protein Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery C HDA; IDA; IDA; IDA mitochondrion; mitochondrial nucleoid; mitochondrial inner membrane; integral component of membrane GO_0005739; GO_0042645; GO_0005743; GO_0016021 4244 YLR140W Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I 4245 YLR141W RRN5 Protein involved in transcription of rDNA by RNA polymerase I Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p C IDA; IDA nucleolus; RNA polymerase I upstream activating factor complex GO_0005730; GO_0000500 4246 YLR142W PUT1 Proline oxidase Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source C HDA mitochondrion GO_0005739 4247 YLR143W DPH6 Diphthamide synthetase Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene C HDA cytoplasm GO_0005737 4248 YLR144C ACF2 Intracellular beta-1,3-endoglucanase Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress C IDA intracellular GO_0005622 4249 YLR145W RMP1 Subunit of RNase MRP Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P C IPI; HDA; HDA; HDA ribonuclease MRP complex; cytoplasm; nucleus; nucleolus GO_0000172; GO_0005737; GO_0005634; GO_0005730 4250 YLR146C SPE4 Spermine synthase Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid C HDA cytoplasm GO_0005737 4251 YLR146W-A YLR146W-A Putative protein of unknown function Putative protein of unknown function 4252 YLR147C SMD3 Core Sm protein Sm D3 Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 C IDA; IDA; IDA; IDA; IPI U5 snRNP; U1 snRNP; U2-type prespliceosome; U4/U6 x U5 tri-snRNP complex; commitment complex GO_0005682; GO_0005685; GO_0071004; GO_0046540; GO_0000243 4253 YLR148W PEP3 Component of CORVET membrane tethering complex Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis C IDA; IDA; IPI; IDA fungal-type vacuole membrane; CORVET complex; HOPS complex; extrinsic component of vacuolar membrane GO_0000329; GO_0033263; GO_0030897; GO_0000306 4254 YLR149C YLR149C Protein of unknown function Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress 4255 YLR149C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4256 YLR150W STM1 Protein required for optimal translation under nutrient stress Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery C HDA; IDA cytoplasm; polysome GO_0005737; GO_0005844 4257 YLR151C PCD1 8-oxo-dGTP diphosphatase 8-oxo-dGTP diphosphatase; prevents spontaneous mutagenesis via sanitization of oxidized purine nucleoside triphosphates; can also act as peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member; similar E. coli MutT and human, rat and mouse MTH1 C IDA; HDA peroxisome; cytosol GO_0005777; GO_0005829 4258 YLR152C YLR152C Putative protein of unknown function Putative protein of unknown function; YLR152C is not an essential gene 4259 YLR153C ACS2 Acetyl-coA synthetase isoform Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions C IDA; IDA; IDA cytosol; nucleolus; nucleus GO_0005829; GO_0005730; GO_0005634 4260 YLR154C RNH203 Ribonuclease H2 subunit Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome C IDA; HDA; HDA ribonuclease H2 complex; nucleus; cytoplasm GO_0032299; GO_0005634; GO_0005737 4261 YLR154C-G YLR154C-G Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 4262 YLR154C-H YLR154C-H Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-2; YLR154C-H has a paralog, YLR157C-C, that arose from a segmental duplication 4263 YLR154W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 25S rRNA gene on the opposite strand 4264 YLR154W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 25S rRNA gene on the opposite strand 4265 YLR154W-C TAR1 Protein potentially involved in regulation of respiratory metabolism Protein potentially involved in regulation of respiratory metabolism; located in the mitochondria; interacts genetically with RPO41 and physically with Coq5p; encoded within the 25S rRNA gene on the opposite strand C IMP mitochondrion GO_0005739 4266 YLR154W-E Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 35S rRNA gene on the opposite strand 4267 YLR154W-F Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 35S rRNA gene on the opposite strand 4268 YLR155C ASP3-1 Cell-wall L-asparaginase II involved in asparagine catabolism Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-1 has a paralog, ASP3-3, that arose from a segmental duplication C HDA; IDA; HDA endoplasmic reticulum; cell wall-bounded periplasmic space; nuclear envelope GO_0005783; GO_0030287; GO_0005635 4269 YLR156C-A YLR156C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-3; YLR156C-A has a paralog, YLR159C-A, that arose from a segmental duplication 4270 YLR156W YLR156W Putative protein of unknown function Putative protein of unknown function; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis; YLR156W has a paralog, YLR159W, that arose from a segmental duplication 4271 YLR157C ASP3-2 Cell-wall L-asparaginase II involved in asparagine catabolism Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-2 has a paralog, ASP3-4, that arose from a segmental duplication C IDA cell wall-bounded periplasmic space GO_0030287 4272 YLR157C-A YLR157C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4273 YLR157C-B YLR157C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4274 YLR157C-C YLR157C-C Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-4; YLR157C-C has a paralog, YLR154C-H, that arose from a segmental duplication 4275 YLR157W-D YLR157W-D Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YLR157W-D has a paralog, YLR161W, that arose from a segmental duplication 4276 YLR157W-E YLR157W-E Putative protein of unknown function identified by gene-trapping Putative protein of unknown function identified by gene-trapping; microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element 4277 YLR158C ASP3-3 Cell-wall L-asparaginase II involved in asparagine catabolism Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication C IDA cell wall-bounded periplasmic space GO_0030287 4278 YLR159C-A YLR159C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-5; YLR159C-A has a paralog, YLR156C-A, that arose from a segmental duplication 4279 YLR159W YLR159W Putative protein of unknown function Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR159W has a paralog, YLR156W, that arose from a segmental duplication 4280 YLR160C ASP3-4 Cell-wall L-asparaginase II involved in asparagine catabolism Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-4 has a paralog, ASP3-2, that arose from a segmental duplication C HDA; IDA cell periphery; cell wall-bounded periplasmic space GO_0071944; GO_0030287 4281 YLR161W YLR161W Putative protein of unknown function Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR161W has a paralog, YLR157W-D, that arose from a segmental duplication 4282 YLR162W YLR162W Protein of unknown function Putative protein of unknown function; overexpression confers resistance to the antimicrobial peptide MiAMP1 and causes growth arrest, apoptosis, and increased sensitivity to cobalt chloride 4283 YLR162W-A RRT15 Putative protein of unknown function Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; identified in a screen for mutants with decreased levels of rDNA transcription 4284 YLR163C MAS1 Beta subunit of the mitochondrial processing protease (MPP) Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins C IDA; HDA mitochondrial processing peptidase complex; mitochondrion GO_0017087; GO_0005739 4285 YLR163W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4286 YLR164W SHH4 Putative alternate subunit of succinate dehydrogenase (SDH) Putative alternate subunit of succinate dehydrogenase (SDH); mitochondrial inner membrane protein; genetic interaction with SDH4 suggests that Shh4p can function as a functional SDH subunit; a fraction copurifies with SDH subunit Sdh3p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Shh4p has greater similarity to human SDHD (subunit D of SDH, implicated in paraganglioma) than does its paralog Sdh4p C IDA; IGI; HDA mitochondrial inner membrane; mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone); mitochondrion GO_0005743; GO_0005749; GO_0005739 4287 YLR165C PUS5 Pseudouridine synthase Pseudouridine synthase; catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability C IMP mitochondrion GO_0005739 4288 YLR166C SEC10 Essential 100kDa subunit of the exocyst complex Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion C HDA; HDA; IDA cellular bud neck; mating projection tip; exocyst GO_0005935; GO_0043332; GO_0000145 4289 YLR167W RPS31 Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog C IDA; HDA cytosolic small ribosomal subunit; cytoplasm GO_0022627; GO_0005737 4290 YLR168C UPS2 Mitochondrial intermembrane space protein Mitochondrial intermembrane space protein; involved in phospholipid metabolism; has role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI; UPS2 has a paralog, UPS3, that arose from the whole genome duplication C HDA; IDA; IDA mitochondrion; mitochondrial intermembrane space; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0005758; GO_0031314 4291 YLR169W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4292 YLR170C APS1 Small subunit of the clathrin-associated adaptor complex AP-1 Small subunit of the clathrin-associated adaptor complex AP-1; AP-1 is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex C IPI AP-1 adaptor complex GO_0030121 4293 YLR171W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4294 YLR172C DPH5 Methyltransferase required for synthesis of diphthamide Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 4295 YLR173W YLR173W Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole membrane GO_0000329 4296 YLR174W IDP2 Cytosolic NADP-specific isocitrate dehydrogenase Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication C IDA cytosol GO_0005829 4297 YLR175W CBF5 Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on large and small rRNAs, on snRNA U2, and on some mRNAs; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs C IPI; HDA box H/ACA snoRNP complex; 90S preribosome GO_0031429; GO_0030686 4298 YLR176C RFX1 Major transcriptional repressor of DNA-damage-regulated genes Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4299 YLR177W YLR177W Putative protein of unknown function Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4300 YLR178C TFS1 Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p) Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA fungal-type vacuole membrane; fungal-type vacuole lumen; cytoplasm GO_0000329; GO_0000328; GO_0005737 4301 YLR179C YLR179C Protein of unknown function with similarity to Tfs1p Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus C IDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4302 YLR180W SAM1 S-adenosylmethionine synthetase S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication C HDA; IDA cytoplasm; cytoplasmic stress granule GO_0005737; GO_0010494 4303 YLR181C VTA1 Multivesicular body (MVB) protein Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain C IDA; IDA; HDA endosome membrane; multivesicular body; endosome GO_0010008; GO_0005771; GO_0005768 4304 YLR182W SWI6 Transcription cofactor Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators C IDA; IDA; IDA; IDA SBF transcription complex; nucleus; cytoplasm; MBF transcription complex GO_0033309; GO_0005634; GO_0005737; GO_0030907 4305 YLR183C TOS4 Putative transcription factor, contains Forkhead Associated domain Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase (HDAC) complexes; APCC(Cdh1) substrate; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication C HDA; HDA; HDA; HDA; IDA Rpd3L-Expanded complex; nucleus; cytoplasm; mitochondrion; nuclear chromatin GO_0070210; GO_0005634; GO_0005737; GO_0005739; GO_0000790 4306 YLR184W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4307 YLR185W RPL37A Ribosomal 60S subunit protein L37A Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication C IDA; IDA cytosolic large ribosomal subunit; preribosome, large subunit precursor GO_0022625; GO_0030687 4308 YLR186W EMG1 Methyltransferase for rRNA Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity C IDA; IDA; IDA; IDA; IDA; IDA; IDA nucleus; nuclear microtubule; nucleolus; 90S preribosome; cytoplasm; small-subunit processome; nuclear periphery GO_0005634; GO_0005880; GO_0005730; GO_0030686; GO_0005737; GO_0032040; GO_0034399 4309 YLR187W SKG3 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication C HDA; HDA; HDA; HDA cellular bud neck; cytoplasm; cellular bud; cellular bud tip GO_0005935; GO_0005737; GO_0005933; GO_0005934 4310 YLR188W MDL1 Mitochondrial inner membrane half-type ABC transporter Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 4311 YLR189C ATG26 UDP-glucose:sterol glucosyltransferase UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy C HDA cytoplasm GO_0005737 4312 YLR190W MMR1 Phosphorylated protein of the mitochondrial outer membrane Phosphorylated protein of the mitochondrial outer membrane; localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p C IDA; HDA; IDA; IDA; IDA mitochondrial outer membrane; mitochondrion; cellular bud neck; incipient cellular bud site; cellular bud GO_0005741; GO_0005739; GO_0005935; GO_0000131; GO_0005933 4313 YLR191W PEX13 Peroxisomal importomer complex component Peroxisomal importomer complex component; integral peroxisomal membrane protein required for docking and translocation of peroxisomal matrix proteins; interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p; forms a complex with Pex14p and Pex17p C IDA; IDA; IDA Pex17p-Pex14p docking complex; peroxisomal importomer complex; peroxisomal membrane GO_1990415; GO_1990429; GO_0005778 4314 YLR192C HCR1 eIF3j component of translation initiation factor 3 (eIF3) eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for processing of 20S pre-rRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA C IDA; IPI cytoplasmic stress granule; eukaryotic translation initiation factor 3 complex GO_0010494; GO_0005852 4315 YLR193C UPS1 Phosphatidic acid transfer protein Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI C IDA; HDA; IDA; IDA mitochondrial intermembrane space; mitochondrion; extrinsic component of mitochondrial inner membrane; mitochondrial inner membrane GO_0005758; GO_0005739; GO_0031314; GO_0005743 4316 YLR194C NCW2 Structural constituent of the cell wall Structural constituent of the cell wall; attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress C HDA; HDA; IDA; IDA fungal-type vacuole; cellular bud neck; anchored component of plasma membrane; fungal-type cell wall GO_0000324; GO_0005935; GO_0046658; GO_0009277 4317 YLR195C NMT1 N-myristoyl transferase N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction C IDA cytosol GO_0005829 4318 YLR196W PWP1 Protein with WD-40 repeats involved in rRNA processing Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily C HDA; HDA; HDA nucleolus; cytoplasm; nucleus GO_0005730; GO_0005737; GO_0005634 4319 YLR197W NOP56 Essential evolutionarily-conserved nucleolar protein Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects C IPI; HDA; HDA; HDA; IDA box C/D snoRNP complex; nucleolus; 90S preribosome; nucleus; small-subunit processome GO_0031428; GO_0005730; GO_0030686; GO_0005634; GO_0032040 4320 YLR198C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W 4321 YLR199C PBA1 Protein involved in 20S proteasome assembly Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly C HDA cytoplasm GO_0005737 4322 YLR200W YKE2 Subunit of the heterohexameric Gim/prefoldin protein complex Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation C IPI; IDA prefoldin complex; cytoplasm GO_0016272; GO_0005737 4323 YLR201C COQ9 Protein required for ubiquinone biosynthesis and respiratory growth Protein required for ubiquinone biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; ubiquinone is also known as coenzyme Q C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 4324 YLR202C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron 4325 YLR203C MSS51 Specific translational activator for the mitochondrial COX1 mRNA Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis C HDA; IDA; IDA mitochondrion; vacuolar membrane; extrinsic component of mitochondrial inner membrane GO_0005739; GO_0005774; GO_0031314 4326 YLR204W QRI5 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA C IDA; HDA integral component of mitochondrial inner membrane; mitochondrion GO_0031305; GO_0005739 4327 YLR205C HMX1 ER localized heme oxygenase ER localized heme oxygenase; involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants C IDA; IDA; IDA membrane; nuclear outer membrane; endoplasmic reticulum GO_0016020; GO_0005640; GO_0005783 4328 YLR206W ENT2 Epsin-like protein required for endocytosis and actin patch assembly Epsin-like protein required for endocytosis and actin patch assembly; functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; ENT2 has a paralog, ENT1, that arose from the whole genome duplication C HDA mating projection tip GO_0043332 4329 YLR207W HRD3 ER membrane protein that plays a central role in ERAD ER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events C IDA; HDA; IPI; IDA; IDA Hrd1p ubiquitin ligase ERAD-L complex; endoplasmic reticulum; endoplasmic reticulum membrane; Hrd1p ubiquitin ligase ERAD-M complex; luminal surveillance complex GO_0000839; GO_0005783; GO_0005789; GO_0000838; GO_0034099 4330 YLR208W SEC13 Structural component of 3 complexes Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress C IDA; IDA; IDA; HDA COPII vesicle coat; nuclear pore outer ring; Seh1-associated complex; cell periphery GO_0030127; GO_0031080; GO_0035859; GO_0071944 4331 YLR209C PNP1 Purine nucleoside phosphorylase Purine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway 4332 YLR210W CLB4 B-type cyclin involved in cell cycle progression B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication C IDA; IDA; HDA nucleus; cytoplasm; spindle pole body GO_0005634; GO_0005737; GO_0005816 4333 YLR211C ATG38 Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy C IDA; IDA; IDA; HDA pre-autophagosomal structure; vacuolar membrane; phosphatidylinositol 3-kinase complex, class III, type I; cytoplasm GO_0000407; GO_0005774; GO_0034271; GO_0005737 4334 YLR212C TUB4 Gamma-tubulin Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress C IPI; HDA; IDA; IDA gamma-tubulin small complex, spindle pole body; spindle pole body; inner plaque of spindle pole body; outer plaque of spindle pole body GO_0000928; GO_0005816; GO_0005822; GO_0005824 4335 YLR213C CRR1 Putative glycoside hydrolase of the spore wall envelope Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation C HDA; IDA fungal-type vacuole; ascospore wall GO_0000324; GO_0005619 4336 YLR214W FRE1 Ferric reductase and cupric reductase Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels C IDA plasma membrane GO_0005886 4337 YLR215C CDC123 Assembly factor for the eIF2 translation initiation factor complex Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein C HDA cytoplasm GO_0005737 4338 YLR216C CPR6 Peptidyl-prolyl cis-trans isomerase (cyclophilin) Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 4339 YLR217W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 4340 YLR218C COA4 Twin Cx(9)C protein involved in cytochrome c oxidase organization Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors C HDA; HDA; IDA nucleus; cytoplasm; mitochondrial intermembrane space GO_0005634; GO_0005737; GO_0005758 4341 YLR219W MSC3 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress 4342 YLR220W CCC1 Vacuolar Fe2+/Mn2+ transporter Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress C HDA; IDA; IDA fungal-type vacuole membrane; Golgi apparatus; fungal-type vacuole GO_0000329; GO_0005794; GO_0000324 4343 YLR221C RSA3 Protein with a likely role in ribosomal maturation Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus C HDA; IDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 4344 YLR222C UTP13 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C IDA; IDA; IDA; HDA small-subunit processome; Pwp2p-containing subcomplex of 90S preribosome; nucleolus; 90S preribosome GO_0032040; GO_0034388; GO_0005730; GO_0030686 4345 YLR222C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene IFH1 4346 YLR223C IFH1 Coactivator, regulates transcription of ribosomal protein (RP) genes Coactivator, regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing; regulated by acetylation and phosphorylation at different growth states via TORC1 signaling; IFH1 has a paralog, CRF1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA CURI complex; nucleus; nucleolus; nuclear chromatin GO_0032545; GO_0005634; GO_0005730; GO_0000790 4347 YLR224W UCC1 F-box protein and component of SCF ubiquitin ligase complexes F-box protein and component of SCF ubiquitin ligase complexes; involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene C IPI SCF ubiquitin ligase complex GO_0019005 4348 YLR225C YLR225C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4349 YLR226W BUR2 Cyclin for the Sgv1p (Bur1p) protein kinase Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription C IPI; HDA cyclin-dependent protein kinase holoenzyme complex; nucleus GO_0000307; GO_0005634 4350 YLR227C ADY4 Structural component of the meiotic outer plaque Structural component of the meiotic outer plaque; outer plaque is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane C IDA meiotic spindle pole body GO_0035974 4351 YLR227W-A YLR227W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4352 YLR227W-B YLR227W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4353 YLR228C ECM22 Sterol regulatory element binding protein Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication C IDA; IDA perinuclear region of cytoplasm; membrane GO_0048471; GO_0016020 4354 YLR229C CDC42 Small rho-like GTPase Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins C IDA; IDA; IDA; HDA; IDA; IDA; IDA; IDA; IDA fungal-type vacuole membrane; cellular bud tip; incipient cellular bud site; cell periphery; mating projection tip; nuclear membrane; cellular bud neck; plasma membrane; septin ring GO_0000329; GO_0005934; GO_0000131; GO_0071944; GO_0043332; GO_0031965; GO_0005935; GO_0005886; GO_0005940 4355 YLR230W Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42/YLR229C gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity 4356 YLR231C BNA5 Kynureninase Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4357 YLR232W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 4358 YLR233C EST1 TLC1 RNA-associated factor involved in telomere length regulation TLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication C IPI; IDA; IDA telomerase holoenzyme complex; nucleolus; nucleus GO_0005697; GO_0005730; GO_0005634 4359 YLR234W TOP3 DNA Topoisomerase III DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity stimulated by RPA and Sgs1p-Top2p-Rmi1p; involved in telomere stability and regulation of mitotic recombination C IPI RecQ helicase-Topo III complex GO_0031422 4360 YLR235C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs TOP3/YLR234W and YLR236C 4361 YLR236C YLR236C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YLR235C 4362 YLR237W THI7 Plasma membrane transporter responsible for the uptake of thiamine Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 4363 YLR238W FAR10 Protein involved in recovery from arrest in response to pheromone Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; FAR10 has a paralog, VPS64, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 4364 YLR239C LIP2 Lipoyl ligase Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups C HDA mitochondrion GO_0005739 4365 YLR240W VPS34 Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery C IDA; IDA; IDA; IDA; HDA; IDA; HDA; IDA; IDA endosome; phosphatidylinositol 3-kinase complex, class III, type I; pre-autophagosomal structure; peroxisome; cytosol; nucleus-vacuole junction; fungal-type vacuole membrane; phosphatidylinositol 3-kinase complex, class III, type II; nuclear pore GO_0005768; GO_0034271; GO_0000407; GO_0005777; GO_0005829; GO_0071561; GO_0000329; GO_0034272; GO_0005643 4366 YLR241W CSC1 Calcium permeable gated cation channel Calcium permeable gated cation channel; may be involved in detoxification; similar to Arabidopsis CSC1 C HDA; HDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 4367 YLR242C ARV1 Cortical ER protein Cortical ER protein; implicated in the membrane insertion of tail-anchored C-terminal single transmembrane domain proteins; may function in transport of glycosylphosphatidylinositol intermediates into ER lumen; required for normal intracellular sterol distribution; human ARV1 required for normal cholesterol and bile acid homeostasis; similar to Nup120p C IDA; IDA; IDA endoplasmic reticulum; Golgi apparatus; cortical endoplasmic reticulum GO_0005783; GO_0005794; GO_0032541 4368 YLR243W GPN3 Putative GTPase with a role in biogenesis of RNA pol II and polIII Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; may have a role in sister chromatid cohesion; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p 4369 YLR244C MAP1 Methionine aminopeptidase Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p C IDA; IDA cytoplasmic stress granule; cytosolic ribosome GO_0010494; GO_0022626 4370 YLR245C CDD1 Cytidine deaminase Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4371 YLR246W ERF2 Subunit of a palmitoyltransferase Subunit of a palmitoyltransferase; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; palmitoyltransferase is composed of Erf2p and Shr5p C IDA; IPI integral component of endoplasmic reticulum membrane; endoplasmic reticulum palmitoyltransferase complex GO_0030176; GO_0031211 4372 YLR247C IRC20 E3 ubiquitin ligase and putative helicase E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci C HDA; HDA mitochondrion; nucleus GO_0005739; GO_0005634 4373 YLR248W RCK2 Protein kinase involved in response to oxidative and osmotic stress Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 4374 YLR249W YEF3 Translation elongation factor 3 Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication C IDA; IDA; IDA ribosome; cytoplasmic stress granule; cytosolic ribosome GO_0005840; GO_0010494; GO_0022626 4375 YLR250W SSP120 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C HDA; HDA fungal-type vacuole; cytoplasm GO_0000324; GO_0005737 4376 YLR251W SYM1 Protein required for ethanol metabolism Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 4377 YLR252W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism 4378 YLR253W MCP2 Mitochondrial protein of unknown function involved in lipid homeostas Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1 C IDA; HDA; IDA integral component of mitochondrial membrane; mitochondrion; mitochondrial inner membrane GO_0032592; GO_0005739; GO_0005743 4379 YLR254C NDL1 Homolog of nuclear distribution factor NudE Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends C HDA; IDA; IDA cytoplasm; cytoplasmic microtubule; nucleus GO_0005737; GO_0005881; GO_0005634 4380 YLR255C YLR255C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4381 YLR256W HAP1 Zinc finger transcription factor Zinc finger transcription factor; involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus C IDA; IDA nucleus; mitochondrion GO_0005634; GO_0005739 4382 YLR256W-A YLR256W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4383 YLR257W YLR257W Protein of unknown function Protein of unknown function; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 4384 YLR258W GSY2 Glycogen synthase Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4385 YLR259C HSP60 Tetradecameric mitochondrial chaperonin Tetradecameric mitochondrial chaperonin; required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 4386 YLR260W LCB5 Minor sphingoid long-chain base kinase Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication C HDA; IDA; IDA; HDA cytosol; Golgi apparatus; membrane; nucleus GO_0005829; GO_0005794; GO_0016020; GO_0005634 4387 YLR261C VPS63 Putative protein of unknown function Putative protein of unknown function; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect; decreased levels of protein in enolase deficient mutant 4388 YLR262C YPT6 Rab family GTPase Rab family GTPase; Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; resides temporarily at the Golgi, dissociates into cytosol upon arrival of the Rab GTPaseYpt32p, which also functions in the late Golgi; Golgi-localized form is bound to GTP, while cytosolic form is GDP-bound; homolog of the mammalian Rab6 C IDA; IDA; IDA cis-Golgi network; Golgi apparatus; cytosol GO_0005801; GO_0005794; GO_0005829 4389 YLR262C-A TMA7 Protein of unknown that associates with ribosomes Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4390 YLR263W RED1 Protein component of the synaptonemal complex axial elements Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p; C IDA lateral element GO_0000800 4391 YLR264C-A YLR264C-A Putative protein of unknown function Putative protein of unknown function 4392 YLR264W RPS28B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 4393 YLR265C NEJ1 Protein involved in regulation of nonhomologous end joining Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p C IPI; IDA DNA ligase IV complex; nucleus GO_0032807; GO_0005634 4394 YLR266C PDR8 Transcription factor Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication C IPI nucleus GO_0005634 4395 YLR267W BOP2 Protein of unknown function Protein of unknown function 4396 YLR268W SEC22 R-SNARE protein R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog C IDA; IDA; IDA; IDA; IDA endoplasmic reticulum; ER to Golgi transport vesicle membrane; SNARE complex; Golgi apparatus; integral component of membrane GO_0005783; GO_0012507; GO_0031201; GO_0005794; GO_0016021 4397 YLR269C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4398 YLR270W DCS1 Non-essential hydrolase involved in mRNA decapping Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication C IDA; HDA; HDA; HDA P-body; mitochondrion; nucleus; cytoplasm GO_0000932; GO_0005739; GO_0005634; GO_0005737 4399 YLR271W CMG1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4400 YLR272C YCS4 Subunit of the condensin complex Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation C IDA; IDA nuclear condensin complex; nucleolus GO_0000799; GO_0005730 4401 YLR273C PIG1 Putative targeting subunit for type-1 protein phosphatase Glc7p Putative targeting subunit for type-1 protein phosphatase Glc7p; tethers Glc7p to Gsy2p glycogen synthase; PIG1 has a paralog, GAC1, that arose from the whole genome duplication C IPI protein phosphatase type 1 complex GO_0000164 4402 YLR274W MCM5 Component of the Mcm2-7 hexameric helicase complex Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase C IDA; IDA; IDA; IPI; IDA; IDA; IDA; IDA nuclear pre-replicative complex; replication fork protection complex; nucleus; DNA replication preinitiation complex; MCM complex; nuclear chromosome, telomeric region; cytoplasm; CMG complex GO_0005656; GO_0031298; GO_0005634; GO_0031261; GO_0042555; GO_0000784; GO_0005737; GO_0071162 4403 YLR275W SMD2 Core Sm protein Sm D2 Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 C IDA; IDA; IDA; IDA U1 snRNP; U5 snRNP; U2-type prespliceosome; U4/U6 x U5 tri-snRNP complex GO_0005685; GO_0005682; GO_0071004; GO_0046540 4404 YLR276C DBP9 DEAD-box protein required for 27S rRNA processing DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain C IDA nucleolus GO_0005730 4405 YLR277C YSH1 Endoribonuclease Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication C IPI; IPI mRNA cleavage factor complex; mRNA cleavage and polyadenylation specificity factor complex GO_0005849; GO_0005847 4406 YLR278C YLR278C Zinc-cluster protein Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene C HDA nucleus GO_0005634 4407 YLR279W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4408 YLR280C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4409 YLR281C RSO55 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene C HDA mitochondrion GO_0005739 4410 YLR282C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition 4411 YLR283W YLR283W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene C HDA; HDA endoplasmic reticulum; mitochondrion GO_0005783; GO_0005739 4412 YLR284C ECI1 Peroxisomal delta3,delta2-enoyl-CoA isomerase Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; ECI1 has a paralog, DCI1, that arose from the whole genome duplication C IDA peroxisome GO_0005777 4413 YLR285C-A YLR285C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 4414 YLR285W NNT1 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination C HDA cytoplasm GO_0005737 4415 YLR286C CTS1 Endochitinase Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p C IDA; IDA; HDA; IDA; HDA; HDA fungal-type cell wall; extracellular region; endoplasmic reticulum; cellular bud neck; nuclear envelope; fungal-type vacuole GO_0009277; GO_0005576; GO_0005783; GO_0005935; GO_0005635; GO_0000324 4416 YLR286W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene CTS1 4417 YLR287C YLR287C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene C HDA cytoplasm GO_0005737 4418 YLR287C-A RPS30A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication C IDA cytoplasmic stress granule GO_0010494 4419 YLR288C MEC3 DNA damage and meiotic pachytene checkpoint protein DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 C IDA checkpoint clamp complex GO_0030896 4420 YLR289W GUF1 Mitochondrial matrix GTPase Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor C HDA; IDA; IDA; HDA mitochondrion; mitochondrial matrix; mitochondrial ribosome; nucleus GO_0005739; GO_0005759; GO_0005761; GO_0005634 4421 YLR290C COQ11 Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene C HDA mitochondrion GO_0005739 4422 YLR291C GCD7 Beta subunit of the translation initiation factor eIF2B Beta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression C IMP; IPI; HDA; HDA eukaryotic translation initiation factor 2B complex; guanyl-nucleotide exchange factor complex; mitochondrion; cytosol GO_0005851; GO_0032045; GO_0005739; GO_0005829 4423 YLR292C SEC72 Non-essential subunit of Sec63 complex Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p C HDA; IPI endoplasmic reticulum; Sec62/Sec63 complex GO_0005783; GO_0031207 4424 YLR293C GSP1 Ran GTPase Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication C HDA; IDA cytosol; nucleus GO_0005829; GO_0005634 4425 YLR294C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 4426 YLR295C ATP14 Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress C IMP; HDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion GO_0000276; GO_0005739 4427 YLR296W YLR296W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4428 YLR297W YLR297W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication C HDA; HDA; HDA; HDA fungal-type vacuole; fungal-type vacuole membrane; nucleus; cytoplasm GO_0000324; GO_0000329; GO_0005634; GO_0005737 4429 YLR298C YHC1 Component of the U1 snRNP complex required for pre-mRNA splicing Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site C IPI; IDA; IDA commitment complex; U1 snRNP; U2-type prespliceosome GO_0000243; GO_0005685; GO_0071004 4430 YLR299C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene ECM38 4431 YLR299W ECM38 Gamma-glutamyltranspeptidase Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation C IDA fungal-type vacuole GO_0000324 4432 YLR300W EXG1 Major exo-1,3-beta-glucanase of the cell wall Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication C IDA; IDA; HDA extracellular region; fungal-type cell wall; fungal-type vacuole GO_0005576; GO_0009277; GO_0000324 4433 YLR301W HRI1 Protein of unknown function that interacts with Sec72p and Hrr25p Protein of unknown function that interacts with Sec72p and Hrr25p C IPI; HDA endoplasmic reticulum membrane; cytosol GO_0005789; GO_0005829 4434 YLR302C YLR302C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4435 YLR303W MET17 O-acetyl homoserine-O-acetyl serine sulfhydrylase O-acetyl homoserine-O-acetyl serine sulfhydrylase; required for Methionine and cysteine biosynthesis C HDA; HDA plasma membrane; cytoplasm GO_0005886; GO_0005737 4436 YLR304C ACO1 Aconitase Aconitase; required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy C HDA; IDA; IDA; IDA mitochondrion; cytosol; mitochondrial nucleoid; mitochondrial matrix GO_0005739; GO_0005829; GO_0042645; GO_0005759 4437 YLR305C STT4 Phosphatidylinositol-4-kinase Phosphatidylinositol-4-kinase; functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization C IDA; HDA plasma membrane; mitochondrion GO_0005886; GO_0005739 4438 YLR306W UBC12 Enzyme that mediates the conjugation of Rub1p Enzyme that mediates the conjugation of Rub1p; a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes 4439 YLR307C-A YLR307C-A Putative protein of unknown function Putative protein of unknown function 4440 YLR307W CDA1 Chitin deacetylase Chitin deacetylase; together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall C HDA prospore membrane GO_0005628 4441 YLR308W CDA2 Chitin deacetylase Chitin deacetylase; together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall 4442 YLR309C IMH1 Protein involved in vesicular transport Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi C IDA; IDA Golgi apparatus; cytosol GO_0005794; GO_0005829 4443 YLR310C CDC25 Membrane bound guanine nucleotide exchange factor Membrane bound guanine nucleotide exchange factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; a membrane bound guanine nucleotide exchange factor is also known as a GEF or GDP-release factor C IDA; IDA; IDA; IDA; IDA endoplasmic reticulum membrane; nucleus; plasma membrane; cytoplasm; cytosol GO_0005789; GO_0005634; GO_0005886; GO_0005737; GO_0005829 4444 YLR311C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4445 YLR312C ATG39 Autophagy receptor with a role in degradation of the ER and nucleus Putative protein of unknown function C IDA perinuclear endoplasmic reticulum GO_0097038 4446 YLR312W-A MRPL15 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 4447 YLR313C SPH1 Protein involved in shmoo formation and bipolar bud site selection Protein involved in shmoo formation and bipolar bud site selection; localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; SPH1 has a paralog, SPA2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cellular bud tip; cellular bud neck; mating projection; incipient cellular bud site GO_0005934; GO_0005935; GO_0005937; GO_0000131 4448 YLR314C CDC3 Component of the septin ring that is required for cytokinesis Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells C HDA; IDA; IPI; IDA; IDA; IDA; IDA cellular bud neck; cellular bud neck septin ring; septin complex; mating projection base; ascospore wall; septin filament array; prospore membrane GO_0005935; GO_0000144; GO_0031105; GO_0001400; GO_0005619; GO_0032160; GO_0005628 4449 YLR315W NKP2 Central kinetochore protein and subunit of the Ctf19 complex Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 C HDA; IDA spindle pole body; kinetochore GO_0005816; GO_0000776 4450 YLR316C TAD3 Subunit of tRNA-specific adenosine-34 deaminase Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4451 YLR317W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C 4452 YLR318W EST2 Reverse transcriptase subunit of the telomerase holoenzyme Reverse transcriptase subunit of the telomerase holoenzyme; essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia C IDA; IDA; IDA; IDA telomerase holoenzyme complex; nucleolus; telomerase catalytic core complex; nucleus GO_0005697; GO_0005730; GO_0000333; GO_0005634 4453 YLR319C BUD6 Actin- and formin-interacting protein Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate C IDA; IDA; IDA; HDA; IDA; IDA; IDA cellular bud neck; mating projection tip; incipient cellular bud site; prospore membrane; cellular bud tip; spindle pole body; polarisome GO_0005935; GO_0043332; GO_0000131; GO_0005628; GO_0005934; GO_0005816; GO_0000133 4454 YLR320W MMS22 Subunit of E3 ubiquitin ligase complex involved in replication repair Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation C IDA; IDA nucleus; Cul8-RING ubiquitin ligase complex GO_0005634; GO_0035361 4455 YLR321C SFH1 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog C IDA RSC complex GO_0016586 4456 YLR322W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth 4457 YLR323C CWC24 General splicing factor General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p C IPI; IDA; IDA spliceosomal complex; U2-type spliceosomal complex; nucleus GO_0005681; GO_0005684; GO_0005634 4458 YLR324W PEX30 ER-resident protein involved in peroxisomal biogenesis Peroxisomal integral membrane protein; involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p; PEX30 has a paralog, PEX31, that arose from the whole genome duplication C HDA; HDA; IDA; HDA; IDA cellular bud; endoplasmic reticulum; peroxisome; cell periphery; integral component of peroxisomal membrane GO_0005933; GO_0005783; GO_0005777; GO_0071944; GO_0005779 4459 YLR325C RPL38 Ribosomal 60S subunit protein L38 Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog C IDA cytosolic large ribosomal subunit GO_0022625 4460 YLR326W YLR326W Putative protein of unknown function Putative protein of unknown function; predicted to be palmitoylated C HDA cell periphery GO_0071944 4461 YLR327C TMA10 Protein of unknown function that associates with ribosomes Protein of unknown function that associates with ribosomes; protein abundance increases in response to DNA replication stress; TMA10 has a paralog, STF2, that arose from the whole genome duplication C IDA; HDA; HDA ribosome; cytoplasm; nucleus GO_0005840; GO_0005737; GO_0005634 4462 YLR328W NMA1 Nicotinic acid mononucleotide adenylyltransferase Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4463 YLR329W REC102 Protein involved in early stages of meiotic recombination Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination C IPI; IDA nucleus; condensed nuclear chromosome GO_0005634; GO_0000794 4464 YLR330W CHS5 Component of the exomer complex Component of the exomer complex; exomer also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus C HDA; IDA; IDA; HDA mating projection tip; cytoplasm; exomer complex; cytosol GO_0043332; GO_0005737; GO_0034044; GO_0005829 4465 YLR331C JIP3 Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 4466 YLR332W MID2 O-glycosylated plasma membrane protein O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication C IDA; HDA; HDA integral component of plasma membrane; fungal-type vacuole; mating projection tip GO_0005887; GO_0000324; GO_0043332 4467 YLR333C RPS25B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 4468 YLR334C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here 4469 YLR335W NUP2 Nucleoporin involved in nucleocytoplasmic transport Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization C HDA; IDA; IDA mitochondrion; nuclear pore nuclear basket; nuclear pore cytoplasmic filaments GO_0005739; GO_0044615; GO_0044614 4470 YLR336C SGD1 Essential nuclear protein Essential nuclear protein; required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia C IPI; IDA; IDA FAL1-SGD1 complex; nucleus; nucleolus GO_0097078; GO_0005634; GO_0005730 4471 YLR337C VRP1 Verprolin, proline-rich actin-associated protein Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) C IDA; IDA; IDA; HDA actin cortical patch; incipient cellular bud site; cellular bud neck; mating projection tip GO_0030479; GO_0000131; GO_0005935; GO_0043332 4472 YLR338W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C 4473 YLR339C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 4474 YLR340W RPP0 Conserved ribosomal protein P0 of the ribosomal stalk Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 C HDA; IDA; IDA; IDA 90S preribosome; cytosolic large ribosomal subunit; preribosome, large subunit precursor; cytoplasm GO_0030686; GO_0022625; GO_0030687; GO_0005737 4475 YLR341W SPO77 Meiosis-specific protein of unknown function Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis C IDA intracellular GO_0005622 4476 YLR342W FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication C IDA; HDA; IDA; HDA; IDA; HDA; IDA; IDA plasma membrane; cellular bud; cellular bud neck; cell periphery; cellular bud tip; mitochondrion; 1,3-beta-D-glucan synthase complex; actin cortical patch GO_0005886; GO_0005933; GO_0005935; GO_0071944; GO_0005934; GO_0005739; GO_0000148; GO_0030479 4477 YLR342W-A YLR342W-A Putative protein of unknown function Putative protein of unknown function 4478 YLR343W GAS2 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p C HDA; HDA fungal-type vacuole; cytoplasm GO_0000324; GO_0005737 4479 YLR344W RPL26A Ribosomal 60S subunit protein L26A Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4480 YLR345W YLR345W Similar to 6-phosphofructo-2-kinase enzymes Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene C HDA cytoplasm GO_0005737 4481 YLR346C CIS1 Protein of unknown function found in mitochondria Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 4482 YLR347C KAP95 Karyopherin beta Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p C IDA; IDA; IDA nuclear pore; cytoplasm; nuclear exosome (RNase complex) GO_0005643; GO_0005737; GO_0000176 4483 YLR347W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF KAP95/YLR347C 4484 YLR348C DIC1 Mitochondrial dicarboxylate carrier Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix C HDA; IDA mitochondrion; mitochondrial envelope GO_0005739; GO_0005740 4485 YLR349W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C 4486 YLR350W ORM2 Protein that mediates sphingolipid homeostasis Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication C IDA; IDA endoplasmic reticulum; SPOTS complex GO_0005783; GO_0035339 4487 YLR351C NIT3 Nit protein Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 4488 YLR352W YLR352W Putative protein of unknown function with similarity to F-box proteins Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene C IDA SCF ubiquitin ligase complex GO_0019005 4489 YLR353W BUD8 Protein involved in bud-site selection Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication C IDA; IMP; IDA incipient cellular bud site; plasma membrane; cellular bud tip GO_0000131; GO_0005886; GO_0005934 4490 YLR354C TAL1 Transaldolase, enzyme in the non-oxidative pentose phosphate pathway Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication C IDA nucleus GO_0005634 4491 YLR355C ILV5 Acetohydroxyacid reductoisomerase and mtDNA binding protein Acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 4492 YLR356W ATG33 Mitochondrial mitophagy-specific protein Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 4493 YLR357W RSC2 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication C IDA RSC complex GO_0016586 4494 YLR358C YLR358C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W 4495 YLR359W ADE13 Adenylosuccinate lyase Adenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency 4496 YLR360W VPS38 Part of a Vps34p phosphatidylinositol 3-kinase complex Part of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated C IDA; HDA phosphatidylinositol 3-kinase complex, class III, type II; fungal-type vacuole membrane GO_0034272; GO_0000329 4497 YLR361C DCR2 Protein phosphatase Phosphoesterase; involved in downregulation of the unfolded protein response (UPR), at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START C HDA fungal-type vacuole GO_0000324 4498 YLR361C-A YLR361C-A Putative protein of unknown function Putative protein of unknown function 4499 YLR362W STE11 Signal transducing MEK kinase Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress C IDA cytoplasm GO_0005737 4500 YLR363C NMD4 Protein that may be involved in nonsense-mediated mRNA decay Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4501 YLR363W-A YLR363W-A Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress C HDA nucleus GO_0005634 4502 YLR364C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR366W 4503 YLR364W GRX8 Glutaredoxin that employs a dithiol mechanism of catalysis Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic C HDA cytoplasm GO_0005737 4504 YLR365W YLR365W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene 4505 YLR366W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A 4506 YLR367W RPS22B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 4507 YLR368W MDM30 F-box component of an SCF ubiquitin protein ligase complex F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains C HDA; HDA; IDA mitochondrion; nucleus; SCF ubiquitin ligase complex GO_0005739; GO_0005634; GO_0019005 4508 YLR369W SSQ1 Mitochondrial hsp70-type molecular chaperone Mitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 4509 YLR370C ARC18 Subunit of the ARP2/3 complex Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches C IDA; IDA mitochondrion; Arp2/3 protein complex GO_0005739; GO_0005885 4510 YLR371W ROM2 Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication C IDA; IDA; IDA; HDA; HDA incipient cellular bud site; mating projection tip; cellular bud tip; nucleus; cell periphery GO_0000131; GO_0043332; GO_0005934; GO_0005634; GO_0071944 4511 YLR372W ELO3 Elongase Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis C IDA endoplasmic reticulum GO_0005783 4512 YLR373C VID22 Glycosylated integral membrane protein localized to plasma membrane Glycosylated integral membrane protein localized to plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles; VID22 has a paralog, ENV11, that arose from the whole genome duplication C IDA; HDA integral component of plasma membrane; nucleus GO_0005887; GO_0005634 4513 YLR374C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W 4514 YLR375W STP3 Zinc-finger protein of unknown function Zinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication C HDA nucleus GO_0005634 4515 YLR376C PSY3 Component of Shu complex (aka PCSS complex) Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C C HDA; IDA; IDA; HDA; IMP cytoplasm; nucleus; Shu complex; cytosol; site of double-strand break GO_0005737; GO_0005634; GO_0097196; GO_0005829; GO_0035861 4516 YLR377C FBP1 Fructose-1,6-bisphosphatase Fructose-1,6-bisphosphatase; key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; glucose starvation results in redistribution to the periplasm; interacts with Vid30p C IDA; IDA cytosol; periplasmic space GO_0005829; GO_0042597 4517 YLR378C SEC61 Conserved ER protein translocation channel Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER C HDA; IDA endoplasmic reticulum; Sec61 translocon complex GO_0005783; GO_0005784 4518 YLR379W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C 4519 YLR380W CSR1 Phosphatidylinositol transfer protein Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; HDA; HDA mitochondrion; lipid particle; endosome; prospore membrane; cytosol GO_0005739; GO_0005811; GO_0005768; GO_0005628; GO_0005829 4520 YLR381W CTF3 Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 C IDA condensed nuclear chromosome kinetochore GO_0000778 4521 YLR382C NAM2 Mitochondrial leucyl-tRNA synthetase Mitochondrial leucyl-tRNA synthetase; also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance C HDA mitochondrion GO_0005739 4522 YLR383W SMC6 Component of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18 C IDA; IDA; HDA; IDA nucleus; site of double-strand break; mitochondrion; Smc5-Smc6 complex GO_0005634; GO_0035861; GO_0005739; GO_0030915 4523 YLR384C IKI3 Subunit of Elongator complex Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) C IDA; IDA; IDA nucleus; Elongator holoenzyme complex; cytoplasm GO_0005634; GO_0033588; GO_0005737 4524 YLR385C SWC7 Protein of unknown function Protein of unknown function; component of the Swr1p complex that incorporates Htz1p into chromatin C IDA; HDA Swr1 complex; nucleus GO_0000812; GO_0005634 4525 YLR386W VAC14 Enzyme regulator Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p C IPI; IDA; IDA PAS complex; fungal-type vacuole membrane; extrinsic component of vacuolar membrane GO_0070772; GO_0000329; GO_0000306 4526 YLR387C REH1 Cytoplasmic 60S subunit biogenesis factor Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains C HDA; IDA; IDA cytoplasm; preribosome, large subunit precursor; cytosolic large ribosomal subunit GO_0005737; GO_0030687; GO_0022625 4527 YLR388W RPS29A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 4528 YLR389C STE23 Metalloprotease Metalloprotease; involved in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family; homolog Axl1p is also involved in processing of pro-a-factor C HDA; IDA mitochondrion; extrinsic component of membrane GO_0005739; GO_0019898 4529 YLR390W ECM19 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 4530 YLR390W-A CCW14 Covalently linked cell wall glycoprotein Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall C IDA; HDA; HDA fungal-type cell wall; cell periphery; mitochondrion GO_0009277; GO_0071944; GO_0005739 4531 YLR392C ART10 Protein of unknown function that contains 2 PY motifs Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene C HDA cytoplasm GO_0005737 4532 YLR393W ATP10 Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6 C HDA; IPI; IDA; HDA mitochondrion; mitochondrial inner membrane; integral component of mitochondrial membrane; cytoplasm GO_0005739; GO_0005743; GO_0032592; GO_0005737 4533 YLR394W CST9 SUMO E3 ligase SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate C IDA; IDA condensed nuclear chromosome; nuclear chromosome GO_0000794; GO_0000228 4534 YLR395C COX8 Subunit VIII of cytochrome c oxidase (Complex IV) Subunit VIII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 4535 YLR396C VPS33 ATP-binding protein that is a subunit of the HOPS and CORVET complexes ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains C IPI; IPI; IDA; IDA; IDA HOPS complex; fungal-type vacuole membrane; fungal-type vacuole; cytosol; CORVET complex GO_0030897; GO_0000329; GO_0000324; GO_0005829; GO_0033263 4536 YLR397C AFG2 ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs C IDA; HDA intracellular; preribosome, large subunit precursor GO_0005622; GO_0030687 4537 YLR398C SKI2 Ski complex component and putative RNA helicase Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome C IDA; IDA Ski complex; cytoplasm GO_0055087; GO_0005737 4538 YLR399C BDF1 Protein involved in transcription initiation Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication C IDA; IDA Swr1 complex; nuclear chromatin GO_0000812; GO_0000790 4539 YLR399W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the characterized ORF BDF1/YLR399C 4540 YLR400W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4541 YLR401C DUS3 Dihydrouridine synthase Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress C IDA; HDA; HDA nuclear periphery; cytoplasm; nucleus GO_0034399; GO_0005737; GO_0005634 4542 YLR402W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4543 YLR403W SFP1 Regulates transcription of ribosomal protein and biogenesis genes Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4544 YLR404W SEI1 Seipin involved in lipid droplet (LD) assembly Seipin protein; involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy C IDA; IDA; IDA cortical endoplasmic reticulum; endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0032541; GO_0005783; GO_0030176 4545 YLR405W DUS4 Dihydrouridine synthase Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p 4546 YLR406C RPL31B Ribosomal 60S subunit protein L31B Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4547 YLR406C-A YLR406C-A Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole GO_0000324 4548 YLR407W YLR407W Putative protein of unknown function Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus 4549 YLR408C BLS1 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene C HDA; IDA endosome; BLOC-1 complex GO_0005768; GO_0031083 4550 YLR409C UTP21 Subunit of U3-containing 90S preribosome and SSU processome complexes Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome C IDA; HDA; IDA; HDA; HDA small-subunit processome; nucleus; Pwp2p-containing subcomplex of 90S preribosome; nucleolus; 90S preribosome GO_0032040; GO_0005634; GO_0034388; GO_0005730; GO_0030686 4551 YLR410W VIP1 Inositol hexakisphosphate and inositol heptakisphosphate kinase Inositol hexakisphosphate and inositol heptakisphosphate kinase; inositol heptakisphosphate (IP7) production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1; inositol hexakisphosphate is also known as IP6 C HDA cytoplasm GO_0005737 4552 YLR410W-A YLR410W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4553 YLR410W-B YLR410W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4554 YLR411W CTR3 High-affinity copper transporter of the plasma membrane High-affinity copper transporter of the plasma membrane; acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae C HDA; IDA endoplasmic reticulum; integral component of plasma membrane GO_0005783; GO_0005887 4555 YLR412C-A YLR412C-A Putative protein of unknown function Putative protein of unknown function 4556 YLR412W BER1 Protein involved in microtubule-related processes Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene C HDA cytoplasm GO_0005737 4557 YLR413W INA1 Protein of unknown function Putative protein of unknown function; not an essential gene; YLR413W has a paralog, FAT3, that arose from the whole genome duplication C HDA; HDA; IDA fungal-type vacuole; cell periphery; plasma membrane GO_0000324; GO_0071944; GO_0005886 4558 YLR414C PUN1 Plasma membrane protein with a role in cell wall integrity Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress C HDA; HDA; IDA; IDA; HDA; HDA cell periphery; endoplasmic reticulum; plasma membrane; membrane raft; cellular bud; cytoplasm GO_0071944; GO_0005783; GO_0005886; GO_0045121; GO_0005933; GO_0005737 4559 YLR415C YLR415C Putative protein of unknown function Putative protein of unknown function; YLR415C is not an essential gene 4560 YLR416C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4561 YLR417W VPS36 Component of the ESCRT-II complex Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome; plays a role in the formation of mutant huntingtin (Htt) aggregates in yeast C IDA ESCRT II complex GO_0000814 4562 YLR418C CDC73 Component of the Paf1p complex Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers C IDA; IPI; IDA transcriptionally active chromatin; Cdc73/Paf1 complex; nucleus GO_0035327; GO_0016593; GO_0005634 4563 YLR419W YLR419W Putative helicase with limited sequence similarity to human Rb protein Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 4564 YLR420W URA4 Dihydroorotase Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4565 YLR421C RPN13 Subunit of the 19S regulatory particle of the 26S proteasome lid Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress C IDA; IDA proteasome storage granule; proteasome regulatory particle, lid subcomplex GO_0034515; GO_0008541 4566 YLR422W DCK1 Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1 Protein of unknown function with similarity to human DOCK proteins; interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein; DOCK proteins act as guanine nucleotide exchange factors C IDA; HDA; IDA plasma membrane; cytoplasm; mitochondrion GO_0005886; GO_0005737; GO_0005739 4567 YLR423C ATG17 Scaffold protein responsible for phagophore assembly site organization Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells C IDA; IDA; HDA; IDA; IDA ATG1/ULK1 kinase complex; cytoplasm; nucleus; NA; pre-autophagosomal structure GO_1990316; GO_0005737; GO_0005634; GO_0120095; GO_0000407 4568 YLR424W SPP382 Essential protein that forms a dimer with Ntr2p Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation C HDA; HDA; HDA; IMP; IDA cytoplasm; mitochondrion; nucleus; U2-type post-mRNA release spliceosomal complex; spliceosomal complex GO_0005737; GO_0005739; GO_0005634; GO_0071008; GO_0005681 4569 YLR425W TUS1 Guanine nucleotide exchange factor (GEF) that modulates Rho1p activity Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate C IDA; IDA incipient cellular bud site; cellular bud neck GO_0000131; GO_0005935 4570 YLR426W TDA5 Putative protein of unknown function Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele C HDA; HDA endoplasmic reticulum; mitochondrion GO_0005783; GO_0005739 4571 YLR427W MAG2 Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 C HDA cytoplasm GO_0005737 4572 YLR428C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 4573 YLR429W CRN1 Coronin Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly C IPI; IDA Arp2/3 protein complex; actin cortical patch GO_0005885; GO_0030479 4574 YLR430W SEN1 ATP-dependent 5' to 3' RNA/DNA and DNA helicase Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS C IDA; IDA; IDA; IDA Nrd1 complex; cytoplasm; nucleus; replication fork GO_0035649; GO_0005737; GO_0005634; GO_0005657 4575 YLR431C ATG23 Peripheral membrane protein required for autophagy and CVT Peripheral membrane protein required for autophagy and CVT; required for cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p C IDA; IDA pre-autophagosomal structure; extrinsic component of membrane GO_0000407; GO_0019898 4576 YLR432W IMD3 Inosine monophosphate dehydrogenase Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication C IDA; HDA cytoplasm; plasma membrane GO_0005737; GO_0005886 4577 YLR433C CNA1 Calcineurin A Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication C IPI calcineurin complex GO_0005955 4578 YLR434C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W 4579 YLR435W TSR2 Protein with a potential role in pre-rRNA processing Protein with a potential role in pre-rRNA processing C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4580 YLR436C ECM30 Protein of unknown function Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 4581 YLR437C DIF1 Protein that regulates nuclear localization of Rnr2p and Rnr4p Protein that regulates nuclear localization of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4582 YLR437C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CAR2/YLR438W 4583 YLR438C-A LSM3 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C IDA; IDA; HDA; IPI; IDA; IDA; HDA U6 snRNP; nucleolus; cytoplasm; small nucleolar ribonucleoprotein complex; U4/U6 x U5 tri-snRNP complex; Lsm1-7-Pat1 complex; nucleus GO_0005688; GO_0005730; GO_0005737; GO_0005732; GO_0046540; GO_1990726; GO_0005634 4584 YLR438W CAR2 L-ornithine transaminase (OTAse) L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA cytoplasm; cytosol; nucleus GO_0005737; GO_0005829; GO_0005634 4585 YLR439W MRPL4 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 4586 YLR440C SEC39 Component of the Dsl1p tethering complex Component of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope C IDA; IDA; IDA; IDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane; Dsl1/NZR complex; nuclear envelope; peroxisome GO_0005783; GO_0005789; GO_0070939; GO_0005635; GO_0005777 4587 YLR441C RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication C HDA 90S preribosome GO_0030686 4588 YLR442C SIR3 Silencing protein Silencing protein; interacts with Sir2p, Sir4p, and histone H3 and H4 tails to establish transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus (residues 840-978) assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; SIR3 has a paralog, ORC1, that arose from the whole genome duplication C IDA; IMP; IDA; IDA; IDA; HDA; IDA chromatin silencing complex; nuclear chromosome, telomeric region; nuclear heterochromatin; chromosome, telomeric region; nuclear telomeric heterochromatin; mitochondrion; nucleolus GO_0005677; GO_0000784; GO_0005720; GO_0000781; GO_0005724; GO_0005739; GO_0005730 4589 YLR443W ECM7 Putative integral membrane protein with a role in calcium uptake Putative integral membrane protein with a role in calcium uptake; non-essential protein; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency C HDA fungal-type vacuole GO_0000324 4590 YLR444C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4591 YLR445W GMC2 Protein involved in meiotic crossing over Protein involved in meiotic crossing over; component of the Synaptonemal Complex (SC) along with Ecm11p; required for the efficient loading of the SC transverse filament protein, Zip1p; promotes SUMOylation of Ecm11p; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor C IDA synaptonemal complex GO_0000795 4592 YLR446W YLR446W Putative hexokinase Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene 4593 YLR447C VMA6 Subunit d of the V0 integral membrane domain of V-ATPase Subunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits C HDA fungal-type vacuole membrane GO_0000329 4594 YLR448W RPL6B Ribosomal 60S subunit protein L6B Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4595 YLR449W FPR4 Peptidyl-prolyl cis-trans isomerase (PPIase) Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR4 has a paralog, FPR3, that arose from the whole genome duplication C HDA; IDA; IDA nucleolus; nucleus; chromatin GO_0005730; GO_0005634; GO_0000785 4596 YLR450W HMG2 HMG-CoA reductase HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication C HDA; HDA; IDA; IDA; IPI endoplasmic reticulum; nuclear periphery; nuclear envelope; endoplasmic reticulum membrane; proteasome complex GO_0005783; GO_0034399; GO_0005635; GO_0005789; GO_0000502 4597 YLR451W LEU3 Zinc-knuckle transcription factor, repressor and activator Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation C IDA nucleus GO_0005634 4598 YLR452C SST2 GTPase-activating protein for Gpa1p GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family C IDA plasma membrane GO_0005886 4599 YLR453C RIF2 Protein that binds to the Rap1p C-terminus Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication C IDA; IPI nuclear chromosome, telomeric region; shelterin complex GO_0000784; GO_0070187 4600 YLR454W FMP27 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 4601 YLR455W PDP3 Component of the NuA3b histone acetyltransferase complex Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA cytosol; nucleus; NuA3b histone acetyltransferase complex; NuA3 histone acetyltransferase complex GO_0005829; GO_0005634; GO_1990468; GO_0033100 4602 YLR456W YLR456W Protein of unknown function Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication 4603 YLR457C NBP1 Spindle pole body (SPB) component Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication C IDA; IDA; IDA central plaque of spindle pole body; integral component of nuclear inner membrane; spindle pole body GO_0005823; GO_0005639; GO_0005816 4604 YLR458W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1/YLR457C gene required for mitosis 4605 YLR459W GAB1 GPI transamidase subunit GPI transamidase subunit; involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI C IMP; HDA GPI-anchor transamidase complex; endoplasmic reticulum GO_0042765; GO_0005783 4606 YLR460C YLR460C Member of the quinone oxidoreductase family Member of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine 4607 YLR461W PAU4 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme C HDA fungal-type vacuole GO_0000324 4608 YLR462W YLR462W Putative protein of unknown function with similarity to helicases Putative protein of unknown function with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII 4609 YLR463C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORFs YLR462W and YLR464W 4610 YLR464W YLR464W Putative protein of unknown function Putative protein of unknown function; intron is predicted but not detected experimentally; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C 4611 YLR465C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 100% of YLR465C overlaps the uncharacterized ORF YLR464W and 86% of YLR465C overlaps the verified gene YRF1-4 4612 YLR466C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W 4613 YLR466C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4614 YLR466W YRF1-4 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 4615 YLR467C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W 4616 YLR467W YRF1-5 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 4617 YML001W YPT7 Rab family GTPase Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 C IDA; IDA; IDA; IPI; HDA; HDA fungal-type vacuole membrane; fungal-type vacuole; vacuole-mitochondrion membrane contact site; retromer, cargo-selective complex; mitochondrial outer membrane; mitochondrion GO_0000329; GO_0000324; GO_1990816; GO_0030906; GO_0005741; GO_0005739 4618 YML002W YML002W Putative protein of unknown function Putative protein of unknown function; expression induced by heat and by calcium shortage 4619 YML003W YML003W Putative protein of unknown function Putative protein of unknown function 4620 YML004C GLO1 Monomeric glyoxalase I Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4621 YML005W TRM12 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family C HDA cytoplasm GO_0005737 4622 YML006C GIS4 CAAX box containing protein of unknown function CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 4623 YML007C-A MIN4 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria C HDA mitochondrion GO_0005739 4624 YML007W YAP1 Basic leucine zipper (bZIP) transcription factor Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication C HDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 4625 YML008C ERG6 Delta(24)-sterol C-methyltransferase Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane C HDA; HDA; IDA; IDA mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; lipid particle GO_0005741; GO_0005739; GO_0005783; GO_0005811 4626 YML009C MRPL39 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 4627 YML009C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs SPT5/YML010W and YML009W-B 4628 YML009W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition 4629 YML010W SPT5 Spt4p/5p (DSIF) transcription elongation factor complex subunit Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair C IDA; HDA; IPI; IDA nucleus; mitochondrion; DSIF complex; rDNA heterochromatin GO_0005634; GO_0005739; GO_0032044; GO_0033553 4630 YML011C RAD33 Protein involved in nucleotide excision repair Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus C HDA nucleus GO_0005634 4631 YML012C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 4632 YML012W ERV25 Member of the p24 family involved in ER to Golgi transport Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, C HDA; IDA fungal-type vacuole; ER to Golgi transport vesicle GO_0000324; GO_0030134 4633 YML013W UBX2 Bridging factor involved in ER-associated protein degradation (ERAD) Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis C IDA; IDA; HDA; HDA; IDA; IDA; IDA lipid particle; integral component of plasma membrane; endoplasmic reticulum; mitochondrial outer membrane; Hrd1p ubiquitin ligase ERAD-L complex; integral component of endoplasmic reticulum membrane; Doa10p ubiquitin ligase complex GO_0005811; GO_0005887; GO_0005783; GO_0005741; GO_0000839; GO_0030176; GO_0000837 4634 YML014W TRM9 tRNA methyltransferase tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4635 YML015C TAF11 TFIID subunit (40 kDa) TFIID subunit (40 kDa); involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors C IDA transcription factor TFIID complex GO_0005669 4636 YML016C PPZ1 Serine/threonine protein phosphatase Z, isoform of Ppz2p Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance C IDA; IDA; HDA nucleus; extrinsic component of plasma membrane; cytoplasm GO_0005634; GO_0019897; GO_0005737 4637 YML017W PSP2 Asn rich cytoplasmic protein that contains RGG motifs Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing C IDA; HDA P-body; cytoplasm GO_0000932; GO_0005737 4638 YML018C YML018C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication C HDA fungal-type vacuole membrane GO_0000329 4639 YML019W OST6 Subunit of the oligosaccharyltransferase complex of the ER lumen Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p C IPI; HDA oligosaccharyltransferase complex; endoplasmic reticulum GO_0008250; GO_0005783 4640 YML020W YML020W Putative protein of unknown function Putative protein of unknown function 4641 YML021C UNG1 Uracil-DNA glycosylase Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus C IDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 4642 YML022W APT1 Adenine phosphoribosyltransferase Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4643 YML023C NSE5 Component of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair C IDA; HDA Smc5-Smc6 complex; nucleus GO_0030915; GO_0005634 4644 YML024W RPS17A Ribosomal protein 51 (rp51) of the small (40s) subunit Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication 4645 YML025C YML6 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 4646 YML026C RPS18B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 4647 YML027W YOX1 Homeobox transcriptional repressor Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication C IDA; HDA; HDA nuclear chromatin; nucleus; cytoplasm GO_0000790; GO_0005634; GO_0005737 4648 YML028W TSA1 Thioredoxin peroxidase Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; required for telomere length maintenance; protein abundance increases, forms cytoplasmic foci during DNA replication stress; TSA1 has a paralog, TSA2, that arose from the whole genome duplication C IDA; IDA; IDA cytoplasm; cytosol; polysome GO_0005737; GO_0005829; GO_0005844 4649 YML029W USA1 Scaffold subunit of the Hrd1p ubiquitin ligase Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p C IPI; IMP; HDA endoplasmic reticulum membrane; Hrd1p ubiquitin ligase ERAD-L complex; endoplasmic reticulum GO_0005789; GO_0000839; GO_0005783 4650 YML030W RCF1 Cytochrome c oxidase subunit Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast C HDA; IDA; IDA mitochondrion; mitochondrial respiratory chain supercomplex; integral component of mitochondrial inner membrane GO_0005739; GO_0097249; GO_0031305 4651 YML031C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF NDC1/YML031W 4652 YML031W NDC1 Subunit of the transmembrane ring of the nuclear pore complex (NPC) Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 C IDA; IPI; IDA spindle pole body; nuclear pore transmembrane ring; nuclear pore GO_0005816; GO_0070762; GO_0005643 4653 YML032C RAD52 Protein that stimulates strand exchange Protein that stimulates strand exchange; stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination C IDA; IDA nucleus; nuclear chromosome GO_0005634; GO_0000228 4654 YML034C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SRC1/YML034W 4655 YML034W SRC1 Inner nuclear membrane protein Inner nuclear membrane protein; highly enriched at telomeres and subtelomeric regions; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance; SEC1 has a paralog, HEH2, that arose from the whole genome duplication C IDA; IDA; HDA chromosome, telomeric region; nuclear envelope; nuclear periphery GO_0000781; GO_0005635; GO_0034399 4656 YML035C AMD1 AMP deaminase AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools C HDA cytoplasm GO_0005737 4657 YML036W CGI121 Component of the EKC/KEOPS complex Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p C IDA EKC/KEOPS complex GO_0000408 4658 YML037C YML037C Putative protein of unknown function Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene C HDA clathrin-coated vesicle GO_0030136 4659 YML038C YMD8 Putative nucleotide sugar transporter Putative nucleotide sugar transporter; has similarity to Vrg4p C HDA COPI-coated vesicle GO_0030137 4660 YML039W YML039W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4661 YML040W YML040W Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4662 YML041C VPS71 Nucleosome-binding component of the SWR1 complex Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting C HDA; IDA nucleus; Swr1 complex GO_0005634; GO_0000812 4663 YML042W CAT2 Carnitine acetyl-CoA transferase Carnitine acetyl-CoA transferase; present in both mitochondria and peroxisomes; transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes C HDA; IDA; IDA mitochondrion; peroxisomal importomer complex; peroxisome GO_0005739; GO_1990429; GO_0005777 4664 YML043C RRN11 Component of the core factor (CF) rDNA transcription factor complex Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p C IDA; IDA nucleolus; RNA polymerase I core factor complex GO_0005730; GO_0070860 4665 YML045W YML045W Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4666 YML045W-A YML045W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4667 YML046W PRP39 U1 snRNP protein involved in splicing U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats C IGI; IDA; IDA commitment complex; U2-type prespliceosome; U1 snRNP GO_0000243; GO_0071004; GO_0005685 4668 YML047C PRM6 Potassium transporter that mediates K+ influx Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; member of a fungal-specific gene family; PRM6 has a paralog, KCH1, that arose from the whole genome duplication C IDA; IDA; IDA; HDA cellular bud tip; mating projection tip; integral component of plasma membrane; fungal-type vacuole GO_0005934; GO_0043332; GO_0005887; GO_0000324 4669 YML047W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF PRM6/YML047C 4670 YML048W GSF2 Endoplasmic reticulum (ER) localized integral membrane protein Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression C HDA; HDA; HDA; IDA cytoplasm; mitochondrial outer membrane; endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005737; GO_0005741; GO_0005783; GO_0030176 4671 YML049C RSE1 Protein involved in pre-mRNA splicing Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport C IDA; IDA U2 snRNP; U2-type prespliceosome GO_0005686; GO_0071004 4672 YML050W AIM32 Protein of unknown function Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss 4673 YML051W GAL80 Transcriptional regulator involved in the repression of GAL genes Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 4674 YML052W SUR7 Plasma membrane protein, component of eisosomes Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches) C IDA; HDA; HDA; IDA; IDA; IDA; IDA plasma membrane; mitochondrion; cell periphery; actin cortical patch; cell cortex; membrane raft; eisosome GO_0005886; GO_0005739; GO_0071944; GO_0030479; GO_0005938; GO_0045121; GO_0032126 4675 YML053C YML053C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4676 YML054C CYB2 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase) Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions C IDA; HDA; IDA mitochondrion; nucleus; mitochondrial intermembrane space GO_0005739; GO_0005634; GO_0005758 4677 YML054C-A YML054C-A Putative protein of unknown function Putative protein of unknown function 4678 YML055W SPC2 Subunit of signal peptidase complex Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p C IDA; HDA signal peptidase complex; endoplasmic reticulum GO_0005787; GO_0005783 4679 YML056C IMD4 Inosine monophosphate dehydrogenase Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4680 YML057C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W 4681 YML057W CMP2 Calcineurin A Calcineurin A; one isoform (the other is Cna1p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CMP2 has a paralog, CNA1, that arose from the whole genome duplication C IPI; HDA calcineurin complex; cytoplasm GO_0005955; GO_0005737 4682 YML058W SML1 Ribonucleotide reductase inhibitor Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4683 YML058W-A HUG1 Ribonucleotide reductase inhibitor Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 4684 YML059C NTE1 Serine esterase Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes C HDA endoplasmic reticulum GO_0005783 4685 YML060W OGG1 Nuclear and mitochondrial glycosylase/lyase Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance C IDA; IDA; IDA mitochondrion; CBF3 complex; nucleus GO_0005739; GO_0031518; GO_0005634 4686 YML061C PIF1 DNA helicase, potent G-quadruplex DNA binder/unwinder DNA helicase, potent G-quadruplex DNA binder/unwinder; promotes DNA synthesis during break-induced replication (BIR); important for crossover recombination; translated from different start sites for two forms localized to either mitochondria or nucleus; nuclear form is catalytic inhibitor of telomerase; mitochondrial form involved in mitochondrial DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells C IDA; IDA; HDA; IDA replication fork; mitochondrial membrane; mitochondrion; nuclear replication fork GO_0005657; GO_0031966; GO_0005739; GO_0043596 4687 YML062C MFT1 Subunit of the THO complex Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance C IDA; IMP; IPI chromosome, telomeric region; THO complex part of transcription export complex; nucleoplasmic THO complex GO_0000781; GO_0000445; GO_0000446 4688 YML063W RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication C HDA 90S preribosome GO_0030686 4689 YML064C TEM1 GTP-binding protein of the Ras superfamily GTP-binding protein of the Ras superfamily; involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis C IDA; IDA spindle pole body; Bfa1-Bub2 complex GO_0005816; GO_1990334 4690 YML065W ORC1 Largest subunit of the origin recognition complex Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; ORC1 has a paralog, SIR3, that arose from the whole genome duplication C IMP; IDA; IDA; IDA nuclear origin of replication recognition complex; nuclear pre-replicative complex; DNA replication preinitiation complex; nucleus GO_0005664; GO_0005656; GO_0031261; GO_0005634 4691 YML066C SMA2 Meiosis-specific prospore membrane protein Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation C IDA; HDA; IDA; HDA prospore membrane; fungal-type vacuole membrane; cytoplasm; fungal-type vacuole GO_0005628; GO_0000329; GO_0005737; GO_0000324 4692 YML067C ERV41 Protein localized to COPII-coated vesicles Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein C IDA; IDA; IDA integral component of Golgi membrane; integral component of endoplasmic reticulum membrane; ER to Golgi transport vesicle GO_0030173; GO_0030176; GO_0030134 4693 YML068W ITT1 Protein that modulates the efficiency of translation termination Protein that modulates the efficiency of translation termination; interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins 4694 YML069W POB3 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA replication fork protection complex; nuclear chromatin; FACT complex; alpha DNA polymerase:primase complex GO_0031298; GO_0000790; GO_0035101; GO_0005658 4695 YML070W DAK1 Dihydroxyacetone kinase Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation C HDA cytoplasm GO_0005737 4696 YML071C COG8 Component of the conserved oligomeric Golgi complex Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C HDA; IMP cytosol; Golgi transport complex GO_0005829; GO_0017119 4697 YML072C TCB3 Cortical ER protein involved in ER-plasma membrane tethering Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact C IDA; HDA; IDA; IDA cortical endoplasmic reticulum; mitochondrion; cellular bud; plasma membrane GO_0032541; GO_0005739; GO_0005933; GO_0005886 4698 YML073C RPL6A Ribosomal 60S subunit protein L6A Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4699 YML074C FPR3 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase) Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication C IDA nucleolus GO_0005730 4700 YML075C HMG1 HMG-CoA reductase HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication C IDA; IDA; HDA nuclear envelope; endoplasmic reticulum membrane; nuclear periphery GO_0005635; GO_0005789; GO_0034399 4701 YML076C WAR1 Homodimeric Zn2Cys6 zinc finger transcription factor Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively C IDA; HDA nucleus; mitochondrion GO_0005634; GO_0005739 4702 YML077W BET5 Core component of transport protein particle (TRAPP) complexes I-III Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) C IDA; IDA; IDA TRAPPI protein complex; TRAPPIII protein complex; TRAPPII protein complex GO_1990070; GO_1990072; GO_1990071 4703 YML078W CPR3 Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin) Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria C HDA mitochondrion GO_0005739 4704 YML079W YML079W Non-essential protein of unknown function Non-essential protein of unknown function; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4705 YML080W DUS1 Dihydrouridine synthase Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 C HDA nucleus GO_0005634 4706 YML081C-A ATP18 Subunit of the mitochondrial F1F0 ATP synthase Subunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms C IMP; HDA; IDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion; mitochondrial proton-transporting ATP synthase complex GO_0000276; GO_0005739; GO_0005753 4707 YML081W TDA9 Transcription factor that regulates acetate production Transcription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication C HDA nucleus GO_0005634 4708 YML082W YML082W Putative protein predicted to have carbon-sulfur lyase activity Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; not an essential gene; YML082W has a paralog, STR2, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4709 YML083C YML083C Protein of unknown function Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions 4710 YML084W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4711 YML085C TUB1 Alpha-tubulin Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication C IPI; HDA; IDA; HDA; IDA tubulin complex; nucleus; spindle pole body; cytoplasm; nuclear periphery GO_0045298; GO_0005634; GO_0005816; GO_0005737; GO_0034399 4712 YML086C ALO1 D-Arabinono-1,4-lactone oxidase D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress C HDA; IDA; HDA mitochondrion; integral component of mitochondrial outer membrane; mitochondrial outer membrane GO_0005739; GO_0031307; GO_0005741 4713 YML087C AIM33 Protein of unknown function, highly conserved across species Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication 4714 YML088W UFO1 F-box receptor protein F-box receptor protein; subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation C IDA; IDA; IDA SCF ubiquitin ligase complex; nucleus; cytoplasm GO_0019005; GO_0005634; GO_0005737 4715 YML089C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage 4716 YML090W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source 4717 YML091C RPM2 Protein subunit of mitochondrial RNase P Protein subunit of mitochondrial RNase P; has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus C IDA; HDA; IDA; IDA; IDA; IDA nucleus; mitochondrion; ribonuclease P complex; P-body; mitochondrial matrix; mitochondrial ribonuclease P complex GO_0005634; GO_0005739; GO_0030677; GO_0000932; GO_0005759; GO_0030678 4718 YML092C PRE8 Alpha 2 subunit of the 20S proteasome Alpha 2 subunit of the 20S proteasome C IDA; IDA proteasome core complex, alpha-subunit complex; proteasome storage granule GO_0019773; GO_0034515 4719 YML093W UTP14 Subunit of U3-containing Small Subunit (SSU) processome complex Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit C IDA; IDA nucleolus; small-subunit processome GO_0005730; GO_0032040 4720 YML094C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO 4721 YML094W GIM5 Subunit of the heterohexameric cochaperone prefoldin complex Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation C IPI; IDA prefoldin complex; cytoplasm GO_0016272; GO_0005737 4722 YML095C RAD10 Single-stranded DNA endonuclease (with Rad1p) Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein C IPI; IDA nucleotide-excision repair factor 1 complex; nucleus GO_0000110; GO_0005634 4723 YML096W YML096W Putative protein with similarity to asparagine synthetases Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 C HDA cytoplasm GO_0005737 4724 YML097C VPS9 Guanine nucleotide exchange factor (GEF) and ubiquitin receptor Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a VPS9 domain; binds ubiquitin C HDA cytosol GO_0005829 4725 YML098W TAF13 TFIID subunit (19 kDa) TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors C IDA; HDA transcription factor TFIID complex; nucleus GO_0005669; GO_0005634 4726 YML099C ARG81 Zinc finger transcription factor involved in arginine-responsive genes Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p C IDA nucleus GO_0005634 4727 YML099W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ARG81/YML099C 4728 YML100W TSL1 Large subunit of trehalose 6-phosphate synthase/phosphatase complex Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication C HDA; IPI cytoplasm; alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) GO_0005737; GO_0005946 4729 YML100W-A YML100W-A Putative protein of unknown function Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 4730 YML101C CUE4 Protein of unknown function Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; cytoplasm GO_0005783; GO_0005737 4731 YML101C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4732 YML102W CAC2 Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA cytosol; CAF-1 complex; nucleus; chromosome, centromeric region GO_0005829; GO_0033186; GO_0005634; GO_0000775 4733 YML103C NUP188 Subunit of the inner ring of the nuclear pore complex (NPC) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 C IDA; IDA nuclear pore inner ring; nuclear pore GO_0044611; GO_0005643 4734 YML104C MDM1 PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs Intermediate filament protein; required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) C HDA; HDA; IDA; HDA cytoplasm; fungal-type vacuole; nucleus-vacuole junction; cell periphery GO_0005737; GO_0000324; GO_0071561; GO_0071944 4735 YML105C SEC65 Subunit of the signal recognition particle (SRP) Subunit of the signal recognition particle (SRP); involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 C IDA signal recognition particle, endoplasmic reticulum targeting GO_0005786 4736 YML106W URA5 Major orotate phosphoribosyltransferase (OPRTase) isozyme Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4737 YML107C PML39 Protein required for nuclear retention of unspliced pre-mRNAs Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes C IDA; HDA nuclear pore; ribosome GO_0005643; GO_0005840 4738 YML108W YML108W Protein of unknown function Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4739 YML109W ZDS2 Protein with a role in regulating Swe1p-dependent polarized growth Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication C IDA; IPI; IDA; IDA cellular bud tip; nucleus; cytoplasm; cellular bud neck GO_0005934; GO_0005634; GO_0005737; GO_0005935 4740 YML110C COQ5 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 4741 YML111W BUL2 Component of the Rsp5p E3-ubiquitin ligase complex Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication C HDA; IMP cytoplasm; ubiquitin ligase complex GO_0005737; GO_0000151 4742 YML112W CTK3 Gamma subunit of C-terminal domain kinase I Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA CTDK-1 complex; nucleolus; nucleoplasm GO_0070692; GO_0005730; GO_0005654 4743 YML113W DAT1 DNA binding protein that recognizes oligo(dA).oligo(dT) tracts DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 4744 YML114C TAF8 TFIID subunit (65 kDa) TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation C HDA; IDA nucleus; transcription factor TFIID complex GO_0005634; GO_0005669 4745 YML115C VAN1 Component of the mannan polymerase I Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant C IDA alpha-1,6-mannosyltransferase complex GO_0000136 4746 YML116W ATR1 Multidrug efflux pump of the major facilitator superfamily Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA vacuole; cell periphery; plasma membrane GO_0005773; GO_0071944; GO_0005886 4747 YML116W-A Putative protein of unknown function 4748 YML117W NAB6 Putative RNA-binding protein Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP C HDA; IDA cytoplasm; cytoplasmic stress granule GO_0005737; GO_0010494 4749 YML118W NGL3 3'-5' exonuclease specific for poly-A RNAs 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication 4750 YML119W YML119W Putative protein of unknown function Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate C HDA cytoplasm GO_0005737 4751 YML120C NDI1 NADH:ubiquinone oxidoreductase NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID C IDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 4752 YML121W GTR1 Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex Cytoplasmic GTPase; forms a heterodimer with Gtr2p to stimulate TORC1 in response to amino acids; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB C HDA; IPI; IDA; IDA; IDA; IDA fungal-type vacuole membrane; Gtr1-Gtr2 GTPase complex; cytoplasm; EGO complex; nucleus; late endosome membrane GO_0000329; GO_1990131; GO_0005737; GO_0034448; GO_0005634; GO_0031902 4753 YML122C YML122C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4754 YML123C PHO84 High-affinity inorganic phosphate (Pi) transporter High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity C HDA; IDA; HDA fungal-type vacuole; integral component of plasma membrane; endoplasmic reticulum GO_0000324; GO_0005887; GO_0005783 4755 YML124C TUB3 Alpha-tubulin Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication C IDA; HDA; IGI spindle pole body; spindle; tubulin complex GO_0005816; GO_0005819; GO_0045298 4756 YML125C PGA3 Putative cytochrome b5 reductase, localized to the plasma membrane Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 4757 YML126C ERG13 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis C HDA nucleus GO_0005634 4758 YML127W RSC9 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway C IDA RSC complex GO_0016586 4759 YML128C MSC1 Protein of unknown function Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated C HDA; HDA; HDA endoplasmic reticulum; mitochondrion; plasma membrane GO_0005783; GO_0005739; GO_0005886 4760 YML129C COX14 Mitochondrial cytochrome c oxidase (complex IV) assembly factor Mitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane C IDA; IDA; IDA; IDA; IDA; HDA integral component of membrane; mitochondrial inner membrane; extrinsic component of membrane; mitochondrial envelope; mitochondrial matrix; mitochondrion GO_0016021; GO_0005743; GO_0019898; GO_0005740; GO_0005759; GO_0005739 4761 YML130C ERO1 Thiol oxidase required for oxidative protein folding in the ER Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds C HDA endoplasmic reticulum GO_0005783 4762 YML131W YML131W Protein of unknown function Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 4763 YML132W COS3 Endosomal protein involved in turnover of plasma membrane proteins Protein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA; IDA; IDA fungal-type vacuole; endosome; plasma membrane GO_0000324; GO_0005768; GO_0005886 4764 YML133C YML133C Putative Y' element ATP-dependent helicase Putative Y' element ATP-dependent helicase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron C HDA mitochondrion GO_0005739 4765 YML133W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C 4766 YML133W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C 4767 YMR001C CDC5 Polo-like kinase Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rholp guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate C IDA; IDA; IDA nucleus; cellular bud neck; spindle pole GO_0005634; GO_0005935; GO_0000922 4768 YMR001C-A YMR001C-A Putative protein of unknown function Putative protein of unknown function 4769 YMR002W MIX17 Mitochondrial intermembrane space protein Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA; IDA cytoplasm; nucleus; mitochondrion; mitochondrial intermembrane space GO_0005737; GO_0005634; GO_0005739; GO_0005758 4770 YMR003W AIM34 Protein of unknown function Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 4771 YMR004W MVP1 Protein required for sorting proteins to the vacuole Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA endosome; cytoplasm; nucleus GO_0005768; GO_0005737; GO_0005634 4772 YMR005W TAF4 TFIID subunit (48 kDa) TFIID subunit (48 kDa); involved in RNA polymerase II transcription initiation; potential Cdc28p substrate C IDA transcription factor TFIID complex GO_0005669 4773 YMR006C PLB2 Phospholipase B (lysophospholipase) involved in lipid metabolism Phospholipase B (lysophospholipase) involved in lipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine C IDA; IDA; HDA fungal-type cell wall; extracellular region; cell periphery GO_0009277; GO_0005576; GO_0071944 4774 YMR007W YMR007W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4775 YMR008C PLB1 Phospholipase B (lysophospholipase) involved in lipid metabolism Phospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication C HDA; HDA; IDA; IDA cell periphery; plasma membrane; endoplasmic reticulum; periplasmic space GO_0071944; GO_0005886; GO_0005783; GO_0042597 4776 YMR009W ADI1 Acireductone dioxygenease involved in methionine salvage pathway Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4777 YMR010W ANY1 Protein involved in phospholipid flippase function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 C IDA; HDA; IDA; HDA trans-Golgi network; endoplasmic reticulum; endosome; cytoplasm GO_0005802; GO_0005783; GO_0005768; GO_0005737 4778 YMR011W HXT2 High-affinity glucose transporter of the major facilitator superfamily High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose C IDA; HDA; HDA plasma membrane; fungal-type vacuole; cell periphery GO_0005886; GO_0000324; GO_0071944 4779 YMR012W CLU1 Subunit of the eukaryotic translation initiation factor 3 (eIF3) Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant C HDA; IPI; IDA cytoplasm; eukaryotic translation initiation factor 3 complex; cytoplasmic stress granule GO_0005737; GO_0005852; GO_0010494 4780 YMR013C SEC59 Dolichol kinase Dolichol kinase; catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation C IDA; HDA endoplasmic reticulum membrane; endoplasmic reticulum GO_0005789; GO_0005783 4781 YMR013C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF SEC59/YML013C 4782 YMR013W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 4783 YMR014W BUD22 Protein required for rRNA maturation and ribosomal subunit biogenesis Protein required for rRNA maturation and ribosomal subunit biogenesis; required for 18S rRNA maturation; also required for small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern C IDA; IDA; HDA 90S preribosome; nucleus; nucleolus GO_0030686; GO_0005634; GO_0005730 4784 YMR015C ERG5 C-22 sterol desaturase C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs C HDA endoplasmic reticulum GO_0005783 4785 YMR016C SOK2 Nuclear protein that negatively regulates pseudohyphal differentiation Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 4786 YMR017W SPO20 Meiosis-specific subunit of the t-SNARE complex Meiosis-specific subunit of the t-SNARE complex; required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog C IDA; IMP; IDA; IDA nucleus; prospore membrane; plasma membrane; SNARE complex GO_0005634; GO_0005628; GO_0005886; GO_0031201 4787 YMR018W PEX9 Putative protein of unknown function with similarity to human PEX5Rp Putative protein of unknown function with similarity to human PEX5Rp; transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene; PEX5Rp is also known as peroxin protein 5 related protein C IDA; IDA; IDA peroxisome; cytosol; peroxisomal membrane GO_0005777; GO_0005829; GO_0005778 4788 YMR019W STB4 Putative transcription factor Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; C HDA mitochondrion GO_0005739 4789 YMR020W FMS1 Polyamine oxidase Polyamine oxidase; converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis C HDA cytoplasm GO_0005737 4790 YMR021C MAC1 Copper-sensing transcription factor Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS C IDA nucleus GO_0005634 4791 YMR022W UBC7 Ubiquitin conjugating enzyme Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly C IDA; IDA; HDA; IDA endoplasmic reticulum membrane; Doa10p ubiquitin ligase complex; endoplasmic reticulum; Hrd1p ubiquitin ligase ERAD-L complex GO_0005789; GO_0000837; GO_0005783; GO_0000839 4792 YMR023C MSS1 Mitochondrial protein Mitochondrial protein; forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 C IDA; HDA; HDA mitochondrial inner membrane; mitochondrion; cytosol GO_0005743; GO_0005739; GO_0005829 4793 YMR024W MRPL3 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 4794 YMR025W CSI1 Subunit of the Cop9 signalosome Subunit of the Cop9 signalosome; which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome C IDA COP9 signalosome GO_0008180 4795 YMR026C PEX12 C3HC4-type RING-finger peroxin and E3 ubiquitin ligase C3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder C IDA; IDA peroxisomal importomer complex; integral component of peroxisomal membrane GO_1990429; GO_0005779 4796 YMR027W YMR027W A metal-dependent phosphatase, part of the DUF89 protein family Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4797 YMR028W TAP42 Essential protein involved in the TOR signaling pathway Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits C IDA; IDA extrinsic component of membrane; cytosol GO_0019898; GO_0005829 4798 YMR029C FAR8 Protein involved in recovery from arrest in response to pheromone Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p C HDA; IDA cytosol; endoplasmic reticulum GO_0005829; GO_0005783 4799 YMR030W RSF1 Protein required for respiratory growth Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes C IDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 4800 YMR030W-A YMR030W-A Putative protein of unknown function Putative protein of unknown function 4801 YMR031C EIS1 Component of the eisosome required for proper eisosome assembly Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication C IDA; HDA; IDA; HDA; HDA; HDA eisosome; cell periphery; membrane raft; mitochondrion; cytoplasm; plasma membrane GO_0032126; GO_0071944; GO_0045121; GO_0005739; GO_0005737; GO_0005886 4802 YMR031W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C 4803 YMR032W HOF1 Protein that regulates actin cytoskeleton organization SH3 domain-containing protein required for cytokinesis; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p C IDA; IPI; IDA; IDA cellular bud neck; HICS complex; cellular bud neck contractile ring; cellular bud neck septin ring GO_0005935; GO_0044697; GO_0000142; GO_0000144 4804 YMR033W ARP9 Component of both the SWI/SNF and RSC chromatin remodeling complexes Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation C IDA; IDA; IDA nucleus; RSC complex; SWI/SNF complex GO_0005634; GO_0016586; GO_0016514 4805 YMR034C RCH1 Putative transporter Putative transporter; member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene C HDA cell periphery GO_0071944 4806 YMR035W IMP2 Catalytic subunit of mitochondrial inner membrane peptidase complex Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificity), and Som1p C IPI mitochondrial inner membrane peptidase complex GO_0042720 4807 YMR036C MIH1 Protein tyrosine phosphatase involved in cell cycle control Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4808 YMR037C MSN2 Stress-responsive transcriptional activator Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress C HDA; IDA; IDA cytoplasm; nucleus; cytosol GO_0005737; GO_0005634; GO_0005829 4809 YMR038C CCS1 Copper chaperone for superoxide dismutase Sod1p Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA; IDA cytosol; mitochondrion; nucleus; mitochondrial inner membrane GO_0005829; GO_0005739; GO_0005634; GO_0005743 4810 YMR039C SUB1 Transcriptional regulator Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress C IDA nucleus GO_0005634 4811 YMR040W YET2 Protein of unknown function that may interact with ribosomes Protein of unknown function that may interact with ribosomes; based on co-purification experiments; homolog of human BAP31 protein; YET2 has a paralog, YET1, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; ribosome GO_0005783; GO_0005840 4812 YMR041C ARA2 NAD-dependent arabinose dehydrogenase NAD-dependent arabinose dehydrogenase; involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase 4813 YMR042W ARG80 Transcription factor involved in regulating arginine-responsive genes Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p C HDA nucleus GO_0005634 4814 YMR043W MCM1 Transcription factor Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; nuclear chromatin; nucleus GO_0005829; GO_0000790; GO_0005634 4815 YMR044W IOC4 Member of a complex (Isw1b) with Isw1p and Ioc2p Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing C IDA Isw1b complex GO_0036437 4816 YMR045C YMR045C Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4817 YMR046C YMR046C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4818 YMR046W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4819 YMR047C NUP116 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication C IDA; IDA; IDA nuclear pore central transport channel; nuclear pore; nuclear pore cytoplasmic filaments GO_0044613; GO_0005643; GO_0044614 4820 YMR048W CSM3 Replication fork associated factor Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress C HDA; HDA; IDA nucleus; cytoplasm; replication fork protection complex GO_0005634; GO_0005737; GO_0031298 4821 YMR049C ERB1 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 C HDA; HDA; HDA; IDA nucleus; nucleolus; preribosome, large subunit precursor; PeBoW complex GO_0005634; GO_0005730; GO_0030687; GO_0070545 4822 YMR050C YMR050C Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 4823 YMR051C YMR051C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 4824 YMR052C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4825 YMR052W FAR3 Protein of unknown function Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA endoplasmic reticulum; Golgi cis cisterna; cell periphery GO_0005783; GO_0000137; GO_0071944 4826 YMR053C STB2 Protein that interacts with Sin3p in a two-hybrid assay Protein that interacts with Sin3p in a two-hybrid assay; part of a large protein complex with Sin3p and Stb1p; STB2 has a paralog, STB6, that arose from the whole genome duplication C IPI Sin3-type complex GO_0070822 4827 YMR054W STV1 Subunit a of the vacuolar-ATPase V0 domain Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole C IDA; IDA; IDA vacuolar proton-transporting V-type ATPase, V0 domain; Golgi apparatus; late endosome GO_0000220; GO_0005794; GO_0005770 4828 YMR055C BUB2 Mitotic exit network regulator Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage C IDA; IDA Bfa1-Bub2 complex; spindle pole body GO_1990334; GO_0005816 4829 YMR056C AAC1 Mitochondrial inner membrane ADP/ATP translocator Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 4830 YMR057C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 4831 YMR058W FET3 Ferro-O2-oxidoreductase Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA; HDA high-affinity iron permease complex; endoplasmic reticulum; plasma membrane; fungal-type vacuole GO_0033573; GO_0005783; GO_0005886; GO_0000324 4832 YMR059W SEN15 Subunit of the tRNA splicing endonuclease Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p C IDA tRNA-intron endonuclease complex GO_0000214 4833 YMR060C SAM37 Component of the Sorting and Assembly Machinery (SAM) complex Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability C HDA; HDA; IDA mitochondrion; mitochondrial outer membrane; mitochondrial sorting and assembly machinery complex GO_0005739; GO_0005741; GO_0001401 4834 YMR061W RNA14 Component of the cleavage and polyadenylation factor I (CF I) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IPI nucleus; mitochondrion; cytosol; mRNA cleavage factor complex GO_0005634; GO_0005739; GO_0005829; GO_0005849 4835 YMR062C ARG7 Mitochondrial ornithine acetyltransferase Mitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 4836 YMR063W RIM9 Plasma membrane protein of unknown function Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; C IDA plasma membrane GO_0005886 4837 YMR064W AEP1 Protein required for expression of the mitochondrial OLI1 gene Protein required for expression of the mitochondrial OLI1 gene; mitochondrial OLI1 gene encodes subunit 9 of F1-F0 ATP synthase C HDA mitochondrion GO_0005739 4838 YMR065W KAR5 Protein required for nuclear membrane fusion during karyogamy Protein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone C IDA; HDA endoplasmic reticulum membrane; mitochondrion GO_0005789; GO_0005739 4839 YMR066W SOV1 Mitochondrial protein of unknown function Mitochondrial protein of unknown function C HDA mitochondrion GO_0005739 4840 YMR067C UBX4 UBX domain-containing protein that interacts with Cdc48p UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress C HDA; IDA; HDA nucleus; extrinsic component of membrane; cytoplasm GO_0005634; GO_0019898; GO_0005737 4841 YMR068W AVO2 Component of a complex containing the Tor2p kinase and other proteins Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth C HDA; HDA; HDA; IPI cytoplasm; nucleus; cytosol; TORC2 complex GO_0005737; GO_0005634; GO_0005829; GO_0031932 4842 YMR069W NAT4 N alpha-acetyl-transferase N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4843 YMR070W MOT3 Transcriptional repressor, activator Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth and ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; relocalizes to the cytosol in response to hypoxia; can form [MOT3+] prion C HDA; IDA nucleus; cytosol GO_0005634; GO_0005829 4844 YMR071C TVP18 Integral membrane protein Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments C IDA; HDA integral component of Golgi membrane; clathrin-coated vesicle GO_0030173; GO_0030136 4845 YMR072W ABF2 Mitochondrial DNA-binding protein Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication C HDA; IDA; IDA mitochondrion; mitochondrial chromosome; mitochondrial nucleoid GO_0005739; GO_0000262; GO_0042645 4846 YMR073C IRC21 Protein of unknown function Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study C HDA cytoplasm GO_0005737 4847 YMR074C SDD2 Protein with homology to human PDCD5 Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4848 YMR075C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W 4849 YMR075W RCO1 Essential component of the Rpd3S histone deacetylase complex Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p C IDA; IDA; HDA Rpd3S complex; histone deacetylase complex; nucleus GO_0032221; GO_0000118; GO_0005634 4850 YMR076C PDS5 Cohesion maintenance factor Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; also required during meiosis; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA nuclear cohesin complex; condensed nuclear chromosome; cytosol; nucleus; nuclear mitotic cohesin complex GO_0000798; GO_0000794; GO_0005829; GO_0005634; GO_0034990 4851 YMR077C VPS20 Myristoylated subunit of the ESCRT-III complex Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes C IDA; IDA; HDA ESCRT III complex; cytoplasm; cytosol GO_0000815; GO_0005737; GO_0005829 4852 YMR078C CTF18 Subunit of a complex with Ctf8p Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint C IPI; HDA; IDA Ctf18 RFC-like complex; mitochondrion; nuclear replication fork GO_0031390; GO_0005739; GO_0043596 4853 YMR079W SEC14 Phosphatidylinositol/phosphatidylcholine transfer protein Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cytosol; Golgi apparatus; cytoplasm; Golgi membrane GO_0005829; GO_0005794; GO_0005737; GO_0000139 4854 YMR080C NAM7 ATP-dependent RNA helicase of the SFI superfamily ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; HDA mitochondrion; polysome; cytoplasm GO_0005739; GO_0005844; GO_0005737 4855 YMR081C ISF1 Serine-rich, hydrophilic protein Serine-rich, hydrophilic protein; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; ISF1 has a paralog, MBR1, that arose from the whole genome duplication 4856 YMR082C YMR082C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4857 YMR083W ADH3 Mitochondrial alcohol dehydrogenase isozyme III Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 4858 YMR084W YMR084W Putative protein of unknown function Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 4859 YMR085W YMR085W Putative protein of unknown function Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 4860 YMR086C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4861 YMR086W SEG1 Component of eisosome required for proper eisosome assembly Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication C HDA; IDA; HDA; HDA cytoplasm; eisosome; cell periphery; ribosome GO_0005737; GO_0032126; GO_0071944; GO_0005840 4862 YMR087W YMR087W Putative ADP-ribose-1''-monophosphatase Putative ADP-ribose-1''-monophosphatase; converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain 4863 YMR088C VBA1 Permease of basic amino acids in the vacuolar membrane Permease of basic amino acids in the vacuolar membrane C IDA fungal-type vacuole membrane GO_0000329 4864 YMR089C YTA12 Mitochondrial inner membrane m-AAA protease component Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes C IDA; HDA; HDA; IDA; IDA mitochondrial inner boundary membrane; cytoplasm; mitochondrion; mitochondrial inner membrane; m-AAA complex GO_0097002; GO_0005737; GO_0005739; GO_0005743; GO_0005745 4865 YMR090W YMR090W Putative protein of unknown function Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability C HDA cytoplasm GO_0005737 4866 YMR091C NPL6 Component of the RSC chromatin remodeling complex Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions; involved in nuclear protein import and maintenance of proper telomere length C IDA; HDA RSC complex; nucleus GO_0016586; GO_0005634 4867 YMR092C AIP1 Actin cortical patch component Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress C HDA; HDA; IDA; HDA; IDA mating projection tip; cytoplasm; actin cortical patch; nucleus; actin filament GO_0043332; GO_0005737; GO_0030479; GO_0005634; GO_0005884 4868 YMR093W UTP15 Nucleolar protein Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA C IDA; HDA; IDA; IDA; IDA t-UTP complex; 90S preribosome; rDNA heterochromatin; nucleolus; small-subunit processome GO_0034455; GO_0030686; GO_0033553; GO_0005730; GO_0032040 4869 YMR094W CTF13 Subunit of the CBF3 complex Subunit of the CBF3 complex; CBF3 binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis C IDA CBF3 complex GO_0031518 4870 YMR095C SNO1 Protein of unconfirmed function Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase C IPI; HDA glutaminase complex; cytoplasm GO_1903600; GO_0005737 4871 YMR096W SNZ1 Protein involved in vitamin B6 biosynthesis Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins C IPI glutaminase complex GO_1903600 4872 YMR097C MTG1 Putative GTPase peripheral to the mitochondrial inner membrane Putative GTPase peripheral to the mitochondrial inner membrane; essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals C HDA; HDA; IDA mitochondrion; nucleus; mitochondrial inner membrane GO_0005739; GO_0005634; GO_0005743 4873 YMR098C ATP25 Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA; also required for the Oli1p ring formation; YMR098C is not an essential gene C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 4874 YMR099C YMR099C Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase) Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase); likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 4875 YMR100W MUB1 MYND domain-containing protein MYND domain-containing protein; required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene C IPI MUB1-RAD6-UBR2 ubiquitin ligase complex GO_1990304 4876 YMR101C SRT1 Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1 Cis-prenyltransferase; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase C IDA; HDA dehydrodolichyl diphosphate synthase complex; lipid particle GO_1904423; GO_0005811 4877 YMR102C YMR102C Protein of unknown function Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; not an essential gene; YMR102C has a paralog, DGR2, that arose from the whole genome duplication 4878 YMR103C YMR103C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4879 YMR104C YPK2 Protein kinase similar to S/T protein kinase Ypk1p Protein kinase similar to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4880 YMR105C PGM2 Phosphoglucomutase Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 4881 YMR105W-A YMR105W-A Putative protein of unknown function Putative protein of unknown function 4882 YMR106C YKU80 Subunit of telomeric Ku complex (Yku70p-Yku80p) Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair C IMP; IDA nuclear chromosome, telomeric region; Ku70:Ku80 complex GO_0000784; GO_0043564 4883 YMR107W SPG4 Protein required for high temperature survival during stationary phase Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources 4884 YMR108W ILV2 Acetolactate synthase Acetolactate synthase; catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control C HDA; IDA mitochondrion; acetolactate synthase complex GO_0005739; GO_0005948 4885 YMR109W MYO5 One of two type I myosin motors One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication C HDA; IDA; HDA; HDA mating projection tip; actin cortical patch; cell periphery; cellular bud GO_0043332; GO_0030479; GO_0071944; GO_0005933 4886 YMR110C HFD1 Dehydrogenase involved in ubiquinone and sphingolipid metabolism Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder C HDA; HDA; HDA; HDA; IDA; HDA endosome; lipid particle; mitochondrial outer membrane; mitochondrion; integral component of mitochondrial outer membrane; endoplasmic reticulum GO_0005768; GO_0005811; GO_0005741; GO_0005739; GO_0031307; GO_0005783 4887 YMR111C YMR111C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4888 YMR112C MED11 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein C IDA core mediator complex GO_0070847 4889 YMR113W FOL3 Dihydrofolate synthetase, involved in folic acid biosynthesis Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4890 YMR114C YMR114C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene C HDA; HDA; HDA cytoplasm; nucleus; ribosome GO_0005737; GO_0005634; GO_0005840 4891 YMR115W MGR3 Subunit of the mitochondrial (mt) i-AAA protease supercomplex Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; i-AAA complex GO_0005739; GO_0005743; GO_0031942 4892 YMR116C ASC1 G-protein beta subunit and guanine dissociation inhibitor for Gpa2p G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation C IDA; HDA cytosolic small ribosomal subunit; cytoplasm GO_0022627; GO_0005737 4893 YMR117C SPC24 Component of the kinetochore-associated Ndc80 complex Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p C IPI; IPI; IDA condensed nuclear chromosome kinetochore; condensed nuclear chromosome, centromeric region; Ndc80 complex GO_0000778; GO_0000780; GO_0031262 4894 YMR118C SHH3 Putative mitochondrial inner membrane protein of unknown function Putative mitochondrial inner membrane protein of unknown function; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 4895 YMR119W ASI1 Subunit of the inner nuclear membrane Asi ubiquitin ligase complex Subunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI1 has a paralog, ASI3, that arose from the whole genome duplication C IDA; IDA; HDA nuclear inner membrane; Asi complex; nuclear periphery GO_0005637; GO_0097658; GO_0034399 4896 YMR119W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of ORF ASI1/YMR119W 4897 YMR120C ADE17 Enzyme of 'de novo' purine biosynthesis Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine C HDA; IDA plasma membrane; cytosol GO_0005886; GO_0005829 4898 YMR121C RPL15B Ribosomal 60S subunit protein L15B Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress C HDA; HDA; HDA; IDA nucleus; cytoplasm; nucleolus; cytosolic large ribosomal subunit GO_0005634; GO_0005737; GO_0005730; GO_0022625 4899 YMR122C YMR122C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4900 YMR122W-A YMR122W-A Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and endoplasmic reticulum C HDA; HDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 4901 YMR123W PKR1 V-ATPase assembly factor V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress C IDA; HDA integral component of endoplasmic reticulum membrane; endoplasmic reticulum GO_0030176; GO_0005783 4902 YMR124W EPO1 Protein involved in septin-ER tethering Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication C IPI; HDA cellular bud neck; cytoplasm GO_0005935; GO_0005737 4903 YMR125W STO1 Large subunit of the nuclear mRNA cap-binding protein complex Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 C IDA; IPI; HDA; IDA nuclear cap binding complex; commitment complex; nucleus; polysome GO_0005846; GO_0000243; GO_0005634; GO_0005844 4904 YMR126C DLT1 Protein of unknown function Protein of unknown function; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) 4905 YMR127C SAS2 Histone acetyltransferase (HAT) catalytic subunit of the SAS complex Histone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p C IDA; IDA nuclear chromatin; SAS acetyltransferase complex GO_0000790; GO_0033255 4906 YMR128W ECM16 Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; predominantly nucleolar in distribution; required for 18S rRNA synthesis C IDA; HDA; IDA; HDA small-subunit processome; 90S preribosome; nucleolus; mitochondrion GO_0032040; GO_0030686; GO_0005730; GO_0005739 4907 YMR129W POM152 Glycoprotein subunit of transmembrane ring of nuclear pore complex Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; homologous to human NUP210 C IPI; IDA; IDA; HDA; HDA; HDA nuclear pore transmembrane ring; nuclear pore; nuclear envelope lumen; cell periphery; mitochondrion; nuclear periphery GO_0070762; GO_0005643; GO_0005641; GO_0071944; GO_0005739; GO_0034399 4908 YMR130W DPI35 Putative protein of unknown function Putative protein of unknown function; YMR130W is not an essential gene 4909 YMR131C RRB1 Specific chaperone for ribosomal protein Rpl3p Nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p; essential gene C IDA nucleolus GO_0005730 4910 YMR132C JLP2 Protein of unknown function Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) C HDA cytoplasm GO_0005737 4911 YMR133W REC114 Protein involved in early stages of meiotic recombination Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division C IDA condensed nuclear chromosome GO_0000794 4912 YMR134W ERG29 Protein of unknown function involved in ergosterol biosynthesis Protein of unknown function involved in ergosterol biosynthesis; conditional mutants produce less ergosterol, display impaired oxygen consumption, respiratory growth, mitochondrial iron utilization, and are more sensitive to oxidative stress; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; protein localizes to the cytoplasm, ER and nuclear envelope; highly conserved in ascomycetes C HDA; HDA; HDA nuclear envelope; endoplasmic reticulum; cytoplasm GO_0005635; GO_0005783; GO_0005737 4913 YMR135C GID8 Subunit of GID Complex, binds strongly to central component Vid30p Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START C IDA; HDA; HDA GID complex; nucleus; cytoplasm GO_0034657; GO_0005634; GO_0005737 4914 YMR135W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4915 YMR136W GAT2 Protein containing GATA family zinc finger motifs Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine 4916 YMR137C PSO2 Nuclease required for DNA single- and double-strand break repair Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress C HDA nucleus GO_0005634 4917 YMR138W CIN4 GTP-binding protein involved in beta-tubulin (Tub2p) folding GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog C HDA cytoplasm GO_0005737 4918 YMR139W RIM11 Protein kinase Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 4919 YMR140W SIP5 Protein of unknown function Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress C HDA cytoplasm GO_0005737 4920 YMR141C YMR141C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4921 YMR141W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene RPL13B/YMR142C 4922 YMR142C RPL13B Ribosomal 60S subunit protein L13B Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4923 YMR143W RPS16A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication C HDA 90S preribosome GO_0030686 4924 YMR144W FDO1 Protein involved in directionality of mating type switching Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene C HDA nucleus GO_0005634 4925 YMR145C NDE1 Mitochondrial external NADH dehydrogenase Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 4926 YMR146C TIF34 eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3) eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; stimulates rate of ribosomal scanning during translation reinitiation C IDA; IDA; IDA cytoplasmic stress granule; eukaryotic translation initiation factor 3 complex; multi-eIF complex GO_0010494; GO_0005852; GO_0043614 4927 YMR147W LDO45 Putative protein of unknown function Putative protein of unknown function C HDA cell periphery GO_0071944 4928 YMR148W OSW5 Protein of unknown function with possible role in spore wall assembly Protein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion 4929 YMR149W SWP1 Delta subunit of the oligosaccharyl transferase glycoprotein complex Delta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum C IPI oligosaccharyltransferase complex GO_0008250 4930 YMR150C IMP1 Catalytic subunit of mitochondrial inner membrane peptidase complex Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificty) and Som1p C IPI; HDA mitochondrial inner membrane peptidase complex; mitochondrion GO_0042720; GO_0005739 4931 YMR151W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 4932 YMR152W YIM1 Protein of unknown function Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA; IDA endoplasmic reticulum; mitochondrion; cytoplasm; lipid particle GO_0005783; GO_0005739; GO_0005737; GO_0005811 4933 YMR153C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of verified gene NUP53/YMR153W 4934 YMR153W NUP53 FG-nucleoporin component of central core of nuclear pore complex (NPC) FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication C IDA; IDA; IDA nuclear pore; nuclear pore nuclear basket; nuclear pore central transport channel GO_0005643; GO_0044615; GO_0044613 4935 YMR154C RIM13 Calpain-like cysteine protease Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB 4936 YMR155W YMR155W Putative protein of unknown function Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen C HDA fungal-type vacuole GO_0000324 4937 YMR156C TPP1 DNA 3'-phosphatase DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase 4938 YMR157C AIM36 Protein of unknown function Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 4939 YMR158C-A YMR158C-A Putative protein of unknown function Putative protein of unknown function; may contain a lipid attachment site; YMR158C-A is not an essential gene 4940 YMR158W MRPS8 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 4941 YMR158W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C 4942 YMR159C ATG16 Conserved protein involved in autophagy Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA; HDA; IDA; IDA; IDA; IDA; HDA cytoplasm; nucleus; pre-autophagosomal structure; NA; Atg12-Atg5-Atg16 complex; NA; cytosol GO_0005737; GO_0005634; GO_0000407; GO_0120095; GO_0034274; GO_0061908; GO_0005829 4943 YMR160W YMR160W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress C HDA fungal-type vacuole membrane GO_0000329 4944 YMR161W HLJ1 Co-chaperone for Hsp40p Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ C HDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 4945 YMR162C DNF3 Trans-golgi network aminophospholipid translocase (flippase) Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase C IDA; IDA; IDA; HDA trans-Golgi network; mating projection tip membrane; trans-Golgi network transport vesicle; endoplasmic reticulum GO_0005802; GO_0070867; GO_0030140; GO_0005783 4946 YMR163C INP2 Peroxisome-specific receptor important for peroxisome inheritance Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene C HDA; IDA; IDA cytoplasm; peroxisome; integral component of peroxisomal membrane GO_0005737; GO_0005777; GO_0005779 4947 YMR164C MSS11 Transcription factor Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1 4948 YMR165C PAH1 Mg2+-dependent phosphatidate (PA) phosphatase Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 C IDA; IDA; IDA; IDA; HDA; IDA extrinsic component of membrane; cytosol; nuclear membrane; vacuole; cytoplasm; lipid particle GO_0019898; GO_0005829; GO_0031965; GO_0005773; GO_0005737; GO_0005811 4949 YMR166C MME1 Transporter of the mitochondrial inner membrane that exports magnesium Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene C IDA mitochondrial inner membrane GO_0005743 4950 YMR167W MLH1 Protein required for mismatch repair in mitosis and meiosis Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer C HDA; IPI; HDA; IPI; IPI; HDA mitochondrion; MutLalpha complex; nucleus; MutLgamma complex; MutLbeta complex; cytoplasm GO_0005739; GO_0032389; GO_0005634; GO_0097587; GO_0032390; GO_0005737 4951 YMR168C CEP3 Essential kinetochore protein Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain C IDA; IDA CBF3 complex; condensed nuclear chromosome kinetochore GO_0031518; GO_0000778 4952 YMR169C ALD3 Cytoplasmic aldehyde dehydrogenase Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose 4953 YMR170C ALD2 Cytoplasmic aldehyde dehydrogenase Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p C HDA cytoplasm GO_0005737 4954 YMR171C EAR1 Specificity factor required for Rsp5p-dependent ubiquitination Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p C HDA; HDA endosome; endoplasmic reticulum GO_0005768; GO_0005783 4955 YMR172C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF HOT1/YMR172W 4956 YMR172W HOT1 Transcription factor for glycerol biosynthetic genes Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p C IDA nucleus GO_0005634 4957 YMR173W DDR48 DNA damage-responsive protein DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 4958 YMR173W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W 4959 YMR174C PAI3 Cytoplasmic proteinase A (Pep4p) inhibitor Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4960 YMR175W SIP18 Phospholipid-binding hydrophilin Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication C IDA cytoplasm GO_0005737 4961 YMR175W-A YMR175W-A Putative protein of unknown function Putative protein of unknown function 4962 YMR176W ECM5 Subunit of the Snt2C complex Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA; HDA Snt2C complex; nucleus; cytoplasm GO_0070211; GO_0005634; GO_0005737 4963 YMR177W MMT1 Putative metal transporter involved in mitochondrial iron accumulation Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 4964 YMR178W YMR178W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 4965 YMR179W SPT21 Protein with a role in transcriptional silencing Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress C HDA nucleus GO_0005634 4966 YMR180C CTL1 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; CTL1 has a paralog, CET1, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 4967 YMR181C YMR181C Protein of unknown function Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication 4968 YMR182C RGM1 Putative zinc finger DNA binding transcription factor Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication C IDA; IDA; IDA cytosol; nucleus; nuclear chromatin GO_0005829; GO_0005634; GO_0000790 4969 YMR182W-A MIN3 Putative protein of unknown function Putative protein of unknown function 4970 YMR183C SSO2 Plasma membrane t-SNARE Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication C IDA; HDA; HDA; HDA; IDA plasma membrane; cytoplasm; endoplasmic reticulum; cell periphery; prospore membrane GO_0005886; GO_0005737; GO_0005783; GO_0071944; GO_0005628 4971 YMR184W ADD37 Protein of unknown function Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 4972 YMR185W RTP1 Protein required for the nuclear import and biogenesis of RNA pol II Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene C IDA intracellular GO_0005622 4973 YMR186W HSC82 Cytoplasmic chaperone of the Hsp90 family Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication C HDA; HDA; HDA mitochondrion; cytoplasm; plasma membrane GO_0005739; GO_0005737; GO_0005886 4974 YMR187C YMR187C Putative protein of unknown function Putative protein of unknown function; YMR187C is not an essential gene 4975 YMR188C MRPS17 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 4976 YMR189W GCV2 P subunit of the mitochondrial glycine decarboxylase complex P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm C HDA mitochondrion GO_0005739 4977 YMR190C SGS1 RecQ family nucleolar DNA helicase RecQ family nucleolar DNA helicase; role in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; stimulates DNA catenation/decatenation activity of Top3p; potential repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress C HDA; IPI; IDA nucleus; RecQ helicase-Topo III complex; nucleolus GO_0005634; GO_0031422; GO_0005730 4978 YMR191W SPG5 Protein required for proteasome assembly during quiescence Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources 4979 YMR192W GYL1 Putative GTPase activating protein (GAP) with a role in exocytosis Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication C HDA; HDA; HDA; HDA; IDA; HDA; IDA; IDA cytoplasm; cellular bud neck; mitochondrion; cellular bud; incipient cellular bud site; cellular bud tip; plasma membrane; Golgi-associated vesicle GO_0005737; GO_0005935; GO_0005739; GO_0005933; GO_0000131; GO_0005934; GO_0005886; GO_0005798 4980 YMR193C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4981 YMR193W MRPL24 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 4982 YMR194C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 4983 YMR194C-B CMC4 Protein that localizes to the mitochondrial intermembrane space Protein that localizes to the mitochondrial intermembrane space; localizes via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs C IDA mitochondrial intermembrane space GO_0005758 4984 YMR194W RPL36A Ribosomal 60S subunit protein L36A Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 4985 YMR195W ICY1 Protein of unknown function Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ICY2, that arose from the whole genome duplication C HDA fungal-type vacuole membrane GO_0000329 4986 YMR196W YMR196W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene C HDA cytoplasm GO_0005737 4987 YMR197C VTI1 Protein involved in cis-Golgi membrane traffic Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways C IPI; HDA; IDA SNARE complex; fungal-type vacuole membrane; integral component of Golgi membrane GO_0031201; GO_0000329; GO_0030173 4988 YMR198W CIK1 Kinesin-associated protein Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA spindle pole body; kinesin complex; nucleus; spindle GO_0005816; GO_0005871; GO_0005634; GO_0005819 4989 YMR199W CLN1 G1 cyclin involved in regulation of the cell cycle G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 4990 YMR200W ROT1 Molecular chaperone involved in protein folding in ER Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface C IDA; HDA endoplasmic reticulum membrane; endoplasmic reticulum GO_0005789; GO_0005783 4991 YMR201C RAD14 Protein that recognizes and binds damaged DNA during NER Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair C IPI; IDA nucleotide-excision repair factor 1 complex; nucleus GO_0000110; GO_0005634 4992 YMR202W ERG2 C-8 sterol isomerase C-8 sterol isomerase; catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis C HDA endoplasmic reticulum GO_0005783 4993 YMR203W TOM40 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore; pre-Tom40p is phosphorylated by PKA, which impairs its import into mitochondria under non-respiratory conditions C HDA; IDA; HDA mitochondrion; mitochondrial outer membrane translocase complex; mitochondrial outer membrane GO_0005739; GO_0005742; GO_0005741 4994 YMR204C INP1 Peripheral membrane protein of peroxisomes Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane C IDA; IDA extrinsic component of intraperoxisomal membrane; peroxisome GO_0005780; GO_0005777 4995 YMR205C PFK2 Beta subunit of heterooctameric phosphofructokinase Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes C IPI; IDA; IDA; HDA vacuolar proton-transporting V-type ATPase complex; 6-phosphofructokinase complex; mitochondrion; cytoplasm GO_0016471; GO_0005945; GO_0005739; GO_0005737 4996 YMR206W YMR206W Putative protein of unknown function Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication 4997 YMR207C HFA1 Mitochondrial acetyl-coenzyme A carboxylase Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; HFA1 has a paralog, ACC1, that arose from the whole genome duplication C IDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 4998 YMR208W ERG12 Mevalonate kinase Mevalonate kinase; acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate C HDA; HDA; HDA cytoplasm; nucleus; cytosol GO_0005737; GO_0005634; GO_0005829 4999 YMR209C YMR209C Putative S-adenosylmethionine-dependent methyltransferase Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene 5000 YMR210W MGL2 Monoacylglycerol and triacylglycerol lipase Putative acyltransferase with similarity to Eeb1p and Eht1p; has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification 5001 YMR211W DML1 Essential protein involved in mtDNA inheritance Essential protein involved in mtDNA inheritance; may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 5002 YMR212C EFR3 Protein required for Stt4-containing PI kinase complex localization Protein required for Stt4-containing PI kinase complex localization; required for Stt4-containing phosphoinositide (PI) kinase patch assembly at the plasma membrane; recruited to the plasma membrane via a conserved basic patch near its N-terminus; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene C HDA; HDA; IDA mitochondrion; cell periphery; plasma membrane GO_0005739; GO_0071944; GO_0005886 5003 YMR213W CEF1 Essential splicing factor Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p C IDA; IDA Prp19 complex; U2-type catalytic step 1 spliceosome GO_0000974; GO_0071006 5004 YMR214W SCJ1 One of several homologs of bacterial chaperone DnaJ One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins C HDA; IDA endoplasmic reticulum; endoplasmic reticulum lumen GO_0005783; GO_0005788 5005 YMR215W GAS3 Putative 1,3-beta-glucanosyltransferase Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation C IDA; IDA; HDA plasma membrane; fungal-type cell wall; fungal-type vacuole GO_0005886; GO_0009277; GO_0000324 5006 YMR216C SKY1 SR protein kinase (SRPK) SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 C IDA cytoplasm GO_0005737 5007 YMR217W GUA1 GMP synthase GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA 5008 YMR218C TRS130 Component of transport protein particle (TRAPP) complex II Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic C IDA; IDA; IDA early endosome; trans-Golgi network; TRAPPII protein complex GO_0005769; GO_0005802; GO_1990071 5009 YMR219W ESC1 Protein involved in telomeric silencing Protein localized to the nuclear periphery; involved in telomeric silencing; interacts with PAD4-domain of Sir4p C IDA; IDA nuclear chromosome, telomeric region; nuclear periphery GO_0000784; GO_0034399 5010 YMR220W ERG8 Phosphomevalonate kinase Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5011 YMR221C FMP42 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy C HDA; HDA; HDA fungal-type vacuole membrane; mitochondrion; fungal-type vacuole GO_0000329; GO_0005739; GO_0000324 5012 YMR222C FSH2 Putative serine hydrolase that localizes to the cytoplasm Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 C HDA cytoplasm GO_0005737 5013 YMR223W UBP8 Ubiquitin-specific protease component of the SAGA acetylation complex Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B C IDA; IDA; IDA SAGA complex; DUBm complex; SLIK (SAGA-like) complex GO_0000124; GO_0071819; GO_0046695 5014 YMR224C MRE11 Nuclease subunit of the MRX complex with Rad50p and Xrs2p Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress C HDA; HDA; IPI nucleus; mitochondrion; Mre11 complex GO_0005634; GO_0005739; GO_0030870 5015 YMR225C MRPL44 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 5016 YMR226C YMR226C NADP(+)-dependent serine dehydrogenase and carbonyl reductase NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments C HDA; HDA; HDA cytoplasm; ribosome; nucleus GO_0005737; GO_0005840; GO_0005634 5017 YMR227C TAF7 TFIID subunit (67 kDa) TFIID subunit (67 kDa); involved in RNA polymerase II transcription initiation C HDA; IDA nucleus; transcription factor TFIID complex GO_0005634; GO_0005669 5018 YMR228W MTF1 Mitochondrial RNA polymerase specificity factor Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation C IDA; IDA; HDA; IDA mitochondrial intermembrane space; mitochondrial DNA-directed RNA polymerase complex; mitochondrion; mitochondrial matrix GO_0005758; GO_0034245; GO_0005739; GO_0005759 5019 YMR229C RRP5 RNA binding protein involved in synthesis of 18S and 5.8S rRNAs RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress C HDA; HDA; IDA nucleolus; 90S preribosome; small-subunit processome GO_0005730; GO_0030686; GO_0032040 5020 YMR230W RPS10B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication 5021 YMR230W-A YMR230W-A Putative protein of unknown function Putative protein of unknown function 5022 YMR231W PEP5 Histone E3 ligase, component of CORVET membrane tethering complex Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones C IDA; IDA; IPI CORVET complex; fungal-type vacuole membrane; HOPS complex GO_0033263; GO_0000329; GO_0030897 5023 YMR232W FUS2 Cell fusion regulator Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p C HDA; HDA; IDA; IDA mitochondrion; nucleus; cytoplasm; mating projection tip GO_0005739; GO_0005634; GO_0005737; GO_0043332 5024 YMR233W TRI1 Non-essential sumoylated protein of unknown function Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication C HDA; HDA; HDA nucleus; nucleolus; cytoplasm GO_0005634; GO_0005730; GO_0005737 5025 YMR234W RNH1 Ribonuclease H1 Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I. C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5026 YMR235C RNA1 GTPase activating protein (GAP) for Gsp1p GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 5027 YMR236W TAF9 Subunit (17 kDa) of TFIID and SAGA complexes Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 C IDA; IDA; IDA SAGA complex; transcription factor TFIID complex; SLIK (SAGA-like) complex GO_0000124; GO_0005669; GO_0046695 5028 YMR237W BCH1 Member of the ChAPs family (Chs5p-Arf1p-binding proteins) Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication C HDA; HDA; IDA clathrin-coated vesicle; ribosome; exomer complex GO_0030136; GO_0005840; GO_0034044 5029 YMR238W DFG5 Putative mannosidase Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p C HDA endoplasmic reticulum GO_0005783 5030 YMR239C RNT1 Nuclear dsRNA-specific ribonuclease (RNase III) Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes C IDA; IDA nucleoplasm; nucleolus GO_0005654; GO_0005730 5031 YMR240C CUS1 Protein required for assembly of U2 snRNP into the spliceosome Protein required for assembly of U2 snRNP into the spliceosome; forms a complex with Hsh49p and Hsh155p C IDA; IDA U2-type prespliceosome; U2 snRNP GO_0071004; GO_0005686 5032 YMR241W YHM2 Citrate and oxoglutarate carrier protein Citrate and oxoglutarate carrier protein; exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome C IDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 5033 YMR242C RPL20A Ribosomal 60S subunit protein L20A Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 5034 YMR242W-A YMR242W-A Putative protein of unknown function Putative protein of unknown function 5035 YMR243C ZRC1 Vacuolar membrane zinc transporter Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication C IDA; HDA fungal-type vacuole; fungal-type vacuole membrane GO_0000324; GO_0000329 5036 YMR244C-A COA6 Protein involved in cytochrome c oxidase (Complex IV) assembly Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA; IDA nucleus; cytoplasm; mitochondrion; mitochondrial intermembrane space GO_0005634; GO_0005737; GO_0005739; GO_0005758 5037 YMR244W YMR244W Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole GO_0000324 5038 YMR245W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5039 YMR246W FAA4 Long chain fatty acyl-CoA synthetase Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA lipid particle; cytoplasmic stress granule; cytoplasm; plasma membrane GO_0005811; GO_0010494; GO_0005737; GO_0005886 5040 YMR247C RKR1 RING domain E3 ubiquitin ligase RING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins; component of ribosome-bound RQC (ribosome quality control) complex required for degradation of polypeptides arising from stalled translation; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration C HDA; IDA; IDA ribosome; RQC complex; nucleus GO_0005840; GO_1990112; GO_0005634 5041 YMR247W-A YMR247W-A Putative protein of unknown function Putative protein of unknown function C HDA fungal-type vacuole GO_0000324 5042 YMR250W GAD1 Glutamate decarboxylase Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress C HDA cytoplasm GO_0005737 5043 YMR251W GTO3 Omega class glutathione transferase Omega class glutathione transferase; putative cytosolic localization C IDA cytoplasm GO_0005737 5044 YMR251W-A HOR7 Protein of unknown function Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication C HDA; HDA; HDA; IDA cell periphery; endoplasmic reticulum; fungal-type vacuole; plasma membrane GO_0071944; GO_0005783; GO_0000324; GO_0005886 5045 YMR252C MLO1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene C HDA mitochondrion GO_0005739 5046 YMR253C YMR253C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene C HDA cytoplasm GO_0005737 5047 YMR254C YMR254C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5048 YMR255W GFD1 Coiled-coiled protein of unknown function Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress C IDA; IDA nuclear pore; cytoplasm GO_0005643; GO_0005737 5049 YMR256C COX7 Subunit VII of cytochrome c oxidase (Complex IV) Subunit VII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain C HDA; IPI mitochondrion; mitochondrial respiratory chain complex IV GO_0005739; GO_0005751 5050 YMR257C PET111 Mitochondrial translational activator specific for the COX2 mRNA Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5051 YMR258C ROY1 GTPase inhibitor with similarity to F-box proteins GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p C HDA; HDA; HDA cytoplasm; endoplasmic reticulum; nucleus GO_0005737; GO_0005783; GO_0005634 5052 YMR259C TRM732 Protein involved in 2'-O-methylation of C32 of substrate tRNAs Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene C HDA cytoplasm GO_0005737 5053 YMR260C TIF11 Translation initiation factor eIF1A Translation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 C IDA; IDA; IDA eukaryotic 43S preinitiation complex; cytoplasmic stress granule; eukaryotic 48S preinitiation complex GO_0016282; GO_0010494; GO_0033290 5054 YMR261C TPS3 Regulatory subunit of trehalose-6-phosphate synthase/phosphatase Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication C HDA; IPI cytoplasm; alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) GO_0005737; GO_0005946 5055 YMR262W YMR262W Protein of unknown function Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene 5056 YMR263W SAP30 Component of Rpd3L histone deacetylase complex Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance C IDA; IPI; HDA Rpd3L complex; histone deacetylase complex; Rpd3L-Expanded complex GO_0033698; GO_0000118; GO_0070210 5057 YMR264W CUE1 Ubiquitin-binding protein Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane; Doa10p ubiquitin ligase complex; mitochondrion; Hrd1p ubiquitin ligase ERAD-L complex GO_0005783; GO_0030176; GO_0000837; GO_0005739; GO_0000839 5058 YMR265C YMR265C Putative protein of unknown function Putative protein of unknown function 5059 YMR266W RSN1 Membrane protein of unknown function Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane C HDA; HDA; HDA; IDA cell periphery; fungal-type vacuole membrane; endoplasmic reticulum; membrane GO_0071944; GO_0000329; GO_0005783; GO_0016020 5060 YMR267W PPA2 Mitochondrial inorganic pyrophosphatase Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate C HDA mitochondrion GO_0005739 5061 YMR268C PRP24 Splicing factor that reanneals snRNPs during spliceosome recycling Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs C IDA U6 snRNP GO_0005688 5062 YMR269W TMA23 Nucleolar protein implicated in ribosome biogenesis Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan C HDA; HDA nucleolus; ribosome GO_0005730; GO_0005840 5063 YMR270C RRN9 Protein involved in promoting high level transcription of rDNA Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I C IDA; IDA nucleolus; RNA polymerase I upstream activating factor complex GO_0005730; GO_0000500 5064 YMR271C URA10 Minor orotate phosphoribosyltransferase (OPRTase) isozyme Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 5065 YMR272C SCS7 Sphingolipid alpha-hydroxylase Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth C HDA endoplasmic reticulum GO_0005783 5066 YMR272W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SCS7/YMR272C 5067 YMR272W-B YMR272W-B Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching C HDA fungal-type vacuole GO_0000324 5068 YMR273C ZDS1 Protein with a role in regulating Swe1p-dependent polarized growth Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication C IDA; IDA; IDA; IPI cellular bud tip; cellular bud neck; incipient cellular bud site; cytoplasm GO_0005934; GO_0005935; GO_0000131; GO_0005737 5069 YMR274C RCE1 Type II CAAX prenyl protease Type II CAAX prenyl protease; involved in the proteolysis and maturation of Ras and the a-factor mating pheromone C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 5070 YMR275C BUL1 Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication C IPI; IDA plasma membrane; ubiquitin ligase complex GO_0005886; GO_0000151 5071 YMR276W DSK2 Nuclear-enriched ubiquitin-like polyubiquitin-binding protein Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress 5072 YMR277W FCP1 Carboxy-terminal domain (CTD) phosphatase Carboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 5073 YMR278W PRM15 Phosphoribomutase Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5074 YMR279C YMR279C Putative boron transporter involved in boron efflux and resistance Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication C HDA cell periphery GO_0071944 5075 YMR280C CAT8 Zinc cluster transcriptional activator Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5076 YMR281W GPI12 ER membrane protein involved in the second step of GPI anchor assembly ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol C HDA; HDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 5077 YMR282C AEP2 Mitochondrial protein Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader C HDA mitochondrion GO_0005739 5078 YMR283C RIT1 Initiator methionine 2'-O-ribosyl phosphate transferase Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA C HDA cytoplasm GO_0005737 5079 YMR284W YKU70 Subunit of the telomeric Ku complex (Yku70p-Yku80p) Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair C IDA; IDA Ku70:Ku80 complex; nuclear envelope GO_0043564; GO_0005635 5080 YMR285C NGL2 Protein involved in 5.8S rRNA processing Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication C IDA intracellular GO_0005622 5081 YMR286W MRPL33 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 5082 YMR287C DSS1 3'-5' exoribonuclease 3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs C IDA; HDA mitochondrial degradosome; mitochondrion GO_0045025; GO_0005739 5083 YMR288W HSH155 U2-snRNP associated splicing factor U2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 C IDA; IDA U2 snRNP; U2-type prespliceosome GO_0005686; GO_0071004 5084 YMR289W ABZ2 Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase) Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase); catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis C HDA cytoplasm GO_0005737 5085 YMR290C HAS1 ATP-dependent RNA helicase ATP-dependent RNA helicase; involved in the biogenesis of 40S and 60S ribosome subunits; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles C IDA; IDA; IDA; IDA; HDA; HDA nuclear envelope; nucleolus; box H/ACA snoRNP complex; box C/D snoRNP complex; 90S preribosome; preribosome, large subunit precursor GO_0005635; GO_0005730; GO_0031429; GO_0031428; GO_0030686; GO_0030687 5086 YMR290W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1/YMR290C gene which encodes an ATP-dependent RNA helicase 5087 YMR291W TDA1 Protein kinase of unknown cellular role Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5088 YMR292W GOT1 Homodimeric protein that is packaged into COPII vesicles Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition C IDA; IDA; IDA; IDA; IDA endoplasmic reticulum; Golgi membrane; Golgi cis cisterna; Golgi apparatus; ER to Golgi transport vesicle GO_0005783; GO_0000139; GO_0000137; GO_0005794; GO_0030134 5089 YMR293C HER2 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase C HDA; IDA; IDA mitochondrion; extrinsic component of mitochondrial inner membrane; glutamyl-tRNA(Gln) amidotransferase complex GO_0005739; GO_0031314; GO_0030956 5090 YMR294W JNM1 Component of the yeast dynactin complex Component of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B C IDA; IDA; IDA; IDA astral microtubule; dynactin complex; cell cortex; spindle pole body GO_0000235; GO_0005869; GO_0005938; GO_0005816 5091 YMR294W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents 5092 YMR295C YMR295C Protein of unknown function that associates with ribosomes Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication C HDA; HDA; HDA ribosome; cellular bud; mating projection tip GO_0005840; GO_0005933; GO_0043332 5093 YMR296C LCB1 Component of serine palmitoyltransferase Component of serine palmitoyltransferase; responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine C HDA; IMP; IDA endoplasmic reticulum; serine C-palmitoyltransferase complex; SPOTS complex GO_0005783; GO_0017059; GO_0035339 5094 YMR297W PRC1 Vacuolar carboxypeptidase Y (proteinase C, CPY) Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family C HDA; HDA; IDA; IDA endoplasmic reticulum; cytoplasm; fungal-type vacuole; fungal-type vacuole lumen GO_0005783; GO_0005737; GO_0000324; GO_0000328 5095 YMR298W LIP1 Ceramide synthase subunit Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis C HDA; IDA; IDA; HDA endoplasmic reticulum; endoplasmic reticulum membrane; acyl-CoA ceramide synthase complex; nuclear envelope GO_0005783; GO_0005789; GO_0061576; GO_0005635 5096 YMR299C DYN3 Dynein light intermediate chain (LIC) Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration C IDA; IPI cytoplasmic microtubule; cytoplasmic dynein complex GO_0005881; GO_0005868 5097 YMR300C ADE4 Phosphoribosylpyrophosphate amidotransferase (PRPPAT) Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase C HDA cytoplasm GO_0005737 5098 YMR301C ATM1 Mitochondrial inner membrane ATP-binding cassette (ABC) transporter Mitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 5099 YMR302C YME2 Integral inner mitochondrial membrane protein Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 5100 YMR303C ADH2 Glucose-repressible alcohol dehydrogenase II Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 C HDA cytoplasm GO_0005737 5101 YMR304C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 3' end of verified ORF SCW10/YMR305C 5102 YMR304W UBP15 Ubiquitin-specific protease involved in protein deubiquitination Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p C IDA; HDA; IDA; HDA peroxisome; endoplasmic reticulum; cytosol; cytoplasm GO_0005777; GO_0005783; GO_0005829; GO_0005737 5103 YMR305C SCW10 Cell wall protein with similarity to glucanases Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication C IDA; HDA; HDA; IDA; HDA extracellular region; cytoplasm; endoplasmic reticulum; fungal-type cell wall; cell periphery GO_0005576; GO_0005737; GO_0005783; GO_0009277; GO_0071944 5104 YMR306C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF FKS3/YMR306W 5105 YMR306W FKS3 Protein involved in spore wall assembly Protein involved in spore wall assembly; has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA mitochondrion; endoplasmic reticulum GO_0005739; GO_0005783 5106 YMR307C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GAS1/YMR307W 5107 YMR307W GAS1 Beta-1,3-glucanosyltransferase Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation C IDA; HDA; IDA; IDA; IDA; IDA; IDA; IDA ER to Golgi transport vesicle; mitochondrion; primary cell septum; cellular bud scar; plasma membrane; fungal-type cell wall; nuclear periphery; membrane raft GO_0030134; GO_0005739; GO_0000936; GO_0005621; GO_0005886; GO_0009277; GO_0034399; GO_0045121 5108 YMR308C PSE1 Karyopherin/importin that interacts with the nuclear pore complex Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 5109 YMR309C NIP1 eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3) eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection C IDA; IDA; HDA; IDA cytoplasmic stress granule; eukaryotic translation initiation factor 3 complex; cytoplasm; multi-eIF complex GO_0010494; GO_0005852; GO_0005737; GO_0043614 5110 YMR310C YMR310C Putative methyltransferase Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication C HDA nucleus GO_0005634 5111 YMR311C GLC8 Regulatory subunit of protein phosphatase 1 (Glc7p) Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5112 YMR312W ELP6 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity C IDA Elongator holoenzyme complex GO_0033588 5113 YMR313C TGL3 Bifunctional triacylglycerol lipase and LPE acyltransferase Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation C IDA lipid particle GO_0005811 5114 YMR314W PRE5 Alpha 6 subunit of the 20S proteasome Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress C IDA; IDA proteasome storage granule; proteasome core complex, alpha-subunit complex GO_0034515; GO_0019773 5115 YMR315W YMR315W Protein with NADP(H) oxidoreductase activity Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5116 YMR315W-A YMR315W-A Putative protein of unknown function Putative protein of unknown function 5117 YMR316C-A YMR316C-A Protein of unknown function Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W C HDA endoplasmic reticulum GO_0005783 5118 YMR316C-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 5' end of ORF YMR317W 5119 YMR316W DIA1 Protein of unknown function Protein of unknown function; involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C HDA cytoplasm GO_0005737 5120 YMR317W YMR317W Putative protein of unknown function Putative protein of unknown function; has some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene 5121 YMR318C ADH6 NADPH-dependent medium chain alcohol dehydrogenase NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress 5122 YMR319C FET4 Low-affinity Fe(II) transporter of the plasma membrane Low-affinity Fe(II) transporter of the plasma membrane C IDA; HDA; IDA; HDA integral component of plasma membrane; fungal-type vacuole; plasma membrane; cell periphery GO_0005887; GO_0000324; GO_0005886; GO_0071944 5123 YMR320W YMR320W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5124 YMR321C YMR321C Putative protein of unknown function Putative protein of unknown function; proposed to be a palmitoylated membrane protein; YMR321C has a paralog, SAM4, that arose from a single-locus duplication 5125 YMR322C SNO4 Possible chaperone and cysteine protease Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation 5126 YMR323W ERR3 Enolase, a phosphopyruvate hydratase Enolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant in glucose C HDA cytoplasm GO_0005737 5127 YMR324C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription is AZF1 dependent in glycerol-lactate medium and SLT2 dependent in response to the lipid hydroperoxides 5128 YMR325W PAU19 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions 5129 YMR326C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 5130 YNL001W DOM34 Protein that facilitates ribosomal subunit dissociation Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication C HDA; IDA cytoplasm; Dom34-Hbs1 complex GO_0005737; GO_1990533 5131 YNL002C RLP7 Nucleolar protein similar to large ribosomal subunit L7 proteins Nucleolar protein similar to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs; binds junction of ITS2 and ITS2-proximal stem between the 3' end of 5.8S rRNA and the 5' end of 25S rRNA C IDA; IDA preribosome, large subunit precursor; nucleolus GO_0030687; GO_0005730 5132 YNL003C PET8 S-adenosylmethionine transporter of the mitochondrial inner membrane S-adenosylmethionine transporter of the mitochondrial inner membrane; member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth C HDA mitochondrion GO_0005739 5133 YNL004W HRB1 Poly(A+) RNA-binding protein Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 5134 YNL005C MRP7 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 5135 YNL006W LST8 Protein required for the transport of Gap1p Protein required for the transport of Gap1p; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat C IDA; IDA; IPI; IDA; IDA; IPI; HDA endosome membrane; nuclear periphery; TORC2 complex; extrinsic component of cytoplasmic side of plasma membrane; Golgi membrane; TORC1 complex; cytosol GO_0010008; GO_0034399; GO_0031932; GO_0031234; GO_0000139; GO_0031931; GO_0005829 5136 YNL007C SIS1 Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1 C IDA; IDA nucleus; cytosolic small ribosomal subunit GO_0005634; GO_0022627 5137 YNL008C ASI3 Subunit of the inner nuclear membrane Asi ubiquitin ligase complex Subunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI3 has a paralog, ASI1, that arose from the whole genome duplication C HDA; IDA; IDA nuclear periphery; nuclear inner membrane; Asi complex GO_0034399; GO_0005637; GO_0097658 5138 YNL009W IDP3 Peroxisomal NADP-dependent isocitrate dehydrogenase Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication C IDA; IDA; HDA; IDA cytoplasm; peroxisomal importomer complex; mitochondrion; peroxisome GO_0005737; GO_1990429; GO_0005739; GO_0005777 5139 YNL010W YNL010W Putative protein of unknown function Putative protein of unknown function; similar to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5140 YNL011C YNL011C Putative protein of unknown function Putative protein of unknown function; YNL011C is not an essential gene 5141 YNL012W SPO1 Meiosis-specific prospore protein Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B C IDA; HDA; IDA; IDA nucleus; fungal-type vacuole; prospore membrane; endoplasmic reticulum GO_0005634; GO_0000324; GO_0005628; GO_0005783 5142 YNL013C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W 5143 YNL014W HEF3 Translational elongation factor EF-3 Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication C IPI cytosolic ribosome GO_0022626 5144 YNL015W PBI2 Cytosolic inhibitor of vacuolar proteinase B (PRB1) Cytosolic inhibitor of vacuolar proteinase B (PRB1); required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion; protein abundance increases in response to DNA replication stress C IPI; HDA; HDA; HDA fungal-type vacuole; cytoplasm; cytosol; nucleus GO_0000324; GO_0005737; GO_0005829; GO_0005634 5145 YNL016W PUB1 Poly (A)+ RNA-binding protein Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress C IDA; IDA; IDA; IDA cytoplasmic stress granule; cytoplasm; nucleus; P-body GO_0010494; GO_0005737; GO_0005634; GO_0000932 5146 YNL017C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the tRNA ORF tI(AAU)N2 5147 YNL018C YNL018C Putative protein of unknown function Putative protein of unknown function; YNL018C has a paralog, YNL034W, that arose from a segmental duplication C HDA prospore membrane GO_0005628 5148 YNL019C YNL019C Putative protein of unknown function Putative protein of unknown function; expression induced during heat stress; YNL019C has a paralog, YNL033W, that arose from a segmental duplication C HDA; HDA prospore membrane; fungal-type vacuole GO_0005628; GO_0000324 5149 YNL020C ARK1 Serine/threonine protein kinase Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication C IDA; HDA actin cortical patch; mating projection tip GO_0030479; GO_0043332 5150 YNL021W HDA1 Putative catalytic subunit of a class II histone deacetylase complex Putative catalytic subunit of a class II histone deacetylase complex; role in azole resistance via Hsp90p, and in the heat shock response; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; other members of the HDA1 histone deacetylase complex are Hda2p and Hda3p C IPI HDA1 complex GO_0070823 5151 YNL022C RCM1 rRNA m5C methyltransferase rRNA m5C methyltransferase; methylates cytosine at position 2278 of 25S rRNA while Nop2p methylates cytosine at position 2870; contains seven beta-strand methyltransferase motif; localized to the nucleolus; interacts with Trm112p; homolog of NSUN5A, a human gene which is deleted in Williams-Beuren Syndrome C IDA; IDA nucleus; nucleolus GO_0005634; GO_0005730 5152 YNL023C FAP1 Protein that binds to Fpr1p Protein that binds to Fpr1p; confers rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 C IDA cytoplasm GO_0005737 5153 YNL024C EFM6 Putative S-adenosylmethionine-dependent lysine methyltransferase Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern C HDA cytoplasm GO_0005737 5154 YNL024C-A KSH1 Essential protein suggested to function early in the secretory pathway Essential protein suggested to function early in the secretory pathway; inviability is suppressed by overexpression of Golgi protein Tvp23p; ortholog of human Kish 5155 YNL025C SSN8 Cyclin-like component of the RNA polymerase II holoenzyme Cyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C C IDA mediator complex GO_0016592 5156 YNL026W SAM50 Component of the Sorting and Assembly Machinery (SAM) complex Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 C HDA; IDA; IDA; HDA mitochondrion; integral component of mitochondrial outer membrane; mitochondrial sorting and assembly machinery complex; mitochondrial outer membrane GO_0005739; GO_0031307; GO_0001401; GO_0005741 5157 YNL027W CRZ1 Transcription factor, activates transcription of stress response genes Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 5158 YNL028W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5159 YNL029C KTR5 Putative mannosyltransferase involved in protein glycosylation Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR5 has a paralog, KTR7, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5160 YNL030W HHF2 Histone H4 Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity C IDA replication fork protection complex GO_0031298 5161 YNL031C HHT2 Histone H3 Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage C IDA; IDA CENP-A containing nucleosome; replication fork protection complex GO_0043505; GO_0031298 5162 YNL032W SIW14 Tyrosine phosphatase involved in actin organization and endocytosis Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm C HDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 5163 YNL033W YNL033W Putative protein of unknown function Putative protein of unknown function; YNL033W has a paralog, YNL019C, that arose from a segmental duplication 5164 YNL034W YNL034W Putative protein of unknown function Putative protein of unknown function; not an essential gene; YNL034W has a paralog, YNL018C, that arose from a segmental duplication 5165 YNL035C YNL035C Nuclear protein of unknown function Nuclear protein of unknown function; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress C IDA; HDA cytosol; nucleus GO_0005829; GO_0005634 5166 YNL036W NCE103 Carbonic anhydrase Carbonic anhydrase; metalloenzyme that catalyzes CO2 hydration to bicarbonate, which is an important metabolic substrate, and protons; not expressed under conditions of high CO2, such as inside a growing colony, but transcription is induced in response to low CO2 levels, such as on the colony surface in ambient air; poorly transcribed under aerobic conditions and at an undetectable level under anaerobic conditions; abundance increases in response to DNA replication stress C HDA; HDA; IDA nucleus; cytoplasm; mitochondrial intermembrane space GO_0005634; GO_0005737; GO_0005758 5167 YNL037C IDH1 Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle C HDA; IDA; IDA; IDA mitochondrion; mitochondrial isocitrate dehydrogenase complex (NAD+); mitochondrial matrix; mitochondrial nucleoid GO_0005739; GO_0005962; GO_0005759; GO_0042645 5168 YNL038W GPI15 Protein involved in the synthesis of GlcNAc-PI Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol 5169 YNL039W BDP1 Essential subunit of RNA polymerase III transcription factor (TFIIIB) Essential subunit of RNA polymerase III transcription factor (TFIIIB); TFIIIB is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs C IDA transcription factor TFIIIB complex GO_0000126 5170 YNL040W YNL040W Protein of unknown function Putative protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene C HDA cytoplasm GO_0005737 5171 YNL041C COG6 Component of the conserved oligomeric Golgi complex Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C IMP Golgi transport complex GO_0017119 5172 YNL042W BOP3 Protein of unknown function Protein of unknown function; potential Cdc28p substrate; overproduction confers resistance to methylmercury C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5173 YNL042W-B YNL042W-B Putative protein of unknown function Putative protein of unknown function 5174 YNL043C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W 5175 YNL044W YIP3 Protein localized to COPII vesicles Protein localized to COPII vesicles; proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p C IDA; IDA; IDA Golgi apparatus; ER to Golgi transport vesicle; endoplasmic reticulum GO_0005794; GO_0030134; GO_0005783 5176 YNL045W LAP2 Leucyl aminopeptidase yscIV with epoxide hydrolase activity Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5177 YNL046W YNL046W Putative protein of unknown function Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) C HDA; HDA endoplasmic reticulum; nucleus GO_0005783; GO_0005634 5178 YNL047C SLM2 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 5179 YNL048W ALG11 Alpha-1,2-mannosyltransferase Alpha-1,2-mannosyltransferase; catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER C IDA endoplasmic reticulum membrane GO_0005789 5180 YNL049C SFB2 Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication C IPI COPII vesicle coat GO_0030127 5181 YNL050C YNL050C Putative protein of unknown function Putative protein of unknown function; YNL050c is not an essential gene 5182 YNL051W COG5 Component of the conserved oligomeric Golgi complex Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C HDA; IMP cytosol; Golgi transport complex GO_0005829; GO_0017119 5183 YNL052W COX5A Subunit Va of cytochrome c oxidase Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication C IPI; HDA mitochondrial respiratory chain complex IV; mitochondrion GO_0005751; GO_0005739 5184 YNL053W MSG5 Dual-specificity protein phosphatase Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 5185 YNL054W VAC7 Integral vacuolar membrane protein Integral vacuolar membrane protein; involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock C IDA; IDA; HDA; IDA; HDA PAS complex; integral component of membrane; fungal-type vacuole membrane; vacuolar membrane; cytoplasm GO_0070772; GO_0016021; GO_0000329; GO_0005774; GO_0005737 5186 YNL054W-A YNL054W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 5187 YNL055C POR1 Mitochondrial porin (voltage-dependent anion channel) Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication C IPI; HDA; IDA; HDA mitochondrion; mitochondrial outer membrane; integral component of mitochondrial outer membrane; cytoplasm GO_0005739; GO_0005741; GO_0031307; GO_0005737 5188 YNL056W OCA2 Protein of unknown function Putative protein of unknown function; similar to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene C HDA cytoplasm GO_0005737 5189 YNL057W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5190 YNL058C YNL058C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuole; not an essential gene; YNL058C has a paralog, PRM5, that arose from the whole genome duplication C HDA; HDA endoplasmic reticulum; fungal-type vacuole GO_0005783; GO_0000324 5191 YNL059C ARP5 Nuclear actin-related protein involved in chromatin remodeling Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; promotes nucleosome shifts in the 3 prime direction C IDA; IDA Ino80 complex; nucleus GO_0031011; GO_0005634 5192 YNL061W NOP2 rRNA m5C methyltransferase rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA while Rcm1p methylates cytosine at position 2278; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; homolog of p120/NSUN1, a human gene upregulated in cancer C HDA; HDA; IDA nucleus; nucleolus; preribosome, large subunit precursor GO_0005634; GO_0005730; GO_0030687 5193 YNL062C GCD10 Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression C IPI; IDA tRNA (m1A) methyltransferase complex; nucleus GO_0031515; GO_0005634 5194 YNL063W MTQ1 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene C HDA mitochondrion GO_0005739 5195 YNL064C YDJ1 Type I HSP40 co-chaperone Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family C IDA; IDA; IDA TRC complex; perinuclear region of cytoplasm; cytosol GO_0072380; GO_0048471; GO_0005829 5196 YNL065W AQR1 Plasma membrane transporter of the major facilitator superfamily Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress C HDA; HDA; HDA; HDA fungal-type vacuole; cell periphery; plasma membrane; cytoplasm GO_0000324; GO_0071944; GO_0005886; GO_0005737 5197 YNL066W SUN4 Cell wall protein related to glucanases Cell wall protein related to glucanases; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication C IDA fungal-type cell wall GO_0009277 5198 YNL067W RPL9B Ribosomal 60S subunit protein L9B Ribosomal 60S subunit protein L9B; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9B has a paralog, RPL9A, that arose from a single-locus duplication C IDA cytosolic large ribosomal subunit GO_0022625 5199 YNL067W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5200 YNL067W-B YNL067W-B Putative protein of unknown function Putative protein of unknown function 5201 YNL068C FKH2 Forkhead family transcription factor Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; facilitates clustering and activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; relocalizes to the cytosol in response to hypoxia; FKH2 has a paralog, FKH1, that arose from the whole genome duplication C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 5202 YNL069C RPL16B Ribosomal 60S subunit protein L16B Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 5203 YNL070W TOM7 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex C IDA; HDA; HDA mitochondrial outer membrane translocase complex; mitochondrion; mitochondrial outer membrane GO_0005742; GO_0005739; GO_0005741 5204 YNL071W LAT1 Dihydrolipoamide acetyltransferase component (E2) of the PDC Dihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA C HDA; IPI mitochondrion; mitochondrial pyruvate dehydrogenase complex GO_0005739; GO_0005967 5205 YNL072W RNH201 Ribonuclease H2 catalytic subunit Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome C IDA; IDA ribonuclease H2 complex; nucleus GO_0032299; GO_0005634 5206 YNL073W MSK1 Mitochondrial lysine-tRNA synthetase Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) C HDA mitochondrion GO_0005739 5207 YNL074C MLF3 Serine-rich protein of unknown function Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication C IDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 5208 YNL075W IMP4 Component of the SSU processome Component of the SSU processome; SSU processome is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs C HDA; IDA; IDA 90S preribosome; small-subunit processome; Mpp10 complex GO_0030686; GO_0032040; GO_0034457 5209 YNL076W MKS1 Pleiotropic negative transcriptional regulator Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling C IDA cytoplasm GO_0005737 5210 YNL077W APJ1 Chaperone with a role in SUMO-mediated protein degradation Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress C HDA; HDA; HDA; IDA mitochondrion; nucleus; cytoplasm; nuclear periphery GO_0005739; GO_0005634; GO_0005737; GO_0034399 5211 YNL078W NIS1 Protein localized in the bud neck at G2/M phase Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network C IDA; IDA cellular bud neck; nucleus GO_0005935; GO_0005634 5212 YNL079C TPM1 Major isoform of tropomyosin Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication C IDA; IDA cellular bud neck contractile ring; actin filament bundle GO_0000142; GO_0032432 5213 YNL080C EOS1 Protein involved in N-glycosylation Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene C HDA; IDA endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 5214 YNL081C SWS2 Putative mitochondrial ribosomal protein of the small subunit Putative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2 5215 YNL082W PMS1 ATP-binding protein required for mismatch repair ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL C HDA; IPI; HDA cytoplasm; MutLalpha complex; nucleus GO_0005737; GO_0032389; GO_0005634 5216 YNL083W SAL1 ADP/ATP transporter ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains C HDA mitochondrion GO_0005739 5217 YNL084C END3 EH domain-containing protein involved in endocytosis EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p C HDA; HDA cell periphery; mating projection tip GO_0071944; GO_0043332 5218 YNL085W MKT1 Protein similar to nucleases that forms a complex with Pbp1p Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains C IDA; HDA; IDA; IDA; IDA P-body; cytoplasm; polysome; cytoplasmic stress granule; nuclear periphery GO_0000932; GO_0005737; GO_0005844; GO_0010494; GO_0034399 5219 YNL086W SNN1 Subunit of the BLOC-1 complex involved in endosomal maturation Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes C IDA; HDA BLOC-1 complex; endosome GO_0031083; GO_0005768 5220 YNL087W TCB2 ER protein involved in ER-plasma membrane tethering ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication C HDA; IDA; IDA; HDA cell periphery; cellular bud; cortical endoplasmic reticulum; endoplasmic reticulum GO_0071944; GO_0005933; GO_0032541; GO_0005783 5221 YNL088W TOP2 Topoisomerase II Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation C IDA; HDA; IPI; HDA synaptonemal complex; nucleus; DNA replication termination region; mitochondrion GO_0000795; GO_0005634; GO_0097047; GO_0005739 5222 YNL089C Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins 5223 YNL090W RHO2 Non-essential small GTPase of the Rho/Rac family of Ras-like proteins Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity and in microtubule assembly C IDA; HDA membrane; cell periphery GO_0016020; GO_0071944 5224 YNL091W NST1 Protein of unknown function Protein of unknown function; mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 C HDA cytoplasm GO_0005737 5225 YNL092W YNL092W S-adenosylmethionine-dependent protein methyltransferase Putative S-adenosylmethionine-dependent methyltransferase; of the seven beta-strand family; YNL092W is not an essential gene 5226 YNL093W YPT53 Stress-induced Rab family GTPase Stress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication C IMP late endosome GO_0005770 5227 YNL094W APP1 Phosphatidate phosphatase, converts phosphatidate to diacylglycerol Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway C IDA; IPI actin cortical patch; actin cytoskeleton GO_0030479; GO_0015629 5228 YNL095C YNL095C Putative protein of unknown function Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5229 YNL096C RPS7B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA small-subunit processome GO_0032040 5230 YNL097C PHO23 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 C IDA; IDA; HDA; HDA Rpd3L complex; histone deacetylase complex; Rpd3L-Expanded complex; nucleus GO_0033698; GO_0000118; GO_0070210; GO_0005634 5231 YNL097C-B YNL097C-B Putative protein of unknown function Putative protein of unknown function 5232 YNL097W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene PHO23/YNL097C 5233 YNL098C RAS2 GTP-binding protein GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication C IDA; IDA; IDA; HDA; IDA plasma membrane; endoplasmic reticulum membrane; nucleus; cell periphery; mitochondrion GO_0005886; GO_0005789; GO_0005634; GO_0071944; GO_0005739 5234 YNL099C OCA1 Putative protein tyrosine phosphatase Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA C HDA cytoplasm GO_0005737 5235 YNL100W MIC27 Component of the MICOS complex Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane C HDA; IDA; IDA; IDA mitochondrion; mitochondrial crista junction; MICOS complex; fungal-type vacuole membrane GO_0005739; GO_0044284; GO_0061617; GO_0000329 5236 YNL101W AVT4 Vacuolar transporter Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters C IDA; IMP; IDA fungal-type vacuole; fungal-type vacuole membrane; vacuole-mitochondrion membrane contact site GO_0000324; GO_0000329; GO_1990816 5237 YNL102W POL1 Catalytic subunit of the DNA polymerase I alpha-primase complex Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis C IDA; IDA; HDA alpha DNA polymerase:primase complex; replication fork; mitochondrion GO_0005658; GO_0005657; GO_0005739 5238 YNL103W MET4 Leucine-zipper transcriptional activator Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p C IDA; IPI Cbf1-Met4-Met28 complex; nucleus GO_0089713; GO_0005634 5239 YNL103W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MET4/YNL104C 5240 YNL104C LEU4 Alpha-isopropylmalate synthase (2-isopropylmalate synthase) Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication C IDA; IDA mitochondrion; cytoplasm GO_0005739; GO_0005737 5241 YNL105W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription 5242 YNL106C INP52 Polyphosphatidylinositol phosphatase Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication C IDA; HDA; IDA actin cortical patch; mating projection tip; cytoplasm GO_0030479; GO_0043332; GO_0005737 5243 YNL107W YAF9 Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain C IDA; HDA; IDA; HDA NuA4 histone acetyltransferase complex; cytoplasm; Swr1 complex; nucleus GO_0035267; GO_0005737; GO_0000812; GO_0005634 5244 YNL108C YNL108C Protein phosphatase Protein phosphatase; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5245 YNL109W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C 5246 YNL110C NOP15 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm C IDA; IDA; IDA nucleolus; nucleus; preribosome, large subunit precursor GO_0005730; GO_0005634; GO_0030687 5247 YNL111C CYB5 Cytochrome b5 Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation C IDA; HDA endoplasmic reticulum membrane; endoplasmic reticulum GO_0005789; GO_0005783 5248 YNL112W DBP2 ATP-dependent RNA helicase of the DEAD-box protein family ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; interacts with YRA1; required for the assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites C HDA; IDA; HDA mitochondrion; nucleus; cytoplasm GO_0005739; GO_0005634; GO_0005737 5249 YNL113W RPC19 RNA polymerase subunit AC19 RNA polymerase subunit AC19; common to RNA polymerases I and III C IDA; IDA DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase III complex GO_0005736; GO_0005666 5250 YNL114C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit 5251 YNL115C YNL115C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene C HDA; HDA fungal-type vacuole; fungal-type vacuole membrane GO_0000324; GO_0000329 5252 YNL116W DMA2 Ubiquitin-protein ligase (E3) Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication C HDA; IMP; IMP cytoplasm; cellular bud tip; cellular bud neck split septin rings GO_0005737; GO_0005934; GO_0032177 5253 YNL117W MLS1 Malate synthase, enzyme of the glyoxylate cycle Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA C HDA; HDA; IDA; IDA cytosol; cytoplasm; peroxisome; peroxisomal matrix GO_0005829; GO_0005737; GO_0005777; GO_0005782 5254 YNL118C DCP2 Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; also enters the nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; IDA; IDA; IDA P-body; nucleus; cytoplasm; Dcp1-Dcp2 complex; cytoplasmic side of membrane GO_0000932; GO_0005634; GO_0005737; GO_0098745; GO_0098562 5255 YNL119W NCS2 Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae C IDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 5256 YNL120C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene 5257 YNL121C TOM70 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication C HDA; IDA; IDA; HDA mitochondrion; integral component of mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrial outer membrane GO_0005739; GO_0031307; GO_0005742; GO_0005741 5258 YNL122C YNL122C Mitochondrial ribosomal protein of the large subunit Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene C IDA; IDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 5259 YNL123W NMA111 Serine protease and general molecular chaperone Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases C IDA nucleus GO_0005634 5260 YNL124W NAF1 RNA-binding protein required for the assembly of box H/ACA snoRNPs RNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p C IDA; IDA nucleoplasm; nucleus GO_0005654; GO_0005634 5261 YNL125C ESBP6 Protein with similarity to monocarboxylate permeases Protein with similarity to monocarboxylate permeases; appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane C HDA; HDA; IDA endoplasmic reticulum; cell periphery; mitochondrion GO_0005783; GO_0071944; GO_0005739 5262 YNL126W SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque C IDA; IDA; IDA gamma-tubulin small complex, spindle pole body; inner plaque of spindle pole body; outer plaque of spindle pole body GO_0000928; GO_0005822; GO_0005824 5263 YNL127W FAR11 Protein involved in recovery from cell cycle arrest Protein involved in recovery from cell cycle arrest; acts in response to pheromone; also involved in regulation of intra-S DNA damage checkpoint and autophagy; is essential for dephosphorylation of Atg13p; interacts with Far3p, Far7p, Far8p, Far9p, Far10p and with the phosphatases Pph21p, Pph22p and Pph3p; has similarity to the N- and C-termini of N. crassa HAM-2; similar to human Fam40A and Fam40B C IDA; HDA; IDA endoplasmic reticulum; cytosol; Golgi trans cisterna GO_0005783; GO_0005829; GO_0000138 5264 YNL128W TEP1 PTEN homolog with no demonstrated inositol lipid phosphatase activity PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 C IDA cytoplasm GO_0005737 5265 YNL129W NRK1 Nicotinamide riboside kinase Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis C HDA; HDA cytoplasm; fungal-type vacuole GO_0005737; GO_0000324 5266 YNL130C CPT1 Cholinephosphotransferase Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication C IDA; IDA; HDA mitochondrial outer membrane; Golgi apparatus; endoplasmic reticulum GO_0005741; GO_0005794; GO_0005783 5267 YNL130C-A DGR1 Protein of unknown function Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose 5268 YNL131W TOM22 Component of the TOM (Translocase of Outer Membrane) complex Component of the TOM (Translocase of Outer Membrane) complex; responsible for initial import of mitochondrially directed proteins; mediates interaction between TOM and TIM complexes and acts as a receptor for precursor proteins C IDA; IDA; HDA; HDA integral component of mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrial outer membrane; mitochondrion GO_0031307; GO_0005742; GO_0005741; GO_0005739 5269 YNL132W KRE33 Protein required for biogenesis of the small ribosomal subunit Protein required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance; essential gene; NAT10, the human homolog, implicated in several types of cancer and premature aging. C HDA; HDA nucleolus; 90S preribosome GO_0005730; GO_0030686 5270 YNL133C FYV6 Protein of unknown function Protein of unknown function; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining C HDA nucleus GO_0005634 5271 YNL134C YNL134C NADH-dependent aldehyde reductase, involved in detoxification of furf Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5272 YNL135C FPR1 Peptidyl-prolyl cis-trans isomerase (PPIase) Peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; N-terminally propionylated in vivo C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 5273 YNL136W EAF7 Subunit of the NuA4 histone acetyltransferase complex Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A C IPI; IDA; HDA NuA4 histone acetyltransferase complex; histone acetyltransferase complex; nucleus GO_0035267; GO_0000123; GO_0005634 5274 YNL137C NAM9 Mitochondrial ribosomal component of the small subunit Mitochondrial ribosomal component of the small subunit C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 5275 YNL138W SRV2 CAP (cyclase-associated protein) CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus, in physically and genetically separate activity, binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis C HDA; IDA mating projection tip; actin cortical patch GO_0043332; GO_0030479 5276 YNL138W-A YSF3 Component of the SF3b subcomplex of the U2 snRNP Component of the SF3b subcomplex of the U2 snRNP; essential protein required for for splicing and for assembly of SF3b C IDA U2 snRNP GO_0005686 5277 YNL139C THO2 Subunit of the THO complex Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids C IPI; HDA; IDA; IDA nucleoplasmic THO complex; nucleus; chromosome, telomeric region; THO complex part of transcription export complex GO_0000446; GO_0005634; GO_0000781; GO_0000445 5278 YNL140C YNL140C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C 5279 YNL141W AAH1 Adenine deaminase (adenine aminohydrolase) Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5280 YNL142W MEP2 Ammonium permease involved in regulation of pseudohyphal growth Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation C IDA plasma membrane GO_0005886 5281 YNL143C YNL143C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels 5282 YNL144C YNL144C Putative protein of unknown function Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; YNL144C has a paralog, YHR131C, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 5283 YNL144W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YNL144C 5284 YNL145W MFA2 Mating pheromone a-factor Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 C IDA; HDA extracellular region; endoplasmic reticulum GO_0005576; GO_0005783 5285 YNL146C-A YNL146C-A Putative protein of unknown function Putative protein of unknown function 5286 YNL146W YNL146W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene C HDA endoplasmic reticulum GO_0005783 5287 YNL147W LSM7 Lsm (Like Sm) protein Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress C IDA; IDA; IDA; IDA; HDA; IDA; IPI cytoplasm; U6 snRNP; U4/U6 x U5 tri-snRNP complex; Lsm1-7-Pat1 complex; nucleus; nucleolus; small nucleolar ribonucleoprotein complex GO_0005737; GO_0005688; GO_0046540; GO_1990726; GO_0005634; GO_0005730; GO_0005732 5288 YNL148C ALF1 Alpha-tubulin folding protein Alpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5289 YNL149C PGA2 Essential protein required for maturation of Gas1p and Pho8p Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect C HDA; HDA endoplasmic reticulum; nuclear envelope GO_0005783; GO_0005635 5290 YNL150W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking 5291 YNL151C RPC31 RNA polymerase III subunit C31 RNA polymerase III subunit C31 C IDA DNA-directed RNA polymerase III complex GO_0005666 5292 YNL152W INN1 Essential protein that associates with contractile actomyosin ring Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis C IPI; HDA; IDA HICS complex; cytoplasm; cellular bud neck contractile ring GO_0044697; GO_0005737; GO_0000142 5293 YNL153C GIM3 Subunit of the heterohexameric cochaperone prefoldin complex Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation C IDA; IPI cytoplasm; prefoldin complex GO_0005737; GO_0016272 5294 YNL154C YCK2 Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK2 has a paralog, YCK1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA plasma membrane; mating projection; cell periphery; cellular bud neck GO_0005886; GO_0005937; GO_0071944; GO_0005935 5295 YNL155W CUZ1 Protein with a role in the ubiquitin-proteasome pathway Protein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5296 YNL156C NSG2 Protein involved in regulation of sterol biosynthesis Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5297 YNL157W IGO1 Protein required for initiation of G0 program Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication C IDA; HDA; IDA nucleus; cytoplasm; P-body GO_0005634; GO_0005737; GO_0000932 5298 YNL158W PGA1 Essential component of GPI-mannosyltransferase II Essential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes C IDA; IPI; HDA integral component of endoplasmic reticulum membrane; mannosyltransferase complex; nuclear envelope GO_0030176; GO_0031501; GO_0005635 5299 YNL159C ASI2 Subunit of the inner nuclear membrane Asi ubiquitin ligase complex Subunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids C IDA; IDA; HDA Asi complex; nuclear inner membrane; endoplasmic reticulum GO_0097658; GO_0005637; GO_0005783 5300 YNL160W YGP1 Cell wall-related secretory glycoprotein Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; YGP1 has a paralog, SPS100, that arose from the whole genome duplication C IDA extracellular region GO_0005576 5301 YNL161W CBK1 Serine/threonine protein kinase of the the RAM signaling network Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; HDA; IDA cellular bud tip; cellular bud neck; cytoplasm; nucleus; incipient cellular bud site; mating projection tip; cytoplasmic stress granule; cellular bud; prospore membrane; cell cortex GO_0005934; GO_0005935; GO_0005737; GO_0005634; GO_0000131; GO_0043332; GO_0010494; GO_0005933; GO_0005628; GO_0005938 5302 YNL162W RPL42A Ribosomal 60S subunit protein L42A Ribosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 5303 YNL162W-A YNL162W-A Putative protein of unknown function Putative protein of unknown function; identified by homology C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5304 YNL163C RIA1 Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes C IDA; HDA cytoplasm; cytosol GO_0005737; GO_0005829 5305 YNL164C IBD2 Component of the BUB2-dependent spindle checkpoint pathway Component of the BUB2-dependent spindle checkpoint pathway; interacts with Bfa1p and functions upstream of Bub2p and Bfa1p 5306 YNL165W YNL165W Putative protein of unknown function Putative protein of unknown function; YNL165W is not an essential gene 5307 YNL166C BNI5 Linker protein responsible for recruitment of myosin to the bud neck Protein involved in organization of septins at the mother-bud neck; may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner C HDA; IDA cellular bud neck; cellular bud neck septin ring GO_0005935; GO_0000144 5308 YNL167C SKO1 Basic leucine zipper transcription factor of the ATF/CREB family Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 5309 YNL168C FMP41 Putative protein of unknown function Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5310 YNL169C PSD1 Phosphatidylserine decarboxylase of the mitochondrial inner membrane Phosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology by affecting lipid mixing in the mitochondrial membrane and by influencing the ratio of long to short forms of Mgm1p; partly exposed to the mitochondrial intermembrane space C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 5311 YNL170W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5312 YNL171C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5313 YNL172W APC1 Largest subunit of the Anaphase-Promoting Complex/Cyclosome Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress C IDA; HDA anaphase-promoting complex; nucleus GO_0005680; GO_0005634 5314 YNL173C MDG1 Plasma membrane protein Plasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication C HDA; HDA; HDA; IDA cytoplasm; nucleus; plasma membrane; membrane raft GO_0005737; GO_0005634; GO_0005886; GO_0045121 5315 YNL174W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NOP13/YNL175C 5316 YNL175C NOP13 Nucleolar protein found in preribosomal complexes Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress C IDA; HDA; HDA preribosome; nucleolus; nucleus GO_0030684; GO_0005730; GO_0005634 5317 YNL176C TDA7 Cell cycle-regulated gene of unknown function Cell cycle-regulated gene of unknown function; promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele; TDA7 has a paralog, YDL211C, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 5318 YNL177C MRPL22 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 5319 YNL178W RPS3 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 C IDA; IDA; HDA preribosome, small subunit precursor; cytosolic small ribosomal subunit; 90S preribosome GO_0030688; GO_0022627; GO_0030686 5320 YNL179C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance 5321 YNL180C RHO5 Non-essential small GTPase of the Rho/Rac family of Ras-like proteins Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity C HDA; IDA; IDA; IDA; HDA cytoplasm; plasma membrane; fungal-type vacuole membrane; endomembrane system; nucleus GO_0005737; GO_0005886; GO_0000329; GO_0012505; GO_0005634 5322 YNL181W PBR1 Putative oxidoreductase Putative oxidoreductase; required for cell viability C HDA; HDA endoplasmic reticulum; nuclear envelope GO_0005783; GO_0005635 5323 YNL182C IPI3 Component of the Rix1 complex and pre-replicative complexes (pre-RCs) Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p C IDA; IDA; HDA; IDA nuclear pre-replicative complex; Rix1 complex; nucleus; nucleoplasm GO_0005656; GO_0097344; GO_0005634; GO_0005654 5324 YNL183C NPR1 Protein kinase Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex; NPR1 has a paralog, PRR2, that arose from the whole genome duplication C IDA; IDA; IDA; HDA plasma membrane; cytoplasm; Golgi apparatus; cytosol GO_0005886; GO_0005737; GO_0005794; GO_0005829 5325 YNL184C YNL184C Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels 5326 YNL185C MRPL19 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 5327 YNL186W UBP10 Ubiquitin-specific protease, deubiquitinates Ub-protein moieties Ubiquitin-specific protease, deubiquitinates Ub-protein moieties; interacts with proteins that function in rRNA production and ribosome biogenesis; stabilizes Rpa190p, the largest subunit of RNAP I, by mediating its deubiquitination; controls PCNA deubiquitylation; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; localized to the nucleolus; human USP36 is a functional analog of Ubp10p C IDA; IDA nucleolus; nucleus GO_0005730; GO_0005634 5328 YNL187W SWT21 Protein involved in mRNA splicing Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats C IDA; IDA nucleus; small nuclear ribonucleoprotein complex GO_0005634; GO_0030532 5329 YNL188W KAR1 Protein involved in karyogamy and spindle pole body duplication Protein involved in karyogamy and spindle pole body duplication; involved in karyogamy during mating; involved in spindle pole body duplication during mitosis; localizes to the half-bridge of the spindle pole body; interacts with Spc72p during karyogamy; also interacts with Cdc31p; essential gene C IDA; HDA half bridge of spindle pole body; endoplasmic reticulum GO_0005825; GO_0005783 5330 YNL189W SRP1 Karyopherin alpha homolog Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation C IDA; IDA; HDA nuclear exosome (RNase complex); cytoplasm; nucleus GO_0000176; GO_0005737; GO_0005634 5331 YNL190W YNL190W Hydrophilin essential in desiccation-rehydration process Hydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site C IDA fungal-type cell wall GO_0009277 5332 YNL191W DUG3 Component of glutamine amidotransferase (GATase II) Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) C IDA; IDA glutathione hydrolase complex; cytoplasm GO_0061672; GO_0005737 5333 YNL192W CHS1 Chitin synthase I Chitin synthase I; requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor C HDA; IDA; IDA cell periphery; chitosome; plasma membrane GO_0071944; GO_0045009; GO_0005886 5334 YNL193W YNL193W Putative protein of unknown function Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis 5335 YNL194C YNL194C Integral membrane protein Integral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication C HDA; HDA; HDA; IDA; HDA cell periphery; plasma membrane; cytoplasm; cell cortex; endoplasmic reticulum GO_0071944; GO_0005886; GO_0005737; GO_0005938; GO_0005783 5336 YNL195C YNL195C Protein of unknown function Protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YNL195C has a paralog, HBT1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 5337 YNL196C SLZ1 Sporulation-specific protein with a leucine zipper motif Sporulation-specific protein with a leucine zipper motif; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation C IPI MIS complex GO_0036396 5338 YNL197C WHI3 RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; regulates genes involved in the cell cycle, sister chromatid cohesion, and stress response; acts as a cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; Tpk1p (PKA) mediated phosphorylation (S568) inhibits Whi3p function, decreasing its interaction with CLN3 mRNA; regulates ploidy C IDA; IDA; IDA cytoplasm; cytoplasmic stress granule; P-body GO_0005737; GO_0010494; GO_0000932 5339 YNL198C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5340 YNL199C GCR2 Transcriptional activator of genes involved in glycolysis Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p C IDA; IDA nucleus; nuclear envelope GO_0005634; GO_0005635 5341 YNL200C NNR1 NADHX epimerase NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 5342 YNL201C PSY2 Subunit of protein phosphatase PP4 complex Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 C IDA; IDA; HDA protein phosphatase 4 complex; condensed nuclear chromosome; nucleus GO_0030289; GO_0000794; GO_0005634 5343 YNL202W SPS19 Peroxisomal 2,4-dienoyl-CoA reductase Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate C IDA peroxisomal matrix GO_0005782 5344 YNL203C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5345 YNL204C SPS18 Protein of unknown function, contains a putative zinc-binding domain Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation; SPS18 has a paralog, GCS1, that arose from the whole genome duplication 5346 YNL205C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5347 YNL206C RTT106 Histone chaperone Histone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1) C HDA nucleus GO_0005634 5348 YNL207W RIO2 Essential serine kinase involved in the processing of 20S pre-rRNA Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p C IDA; IDA; IDA; IDA nucleus; cytoplasmic stress granule; cytoplasm; cytosol GO_0005634; GO_0010494; GO_0005737; GO_0005829 5349 YNL208W YNL208W Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi C HDA; HDA ribosome; mitochondrion GO_0005840; GO_0005739 5350 YNL209W SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA polysome; cytoplasm; plasma membrane; cytoplasmic stress granule GO_0005844; GO_0005737; GO_0005886; GO_0010494 5351 YNL210W MER1 mRNA-binding protein required for meiosis-specific mRNA splicing mRNA-binding protein required for meiosis-specific mRNA splicing; required for chromosome pairing and meiotic recombination; Mer1p regulon embraces four essential meiotic pre-mRNAs: REC107, HFM1, AMA1 and SPO22 C IDA; IDA U1 snRNP; nucleus GO_0005685; GO_0005634 5352 YNL211C MRX7 Protein that associates with mitochondrial ribosome Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene C HDA mitochondrion GO_0005739 5353 YNL212W VID27 Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth C HDA cytoplasm GO_0005737 5354 YNL213C RRG9 Protein of unknown function Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5355 YNL214W PEX17 Membrane peroxin of the peroxisomal importomer complex Membrane peroxin of the peroxisomal importomer complex; complex facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis C IDA; IMP; IDA Pex17p-Pex14p docking complex; peroxisomal membrane; peroxisomal importomer complex GO_1990415; GO_0005778; GO_1990429 5356 YNL215W IES2 Protein that associates with the INO80 chromatin remodeling complex Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress C IPI; HDA Ino80 complex; nucleus GO_0031011; GO_0005634 5357 YNL216W RAP1 Essential DNA-binding transcription regulator that binds many loci Essential DNA-binding transcription regulator that binds many loci; involved in transcription activation and repression, chromatin silencing, and telomere length maintenance; relocalizes to the cytosol in response to hypoxia; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) C IDA; IDA; IMP; IDA; IDA cytosol; shelterin complex; nuclear chromosome, telomeric region; nucleus; nuclear chromosome GO_0005829; GO_0070187; GO_0000784; GO_0005634; GO_0000228 5358 YNL217W PPN2 Zn2+-dependent endopolyphosphatase Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate C HDA; IDA; HDA fungal-type vacuole membrane; vacuolar lumen; fungal-type vacuole GO_0000329; GO_0005775; GO_0000324 5359 YNL218W MGS1 Protein with DNA-dependent ATPase and ssDNA annealing activities Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5360 YNL219C ALG9 Mannosyltransferase, involved in N-linked glycosylation Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation C IMP endoplasmic reticulum GO_0005783 5361 YNL220W ADE12 Adenylosuccinate synthase Adenylosuccinate synthase; catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence C HDA cytoplasm GO_0005737 5362 YNL221C POP1 Subunit of RNase MRP, nuclear RNase P and telomerase complexes Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P C IDA; IPI; IDA nucleolar ribonuclease P complex; ribonuclease MRP complex; telomerase holoenzyme complex GO_0005655; GO_0000172; GO_0005697 5363 YNL222W SSU72 Phosphatase and transcription/RNA-processing factor Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo C IPI; IGI mRNA cleavage and polyadenylation specificity factor complex; nucleus GO_0005847; GO_0005634 5364 YNL223W ATG4 Conserved cysteine protease required for autophagy Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation C IDA cytosol GO_0005829 5365 YNL224C SQS1 Protein that stimulates the ATPase and helicase activities of Prp43p Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress C IDA; HDA; IDA; HDA; HDA; IDA 90S preribosome; cytoplasm; preribosome, small subunit precursor; nucleus; nucleolus; preribosome, large subunit precursor GO_0030686; GO_0005737; GO_0030688; GO_0005634; GO_0005730; GO_0030687 5366 YNL225C CNM67 Component of the spindle pole body outer plaque Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication C IDA; IDA spindle pole body; outer plaque of spindle pole body GO_0005816; GO_0005824 5367 YNL226W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C 5368 YNL227C JJJ1 Co-chaperone that stimulates the ATPase activity of Ssa1p Co-chaperone that stimulates the ATPase activity of Ssa1p; required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis C HDA; HDA; HDA; IDA; HDA; IMP mitochondrion; cytoplasm; cytosol; cytosolic large ribosomal subunit; preribosome, large subunit precursor; nucleolus GO_0005739; GO_0005737; GO_0005829; GO_0022625; GO_0030687; GO_0005730 5369 YNL228W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 5370 YNL229C URE2 Nitrogen catabolite repression transcriptional regulator Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion C IDA cytoplasm GO_0005737 5371 YNL230C ELA1 Elongin A Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex C HDA; IPI; HDA; IMP nucleus; elongin complex; cytoplasm; Cul3-RING ubiquitin ligase complex GO_0005634; GO_0070449; GO_0005737; GO_0031463 5372 YNL231C PDR16 Phosphatidylinositol transfer protein (PITP) Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress C IDA; HDA; HDA; IDA; HDA; IDA lipid particle; plasma membrane; cytosol; nucleus-vacuole junction; cytoplasm; cell periphery GO_0005811; GO_0005886; GO_0005829; GO_0071561; GO_0005737; GO_0071944 5373 YNL232W CSL4 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) C IDA; IDA; IDA; IDA cytoplasm; cytoplasmic exosome (RNase complex); nuclear exosome (RNase complex); nucleus GO_0005737; GO_0000177; GO_0000176; GO_0005634 5374 YNL233W BNI4 Targeting subunit for Glc7p protein phosphatase Targeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p C IDA; IDA; IDA; IDA septin ring; incipient cellular bud site; cellular bud neck; cellular bud neck septin collar GO_0005940; GO_0000131; GO_0005935; GO_0032174 5375 YNL234W YNL234W Protein of unknown function with similarity to globins Protein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1 C HDA; HDA fungal-type vacuole membrane; cytoplasm GO_0000329; GO_0005737 5376 YNL235C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex 5377 YNL236W SIN4 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription C IDA; IDA mediator complex; core mediator complex GO_0016592; GO_0070847 5378 YNL237W YTP1 Probable type-III integral membrane protein of unknown function Probable type-III integral membrane protein of unknown function; has regions of similarity to mitochondrial electron transport proteins 5379 YNL238W KEX2 Kexin, a subtilisin-like protease (proprotein convertase) Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway C HDA; IDA fungal-type vacuole; trans-Golgi network GO_0000324; GO_0005802 5380 YNL239W LAP3 Cysteine aminopeptidase with homocysteine-thiolactonase activity Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 5381 YNL240C NAR1 Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery; required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; deficiency results in shortened lifespan and sensitivity to paraquat; homologous to human Narf C IDA; IDA membrane; cytosol GO_0016020; GO_0005829 5382 YNL241C ZWF1 Glucose-6-phosphate dehydrogenase (G6PD) Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 5383 YNL242W ATG2 Peripheral membrane protein required for autophagic vesicle formation Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress C IDA; HDA; IDA; IDA; HDA pre-autophagosomal structure; cytoplasm; NA; extrinsic component of membrane; cytosol GO_0000407; GO_0005737; GO_0061908; GO_0019898; GO_0005829 5384 YNL243W SLA2 Adaptor protein that links actin to clathrin and endocytosis Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo C HDA; HDA; IDA; IDA cellular bud neck; mating projection tip; actin cortical patch; incipient cellular bud site GO_0005935; GO_0043332; GO_0030479; GO_0000131 5385 YNL244C SUI1 Translation initiation factor eIF1 Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase C IDA; IDA eukaryotic 43S preinitiation complex; multi-eIF complex GO_0016282; GO_0043614 5386 YNL245C CWC25 Splicing factor required for the first step of pre-mRNA splicing Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p C IDA U2-type spliceosomal complex GO_0005684 5387 YNL246W VPS75 NAP family histone chaperone NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia C IDA; HDA; IDA chromatin; nucleus; cytosol GO_0000785; GO_0005634; GO_0005829 5388 YNL247W YNL247W Cysteinyl-tRNA synthetase Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments C HDA; HDA cytoplasm; ribosome GO_0005737; GO_0005840 5389 YNL248C RPA49 RNA polymerase I subunit A49 RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p C IDA; IDA DNA-directed RNA polymerase I complex; nucleolus GO_0005736; GO_0005730 5390 YNL249C MPA43 Protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5391 YNL250W RAD50 Subunit of MRX complex with Mre11p and Xrs2p Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress C HDA; HDA; IPI nucleus; mitochondrion; Mre11 complex GO_0005634; GO_0005739; GO_0030870 5392 YNL251C NRD1 RNA-binding subunit of Nrd1 complex RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size C IDA Nrd1 complex GO_0035649 5393 YNL252C MRPL17 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 5394 YNL253W TEX1 Protein involved in mRNA export Protein involved in mRNA export; component of the transcription export (TREX) complex C IDA transcription export complex GO_0000346 5395 YNL254C RTC4 Protein of unknown function Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5396 YNL255C GIS2 Translational activator for mRNAs with internal ribosome entry sites Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 C IPI; HDA; IDA; IDA polysomal ribosome; cytoplasm; P-body; cytoplasmic stress granule GO_0042788; GO_0005737; GO_0000932; GO_0010494 5397 YNL256W FOL1 Multifunctional enzyme of the folic acid biosynthesis pathway Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities C IDA; IDA; HDA mitochondrial envelope; cytoplasm; mitochondrion GO_0005740; GO_0005737; GO_0005739 5398 YNL257C SIP3 Putative sterol transfer protein Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication C HDA; HDA; IDA; HDA plasma membrane; cytoplasm; cortical endoplasmic reticulum; endoplasmic reticulum GO_0005886; GO_0005737; GO_0032541; GO_0005783 5399 YNL258C DSL1 Peripheral membrane protein needed for Golgi-to-ER retrograde traffic Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene C HDA; IDA; IDA; IDA; IDA cytosol; Dsl1/NZR complex; nucleus; endoplasmic reticulum; peroxisome GO_0005829; GO_0070939; GO_0005634; GO_0005783; GO_0005777 5400 YNL259C ATX1 Cytosolic copper metallochaperone Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake C IDA cytosol GO_0005829 5401 YNL260C LTO1 Essential protein that forms a complex with Rli1p and Yae1p Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5402 YNL261W ORC5 Subunit of the origin recognition complex (ORC) Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing C IMP; IDA; IDA nuclear origin of replication recognition complex; nuclear pre-replicative complex; DNA replication preinitiation complex GO_0005664; GO_0005656; GO_0031261 5403 YNL262W POL2 Catalytic subunit of DNA polymerase (II) epsilon Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p C IDA; IDA replication fork; epsilon DNA polymerase complex GO_0005657; GO_0008622 5404 YNL263C YIF1 Integral membrane protein Integral membrane protein; required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 C IDA; IDA; IPI ER to Golgi transport vesicle; integral component of Golgi membrane; endoplasmic reticulum membrane GO_0030134; GO_0030173; GO_0005789 5405 YNL264C PDR17 Phosphatidylinositol transfer protein (PITP) Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition C IDA; IDA; HDA; HDA endosome; cell periphery; cytoplasm; cytosol GO_0005768; GO_0071944; GO_0005737; GO_0005829 5406 YNL265C IST1 Protein with positive role in the multivesicular body sorting pathway Protein with positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p C IDA; HDA cytoplasm; endosome GO_0005737; GO_0005768 5407 YNL266W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C 5408 YNL267W PIK1 Phosphatidylinositol 4-kinase Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p C IDA; IDA nucleus; trans-Golgi network GO_0005634; GO_0005802 5409 YNL268W LYP1 Lysine permease Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids C HDA mitochondrion GO_0005739 5410 YNL269W BSC4 Protein of unknown function Protein of unknown function; protein-coding gene that evolved de novo via a series of point mutations in noncoding sequence; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; may be involved in DNA repair pathway during stationary phase and contribute to robustness of cells when shifted to a nutrient-poor environment 5411 YNL270C ALP1 Arginine transporter Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5412 YNL271C BNI1 Formin Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 C IDA; HDA; IDA; IDA; IDA; IDA; IDA; IDA; HDA polarisome; prospore membrane; incipient cellular bud site; actin filament; cellular bud neck; cell division site; cellular bud tip; mating projection tip; plasma membrane GO_0000133; GO_0005628; GO_0000131; GO_0005884; GO_0005935; GO_0032153; GO_0005934; GO_0043332; GO_0005886 5413 YNL272C SEC2 Guanyl-nucleotide exchange factor for the small G-protein Sec4p Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles C IDA; IDA; IDA; HDA; IDA cellular bud neck; cytosol; cellular bud tip; mating projection tip; transport vesicle GO_0005935; GO_0005829; GO_0005934; GO_0043332; GO_0030133 5414 YNL273W TOF1 Subunit of a replication-pausing checkpoint complex Subunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IPI; IDA nucleus; cytosol; nuclear replication fork; nuclear chromosome; replication fork protection complex GO_0005634; GO_0005829; GO_0043596; GO_0000228; GO_0031298 5415 YNL274C GOR1 Glyoxylate reductase Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress C HDA; HDA; HDA mitochondrion; cytoplasm; nucleus GO_0005739; GO_0005737; GO_0005634 5416 YNL275W BOR1 Boron efflux transporter of the plasma membrane Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 C IDA; IDA; HDA fungal-type vacuole; plasma membrane; cell periphery GO_0000324; GO_0005886; GO_0071944 5417 YNL276C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W 5418 YNL277W MET2 L-homoserine-O-acetyltransferase L-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway C HDA cytoplasm GO_0005737 5419 YNL277W-A YNL277W-A Putative protein of unknown function Putative protein of unknown function 5420 YNL278W CAF120 Part of the CCR4-NOT transcriptional regulatory complex Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication 5421 YNL279W PRM1 Pheromone-regulated multispanning membrane protein Pheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p C IDA; HDA mating projection tip; fungal-type vacuole GO_0043332; GO_0000324 5422 YNL280C ERG24 C-14 sterol reductase C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions C HDA endoplasmic reticulum GO_0005783 5423 YNL281W HCH1 Heat shock protein regulator Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5424 YNL282W POP3 Subunit of both RNase MRP and nuclear RNase P Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia C IPI; IPI; IDA; IDA nucleolar ribonuclease P complex; ribonuclease MRP complex; cytosol; nucleus GO_0005655; GO_0000172; GO_0005829; GO_0005634 5425 YNL283C WSC2 Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication C HDA; IDA; IDA; HDA; HDA cytoplasm; cellular bud; site of polarized growth; fungal-type vacuole; mating projection tip GO_0005737; GO_0005933; GO_0030427; GO_0000324; GO_0043332 5426 YNL284C MRPL10 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 5427 YNL284C-A YNL284C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 5428 YNL284C-B YNL284C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 5429 YNL285W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5430 YNL286W CUS2 Putative checkpoint factor in transcription Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) C IPI U2 snRNP GO_0005686 5431 YNL287W SEC21 Gamma subunit of coatomer Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo C IDA; IDA endosome; COPI vesicle coat GO_0005768; GO_0030126 5432 YNL288W CAF40 Component of the CCR4-NOT transcriptional complex Component of the CCR4-NOT transcriptional complex; evolutionarily conserved; involved in controlling mRNA initiation, elongation, and degradation; binds Cdc39p C IPI CCR4-NOT core complex GO_0030015 5433 YNL289W PCL1 Cyclin, interacts with cyclin-dependent kinase Pho85p Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth C IDA; IDA; IPI nucleus; incipient cellular bud site; cyclin-dependent protein kinase holoenzyme complex GO_0005634; GO_0000131; GO_0000307 5434 YNL290W RFC3 Subunit of heteropentameric Replication factor C (RF-C) Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA; IDA; IDA; IPI Elg1 RFC-like complex; cytosol; nucleus; Rad17 RFC-like complex; DNA replication factor C complex; Ctf18 RFC-like complex GO_0031391; GO_0005829; GO_0005634; GO_0031389; GO_0005663; GO_0031390 5435 YNL291C MID1 N-glycosylated integral membrane protein of the ER and plasma membrane N-glycosylated integral membrane protein of the ER and plasma membrane; functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer C IDA; IDA plasma membrane; endoplasmic reticulum GO_0005886; GO_0005783 5436 YNL292W PUS4 Pseudouridine synthase Pseudouridine synthase; catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; also responsible for pseudouracil modification of some mRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) C IMP; IMP mitochondrion; nucleus GO_0005739; GO_0005634 5437 YNL293W MSB3 Rab GTPase-activating protein Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; required for proper actin organization; also localizes to plasma membrane and sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to the TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication C HDA; HDA; IDA; HDA; IDA; IDA; HDA; IDA; IDA; IDA cellular bud tip; cytoplasm; incipient cellular bud site; cellular bud neck; fungal-type vacuole membrane; cytosol; cell periphery; late endosome; plasma membrane; polarisome GO_0005934; GO_0005737; GO_0000131; GO_0005935; GO_0000329; GO_0005829; GO_0071944; GO_0005770; GO_0005886; GO_0000133 5438 YNL294C RIM21 pH sensor molecule, component of the RIM101 pathway pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 5439 YNL295W MRX6 Protein that associates with mitochondrial ribosome Putative protein of unknown function 5440 YNL296W Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog 5441 YNL297C MON2 Protein with a role in endocytosis and vacuole integrity Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins C IDA; IDA; IDA; IDA trans-Golgi network; extrinsic component of membrane; endosome; early endosome membrane GO_0005802; GO_0019898; GO_0005768; GO_0031901 5442 YNL298W CLA4 Cdc42p-activated signal transducing kinase Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication C IDA; IMP; IDA cellular bud; nucleus; fungal-type vacuole GO_0005933; GO_0005634; GO_0000324 5443 YNL299W TRF5 Non-canonical poly(A) polymerase Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p C HDA; IDA nucleolus; TRAMP complex GO_0005730; GO_0031499 5444 YNL300W TOS6 Glycosylphosphatidylinositol-dependent cell wall protein Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid C HDA; IDA cytosol; fungal-type cell wall GO_0005829; GO_0009277 5445 YNL301C RPL18B Ribosomal 60S subunit protein L18B Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 5446 YNL302C RPS19B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 5447 YNL303W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5448 YNL304W YPT11 Rab GTPase Rab GTPase; Myo2p-binding protein implicated in mother-to-bud transport of cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during cell division; function is regulated at multiple levels; abundance of active Ypt11p forms is controlled by phosphorylation status and degradation; normally a low-abundance protein whose ER localization is only detected when protein is highly over expressed C IDA; IDA; IDA; IDA; IDA incipient cellular bud site; cellular bud neck; mitochondrion; endoplasmic reticulum; cellular bud tip GO_0000131; GO_0005935; GO_0005739; GO_0005783; GO_0005934 5449 YNL305C BXI1 Protein involved in apoptosis Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p C HDA; IDA; IDA fungal-type vacuole; endoplasmic reticulum; mitochondrion GO_0000324; GO_0005783; GO_0005739 5450 YNL306W MRPS18 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 5451 YNL307C MCK1 Dual-specificity ser/thr and tyrosine protein kinase Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication 5452 YNL308C KRI1 Essential nucleolar protein required for 40S ribosome biogenesis Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p C IDA; IDA 90S preribosome; nucleolus GO_0030686; GO_0005730 5453 YNL309W STB1 Protein with role in regulation of MBF-specific transcription at Start Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication C IDA; HDA; IDA; IDA; HDA SBF transcription complex; cytoplasm; Sin3-type complex; MBF transcription complex; nucleus GO_0033309; GO_0005737; GO_0070822; GO_0030907; GO_0005634 5454 YNL310C ZIM17 Protein co-chaperone with a zinc finger motif Protein co-chaperone with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix; required for the maintenance of Ssc1p solubility and assists in the functional interaction of Ssc1p with substrate proteins C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 5455 YNL311C SKP2 F-box protein of unknown function F-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments C IDA; HDA SCF ubiquitin ligase complex; ribosome GO_0019005; GO_0005840 5456 YNL312W RFA2 Subunit of heterotrimeric Replication Protein A (RPA) Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s C IMP; IDA; IDA chromosome, telomeric region; condensed nuclear chromosome; DNA replication factor A complex GO_0000781; GO_0000794; GO_0005662 5457 YNL313C EMW1 Essential conserved protein with a role in cell wall integrity Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5458 YNL314W DAL82 Positive regulator of allophanate inducible genes Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain C IDA nucleus GO_0005634 5459 YNL315C ATP11 Molecular chaperone Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo C IDA mitochondrion GO_0005739 5460 YNL316C PHA2 Prephenate dehydratase Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway C HDA cytoplasm GO_0005737 5461 YNL317W PFS2 Integral subunit of the pre-mRNA CPF complex Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex C IDA mRNA cleavage and polyadenylation specificity factor complex GO_0005847 5462 YNL318C HXT14 Protein with similarity to hexose transporter family members Protein with similarity to hexose transporter family members; expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA; HDA cell periphery; fungal-type vacuole; mitochondrion GO_0071944; GO_0000324; GO_0005739 5463 YNL319W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HXT14 5464 YNL320W YNL320W Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA fungal-type vacuole; mitochondrion GO_0000324; GO_0005739 5465 YNL321W VNX1 Calcium/H+ antiporter localized to the endoplasmic reticulum membrane Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate C HDA; IDA; IDA; IDA fungal-type vacuole membrane; vacuole-mitochondrion membrane contact site; fungal-type vacuole; endoplasmic reticulum membrane GO_0000329; GO_1990816; GO_0000324; GO_0005789 5466 YNL322C KRE1 Cell wall glycoprotein involved in beta-glucan assembly Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor C IDA; HDA fungal-type cell wall; fungal-type vacuole GO_0009277; GO_0000324 5467 YNL323W LEM3 Membrane protein of the plasma membrane and ER Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p C IPI; IDA; IDA; HDA Lem3p-Dnf1p complex; endoplasmic reticulum; plasma membrane; cytoplasm GO_1990531; GO_0005783; GO_0005886; GO_0005737 5468 YNL324W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5469 YNL325C FIG4 Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder C IDA; IDA; IDA; IPI extrinsic component of membrane; nuclear periphery; fungal-type vacuole membrane; PAS complex GO_0019898; GO_0034399; GO_0000329; GO_0070772 5470 YNL326C PFA3 Palmitoyltransferase for Vac8p Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions C IDA; HDA fungal-type vacuole membrane; fungal-type vacuole GO_0000329; GO_0000324 5471 YNL327W EGT2 Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase; required for proper cell separation after cytokinesis; expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner C HDA; IDA; IDA; IDA fungal-type vacuole; cell septum; cellular bud; fungal-type cell wall GO_0000324; GO_0030428; GO_0005933; GO_0009277 5472 YNL328C MDJ2 Constituent of the mitochondrial import motor Constituent of the mitochondrial import motor; associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain C IPI; IDA presequence translocase-associated import motor; mitochondrial inner membrane GO_0001405; GO_0005743 5473 YNL329C PEX6 AAA-peroxin AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol C IDA; IDA peroxisome; cytosol GO_0005777; GO_0005829 5474 YNL330C RPD3 Histone deacetylase, component of both the Rpd3S and Rpd3L complexes Histone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p C IDA; HDA; HDA; IDA; HDA; IDA; IDA Rpd3S complex; Snt2C complex; Rpd3L complex; histone deacetylase complex; Rpd3L-Expanded complex; nuclear periphery; Sin3-type complex GO_0032221; GO_0070211; GO_0033698; GO_0000118; GO_0070210; GO_0034399; GO_0070822 5475 YNL331C AAD14 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 5476 YNL332W THI12 Protein involved in synthesis of the thiamine precursor HMP Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP 5477 YNL333W SNZ2 Member of a stationary phase-induced gene family Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p 5478 YNL334C SNO2 Protein of unknown function Protein of unknown function; nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin 5479 YNL335W DDI3 Cyanamide hydratase that detoxifies cyanamide Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants 5480 YNL336W COS1 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA; IDA; HDA fungal-type vacuole; endosome; fungal-type vacuole membrane GO_0000324; GO_0005768; GO_0000329 5481 YNL337W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5482 YNL338W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is a telomeric X element core sequence on the left arm of chromosome XIV 5483 YNL339C YRF1-6 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 5484 YNL339W-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5485 YNL339W-B Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5486 YNR001C CIT1 Citrate synthase Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 5487 YNR001W-A Dubious open reading frame unlikely to encode a functional protein; identified by homology 5488 YNR002C ATO2 Putative transmembrane protein involved in export of ammonia Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication C HDA; IDA; HDA mitochondrion; plasma membrane; fungal-type vacuole GO_0005739; GO_0005886; GO_0000324 5489 YNR003C RPC34 RNA polymerase III subunit C34 RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex C IPI; IDA; HDA; HDA mitochondrion; DNA-directed RNA polymerase III complex; nucleus; cytoplasm GO_0005739; GO_0005666; GO_0005634; GO_0005737 5490 YNR003W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene RPC34/YNR003C 5491 YNR004W SWM2 Protein with a role in snRNA and snoRNA cap trimethylation Protein with a role in snRNA and snoRNA cap trimethylation; interacts with Tgs1p and shows similar phenotypes; required for trimethylation of the caps of spliceosomal snRNAs and the U3 snoRNA, and for efficient 3' end processing of U3 snoRNA; may act as a specificity factor for Tgs1p C HDA nucleolus GO_0005730 5492 YNR005C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5493 YNR006W VPS27 Endosomal protein that forms a complex with Hse1p Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) C IPI; IDA ESCRT-0 complex; endosome GO_0033565; GO_0005768 5494 YNR007C ATG3 E2-like enzyme E2-like enzyme; involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p C IDA; IMP; IDA; HDA pre-autophagosomal structure; NA; cytosol; cytoplasm GO_0000407; GO_0061908; GO_0005829; GO_0005737 5495 YNR008W LRO1 Acyltransferase that catalyzes diacylglycerol esterification Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase C IDA; IDA; HDA lipid particle; perinuclear endoplasmic reticulum; endoplasmic reticulum GO_0005811; GO_0097038; GO_0005783 5496 YNR009W NRM1 Transcriptional co-repressor of MBF-regulated gene expression Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase C HDA; IDA; HDA nucleus; MBF transcription complex; cytoplasm GO_0005634; GO_0030907; GO_0005737 5497 YNR010W CSE2 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA nucleus; core mediator complex; cytosol GO_0005634; GO_0070847; GO_0005829 5498 YNR011C PRP2 RNA-dependent DExD/H-box ATPase RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16 C IMP U2-type catalytic step 1 spliceosome GO_0071006 5499 YNR012W URK1 Uridine/cytidine kinase Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5500 YNR013C PHO91 Low-affinity vacuolar phosphate transporter Low-affinity vacuolar phosphate transporter; exports phosphate from the vacuolar lumen to the cytosol; regulates phosphate and polyphosphate metabolism; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth C IDA; IDA fungal-type vacuole membrane; vacuole-mitochondrion membrane contact site GO_0000329; GO_1990816 5501 YNR014W YNR014W Putative protein of unknown function Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 5502 YNR015W SMM1 Dihydrouridine synthase Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5503 YNR016C ACC1 Acetyl-CoA carboxylase, biotin containing enzyme Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; ACC1 has a paralog, HFA1, that arose from the whole genome duplication C HDA; IDA; HDA cytosol; endoplasmic reticulum membrane; mitochondrion GO_0005829; GO_0005789; GO_0005739 5504 YNR017W TIM23 Essential component of the TIM23 complex Essential component of the TIM23 complex; involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel; TIM23 complex is short for the translocase of the inner mitochondrial membrane C HDA; IPI mitochondrion; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0005744 5505 YNR018W RCF2 Cytochrome c oxidase subunit Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast C HDA; IDA; IDA mitochondrion; mitochondrial respiratory chain supercomplex; integral component of mitochondrial inner membrane GO_0005739; GO_0097249; GO_0031305 5506 YNR019W ARE2 Acyl-CoA:sterol acyltransferase Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5507 YNR020C ATP23 Putative metalloprotease of the mitochondrial inner membrane Putative metalloprotease of the mitochondrial inner membrane; required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p C IDA; IDA mitochondrial intermembrane space; extrinsic component of mitochondrial inner membrane GO_0005758; GO_0031314 5508 YNR021W YNR021W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene C HDA endoplasmic reticulum GO_0005783 5509 YNR022C MRPL50 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 5510 YNR023W SNF12 73 kDa subunit of the SWI/SNF chromatin remodeling complex 73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; SWI/SNF complex; cytosol GO_0005634; GO_0016514; GO_0005829 5511 YNR024W MPP6 Nuclear exosome-associated RNA binding protein Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; HDA cytosol; nucleus; TRAMP complex; nuclear exosome (RNase complex); ribosome GO_0005829; GO_0005634; GO_0031499; GO_0000176; GO_0005840 5512 YNR025C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase 5513 YNR026C SEC12 Guanine nucleotide exchange factor (GEF) Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication C IDA; IDA endoplasmic reticulum; Golgi apparatus GO_0005783; GO_0005794 5514 YNR027W BUD17 Putative pyridoxal kinase Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5515 YNR028W CPR8 Peptidyl-prolyl cis-trans isomerase (cyclophilin) Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; CPR8 has a paralog, CPR4, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 5516 YNR029C YNR029C Putative protein of unknown function Putative protein of unknown function; deletion confers reduced fitness in saline C HDA cytoplasm GO_0005737 5517 YNR030W ALG12 Alpha-1,6-mannosyltransferase localized to the ER Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation C HDA endoplasmic reticulum GO_0005783 5518 YNR031C SSK2 MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA cell cortex; cellular bud neck; incipient cellular bud site; cytosol; cellular bud tip GO_0005938; GO_0005935; GO_0000131; GO_0005829; GO_0005934 5519 YNR032C-A HUB1 Ubiquitin-like protein modifier Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear C IPI mating projection GO_0005937 5520 YNR032W PPG1 Putative serine/threonine protein phosphatase Putative serine/threonine protein phosphatase; putative phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5521 YNR033W ABZ1 Para-aminobenzoate (PABA) synthase Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 5522 YNR034W SOL1 Protein with a possible role in tRNA export Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5523 YNR034W-A EGO4 Protein of unknown function Putative protein of unknown function; expression is regulated by Msn2p/Msn4p; YNR034W-A has a paralog, YCR075W-A, that arose from the whole genome duplication C HDA; IDA cytoplasm; cytosol GO_0005737; GO_0005829 5524 YNR035C ARC35 Subunit of the ARP2/3 complex Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin C IDA; IPI Arp2/3 protein complex; cytosol GO_0005885; GO_0005829 5525 YNR036C MRPS12 Mitochondrial protein Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins C HDA; HDA ribosome; mitochondrion GO_0005840; GO_0005739 5526 YNR037C RSM19 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S19 ribosomal protein C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 5527 YNR038W DBP6 Essential protein involved in ribosome biogenesis Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family C IDA; IDA nucleolus; preribosome, large subunit precursor GO_0005730; GO_0030687 5528 YNR039C ZRG17 Endoplasmic reticulum zinc transporter Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency C HDA endoplasmic reticulum GO_0005783 5529 YNR040W DPI29 Putative protein of unknown function Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5530 YNR041C COQ2 Para hydroxybenzoate polyprenyl transferase Para hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, is able to complement a yeast coq2 null mutant C HDA; IDA mitochondrion; integral component of mitochondrial membrane GO_0005739; GO_0032592 5531 YNR042W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 5532 YNR043W MVD1 Mevalonate pyrophosphate decarboxylase Mevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer C HDA cytoplasm GO_0005737 5533 YNR044W AGA1 Anchorage subunit of a-agglutinin of a-cells Anchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication C IDA; HDA fungal-type cell wall; cell periphery GO_0009277; GO_0071944 5534 YNR045W PET494 Mitochondrial translational activator specific for the COX3 mRNA Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 5535 YNR046W TRM112 Protein involved in methylation of tRNA, rRNA, and translation factors Protein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress C HDA; IPI; HDA; HDA; IPI nucleus; tRNA (m2G10) methyltransferase complex; cytoplasm; nucleolus; eRF1 methyltransferase complex GO_0005634; GO_0043528; GO_0005737; GO_0005730; GO_0035657 5536 YNR047W FPK1 Ser/Thr protein kinase Ser/Thr protein kinase; regulates the putative phospholipid translocases Lem3p-Dnf1p/Dnf2p; phosphorylates and inhibits upstream inhibitory kinase, Ypk1p; localizes to the cytoplasm, early endosome/TGN compartments, and plasma membrane; FPK1 has a paralog, KIN82, that arose from the whole genome duplication C IDA; IDA; IDA mating projection tip; cytoplasm; plasma membrane GO_0043332; GO_0005737; GO_0005886 5537 YNR048W YNR048W Potential noncatalytic subunit for phospholipid translocase Dnf3p Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication C IPI; HDA trans-Golgi network; endoplasmic reticulum GO_0005802; GO_0005783 5538 YNR049C MSO1 Lipid-interacting protein in SNARE complex assembly machinery Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress C HDA; HDA; HDA; HDA; IDA; IDA; HDA; IDA; HDA cellular bud neck; cytoplasm; nucleus; cellular bud tip; SNARE complex; plasma membrane; prospore membrane; cellular bud membrane; cytosol GO_0005935; GO_0005737; GO_0005634; GO_0005934; GO_0031201; GO_0005886; GO_0005628; GO_0033101; GO_0005829 5539 YNR050C LYS9 Saccharopine dehydrogenase (NADP+, L-glutamate-forming) Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 C HDA; HDA cytoplasm; cell periphery GO_0005737; GO_0071944 5540 YNR051C BRE5 Ubiquitin protease cofactor Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A C IDA; HDA; IPI; HDA P-body; cytosol; Ubp3-Bre5 deubiquitination complex; cytoplasm GO_0000932; GO_0005829; GO_1990861; GO_0005737 5541 YNR052C POP2 RNase of the DEDD superfamily RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation C IDA; IDA; IDA; HDA CCR4-NOT core complex; cytoplasm; P-body; mating projection tip GO_0030015; GO_0005737; GO_0000932; GO_0043332 5542 YNR053C NOG2 Putative GTPase Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation C IDA; IDA; HDA; IDA nucleoplasm; preribosome, large subunit precursor; nucleus; nucleolus GO_0005654; GO_0030687; GO_0005634; GO_0005730 5543 YNR054C ESF2 Essential nucleolar protein involved in pre-18S rRNA processing Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome C IDA; IDA; IDA nucleolus; 90S preribosome; cytoplasm GO_0005730; GO_0030686; GO_0005737 5544 YNR055C HOL1 Putative transporter in the major facilitator superfamily Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake C HDA; HDA; HDA mitochondrion; cell periphery; fungal-type vacuole GO_0005739; GO_0071944; GO_0000324 5545 YNR056C BIO5 Putative transmembrane protein involved in the biotin biosynthesis Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis C HDA endoplasmic reticulum GO_0005783 5546 YNR057C BIO4 Dethiobiotin synthetase Dethiobiotin synthetase; catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria; expression appears to be repressed at low iron levels 5547 YNR058W BIO3 7,8-diamino-pelargonic acid aminotransferase (DAPA) 7,8-diamino-pelargonic acid aminotransferase (DAPA); catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria 5548 YNR059W MNT4 Putative alpha-1,3-mannosyltransferase Putative alpha-1,3-mannosyltransferase; not required for protein O-glycosylation 5549 YNR060W FRE4 Ferric reductase Ferric reductase; reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels C IMP plasma membrane GO_0005886 5550 YNR061C YNR061C Protein of unknown function Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress C HDA; HDA; HDA fungal-type vacuole; fungal-type vacuole membrane; cytoplasm GO_0000324; GO_0000329; GO_0005737 5551 YNR062C YNR062C Putative membrane protein of unknown function Putative membrane protein of unknown function C HDA endoplasmic reticulum GO_0005783 5552 YNR063W YNR063W Putative zinc-cluster protein of unknown function Putative zinc-cluster protein of unknown function 5553 YNR064C YNR064C Epoxide hydrolase Epoxide hydrolase; member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides 5554 YNR065C YNR065C Protein of unknown function Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene C HDA endoplasmic reticulum GO_0005783 5555 YNR066C YNR066C Putative membrane-localized protein of unknown function Putative membrane-localized protein of unknown function C HDA; IDA fungal-type vacuole; membrane GO_0000324; GO_0016020 5556 YNR067C DSE4 Daughter cell-specific secreted protein with similarity to glucanases Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother C HDA; IDA; IDA cellular bud neck; cell septum; fungal-type cell wall GO_0005935; GO_0030428; GO_0009277 5557 YNR068C YNR068C Putative protein of unknown function Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form 5558 YNR069C BSC5 Protein of unknown function Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro 5559 YNR070W PDR18 Putative transporter of the ATP-binding cassette (ABC) family Putative transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA cytosol; mitochondrion GO_0005829; GO_0005739 5560 YNR071C YNR071C Putative aldose 1-epimerase Putative aldose 1-epimerase 5561 YNR072W HXT17 Putative transmembrane polyol transporter Hexose transporter; up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose; HXT17 has a paralog, HXT13, that arose from a segmental duplication C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 5562 YNR073C MAN2 Mannitol dehydrogenase Putative mannitol dehydrogenase; YNR073C has a paralog, DSF1, that arose from a segmental duplication 5563 YNR074C AIF1 Mitochondrial cell death effector Mitochondrial cell death effector; translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase C IDA; IDA mitochondrion; nucleus GO_0005739; GO_0005634 5564 YNR075C-A YNR075C-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5565 YNR075W COS10 Endosomal protein involved in turnover of plasma membrane proteins Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins C HDA; HDA; HDA endoplasmic reticulum; nuclear envelope; cytoplasm GO_0005783; GO_0005635; GO_0005737 5566 YNR076W PAU6 Member of the seripauperin multigene family Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme; identical to Pau18p C HDA fungal-type vacuole GO_0000324 5567 YNR077C YNR077C Protein of unknown function, abundance changes with carbon source Protein of unknown function, abundance changes with carbon source 5568 YOL001W PHO80 Cyclin Cyclin; interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling C IPI; IDA; IDA cyclin-dependent protein kinase holoenzyme complex; nucleus; Pho85-Pho80 CDK-cyclin complex GO_0000307; GO_0005634; GO_1990860 5569 YOL002C IZH2 Plasma membrane receptor for plant antifungal osmotin Plasma membrane receptor for plant antifungal protein, osmotin; involved in zinc ion homeostasis, apoptosis; negatively regulates ZRT1 and other functionally divergent genes through CCCTC promoter motif (IzRE); modulates FET3 activity in iron-independent manner; affects gene expression by influencing balance of competition between Msn2p/Msn4p and Nrg1p/Nrg2p for binding to the IzRE; transcription regulated by Zap1p, zinc, fatty acid levels; similar to mammalian adiponectins C HDA; IDA fungal-type vacuole; plasma membrane GO_0000324; GO_0005886 5570 YOL003C PFA4 Palmitoyltransferase with autoacylation activity Palmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases C HDA endoplasmic reticulum GO_0005783 5571 YOL004W SIN3 Component of both the Rpd3S and Rpd3L histone deacetylase complexes Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity C IDA; IDA; HDA; IDA Sin3-type complex; Rpd3S complex; Rpd3L-Expanded complex; Rpd3L complex GO_0070822; GO_0032221; GO_0070210; GO_0033698 5572 YOL005C RPB11 RNA polymerase II subunit B12.5 RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit C IDA DNA-directed RNA polymerase II, core complex GO_0005665 5573 YOL006C TOP1 Topoisomerase I Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks C IPI; IDA; IDA nucleolus; replication fork protection complex; nucleus GO_0005730; GO_0031298; GO_0005634 5574 YOL007C CSI2 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene C HDA; IDA fungal-type vacuole; cellular bud neck GO_0000324; GO_0005935 5575 YOL008W COQ10 Coenzyme Q (ubiquinone) binding protein Coenzyme Q (ubiquinone) binding protein; functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5576 YOL009C MDM12 Mitochondrial outer membrane protein, ERMES complex subunit Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase C HDA; IPI; HDA; HDA; HDA; IDA mitochondrion; ERMES complex; cytoplasm; nucleus; plasma membrane; mitochondrial outer membrane GO_0005739; GO_0032865; GO_0005737; GO_0005634; GO_0005886; GO_0005741 5577 YOL010W RCL1 Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected C IDA; HDA nucleolus; 90S preribosome GO_0005730; GO_0030686 5578 YOL011W PLB3 Phospholipase B (lysophospholipase) involved in lipid metabolism Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication C HDA; IDA fungal-type vacuole; extracellular region GO_0000324; GO_0005576 5579 YOL012C HTZ1 Histone variant H2AZ Histone variant H2AZ; exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin; Htz1p-containing nucleosomes facilitate RNA Pol II passage by affecting correct assembly and modification status of RNA Pol II elongation complexes and by favoring efficient nucleosome remodeling C IPI; HDA nuclear chromatin; nucleus GO_0000790; GO_0005634 5580 YOL013C HRD1 Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) Ubiquitin-protein ligase; functions in ER retention of misfolded proteins; required for ER-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon C IDA; IDA; IDA Hrd1p ubiquitin ligase ERAD-L complex; Hrd1p ubiquitin ligase ERAD-M complex; endoplasmic reticulum membrane GO_0000839; GO_0000838; GO_0005789 5581 YOL013W-A YOL013W-A Putative protein of unknown function Putative protein of unknown function; identified by SAGE 5582 YOL013W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element 5583 YOL014W YOL014W Putative protein of unknown function Putative protein of unknown function 5584 YOL015W IRC10 Protein of unknown function Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci C HDA prospore membrane leading edge GO_0070056 5585 YOL016C CMK2 Calmodulin-dependent protein kinase Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 5586 YOL017W ESC8 Protein involved in telomeric and mating-type locus silencing Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication C IPI nucleus GO_0005634 5587 YOL018C TLG2 Syntaxin-like t-SNARE Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) C IPI; IDA; IDA SNARE complex; trans-Golgi network; integral component of endosome membrane GO_0031201; GO_0005802; GO_0031303 5588 YOL019W YOL019W Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication C HDA; IDA; HDA fungal-type vacuole; plasma membrane; cell periphery GO_0000324; GO_0005886; GO_0071944 5589 YOL019W-A YOL019W-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5590 YOL020W TAT2 High affinity tryptophan and tyrosine permease High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 5591 YOL021C DIS3 Exosome core complex catalytic subunit Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; has similarity to E. coli RNase R and to human DIS3; mutations in Dis3p corresponding to human mutations implicated in multiple myeloma cause phenotypes suggesting impaired exosome function; protein abundance increases in response to DNA replication stress C HDA; IDA; IDA; HDA; HDA mitochondrion; cytoplasmic exosome (RNase complex); nuclear exosome (RNase complex); nucleus; nucleolus GO_0005739; GO_0000177; GO_0000176; GO_0005634; GO_0005730 5592 YOL022C TSR4 Cytoplasmic protein required for correct processing of 20S pre-rRNA Cytoplasmic protein required for correct processing of 20S pre-rRNA; protein required for processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 C HDA cytoplasm GO_0005737 5593 YOL023W IFM1 Mitochondrial translation initiation factor 2 Mitochondrial translation initiation factor 2 C IMP; HDA; HDA mitochondrion; cell periphery; nucleus GO_0005739; GO_0071944; GO_0005634 5594 YOL024W YOL024W Putative protein of unknown function Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication 5595 YOL025W LAG2 Protein that negatively regulates the SCF E3-ubiquitin ligase Protein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 C HDA mitochondrion GO_0005739 5596 YOL026C MIM1 Mitochondrial protein required for outer membrane protein import Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex C IDA; IDA mitochondrial outer membrane; integral component of mitochondrial outer membrane GO_0005741; GO_0031307 5597 YOL027C MDM38 Mitochondrial protein Mitochondrial protein; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 5598 YOL028C YAP7 Putative basic leucine zipper (bZIP) transcription factor Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication 5599 YOL029C YOL029C Putative protein of unknown function Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens 5600 YOL030W GAS5 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall C IDA; HDA fungal-type cell wall; endoplasmic reticulum GO_0009277; GO_0005783 5601 YOL031C SIL1 Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein C IPI endoplasmic reticulum GO_0005783 5602 YOL032W OPI10 Protein with a possible role in phospholipid biosynthesis Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5603 YOL033W MSE1 Mitochondrial glutamyl-tRNA synthetase Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated C IMP mitochondrion GO_0005739 5604 YOL034W SMC5 Subunit of the SMC5-SMC6 complex Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair C IDA; IDA nucleus; Smc5-Smc6 complex GO_0005634; GO_0030915 5605 YOL035C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5606 YOL036W YOL036W Protein of unknown function Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication 5607 YOL037C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W 5608 YOL038C-A YOL038C-A Putative protein of unknown function Putative protein of unknown function; identified by SAGE analysis 5609 YOL038W PRE6 Alpha 4 subunit of the 20S proteasome Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress C IDA; IDA; IDA; IDA; IDA proteasome storage granule; mitochondrion; nucleus; proteasome core complex, alpha-subunit complex; nuclear outer membrane-endoplasmic reticulum membrane network GO_0034515; GO_0005739; GO_0005634; GO_0019773; GO_0042175 5610 YOL039W RPP2A Ribosomal protein P2 alpha Ribosomal protein P2 alpha; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm C IDA cytosolic large ribosomal subunit GO_0022625 5611 YOL040C RPS15 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 5612 YOL041C NOP12 Nucleolar protein involved in pre-25S rRNA processing Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p C HDA; IDA; IDA preribosome, large subunit precursor; nucleolus; preribosome GO_0030687; GO_0005730; GO_0030684 5613 YOL042W NGL1 Putative endonuclease Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5614 YOL043C NTG2 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication C IDA nucleus GO_0005634 5615 YOL044W PEX15 Tail-anchored type II integral peroxisomal membrane protein Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly C IDA integral component of peroxisomal membrane GO_0005779 5616 YOL045W PSK2 PAS-domain containing serine/threonine protein kinase PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 5617 YOL046C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene PSK2/YOL045W 5618 YOL047C LDS2 Protein Involved in spore wall assembly Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds1p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C HDA; IDA; IDA; IDA; HDA cytoplasm; ascospore wall; ascus lipid particle; prospore membrane; lipid particle GO_0005737; GO_0005619; GO_0005633; GO_0005628; GO_0005811 5619 YOL048C RRT8 Protein involved in spore wall assembly Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress C IDA; HDA; IDA prospore membrane; lipid particle; ascospore wall GO_0005628; GO_0005811; GO_0005619 5620 YOL049W GSH2 Glutathione synthetase Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock 5621 YOL050C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) 5622 YOL051W GAL11 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator C IDA core mediator complex GO_0070847 5623 YOL052C SPE2 S-adenosylmethionine decarboxylase S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5624 YOL052C-A DDR2 Multi-stress response protein Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole; cytoplasm GO_0000324; GO_0005737 5625 YOL053W AIM39 Protein of unknown function Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss C HDA; HDA mitochondrion; cytosol GO_0005739; GO_0005829 5626 YOL054W PSH1 E3 ubiquitin ligase targeting centromere-binding protein Cse4p E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates poyubiquitination and degradation of Cse4p; prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p C IDA; HDA chromosome, centromeric region; nucleus GO_0000775; GO_0005634 5627 YOL055C THI20 Trifunctional enzyme of thiamine biosynthesis, degradation and salvage Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p C IDA cytosol GO_0005829 5628 YOL056W GPM3 Nonfunctional homolog of Gpm1p phosphoglycerate mutase Homolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM3 has a paralog, GPM2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 5629 YOL057W YOL057W Dipeptidyl-peptidase III Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5630 YOL058W ARG1 Arginosuccinate synthetase Arginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate C IDA cytosol GO_0005829 5631 YOL059W GPD2 NAD-dependent glycerol 3-phosphate dehydrogenase NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 5632 YOL060C MAM3 Protein required for normal mitochondrial morphology Protein required for normal mitochondrial morphology; has similarity to hemolysins C HDA; HDA fungal-type vacuole membrane; endoplasmic reticulum GO_0000329; GO_0005783 5633 YOL061W PRS5 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress C HDA; IDA cytoplasm; ribose phosphate diphosphokinase complex GO_0005737; GO_0002189 5634 YOL062C APM4 Cargo-binding mu subunit of AP-2 Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals C HDA; IPI cytosol; AP-2 adaptor complex GO_0005829; GO_0030122 5635 YOL063C CRT10 Protein involved in transcriptional regulation of RNR2 and RNR3 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat 5636 YOL064C MET22 Bisphosphate-3'-nucleotidase Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway 5637 YOL065C INP54 Phosphatidylinositol 4,5-bisphosphate 5-phosphatase Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; role in secretion; localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins C IDA; HDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 5638 YOL066C RIB2 Bifunctional DRAP deaminase tRNA:pseudouridine synthase Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 5639 YOL067C RTG1 Transcription factor (bHLH) involved in interorganelle communication Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 5640 YOL068C HST1 NAD(+)-dependent histone deacetylase NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication C IDA Set3 complex GO_0034967 5641 YOL069W NUF2 Component of the kinetochore-associated Ndc80 complex Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p C IMP; IDA; IDA; IDA; IDA condensed nuclear chromosome kinetochore; spindle microtubule; spindle pole body; condensed nuclear chromosome, centromeric region; Ndc80 complex GO_0000778; GO_0005876; GO_0005816; GO_0000780; GO_0031262 5642 YOL070C NBA1 Protein of unknown function Protein of unknown function; localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate C HDA; HDA; HDA cytoplasm; ribosome; cellular bud neck GO_0005737; GO_0005840; GO_0005935 5643 YOL071W SDH5 Protein required for flavinylation of Sdh1p Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 5644 YOL072W THP1 Nuclear pore-associated protein Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding C IGI; IDA; IDA; IDA nuclear pore; transcription export complex 2; nucleus; nuclear envelope GO_0005643; GO_0070390; GO_0005634; GO_0005635 5645 YOL073C DSC2 Multi-transmembrane subunit of the DSC ubiquitin ligase complex Multi-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2 C IDA; HDA Dsc E3 ubiquitin ligase complex; endoplasmic reticulum GO_0044695; GO_0005783 5646 YOL075C YOL075C Putative ABC transporter Putative ABC transporter C IDA; HDA fungal-type vacuole membrane; cell periphery GO_0000329; GO_0071944 5647 YOL076W MDM20 Non-catalytic subunit of the NatB N-terminal acetyltransferase Non-catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly C IDA NatB complex GO_0031416 5648 YOL077C BRX1 Nucleolar protein Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif C HDA; IDA preribosome, large subunit precursor; nucleolus GO_0030687; GO_0005730 5649 YOL077W-A ATP19 Subunit k of the mitochondrial F1F0 ATP synthase Subunit k of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase C HDA; IPI mitochondrion; mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) GO_0005739; GO_0000276 5650 YOL078W AVO1 Component of a membrane-bound complex containing the Tor2p kinase Component of a membrane-bound complex containing the Tor2p kinase; contains Tor2p kinase and other proteins; may have a role in regulation of cell growth C IDA; IPI; IPI plasma membrane; cytoplasm; TORC2 complex GO_0005886; GO_0005737; GO_0031932 5651 YOL079W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5652 YOL080C REX4 Putative RNA exonuclease Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly C HDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 5653 YOL081W IRA2 GTPase-activating protein GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA mitochondrion; cytosol; cytoplasm; endoplasmic reticulum membrane GO_0005739; GO_0005829; GO_0005737; GO_0005789 5654 YOL082W ATG19 Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles C IDA; IMP; HDA; IDA; IDA extrinsic component of membrane; CVT complex; cytosol; cytoplasm; pre-autophagosomal structure GO_0019898; GO_0034270; GO_0005829; GO_0005737; GO_0000407 5655 YOL083C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry 5656 YOL083W ATG34 Receptor protein involved in selective autophagy during starvation Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog C IDA; HDA CVT complex; cytosol GO_0034270; GO_0005829 5657 YOL084W PHM7 Protein of unknown function Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress C HDA; HDA endoplasmic reticulum; fungal-type vacuole GO_0005783; GO_0000324 5658 YOL085C YOL085C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A 5659 YOL085W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YOL085C 5660 YOL086C ADH1 Alcohol dehydrogenase Alcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication C IDA; HDA cytoplasm; plasma membrane GO_0005737; GO_0005886 5661 YOL086W-A MHF1 Component of the heterotetrameric MHF histone-fold complex Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 C IDA FANCM-MHF complex GO_0071821 5662 YOL087C DUF1 Ubiquitin-binding protein of unknown function Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid C HDA cytoplasm GO_0005737 5663 YOL088C MPD2 Member of the protein disulfide isomerase (PDI) family Member of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p C HDA endoplasmic reticulum GO_0005783 5664 YOL089C HAL9 Putative transcription factor containing a zinc finger Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 5665 YOL090W MSH2 Protein that binds to DNA mismatches Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP C IDA; HDA; IPI; IPI nuclear chromosome; nucleus; MutSalpha complex; MutSbeta complex GO_0000228; GO_0005634; GO_0032301; GO_0032302 5666 YOL091W SPO21 Component of the meiotic outer plaque of the spindle pole body Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation; SPO21 has a paralog, YSW1, that arose from the whole genome duplication C IDA; HDA meiotic spindle pole body; spindle pole body GO_0035974; GO_0005816 5667 YOL092W YPQ1 Putative vacuolar membrane transporter for cationic amino acids Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication C IDA; HDA; HDA; HDA integral component of fungal-type vacuolar membrane; fungal-type vacuole membrane; endoplasmic reticulum; cytoplasm GO_0071627; GO_0000329; GO_0005783; GO_0005737 5668 YOL093W TRM10 tRNA methyltransferase tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5669 YOL094C RFC4 Subunit of heteropentameric Replication factor C (RF-C) Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA; IDA; IPI; IDA Elg1 RFC-like complex; nucleus; Rad17 RFC-like complex; cytosol; Ctf18 RFC-like complex; DNA replication factor C complex GO_0031391; GO_0005634; GO_0031389; GO_0005829; GO_0031390; GO_0005663 5670 YOL095C HMI1 Mitochondrial inner membrane localized ATP-dependent DNA helicase Mitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 5671 YOL096C COQ3 O-methyltransferase O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein C HDA; IDA; IDA mitochondrion; mitochondrial inner membrane; extrinsic component of membrane GO_0005739; GO_0005743; GO_0019898 5672 YOL097C WRS1 Cytoplasmic tryptophanyl-tRNA synthetase Cytoplasmic tryptophanyl-tRNA synthetase; aminoacylates tryptophanyl-tRNA C HDA cytoplasm GO_0005737 5673 YOL097W-A YOL097W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 5674 YOL098C SDD3 Putative metalloprotease Putative metalloprotease C HDA cytoplasm GO_0005737 5675 YOL099C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes 5676 YOL100W PKH2 Serine/threonine protein kinase Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication C IDA; IDA; IDA eisosome; nucleus; cell cortex GO_0032126; GO_0005634; GO_0005938 5677 YOL101C IZH4 Membrane protein involved in zinc ion homeostasis Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication C HDA; HDA; HDA fungal-type vacuole; cytoplasm; nucleus GO_0000324; GO_0005737; GO_0005634 5678 YOL102C TPT1 tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 5679 YOL103W ITR2 Myo-inositol transporter Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication C IDA; HDA; HDA plasma membrane; fungal-type vacuole; cell periphery GO_0005886; GO_0000324; GO_0071944 5680 YOL103W-A YOL103W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 5681 YOL103W-B YOL103W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 5682 YOL104C NDJ1 Protein that regulates meiotic SPB cohesion and telomere clustering Meiosis-specific telomere protein; required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement C IDA; IDA nuclear chromosome, telomeric region; meiotic spindle pole body GO_0000784; GO_0035974 5683 YOL105C WSC3 Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication C HDA; HDA mating projection tip; fungal-type vacuole GO_0043332; GO_0000324 5684 YOL106W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5685 YOL107W YOL107W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein C HDA; HDA Golgi apparatus; COPI-coated vesicle GO_0005794; GO_0030137 5686 YOL108C INO4 Transcription factor involved in phospholipid synthesis Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain C IDA; IPI nucleus; RNA polymerase II transcription factor complex GO_0005634; GO_0090575 5687 YOL109W ZEO1 Peripheral membrane protein of the plasma membrane Peripheral membrane protein of the plasma membrane; interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria C HDA; IDA; HDA; HDA mitochondrion; extrinsic component of plasma membrane; plasma membrane; mitochondrial outer membrane GO_0005739; GO_0019897; GO_0005886; GO_0005741 5688 YOL110W SHR5 Palmitoyltransferase subunit Palmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2 C IPI; IDA; HDA endoplasmic reticulum palmitoyltransferase complex; extrinsic component of endoplasmic reticulum membrane; endoplasmic reticulum GO_0031211; GO_0042406; GO_0005783 5689 YOL111C MDY2 Protein involved in inserting tail-anchored proteins into ER membranes Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes C IDA; HDA; IDA; IDA; IDA nucleus; cytoplasm; cytoplasmic stress granule; ribosome; TRC complex GO_0005634; GO_0005737; GO_0010494; GO_0005840; GO_0072380 5690 YOL112W MSB4 GTPase-activating protein of the Ras superfamily GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication C IDA; IDA; IDA polarisome; cellular bud tip; incipient cellular bud site GO_0000133; GO_0005934; GO_0000131 5691 YOL113W SKM1 Member of the PAK family of serine/threonine protein kinases Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication C IMP; IDA nucleus; plasma membrane GO_0005634; GO_0005886 5692 YOL114C PTH4 Protein similar to the human peptidyl-tRNA hydrolase gene ICT1 Putative protein of unknown function with similarity to human ICT1; has prokaryotic factors that may function in translation termination; YOL114C is not an essential gene C IDA mitochondrial large ribosomal subunit GO_0005762 5693 YOL115W PAP2 Non-canonical poly(A) polymerase Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia C IDA; IDA; IDA; IDA nucleus; TRAMP complex; nucleolus; cytosol GO_0005634; GO_0031499; GO_0005730; GO_0005829 5694 YOL116W MSN1 Transcriptional activator Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5695 YOL117W RRI2 Subunit of the COP9 signalosome (CSN) complex Subunit of the COP9 signalosome (CSN) complex; this complex cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response C IDA COP9 signalosome GO_0008180 5696 YOL118C YOL118C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5697 YOL119C MCH4 Protein with similarity to mammalian monocarboxylate permeases Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport C IDA; HDA fungal-type vacuole membrane; cell periphery GO_0000329; GO_0071944 5698 YOL120C RPL18A Ribosomal 60S subunit protein L18A Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 5699 YOL121C RPS19A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 5700 YOL122C SMF1 Divalent metal ion transporter Divalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins C HDA; IDA fungal-type vacuole; plasma membrane GO_0000324; GO_0005886 5701 YOL123W HRP1 Subunit of cleavage factor I Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability C IDA; IDA; IPI; IDA cytoplasm; nucleus; mRNA cleavage factor complex; cytoplasmic stress granule GO_0005737; GO_0005634; GO_0005849; GO_0010494 5702 YOL124C TRM11 Catalytic subunit of adoMet-dependent tRNA methyltransferase complex Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p C IDA; IPI cytoplasm; tRNA (m2G10) methyltransferase complex GO_0005737; GO_0043528 5703 YOL125W TRM13 2'-O-methyltransferase 2'-O-methyltransferase; responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5704 YOL126C MDH2 Cytoplasmic malate dehydrogenase Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 C IDA; IDA; HDA cytosol; nuclear periphery; cytoplasm GO_0005829; GO_0034399; GO_0005737 5705 YOL127W RPL25 Ribosomal 60S subunit protein L25 Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 C IDA; IDA preribosome, large subunit precursor; cytosolic large ribosomal subunit GO_0030687; GO_0022625 5706 YOL128C YGK3 Protein kinase related to mammalian GSK-3 glycogen synthase kinases Protein kinase related to mammalian GSK-3 glycogen synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation; YGK3 has a paralog, MCK1, that arose from the whole genome duplication 5707 YOL129W VPS68 Vacuolar membrane protein of unknown function Vacuolar membrane protein of unknown function; involved in vacuolar protein sorting; also detected in the mitochondria C HDA; HDA; IDA mitochondrion; fungal-type vacuole membrane; Vps55/Vps68 complex GO_0005739; GO_0000329; GO_0034424 5708 YOL130W ALR1 Plasma membrane Mg(2+) transporter Plasma membrane Mg(2+) transporter; expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 5709 YOL131W YOL131W Putative protein of unknown function Putative protein of unknown function; YOL131W has a paralog, STB1, that arose from the whole genome duplication 5710 YOL132W GAS4 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall C HDA; IDA fungal-type vacuole; fungal-type cell wall GO_0000324; GO_0009277 5711 YOL133W HRT1 RING-H2 domain core subunit of multiple ubiquitin ligase complexes RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair C IPI; IDA; IDA; IDA; IDA SCF ubiquitin ligase complex; Cul3-RING ubiquitin ligase complex; Cul8-RING ubiquitin ligase complex; cytoplasm; nucleus GO_0019005; GO_0031463; GO_0035361; GO_0005737; GO_0005634 5712 YOL134C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit 5713 YOL135C MED7 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C IDA core mediator complex GO_0070847 5714 YOL136C PFK27 6-phosphofructo-2-kinase 6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A 5715 YOL137W BSC6 Protein of unknown function with 8 putative transmembrane segments Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression C HDA; HDA clathrin-coated vesicle; COPI-coated vesicle GO_0030136; GO_0030137 5716 YOL138C RTC1 Subunit of SEACAT, a subcomplex of the SEA complex Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif C IDA; HDA; HDA; HDA; IDA Seh1-associated complex; cytosol; ribosome; fungal-type vacuole; extrinsic component of fungal-type vacuolar membrane GO_0035859; GO_0005829; GO_0005840; GO_0000324; GO_0097042 5717 YOL139C CDC33 mRNA cap binding protein and translation initiation factor eIF4E mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth C IDA; IDA; IDA; IDA cytoplasmic stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; nucleus GO_0010494; GO_0005737; GO_0016281; GO_0005634 5718 YOL140W ARG8 Acetylornithine aminotransferase Acetylornithine aminotransferase; catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 5719 YOL141W PPM2 AdoMet-dependent tRNA methyltransferase AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 5720 YOL142W RRP40 Exosome non-catalytic core component Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain both S1 and KH RNA binding domains; has similarity to human hRrp40p (EXOSC3) C IDA; IDA nuclear exosome (RNase complex); cytoplasmic exosome (RNase complex) GO_0000176; GO_0000177 5721 YOL143C RIB4 Lumazine synthase (DMRL synthase) Lumazine synthase (DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin; DMRL synthase stands for 6,7-dimethyl-8-ribityllumazine synthase C IDA; HDA; HDA mitochondrial intermembrane space; nucleus; cytoplasm GO_0005758; GO_0005634; GO_0005737 5722 YOL144W NOP8 Nucleolar protein required for 60S ribosomal subunit biogenesis Nucleolar protein required for 60S ribosomal subunit biogenesis C IDA nucleolus GO_0005730 5723 YOL145C CTR9 Component of the Paf1p complex involved in transcription elongation Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats C IPI; IDA; IDA Cdc73/Paf1 complex; transcriptionally active chromatin; nucleus GO_0016593; GO_0035327; GO_0005634 5724 YOL146W PSF3 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery C IPI; IDA; IPI; IDA GINS complex; CMG complex; DNA replication preinitiation complex; replication fork protection complex GO_0000811; GO_0071162; GO_0031261; GO_0031298 5725 YOL147C PEX11 Peroxisomal protein required for medium-chain fatty acid oxidation Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p C IDA; IDA; IDA peroxisomal membrane; peroxisomal importomer complex; endoplasmic reticulum GO_0005778; GO_1990429; GO_0005783 5726 YOL148C SPT20 Subunit of the SAGA transcriptional regulatory complex Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay C IDA; IDA SAGA complex; SLIK (SAGA-like) complex GO_0000124; GO_0046695 5727 YOL149W DCP1 Subunit of the Dcp1p-Dcp2p decapping enzyme complex Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress C IDA; IDA; HDA; IPI; IDA nucleus; P-body; cytoplasm; Dcp1-Dcp2 complex; cytoplasmic side of membrane GO_0005634; GO_0000932; GO_0005737; GO_0098745; GO_0098562 5728 YOL150C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5729 YOL151W GRE2 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 5730 YOL152W FRE7 Putative ferric reductase with similarity to Fre2p Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels C IDA plasma membrane GO_0005886 5731 YOL154W ZPS1 Putative GPI-anchored protein Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH C HDA; IDA; HDA cell periphery; fungal-type cell wall; fungal-type vacuole GO_0071944; GO_0009277; GO_0000324 5732 YOL155C HPF1 Haze-protective mannoprotein Haze-protective mannoprotein; reduces the particle size of aggregated proteins in white wines C HDA; IDA; IDA cell periphery; extracellular region; fungal-type cell wall GO_0071944; GO_0005576; GO_0009277 5733 YOL155W-A YOL155W-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 5734 YOL156W HXT11 Hexose transporter Putative hexose transporter that is nearly identical to Hxt9p; has similarity to major facilitator superfamily (MFS) transporters and is involved in pleiotropic drug resistance C HDA cell periphery GO_0071944 5735 YOL157C IMA2 Isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase) Isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; not required for isomaltose utilization, but Ima2p overexpression allows the ima1 null mutant to grow on isomaltose 5736 YOL158C ENB1 Endosomal ferric enterobactin transporter Endosomal ferric enterobactin transporter; expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment C IDA; HDA; IDA; HDA plasma membrane; cell periphery; cellular bud membrane; fungal-type vacuole GO_0005886; GO_0071944; GO_0033101; GO_0000324 5737 YOL159C CSS3 Protein of unknown function, secreted when constitutively expressed Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA C HDA; HDA extracellular region; cell periphery GO_0005576; GO_0071944 5738 YOL159C-A YOL159C-A Protein of unknown function Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5739 YOL160W YOL160W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5740 YOL161C PAU20 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression induced by low temperature and also by anaerobic conditions; induced during alcoholic fermentation C HDA fungal-type vacuole GO_0000324 5741 YOL162W YOL162W Putative protein of unknown function Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family C HDA endoplasmic reticulum GO_0005783 5742 YOL163W YOL163W Putative protein of unknown function Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family 5743 YOL164W BDS1 Bacterially-derived sulfatase Bacterially-derived sulfatase; required for use of alkyl- and aryl-sulfates as sulfur sources 5744 YOL164W-A YOL164W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 5745 YOL165C AAD15 Putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD15 has a paralog, AAD3, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family 5746 YOL166C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5747 YOL166W-A YOL166W-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5748 YOR001W RRP6 Nuclear exosome exonuclease component Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based C IDA; IDA; IDA nucleus; nuclear exosome (RNase complex); nucleolus GO_0005634; GO_0000176; GO_0005730 5749 YOR002W ALG6 Alpha 1,3 glucosyltransferase Alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease C HDA endoplasmic reticulum GO_0005783 5750 YOR003W YSP3 Putative precursor of the subtilisin-like protease III Putative precursor to the subtilisin-like protease III; YSP3 has a paralog, PRB1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5751 YOR004W UTP23 Component of the small subunit processome Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein C IPI; HDA; IDA small-subunit processome; mitochondrion; nucleolus GO_0032040; GO_0005739; GO_0005730 5752 YOR005C DNL4 DNA ligase required for nonhomologous end-joining (NHEJ) DNA ligase required for nonhomologous end-joining (NHEJ); forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth C IPI; IPI nuclear chromatin; DNA ligase IV complex GO_0000790; GO_0032807 5753 YOR006C TSR3 Protein required for 20S pre-rRNA processing Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5754 YOR007C SGT2 Glutamine-rich cytoplasmic cochaperone Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress C HDA; IDA cytoplasm; TRC complex GO_0005737; GO_0072380 5755 YOR008C SLG1 Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response C IDA; IDA; HDA; HDA; HDA cellular bud neck; plasma membrane; mating projection tip; fungal-type vacuole; endoplasmic reticulum GO_0005935; GO_0005886; GO_0043332; GO_0000324; GO_0005783 5756 YOR008C-A YOR008C-A Putative protein of unknown function Putative protein of unknown function; includes a potential transmembrane domain; deletion results in slightly lengthened telomeres 5757 YOR008W-B Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5758 YOR009W TIR4 Cell wall mannoprotein Cell wall mannoprotein; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins C HDA; HDA; IDA cell periphery; fungal-type vacuole; fungal-type cell wall GO_0071944; GO_0000324; GO_0009277 5759 YOR010C TIR2 Putative cell wall mannoprotein Putative cell wall mannoprotein; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis; TIR2 has a paralog, TIR3, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 5760 YOR011W AUS1 Plasma membrane sterol transporter of the ATP-binding cassette family Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication C HDA; IDA; HDA mitochondrion; plasma membrane; cell periphery GO_0005739; GO_0005886; GO_0071944 5761 YOR011W-A YOR011W-A Putative protein of unknown function Putative protein of unknown function 5762 YOR012W YOR012W Putative protein of unknown function Putative protein of unknown function 5763 YOR013W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci 5764 YOR014W RTS1 B-type regulatory subunit of protein phosphatase 2A (PP2A) B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A C IDA; IDA; IDA; HDA; IDA; IDA; IDA kinetochore; nucleus; spindle pole body; cytoplasm; cellular bud neck; condensed nuclear chromosome, centromeric region; chromosome, centromeric region GO_0000776; GO_0005634; GO_0005816; GO_0005737; GO_0005935; GO_0000780; GO_0000775 5765 YOR015W YOR015W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5766 YOR016C ERP4 Member of the p24 family involved in ER to Golgi transport Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5767 YOR017W PET127 Protein with a role in 5'-end processing of mitochondrial RNAs Protein with a role in 5'-end processing of mitochondrial RNAs; located in the mitochondrial membrane C HDA; IDA mitochondrion; mitochondrial envelope GO_0005739; GO_0005740 5768 YOR018W ROD1 Alpha-arrestin involved in ubiquitin-dependent endocytosis Alpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication C HDA; IDA cytosol; plasma membrane GO_0005829; GO_0005886 5769 YOR019W YOR019W Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; YOR019W has a paralog, JIP4, that arose from the whole genome duplication C HDA ribosome GO_0005840 5770 YOR020C HSP10 Mitochondrial matrix co-chaperonin Mitochondrial matrix co-chaperonin; inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 5771 YOR020W-A MCO10 Putative protein of unknown function Putative protein of unknown function; conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 5772 YOR021C SFM1 SPOUT methyltransferase SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism 5773 YOR022C DDL1 DDHD domain-containing phospholipase A1 Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 5774 YOR023C AHC1 Subunit of the Ada histone acetyltransferase complex Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex C IDA Ada2/Gcn5/Ada3 transcription activator complex GO_0005671 5775 YOR024W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5776 YOR025W HST3 Member of the Sir2 family of NAD(+)-dependent protein deacetylases Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism 5777 YOR026W BUB3 Kinetochore checkpoint WD40 repeat protein Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p C IPI; IDA mitotic checkpoint complex; condensed nuclear chromosome kinetochore GO_0033597; GO_0000778 5778 YOR027W STI1 Hsp90 cochaperone Hsp90 cochaperone; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop C IPI cytoplasm GO_0005737 5779 YOR028C CIN5 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 5780 YOR029W YOR029W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5781 YOR030W DFG16 Probable multiple transmembrane protein Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p C IDA plasma membrane GO_0005886 5782 YOR031W CRS5 Copper-binding metallothionein Copper-binding metallothionein; required for wild-type copper resistance 5783 YOR032C HMS1 bHLH protein with similarity to myc-family transcription factors bHLH protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant 5784 YOR032W-A YOR032W-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching C HDA endoplasmic reticulum GO_0005783 5785 YOR033C EXO1 5'-3' exonuclease and flap-endonuclease 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication C HDA; IPI cytoplasm; nucleus GO_0005737; GO_0005634 5786 YOR034C AKR2 Ankyrin repeat-containing protein Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication C HDA; HDA; IDA fungal-type vacuole membrane; fungal-type vacuole; Golgi apparatus GO_0000329; GO_0000324; GO_0005794 5787 YOR034C-A YOR034C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 5788 YOR035C SHE4 Protein containing a UCS (UNC-45/CRO1/SHE4) domain Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization C IDA cytoplasm GO_0005737 5789 YOR036W PEP12 Target membrane receptor (t-SNARE) Target membrane receptor (t-SNARE); for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin C HDA; IMP; IMP fungal-type vacuole membrane; Golgi apparatus; endosome GO_0000329; GO_0005794; GO_0005768 5790 YOR037W CYC2 Mitochondrial peripheral inner membrane protein Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) C IDA; HDA extrinsic component of mitochondrial inner membrane; mitochondrion GO_0031314; GO_0005739 5791 YOR038C HIR2 Subunit of HIR nucleosome assembly complex Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; nucleus; HIR complex GO_0005829; GO_0005634; GO_0000417 5792 YOR039W CKB2 Beta' regulatory subunit of casein kinase 2 (CK2) Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase C IDA; IDA; IDA UTP-C complex; protein kinase CK2 complex; CURI complex GO_0034456; GO_0005956; GO_0032545 5793 YOR040W GLO4 Mitochondrial glyoxalase II Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 5794 YOR041C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance 5795 YOR042W CUE5 Ubiquitin-binding protein Ubiquitin-binding protein; functions as a ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; contains a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; GFP-fusion protein localizes to the cytoplasm in a punctate pattern; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q C HDA cytoplasm GO_0005737 5796 YOR043W WHI2 Protein required for full activation of the general stress response Protein required for full activation of the general stress response; required with binding partner Psr1p, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression C HDA cell periphery GO_0071944 5797 YOR044W IRC23 Protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 5798 YOR045W TOM6 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex C IMP; HDA mitochondrial outer membrane translocase complex; mitochondrion GO_0005742; GO_0005739 5799 YOR046C DBP5 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress C IDA; HDA; IDA; IDA; IDA; IDA; IPI; IDA nuclear pore cytoplasmic filaments; preribosome, large subunit precursor; cytoplasm; polysome; cellular bud tip; cytoplasmic stress granule; nuclear pore; nucleus GO_0044614; GO_0030687; GO_0005737; GO_0005844; GO_0005934; GO_0010494; GO_0005643; GO_0005634 5800 YOR047C STD1 Protein involved in control of glucose-regulated gene expression Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]; STD1 has a paralog, MTH1, that arose from the whole genome duplication C IDA; IDA plasma membrane; nucleus GO_0005886; GO_0005634 5801 YOR048C RAT1 Nuclear 5' to 3' single-stranded RNA exonuclease Nuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage C HDA; HDA nucleus; mitochondrion GO_0005634; GO_0005739 5802 YOR049C RSB1 Putative sphingoid long-chain base (LCB) efflux transporter Suppressor of sphingoid LCB sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport long chain bases (LCBs) from the cytoplasmic side toward the extracytoplasmic side of the membrane C HDA; IDA; IDA; IDA; IDA fungal-type vacuole; plasma membrane; endoplasmic reticulum; cell periphery; integral component of membrane GO_0000324; GO_0005886; GO_0005783; GO_0071944; GO_0016021 5803 YOR050C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant is viable 5804 YOR051C ETT1 Nuclear protein that inhibits replication of Brome mosaic virus Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough C HDA nucleus GO_0005634 5805 YOR052C TMC1 AN1-type zinc finger protein, effector of proteotoxic stress response AN1-type zinc finger protein of unknown function; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element, a transcriptional profile similar to CUZ1 and RPN2, and decreased expression in an RPN4 mutant; induced by nitrogen limitation and weak acid; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress C HDA nucleus GO_0005634 5806 YOR053W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C 5807 YOR054C VHS3 Negative regulatory subunit of protein phosphatase 1 Ppz1p Negative regulatory subunit of protein phosphatase 1 Ppz1p; involved in coenzyme A biosynthesis; subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p) C IDA; IDA phosphopantothenoylcysteine decarboxylase complex; CoA-synthesizing protein complex GO_0071513; GO_1990143 5808 YOR055W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5809 YOR056C NOB1 Protein involved in proteasomal and 40S ribosomal subunit biogenesis Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress C IDA; IDA; IDA cytoplasm; nucleus; preribosome, small subunit precursor GO_0005737; GO_0005634; GO_0030688 5810 YOR057W SGT1 Cochaperone protein Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress C IDA ubiquitin ligase complex GO_0000151 5811 YOR058C ASE1 Mitotic spindle midzone-localized microtubule bundling protein Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress C HDA; IDA; IDA; HDA; IDA cytoplasm; spindle midzone; nuclear microtubule; microtubule; spindle GO_0005737; GO_0051233; GO_0005880; GO_0005874; GO_0005819 5812 YOR059C LPL1 Phospholipase Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress C IDA; HDA lipid particle; endoplasmic reticulum GO_0005811; GO_0005783 5813 YOR060C SLD7 Protein with a role in chromosomal DNA replication Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria C HDA; HDA; HDA; HDA; IDA; IDA; HDA nucleus; spindle pole body; nuclear envelope; endoplasmic reticulum; chromosome, centromeric region; DNA replication preinitiation complex; cytoplasm GO_0005634; GO_0005816; GO_0005635; GO_0005783; GO_0000775; GO_0031261; GO_0005737 5814 YOR061W CKA2 Alpha' catalytic subunit of casein kinase 2 (CK2) Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching C IDA; IDA UTP-C complex; protein kinase CK2 complex GO_0034456; GO_0005956 5815 YOR062C YOR062C Protein of unknown function Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5816 YOR063W RPL3 Ribosomal 60S subunit protein L3 Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; plays an important role in function of eIF5B in stimulating 3' end processing of 18S rRNA in context of 80S ribosomes that have not yet engaged in translation; involved in replication and maintenance of killer double stranded RNA virus C HDA; IDA preribosome, large subunit precursor; cytosolic large ribosomal subunit GO_0030687; GO_0022625 5817 YOR064C YNG1 Subunit of the NuA3 histone acetyltransferase complex Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 C IDA; IDA NuA3 histone acetyltransferase complex; NuA3a histone acetyltransferase complex GO_0033100; GO_1990467 5818 YOR065W CYT1 Cytochrome c1 Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex C IDA; HDA mitochondrial respiratory chain complex III; mitochondrion GO_0005750; GO_0005739 5819 YOR066W MSA1 Activator of G1-specific transcription factors MBF and SBF Activator of G1-specific transcription factors MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that arose from the whole genome duplication C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 5820 YOR067C ALG8 Glucosyl transferase Glucosyl transferase; involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p C HDA; IMP endoplasmic reticulum; endoplasmic reticulum membrane GO_0005783; GO_0005789 5821 YOR068C VAM10 Protein involved in vacuole morphogenesis Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering 5822 YOR069W VPS5 Nexin-1 homolog Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication C IDA; IPI; IPI; IDA endosome; retromer, tubulation complex; retromer complex; cytosol GO_0005768; GO_0030905; GO_0030904; GO_0005829 5823 YOR070C GYP1 Cis-golgi GTPase-activating protein (GAP) for yeast Rabs Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion C IDA; HDA Golgi apparatus; mitochondrion GO_0005794; GO_0005739 5824 YOR071C NRT1 High-affinity nicotinamide riboside transporter High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; major transporter for 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine) uptake; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity 5825 YOR072W YOR072W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive 5826 YOR072W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR072W; originally identified by fungal homology and RT-PCR 5827 YOR072W-B YOR072W-B Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 5828 YOR073W SGO1 Component of the spindle checkpoint Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension C IDA; HDA; IDA; IDA; HDA chromosome, centromeric region; spindle pole body; condensed nuclear chromosome, centromeric region; condensed nuclear chromosome kinetochore; nucleus GO_0000775; GO_0005816; GO_0000780; GO_0000778; GO_0005634 5829 YOR073W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CDC21/YOR074C; identified by RT-PCR 5830 YOR074C CDC21 Thymidylate synthase Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S C IDA nuclear periphery GO_0034399 5831 YOR075W UFE1 t-SNARE protein required for retrograde vesicular traffic t-SNARE protein required for retrograde vesicular traffic; also required for homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER C HDA; IDA endoplasmic reticulum; SNARE complex GO_0005783; GO_0031201 5832 YOR076C SKI7 GTP-binding protein that couples the Ski complex and exosome Coupling protein for the Ski complex and cytoplasmic exosome; involved in 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication C IDA; HDA; IDA; IDA cytoplasmic exosome (RNase complex); cytosol; cytoplasm; Ski complex GO_0000177; GO_0005829; GO_0005737; GO_0055087 5833 YOR077W RTS2 Basic zinc-finger protein Basic zinc-finger protein; similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5834 YOR078W BUD21 Component of small ribosomal subunit (SSU) processosome Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern C IDA; IDA; HDA small-subunit processome; nucleolus; 90S preribosome GO_0032040; GO_0005730; GO_0030686 5835 YOR079C ATX2 Golgi membrane protein involved in manganese homeostasis Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation C IDA; HDA; IDA; IDA Golgi membrane; endoplasmic reticulum; late endosome; trans-Golgi network GO_0000139; GO_0005783; GO_0005770; GO_0005802 5836 YOR080W DIA2 Origin-binding F-box protein Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitylation of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth C IDA; IDA; IDA nuclear replication fork; SCF ubiquitin ligase complex; nucleus GO_0043596; GO_0019005; GO_0005634 5837 YOR081C TGL5 Bifunctional triacylglycerol lipase and LPA acyltransferase Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication C IDA lipid particle GO_0005811 5838 YOR082C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR083W 5839 YOR083W WHI5 Repressor of G1 transcription Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1; WHI5 has a paralog, SRL3, that arose from the whole genome duplication C IDA; HDA; IDA cytoplasm; nucleus; SBF transcription complex GO_0005737; GO_0005634; GO_0033309 5840 YOR084W LPX1 Peroxisomal matrix-localized lipase Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible C IDA peroxisomal matrix GO_0005782 5841 YOR085W OST3 Gamma subunit of the oligosaccharyltransferase complex of the ER lumen Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins C IPI; IMP; HDA oligosaccharyltransferase complex; integral component of membrane; endoplasmic reticulum GO_0008250; GO_0016021; GO_0005783 5842 YOR086C TCB1 Lipid-binding ER protein involved in ER-plasma membrane tethering Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in PM; contains 3 calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact; TCB1 has a paralog, TCB2, that arose from the whole genome duplication C HDA; HDA; HDA; IDA; HDA endoplasmic reticulum; mitochondrion; cell periphery; cortical endoplasmic reticulum; plasma membrane GO_0005783; GO_0005739; GO_0071944; GO_0032541; GO_0005886 5843 YOR087W YVC1 Vacuolar cation channel Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock C IDA; IDA; IDA fungal-type vacuole; fungal-type vacuole membrane; vacuole-mitochondrion membrane contact site GO_0000324; GO_0000329; GO_1990816 5844 YOR089C VPS21 Endosomal Rab family GTPase Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication C HDA; HDA; IMP mitochondrial outer membrane; mitochondrion; late endosome GO_0005741; GO_0005739; GO_0005770 5845 YOR090C PTC5 Mitochondrial type 2C protein phosphatase (PP2C) Mitochondrial type 2C protein phosphatase (PP2C); involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p C IDA; IDA; IDA mitochondrion; mitochondrial pyruvate dehydrogenase complex; mitochondrial intermembrane space GO_0005739; GO_0005967; GO_0005758 5846 YOR091W TMA46 Protein of unknown function that associates with translating ribosomes Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p C IDA; IDA; IDA ribosome; polysomal ribosome; cytoplasm GO_0005840; GO_0042788; GO_0005737 5847 YOR092W ECM3 Non-essential protein of unknown function Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication C HDA; HDA cytoplasm; endoplasmic reticulum GO_0005737; GO_0005783 5848 YOR093C CMR2 Putative protein of unknown function Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents C HDA; HDA cellular bud neck; cytosol GO_0005935; GO_0005829 5849 YOR094W ARF3 Glucose-repressible ADP-ribosylation factor Glucose-repressible ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulating cell polarity and invasive growth; also has mRNA binding activity C IDA; HDA; IDA cellular bud tip; cytosol; cellular bud neck GO_0005934; GO_0005829; GO_0005935 5850 YOR095C RKI1 Ribose-5-phosphate ketol-isomerase Ribose-5-phosphate ketol-isomerase; catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5851 YOR096W RPS7A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication C HDA; IDA 90S preribosome; small-subunit processome GO_0030686; GO_0032040 5852 YOR097C YOR097C Putative protein of unknown function Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene 5853 YOR098C NUP1 FG-nucleoporin component of central core of the nuclear pore complex FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 C IDA; IDA; IDA nuclear pore; nuclear pore central transport channel; nuclear pore nuclear basket GO_0005643; GO_0044613; GO_0044615 5854 YOR099W KTR1 Alpha-1,2-mannosyltransferase Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress C HDA; HDA; IDA fungal-type vacuole membrane; fungal-type vacuole; Golgi apparatus GO_0000329; GO_0000324; GO_0005794 5855 YOR100C CRC1 Mitochondrial inner membrane carnitine transporter Mitochondrial inner membrane carnitine transporter; required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5856 YOR101W RAS1 GTPase involved in G-protein signaling in adenylate cyclase activation GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication C HDA; HDA; IDA; HDA cytoplasm; nucleus; plasma membrane; cell periphery GO_0005737; GO_0005634; GO_0005886; GO_0071944 5857 YOR102W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex 5858 YOR103C OST2 Epsilon subunit of the oligosaccharyltransferase complex Epsilon subunit of the oligosaccharyltransferase complex; located in the ER lumen; catalyzes asparagine-linked glycosylation of newly synthesized proteins C IPI; HDA oligosaccharyltransferase complex; endoplasmic reticulum GO_0008250; GO_0005783 5859 YOR104W PIN2 Exomer-dependent cargo protein Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated C IDA; HDA; IDA plasma membrane; fungal-type vacuole membrane; cellular bud neck GO_0005886; GO_0000329; GO_0005935 5860 YOR105W YOR105W Protein of unknown function Protein of unknown function; expressed at both mRNA and protein levels 5861 YOR106W VAM3 Syntaxin-like vacuolar t-SNARE Syntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region C IDA; IDA fungal-type vacuole membrane; SNARE complex GO_0000329; GO_0031201 5862 YOR107W RGS2 Negative regulator of glucose-induced cAMP signaling Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5863 YOR108C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5864 YOR108W LEU9 Alpha-isopropylmalate synthase II (2-isopropylmalate synthase) Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 5865 YOR109W INP53 Polyphosphatidylinositol phosphatase Polyphosphatidylinositol phosphatase; dephosphorylates multiple phosphatidylinositol phosphates; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; INP53 has a paralog, INP52, that arose from the whole genome duplication C IDA; IDA; IDA actin cortical patch; membrane; cytoplasm GO_0030479; GO_0016020; GO_0005737 5866 YOR110W TFC7 RNA pol III transcription initiation factor complex (TFIIIC) subunit RNA pol III transcription initiation factor complex (TFIIIC) subunit; part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; TFC7 has a paralog, YNL108C, that arose from the whole genome duplication C IDA transcription factor TFIIIC complex GO_0000127 5867 YOR111W YOR111W Putative protein of unknown function Putative protein of unknown function 5868 YOR112W CEX1 Component of nuclear aminoacylation-dependent tRNA export pathway Component of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex C IPI; IDA nuclear pore; cytoplasm GO_0005643; GO_0005737 5869 YOR113W AZF1 Zinc-finger transcription factor Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia C IDA; IDA nucleus; cytosol GO_0005634; GO_0005829 5870 YOR114W DPI34 Putative protein of unknown function Putative protein of unknown function; null mutant is viable 5871 YOR115C TRS33 Core component of TRAPP complexes I, II and IV Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) C IDA; IDA; HDA; IDA; IDA; IDA; IDA cis-Golgi network; TRAPPIII protein complex; cytosol; TRAPPI protein complex; pre-autophagosomal structure; trans-Golgi network; TRAPPII protein complex GO_0005801; GO_1990072; GO_0005829; GO_1990070; GO_0000407; GO_0005802; GO_1990071 5872 YOR116C RPO31 RNA polymerase III largest subunit C160 RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 C IDA DNA-directed RNA polymerase III complex GO_0005666 5873 YOR117W RPT5 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 C IDA proteasome regulatory particle, base subcomplex GO_0008540 5874 YOR118W RTC5 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity C HDA cytoplasm GO_0005737 5875 YOR119C RIO1 Serine kinase involved in cell cycle regulation and rDNA integrity Serine kinase involved in cell cycling and pre-rRNA processing; associated with late pre-40S particles via its conserved C-terminal domain and participates in late 40S biogenesis; association with pre-40S particles regulated by its catalytic ATPase site and likely occurs after the release of Rio2p from these particles; involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA; essential gene C IDA; IDA; IDA nucleus; nucleolar chromatin; cytoplasm GO_0005634; GO_0030874; GO_0005737 5876 YOR120W GCY1 Glycerol dehydrogenase Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5877 YOR121C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W 5878 YOR122C PFY1 Profilin Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS C IDA; IDA cytosol; extrinsic component of plasma membrane GO_0005829; GO_0019897 5879 YOR123C LEO1 Component of the Paf1 complex Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay C IPI; IDA Cdc73/Paf1 complex; nucleus GO_0016593; GO_0005634 5880 YOR124C UBP2 Ubiquitin-specific protease Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity 5881 YOR125C CAT5 Protein required for ubiquinone (Coenzyme Q) biosynthesis Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5882 YOR126C IAH1 Isoamyl acetate-hydrolyzing esterase Isoamyl acetate-hydrolyzing esterase; required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing 5883 YOR127W RGA1 GTPase-activating protein for polarity-establishment protein Cdc42p GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication C HDA; IDA; HDA cellular bud neck; cellular bud neck split septin rings; cytoplasm GO_0005935; GO_0032177; GO_0005737 5884 YOR128C ADE2 Phosphoribosylaminoimidazole carboxylase Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine C HDA cytoplasm GO_0005737 5885 YOR129C AFI1 Arf3p polarization-specific docking factor Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p C HDA; IMP; IDA; IDA cytoplasm; nucleus; plasma membrane; cellular bud neck GO_0005737; GO_0005634; GO_0005886; GO_0005935 5886 YOR130C ORT1 Ornithine transporter of the mitochondrial inner membrane Ornithine transporter of the mitochondrial inner membrane; exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome C IDA; HDA mitochondrial envelope; mitochondrion GO_0005740; GO_0005739 5887 YOR131C YOR131C Putative haloacid dehalogenase-like hydrolase Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5888 YOR132W VPS17 Subunit of the membrane-associated retromer complex Subunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes C IPI; IMP; IPI endosome; retromer complex; retromer, tubulation complex GO_0005768; GO_0030904; GO_0030905 5889 YOR133W EFT1 Elongation factor 2 (EF-2), also encoded by EFT2 Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication C HDA; IDA cytosol; ribosome GO_0005829; GO_0005840 5890 YOR134W BAG7 Rho GTPase activating protein (RhoGAP) Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication 5891 YOR135C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci 5892 YOR136W IDH2 Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated C IDA; HDA mitochondrial isocitrate dehydrogenase complex (NAD+); mitochondrion GO_0005962; GO_0005739 5893 YOR137C SIA1 Protein of unassigned function Protein of unassigned function; involved in activation of the Pma1p plasma membrane H+-ATPase by glucose; contains peptide signal for membrane localization 5894 YOR138C RUP1 Protein that regulates ubiquitination of Rsp5p Protein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5895 YOR139C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W 5896 YOR140W SFL1 Transcriptional repressor and activator Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C) C IDA nucleus GO_0005634 5897 YOR141C ARP8 Nuclear actin-related protein involved in chromatin remodeling Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; has mRNA binding activity C IDA; IPI nucleus; Ino80 complex GO_0005634; GO_0031011 5898 YOR142W LSC1 Alpha subunit of succinyl-CoA ligase Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 5899 YOR142W-A YOR142W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 5900 YOR142W-B YOR142W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 5901 YOR143C THI80 Thiamine pyrophosphokinase Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) C HDA cytoplasm GO_0005737 5902 YOR144C ELG1 Subunit of an alternative replication factor C complex Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication C HDA; HDA; HDA; IPI cytoplasm; mitochondrion; nucleus; Elg1 RFC-like complex GO_0005737; GO_0005739; GO_0005634; GO_0031391 5903 YOR145C PNO1 Essential nucleolar protein required for pre-18S rRNA processing Essential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain C HDA; IDA; IDA 90S preribosome; nucleus; nucleolus GO_0030686; GO_0005634; GO_0005730 5904 YOR146W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C 5905 YOR147W MDM32 Mitochondrial inner membrane protein with similarity to Mdm31p Mitochondrial inner membrane protein with similarity to Mdm31p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; variation between SK1 and S288C at residues 182 and 262 impacts invasive growth and mitochondrial network structure C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5906 YOR148C SPP2 Essential protein that promotes the first step of splicing Essential protein that promotes the first step of splicing; required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction C IDA spliceosomal complex GO_0005681 5907 YOR149C SMP3 Alpha 1,2-mannosyltransferase Alpha 1,2-mannosyltransferase; involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure C HDA endoplasmic reticulum GO_0005783 5908 YOR150W MRPL23 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2 C HDA; IDA; IDA mitochondrion; mitochondrial large ribosomal subunit; fungal-type vacuole GO_0005739; GO_0005762; GO_0000324 5909 YOR151C RPB2 RNA polymerase II second largest subunit B150 RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit C IDA; HDA; IDA; HDA DNA-directed RNA polymerase II, core complex; nucleus; cytoplasmic stress granule; mitochondrion GO_0005665; GO_0005634; GO_0010494; GO_0005739 5910 YOR152C ATG40 Autophagy receptor with a role in endoplasmic reticulum degradation Putative protein of unknown function; YOR152C is not an essential gene C IDA; IDA membrane; cortical endoplasmic reticulum GO_0016020; GO_0032541 5911 YOR153W PDR5 Plasma membrane ATP-binding cassette (ABC) transporter Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication C HDA; HDA; IDA mitochondrion; cell periphery; plasma membrane GO_0005739; GO_0071944; GO_0005886 5912 YOR154W SLP1 Glycosylated integral ER membrane protein of unknown function Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p C HDA; IDA cell periphery; integral component of endoplasmic reticulum membrane GO_0071944; GO_0030176 5913 YOR155C ISN1 Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase; catalyzes the breakdown of IMP to inosine; responsible for production of nicotinamide riboside and nicotinic acid riboside; expression positively regulated by nicotinic acid and glucose availability; does not show similarity to known 5'-nucleotidases from other organisms 5914 YOR156C NFI1 SUMO E3 ligase SUMO E3 ligase; catalyzes sumoylation of Yku70p/Yku80p and Sir4p promoting chromatin anchoring; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length; NFI1 has a paralog, SIZ1, that arose from the whole genome duplication C HDA; HDA; IDA nucleus; cytoplasm; nuclear chromatin GO_0005634; GO_0005737; GO_0000790 5915 YOR157C PUP1 Beta 2 subunit of the 20S proteasome Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z C IDA; IDA; IDA; HDA nucleus; proteasome storage granule; proteasome core complex, beta-subunit complex; cytosol GO_0005634; GO_0034515; GO_0019774; GO_0005829 5916 YOR158W PET123 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator C IDA; HDA mitochondrial small ribosomal subunit; mitochondrion GO_0005763; GO_0005739 5917 YOR159C SME1 Core Sm protein Sm E Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E C IDA; IDA; IDA; IDA U4/U6 x U5 tri-snRNP complex; U1 snRNP; U5 snRNP; U2-type prespliceosome GO_0046540; GO_0005685; GO_0005682; GO_0071004 5918 YOR160W MTR10 Nuclear import receptor Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress C HDA; HDA; IDA cytoplasm; nuclear periphery; nucleus GO_0005737; GO_0034399; GO_0005634 5919 YOR161C PNS1 Protein of unknown function Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport C HDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 5920 YOR161C-C YOR161C-C Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5921 YOR161W-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5922 YOR161W-B Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5923 YOR162C YRR1 Zn2-Cys6 zinc-finger transcription factor Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 5924 YOR163W DDP1 Polyphosphate phosphatase Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5925 YOR164C GET4 Protein involved in inserting tail-anchored proteins into ER membranes Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 C HDA; IDA cytoplasm; TRC complex GO_0005737; GO_0072380 5926 YOR165W SEY1 Dynamin-like GTPase that mediates homotypic ER fusion Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 C HDA; IDA; HDA; IDA; HDA cell periphery; endoplasmic reticulum; cellular bud; cortical endoplasmic reticulum; cytoplasm GO_0071944; GO_0005783; GO_0005933; GO_0032541; GO_0005737 5927 YOR166C SWT1 RNA endoribonuclease involved in perinuclear mRNP quality control RNA endoribonuclease involved in perinuclear mRNP quality control; involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 5928 YOR167C RPS28A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in regulation of RPS28B, in which Rps28Ap binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28A has a paralog, RPS28B, that arose from the whole genome duplication C IDA cytosolic small ribosomal subunit GO_0022627 5929 YOR168W GLN4 Glutamine tRNA synthetase Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions C IDA; IDA cytosol; mitochondrion GO_0005829; GO_0005739 5930 YOR169C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W 5931 YOR170W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 5932 YOR171C LCB4 Sphingoid long-chain base kinase Sphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; HDA Golgi apparatus; plasma membrane; cortical endoplasmic reticulum; endoplasmic reticulum; cell periphery GO_0005794; GO_0005886; GO_0032541; GO_0005783; GO_0071944 5933 YOR172W YRM1 Zinc finger transcription factor involved in multidrug resistance Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 5934 YOR173W DCS2 m(7)GpppX pyrophosphatase regulator m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication C IDA; HDA P-body; cytoplasm GO_0000932; GO_0005737 5935 YOR174W MED4 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C IDA core mediator complex GO_0070847 5936 YOR175C ALE1 Broad-specificity lysophospholipid acyltransferase Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids C HDA; HDA ribosome; endoplasmic reticulum GO_0005840; GO_0005783 5937 YOR176W HEM15 Ferrochelatase Ferrochelatase; a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 5938 YOR177C MPC54 Component of the meiotic outer plaque Component of the meiotic outer plaque; a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate C HDA; IDA spindle pole body; meiotic spindle pole body GO_0005816; GO_0035974 5939 YOR178C GAC1 Regulatory subunit for Glc7p type-1 protein phosphatase (PP1) Regulatory subunit for Glc7p type-1 protein phosphatase (PP1); tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; GAC1 has a paralog, PIG1, that arose from the whole genome duplication C IDA protein phosphatase type 1 complex GO_0000164 5940 YOR179C SYC1 Subunit of the APT subcomplex of cleavage and polyadenylation factor Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication C IDA; HDA mRNA cleavage and polyadenylation specificity factor complex; nucleus GO_0005847; GO_0005634 5941 YOR180C DCI1 Peroxisomal protein Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed; DCI1 has a paralog, ECI1, that arose from the whole genome duplication C HDA; IDA cytoplasm; peroxisomal matrix GO_0005737; GO_0005782 5942 YOR181W LAS17 Actin assembly factor Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments and a polyproline domain which can nucleate actin filaments independent of Arp2/3; mutants are defective in actin cytoskeleton dependent processes such as: endocytosis, bud site selection and cytokinesis; localizes with the Arp2/3 complex to actin cortical patches; homolog of the Wiskott-Aldrich Syndrome protein (WASP), implicated in severe immunodeficiency C IDA; HDA; HDA actin cortical patch; mating projection tip; cytoplasm GO_0030479; GO_0043332; GO_0005737 5943 YOR182C RPS30B Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30B has a paralog, RPS30A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress 5944 YOR183W FYV12 Protein of unknown function Protein of unknown function; required for survival upon exposure to K1 killer toxin 5945 YOR184W SER1 3-phosphoserine aminotransferase 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 5946 YOR185C GSP2 GTP binding protein (mammalian Ranp homolog) GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication C IGI nucleus GO_0005634 5947 YOR186C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5948 YOR186W YOR186W Putative protein of unknown function Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication 5949 YOR187W TUF1 Mitochondrial translation elongation factor Tu (EF-Tu) Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans C HDA mitochondrion GO_0005739 5950 YOR188W MSB1 Protein of unknown function Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA; HDA; HDA; HDA cellular bud neck; mitochondrion; cellular bud tip; plasma membrane; cytoplasm GO_0005935; GO_0005739; GO_0005934; GO_0005886; GO_0005737 5951 YOR189W IES4 Component of the INO80 chromatiin remodeling complex Component of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses C HDA; IDA nucleus; Ino80 complex GO_0005634; GO_0031011 5952 YOR190W SPR1 Sporulation-specific exo-1,3-beta-glucanase Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance; SPR1 has a paralog, EXG1, that arose from the whole genome duplication C IDA; HDA; HDA; IMP ascospore wall; prospore membrane; fungal-type vacuole; fungal-type cell wall GO_0005619; GO_0005628; GO_0000324; GO_0009277 5953 YOR191W ULS1 Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL) Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress C HDA; IDA; IDA; IDA; HDA mitochondrion; nucleus; nucleolus; condensed nuclear chromosome kinetochore; cytoplasm GO_0005739; GO_0005634; GO_0005730; GO_0000778; GO_0005737 5954 YOR192C THI72 Transporter of thiamine or related compound Transporter of thiamine or related compound; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); shares sequence similarity with Thi7p C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 5955 YOR192C-A YOR192C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 5956 YOR192C-B YOR192C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 5957 YOR192C-C YOR192C-C Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 5958 YOR193W PEX27 Peripheral peroxisomal membrane protein Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication C IDA peroxisomal membrane GO_0005778 5959 YOR194C TOA1 TFIIA large subunit TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa2p, for ribosomal protein gene transcription in vivo; homologous to largest and second largest subunits of human and Drosophila TFIIA C IDA transcription factor TFIIA complex GO_0005672 5960 YOR195W SLK19 Kinetochore-associated protein Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate C IDA; IDA; IDA; IDA kinetochore; spindle; condensed nuclear chromosome kinetochore; spindle midzone GO_0000776; GO_0005819; GO_0000778; GO_0051233 5961 YOR196C LIP5 Protein involved in biosynthesis of the coenzyme lipoic acid Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase C HDA mitochondrion GO_0005739 5962 YOR197W MCA1 Ca2+-dependent cysteine protease Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 5963 YOR198C BFR1 Component of mRNP complexes associated with polyribosomes Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity C IDA; IDA; IDA; IDA; IDA ribonucleoprotein complex; endoplasmic reticulum; nuclear outer membrane-endoplasmic reticulum membrane network; polysome; cytoplasm GO_1990904; GO_0005783; GO_0042175; GO_0005844; GO_0005737 5964 YOR199W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5965 YOR200W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c 5966 YOR201C MRM1 Ribose methyltransferase Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA C IDA mitochondrion GO_0005739 5967 YOR202W HIS3 Imidazoleglycerol-phosphate dehydratase Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p 5968 YOR203W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation 5969 YOR204W DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase; required for translation initiation of all yeast mRNAs; binds to mRNA cap-associated factors, and binding stimulates Ded1p RNA-dependent ATPase activity; mutation in human homolog DBY is associated with male infertility; human homolog DDX3X complements ded1 null mutation; DED1 has a paralog, DBP1, that arose from the whole genome duplication C IDA; IDA; IDA nucleus; cytoplasmic stress granule; cytoplasm GO_0005634; GO_0010494; GO_0005737 5970 YOR205C GEP3 Protein required for mitochondrial ribosome small subunit biogenesis Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p) C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5971 YOR206W NOC2 Protein involved in ribosome biogenesis Protein involved in ribosome biogenesis; forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes; forms a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit C IDA; IDA; IPI; HDA; IPI nucleus; preribosome, large subunit precursor; Noc1p-Noc2p complex; mitochondrion; Noc2p-Noc3p complex GO_0005634; GO_0030687; GO_0030690; GO_0005739; GO_0030691 5972 YOR207C RET1 Second-largest subunit of RNA polymerase III Second-largest subunit of RNA polymerase III; RNA polymerase III is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs C IDA DNA-directed RNA polymerase III complex GO_0005666 5973 YOR208W PTP2 Nuclear phosphotyrosine-specific phosphatase involved in osmosensing Nuclear phosphotyrosine-specific phosphatase involved in osmosensing; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; with Msg5p co-regulates the calcium signaling pathway C IDA nucleus GO_0005634 5974 YOR209C NPT1 Nicotinate phosphoribosyltransferase Nicotinate phosphoribosyltransferase; acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus C IDA nucleus GO_0005634 5975 YOR210W RPB10 RNA polymerase subunit ABC10-beta RNA polymerase subunit ABC10-beta; common to RNA polymerases I, II, and III C IDA; IDA; IDA DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase III complex GO_0005736; GO_0005665; GO_0005666 5976 YOR211C MGM1 Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy C IDA; IDA; HDA; IDA; IDA; IDA intrinsic component of mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; mitochondrial crista; extrinsic component of mitochondrial inner membrane; mitochondrial inner boundary membrane GO_0031304; GO_0005758; GO_0005739; GO_0030061; GO_0031314; GO_0097002 5977 YOR212W STE4 G protein beta subunit G protein beta subunit; forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats C IDA; IMP; IDA plasma membrane; heterotrimeric G-protein complex; mating projection GO_0005886; GO_0005834; GO_0005937 5978 YOR213C SAS5 Subunit of the SAS complex (Sas2p, Sas4p, Sas5p) Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity C IDA; IPI nuclear chromatin; SAS acetyltransferase complex GO_0000790; GO_0033255 5979 YOR214C SPR2 Putative spore wall protein Putative spore wall protein; expression increases during sporulation; not an essential gene; YOR214C has a paralog, SPO19, that arose from the whole genome duplication C HDA; IDA fungal-type vacuole; fungal-type cell wall GO_0000324; GO_0009277 5980 YOR215C AIM41 Protein of unknown function Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 5981 YOR216C RUD3 Golgi matrix protein Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 C IDA; HDA Golgi membrane; Golgi apparatus GO_0000139; GO_0005794 5982 YOR217W RFC1 Subunit of heteropentameric Replication factor C (RF-C) Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon C IDA DNA replication factor C complex GO_0005663 5983 YOR218C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W 5984 YOR219C STE13 Dipeptidyl aminopeptidase Dipeptidyl aminopeptidase; Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor C IDA trans-Golgi network GO_0005802 5985 YOR220W RCN2 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 5986 YOR221C MCT1 Predicted malonyl-CoA:ACP transferase Predicted malonyl-CoA:ACP transferase; putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling C IMP mitochondrion GO_0005739 5987 YOR222W ODC2 Mitochondrial inner membrane transporter Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism; ODC2 has a paralog, ODC1, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 5988 YOR223W DSC3 Subunit of the DSC ubiquitin ligase complex Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 C IDA; IDA; IDA fungal-type vacuole lumen; Dsc E3 ubiquitin ligase complex; endoplasmic reticulum GO_0000328; GO_0044695; GO_0005783 5989 YOR224C RPB8 RNA polymerase subunit ABC14.5 RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III C IDA; IDA; IDA DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase III complex GO_0005665; GO_0005736; GO_0005666 5990 YOR225W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 5991 YOR226C ISU2 Mitochondrial protein required for iron-sulfur protein synthesis Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved; ISU2 has a paralog, ISU1, that arose from the whole genome duplication C IDA mitochondrial matrix GO_0005759 5992 YOR227W HER1 Protein of unknown function Protein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication C HDA; HDA; HDA cytoplasm; ribosome; mitochondrion GO_0005737; GO_0005840; GO_0005739 5993 YOR228C MCP1 Mitochondrial protein of unknown function involved in lipid homeostas Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains C IDA; HDA; IDA mitochondrial outer membrane; mitochondrion; integral component of mitochondrial membrane GO_0005741; GO_0005739; GO_0032592 5994 YOR229W WTM2 Transcriptional modulator Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 5995 YOR230W WTM1 Transcriptional modulator Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats C HDA nucleus GO_0005634 5996 YOR231C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5997 YOR231W MKK1 MAPKK involved in the protein kinase C signaling pathway MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA cellular bud tip; cellular bud neck; mating projection tip; cytoplasm GO_0005934; GO_0005935; GO_0043332; GO_0005737 5998 YOR232W MGE1 Mitochondrial matrix cochaperone Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress C HDA; IDA; IDA mitochondrion; presequence translocase-associated import motor; mitochondrial matrix GO_0005739; GO_0001405; GO_0005759 5999 YOR233W KIN4 Serine/threonine protein kinase Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication C IDA; IDA; IDA cell cortex; spindle pole body; cellular bud neck GO_0005938; GO_0005816; GO_0005935 6000 YOR234C RPL33B Ribosomal 60S subunit protein L33B Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6001 YOR235W IRC13 Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci 6002 YOR236W DFR1 Dihydrofolate reductase involved in tetrahydrofolate biosynthesis Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 6003 YOR237W HES1 Protein implicated in the regulation of ergosterol biosynthesis Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole membrane; cellular bud neck GO_0000329; GO_0005935 6004 YOR238W YOR238W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C HDA cytoplasm GO_0005737 6005 YOR239W ABP140 AdoMet-dependent tRNA methyltransferase and actin binding protein AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift C IDA; HDA; IDA; IDA actin cortical patch; mating projection tip; actin filament bundle; actin filament GO_0030479; GO_0043332; GO_0032432; GO_0005884 6006 YOR241W MET7 Folylpolyglutamate synthetase Folylpolyglutamate synthetase; catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA cytoplasm; mitochondrion; endoplasmic reticulum GO_0005737; GO_0005739; GO_0005783 6007 YOR242C SSP2 Sporulation specific protein that localizes to the spore wall Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis C HDA; IDA; HDA nucleus; ascospore wall; prospore membrane GO_0005634; GO_0005619; GO_0005628 6008 YOR243C PUS7 Pseudouridine synthase Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 6009 YOR244W ESA1 Catalytic subunit of the histone acetyltransferase complex (NuA4) Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases C IDA; IDA; IDA; IDA Piccolo NuA4 histone acetyltransferase complex; NuA4 histone acetyltransferase complex; nuclear chromatin; nuclear telomeric heterochromatin GO_0032777; GO_0035267; GO_0000790; GO_0005724 6010 YOR245C DGA1 Diacylglycerol acyltransferase Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles C IDA; HDA; IDA lipid particle; endoplasmic reticulum; integral component of membrane GO_0005811; GO_0005783; GO_0016021 6011 YOR246C ENV9 Protein proposed to be involved in vacuolar functions Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts C HDA; IDA lipid particle; mitochondrion GO_0005811; GO_0005739 6012 YOR247W SRL1 Mannoprotein that exhibits a tight association with the cell wall Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication C HDA; HDA; IDA; HDA; IDA fungal-type vacuole; cell periphery; fungal-type cell wall; cytoplasm; cellular bud tip GO_0000324; GO_0071944; GO_0009277; GO_0005737; GO_0005934 6013 YOR248W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6014 YOR249C APC5 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress C IDA; HDA anaphase-promoting complex; nucleus GO_0005680; GO_0005634 6015 YOR250C CLP1 Component of the cleavage and polyadenylation factor I (CF I) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation and in gene looping which affects reinitiation of transcription C IPI mRNA cleavage factor complex GO_0005849 6016 YOR251C TUM1 Rhodanese domain sulfur transferase Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized C HDA; HDA cytoplasm; mitochondrion GO_0005737; GO_0005739 6017 YOR252W TMA16 Protein of unknown function that associates with ribosomes Protein of unknown function that associates with ribosomes C IDA; HDA ribosome; nucleus GO_0005840; GO_0005634 6018 YOR253W NAT5 Subunit of protein N-terminal acetyltransferase NatA Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing C IDA; IDA cytosolic ribosome; NatA complex GO_0022626; GO_0031415 6019 YOR254C SEC63 Essential subunit of Sec63 complex Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, Sec66p and Sec72p C IPI; HDA; HDA Sec62/Sec63 complex; endoplasmic reticulum; mitochondrion GO_0031207; GO_0005783; GO_0005739 6020 YOR255W OSW1 Protein involved in sporulation Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p C IDA prospore membrane GO_0005628 6021 YOR256C TRE2 Transferrin receptor-like protein Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication 6022 YOR257W CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation C IPI; IDA; IDA nucleotide-excision repair factor 2 complex; transcription export complex 2; half bridge of spindle pole body GO_0000111; GO_0070390; GO_0005825 6023 YOR258W HNT3 DNA 5' AMP hydrolase involved in DNA repair DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6024 YOR259C RPT4 ATPase of the 19S regulatory particle of the 26S proteasome ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization C IDA; IDA proteasome regulatory particle, base subcomplex; nucleus GO_0008540; GO_0005634 6025 YOR260W GCD1 Gamma subunit of the translation initiation factor eIF2B Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression C IDA; IDA; HDA eukaryotic translation initiation factor 2B complex; guanyl-nucleotide exchange factor complex; cytosol GO_0005851; GO_0032045; GO_0005829 6026 YOR261C RPN8 Essential non-ATPase regulatory subunit of the 26S proteasome Essential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p C IDA; IDA proteasome storage granule; proteasome regulatory particle, lid subcomplex GO_0034515; GO_0008541 6027 YOR262W GPN2 Putative GTPase with a role in biogenesis of RNA pol II and polIII Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion C HDA cytoplasm GO_0005737 6028 YOR263C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W 6029 YOR264W DSE3 Daughter cell-specific protein, may help establish daughter fate Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA; HDA cytoplasm; cellular bud neck; nucleus GO_0005737; GO_0005935; GO_0005634 6030 YOR265W RBL2 Protein involved in microtubule morphogenesis Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 6031 YOR266W PNT1 Mitochondrial integral inner membrane protein Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine C IDA; HDA integral component of mitochondrial inner membrane; mitochondrion GO_0031305; GO_0005739 6032 YOR267C HRK1 Protein kinase Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress C HDA cytoplasm GO_0005737 6033 YOR268C YOR268C Putative protein of unknown function Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene 6034 YOR269W PAC1 Involved in nuclear migration, part of the dynein/dynactin pathway Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly C IDA; IDA; HDA cytoplasmic microtubule; nucleus; cytoplasm GO_0005881; GO_0005634; GO_0005737 6035 YOR270C VPH1 Subunit a of vacuolar-ATPase V0 domain Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress C IPI; IDA; IDA vacuolar proton-transporting V-type ATPase, V0 domain; fungal-type vacuole membrane; fungal-type vacuole GO_0000220; GO_0000329; GO_0000324 6036 YOR271C FSF1 Putative protein Putative protein; predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis C HDA mitochondrion GO_0005739 6037 YOR272W YTM1 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats C IPI; IDA; IDA; HDA PeBoW complex; preribosome, large subunit precursor; nucleolus; nucleus GO_0070545; GO_0030687; GO_0005730; GO_0005634 6038 YOR273C TPO4 Polyamine transporter of the major facilitator superfamily Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane C IDA; IMP; HDA plasma membrane; fungal-type vacuole membrane; cell periphery GO_0005886; GO_0000329; GO_0071944 6039 YOR274W MOD5 Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects C HDA; IDA; IDA; IDA mitochondrion; cytosol; nucleolus; nucleus GO_0005739; GO_0005829; GO_0005730; GO_0005634 6040 YOR275C RIM20 Protein involved in proteolytic activation of Rim101p Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation C IDA; HDA; HDA plasma membrane; cytoplasm; nucleus GO_0005886; GO_0005737; GO_0005634 6041 YOR276W CAF20 Phosphoprotein of the mRNA cap-binding complex Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translation initiation; competes with eIF4G for binding to eIF4E C HDA; IPI cytoplasm; mRNA cap binding complex GO_0005737; GO_0005845 6042 YOR277C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 6043 YOR278W HEM4 Uroporphyrinogen III synthase Uroporphyrinogen III synthase; catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria 6044 YOR279C RFM1 Component of the Sum1p-Rfm1p-Hst1p complex Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication C IPI; IPI nucleus; histone deacetylase complex GO_0005634; GO_0000118 6045 YOR280C FSH3 Putative serine hydrolase Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 6046 YOR281C PLP2 Protein that interacts with the CCT complex to stimulate actin folding Protein that interacts with the CCT complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP; CCT is short for chaperonin containing TCP-1; essential gene C IPI cytoplasm GO_0005737 6047 YOR282W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C 6048 YOR283W YOR283W Phosphatase with a broad substrate specificity Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6049 YOR284W HUA2 Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly C HDA cytoplasm GO_0005737 6050 YOR285W RDL1 Thiosulfate sulfurtransferase Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene C HDA; HDA; HDA endoplasmic reticulum; mitochondrion; mitochondrial outer membrane GO_0005783; GO_0005739; GO_0005741 6051 YOR286W RDL2 Protein with rhodanese activity Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss C HDA mitochondrion GO_0005739 6052 YOR287C RRP36 Component of 90S preribosomes Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit C IDA; IDA nucleolus; 90S preribosome GO_0005730; GO_0030686 6053 YOR288C MPD1 Member of the protein disulfide isomerase (PDI) family Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation C HDA; HDA fungal-type vacuole; endoplasmic reticulum GO_0000324; GO_0005783 6054 YOR289W YOR289W Putative protein of unknown function Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6055 YOR290C SNF2 Catalytic subunit of the SWI/SNF chromatin remodeling complex Catalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p C IDA; IDA nucleus; SWI/SNF complex GO_0005634; GO_0016514 6056 YOR291W YPK9 Vacuolar protein with a possible role in sequestering heavy metals Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome C IDA; IDA vacuole-mitochondrion membrane contact site; fungal-type vacuole membrane GO_1990816; GO_0000329 6057 YOR292C YOR292C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene C HDA fungal-type vacuole GO_0000324 6058 YOR293C-A YOR293C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 6059 YOR293W RPS10A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication 6060 YOR294W RRS1 Essential protein that binds ribosomal protein L11 Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice C IDA; IDA; IDA; IDA nucleolus; preribosome, large subunit precursor; nuclear periphery; nucleoplasm GO_0005730; GO_0030687; GO_0034399; GO_0005654 6061 YOR295W UAF30 Subunit of UAF (upstream activation factor) complex Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication C IDA; IDA nucleolus; RNA polymerase I upstream activating factor complex GO_0005730; GO_0000500 6062 YOR296W YOR296W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene C HDA cytoplasm GO_0005737 6063 YOR297C TIM18 Component of the mitochondrial TIM22 complex Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex C IDA; HDA mitochondrial inner membrane protein insertion complex; mitochondrion GO_0042721; GO_0005739 6064 YOR298C-A MBF1 Transcriptional coactivator Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress C HDA mitochondrion GO_0005739 6065 YOR298W MUM3 Protein of unknown function involved in outer spore wall organization Protein of unknown function involved in outer spore wall organization; has similarity to the tafazzins superfamily of acyltransferases 6066 YOR299W BUD7 Member of the ChAPs family (Chs5p-Arf1p-binding proteins) Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication C HDA; IDA; IDA clathrin-coated vesicle; exomer complex; trans-Golgi network transport vesicle GO_0030136; GO_0034044; GO_0030140 6067 YOR300W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 6068 YOR301W RAX1 Protein involved in bud site selection during bipolar budding Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily C IDA; HDA cellular bud neck; fungal-type vacuole GO_0005935; GO_0000324 6069 YOR302W YOR302W CPA1 uORF CPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA 6070 YOR303W CPA1 Small subunit of carbamoyl phosphate synthetase Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader C HDA; IDA; IDA cytoplasm; cytoplasmic stress granule; carbamoyl-phosphate synthase complex GO_0005737; GO_0010494; GO_0005951 6071 YOR304C-A BIL1 Protein that binds Bud6p and has a role in actin cable assembly Protein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck C HDA; IDA; HDA; HDA; HDA cellular bud neck; cellular bud tip; cytoplasm; cellular bud; mating projection tip GO_0005935; GO_0005934; GO_0005737; GO_0005933; GO_0043332 6072 YOR304W ISW2 ATP-dependent DNA translocase involved in chromatin remodeling ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide C HDA; IPI nucleus; CHRAC GO_0005634; GO_0008623 6073 YOR305W RRG7 Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene C HDA mitochondrion GO_0005739 6074 YOR306C MCH5 Plasma membrane riboflavin transporter Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport C IDA plasma membrane GO_0005886 6075 YOR307C SLY41 Protein involved in ER-to-Golgi transport Protein involved in ER-to-Golgi transport C HDA endoplasmic reticulum GO_0005783 6076 YOR308C SNU66 Component of the U4/U6.U5 snRNP complex Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 C IDA U4/U6 x U5 tri-snRNP complex GO_0046540 6077 YOR309C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 6078 YOR310C NOP58 Protein involved in producing mature rRNAs and snoRNAs Protein involved in producing mature rRNAs and snoRNAs; involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA C IPI; IDA; HDA; IDA box C/D snoRNP complex; nucleolus; 90S preribosome; small-subunit processome GO_0031428; GO_0005730; GO_0030686; GO_0032040 6079 YOR311C DGK1 Diacylglycerol kinase Diacylglycerol kinase; localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain C HDA; IDA endoplasmic reticulum; integral component of endoplasmic reticulum membrane GO_0005783; GO_0030176 6080 YOR312C RPL20B Ribosomal 60S subunit protein L20B Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6081 YOR313C SPS4 Protein whose expression is induced during sporulation Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage C HDA lipid particle GO_0005811 6082 YOR314W YOR314W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6083 YOR314W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6084 YOR315W SFG1 Nuclear protein putative transcription factor Nuclear protein putative transcription factor; required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6085 YOR316C COT1 Vacuolar transporter that mediates zinc transport into the vacuole Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication C IDA; HDA; HDA fungal-type vacuole; mitochondrion; fungal-type vacuole membrane GO_0000324; GO_0005739; GO_0000329 6086 YOR316C-A YOR316C-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 6087 YOR317W FAA1 Long chain fatty acyl-CoA synthetase Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication C HDA; HDA; HDA; HDA; IDA plasma membrane; mitochondrial outer membrane; endoplasmic reticulum; mitochondrion; lipid particle GO_0005886; GO_0005741; GO_0005783; GO_0005739; GO_0005811 6088 YOR318C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo 6089 YOR319W HSH49 U2-snRNP associated splicing factor U2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) C IDA; IDA U2-type prespliceosome; U2 snRNP GO_0071004; GO_0005686 6090 YOR320C GNT1 N-acetylglucosaminyltransferase N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus C IDA Golgi medial cisterna GO_0005797 6091 YOR321W PMT3 Protein O-mannosyltransferase Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication C HDA; IDA; IDA endoplasmic reticulum; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex; dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex GO_0005783; GO_0097583; GO_0097585 6092 YOR322C LDB19 Alpha-arrestin involved in ubiquitin-dependent endocytosis Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane C HDA; IDA; IDA; HDA; HDA cytosol; plasma membrane; Golgi apparatus; cytoplasm; clathrin-coated vesicle GO_0005829; GO_0005886; GO_0005794; GO_0005737; GO_0030136 6093 YOR323C PRO2 Gamma-glutamyl phosphate reductase Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6094 YOR324C FRT1 Tail-anchored ER membrane protein of unknown function Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication C IDA endoplasmic reticulum GO_0005783 6095 YOR325W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 6096 YOR326W MYO2 Type V myosin motor involved in actin-based transport of cargos Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication C IPI; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IMP Myo2p-Vac17p-Vac8p transport complex; incipient cellular bud site; cellular bud neck; transport vesicle; fungal-type vacuole membrane; vesicle; mating projection tip; filamentous actin; cellular bud tip; actin filament bundle GO_0071563; GO_0000131; GO_0005935; GO_0030133; GO_0000329; GO_0031982; GO_0043332; GO_0031941; GO_0005934; GO_0032432 6097 YOR327C SNC2 Vesicle membrane receptor protein (v-SNARE) Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; Snc2p levels regulated by Vps45p; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication C IDA; HDA; HDA; IDA; IPI; IDA endosome; cell periphery; cellular bud; trans-Golgi network; SNARE complex; transport vesicle membrane GO_0005768; GO_0071944; GO_0005933; GO_0005802; GO_0031201; GO_0030658 6098 YOR328W PDR10 ATP-binding cassette (ABC) transporter ATP-binding cassette (ABC) transporter; multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p C HDA; IDA; HDA fungal-type vacuole; plasma membrane; cell periphery GO_0000324; GO_0005886; GO_0071944 6099 YOR329C SCD5 Protein required for normal actin organization and endocytosis Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency C IMP; IDA nucleus; actin cortical patch GO_0005634; GO_0030479 6100 YOR329W-A Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR 6101 YOR330C MIP1 Mitochondrial DNA polymerase gamma Mitochondrial DNA polymerase gamma subunit; conserved C-terminal segment is required for the maintenance of mitochondrial genome; its human ortholog, POLG, can rescue mip1 phenotypes, and mutations in POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit C HDA mitochondrion GO_0005739 6102 YOR331C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W 6103 YOR332W VMA4 Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase) Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress C IDA; IDA fungal-type vacuole membrane; vacuolar proton-transporting V-type ATPase, V1 domain GO_0000329; GO_0000221 6104 YOR333C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of MRS2 gene required for respiratory growth 6105 YOR334W MRS2 Mitochondrial inner membrane Mg(2+) channel Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 6106 YOR335C ALA1 Cytoplasmic and mitochondrial alanyl-tRNA synthetase Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog C IDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 6107 YOR335W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ALA1/YOR335C 6108 YOR336W KRE5 Protein required for beta-1,6 glucan biosynthesis Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects C HDA endoplasmic reticulum GO_0005783 6109 YOR337W TEA1 Ty1 enhancer activator involved in Ty enhancer-mediated transcription Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein C IPI; IDA nucleus; cell cortex of cell tip GO_0005634; GO_0051285 6110 YOR338W YOR338W Putative protein of unknown function Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication 6111 YOR339C UBC11 Ubiquitin-conjugating enzyme Ubiquitin-conjugating enzyme; most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 6112 YOR340C RPA43 RNA polymerase I subunit A43 RNA polymerase I subunit A43 C IDA DNA-directed RNA polymerase I complex GO_0005736 6113 YOR341W RPA190 RNA polymerase I largest subunit A190 RNA polymerase I largest subunit A190 C IDA; IDA nucleolus; DNA-directed RNA polymerase I complex GO_0005730; GO_0005736 6114 YOR342C YOR342C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6115 YOR343C YOR343C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6116 YOR343W-A YOR343W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 6117 YOR343W-B YOR343W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 6118 YOR344C TYE7 Serine-rich protein that contains a bHLH DNA binding motif Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix C IDA nuclear chromatin GO_0000790 6119 YOR345C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 6120 YOR346W REV1 Deoxycytidyl transferase Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress C IDA; IPI; IDA; HDA; HDA mitochondrion; replication fork; nuclear chromatin; cytoplasm; nucleus GO_0005739; GO_0005657; GO_0000790; GO_0005737; GO_0005634 6121 YOR347C PYK2 Pyruvate kinase Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication C HDA; IDA cytoplasm; mitochondrion GO_0005737; GO_0005739 6122 YOR348C PUT4 Proline permease Proline permease; required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells 6123 YOR349W CIN1 Tubulin folding factor D involved in beta-tubulin (Tub2p) folding Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl 6124 YOR350C MNE1 Protein involved in splicing Group I aI5-beta intron from COX1 mRNA Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein C HDA; IDA; IDA; HDA cytoplasm; ribonucleoprotein complex; mitochondrial matrix; mitochondrion GO_0005737; GO_1990904; GO_0005759; GO_0005739 6125 YOR351C MEK1 Meiosis-specific serine/threonine protein kinase Meiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis C IDA nucleus GO_0005634 6126 YOR352W TFB6 Subunit of TFIIH complex Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus C IDA; HDA; HDA holo TFIIH complex; cytoplasm; nucleus GO_0005675; GO_0005737; GO_0005634 6127 YOR353C SOG2 Key component of the RAM signaling network Key component of the RAM signaling network; required for proper cell morphogenesis and cell separation after mitosis C IDA; IDA; HDA; IDA mating projection tip; incipient cellular bud site; cytoplasm; cellular bud GO_0043332; GO_0000131; GO_0005737; GO_0005933 6128 YOR354C MSC6 Multicopy suppressor of HER2 involved in mitochondrial translation Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C IDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 6129 YOR355W GDS1 Protein of unknown function Protein of unknown function; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA; HDA; HDA nucleus; mitochondrion; cytoplasm GO_0005634; GO_0005739; GO_0005737 6130 YOR356W CIR2 Putative ortholog of human ETF-dH Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase C IDA mitochondrion GO_0005739 6131 YOR357C SNX3 Sorting nexin for late-Golgi enzymes Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p C IDA; HDA; IDA cytosol; fungal-type vacuole membrane; endosome GO_0005829; GO_0000329; GO_0005768 6132 YOR358W HAP5 Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex C IPI CCAAT-binding factor complex GO_0016602 6133 YOR359W VTS1 Flap-structured DNA-binding and RNA-binding protein Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity C IDA; IDA; IDA nucleus; P-body; cytosol GO_0005634; GO_0000932; GO_0005829 6134 YOR360C PDE2 High-affinity cyclic AMP phosphodiesterase High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6135 YOR361C PRT1 eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3) eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes C IPI; IDA; IDA; IDA eukaryotic translation initiation factor 3 complex; cytoplasmic stress granule; multi-eIF complex; eukaryotic 48S preinitiation complex GO_0005852; GO_0010494; GO_0043614; GO_0033290 6136 YOR362C PRE10 Alpha 7 subunit of the 20S proteasome Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress C IDA; IDA proteasome core complex, alpha-subunit complex; proteasome storage granule GO_0019773; GO_0034515 6137 YOR363C PIP2 Autoregulatory, oleate-activated transcription factor Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication C IDA Pip2-Oaf1 complex GO_0089716 6138 YOR364W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C 6139 YOR365C YOR365C Putative protein of unknown function Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication 6140 YOR366W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C 6141 YOR367W SCP1 Component of yeast cortical actin cytoskeleton Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin C IDA actin cortical patch GO_0030479 6142 YOR368W RAD17 Checkpoint protein Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins C IPI; IDA nucleus; checkpoint clamp complex GO_0005634; GO_0030896 6143 YOR369C RPS12 Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog C IDA cytosolic small ribosomal subunit GO_0022627 6144 YOR370C MRS6 Rab escort protein Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress C IDA; IDA; HDA; HDA; IDA Rab-protein geranylgeranyltransferase complex; membrane; cytosol; nucleus; cytoplasm GO_0005968; GO_0016020; GO_0005829; GO_0005634; GO_0005737 6145 YOR371C GPB1 Multistep regulator of cAMP-PKA signaling Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication C HDA; IGI cytoplasm; plasma membrane GO_0005737; GO_0005886 6146 YOR372C NDD1 Transcriptional activator essential for nuclear division Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes C IDA nucleus GO_0005634 6147 YOR373W NUD1 Component of the spindle pole body outer plaque Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance C IDA spindle pole body GO_0005816 6148 YOR374W ALD4 Mitochondrial aldehyde dehydrogenase Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol C HDA; IDA mitochondrion; mitochondrial nucleoid GO_0005739; GO_0042645 6149 YOR375C GDH1 NADP(+)-dependent glutamate dehydrogenase NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6150 YOR376W YOR376W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene 6151 YOR376W-A YOR376W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 6152 YOR377W ATF1 Alcohol acetyltransferase Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism C IDA; HDA lipid particle; endoplasmic reticulum GO_0005811; GO_0005783 6153 YOR378W AMF1 Low affinity NH4+ transporter Putative paralog of ATR1; but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 6154 YOR379C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W 6155 YOR380W RDR1 Transcriptional repressor involved in regulating multidrug resistance Transcriptional repressor involved in regulating multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins 6156 YOR381W FRE3 Ferric reductase Ferric reductase; reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels C HDA; IDA fungal-type vacuole; plasma membrane GO_0000324; GO_0005886 6157 YOR381W-A YOR381W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 6158 YOR382W FIT2 Mannoprotein that is incorporated into the cell wall Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall C HDA; IDA; HDA cell periphery; fungal-type cell wall; fungal-type vacuole GO_0071944; GO_0009277; GO_0000324 6159 YOR383C FIT3 Mannoprotein that is incorporated into the cell wall Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall C IDA; HDA; HDA fungal-type cell wall; cell periphery; fungal-type vacuole GO_0009277; GO_0071944; GO_0000324 6160 YOR384W FRE5 Putative ferric reductase with similarity to Fre2p Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 6161 YOR385W YOR385W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene C HDA cytoplasm GO_0005737 6162 YOR386W PHR1 DNA photolyase involved in photoreactivation DNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p C HDA; HDA; HDA nucleus; cytoplasm; mitochondrion GO_0005634; GO_0005737; GO_0005739 6163 YOR387C YOR387C Putative protein of unknown function Putative protein of unknown function; regulated by the metal-responsive Aft1p transcription factor; highly inducible in zinc-depleted conditions; localizes to the soluble fraction; YOR387C has a paralog, VEL1, that arose from a single-locus duplication 6164 YOR388C FDH1 NAD(+)-dependent formate dehydrogenase NAD(+)-dependent formate dehydrogenase; may protect cells from exogenous formate C IDA cytosol GO_0005829 6165 YOR389W YOR389W Putative protein of unknown function Putative protein of unknown function; expression regulated by copper levels 6166 YOR390W FEX1 Protein involved in fluoride export Protein involved in fluoride export; nearly identical to FEX2, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 6167 YOR391C HSP33 Possible chaperone and cysteine protease Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer 6168 YOR392W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; gene expression induced by heat 6169 YOR393W ERR1 Putative phosphopyruvate hydratase Protein of unknown function; has similarity to enolases 6170 YOR394C-A YOR394C-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6171 YOR394W PAU21 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau22p; encodes 2 proteins that are translated from 2 different start codons C HDA cytosol GO_0005829 6172 YOR396C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6173 YOR396W YRF1-8 One of several telomeric Y' element-encoded DNA helicases One of several telomeric Y' element-encoded DNA helicases; known as Y'-Help1 (Y'-HELicase Protein 1) 6174 YPL001W HAT1 Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex; uses the cofactor acetyl coenzyme A to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair C IDA; IDA; IDA cytoplasm; nucleus; histone acetyltransferase complex GO_0005737; GO_0005634; GO_0000123 6175 YPL002C SNF8 Component of the ESCRT-II complex Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression C IDA ESCRT II complex GO_0000814 6176 YPL003W ULA1 Protein that activates Rub1p (NEDD8) before neddylation Protein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation 6177 YPL004C LSP1 Eisosome core component Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family C HDA; HDA; IDA; HDA; HDA; HDA mitochondrion; plasma membrane; eisosome; cell periphery; cytoplasm; mitochondrial outer membrane GO_0005739; GO_0005886; GO_0032126; GO_0071944; GO_0005737; GO_0005741 6178 YPL005W AEP3 Peripheral mitochondrial inner membrane protein Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA C HDA; IDA; IDA; HDA mitochondrion; extrinsic component of membrane; mitochondrial inner membrane; cytoplasm GO_0005739; GO_0019898; GO_0005743; GO_0005737 6179 YPL006W NCR1 Vacuolar membrane protein Vacuolar membrane protein; transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to the accumulation of high amounts of lipids observed in patients with Neimann-Pick C, a disease caused by loss-of-function mutations in NPC1, the functional ortholog of Ncr1p C HDA; IDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 6180 YPL007C TFC8 Subunit of RNA polymerase III transcription initiation factor complex Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 C IDA transcription factor TFIIIC complex GO_0000127 6181 YPL008W CHL1 Probable DNA helicase Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome C IMP; IDA nuclear chromatin; nucleus GO_0000790; GO_0005634 6182 YPL009C RQC2 Component of RQC, which mediates nascent chain degradation Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation involved in protein translation; monitors translation stress and signals this to Hsf1p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm C IDA; HDA; IDA; HDA RQC complex; cytoplasm; cytoplasmic stress granule; ribosome GO_1990112; GO_0005737; GO_0010494; GO_0005840 6183 YPL010W RET3 Zeta subunit of the coatomer complex (COPI) Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER C IMP COPI vesicle coat GO_0030126 6184 YPL011C TAF3 TFIID subunit (47 kDa) TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation C IDA transcription factor TFIID complex GO_0005669 6185 YPL012W RRP12 Protein required for export of the ribosomal subunits Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; also plays a role in the cell cycle and the DNA damage response; contains HEAT-repeats C HDA; IDA; HDA; HDA nucleolus; nucleus; 90S preribosome; cytoplasm GO_0005730; GO_0005634; GO_0030686; GO_0005737 6186 YPL013C MRPS16 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 6187 YPL014W CIP1 Cyclin-dependent kinase inhibitor Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6188 YPL015C HST2 Cytoplasmic NAD(+)-dependent protein deacetylase Cytoplasmic NAD(+)-dependent protein deacetylase; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export C IDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 6189 YPL016W SWI1 Subunit of the SWI/SNF chromatin remodeling complex Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer C IMP; IDA SWI/SNF complex; nucleus GO_0016514; GO_0005634 6190 YPL017C IRC15 Microtubule associated protein Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication C IDA; HDA microtubule; cytoplasm GO_0005874; GO_0005737 6191 YPL018W CTF19 Outer kinetochore protein, needed for accurate chromosome segregation Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 C IDA; IDA; IDA condensed nuclear chromosome kinetochore; COMA complex; nucleus GO_0000778; GO_0000817; GO_0005634 6192 YPL019C VTC3 Regulatory subunit of the vacuolar transporter chaperone (VTC) complex Subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication C IDA; IDA; IPI fungal-type vacuole membrane; endoplasmic reticulum; vacuolar transporter chaperone complex GO_0000329; GO_0005783; GO_0033254 6193 YPL020C ULP1 Protease that specifically cleaves Smt3p protein conjugates Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions C IDA; IDA; IDA nuclear envelope; nuclear pore; nucleolus GO_0005635; GO_0005643; GO_0005730 6194 YPL021W ECM23 Non-essential protein of unconfirmed function Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p 6195 YPL022W RAD1 Single-stranded DNA endonuclease (with Rad10p) Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein C IPI; IDA nucleotide-excision repair factor 1 complex; nucleus GO_0000110; GO_0005634 6196 YPL023C MET12 Protein with MTHFR activity in vitro Protein with MTHFR activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of methylenetetrahydrofolate reductase (MTHFR) 6197 YPL024W RMI1 Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase C HDA; IDA; HDA; IDA nucleus; RecQ helicase-Topo III complex; cytoplasm; nuclear replication fork GO_0005634; GO_0031422; GO_0005737; GO_0043596 6198 YPL025C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6199 YPL026C SKS1 Putative serine/threonine protein kinase Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication C IDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6200 YPL027W SMA1 Protein of unknown function involved in prospore membrane assembly Protein of unknown function involved in prospore membrane assembly; involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p C IDA prospore membrane GO_0005628 6201 YPL028W ERG10 Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase) Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis C HDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 6202 YPL029W SUV3 ATP-dependent RNA helicase ATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron C IDA; HDA; IDA mitochondrial chromosome; mitochondrion; mitochondrial degradosome GO_0000262; GO_0005739; GO_0045025 6203 YPL030W TRM44 tRNA(Ser) Um(44) 2'-O-methyltransferase tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene C HDA cytoplasm GO_0005737 6204 YPL031C PHO85 Cyclin-dependent kinase Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle C IPI; IDA; IDA cyclin-dependent protein kinase holoenzyme complex; Pho85-Pho80 CDK-cyclin complex; nucleus GO_0000307; GO_1990860; GO_0005634 6205 YPL032C SVL3 Protein of unknown function Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication C HDA; HDA; HDA; HDA; HDA cellular bud tip; cellular bud neck; cell periphery; cellular bud; cytoplasm GO_0005934; GO_0005935; GO_0071944; GO_0005933; GO_0005737 6206 YPL033C SRL4 Protein of unknown function Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p C HDA mitochondrion GO_0005739 6207 YPL034W YPL034W Putative protein of unknown function Putative protein of unknown function; YPL034W is not essential gene 6208 YPL035C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene 6209 YPL036W PMA2 Plasma membrane H+-ATPase Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential C HDA; IDA; HDA mitochondrion; plasma membrane; cell periphery GO_0005739; GO_0005886; GO_0071944 6210 YPL037C EGD1 Subunit beta1 of the nascent polypeptide-associated complex (NAC) Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication C IDA nascent polypeptide-associated complex GO_0005854 6211 YPL038W MET31 Zinc-finger DNA-binding transcription factor Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6212 YPL038W-A YPL038W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 6213 YPL039W YPL039W Putative protein of unknown function Putative protein of unknown function; YPL039W is not an essential gene 6214 YPL040C ISM1 Mitochondrial isoleucyl-tRNA synthetase Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth C HDA mitochondrion GO_0005739 6215 YPL041C MRX11 Protein that associates with mitochondrial ribosome Protein of unknown function involved in maintenance of telomere length C HDA mitochondrion GO_0005739 6216 YPL042C SSN3 Cyclin-dependent protein kinase Cyclin-dependent protein kinase; component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression C IDA mediator complex GO_0016592 6217 YPL043W NOP4 Nucleolar protein Nucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) C HDA; HDA; HDA nucleus; preribosome, large subunit precursor; nucleolus GO_0005634; GO_0030687; GO_0005730 6218 YPL044C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W 6219 YPL045W VPS16 Subunit of the HOPS and the CORVET complexes Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages C IDA; IDA; IPI CORVET complex; fungal-type vacuole membrane; HOPS complex GO_0033263; GO_0000329; GO_0030897 6220 YPL046C ELC1 Elongin C, conserved among eukaryotes Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair C IDA; IMP; IPI nucleotide-excision repair factor 4 complex; Cul3-RING ubiquitin ligase complex; elongin complex GO_0000113; GO_0031463; GO_0070449 6221 YPL047W SGF11 Integral subunit of SAGA histone acetyltransferase complex Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation C IDA; IDA; HDA SAGA complex; DUBm complex; nucleus GO_0000124; GO_0071819; GO_0005634 6222 YPL048W CAM1 One of two isoforms of the gamma subunit of eEF1B Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma C IDA; IDA cytoplasmic stress granule; nucleus GO_0010494; GO_0005634 6223 YPL049C DIG1 MAP kinase-responsive inhibitor of the Ste12p transcription factor MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication C IDA; IDA; IDA Tec1p-Ste12p-Dig1p complex; Ste12p-Dig1p-Dig2p complex; nucleus GO_1990527; GO_1990526; GO_0005634 6224 YPL050C MNN9 Subunit of Golgi mannosyltransferase complex Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p C IPI; IDA; HDA alpha-1,6-mannosyltransferase complex; cis-Golgi network; endoplasmic reticulum GO_0000136; GO_0005801; GO_0005783 6225 YPL051W ARL3 ARF-like small GTPase of the RAS superfamily ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1 C HDA; IDA; IDA cytosol; nuclear outer membrane-endoplasmic reticulum membrane network; Golgi apparatus GO_0005829; GO_0042175; GO_0005794 6226 YPL052W OAZ1 Regulator of ornithine decarboxylase Spe1p Regulator of ornithine decarboxylase Spe1p; antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p 6227 YPL053C KTR6 Probable mannosylphosphate transferase Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication C IDA; HDA membrane; fungal-type vacuole GO_0016020; GO_0000324 6228 YPL054W LEE1 Zinc-finger protein of unknown function Zinc-finger protein of unknown function 6229 YPL055C LGE1 Protein of unknown function Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division C HDA nucleus GO_0005634 6230 YPL056C LCL1 Putative protein of unknown function Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan 6231 YPL057C SUR1 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole; fungal-type vacuole membrane GO_0000324; GO_0000329 6232 YPL058C PDR12 Plasma membrane ATP-binding cassette (ABC) transporter Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity C IDA; HDA plasma membrane; cell periphery GO_0005886; GO_0071944 6233 YPL059W GRX5 Glutathione-dependent oxidoreductase Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p C IDA; HDA mitochondrial matrix; mitochondrion GO_0005759; GO_0005739 6234 YPL060W MFM1 Mitochondrial inner membrane magnesium transporter Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p C IDA mitochondrial inner membrane GO_0005743 6235 YPL061W ALD6 Cytosolic aldehyde dehydrogenase Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress C HDA; IMP mitochondrion; cytosol GO_0005739; GO_0005829 6236 YPL062W YPL062W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation 6237 YPL063W TIM50 Essential component of the TIM23 complex Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel C HDA; IDA mitochondrion; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0005744 6238 YPL064C CWC27 Component of a complex containing Cef1p Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress C IDA U2-type spliceosomal complex GO_0005684 6239 YPL065W VPS28 Component of the ESCRT-I complex Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p C IDA; HDA; IDA ESCRT I complex; cytosol; endosome GO_0000813; GO_0005829; GO_0005768 6240 YPL066W RGL1 Regulator of Rho1p signaling, cofactor of Tus1p Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source C HDA; HDA cellular bud neck; cytoplasm GO_0005935; GO_0005737 6241 YPL067C HTC1 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene C HDA cytoplasm GO_0005737 6242 YPL068C YPL068C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS C HDA nucleus GO_0005634 6243 YPL069C BTS1 Geranylgeranyl diphosphate synthase (GGPS) Geranylgeranyl diphosphate synthase; increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic C HDA mitochondrion GO_0005739 6244 YPL070W MUK1 Guanine nucleotide exchange factor (GEF) Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain C HDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 6245 YPL071C YPL071C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6246 YPL072W UBP16 Deubiquitinating enzyme anchored to the outer mitochondrial membrane Deubiquitinating enzyme anchored to the outer mitochondrial membrane; probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria C IDA; HDA mitochondrial outer membrane; mitochondrion GO_0005741; GO_0005739 6247 YPL073C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments 6248 YPL074W YTA6 Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress C IDA; HDA cell cortex; cytoplasm GO_0005938; GO_0005737 6249 YPL075W GCR1 Transcriptional activator of genes involved in glycolysis Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p C IDA nuclear envelope GO_0005635 6250 YPL076W GPI2 Protein involved in the synthesis of GlcNAc-PI Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol C HDA; IPI endoplasmic reticulum; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO_0005783; GO_0000506 6251 YPL077C YPL077C Putative protein of unknown function Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication 6252 YPL078C ATP4 Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated C IDA; HDA mitochondrial proton-transporting ATP synthase, stator stalk; mitochondrion GO_0000274; GO_0005739 6253 YPL079W RPL21B Ribosomal 60S subunit protein L21B Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6254 YPL080C YPL080C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6255 YPL081W RPS9A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication C HDA; HDA; IDA; IDA 90S preribosome; cytoplasm; cytosolic small ribosomal subunit; small-subunit processome GO_0030686; GO_0005737; GO_0022627; GO_0032040 6256 YPL082C MOT1 Essential protein involved in regulation of transcription Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA C IDA; HDA; HDA nuclear chromosome; nucleus; mitochondrion GO_0000228; GO_0005634; GO_0005739 6257 YPL083C SEN54 Subunit of the tRNA splicing endonuclease Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p C IMP; HDA; IDA mitochondrial outer membrane; mitochondrion; tRNA-intron endonuclease complex GO_0005741; GO_0005739; GO_0000214 6258 YPL084W BRO1 Cytoplasmic class E vacuolar protein sorting (VPS) factor Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes C HDA; IDA; IDA cytosol; endosome; cytoplasm GO_0005829; GO_0005768; GO_0005737 6259 YPL085W SEC16 COPII vesicle coat protein required for ER transport vesicle budding COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p C IDA; IDA endoplasmic reticulum exit site; ER to Golgi transport vesicle membrane GO_0070971; GO_0012507 6260 YPL086C ELP3 Subunit of Elongator complex Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin C IDA; IDA; IDA cytoplasm; Elongator holoenzyme complex; nucleus GO_0005737; GO_0033588; GO_0005634 6261 YPL087W YDC1 Alkaline dihydroceramidase, involved in sphingolipid metabolism Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication C IDA; HDA endoplasmic reticulum; fungal-type vacuole membrane GO_0005783; GO_0000329 6262 YPL088W YPL088W Putative aryl alcohol dehydrogenase Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance 6263 YPL089C RLM1 MADS-box transcription factor MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication C IDA nucleus GO_0005634 6264 YPL090C RPS6A Protein component of the small (40S) ribosomal subunit Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication C IDA; HDA; IDA cytosolic small ribosomal subunit; 90S preribosome; small-subunit processome GO_0022627; GO_0030686; GO_0032040 6265 YPL091W GLR1 Cytosolic and mitochondrial glutathione oxidoreductase Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress C IDA; IDA; HDA; HDA cytosol; nucleus; cytoplasm; mitochondrion GO_0005829; GO_0005634; GO_0005737; GO_0005739 6266 YPL092W SSU1 Plasma membrane sulfite pump involved in sulfite metabolism Plasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein C HDA; HDA; IDA endoplasmic reticulum; cell periphery; plasma membrane GO_0005783; GO_0071944; GO_0005886 6267 YPL093W NOG1 Putative GTPase Putative GTPase; associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins C IDA; IDA preribosome, large subunit precursor; nucleolus GO_0030687; GO_0005730 6268 YPL094C SEC62 Essential subunit of Sec63 complex Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec62p, Sec66p and Sec72p C HDA; IPI endoplasmic reticulum; Sec62/Sec63 complex GO_0005783; GO_0031207 6269 YPL095C EEB1 Acyl-coenzymeA:ethanol O-acyltransferase Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication 6270 YPL096C-A ERI1 Endoplasmic reticulum membrane protein that binds and inhibits Ras2p Endoplasmic reticulum membrane protein that binds and inhibits Ras2p; binds to and inhibits GTP-bound Ras2p at the endoplasmic reticulum (ER); component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein C HDA; IDA; IPI endoplasmic reticulum; endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO_0005783; GO_0005789; GO_0000506 6271 YPL096W PNG1 Conserved peptide N-glycanase Conserved peptide N-glycanase; required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p C IDA; HDA; IDA cytosol; mitochondrion; nucleus GO_0005829; GO_0005739; GO_0005634 6272 YPL097W MSY1 Mitochondrial tyrosyl-tRNA synthetase Mitochondrial tyrosyl-tRNA synthetase C HDA mitochondrion GO_0005739 6273 YPL098C MGR2 Subunit of the TIM23 translocase complex Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) C HDA; IPI mitochondrion; mitochondrial inner membrane presequence translocase complex GO_0005739; GO_0005744 6274 YPL099C INA17 F1F0 ATPase synthase peripheral stalk assembly factor F1F0 ATPase synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Ina22p and ATP synthase subunits; null mutant displays elevated frequency of mitochondrial genome loss and has a respiratory growth defect C HDA; IDA; IPI mitochondrion; intrinsic component of mitochondrial inner membrane; INA complex GO_0005739; GO_0031304; GO_1990524 6275 YPL100W ATG21 Phosphoinositide binding protein Phosphoinositide binding protein; required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein C IMP; HDA; IDA; IDA pre-autophagosomal structure; fungal-type vacuole membrane; cytosol; endosome GO_0000407; GO_0000329; GO_0005829; GO_0005768 6276 YPL101W ELP4 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity C IDA; HDA Elongator holoenzyme complex; cytoplasm GO_0033588; GO_0005737 6277 YPL102C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 6278 YPL103C FMP30 Protein with a role in maintaining mitochondrial morphology Protein with a role in maintaining mitochondrial morphology; also involved in maintaining normal cardiolipin levels; mitochondrial inner membrane protein; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D C HDA; IDA mitochondrion; integral component of mitochondrial inner membrane GO_0005739; GO_0031305 6279 YPL104W MSD1 Mitochondrial aspartyl-tRNA synthetase Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene C HDA mitochondrion GO_0005739 6280 YPL105C SYH1 Protein of unknown function that influences nuclear pore distribution Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication C IDA; IDA; HDA; HDA; IDA extrinsic component of membrane; P-body; ribosome; mitochondrion; cytoplasm GO_0019898; GO_0000932; GO_0005840; GO_0005739; GO_0005737 6281 YPL106C SSE1 ATPase component of heat shock protein Hsp90 chaperone complex ATPase component of heat shock protein Hsp90 chaperone complex; plays a role in determining prion variants; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication C HDA; IDA cytoplasm; polysome GO_0005737; GO_0005844 6282 YPL107W DPC25 Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene C HDA mitochondrion GO_0005739 6283 YPL108W YPL108W Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS C HDA cytoplasm GO_0005737 6284 YPL109C MCO76 UbiB family protein Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 6285 YPL110C GDE1 Glycerophosphocholine (GroPCho) phosphodiesterase Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes C HDA; HDA ribosome; cytoplasm GO_0005840; GO_0005737 6286 YPL111W CAR1 Arginase, catabolizes arginine to ornithine and urea Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance C HDA; IDA; HDA; HDA mating projection tip; cytosol; nucleus; cytoplasm GO_0043332; GO_0005829; GO_0005634; GO_0005737 6287 YPL112C PEX25 Peripheral peroxisomal membrane peroxin Peripheral peroxisomal membrane peroxin; required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with Pex27p; PEX25 has a paralog, PEX27, that arose from the whole genome duplication C IDA; IDA peroxisomal membrane; peroxisomal importomer complex GO_0005778; GO_1990429 6288 YPL113C YPL113C Glyoxylate reductase Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene 6289 YPL114W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index 6290 YPL115C BEM3 Rho GTPase activating protein (RhoGAP) Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly C IDA; IDA; IDA; IDA; IDA cytoplasm; cellular bud tip; mating projection tip; incipient cellular bud site; cell cortex GO_0005737; GO_0005934; GO_0043332; GO_0000131; GO_0005938 6291 YPL116W HOS3 Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats C HDA; HDA; HDA; HDA cellular bud neck; prospore membrane; nucleus; cytoplasm GO_0005935; GO_0005628; GO_0005634; GO_0005737 6292 YPL117C IDI1 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase Isopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; isopentenyl diphosphate:dimethylallyl diphosphate isomerase is also known as IPP isomerase C HDA; HDA; HDA cytosol; cytoplasm; nucleus GO_0005829; GO_0005737; GO_0005634 6293 YPL118W MRP51 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 6294 YPL119C DBP1 Putative ATP-dependent RNA helicase of the DEAD-box protein family Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6295 YPL119C-A YPL119C-A Putative protein of unknown function Putative protein of unknown function; identified by expression profiling and mass spectrometry 6296 YPL120W VPS30 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has a novel globular fold that is essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of the higher eukaryotic gene Beclin 1 C IDA; IDA; IDA; IDA; IDA; HDA; HDA phosphatidylinositol 3-kinase complex, class III, type II; extrinsic component of membrane; phosphatidylinositol 3-kinase complex, class III, type I; cytoplasm; NA; cytosol; fungal-type vacuole membrane GO_0034272; GO_0019898; GO_0034271; GO_0005737; GO_0120095; GO_0005829; GO_0000329 6297 YPL121C MEI5 Meiosis-specific protein involved in meiotic recombination Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p C IDA; HDA condensed nuclear chromosome; nucleus GO_0000794; GO_0005634 6298 YPL122C TFB2 Subunit of TFIIH and nucleotide excision repair factor 3 complexes Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH C IDA; IDA; IPI holo TFIIH complex; core TFIIH complex; nucleotide-excision repair factor 3 complex GO_0005675; GO_0000439; GO_0000112 6299 YPL123C RNY1 Vacuolar RNase of the T(2) family Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity C IDA; IDA; IDA; IDA fungal-type vacuole; cytosol; extracellular region; vacuole GO_0000324; GO_0005829; GO_0005576; GO_0005773 6300 YPL124W SPC29 Inner plaque spindle pole body (SPB) component Inner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication C HDA; IDA spindle pole body; central plaque of spindle pole body GO_0005816; GO_0005823 6301 YPL125W KAP120 Karyopherin responsible for the nuclear import of Rpf1p Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor C IDA; IDA; IDA cytoplasm; nuclear pore; nucleus GO_0005737; GO_0005643; GO_0005634 6302 YPL126W NAN1 U3 snoRNP protein U3 snoRNP protein; component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA C IDA; IDA; IDA; IDA; HDA rDNA heterochromatin; small-subunit processome; t-UTP complex; nucleolus; 90S preribosome GO_0033553; GO_0032040; GO_0034455; GO_0005730; GO_0030686 6303 YPL127C HHO1 Histone H1, linker histone with roles in meiosis and sporulation Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination C IDA; IDA; IPI nucleus; nuclear chromatin; nuclear nucleosome GO_0005634; GO_0000790; GO_0000788 6304 YPL128C TBF1 Telobox-containing general regulatory factor Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation C IDA; HDA nuclear chromosome, telomeric region; nucleus GO_0000784; GO_0005634 6305 YPL129W TAF14 Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain C IPI; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA mediator complex; Ino80 complex; SWI/SNF complex; NuA3a histone acetyltransferase complex; transcription factor TFIIF complex; transcription factor TFIID complex; NuA3b histone acetyltransferase complex; NuA3 histone acetyltransferase complex; nucleus GO_0016592; GO_0031011; GO_0016514; GO_1990467; GO_0005674; GO_0005669; GO_1990468; GO_0033100; GO_0005634 6306 YPL130W SPO19 Meiosis-specific prospore protein Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation; SPO19 has a paralog, YOR214C, that arose from the whole genome duplication C IDA; IDA prospore membrane; fungal-type cell wall GO_0005628; GO_0009277 6307 YPL131W RPL5 Ribosomal 60S subunit protein L5 Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly C IDA cytosolic large ribosomal subunit GO_0022625 6308 YPL132W COX11 Protein required for delivery of copper to Cox1p Protein required for delivery of copper to Cox1p; mitochondrial inner membrane protein; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p C HDA; IDA; IDA; IDA; IDA mitochondrion; mitochondrial envelope; mitochondrial intermembrane space; mitochondrial ribosome; mitochondrial inner membrane GO_0005739; GO_0005740; GO_0005758; GO_0005761; GO_0005743 6309 YPL133C RDS2 Transcription factor involved in regulating gluconeogenesis Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6310 YPL134C ODC1 Mitochondrial inner membrane transporter Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation; ODC1 has a paralog, ODC2, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial inner membrane GO_0005739; GO_0005743 6311 YPL135C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6312 YPL135W ISU1 Conserved protein of the mitochondrial matrix Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable; ISU1 has a paralog, ISU2, that arose from the whole genome duplication C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 6313 YPL136W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C 6314 YPL137C GIP3 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication C HDA; HDA; HDA; HDA cytoplasm; endoplasmic reticulum; ribosome; mitochondrion GO_0005737; GO_0005783; GO_0005840; GO_0005739 6315 YPL138C SPP1 Subunit of COMPASS (Set1C) Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia C IPI; IDA; IDA Set1C/COMPASS complex; nucleus; cytosol GO_0048188; GO_0005634; GO_0005829 6316 YPL139C UME1 Component of both the Rpd3S and Rpd3L histone deacetylase complexes Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; HDA Rpd3S complex; Rpd3L complex; cytoplasm; nucleus; Rpd3L-Expanded complex GO_0032221; GO_0033698; GO_0005737; GO_0005634; GO_0070210 6317 YPL140C MKK2 MAPKK involved in the protein kinase C signaling pathway MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication C IDA; IDA; HDA; IDA; IDA mating projection tip; cytoplasm; cytosol; cellular bud neck; cellular bud tip GO_0043332; GO_0005737; GO_0005829; GO_0005935; GO_0005934 6318 YPL141C FRK1 Protein kinase of unknown cellular role Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6319 YPL142C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit 6320 YPL143W RPL33A Ribosomal 60S subunit protein L33A Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6321 YPL144W POC4 Component of a heterodimeric Poc4p-Irc25p chaperone Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions C IDA; HDA intracellular; cytoplasm GO_0005622; GO_0005737 6322 YPL145C KES1 One of seven members of the yeast oxysterol binding protein family One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication C HDA; HDA; IDA; IDA cytosol; cytoplasm; Golgi membrane; extrinsic component of membrane GO_0005829; GO_0005737; GO_0000139; GO_0019898 6323 YPL146C NOP53 Nucleolar protein Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p and with the nucleolar proteins Nop17p and Nip7p; null mutant is viable but growth is severely impaired C IDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 6324 YPL147W PXA1 Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2p Subunit of a heterodimeric peroxisomal ABC transport complex; required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; complex also includes Pxa2p C IDA; IDA peroxisomal importomer complex; integral component of peroxisomal membrane GO_1990429; GO_0005779 6325 YPL148C PPT2 Phosphopantetheine:protein transferase (PPTase) Phosphopantetheine:protein transferase (PPTase); activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation C HDA mitochondrion GO_0005739 6326 YPL149W ATG5 Conserved protein involved in autophagy and the Cvt pathway Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; also involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner C IDA; IDA; IDA; IDA; HDA; IDA; IDA; IDA pre-autophagosomal structure; Atg12-Atg5-Atg16 complex; autophagosome; cytosol; nucleus; NA; membrane; NA GO_0000407; GO_0034274; GO_0005776; GO_0005829; GO_0005634; GO_0061908; GO_0016020; GO_0120095 6327 YPL150W YPL150W Protein kinase of unknown cellular role Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study 6328 YPL151C PRP46 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs C IDA; IPI spliceosomal complex; Prp19 complex GO_0005681; GO_0000974 6329 YPL152W RRD2 Peptidyl-prolyl cis/trans-isomerase Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress C IDA protein phosphatase type 2A complex GO_0000159 6330 YPL152W-A YPL152W-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6331 YPL153C RAD53 DNA damage response protein kinase DNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 6332 YPL154C PEP4 Vacuolar aspartyl protease (proteinase A) Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates C HDA; IDA; HDA mitochondrion; fungal-type vacuole; endoplasmic reticulum GO_0005739; GO_0000324; GO_0005783 6333 YPL155C KIP2 Kinesin-related motor protein involved in mitotic spindle positioning Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle C IDA; IDA; HDA cortical microtubule plus-end; cytoplasmic microtubule; mitochondrion GO_1903754; GO_0005881; GO_0005739 6334 YPL156C PRM4 Pheromone-regulated protein proposed to be involved in mating Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift C HDA fungal-type vacuole GO_0000324 6335 YPL157W TGS1 Trimethyl guanosine synthase, conserved nucleolar methyl transferase Trimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p C IDA nucleolus GO_0005730 6336 YPL158C AIM44 Protein that regulates Cdc42p and Rho1p Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress C IDA; HDA cellular bud neck; cellular bud tip GO_0005935; GO_0005934 6337 YPL159C PET20 Mitochondrial protein Mitochondrial protein; required for respiratory growth under some conditions and for stability of the mitochondrial genome C IDA mitochondrion GO_0005739 6338 YPL160W CDC60 Cytosolic leucyl tRNA synthetase Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA C IDA cytoplasm GO_0005737 6339 YPL161C BEM4 Protein involved in establishment of cell polarity and bud emergence Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6340 YPL162C YPL162C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology C HDA fungal-type vacuole membrane GO_0000329 6341 YPL163C SVS1 Cell wall and vacuolar protein Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication C IDA; HDA; HDA fungal-type cell wall; cell periphery; fungal-type vacuole GO_0009277; GO_0071944; GO_0000324 6342 YPL164C MLH3 Protein involved in DNA mismatch repair and meiotic recombination Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability C IPI; IPI MutLgamma complex; nucleus GO_0097587; GO_0005634 6343 YPL165C SET6 SET domain protein of unknown function SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability 6344 YPL166W ATG29 Autophagy-specific protein Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C HDA; HDA; IDA; HDA; IDA cytoplasm; cytosol; pre-autophagosomal structure; nucleus; ATG1/ULK1 kinase complex GO_0005737; GO_0005829; GO_0000407; GO_0005634; GO_1990316 6345 YPL167C REV3 Catalytic subunit of DNA polymerase zeta Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p C IDA; IPI; IDA zeta DNA polymerase complex; nuclear chromatin; mitochondrion GO_0016035; GO_0000790; GO_0005739 6346 YPL168W MRX4 Protein that associates with mitochondrial ribosome Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated C HDA mitochondrion GO_0005739 6347 YPL169C MEX67 Poly(A)RNA binding protein involved in nuclear mRNA export Poly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore; ortholog of human TAP C HDA; IPI; IDA; IDA preribosome, large subunit precursor; nuclear RNA export factor complex; cytoplasm; nuclear pore GO_0030687; GO_0042272; GO_0005737; GO_0005643 6348 YPL170W DAP1 Heme-binding protein Heme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis C IDA endosome GO_0005768 6349 YPL171C OYE3 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN) Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death 6350 YPL172C COX10 Heme A:farnesyltransferase Heme A:farnesyltransferase; catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders C HDA mitochondrion GO_0005739 6351 YPL173W MRPL40 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C IDA; HDA mitochondrial large ribosomal subunit; mitochondrion GO_0005762; GO_0005739 6352 YPL174C NIP100 Large subunit of the dynactin complex Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) C IDA; IDA; IDA; IDA dynactin complex; spindle pole body; astral microtubule; cell cortex GO_0005869; GO_0005816; GO_0000235; GO_0005938 6353 YPL175W SPT14 UDP-glycosyltransferase subunit of the GPI-GnT complex UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins C HDA; IMP cytosol; endoplasmic reticulum GO_0005829; GO_0005783 6354 YPL176C TRE1 Transferrin receptor-like protein Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication C HDA fungal-type vacuole GO_0000324 6355 YPL177C CUP9 Homeodomain-containing transcriptional repressor Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress 6356 YPL178W CBC2 Small subunit of the heterodimeric cap binding complex with Sto1p Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif C IDA; IDA nuclear cap binding complex; commitment complex GO_0005846; GO_0000243 6357 YPL179W PPQ1 Protein phosphatase that regulates the mating response Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family C HDA cytoplasm GO_0005737 6358 YPL180W TCO89 Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p) Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin C IDA; IPI; IDA; IDA fungal-type vacuole membrane; TORC1 complex; extrinsic component of cytoplasmic side of plasma membrane; extrinsic component of vacuolar membrane GO_0000329; GO_0031931; GO_0031234; GO_0000306 6359 YPL181W CTI6 Component of the Rpd3L histone deacetylase complex Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain C IDA; HDA Rpd3L complex; Rpd3L-Expanded complex GO_0033698; GO_0070210 6360 YPL182C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W 6361 YPL183C RTT10 WD40 domain-containing protein involved in endosomal recycling WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 C IDA; HDA endosome; cytoplasm GO_0005768; GO_0005737 6362 YPL183W-A RTC6 Protein involved in translation Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress 6363 YPL184C MRN1 RNA-binding protein that may be involved in translational regulation RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation C HDA; IDA; IDA; IDA cytoplasm; P-body; cytoplasmic stress granule; nucleus GO_0005737; GO_0000932; GO_0010494; GO_0005634 6364 YPL185W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C 6365 YPL186C UIP4 Protein that interacts with Ulp1p Protein that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope C IDA; HDA; IDA endoplasmic reticulum; mitochondrial outer membrane; nuclear envelope GO_0005783; GO_0005741; GO_0005635 6366 YPL187W MF(ALPHA)1 Mating pheromone alpha-factor, made by alpha cells Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor; MF(ALPHA)1 has a paralog, MF(ALPHA)2, that arose from the whole genome duplication C IDA extracellular region GO_0005576 6367 YPL188W POS5 Mitochondrial NADH kinase Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 6368 YPL189C-A COA2 Cytochrome oxidase assembly factor Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p C IDA mitochondrial matrix GO_0005759 6369 YPL189W GUP2 Probable membrane protein Probable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; GUP2 has a paralog, GUP1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 6370 YPL190C NAB3 RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p) RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability C IDA; IDA; IDA cytoplasm; nucleus; Nrd1 complex GO_0005737; GO_0005634; GO_0035649 6371 YPL191C YPL191C K48-specific deubiquitinating (DUB) enzyme Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication C HDA; HDA; HDA cytoplasm; endoplasmic reticulum; cell periphery GO_0005737; GO_0005783; GO_0071944 6372 YPL192C PRM3 Protein required for nuclear envelope fusion during karyogamy Protein required for nuclear envelope fusion during karyogamy; pheromone-regulated; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body C HDA; IDA; IDA; IDA nuclear periphery; extrinsic component of nuclear outer membrane; nuclear envelope; spindle pole body GO_0034399; GO_0031316; GO_0005635; GO_0005816 6373 YPL193W RSA1 Protein involved in the assembly of 60S ribosomal subunits Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly C IDA nucleoplasm GO_0005654 6374 YPL194W DDC1 DNA damage checkpoint protein DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress C IDA; IDA; HDA checkpoint clamp complex; condensed nuclear chromosome; nucleus GO_0030896; GO_0000794; GO_0005634 6375 YPL195W APL5 Delta adaptin-like subunit of the clathrin associated protein complex Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3 C IMP AP-3 adaptor complex GO_0030123 6376 YPL196W OXR1 Protein of unknown function required for oxidative damage resistance Protein of unknown function required for oxidative damage resistance; required for normal levels of resistance to oxidative damage; null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes C IDA mitochondrion GO_0005739 6377 YPL197C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B 6378 YPL198W RPL7B Ribosomal 60S subunit protein L7B Ribosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication C IDA; IDA; IDA cytoplasm; cytosolic large ribosomal subunit; nucleolus GO_0005737; GO_0022625; GO_0005730 6379 YPL199C YPL199C Putative protein of unknown function Putative protein of unknown function; predicted to be palmitoylated C HDA; HDA cell periphery; cytoplasm GO_0071944; GO_0005737 6380 YPL200W CSM4 Protein required for accurate chromosome segregation during meiosis Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements; functions with meiosis-specific telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2, that arose from the whole genome duplication C IDA; IDA nuclear chromosome, telomeric region; endoplasmic reticulum membrane GO_0000784; GO_0005789 6381 YPL201C YIG1 Protein that interacts with glycerol 3-phosphatase Protein that interacts with glycerol 3-phosphatase; plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol C IDA; IDA cytosol; nucleus GO_0005829; GO_0005634 6382 YPL202C AFT2 Iron-regulated transcriptional activator Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication C HDA mitochondrion GO_0005739 6383 YPL203W TPK2 cAMP-dependent protein kinase catalytic subunit cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA; IDA; IDA nuclear chromatin; P-body; nucleus; cAMP-dependent protein kinase complex; cytosol GO_0000790; GO_0000932; GO_0005634; GO_0005952; GO_0005829 6384 YPL204W HRR25 Conserved casein kinase Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta) C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; HDA; IDA; HDA peroxisomal importomer complex; plasma membrane; cellular bud neck; cellular bud tip; spindle pole body; pre-autophagosomal structure; nucleus; Golgi apparatus; chromosome, centromeric region; cytoplasm; monopolin complex; cytosol GO_1990429; GO_0005886; GO_0005935; GO_0005934; GO_0005816; GO_0000407; GO_0005634; GO_0005794; GO_0000775; GO_0005737; GO_0033551; GO_0005829 6385 YPL205C Hypothetical protein; deletion of locus affects telomere length 6386 YPL206C PGC1 Phosphatidylglycerol phospholipase C Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs C IDA; HDA lipid particle; mitochondrion GO_0005811; GO_0005739 6387 YPL207W TYW1 Iron-sulfer protein required for synthesis of Wybutosine modified tRNA Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron C HDA endoplasmic reticulum GO_0005783 6388 YPL208W RKM1 SET-domain lysine-N-methyltransferase SET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp) C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6389 YPL209C IPL1 Aurora kinase of chromosomal passenger complex Aurora kinase of conserved chromosomal passenger complex; mediates release on mono-oriented kinetochores from microtubules in meiosis I, also release of kinetochores from cluster at SPBs at meiosis exit; helps maintain condensed chromosomes during anaphase, early telophase; required for SPB cohesion and prevention of multipolar spindle formation; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest C IDA; HDA; IDA; IDA; HDA; IDA; HDA; IDA; IDA spindle microtubule; microtubule; condensed nuclear chromosome kinetochore; kinetochore microtubule; cytoplasm; spindle; nucleus; spindle midzone; chromosome passenger complex GO_0005876; GO_0005874; GO_0000778; GO_0005828; GO_0005737; GO_0005819; GO_0005634; GO_0051233; GO_0032133 6390 YPL210C SRP72 Core component of the signal recognition particle (SRP) Core component of the signal recognition particle (SRP); the SRP is a ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane C IDA signal recognition particle, endoplasmic reticulum targeting GO_0005786 6391 YPL211W NIP7 Nucleolar protein required for 60S ribosome subunit biogenesis Nucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p C HDA; HDA; IDA; IDA preribosome, large subunit precursor; nucleolus; cytoplasm; nucleus GO_0030687; GO_0005730; GO_0005737; GO_0005634 6392 YPL212C PUS1 tRNA:pseudouridine synthase tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication C IPI nucleus GO_0005634 6393 YPL213W LEA1 Component of U2 snRNP complex Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein C IDA; IDA; HDA U2 snRNP; U2-type prespliceosome; cytoplasm GO_0005686; GO_0071004; GO_0005737 6394 YPL214C THI6 Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase; required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern C IDA; HDA cytosol; cytoplasm GO_0005829; GO_0005737 6395 YPL215W CBP3 Mitochondrial protein required for assembly of cytochrome bc1 complex Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation C IDA; HDA; IDA; IPI; IDA fungal-type vacuole membrane; mitochondrion; mitochondrial ribosome; Cbp3p-Cbp6 complex; integral component of mitochondrial membrane GO_0000329; GO_0005739; GO_0005761; GO_0061671; GO_0032592 6396 YPL216W YPL216W Putative protein of unknown function Putative protein of unknown function; not an essential gene; YPL216W has a paralog, ITC1, that arose from the whole genome duplication 6397 YPL217C BMS1 GTPase required for ribosomal subunit synthesis and rRNA processing GTPase required for ribosomal subunit synthesis and rRNA processing; required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p C IDA; IDA; IDA; HDA; IDA nucleus; cytoplasm; 90S preribosome; mitochondrion; nucleolus GO_0005634; GO_0005737; GO_0030686; GO_0005739; GO_0005730 6398 YPL218W SAR1 ARF family GTPase GTPase, GTP-binding protein of the ARF family; component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity helping in vesicle formation C IDA; IDA; IDA COPII vesicle coat; endoplasmic reticulum exit site; ER-mitochondrion membrane contact site GO_0030127; GO_0070971; GO_0044233 6399 YPL219W PCL8 Cyclin Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication 6400 YPL220W RPL1A Ribosomal 60S subunit protein L1A Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal C IDA cytosolic large ribosomal subunit GO_0022625 6401 YPL221W FLC1 Flavin adenine dinucleotide transporter Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication C HDA; IDA; HDA fungal-type vacuole; endoplasmic reticulum; cellular bud neck GO_0000324; GO_0005783; GO_0005935 6402 YPL222C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6403 YPL222W FMP40 Putative protein of unknown function Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 6404 YPL223C GRE1 Hydrophilin essential in desiccation-rehydration process Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6405 YPL224C MMT2 Putative metal transporter involved in mitochondrial iron accumulation Putative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication C IDA mitochondrion GO_0005739 6406 YPL225W YPL225W Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; ribosome GO_0005737; GO_0005840 6407 YPL226W NEW1 ATP binding cassette protein ATP binding cassette protein; cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction C HDA; IDA; IDA; HDA mitochondrion; cytoplasm; polysome; preribosome, large subunit precursor GO_0005739; GO_0005737; GO_0005844; GO_0030687 6408 YPL227C ALG5 UDP-glucose:dolichyl-phosphate glucosyltransferase UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum C IDA endoplasmic reticulum membrane GO_0005789 6409 YPL228W CET1 RNA 5'-triphosphatase involved in mRNA 5' capping RNA 5'-triphosphatase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of a Cet1p homodimer and two molecules of the guanylyltransferase Ceg1p; Cet1p also has a role in regulation of RNA pol II pausing at promoter-proximal sites; interaction between Cet1p and Ceg1p is required for Ceg1p nuclear import; mammalian enzyme is a single bifunctional polypeptide; CET1 has a paralog, CTL1, that arose from the whole genome duplication C IPI mRNA cap methyltransferase complex GO_0031533 6410 YPL229W YPL229W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6411 YPL230W USV1 Putative transcription factor containing a C2H2 zinc finger Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication C IDA nucleus GO_0005634 6412 YPL231W FAS2 Alpha subunit of fatty acid synthetase Alpha subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities C HDA; HDA; HDA; IDA cytosol; mitochondrion; cytoplasm; fatty acid synthase complex GO_0005829; GO_0005739; GO_0005737; GO_0005835 6413 YPL232W SSO1 Plasma membrane t-SNARE Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; HDA prospore membrane; SNARE complex; plasma membrane; fungal-type vacuole membrane; cell periphery GO_0005628; GO_0031201; GO_0005886; GO_0000329; GO_0071944 6414 YPL233W NSL1 Essential component of the MIND kinetochore complex Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) C IDA; IDA; IDA kinetochore; spindle pole; nuclear MIS12/MIND complex GO_0000776; GO_0000922; GO_0000818 6415 YPL234C VMA11 Vacuolar ATPase V0 domain subunit c' Vacuolar ATPase V0 domain subunit c'; involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen C IDA; IDA; HDA; HDA fungal-type vacuole; integral component of membrane; cell periphery; fungal-type vacuole membrane GO_0000324; GO_0016021; GO_0071944; GO_0000329 6416 YPL235W RVB2 ATP-dependent DNA helicase, also known as reptin ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly C IPI; IDA; IDA; IDA; IPI; HDA R2TP complex; Swr1 complex; nuclear telomeric heterochromatin; nucleus; Ino80 complex; ASTRA complex GO_0097255; GO_0000812; GO_0005724; GO_0005634; GO_0031011; GO_0070209 6417 YPL236C ENV7 Vacuolar membrane protein kinase Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) C HDA fungal-type vacuole membrane GO_0000329 6418 YPL237W SUI3 Beta subunit of the translation initiation factor eIF2 Beta subunit of the translation initiation factor eIF2; involved in the identification of the start codon; proposed to be involved in mRNA binding C IDA; IDA; IDA multi-eIF complex; eukaryotic translation initiation factor 2 complex; eukaryotic 43S preinitiation complex GO_0043614; GO_0005850; GO_0016282 6419 YPL238C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W 6420 YPL239W YAR1 Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 6421 YPL240C HSP82 Hsp90 chaperone Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6422 YPL241C CIN2 GTPase-activating protein (GAP) for Cin4p GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 6423 YPL242C IQG1 Essential protein required for determination of budding pattern Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress C HDA; HDA cytoplasm; cellular bud neck GO_0005737; GO_0005935 6424 YPL243W SRP68 Core component of the signal recognition particle (SRP) complex Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress C HDA; HDA; IDA cytoplasm; nuclear periphery; signal recognition particle, endoplasmic reticulum targeting GO_0005737; GO_0034399; GO_0005786 6425 YPL244C HUT1 Protein with a role in UDP-galactose transport to the Golgi lumen Protein with a role in UDP-galactose transport to the Golgi lumen; has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 C HDA endoplasmic reticulum GO_0005783 6426 YPL245W YPL245W Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6427 YPL246C RBD2 Possible rhomboid protease Possible rhomboid protease; has similarity to eukaryotic rhomboid proteases including Pcp1p C IDA; HDA; HDA nuclear periphery; Golgi apparatus; COPI-coated vesicle GO_0034399; GO_0005794; GO_0030137 6428 YPL247C YPL247C Putative protein of unknown function Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6429 YPL248C GAL4 DNA-binding transcription factor required for activating GAL genes DNA-binding transcription factor required for activating GAL genes; responds to galactose; repressed by Gal80p and activated by Gal3p C IDA nucleus GO_0005634 6430 YPL249C GYP5 GTPase-activating protein (GAP) for yeast Rab family members GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication C IDA; HDA; IDA; IDA; IDA; IDA; HDA plasma membrane; cytosol; cellular bud tip; incipient cellular bud site; cellular bud neck; Golgi-associated vesicle; cytoplasm GO_0005886; GO_0005829; GO_0005934; GO_0000131; GO_0005935; GO_0005798; GO_0005737 6431 YPL249C-A RPL36B Ribosomal 60S subunit protein L36B Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6432 YPL250C ATG41 Protein of unknown function Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ICY2 has a paralog, ICY1, that arose from the whole genome duplication 6433 YPL250W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified ORF RPL36B/YPL249C-A; identified by fungal homology and RT-PCR 6434 YPL251W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C 6435 YPL252C YAH1 Ferredoxin of the mitochondrial matrix Ferredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin C IDA mitochondrial matrix GO_0005759 6436 YPL253C VIK1 Protein that forms a kinesin-14 heterodimeric motor with Kar3p Protein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication C IDA; IDA spindle pole body; kinesin complex GO_0005816; GO_0005871 6437 YPL254W HFI1 Adaptor protein required for structural integrity of the SAGA complex Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions C IPI; IDA; IDA Ada2/Gcn5/Ada3 transcription activator complex; SAGA complex; SLIK (SAGA-like) complex GO_0005671; GO_0000124; GO_0046695 6438 YPL255W BBP1 Protein required for the spindle pole body (SPB) duplication Protein required for the spindle pole body (SPB) duplication; localizes at the cytoplasmic side of the central plaque periphery of the SPB; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p C IDA central plaque of spindle pole body GO_0005823 6439 YPL256C CLN2 G1 cyclin involved in regulation of the cell cycle G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication C IDA; IDA; IDA cytoplasm; nucleus; cyclin-dependent protein kinase holoenzyme complex GO_0005737; GO_0005634; GO_0000307 6440 YPL257W YPL257W Putative protein of unknown function Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene 6441 YPL257W-A YPL257W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 6442 YPL257W-B YPL257W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 6443 YPL258C THI21 Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p 6444 YPL259C APM1 Mu1-like medium subunit of the AP-1 complex Mu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex C IPI AP-1 adaptor complex GO_0030121 6445 YPL260W CUB1 Conserved fungal gene linked to DNA repair and proteasome function Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6446 YPL261C YPL261C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YPL260W 6447 YPL262W FUM1 Fumarase Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria C IDA; IDA; IDA mitochondrion; cytosol; mitochondrial matrix GO_0005739; GO_0005829; GO_0005759 6448 YPL263C KEL3 Cytoplasmic protein of unknown function Cytoplasmic protein of unknown function C HDA cytoplasm GO_0005737 6449 YPL264C YPL264C Putative membrane protein of unknown function Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene 6450 YPL265W DIP5 Dicarboxylic amino acid permease Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress C HDA; HDA; HDA; HDA fungal-type vacuole; cell periphery; plasma membrane; fungal-type vacuole membrane GO_0000324; GO_0071944; GO_0005886; GO_0000329 6451 YPL266W DIM1 Essential 18S rRNA dimethylase (dimethyladenosine transferase) Essential 18S rRNA dimethylase (dimethyladenosine transferase); responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing C HDA 90S preribosome GO_0030686 6452 YPL267W ACM1 Pseudosubstrate inhibitor of the APC/C Pseudosubstrate inhibitor of the APC/C; suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein; the anaphase-promoting complex/cyclosome is also known as APC/C C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 6453 YPL268W PLC1 Phospholipase C Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation C IDA; IPI chromosome, centromeric region; condensed nuclear chromosome kinetochore GO_0000775; GO_0000778 6454 YPL269W KAR9 Spindle positioning factor Karyogamy protein; required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules; localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase C IDA; IDA; IDA; IDA; IDA; IDA; IDA condensed nuclear chromosome kinetochore; mating projection tip; nucleus; spindle pole body; cytoplasmic microtubule; cytoplasm; cell cortex GO_0000778; GO_0043332; GO_0005634; GO_0005816; GO_0005881; GO_0005737; GO_0005938 6455 YPL270W MDL2 Mitochondrial inner membrane half-type ABC transporter Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis C HDA; IDA; IDA mitochondrion; vacuolar membrane; mitochondrial inner membrane GO_0005739; GO_0005774; GO_0005743 6456 YPL271W ATP15 Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated C IDA; HDA mitochondrial proton-transporting ATP synthase, central stalk; mitochondrion GO_0005756; GO_0005739 6457 YPL272C PBI1 Putative protein of unknown function Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene 6458 YPL273W SAM4 S-adenosylmethionine-homocysteine methyltransferase S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6459 YPL274W SAM3 High-affinity S-adenosylmethionine permease High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 6460 YPL277C YPL277C Putative protein of unknown function Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels C IDA membrane GO_0016020 6461 YPL278C YPL278C Putative protein of unknown function Putative protein of unknown function; gene expression regulated by copper levels 6462 YPL279C FEX2 Protein involved in fluoride export Protein involved in fluoride export; nearly identical to FEX1, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins C HDA; IDA cell periphery; plasma membrane GO_0071944; GO_0005886 6463 YPL280W HSP32 Possible chaperone and cysteine protease Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer C IDA; IDA cytoplasmic stress granule; P-body GO_0010494; GO_0000932 6464 YPL281C ERR2 Enolase, a phosphopyruvate hydratase Enolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant 6465 YPL282C PAU22 Protein of unknown function Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau21p; encodes 2 proteins that are translated from 2 different start codons C HDA fungal-type vacuole GO_0000324 6466 YPL283C YRF1-7 Helicase encoded by the Y' element of subtelomeric regions Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p C HDA mitochondrion GO_0005739 6467 YPL283W-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6468 YPL283W-B Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6469 YPR001W CIT3 Dual specificity mitochondrial citrate and methylcitrate synthase Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate C IDA mitochondrion GO_0005739 6470 YPR002C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the Ty1 long terminal repeat, YPRWdelta12 6471 YPR002W PDH1 Putative 2-methylcitrate dehydratase Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate C HDA; HDA; HDA mitochondrial outer membrane; cytoplasm; mitochondrion GO_0005741; GO_0005737; GO_0005739 6472 YPR003C YPR003C Putative sulfate permease Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene C HDA endoplasmic reticulum GO_0005783 6473 YPR004C AIM45 Putative ortholog of mammalian ETF-alpha Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit C HDA mitochondrion GO_0005739 6474 YPR005C HAL1 Cytoplasmic protein involved in halotolerance Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p C IDA cytoplasm GO_0005737 6475 YPR006C ICL2 2-methylisocitrate lyase of the mitochondrial matrix 2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol C HDA; IDA mitochondrion; mitochondrial matrix GO_0005739; GO_0005759 6476 YPR007C REC8 Meiosis-specific component of the sister chromatid cohesion complex Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; independent of its role in sister chromatid cohesion, Rec8p promotes allelic collisions and prevents nonspecific chromosome interactions; homolog of S. pombe Rec8p C IDA; IPI condensed nuclear chromosome, centromeric region; condensed nuclear chromosome GO_0000780; GO_0000794 6477 YPR008W HAA1 Transcriptional activator involved in adaptation to weak acid stress Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress C HDA; IDA cytoplasm; nucleus GO_0005737; GO_0005634 6478 YPR009W SUT2 Zn2Cys6 family transcription factor Putative transcription factor of the Zn2Cys6 family; regulates sterol uptake under anaerobic conditions along with SUT1; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; positively regulates mating along with SUT1 by repressing the expression of genes (PRR2, NCE102 and RHO5) which function as mating inhibitors; SUT2 has a paralog, SUT1, that arose from the whole genome duplication C IDA nucleus GO_0005634 6479 YPR010C RPA135 RNA polymerase I second largest subunit A135 RNA polymerase I second largest subunit A135 C IDA DNA-directed RNA polymerase I complex GO_0005736 6480 YPR010C-A MIN8 Putative protein of unknown function Putative protein of unknown function; conserved among Saccharomyces sensu stricto species C HDA cytosol GO_0005829 6481 YPR011C YPR011C Mitochondrial transporter Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies C HDA mitochondrion GO_0005739 6482 YPR012W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene 6483 YPR013C CMR3 Putative zinc finger protein Putative zinc finger protein; YPR013C is not an essential gene 6484 YPR014C YPR014C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene 6485 YPR015C YPR015C Putative zinc finger transcription factor Putative protein of unknown function; overexpression causes a cell cycle delay or arrest 6486 YPR016C TIF6 Constituent of 66S pre-ribosomal particles Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits C IDA; IDA; IDA; IDA preribosome, large subunit precursor; nucleus; cytoplasm; nucleolus GO_0030687; GO_0005634; GO_0005737; GO_0005730 6487 YPR016W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6488 YPR017C DSS4 Guanine nucleotide dissociation stimulator for Sec4p Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol C HDA; IDA cytosol; membrane GO_0005829; GO_0016020 6489 YPR018W RLF2 Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription C IDA; IDA; IDA; IDA CAF-1 complex; chromosome, centromeric region; chromatin; nucleus GO_0033186; GO_0000775; GO_0000785; GO_0005634 6490 YPR019W MCM4 Essential helicase component of heterohexameric MCM2-7 complexes Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p C IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA; IDA DNA replication preinitiation complex; replication fork protection complex; nuclear pre-replicative complex; cytoplasm; MCM core complex; CMG complex; MCM complex; nuclear replication fork; nucleus GO_0031261; GO_0031298; GO_0005656; GO_0005737; GO_0097373; GO_0071162; GO_0042555; GO_0043596; GO_0005634 6491 YPR020W ATP20 Subunit g of the mitochondrial F1F0 ATP synthase Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae C IPI; HDA mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); mitochondrion GO_0000276; GO_0005739 6492 YPR021C AGC1 Mitochondrial amino acid transporter Mitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis C HDA; IDA mitochondrion; vacuole-mitochondrion membrane contact site GO_0005739; GO_1990816 6493 YPR022C SDD4 Putative transcription factor, as suggested by computational analysis Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6494 YPR023C EAF3 Component of the Rpd3S histone deacetylase complex Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition C IDA; IDA; IDA Rpd3S complex; NuA4 histone acetyltransferase complex; histone acetyltransferase complex GO_0032221; GO_0035267; GO_0000123 6495 YPR024W YME1 Catalytic subunit of i-AAA protease complex Catalytic subunit of the i-AAA protease complex; complex is located in the mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as a nonconventional translocation motor to pull PNPase into the intermembrane space; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover C HDA; IDA; IDA mitochondrion; i-AAA complex; mitochondrial inner membrane GO_0005739; GO_0031942; GO_0005743 6496 YPR025C CCL1 Cyclin associated with protein kinase Kin28p Cyclin associated with protein kinase Kin28p; Kin28p is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters C IDA; IDA; HDA holo TFIIH complex; TFIIK complex; mitochondrion GO_0005675; GO_0070985; GO_0005739 6497 YPR026W ATH1 Acid trehalase required for utilization of extracellular trehalose Acid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress C IDA; IMP; IDA fungal-type cell wall; fungal-type vacuole; cell wall-bounded periplasmic space GO_0009277; GO_0000324; GO_0030287 6498 YPR027C YPR027C Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 6499 YPR028W YOP1 Reticulon-interacting protein Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress C HDA; HDA; HDA; HDA; IDA; IDA endoplasmic reticulum; cell periphery; cellular bud tip; cellular bud; endoplasmic reticulum tubular network; integral component of membrane GO_0005783; GO_0071944; GO_0005934; GO_0005933; GO_0071782; GO_0016021 6500 YPR029C APL4 Gamma-adaptin Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport C IPI AP-1 adaptor complex GO_0030121 6501 YPR030W CSR2 Nuclear ubiquitin protein ligase binding protein Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication C IDA; HDA nucleus; cytosol GO_0005634; GO_0005829 6502 YPR031W NTO1 Subunit of the NuA3 histone acetyltransferase complex Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 C HDA; IDA; IDA; IDA; HDA cytoplasm; NuA3b histone acetyltransferase complex; NuA3 histone acetyltransferase complex; NuA3a histone acetyltransferase complex; nucleus GO_0005737; GO_1990468; GO_0033100; GO_1990467; GO_0005634 6503 YPR032W SRO7 Effector of Rab GTPase Sec4p Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication C IDA; IDA; IDA cytosol; mating projection tip; plasma membrane GO_0005829; GO_0043332; GO_0005886 6504 YPR033C HTS1 Cytoplasmic and mitochondrial histidine tRNA synthetase Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome C IMP; IMP mitochondrion; cytoplasm GO_0005739; GO_0005737 6505 YPR034W ARP7 Component of both the SWI/SNF and RSC chromatin remodeling complexes Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation C IDA; IDA; IDA SWI/SNF complex; RSC complex; nucleus GO_0016514; GO_0016586; GO_0005634 6506 YPR035W GLN1 Glutamine synthetase (GS) Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; forms filaments of back-to-back stacks of cylindrical homo-decamers at low pH, leading to enzymatic inactivation and storage during states of advanced cellular starvation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C IDA; HDA; HDA nuclear periphery; nucleus; cytoplasm GO_0034399; GO_0005634; GO_0005737 6507 YPR036W VMA13 Subunit H of the V1 peripheral membrane domain of V-ATPase Subunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits C HDA fungal-type vacuole membrane GO_0000329 6508 YPR036W-A SPO24 Small (67 amino acids) protein involved in sporulation Small (67 amino acids) protein involved in sporulation; localizes to the prospore membrane; phosphorylated during meiosis; a longer, 5'-extended mRNA is also transcribed beginning in mid-meiosis, regulated by two MSEs (middle sporulation elements), and includes an uORF of 15 codons in its 5'-UTR; evidence transcription is regulated by Pdr1p C IDA prospore membrane GO_0005628 6509 YPR037C ERV2 Flavin-linked sulfhydryl oxidase localized to the ER lumen Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER) C HDA; IDA; HDA fungal-type vacuole membrane; integral component of endoplasmic reticulum membrane; fungal-type vacuole GO_0000329; GO_0030176; GO_0000324 6510 YPR038W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci 6511 YPR039W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W 6512 YPR040W TIP41 Protein that interacts with Tap42p, which regulates PP2A Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6513 YPR041W TIF5 Translation initiation factor eIF5 Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 C IPI; IDA; IDA cytosolic small ribosomal subunit; multi-eIF complex; eukaryotic 48S preinitiation complex GO_0022627; GO_0043614; GO_0033290 6514 YPR042C PUF2 PUF family mRNA-binding protein PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6515 YPR043W RPL43A Ribosomal 60S subunit protein L43A Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6516 YPR044C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO 6517 YPR045C THP3 Protein that may have a role in transcription elongation Protein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes C IDA; HDA transcriptionally active chromatin; nucleus GO_0035327; GO_0005634 6518 YPR046W MCM16 Component of the Ctf19 complex and the COMA subcomplex Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 C IDA condensed nuclear chromosome kinetochore GO_0000778 6519 YPR047W MSF1 Mitochondrial phenylalanyl-tRNA synthetase Mitochondrial phenylalanyl-tRNA synthetase; active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase C HDA mitochondrion GO_0005739 6520 YPR048W TAH18 Conserved NAPDH-dependent diflavin reductase Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance; possible target for development of antifungal drugs C IDA mitochondrion GO_0005739 6521 YPR049C ATG11 Adapter protein for pexophagy and the Cvt targeting pathway Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy C IDA; IDA pre-autophagosomal structure; extrinsic component of membrane GO_0000407; GO_0019898 6522 YPR050C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF MAK3/YPR051W 6523 YPR051W MAK3 Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT) Catalytic subunit of the NatC type N-terminal acetyltransferase; involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus C IDA NatC complex GO_0031417 6524 YPR052C NHP6A High-mobility group (HMG) protein High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress C IDA; IDA nucleus; MutSalpha complex GO_0005634; GO_0032301 6525 YPR053C YPR053C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C 6526 YPR054W SMK1 Middle sporulation-specific mitogen-activated protein kinase (MAPK) Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p C HDA mitochondrion GO_0005739 6527 YPR055W SEC8 Essential 121 kDa subunit of the exocyst complex Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress C IDA; IDA; IDA; HDA; HDA; HDA; IDA cellular bud tip; exocyst; cellular bud neck; mating projection tip; prospore membrane; cytoplasm; incipient cellular bud site GO_0005934; GO_0000145; GO_0005935; GO_0043332; GO_0005628; GO_0005737; GO_0000131 6528 YPR056W TFB4 Subunit of TFIIH complex Subunit of TFIIH complex; involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p C IPI; IDA; IDA nucleotide-excision repair factor 3 complex; core TFIIH complex; holo TFIIH complex GO_0000112; GO_0000439; GO_0005675 6529 YPR057W BRR1 snRNP protein component of spliceosomal snRNPs snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed C IDA small nuclear ribonucleoprotein complex GO_0030532 6530 YPR058W YMC1 Secondary mitochondrial inner membrane glycine transporter Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; localizes to vacuole in response to H2O2; YMC1 has a paralog, YMC2, that arose from the whole genome duplication C HDA; IDA mitochondrion; fungal-type vacuole GO_0005739; GO_0000324 6531 YPR059C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W 6532 YPR060C ARO7 Chorismate mutase Chorismate mutase; catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6533 YPR061C JID1 Probable Hsp40p co-chaperone Probable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae C HDA mitochondrion GO_0005739 6534 YPR062W FCY1 Cytosine deaminase Cytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6535 YPR063C YPR063C ER-localized protein of unknown function ER-localized protein of unknown function C HDA endoplasmic reticulum GO_0005783 6536 YPR064W YPR064W Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6537 YPR065W ROX1 Heme-dependent repressor of hypoxic genes Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance 6538 YPR066W UBA3 Protein that activates Rub1p (NEDD8) before neddylation Protein that activates Rub1p (NEDD8) before neddylation; acts together with Ula1p; may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern C HDA cytoplasm GO_0005737 6539 YPR067W ISA2 Protein required for maturation of mitochondrial [4Fe-4S] proteins Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations C IDA; IDA; HDA mitochondrial matrix; mitochondrial intermembrane space; mitochondrion GO_0005759; GO_0005758; GO_0005739 6540 YPR068C HOS1 Class I histone deacetylase (HDAC) family member Class I histone deacetylase (HDAC) family member; deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex C IPI histone deacetylase complex GO_0000118 6541 YPR069C SPE3 Spermidine synthase Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6542 YPR070W MED1 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation C IDA core mediator complex GO_0070847 6543 YPR071W YPR071W Putative membrane protein Putative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication C HDA; HDA endoplasmic reticulum; cell periphery GO_0005783; GO_0071944 6544 YPR072W NOT5 Component of the CCR4-NOT core complex, involved in mRNA decapping Subunit of CCR4-NOT global transcriptional regulator; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers C IDA; IPI cytoplasm; CCR4-NOT core complex GO_0005737; GO_0030015 6545 YPR073C LTP1 Protein phosphotyrosine phosphatase of unknown cellular role Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6546 YPR074C TKL1 Transketolase Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication 6547 YPR074W-A Hypothetical protein identified by homology 6548 YPR075C OPY2 Integral membrane protein that acts as a membrane anchor for Ste50p Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress C HDA; HDA; HDA; HDA; IDA; HDA fungal-type vacuole; plasma membrane; fungal-type vacuole membrane; cytoplasm; integral component of plasma membrane; endoplasmic reticulum GO_0000324; GO_0005886; GO_0000329; GO_0005737; GO_0005887; GO_0005783 6549 YPR076W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6550 YPR077C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 6551 YPR078C YPR078C Putative protein of unknown function Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible 6552 YPR079W MRL1 Membrane protein Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress C IDA; HDA late endosome; cytoplasm GO_0005770; GO_0005737 6553 YPR080W TEF1 Translational elongation factor EF-1 alpha Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF1 has a paralog, TEF2, that arose from the whole genome duplication C IDA; IPI; HDA; IDA fungal-type vacuole membrane; mitochondrion; cytosol; cytoplasm GO_0000329; GO_0005739; GO_0005829; GO_0005737 6554 YPR081C GRS2 Glycine-tRNA synthetase, not expressed under normal growth conditions Glycine-tRNA synthetase, not expressed under normal growth conditions; expression is induced under heat, oxidative, pH, or ethanol stress conditions; more stable than the major glycine-tRNA synthetase Grs1p at 37 deg C; GRS2 has a paralog, GRS1, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6555 YPR082C DIB1 17-kDa component of the U4/U6aU5 tri-snRNP 17-kDa component of the U4/U6aU5 tri-snRNP; plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein C IDA; IDA U4/U6 x U5 tri-snRNP complex; U5 snRNP GO_0046540; GO_0005682 6556 YPR083W MDM36 Mitochondrial protein Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission C IDA; IDA mitochondrion; cytoplasm GO_0005739; GO_0005737 6557 YPR084W YPR084W Putative protein of unknown function Putative protein of unknown function 6558 YPR085C ASA1 Subunit of the ASTRA complex, involved in chromatin remodeling Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 C HDA ASTRA complex GO_0070209 6559 YPR086W SUA7 Transcription factor TFIIB Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II C IDA; IDA nucleus; transcriptional preinitiation complex GO_0005634; GO_0097550 6560 YPR087W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect 6561 YPR088C SRP54 Signal recognition particle (SRP) subunit (homolog of mammalian SRP54) Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain C HDA; IDA endoplasmic reticulum; signal recognition particle, endoplasmic reticulum targeting GO_0005783; GO_0005786 6562 YPR089W YPR089W Protein of unknown function Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p 6563 YPR091C NVJ2 Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ) Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments C HDA; IDA; HDA; HDA endoplasmic reticulum; nucleus-vacuole junction; cell periphery; ribosome GO_0005783; GO_0071561; GO_0071944; GO_0005840 6564 YPR092W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6565 YPR093C ASR1 Ubiquitin ligase that modifies and regulates RNA Pol II Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 6566 YPR094W RDS3 Component of the SF3b subcomplex of the U2 snRNP Component of the SF3b subcomplex of the U2 snRNP; zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance C IDA U2 snRNP GO_0005686 6567 YPR095C SYT1 Guanine nucleotide exchange factor (GEF) for Arf proteins Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain C HDA; IDA mitochondrion; Golgi membrane GO_0005739; GO_0000139 6568 YPR096C YPR096C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments C HDA ribosome GO_0005840 6569 YPR097W YPR097W Protein that contains a PX domain and binds phosphoinositides Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology C HDA mitochondrion GO_0005739 6570 YPR098C TMH18 Protein of unknown function Protein of unknown function; localized to the mitochondrial outer membrane C HDA; HDA mitochondrion; mitochondrial outer membrane GO_0005739; GO_0005741 6571 YPR099C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W 6572 YPR100W MRPL51 Mitochondrial ribosomal protein of the large subunit Mitochondrial ribosomal protein of the large subunit C HDA; IDA mitochondrion; mitochondrial large ribosomal subunit GO_0005739; GO_0005762 6573 YPR101W SNT309 Member of the NineTeen Complex (NTC) Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p C IDA; IDA U2-type catalytic step 1 spliceosome; Prp19 complex GO_0071006; GO_0000974 6574 YPR102C RPL11A Ribosomal 60S subunit protein L11A Ribosomal 60S subunit protein L11A; expressed at twice the level of Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11A has a paralog, RPL11B, that arose from the whole genome duplication C IDA cytosolic large ribosomal subunit GO_0022625 6575 YPR103W PRE2 Beta 5 subunit of the 20S proteasome Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome C HDA; HDA; IDA nucleus; cytosol; proteasome core complex, beta-subunit complex GO_0005634; GO_0005829; GO_0019774 6576 YPR104C FHL1 Regulator of ribosomal protein (RP) transcription Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants C IDA; IDA; IDA; IDA nucleolus; CURI complex; nuclear chromatin; nucleus GO_0005730; GO_0032545; GO_0000790; GO_0005634 6577 YPR105C COG4 Essential component of the conserved oligomeric Golgi complex Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments C IMP; HDA Golgi transport complex; cytosol GO_0017119; GO_0005829 6578 YPR106W ISR1 Predicted protein kinase Predicted protein kinase; overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C 6579 YPR107C YTH1 Essential RNA-binding component of cleavage and polyadenylation factor Essential RNA-binding component of cleavage and polyadenylation factor; contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation; relocalizes to the cytosol in response to hypoxia C IDA; IDA; IDA cytosol; nucleus; mRNA cleavage and polyadenylation specificity factor complex GO_0005829; GO_0005634; GO_0005847 6580 YPR108W RPN7 Essential non-ATPase regulatory subunit of the 26S proteasome Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits C IDA; IDA proteasome regulatory particle, lid subcomplex; nucleus GO_0008541; GO_0005634 6581 YPR108W-A YPR108W-A Putative protein of unknown function Putative protein of unknown function; identified by fungal homology and RT-PCR 6582 YPR109W GLD1 Predicted membrane protein Predicted membrane protein; diploid deletion strain has high budding index C HDA endoplasmic reticulum GO_0005783 6583 YPR110C RPC40 RNA polymerase subunit AC40 RNA polymerase subunit AC40; common to RNA polymerase I and III C IDA; IDA DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase I complex GO_0005666; GO_0005736 6584 YPR111W DBF20 Ser/Thr kinase involved in late nuclear division Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication C IDA; HDA; IDA cellular bud neck; cytoplasm; spindle pole body GO_0005935; GO_0005737; GO_0005816 6585 YPR112C MRD1 Essential conserved small ribosomal subunit (40s) synthesis factor Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region C IDA; IDA 90S preribosome; nucleolus GO_0030686; GO_0005730 6586 YPR113W PIS1 Phosphatidylinositol synthase Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins C HDA; IDA; IDA; IDA; HDA mitochondrion; Golgi apparatus; integral component of membrane; mitochondrial outer membrane; endoplasmic reticulum GO_0005739; GO_0005794; GO_0016021; GO_0005741; GO_0005783 6587 YPR114W YPR114W Putative protein of unknown function Putative protein of unknown function C HDA endoplasmic reticulum GO_0005783 6588 YPR115W RGC1 Putative regulator of the Fps1p glycerol channel Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6589 YPR116W RRG8 Protein of unknown function Putative protein of unknown function; required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport C HDA mitochondrion GO_0005739 6590 YPR117W YPR117W Putative protein of unknown function Putative protein of unknown function 6591 YPR118W MRI1 5'-methylthioribose-1-phosphate isomerase 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6592 YPR119W CLB2 B-type cyclin involved in cell cycle progression B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication C IDA; IMP; IDA; IDA; IDA spindle; cytoplasm; spindle pole body; nucleus; cellular bud neck GO_0005819; GO_0005737; GO_0005816; GO_0005634; GO_0005935 6593 YPR120C CLB5 B-type cyclin involved in DNA replication during S phase B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication C IDA nucleus GO_0005634 6594 YPR121W THI22 Protein with similarity to hydroxymethylpyrimidine phosphate kinases Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis 6595 YPR122W AXL1 Haploid specific endoprotease of a-factor mating pheromone Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells C IDA; IDA mating projection tip; cellular bud neck GO_0043332; GO_0005935 6596 YPR123C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR 6597 YPR124W CTR1 High-affinity copper transporter of plasma membrane High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress C HDA; HDA; IDA cytoplasm; nucleus; plasma membrane GO_0005737; GO_0005634; GO_0005886 6598 YPR125W YLH47 Mitochondrial inner membrane protein Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome C IDA; HDA mitochondrial inner membrane; mitochondrion GO_0005743; GO_0005739 6599 YPR126C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6600 YPR127W YPR127W Putative pyridoxine 4-dehydrogenase Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus C HDA; HDA cytoplasm; nucleus GO_0005737; GO_0005634 6601 YPR128C ANT1 Peroxisomal adenine nucleotide transporter Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation C HDA; IDA cytoplasm; integral component of peroxisomal membrane GO_0005737; GO_0005779 6602 YPR129W SCD6 Repressor of translation initiation Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress C HDA; IDA; IDA cytoplasm; cytoplasmic stress granule; P-body GO_0005737; GO_0010494; GO_0000932 6603 YPR130C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6604 YPR131C NAT3 Catalytic subunit of the NatB N-terminal acetyltransferase Catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met C HDA; IDA cytoplasm; NatB complex GO_0005737; GO_0031416 6605 YPR132W RPS23B Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal C IDA cytosolic small ribosomal subunit GO_0022627 6606 YPR133C SPN1 Protein involved in RNA polymerase II transcription Protein involved in RNA polymerase II transcription; is constitutively recruited to the CYC1 promoter and is required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype 6607 YPR133W-A TOM5 Component of the TOM (translocase of outer membrane) complex Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore C HDA; IDA; HDA; IDA mitochondrial outer membrane; integral component of mitochondrial outer membrane; mitochondrion; mitochondrial outer membrane translocase complex GO_0005741; GO_0031307; GO_0005739; GO_0005742 6608 YPR134W MSS18 Nuclear encoded protein needed for splicing of mitochondrial intron Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions C HDA mitochondrion GO_0005739 6609 YPR135W CTF4 Chromatin-associated protein Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion C IDA; IPI; IDA; IDA nuclear replication fork; nucleus; nuclear chromosome; replication fork protection complex GO_0043596; GO_0005634; GO_0000228; GO_0031298 6610 YPR136C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 6611 YPR137C-A YPR137C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 6612 YPR137C-B YPR137C-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 6613 YPR137W RRP9 Protein involved in pre-rRNA processing Protein involved in pre-rRNA processing; associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein C HDA; IDA; IDA; IDA 90S preribosome; small-subunit processome; box C/D snoRNP complex; nucleolus GO_0030686; GO_0032040; GO_0031428; GO_0005730 6614 YPR138C MEP3 Ammonium permease of high capacity and low affinity Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease; MEP3 has a paralog, MEP1, that arose from the whole genome duplication C HDA endoplasmic reticulum GO_0005783 6615 YPR139C LOA1 Lysophosphatidic acid acyltransferase Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA C HDA; HDA; IDA endoplasmic reticulum; cytoplasm; lipid particle GO_0005783; GO_0005737; GO_0005811 6616 YPR140W TAZ1 Lyso-phosphatidylcholine acyltransferase Lyso-phosphatidylcholine acyltransferase; required for normal phospholipid content of mitochondrial membranes; major determinant of the final acyl chain composition of the mitochondrial-specific phospholipid cardiolipin; mutations in human ortholog tafazzin cause Barth syndrome, a rare X-linked disease characterized by skeletal and cardiomyopathy and bouts of cyclic neutropenia C HDA; IDA; IDA; IDA mitochondrion; integral component of mitochondrial outer membrane; mitochondrial inner membrane; mitochondrial outer membrane GO_0005739; GO_0031307; GO_0005743; GO_0005741 6617 YPR141C KAR3 Minus-end-directed microtubule motor Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate C IDA; HDA; HDA; IDA; HDA spindle pole body; nucleus; spindle pole; cytoplasmic microtubule; microtubule GO_0005816; GO_0005634; GO_0000922; GO_0005881; GO_0005874 6618 YPR142C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing 6619 YPR143W RRP15 Nucleolar protein Nucleolar protein; constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs C HDA; IDA; HDA nucleus; preribosome, large subunit precursor; nucleolus GO_0005634; GO_0030687; GO_0005730 6620 YPR144C NOC4 Nucleolar protein Nucleolar protein; forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits; relocalizes to the cytosol in response to hypoxia C IDA; IDA; HDA; IDA; IPI; IDA nucleus; cytosol; 90S preribosome; nucleolus; Noc4p-Nop14p complex; small-subunit processome GO_0005634; GO_0005829; GO_0030686; GO_0005730; GO_0030692; GO_0032040 6621 YPR145C-A YPR145C-A Putative protein of unknown function Putative protein of unknown function 6622 YPR145W ASN1 Asparagine synthetase Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication C HDA cytoplasm GO_0005737 6623 YPR146C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6624 YPR147C YPR147C Bifunctional triacylglycerol lipase and short chain ester hydrolase Putative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS C IDA; HDA lipid particle; cytoplasm GO_0005811; GO_0005737 6625 YPR148C YPR148C Protein of unknown function Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern C HDA; HDA ribosome; cytoplasm GO_0005840; GO_0005737 6626 YPR149W NCE102 Protein of unknown function Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication C IDA; HDA; IDA; HDA; IDA; HDA; HDA; IDA cellular bud membrane; cell periphery; plasma membrane; cytoplasm; membrane raft; mitochondrion; endoplasmic reticulum; mating projection membrane GO_0033101; GO_0071944; GO_0005886; GO_0005737; GO_0045121; GO_0005739; GO_0005783; GO_0070250 6627 YPR150W Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C 6628 YPR151C SUE1 Protein required for degradation of unstable forms of cytochrome c Protein required for degradation of unstable forms of cytochrome c; located in the mitochondria C IDA mitochondrial envelope GO_0005740 6629 YPR152C URN1 Protein of unknown function containing WW and FF domains Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase C HDA nucleus GO_0005634 6630 YPR153W MAY24 Protein of unknown function Putative protein of unknown function 6631 YPR154W PIN3 Negative regulator of actin nucleation-promoting factor activity Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication C IDA; HDA; IDA actin cortical patch; nucleus; cytoplasm GO_0030479; GO_0005634; GO_0005737 6632 YPR155C NCA2 Protein that regulates expression of Fo-F1 ATP synthase subunits Protein that regulates expression of Fo-F1 ATP synthase subunits; involved in the regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p C HDA; HDA mitochondrial outer membrane; mitochondrion GO_0005741; GO_0005739 6633 YPR156C TPO3 Polyamine transporter of the major facilitator superfamily Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication C HDA; IMP; IDA cell periphery; fungal-type vacuole membrane; plasma membrane GO_0071944; GO_0000329; GO_0005886 6634 YPR157W TDA6 Putative protein of unknown function Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele; TDA6 has a paralog, VPS62, that arose from the whole genome duplication C HDA; HDA fungal-type vacuole; cell periphery GO_0000324; GO_0071944 6635 YPR158C-C YPR158C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 6636 YPR158C-D YPR158C-D Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 6637 YPR158W CUR1 Sorting factor, central regulator of spatial protein quality control Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication C IDA nucleus GO_0005634 6638 YPR158W-A YPR158W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 6639 YPR158W-B YPR158W-B Retrotransposon TYA Gag and TYB Pol genes Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes C IDA nucleus GO_0005634 6640 YPR159C-A YPR159C-A Protein of unknown function Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching C HDA cytosol GO_0005829 6641 YPR159W KRE6 Type II integral membrane protein Type II integral membrane protein; required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication C IDA; IDA; IDA; IDA; IDA; HDA integral component of endoplasmic reticulum membrane; integral component of membrane; plasma membrane; transport vesicle; site of polarized growth; cellular bud neck GO_0030176; GO_0016021; GO_0005886; GO_0030133; GO_0030427; GO_0005935 6642 YPR160C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6643 YPR160W GPH1 Glycogen phosphorylase required for the mobilization of glycogen Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; expression is regulated by stress-response elements and by the HOG MAP kinase pathway C IDA cytoplasm GO_0005737 6644 YPR160W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry 6645 YPR161C SGV1 Cyclin (Bur2p)-dependent protein kinase Cyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins C IPI; IPI cyclin-dependent protein kinase holoenzyme complex; nucleus GO_0000307; GO_0005634 6646 YPR162C ORC4 Subunit of the origin recognition complex (ORC) Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication C IDA; IDA; IDA DNA replication preinitiation complex; nuclear pre-replicative complex; nuclear origin of replication recognition complex GO_0031261; GO_0005656; GO_0005664 6647 YPR163C TIF3 Translation initiation factor eIF-4B Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel C IDA cytoplasmic stress granule GO_0010494 6648 YPR164W MMS1 Subunit of E3 ubiquitin ligase complex involved in replication repair Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay C IDA Cul8-RING ubiquitin ligase complex GO_0035361 6649 YPR165W RHO1 GTP-binding protein of the rho subfamily of Ras-like proteins GTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) C HDA; IDA; HDA; HDA; IDA; IDA; HDA; IDA; IDA; HDA; HDA; IDA; IDA; IDA endoplasmic reticulum; Golgi apparatus; cellular bud; mitochondrion; peroxisome; cellular bud tip; plasma membrane; cellular bud neck; incipient cellular bud site; mitochondrial outer membrane; cell periphery; 1,3-beta-D-glucan synthase complex; mating projection tip; fungal-type vacuole membrane GO_0005783; GO_0005794; GO_0005933; GO_0005739; GO_0005777; GO_0005934; GO_0005886; GO_0005935; GO_0000131; GO_0005741; GO_0071944; GO_0000148; GO_0043332; GO_0000329 6650 YPR166C MRP2 Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit C HDA; IDA mitochondrion; mitochondrial small ribosomal subunit GO_0005739; GO_0005763 6651 YPR167C MET16 3'-phosphoadenylsulfate reductase 3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism 6652 YPR168W NUT2 Subunit of the RNA polymerase II mediator complex Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress C IDA core mediator complex GO_0070847 6653 YPR169W JIP5 Protein required for biogenesis of the large ribosomal subunit Protein required for biogenesis of the large ribosomal subunit; required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein; essential gene C HDA; HDA nucleolus; nucleus GO_0005730; GO_0005634 6654 YPR169W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps two other dubious ORFs: YPR170C and YPR170W-B 6655 YPR170C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORFs YPR169W-A and YPR170W-B 6656 YPR170W-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry 6657 YPR170W-B YPR170W-B Putative protein of unknown function Putative protein of unknown function; conserved in fungi; partially overlaps the dubious genes YPR169W-A, YPR170W-A and YRP170C 6658 YPR171W BSP1 Adapter that links synaptojanins to the cortical actin cytoskeleton Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p C IDA; HDA; IDA; IDA; IDA actin cortical patch; mating projection tip; membrane; cellular bud neck; cellular bud tip GO_0030479; GO_0043332; GO_0016020; GO_0005935; GO_0005934 6659 YPR172W YPR172W Protein of unknown function Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication C HDA; HDA nucleus; cytoplasm GO_0005634; GO_0005737 6660 YPR173C VPS4 AAA-ATPase involved in multivesicular body (MVB) protein sorting AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism C IDA; IDA; HDA; HDA membrane; endosome; cytoplasm; endoplasmic reticulum GO_0016020; GO_0005768; GO_0005737; GO_0005783 6661 YPR174C YPR174C Protein of unknown function Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication C HDA; IDA; IDA nuclear periphery; nuclear envelope; spindle pole body GO_0034399; GO_0005635; GO_0005816 6662 YPR175W DPB2 Second largest subunit of DNA polymerase II (DNA polymerase epsilon) Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate C IDA; IDA; IDA epsilon DNA polymerase complex; cytoplasm; nucleus GO_0008622; GO_0005737; GO_0005634 6663 YPR176C BET2 Beta subunit of Type II geranylgeranyltransferase Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p C IDA; HDA Rab-protein geranylgeranyltransferase complex; cytosol GO_0005968; GO_0005829 6664 YPR177C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex 6665 YPR178W PRP4 Splicing factor Splicing factor; component of the U4/U6-U5 snRNP complex C IDA U4/U6 x U5 tri-snRNP complex GO_0046540 6666 YPR179C HDA3 Subunit of the HDA1 histone deacetylase complex Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; relocalizes to the cytosol in response to hypoxia; similar to Hda2p C IDA; IDA; HDA HDA1 complex; cytosol; nucleus GO_0070823; GO_0005829; GO_0005634 6667 YPR180W AOS1 Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia C HDA; IDA; IPI nucleus; cytosol; SUMO activating enzyme complex GO_0005634; GO_0005829; GO_0031510 6668 YPR181C SEC23 GTPase-activating protein, stimulates the GTPase activity of Sar1p GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p C IDA; HDA COPII vesicle coat; endoplasmic reticulum GO_0030127; GO_0005783 6669 YPR182W SMX3 Core Sm protein Sm F Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F C IDA; IDA; IDA; IDA U5 snRNP; U4/U6 x U5 tri-snRNP complex; U1 snRNP; U2 snRNP GO_0005682; GO_0046540; GO_0005685; GO_0005686 6670 YPR183W DPM1 Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane; catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation C HDA; IDA; HDA; HDA; HDA mitochondrion; nuclear outer membrane-endoplasmic reticulum membrane network; endoplasmic reticulum; mitochondrial outer membrane; cell periphery GO_0005739; GO_0042175; GO_0005783; GO_0005741; GO_0071944 6671 YPR184W GDB1 Glycogen debranching enzyme Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress C HDA; HDA mitochondrion; cytoplasm GO_0005739; GO_0005737 6672 YPR185W ATG13 Regulatory subunit of the Atg1p signaling complex Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure C IDA; IDA; IDA; HDA NA; extrinsic component of membrane; ATG1/ULK1 kinase complex; cytosol GO_0120095; GO_0019898; GO_1990316; GO_0005829 6673 YPR186C PZF1 Transcription factor IIIA (TFIIIA) Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA 6674 YPR187W RPO26 RNA polymerase subunit ABC23 RNA polymerase subunit ABC23; common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit C IDA; IDA; IDA DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex GO_0005666; GO_0005736; GO_0005665 6675 YPR188C MLC2 Regulatory light chain for the type II myosin Myo1p Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring C IPI; IDA myosin II complex; cellular bud neck contractile ring GO_0016460; GO_0000142 6676 YPR189W SKI3 Ski complex component and TPR protein Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome C IDA Ski complex GO_0055087 6677 YPR190C RPC82 RNA polymerase III subunit C82 RNA polymerase III subunit C82 C IDA DNA-directed RNA polymerase III complex GO_0005666 6678 YPR191W QCR2 Subunit 2 of ubiquinol cytochrome-c reductase (Complex III) Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme C HDA; IDA; IDA mitochondrion; mitochondrial crista; mitochondrial respiratory chain complex III GO_0005739; GO_0030061; GO_0005750 6679 YPR192W AQY1 Spore-specific water channel Spore-specific water channel; mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance C HDA; IDA endoplasmic reticulum; plasma membrane GO_0005783; GO_0005886 6680 YPR193C HPA2 Tetrameric histone acetyltransferase Tetrameric histone acetyltransferase; has similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; also acetylates polyamines C HDA cytoplasm GO_0005737 6681 YPR194C OPT2 Oligopeptide transporter Oligopeptide transporter; localized to peroxisomes and affects glutathione redox homeostasis; also localizes to the plasma membrane (PM) and to the late Golgi, and has a role in maintenance of lipid asymmetry between the inner and outer leaflets of the PM; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles and in polarized cell growth C IDA; IDA; IDA; HDA plasma membrane; peroxisome; Golgi trans cisterna; fungal-type vacuole GO_0005886; GO_0005777; GO_0000138; GO_0000324 6682 YPR195C YPR195C Putative protein of unknown function Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data 6683 YPR196W YPR196W Putative maltose-responsive transcription factor Putative maltose-responsive transcription factor 6684 YPR197C Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SGE1/YPR198W 6685 YPR198W SGE1 Plasma membrane multidrug transporter Plasma membrane multidrug transporter; member of the major facilitator superfamily; acts as an extrusion permease; partial multicopy suppressor of gal11 mutations C IDA; HDA integral component of plasma membrane; endoplasmic reticulum GO_0005887; GO_0005783 6686 YPR199C ARR1 Transcriptional activator of the basic leucine zipper (bZIP) family Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds C IDA; IDA nucleus; cytoplasm GO_0005634; GO_0005737 6687 YPR200C ARR2 Arsenate reductase required for arsenate resistance Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p 6688 YPR201W ARR3 Plasma membrane metalloid/H+ antiporter Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite C IDA plasma membrane GO_0005886 6689 YPR202W YPR202W Putative protein of unknown function Putative protein of unknown function; similar to telomere-encoded helicases; down-regulated at low calcium levels; YPR202W is not an essential gene; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo 6690 YPR203W YPR203W Putative protein of unknown function Putative protein of unknown function 6691 YPR204C-A Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 6692 YPR204W YPR204W DNA helicase encoded within the telomeric Y' element DNA helicase encoded within the telomeric Y' element; Y' -helicase protein 1