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1 Result(s) found for : "(YMR104C)"

#ORFNAMEFUNCTIONDESCRIPTIONGO matched?
1Q0010Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF Q0017
2Q0017Dubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF Q0010
3Q0032Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4Q0045COX1Subunit I of cytochrome c oxidase (Complex IV)Subunit I of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits
5Q0050AI1Reverse transcriptase required for splicing of the COX1 pre-mRNAReverse transcriptase required for splicing of the COX1 pre-mRNA; encoded by a mobile group II intron within the mitochondrial COX1 gene
6Q0055AI2Reverse transcriptase required for splicing of the COX1 pre-mRNAReverse transcriptase required for splicing of the COX1 pre-mRNA; encoded by a mobile group II intron within the mitochondrial COX1 gene
7Q0060AI3Endonuclease I-SceIIIEndonuclease I-SceIII; encoded by a mobile group I intron within the mitochondrial COX1 gene
8Q0065AI4Endonuclease I-SceIIEndonuclease I-SceII; encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity
9Q0070AI5_ALPHAEndonuclease I-SceIVEndonuclease I-SceIV; involved in intron mobility; encoded by a mobile group I intron within the mitochondrial COX1 gene
10Q0075AI5_BETAProtein of unknown functionProtein of unknown function; encoded within an intron of the mitochondrial COX1 gene; translational initiation codon is predicted to be ATA rather than ATG
11Q0080ATP8Subunit 8 of the F0 sector of mitochondrial F1F0 ATP synthaseSubunit 8 of the F0 sector of mitochondrial F1F0 ATP synthase; encoded on the mitochondrial genome; ATP8 and ATP6 mRNAs are not translated in the absence of the F1 sector of ATPase
12Q0085ATP6Subunit a of the F0 sector of mitochondrial F1F0 ATP synthaseSubunit a of the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrially encoded; translation is specifically activated by Atp22p; ATP6 and ATP8 mRNAs are not translated in the absence of the F1 sector of ATPase
13Q0092Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
14Q0105COBCytochrome bCytochrome b; mitochondrially encoded subunit of the ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p
15Q0110BI2Mitochondrial mRNA maturase with a role in splicingMitochondrial mRNA maturase with a role in splicing; encoded by both exon and intron sequences of partially processed COB mRNA
16Q0115BI3Mitochondrial mRNA maturaseMitochondrial mRNA maturase; forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA
17Q0120BI4Mitochondrial mRNA maturaseMitochondrial mRNA maturase; forms a complex with Nam2p to mediate splicing of the bI4 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA
18Q0130OLI1F0-ATP synthase subunit c (ATPase-associated proteolipid)F0-ATP synthase subunit c (ATPase-associated proteolipid); encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2
19Q0140VAR1Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane; may have a role in loss of mitochondrial DNA under stress conditions
20Q0142Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious open reading frame Q0143
21Q0143Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
22Q0144Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
23Q0160SCEII-SceI DNA endonucleaseI-SceI DNA endonuclease; encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene
24Q0182Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
25Q0250COX2Subunit II of cytochrome c oxidase (Complex IV)Subunit II of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits
26Q0255Q0255Maturase-like proteinMaturase-like protein
27Q0275COX3Subunit III of cytochrome c oxidase (Complex IV)Subunit III of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits
28Q0297Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RPM1
29YAL001CTFC3Subunit of RNA polymerase III transcription initiation factor complexSubunit of RNA polymerase III transcription initiation factor complex; part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding; largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC)
30YAL002WVPS8Membrane-binding component of the CORVET complexMembrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
31YAL003WEFB1Translation elongation factor 1 betaTranslation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
32YAL004WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C
33YAL005CSSA1ATPase involved in protein folding and NLS-directed nuclear transportATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
34YAL007CERP2Member of the p24 family involved in ER to Golgi transportMember of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication
35YAL008WFUN14Integral mitochondrial outer membrane (MOM) proteinMitochondrial protein of unknown function
36YAL009WSPO7Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzymePutative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
37YAL010CMDM10Subunit of both the ERMES and the SAM complexSubunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase
38YAL011WSWC3Protein of unknown functionProtein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
39YAL012WCYS3Cystathionine gamma-lyaseCystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
40YAL013WDEP1Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation
41YAL014CSYN8Endosomal SNARE related to mammalian syntaxin 8Endosomal SNARE related to mammalian syntaxin 8
42YAL015CNTG1DNA N-glycosylase and apurinic/apyrimidinic (AP) lyaseDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
43YAL016C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF TPD3/YAL016W
44YAL016C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
45YAL016WTPD3Regulatory subunit A of the heterotrimeric PP2A complexRegulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III
46YAL017WPSK1PAS domain-containing serine/threonine protein kinasePAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
47YAL018CLDS1Protein Involved in spore wall assemblyProtein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants
48YAL019WFUN30Snf2p family member with ATP-dependent chromatin remodeling activitySnf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
49YAL019W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF ATS1/YAL020C
50YAL020CATS1Protein required for modification of wobble nucleosides in tRNAProtein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle
51YAL021CCCR4Component of the CCR4-NOT transcriptional complexComponent of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
52YAL022CFUN26High affinity, broad selectivity, nucleoside/nucleobase transporterHigh affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member
53YAL023CPMT2Protein O-mannosyltransferase of the ER membraneProtein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
54YAL024CLTE1Protein similar to GDP/GTP exchange factorsProtein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
55YAL025CMAK16Essential nuclear proteinEssential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
56YAL026CDRS2Trans-golgi network aminophospholipid translocase (flippase)Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; subject to auto-inhibition by its C-terminal tail; mutations in human homolog ATP8B1 result in liver disease
57YAL026C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAL027W and the verified gene DRS2
58YAL027WSAW15'- and 3'-flap DNA binding protein5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
59YAL028WFRT2Tail-anchored ER membrane protein of unknown functionTail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
60YAL029CMYO4Type V myosin motor involved in actin-based transport of cargosType V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication
61YAL030WSNC1Vesicle membrane receptor protein (v-SNARE)Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
62YAL031CGIP4Cytoplasmic protein that regulates protein phosphatase 1 Glc7pCytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
63YAL031W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF GIP4/YAL031C
64YAL032CPRP45Protein required for pre-mRNA splicingProtein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
65YAL033WPOP5Subunit of both RNase MRP and nuclear RNase PSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
66YAL034CFUN19Non-essential protein of unknown functionNon-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication
67YAL034C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
68YAL034W-AMTW1Essential component of the MIND kinetochore complexEssential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
69YAL035WFUN12Translation initiation factor eIF5BTranslation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
70YAL036CRBG1Member of the DRG family of GTP-binding proteinsMember of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid
71YAL037C-AYAL037C-APutative protein of unknown functionPutative protein of unknown function
72YAL037C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
73YAL037WYAL037WPutative protein of unknown functionPutative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
74YAL038WCDC19Pyruvate kinasePyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
75YAL039CCYC3Cytochrome c heme lyase (holocytochrome c synthase)Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS)
76YAL040CCLN3G1 cyclin involved in cell cycle progressionG1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry.
77YAL041WCDC24Guanine nucleotide exchange factor (GEF) for Cdc42pGuanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
78YAL042C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene
79YAL042WERV46Protein localized to COPII-coated vesiclesProtein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport
80YAL043CPTA1Subunit of holo-CPFSubunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
81YAL044CGCV3H subunit of the mitochondrial glycine decarboxylase complexH subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
82YAL044W-ABOL1Mitochondrial matrix protein involved in Fe-S cluster biogenesisPutative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein
83YAL045CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A
84YAL046CBOL3Protein involved in Fe-S cluster transfer to mitochondrial clientsProtein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss
85YAL047CSPC72Gamma-tubulin small complex (gamma-TuSC) receptorComponent of the cytoplasmic Tub4p (gamma-tubulin) complex; binds spindle pole bodies and links them to microtubules; is regulated by Cdc5 kinase; has roles in astral microtubule formation and stabilization
86YAL047W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF GEM1/YAL048C
87YAL048CGEM1Outer mitochondrial membrane GTPase, subunit of the ERMES complexOuter mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases
88YAL049CAIM2Cytoplasmic protein involved in mitochondrial function or organizationCytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor
89YAL051WOAF1Oleate-activated transcription factorOleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
90YAL053WFLC2Putative calcium channel involved in calcium release under hypotonicPutative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
91YAL054CACS1Acetyl-coA synthetase isoformAcetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
92YAL055WPEX22Putative peroxisomal membrane proteinPutative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation
93YAL056C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
94YAL056WGPB2Multistep regulator of cAMP-PKA signalingMultistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication
95YAL058WCNE1CalnexinCalnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
96YAL059C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM1/YAL059W
97YAL059WECM1Pre-ribosomal factor involved in 60S ribosomal protein subunit exportPre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
98YAL060WBDH1NAD-dependent (R,R)-butanediol dehydrogenaseNAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
99YAL061WBDH2Putative medium-chain alcohol dehydrogenase with similarity to BDH1Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
100YAL062WGDH3NADP(+)-dependent glutamate dehydrogenaseNADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
101YAL063CFLO9Lectin-like protein with similarity to Flo1pLectin-like protein with similarity to Flo1p; thought to be expressed and involved in flocculation
102YAL063C-AYAL063C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
103YAL064C-ATDA8Putative protein of unknown functionPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
104YAL064WYAL064WProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments
105YAL064W-BYAL064W-BFungal-specific protein of unknown functionFungal-specific protein of unknown function
106YAL065CYAL065CPutative protein of unknown functionPutative protein of unknown function; has homology to FLO1; possible pseudogene
107YAL066WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
108YAL067CSEO1Putative permeasePutative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide
109YAL067W-AYAL067W-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
110YAL068CPAU8Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
111YAL068W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
112YAL069WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
113YAR002C-AERP1Member of the p24 family involved in ER to Golgi transportMember of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
114YAR002WNUP60FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153
115YAR003WSWD1Subunit of the COMPASS (Set1C) complexSubunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
116YAR007CRFA1Subunit of heterotrimeric Replication Protein A (RPA)Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia
117YAR008WSEN34Subunit of the tRNA splicing endonucleaseSubunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
118YAR009CYAR009CRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional; protein product forms cytoplasmic foci upon DNA replication stress
119YAR010CYAR010CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; in YARCTY1-1 TYB is mutant and probably non-functional
120YAR014CBUD14Protein involved in bud-site selectionProtein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
121YAR015WADE1N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetaseN-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
122YAR018CKIN3Nonessential serine/threonine protein kinaseNonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol
123YAR019CCDC15Protein kinase of the Mitotic Exit NetworkProtein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
124YAR019W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF CDC15/YAR019C
125YAR020CPAU7Member of the seripauperin multigene familyMember of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
126YAR023CYAR023CPutative integral membrane proteinPutative integral membrane protein; member of DUP240 gene family
127YAR027WUIP3Putative integral membrane protein of unknown functionPutative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
128YAR028WYAR028WPutative integral membrane proteinPutative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
129YAR029WYAR029WMember of DUP240 gene family but contains no transmembrane domainsMember of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
130YAR030CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9
131YAR031WPRM9Pheromone-regulated proteinPheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication
132YAR033WMST28Putative integral membrane protein, involved in vesicle formationPutative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles; MST28 has a paralog, MST27, that arose from a segmental duplication
133YAR035C-AYAR035C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis
134YAR035WYAT1Outer mitochondrial carnitine acetyltransferaseOuter mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
135YAR042WSWH1Protein similar to mammalian oxysterol-binding proteinProtein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction; SWH1 has a paralog, OSH2, that arose from the whole genome duplication
136YAR047CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
137YAR050WFLO1Lectin-like protein involved in flocculationLectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; FLO1 has a paralog, FLO5, that arose from a segmental duplication
138YAR053WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
139YAR060CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YAR060C has a paralog, YHR212C, that arose from a segmental duplication
140YAR064WYAR064WPseudogenic fragment with similarity to flocculinsPseudogenic fragment with similarity to flocculins; YAR064W has a paralog, YHR213W-B, that arose from a segmental duplication
141YAR066WYAR066WPutative GPI proteinPutative GPI protein; YAR066W has a paralog, YHR214W, that arose from a segmental duplication
142YAR068WYAR068WFungal-specific protein of unknown functionFungal-specific protein of unknown function; induced in respiratory-deficient cells; YAR068W has a paralog, YHR214W-A, that arose from a segmental duplication
143YAR069CDubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; YAR069C has a paralog, YHR214C-D, that arose from a segmental duplication
144YAR070CDubious open reading frame; unlikely to encode a protein, based on available experimental and comparative sequence data; YAR070C has a paralog, YHR214C-B, that arose from a segmental duplication
145YAR071WPHO11One of three repressible acid phosphatasesOne of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2; PHO11 has a paralog, PHO12, that arose from a segmental duplication
146YAR073WNonfunctional protein with homology to IMP dehydrogenase; blocked reading frame, located close to the telomere; not expressed at detectable levels; YAR073W and YAR075W comprise a continuous reading frame in most strains of S. cerevisiae; YAR073W/YAR075W together have a paralog, IMD2, that arose from a segmental duplication
147YAR075WNon-functional protein with homology IMP dehydrogenase; YAR073W/IMD1 and YAR075W comprise a continuous reading frame in most strains of S. cerevisiae; YAR073W/YAR075W together have a paralog, IMD2, that arose from a segmental duplication
148YBL001CECM15Non-essential protein of unknown functionNon-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
149YBL002WHTB2Histone H2BHistone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
150YBL003CHTA2Histone H2AHistone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
151YBL004WUTP20Component of the small-subunit (SSU) processomeComponent of the small-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA
152YBL005WPDR3Transcriptional activator of the pleiotropic drug resistance networkTranscriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate; PDR3 has a paralog, PDR1, that arose from the whole genome duplication
153YBL005W-AYBL005W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
154YBL005W-BYBL005W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
155YBL006CLDB7Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
156YBL006W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene LDB7/YBL006C
157YBL007CSLA1Cytoskeletal protein binding proteinCytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
158YBL008WHIR1Subunit of the HIR complexSubunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
159YBL008W-AYBL008W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
160YBL009WALK2Protein kinaseProtein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins
161YBL010CYBL010CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
162YBL011WSCT1Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferaseGlycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed
163YBL012CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
164YBL013WFMT1Methionyl-tRNA formyltransferaseMethionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
165YBL014CRRN6Component of the core factor (CF) rDNA transcription factor complexComponent of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
166YBL015WACH1Protein with CoA transferase activityProtein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
167YBL016WFUS3Mitogen-activated serine/threonine protein kinase involved in matingMitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
168YBL017CPEP1Type I transmembrane sorting receptor for multiple vacuolar hydrolasesType I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
169YBL018CPOP8Subunit of both RNase MRP and nuclear RNase PSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
170YBL019WAPN2Class II abasic (AP) endonuclease involved in repair of DNA damageClass II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
171YBL020WRFT1Membrane protein required for translocation of Man5GlcNac2-PP-DolMembrane protein required for translocation of Man5GlcNac2-PP-Dol; required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein; essential gene
172YBL021CHAP3Subunit of the Hap2p/3p/4p/5p CCAAT-binding complexSubunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
173YBL022CPIM1ATP-dependent Lon proteaseATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
174YBL023CMCM2Protein involved in DNA replicationProtein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
175YBL024WNCL1S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferaseS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
176YBL025WRRN10Protein involved in promoting high level transcription of rDNAProtein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
177YBL026WLSM2Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
178YBL027WRPL19BRibosomal 60S subunit protein L19BRibosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
179YBL028CYBL028CProtein of unknown function that may interact with ribosomesProtein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
180YBL029C-AYBL029C-AProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
181YBL029WYBL029WNon-essential protein of unknown functionNon-essential protein of unknown function
182YBL030CPET9Major ADP/ATP carrier of the mitochondrial inner membraneMajor ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
183YBL031WSHE1Mitotic spindle proteinMitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
184YBL032WHEK2RNA binding protein involved in asymmetric localization of ASH1 mRNARNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
185YBL033CRIB1GTP cyclohydrolase IIGTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway
186YBL034CSTU1Component of the mitotic spindleComponent of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
187YBL035CPOL12B subunit of DNA polymerase alpha-primase complexB subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
188YBL036CYBL036CPutative non-specific single-domain racemasePutative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
189YBL037WAPL3Alpha-adaptinAlpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
190YBL038WMRPL16Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetically lethal with hac1 mutant suggesting a role in the maturation of secretory proteins
191YBL039CURA7Major CTP synthase isozyme (see also URA8)Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication
192YBL039C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF URA7; identified by expression profiling and mass spectrometry
193YBL039W-BMIN6Putative protein of unknown functionPutative protein of unknown function
194YBL040CERD2HDEL receptorHDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
195YBL041WPRE7Beta 6 subunit of the 20S proteasomeBeta 6 subunit of the 20S proteasome
196YBL042CFUI1High affinity uridine permease, localizes to the plasma membraneHigh affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
197YBL043WECM13Non-essential protein of unknown functionNon-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication
198YBL044WYBL044WPutative protein of unknown functionPutative protein of unknown function; YBL044W is not an essential protein
199YBL045CCOR1Core subunit of the ubiquinol-cytochrome c reductase complexCore subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain
200YBL046WPSY4Regulatory subunit of protein phosphatase PP4Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
201YBL047CEDE1Scaffold protein involved in the formation of early endocytic sitesPutative regulator of cytokinesis; also involved in endocytosis and has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death
202YBL048WRRT1Putative protein of unknown functionProtein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
203YBL049WMOH1Protein of unknown function, essential for stationary phase survivalProtein of unknown function; has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase; possibly linked with vacuolar transport
204YBL050WSEC17Alpha-SNAP cochaperoneAlpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP
205YBL051CPIN4Protein involved in G2/M phase progression and response to DNA damageProtein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
206YBL052CSAS3Histone acetyltransferase catalytic subunit of NuA3 complexHistone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
207YBL053WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
208YBL054WTOD6PAC motif binding protein involved in rRNA and ribosome biogenesisPAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization
209YBL055CYBL055C3'-->5' exonuclease and endonuclease with a possible role in apoptosis3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
210YBL056WPTC3Type 2C protein phosphatase (PP2C)Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
211YBL057CPTH2One of two mitochondrially-localized peptidyl-tRNA hydrolasesOne of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1
212YBL058WSHP1UBX domain-containing substrate adaptor for Cdc48pUBX (ubiquitin regulatory X) domain-containing protein; regulates Glc7p phosphatase activity; shp1 mutants are impaired in growth and mitotic progression; functions in growth and mitotic progression require Cdc48p binding; mitotic phenotype is caused by reduced Glc7p activity; interacts with ubiquitylated proteins, required for degradation of a ubiquitylated model substrate
213YBL059C-ACMC2Protein involved in respiratory chain complex assembly or maintenanceProtein involved in respiratory chain complex assembly or maintenance; protein of the mitochondrial intermembrane space; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold
214YBL059WIAI11Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
215YBL060WYEL1Guanine nucleotide exchange factor specific for Arf3pGuanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
216YBL061CSKT5Activator of Chs3p (chitin synthase III) during vegetative growthActivator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
217YBL062WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
218YBL063WKIP1Kinesin-related motor proteinKinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
219YBL064CPRX1Mitochondrial peroxiredoxin with thioredoxin peroxidase activityMitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
220YBL065WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
221YBL066CSEF1Putative transcription factorPutative transcription factor; has homolog in Kluyveromyces lactis
222YBL067CUBP13Ubiquitin-specific protease that cleaves Ub-protein fusionsUbiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
223YBL068WPRS45-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic
224YBL068W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by fungal homology and RT-PCR
225YBL069WAST1Lipid raft associated proteinLipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
226YBL070CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
227YBL071CYBL071CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
228YBL071C-BYBL071C-BPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
229YBL071W-AKTI11Zn-ribbon protein that co-purifies with Dph1 and Dph2Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs
230YBL072CRPS8AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication
231YBL073WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
232YBL074CAAR2Component of the U5 snRNP complexComponent of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
233YBL075CSSA3ATPase involved in protein folding and the response to stressATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
234YBL076CILS1Cytoplasmic isoleucine-tRNA synthetaseCytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A
235YBL077WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
236YBL078CATG8Component of autophagosomes and Cvt vesiclesComponent of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
237YBL079WNUP170Subunit of inner ring of nuclear pore complex (NPC)Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
238YBL080CPET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complexSubunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
239YBL081WYBL081WNon-essential protein of unknown functionNon-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport
240YBL082CALG3Dolichol-P-Man dependent alpha(1-3) mannosyltransferaseDolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
241YBL083CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
242YBL084CCDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
243YBL085WBOI1Protein implicated in polar growthProtein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
244YBL086CYBL086CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
245YBL087CRPL23ARibosomal 60S subunit protein L23ARibosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
246YBL088CTEL1Protein kinase primarily involved in telomere length regulationProtein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
247YBL089WAVT5Putative transporterPutative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
248YBL090WMRP21Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
249YBL091CMAP2Methionine aminopeptidaseMethionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
250YBL091C-ASCS22Protein involved in regulation of phospholipid metabolismProtein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication
251YBL092WRPL32Ribosomal 60S subunit protein L32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
252YBL093CROX3Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
253YBL094CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
254YBL095WMRX3Protein that associates with mitochondrial ribosomePutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
255YBL096CNon-essential protein of unknown function
256YBL097WBRN1Subunit of the condensin complexSubunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
257YBL098WBNA4Kynurenine 3-monooxygenaseKynurenine 3-mono oxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
258YBL099WATP1Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthaseAlpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo
259YBL100CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1
260YBL100W-AYBL100W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
261YBL100W-BYBL100W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
262YBL100W-CYBL100W-CPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
263YBL101CECM21Protein involved in regulating endocytosis of plasma membrane proteinsProtein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
264YBL102WSFT2Tetra-spanning membrane protein found mostly in the late GolgiTetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
265YBL103CRTG3bHLH/Zip transcription factor for retrograde (RTG) and TOR pathwaysbHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
266YBL104CSEA4Subunit of SEACAT, a subcomplex of the SEA complexSubunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
267YBL105CPKC1Protein serine/threonine kinaseProtein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
268YBL106CSRO77Protein with roles in exocytosis and cation homeostasisProtein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
269YBL107CMIX23Mitochondrial intermembrane space protein of unknown functionMitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
270YBL107W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the Ty2 LTR YBLWdelta2; YBL107W-A has a paralog, YER138W-A, that arose from a single-locus duplication
271YBL108C-APAU9Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
272YBL108WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
273YBL109WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
274YBL111CYBL111CHelicase-like protein encoded within the telomeric Y' elementHelicase-like protein encoded within the telomeric Y' element; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
275YBL112CYBL112CPutative protein of unknown functionPutative protein of unknown function; YBL112C is contained within TEL02L
276YBL113CYBL113CHelicase-like protein encoded within the telomeric Y' elementHelicase-like protein encoded within the telomeric Y' element
277YBL113W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
278YBR001CNTH2Putative neutral trehalase, required for thermotolerancePutative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
279YBR002CRER2Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1Cis-prenyltransferase involved in dolichol synthesis; major enzyme of polyprenol synthesis in both the endoplasmic reticulum (ER) and in lipid droplets; participates in ER protein sorting
280YBR003WCOQ1Hexaprenyl pyrophosphate synthetaseHexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
281YBR004CGPI18Functional ortholog of human PIG-VFunctional ortholog of human PIG-V; PIG-V is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
282YBR005WRCR1Protein of the ER membrane involved in cell wall chitin depositionProtein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication
283YBR006WUGA2Succinate semialdehyde dehydrogenaseSuccinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
284YBR007CDSF2Deletion suppressor of mpt5 mutationDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
285YBR008CFLR1Plasma membrane transporter of the major facilitator superfamilyPlasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
286YBR009CHHF1Histone H4Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
287YBR010WHHT1Histone H3Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
288YBR011CIPP1Cytoplasmic inorganic pyrophosphatase (PPase)Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
289YBR012CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by iron-regulated transcriptional activator Aft2p
290YBR012W-AYBR012W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
291YBR012W-BYBR012W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
292YBR013CYBR013CPutative protein of unknown functionPutative protein of unknown function; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
293YBR014CGRX7Cis-golgi localized monothiol glutaredoxinCis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
294YBR015CMNN2Alpha-1,2-mannosyltransferaseAlpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
295YBR016WYBR016WTail-anchored plasma membrane protein with a conserved CYSTM moduleTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
296YBR017CKAP104Transportin or cytosolic karyopherin beta 2Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis
297YBR018CGAL7Galactose-1-phosphate uridyl transferaseGalactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
298YBR019CGAL10UDP-glucose-4-epimeraseUDP-glucose-4-epimerase; catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers
299YBR020WGAL1GalactokinaseGalactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
300YBR021WFUR4Plasma membrane localized uracil permeasePlasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
301YBR022WPOA1Phosphatase that is highly specific for ADP-ribose 1''-phosphatePhosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
302YBR023CCHS3Chitin synthase IIIChitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention
303YBR024WSCO2Protein anchored to mitochondrial inner membraneProtein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
304YBR025COLA1P-loop ATPase with similarity to human OLA1 and bacterial YchFP-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
305YBR026CETR12-enoyl thioester reductase2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
306YBR027CYBR027CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
307YBR028CYPK3AGC kinaseAGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner
308YBR029CCDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase)Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
309YBR030WRKM3Ribosomal lysine methyltransferaseRibosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia
310YBR031WRPL4ARibosomal 60S subunit protein L4ARibosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication
311YBR032WYBR032WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
312YBR033WEDS1Putative zinc cluster protein, predicted to be a transcription factorPutative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication
313YBR034CHMT1Nuclear SAM-dependent mono- and asymmetric methyltransferaseNuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.
314YBR035CPDX3Pyridoxine (pyridoxamine) phosphate oxidasePyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
315YBR036CCSG2Endoplasmic reticulum membrane proteinEndoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
316YBR037CSCO1Copper-binding protein of mitochondrial inner membraneCopper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication
317YBR038WCHS2Chitin synthase IIChitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
318YBR039WATP3Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthaseGamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
319YBR040WFIG1Integral membrane protein required for efficient matingIntegral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating
320YBR041WFAT1Very long chain fatty acyl-CoA synthetase and fatty acid transporterVery long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
321YBR042CCST26AcyltransferasePutative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
322YBR043CQDR3Multidrug transporter of the major facilitator superfamilyMultidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
323YBR044CTCM62Protein involved in assembly of the succinate dehydrogenase complexProtein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone
324YBR045CGIP1Meiosis-specific regulatory subunit of the Glc7p protein phosphataseMeiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p
325YBR046CZTA1NADPH-dependent quinone reductaseNADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
326YBR047WFMP23Putative protein of unknown functionPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
327YBR048WRPS11BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
328YBR049CREB1RNA polymerase I enhancer binding proteinRNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
329YBR050CREG2Regulatory subunit of the Glc7p type-1 protein phosphataseRegulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication
330YBR051WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase
331YBR052CRFS1Protein of unknown functionProtein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
332YBR053CYBR053CPutative protein of unknown functionPutative protein of unknown function; induced by cell wall perturbation
333YBR054WYRO2Protein with a putative role in response to acid stressProtein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
334YBR055CPRP6Splicing factorSplicing factor; component of the U4/U6-U5 snRNP complex
335YBR056C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the dubious ORF YBR056W-A
336YBR056WYBR056WPutative glycoside hydrolase of the mitochondrial intermembrane spacePutative glycoside hydrolase of the mitochondrial intermembrane space
337YBR056W-AMNC1Protein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps dubious ORF YBR056C-B; YBR056W-A has a paralog, YDR034W-B, that arose from the whole genome duplication
338YBR057CMUM2Protein essential for meiotic DNA replication and sporulationProtein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
339YBR058CUBP14Ubiquitin-specific proteaseUbiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
340YBR058C-ATSC3Protein that stimulates the activity of serine palmitoyltransferaseProtein that stimulates the activity of serine palmitoyltransferase; involved in sphingolipid biosynthesis; Lcb1p and Lcb2p are the two components of serine palmitoyltransferase
341YBR059CAKL1Ser-Thr protein kinaseSer-Thr protein kinase; member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization
342YBR060CORC2Subunit of the origin recognition complex (ORC)Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
343YBR061CTRM72'-O-ribose methyltransferase2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
344YBR062CYBR062CProtein of unknown function that interacts with Msb2pProtein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway
345YBR063CYBR063CPutative protein of unknown functionPutative protein of unknown function; YBR063C is not an essential gene
346YBR064WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR063C
347YBR065CECM2Pre-mRNA splicing factorPre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
348YBR066CNRG2Transcriptional repressorTranscriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose
349YBR067CTIP1Major cell wall mannoprotein with possible lipase activityMajor cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
350YBR068CBAP2High-affinity leucine permeaseHigh-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
351YBR069CTAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosineAmino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
352YBR070CALG14Component of UDP-GlcNAc transferaseComponent of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
353YBR071WYBR071WProtein of unknown function found in the cytoplasm and bud neckProtein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
354YBR072C-AYBR072C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
355YBR072WHSP26Small heat shock protein (sHSP) with chaperone activitySmall heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
356YBR073WRDH54DNA-dependent ATPaseDNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
357YBR074WPFF1Multi-spanning vacuolar membrane proteaseMulti-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to the M28 family of metalloproteases; has a lumenal-facing protease domain; proposed role in vacuole physiology
358YBR076C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM8; identified by fungal homology and RT-PCR
359YBR076WECM8Non-essential protein of unknown functionNon-essential protein of unknown function
360YBR077CSLM4Subunit of the EGO/GSE complexComponent of the EGO and GSE complexes; essential for integrity and function of EGO; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
361YBR078WECM33GPI-anchored protein of unknown functionGPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication
362YBR079CRPG1eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3)eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
363YBR080CSEC18AAA ATPase and SNARE disassembly chaperoneAAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
364YBR081CSPT7Subunit of the SAGA transcriptional regulatory complexSubunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
365YBR082CUBC4Ubiquitin-conjugating enzyme (E2)Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
366YBR083WTEC1Transcription factor targeting filamentation genes and Ty1 expressionTranscription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
367YBR084C-ARPL19ARibosomal 60S subunit protein L19ARibosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
368YBR084WMIS1Mitochondrial C1-tetrahydrofolate synthaseMitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
369YBR085C-AYBR085C-AProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
370YBR085WAAC3Mitochondrial inner membrane ADP/ATP translocatorMitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
371YBR086CIST2Cortical ER protein involved in ER-plasma membrane tetheringCortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
372YBR087WRFC5Subunit of heteropentameric Replication factor C (RF-C)Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
373YBR088CPOL30Proliferating cell nuclear antigen (PCNA)Proliferating cell nuclear antigen (PCNA); functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
374YBR089C-ANHP6BHigh-mobility group (HMG) proteinHigh-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
375YBR089WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
376YBR090CYBR090CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
377YBR091CTIM12Essential protein of the inner mitochondrial membraneEssential protein of the inner mitochondrial membrane; peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
378YBR092CPHO3Constitutively expressed acid phosphatase similar to Pho5pConstitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
379YBR093CPHO5Repressible acid phosphataseRepressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
380YBR094WPBY1Putative tubulin tyrosine ligase associated with P-bodiesPutative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress
381YBR095CRXT2Component of the histone deacetylase Rpd3L complexComponent of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia
382YBR096WYBR096WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER
383YBR097WVPS15Serine/threonine protein kinase involved in vacuolar protein sortingSerine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
384YBR098WMMS4Subunit of structure-specific Mms4p-Mus81p endonucleaseSubunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p
385YBR099CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
386YBR101CFES1Hsp70 (Ssa1p) nucleotide exchange factorHsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
387YBR102CEXO84Exocyst subunit with dual roles in exocytosis and spliceosome assemblyExocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
388YBR103C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by homology to related yeast
389YBR103WSIF2WD40 repeat-containing subunit of Set3C histone deacetylase complexWD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
390YBR104WYMC2Putative mitochondrial inner membrane transporterPutative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
391YBR105CVID24GID Complex regulatory subunitGID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
392YBR106WSND3Protein involved in SRP-independent targeting of substrates to the ERProbable membrane protein; involved in phosphate transport; role in the maturation of secretory proteins; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
393YBR107CIML3Outer kinetochore protein and component of the Ctf19 complexOuter kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
394YBR108WAIM3Protein that inhibits barbed-end actin filament elongationProtein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
395YBR109CCMD1CalmodulinCalmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
396YBR109W-APutative protein of unknown function; questionable ORF from MIPS
397YBR110WALG1MannosyltransferaseMannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
398YBR111CYSA1Nudix hydrolase family member with ADP-ribose pyrophosphatase activityNudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate
399YBR111W-ASUS1Component of both the SAGA histone acetylase and TREX-2 complexesComponent of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
400YBR112CCYC8General transcriptional co-repressorGeneral transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+]
401YBR113WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8
402YBR114WRAD16Nucleotide excision repair (NER) proteinNucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
403YBR115CLYS2Alpha aminoadipate reductaseAlpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p
404YBR116CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2
405YBR117CTKL2TransketolaseTransketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication
406YBR118WTEF2Translational elongation factor EF-1 alphaTranslational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; TEF2-RFP levels increase during replicative aging; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
407YBR119WMUD1U1 snRNP A proteinU1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing
408YBR120CCBP6Mitochondrial protein required for translation of the COB mRNAMitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
409YBR121CGRS1Cytoplasmic and mitochondrial glycyl-tRNA synthaseCytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
410YBR121C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely contained within the verified gene GRS1; identified by expression profiling and mass spectrometry
411YBR122CMRPL36Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region
412YBR123CTFC1Subunit of RNA polymerase III transcription initiation factor complexSubunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
413YBR124WPutative protein of unknown function
414YBR125CPTC4Cytoplasmic type 2C protein phosphatase (PP2C)Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
415YBR126CTPS1Synthase subunit of trehalose-6-P synthase/phosphatase complexSynthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
416YBR126W-AMEO1Putative protein of unknown functionProtein of unknown function; identified by gene-trapping, microarray analysis, and genome-wide homology searches; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR126W-B
417YBR126W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YBR126W-A; identified by SAGE
418YBR127CVMA2Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPaseSubunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress
419YBR128CATG14Autophagy-specific subunit of phosphatidylinositol 3-kinase complex IAutophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p
420YBR129COPY1Protein of unknown functionProtein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
421YBR130CSHE3Protein adaptor between Myo4p and the She2p-mRNA complexProtein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance
422YBR131C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR131W; identified by gene-trapping, microarray analysis, and genome-wide homology searches
423YBR131WCCZ1Subunit of a heterodimeric guanine nucleotide exchange factor (GEF)Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy
424YBR132CAGP2Plasma membrane regulator of polyamine and carnitine transportPlasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes
425YBR133CHSL7Protein arginine N-methyltransferaseProtein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
426YBR134WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
427YBR135WCKS1Cyclin-dependent protein kinase regulatory subunit and adaptorCyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p(Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates the phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
428YBR136WMEC1Genome integrity checkpoint protein and PI kinase superfamily memberGenome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres
429YBR137WYBR137WProtein with a role in ER delivery of tail-anchored membrane proteinsProtein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene
430YBR138CYBR138CCytoplasmic protein of unknown functionCytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
431YBR139WATG42Vacuolar serine-type carboxypeptidasePutative serine type carboxypeptidase; role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
432YBR140CIRA1GTPase-activating proteinGTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
433YBR141CBMT2Nucleolar S-adenosylmethionine-dependent rRNA methyltransferaseNucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
434YBR141W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YBR141C; identified by gene-trapping, microarray analysis, and genome-wide homology searching
435YBR142WMAK5Essential nucleolar proteinEssential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
436YBR143CSUP45Polypeptide release factor (eRF1) in translation terminationPolypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
437YBR144CYBR144CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
438YBR145WADH5Alcohol dehydrogenase isoenzyme VAlcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
439YBR146WMRPS9Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
440YBR147WRTC2Putative vacuolar membrane transporter for cationic amino acidsPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication
441YBR148WYSW1Protein required for normal prospore membrane formationProtein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication
442YBR149WARA1NADP+ dependent arabinose dehydrogenaseNADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
443YBR150CTBS1Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
444YBR151WAPD1Protein of unknown functionProtein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
445YBR152WSPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNPmRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia
446YBR153WRIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminaseDiaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
447YBR154CRPB5RNA polymerase subunit ABC27RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
448YBR155WCNS1TPR-containing co-chaperoneTPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
449YBR156CSLI15Subunit of the conserved chromosomal passenger complex (CPC)Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
450YBR157CICS2Protein of unknown functionProtein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization
451YBR158WAMN1Protein required for daughter cell separationProtein required for daughter cell separation; multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)
452YBR159WIFA38Microsomal beta-keto-reductaseMicrosomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
453YBR160WCDC28Cyclin-dependent kinase (CDK) catalytic subunitCyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1 (CLNs), S and G2/M (CLBs) phase cyclins, which provide substrate specificity; regulates cell cycle and basal transcription, chromosome duplication and segregation, lipid biosynthesis, membrane trafficking, polarized growth, and morphogenesis; abundance increases in DNA replication stress; transcript induction in osmostress involves antisense RNA
454YBR161WCSH1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunitMannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
455YBR162CTOS1Covalently-bound cell wall protein of unknown functionCovalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
456YBR162W-AYSY6Protein of unknown functionProtein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
457YBR163WEXO5Mitochondrial 5'-3' exonuclease and sliding exonucleaseMitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2
458YBR164CARL1Soluble GTPase with a role in regulation of membrane trafficSoluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; G protein of the Ras superfamily, similar to ADP-ribosylation factor
459YBR165WUBS1Ubiquitin-conjugating enzyme suppressor that regulates Cdc34pUbiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
460YBR166CTYR1Prephenate dehydrogenase involved in tyrosine biosynthesisPrephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels
461YBR167CPOP7Subunit of RNase MRP, nuclear RNase P and telomeraseSubunit of both RNase MRP and nuclear RNase P; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
462YBR168WPEX32Peroxisomal integral membrane proteinPeroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
463YBR169CSSE2Member of Hsp110 subclass of the heat shock protein 70 (HSP70) familyMember of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
464YBR170CNPL4Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregaseSubstrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
465YBR171WSEC66Non-essential subunit of Sec63 complexNon-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec72p
466YBR172CSMY2GYF domain proteinGYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
467YBR173CUMP1Chaperone required for correct maturation of the 20S proteasomeChaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
468YBR174CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
469YBR175WSWD3Essential subunit of the COMPASS (Set1C) complexEssential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
470YBR176WECM31Ketopantoate hydroxymethyltransferaseKetopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
471YBR177CEHT1Acyl-coenzymeA:ethanol O-acyltransferaseAcyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication
472YBR178WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C
473YBR179CFZO1MitofusinMitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
474YBR180WDTR1Putative dityrosine transporter of the major facilitator superfamilyPutative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation
475YBR181CRPS6BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
476YBR182CSMP1MADS-box transcription factor involved in osmotic stress responseMADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
477YBR182C-AYBR182C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
478YBR183WYPC1Alkaline ceramidaseAlkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication
479YBR184WYBR184WPutative protein of unknown functionPutative protein of unknown function; YBR184W is not an essential gene
480YBR185CMBA1Membrane-associated mitochondrial ribosome receptorMembrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane
481YBR186WPCH2Hexameric ring ATPase that remodels chromosome axis protein Hop1pHexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation
482YBR187WGDT1Calcium and manganese transporter with higher affinity for Ca2+Protein of unknown function involved in calcium homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion protein localizes to the vacuole; TMEM165, a human gene which causes Congenital Disorders of Glycosylation is orthologous and functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression reduced in a gcr1 null mutant
483YBR188CNTC20Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
484YBR189WRPS9BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
485YBR190WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
486YBR191WRPL21ARibosomal 60S subunit protein L21ARibosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
487YBR191W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
488YBR192WRIM2Mitochondrial pyrimidine nucleotide transporterMitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
489YBR193CMED8Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
490YBR194WAIM4Protein proposed to be associated with the nuclear pore complexProtein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
491YBR195CMSI1Subunit of chromatin assembly factor I (CAF-1)Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase
492YBR196CPGI1Glycolytic enzyme phosphoglucose isomeraseGlycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
493YBR196C-AYBR196C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
494YBR196C-BYBR196C-BPutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
495YBR197CYBR197CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
496YBR198CTAF5Subunit (90 kDa) of TFIID and SAGA complexesSubunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
497YBR199WKTR4Glycosyltransferase involved in protein glycosylationPutative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain
498YBR200WBEM1Protein containing SH3-domainsProtein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
499YBR200W-AYBR200W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
500YBR201C-AMIN7Putative protein of unknown functionPutative protein of unknown function
501YBR201WDER1ER membrane protein that promotes export of misfolded polypeptidesER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into the ER membrane and routing them to Hrd1p for ubiquitylation; N- and C- termini protrude into the cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1
502YBR202WMCM7Component of the Mcm2-7 hexameric helicase complexComponent of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
503YBR203WCOS111Protein required for antifungal drug ciclopirox olamine resistanceProtein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
504YBR204CLDH1Serine hydrolaseSerine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
505YBR205WKTR3Putative alpha-1,2-mannosyltransferasePutative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress
506YBR206WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3
507YBR207WFTH1Putative high affinity iron transporterPutative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
508YBR208CDUR1,2Urea amidolyaseUrea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress
509YBR209WYBR209WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene
510YBR210WERV15Protein involved in export of proteins from the endoplasmic reticulumProtein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication
511YBR211CAME1Essential kinetochore protein associated with microtubules and SPBsEssential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
512YBR212WNGR1RNA binding protein that negatively regulates growth rateRNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
513YBR213WMET8Bifunctional dehydrogenase and ferrochelataseBifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
514YBR214WSDS24Protein involved in cell separation during buddingProtein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
515YBR215WHPC2Subunit of the HIR complexSubunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
516YBR216CYBP1Protein involved in cellular response to oxidative stressProtein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
517YBR217WATG12Ubiquitin-like modifier involved in autophagy and the Cvt pathwayUbiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p cojugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation
518YBR218CPYC2Pyruvate carboxylase isoformPyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
519YBR219CYBR219CPutative protein of unknown functionPutative protein of unknown function; YBR219C is not an essential gene
520YBR220CYBR220CPutative protein of unknown functionPutative protein of unknown function; YBR220C is not an essential gene
521YBR221CPDB1E1 beta subunit of the pyruvate dehydrogenase (PDH) complexE1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria
522YBR221W-AYBR221W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
523YBR222CPCS60Oxalyl-CoA synthetaseOxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase
524YBR223CTDP1Tyrosyl-DNA phosphodiesterase ITyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI
525YBR223W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps YBR223C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
526YBR224WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1
527YBR225WYBR225WPutative protein of unknown functionPutative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
528YBR226CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
529YBR227CMCX1Non-proteolytic ATPase of the AAA familyMitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins
530YBR228WSLX1Endonuclease involved in DNA recombination and repairEndonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
531YBR229CROT2Glucosidase II catalytic subunitGlucosidase II catalytic subunit; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
532YBR230COM14Mitochondrial outer membrane receptor for cytosolic ribosomesIntegral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron
533YBR230W-ACOQ21Putative protein of unknown functionPutative protein of unknown function; YBR230W-A has a paralog, COQ8, that arose from the whole genome duplication
534YBR231CSWC5Component of the SWR1 complexComponent of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
535YBR232CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
536YBR233WPBP2RNA binding proteinRNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
537YBR233W-ADAD3Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
538YBR234CARC40Subunit of the ARP2/3 complexSubunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
539YBR235WVHC1Vacuolar membrane cation-chloride cotransporter (CCC)Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
540YBR236CABD1MethyltransferaseMethyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia
541YBR237WPRP5RNA helicase in the DEAD-box familyRNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
542YBR238CYBR238CMitochondrial membrane proteinMitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication
543YBR239CERT1Transcriptional regulatorTranscriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p
544YBR240CTHI2Transcriptional activator of thiamine biosynthetic genesTranscriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
545YBR241CYBR241CPutative transporter, member of the sugar porter familyPutative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication
546YBR242WYBR242WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication
547YBR243CALG7UDP-N-acetyl-glucosamine-1-P transferaseUDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
548YBR244WGPX2Phospholipid hydroperoxide glutathione peroxidasePhospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
549YBR245CISW1ATPase subunit of imitation-switch (ISWI) class chromatin remodelersATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK
550YBR246WRRT2Methylesterase performing penultimate step of diphthamide biosynthesisMethylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins
551YBR247CENP1Protein associated with U3 and U14 snoRNAsProtein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
552YBR248CHIS7Imidazole glycerol phosphate synthaseImidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
553YBR249CARO43-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
554YBR250WSPO23Protein of unknown functionProtein of unknown function; associates with meiosis-specific protein Spo1p
555YBR251WMRPS5Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
556YBR252WDUT1Deoxyuridine triphosphate diphosphatase (dUTPase)deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
557YBR253WSRB6Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
558YBR254CTRS20Core component of transport protein particle (TRAPP) complexes I-IIICore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation of conserved residues leads to defects in endocytic recycling and a block in sporulation/meiosis; mutations in the human homolog cause the spondyloepiphyseal dysplasia
559YBR255C-ARCF3Putative protein of unknown functionPutative protein of unknown function; may interact with respiratory chain complexes III (ubiquinol-cytochrome c reductase) or IV (cytochrome c oxidase); identified by sequence comparison with hemiascomycetous yeast species
560YBR255WMTC4Protein of unknown functionProtein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
561YBR256CRIB5Riboflavin synthaseRiboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
562YBR257WPOP4Subunit of both RNase MRP and nuclear RNase PSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
563YBR258CSHG1Subunit of the COMPASS (Set1C) complexSubunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
564YBR259WYBR259WProtein of unknown functionProtein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
565YBR260CRGD1GTPase-activating protein (RhoGAP) for Rho3p and Rho4pGTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization
566YBR261CTAE1AdoMet-dependent proline methyltransferaseAdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
567YBR262CMIC12Component of the MICOS complexComponent of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane
568YBR263WSHM1Mitochondrial serine hydroxymethyltransferaseMitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
569YBR264CYPT10Rab family GTP-binding proteinRab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
570YBR265WTSC103-ketosphinganine reductase3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family
571YBR266CProtein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
572YBR267WREI1Cytoplasmic pre-60S factorCytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
573YBR268WMRPL37Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
574YBR269CSDH8Protein required for assembly of succinate dehydrogenaseProtein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs
575YBR270CBIT2Subunit of TORC2 membrane-associated complexSubunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication
576YBR271WEFM2S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis
577YBR272CHSM3Evolutionarily conserved 19S regulatory particle assembly-chaperoneProteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation
578YBR273CUBX7UBX (ubiquitin regulatory X) domain-containing proteinUBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
579YBR274WCHK1Serine/threonine kinase and DNA damage checkpoint effectorSerine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
580YBR275CRIF1Protein that binds to the Rap1p C-terminusProtein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation
581YBR276CPPS1Protein phosphataseProtein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
582YBR277CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
583YBR278WDPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon)Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
584YBR279WPAF1Component of the Paf1p complex involved in transcription elongationComponent of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
585YBR280CSAF1F-Box protein involved in proteasome-dependent degradation of Aah1pF-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1
586YBR281CDUG2Component of glutamine amidotransferase (GATase II)Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
587YBR282WMRPL27Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
588YBR283CSSH1Subunit of the Ssh1 translocon complexSubunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
589YBR284WYBR284WPutative metallo-dependent hydrolase superfamily proteinPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
590YBR285WYBR285WPutative protein of unknown functionPutative protein of unknown function; YBR285W is not an essential gene
591YBR286WAPE3Vacuolar aminopeptidase YVacuolar aminopeptidase Y; processed to mature form by Prb1p
592YBR287WYBR287WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
593YBR288CAPM3Mu3-like subunit of the clathrin associated protein complex (AP-3)Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
594YBR289WSNF5Subunit of the SWI/SNF chromatin remodeling complexSubunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
595YBR290WBSD2Heavy metal ion homeostasis proteinHeavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
596YBR291CCTP1Mitochondrial inner membrane citrate transporterMitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family
597YBR292CYBR292CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
598YBR293WVBA2Permease of basic amino acids in the vacuolar membranePermease of basic amino acids in the vacuolar membrane
599YBR294WSUL1High affinity sulfate permease of the SulP anion transporter familyHigh affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
600YBR295WPCA1Cadmium transporting P-type ATPaseCadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
601YBR296CPHO89Plasma membrane Na+/Pi cotransporterPlasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
602YBR296C-AYBR296C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
603YBR297WMAL33MAL-activator proteinMAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C
604YBR298CMAL31Maltose permeaseMaltose permease; high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C
605YBR298C-AYBR298C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
606YBR299WMAL32Maltase (alpha-D-glucosidase)Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
607YBR300CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene
608YBR301WPAU24Cell wall mannoproteinCell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
609YBR302CCOS2Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
610YCL001WRER1Protein involved in retention of membrane proteinsProtein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
611YCL001W-AYCL001W-APutative protein of unknown functionPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
612YCL001W-BYCL001W-BPutative protein of unknown functionPutative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
613YCL002CYCL002CPutative protein of unknown functionPutative protein of unknown function; YCL002C is not an essential gene
614YCL004WPGS1Phosphatidylglycerolphosphate synthasePhosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
615YCL005WLDB16Protein involved in lipid droplet (LD) assemblyProtein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
616YCL005W-AVMA9Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplexVacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis
617YCL007CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A
618YCL008CSTP22Component of the ESCRT-I complexComponent of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype;
619YCL009CILV6Regulatory subunit of acetolactate synthaseRegulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
620YCL010CSGF29Component of the HAT/Core module of the SAGA, SLIK, and ADA complexesComponent of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
621YCL011CGBP2Poly(A+) RNA-binding proteinPoly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication
622YCL012CYCL012CProtein of unknown functionPutative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
623YCL014WBUD3Guanine nucleotide exchange factor (GEF) for Cdc42pProtein involved in bud-site selection; required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
624YCL016CDCC1Subunit of a complex with Ctf8p and Ctf18pSubunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance
625YCL017CNFS1Cysteine desulfuraseCysteine desulfurase; involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
626YCL018WLEU2Beta-isopropylmalate dehydrogenase (IMDH)Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of β-ethylmalate into α-ketovalerate
627YCL019WYCL019WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
628YCL020WYCL020WRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
629YCL021W-AYCL021W-APutative protein of unknown functionPutative protein of unknown function
630YCL022CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W
631YCL023CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4
632YCL024WKCC4Protein kinase of the bud neck involved in the septin checkpointProtein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication
633YCL025CAGP1Low-affinity amino acid permease with broad substrate rangeLow-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
634YCL026C-AFRM2Type II nitroreductase, using NADH as reductantType II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
635YCL026C-BHBN1Protein of unknown functionProtein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
636YCL027WFUS1Membrane protein localized to the shmoo tipMembrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
637YCL028WRNQ1[PIN(+)] prion[PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate
638YCL029CBIK1Microtubule-associated proteinMicrotubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170
639YCL030CHIS4Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphataseMultifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
640YCL031CRRP7Essential protein involved in rRNA processing and ribosome biogenesisEssential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
641YCL032WSTE50Adaptor protein for various signaling pathwaysAdaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
642YCL033CMXR2Methionine-R-sulfoxide reductaseMethionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
643YCL034WLSB5Protein involved in membrane-trafficking events at plasma membraneProtein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat
644YCL035CGRX1Glutathione-dependent disulfide oxidoreductaseGlutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
645YCL036WGFD2Protein of unknown functionProtein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication
646YCL037CSRO9Cytoplasmic RNA-binding proteinCytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
647YCL038CATG22Vacuolar integral membrane protein required for efflux of amino acidsVacuolar integral membrane protein required for efflux of amino acids; required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation
648YCL039WGID7Subunit of GID Complex that binds directly to central component Vid30pSubunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions
649YCL040WGLK1GlucokinaseGlucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
650YCL041CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
651YCL042WYCL042WPutative protein of unknown functionPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
652YCL043CPDI1Protein disulfide isomeraseProtein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
653YCL044CMGR1Subunit of the mitochondrial (mt) i-AAA protease supercomplexSubunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
654YCL045CEMC1Member of conserved endoplasmic reticulum membrane complexMember of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
655YCL046WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
656YCL047CPOF1Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT)Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion
657YCL048WSPS22Protein of unknown functionProtein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
658YCL048W-AYCL048W-APutative protein of unknown functionPutative protein of unknown function; YCL048W-A has a paralog, YDR524C-B, that arose from the whole genome duplication
659YCL049CYCL049CProtein of unknown functionProtein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene
660YCL050CAPA1AP4A phosphorylaseAP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication
661YCL051WLRE1Protein involved in control of cell wall structure and stress responseProtein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication
662YCL052CPBN1Component of glycosylphosphatidylinositol-mannosyltransferase IComponent of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X
663YCL054WSPB1AdoMet-dependent methyltransferaseAdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
664YCL055WKAR4Transcription factor required for response to pheromonesTranscription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone
665YCL056CPEX34Protein that regulates peroxisome populationsProtein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
666YCL057C-AMIC10Conserved component of the MICOS complexConserved component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; ortholog of human MINOS1
667YCL057WPRD1Zinc metalloendopeptidaseZinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
668YCL058CFYV5Protein involved in regulation of the mating pathwayProtein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis
669YCL058W-AADF1Transcriptional repressor encoded by the FYV5 antisense strandTranscriptional repressor encoded by the FYV5 antisense strand; negatively regulates transcription of FYV5 by binding to the promoter on the sense strand
670YCL059CKRR1Nucleolar protein required for rRNA synthesis and ribosomal assemblyNucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene
671YCL061CMRC1S-phase checkpoint protein required for DNA replicationS-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle
672YCL063WVAC17Phosphoprotein involved in vacuole inheritancePhosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p
673YCL064CCHA1Catabolic L-serine (L-threonine) deaminaseCatabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
674YCL065WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps HMLALPHA1
675YCL066WHMLALPHA1Silenced copy of ALPHA1 at HMLSilenced copy of ALPHA1 at HML; ALPHA1 encodes a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression
676YCL067CHMLALPHA2Silenced copy of ALPHA2 at HMLSilenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression
677YCL068CYCL068CPutative protein of unknown functionPutative protein of unknown function
678YCL069WVBA3Permease of basic amino acids in the vacuolar membranePermease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication
679YCL073CGEX1Proton:glutathione antiporterProton:glutathione antiporter; localized to the vacuolar and plasma membranes; imports glutathione from the vacuole and exports it through the plasma membrane; has a role in resistance to oxidative stress and modulation of the PKA pathway; GEX1 has a paralog, GEX2, that arose from a segmental duplication
680YCL076WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
681YCR001WYCR001WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene
682YCR002CCDC10Component of the septin ring, required for cytokinesisComponent of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
683YCR003WMRPL32Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
684YCR004CYCP4Protein of unknown functionProtein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
685YCR005CCIT2Citrate synthase, peroxisomal isozyme involved in glyoxylate cycleCitrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
686YCR006CYCR006CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
687YCR007CYCR007CPutative integral membrane proteinPutative integral membrane protein; member of DUP240 gene family; YCR007C is not an essential gene
688YCR008WSAT4Ser/Thr protein kinase involved in salt toleranceSer/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion
689YCR009CRVS161Amphiphysin-like lipid raft proteinAmphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
690YCR010CADY2Acetate transporter required for normal sporulationAcetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
691YCR011CADP1Putative ATP-dependent permease of the ABC transporter familyPutative ATP-dependent permease of the ABC transporter family
692YCR012WPGK13-phosphoglycerate kinase3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
693YCR013CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
694YCR014CPOL4DNA polymerase IVDNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
695YCR015CCTO1Protein required for cold tolerancePutative protein of unknown function; YCR015C is not an essential gene
696YCR016WYCR016WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
697YCR017CCWH43GPI lipid remodelasePutative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
698YCR018CSRD1Protein involved in the processing of pre-rRNA to mature rRNAProtein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation
699YCR018C-APutative protein of unknown function; encoded opposite a Ty1 LTR
700YCR019WMAK32Protein necessary for stability of L-A dsRNA-containing particlesProtein necessary for stability of L-A dsRNA-containing particles
701YCR020CPET18Protein of unknown functionProtein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
702YCR020C-AMAK31Non-catalytic subunit of N-terminal acetyltransferase of the NatC typeNon-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
703YCR020W-BHTL1Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
704YCR021CHSP30Negative regulator of the H(+)-ATPase Pma1pNegative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
705YCR022CYCR022CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene
706YCR023CYCR023CVacuolar membrane protein of unknown functionVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
707YCR024CSLM5Mitochondrial asparaginyl-tRNA synthetaseMitochondrial asparaginyl-tRNA synthetase
708YCR024C-APMP1Regulatory subunit for the plasma membrane H(+)-ATPase Pma1pRegulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
709YCR024C-BYCR024C-BPutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
710YCR025CYCR025CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
711YCR026CNPP1Nucleotide pyrophosphatase/phosphodiesteraseNucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants
712YCR027CRHB1Putative Rheb-related GTPasePutative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
713YCR028CFEN2Plasma membrane H+-pantothenate symporterPlasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress
714YCR028C-ARIM1ssDNA-binding protein essential for mitochondrial genome maintenancessDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication
715YCR030CSYP1Negative regulator of WASP-Arp23 complexNegative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
716YCR031CRPS14AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
717YCR032WBPH1Protein homologous to Chediak-Higashi syndrome and Beige proteinsProtein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
718YCR033WSNT1Subunit of the Set3C deacetylase complexSubunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia
719YCR034WELO2Fatty acid elongase, involved in sphingolipid biosynthesisFatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication
720YCR035CRRP43Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
721YCR036WRBK1Putative ribokinasePutative ribokinase
722YCR037CPHO87Low-affinity inorganic phosphate (Pi) transporterLow-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
723YCR038CBUD5GTP/GDP exchange factor for Rsr1p (Bud1p)GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types
724YCR038W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene BUD5/YCR038C; identified by homology with hemiascomycetous yeast species
725YCR039CMATALPHA2Homeobox-domain proteinHomeobox-domain protein; with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette
726YCR040WMATALPHA1Transcriptional co-activator that regulates mating-type-specific genesTranscriptional co-activator that regulates mating-type-specific genes; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette
727YCR041WYCR041WProtein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
728YCR042CTAF2TFIID subunit (150 kDa)TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation
729YCR043CYCR043CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
730YCR044CPER1Protein of the endoplasmic reticulumProtein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
731YCR045CRRT12Probable subtilisin-family proteaseProbable subtilisin-family protease; role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores
732YCR045W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with YCR045C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
733YCR046CIMG1Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome
734YCR047CBUD23Methyltransferase that methylates residue G1575 of 18S rRNAMethyltransferase; methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
735YCR047W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with YCR047C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
736YCR048WARE1Acyl-CoA:sterol acyltransferaseAcyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
737YCR049CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
738YCR050CYCR050CNon-essential protein of unknown functionNon-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
739YCR051WYCR051WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
740YCR052WRSC6Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication
741YCR053WTHR4Threonine synthaseThreonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
742YCR054CCTR86Essential protein of unknown functionEssential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
743YCR057CPWP2Conserved 90S pre-ribosomal componentConserved 90S pre-ribosomal component; essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
744YCR059CYIH1Negative regulator of eIF2 kinase Gcn2pNegative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
745YCR060WTAH1Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1)Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants
746YCR061WYCR061WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
747YCR063WBUD31Component of the SF3b subcomplex of the U2 snRNPComponent of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
748YCR064CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BUD31
749YCR065WHCM1Forkhead transcription factorForkhead transcription factor; drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role; regulates replicative lifespan; ortholog of C. elegans lifespan regulator PHA-4
750YCR066WRAD18E3 ubiquitin ligaseE3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
751YCR067CSED4Integral ER membrane protein that stimulates Sar1p GTPase activityIntegral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication
752YCR068WATG15PhospholipaseLipase required for intravacuolar lysis of autophagic and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
753YCR069WCPR4Peptidyl-prolyl cis-trans isomerase (cyclophilin)Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication
754YCR071CIMG2Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49
755YCR072CRSA4WD-repeat protein involved in ribosome biogenesisWD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
756YCR073CSSK22MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathwayMAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication
757YCR073W-ASOL2Protein with a possible role in tRNA exportProtein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
758YCR075CERS1Protein involved in cystine transportProtein with similarity to human cystinosin; cystinosin is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains
759YCR075W-AEGO2Subunit of the EGO/GSE complexPutative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
760YCR076CFUB1Proteasome-binding proteinProteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
761YCR077CPAT1Deadenylation-dependent mRNA-decapping factorDeadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation
762YCR079WPTC6Mitochondrial type 2C protein phosphatase (PP2C)Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
763YCR081C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with SRB8/YCR081W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
764YCR081WSRB8Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
765YCR082WAHC2Component of the ADA histone acetyltransferase complexComponent of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
766YCR083WTRX3Mitochondrial thioredoxinMitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
767YCR084CTUP1General repressor of transcriptionGeneral repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
768YCR085WYCR085WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
769YCR086WCSM1Nucleolar protein that mediates homolog segregation during meiosis INucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
770YCR087C-AYCR087C-APutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
771YCR087WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
772YCR088WABP1Actin-binding protein of the cortical actin cytoskeletonActin-binding protein of the cortical actin cytoskeleton; important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; inhibits barbed-end actin filament elongation; phosphorylation within its Proline-Rich Regio, mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences; mammalian homologue of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa)
773YCR089WFIG2Cell wall adhesin, expressed specifically during matingCell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication
774YCR090CYCR090CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
775YCR091WKIN82Putative serine/threonine protein kinasePutative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; KIN82 has a paralog, FPK1, that arose from the whole genome duplication
776YCR092CMSH3Mismatch repair proteinMismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
777YCR093WCDC39Subunit of the CCR4-NOT1 core complexComponent of the CCR4-NOT1 core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
778YCR094WCDC50Endosomal protein that interacts with phospholipid flippase Drs2pEndosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
779YCR095COCA4Cytoplasmic protein required for replication of Brome mosaic virusCytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
780YCR095W-AYCR095W-APutative protein of unknown functionPutative protein of unknown function
781YCR096CHMRA2Silenced copy of a2 at HMRSilenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system
782YCR097WHMRA1Silenced copy of a1 at HMRSilenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells
783YCR097W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by homology to a hemiascomycetous yeast protein
784YCR098CGIT1Plasma membrane permeasePlasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
785YCR099CYCR099CPutative protein of unknown functionPutative protein of unknown function
786YCR100CYCR100CPutative protein of unknown functionPutative protein of unknown function
787YCR101CYCR101CPutative protein of unknown functionPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
788YCR102CYCR102CPutative protein of unknown functionPutative protein of unknown function; involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family
789YCR102W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
790YCR104WPAU3Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
791YCR105WADH7NADPH-dependent medium chain alcohol dehydrogenaseNADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance
792YCR106WRDS1Putative zinc cluster transcription factorPutative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
793YCR107WAAD3Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD3 has a paralog, AAD15, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
794YCR108CYCR108CPutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
795YDL001WRMD1Cytoplasmic protein required for sporulationCytoplasmic protein required for sporulation
796YDL002CNHP10Non-essential INO80 chromatin remodeling complex subunitNon-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins
797YDL003WMCD1Essential alpha-kleisin subunit of the cohesin complexEssential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
798YDL004WATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthaseDelta subunit of the central stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
799YDL005CMED2Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia
800YDL006WPTC1Type 2C protein phosphatase (PP2C)Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
801YDL007C-AYDL007C-APutative protein of unknown functionPutative protein of unknown function
802YDL007WRPT2ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome
803YDL008WAPC11Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C)Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
804YDL009CYDL009CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
805YDL010WGRX6Cis-golgi localized monothiol glutaredoxin, binds Fe-S clusterCis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
806YDL011CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W
807YDL012CYDL012CTail-anchored plasma membrane protein with a conserved CYSTM moduleTail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
808YDL013WSLX5Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complexSubunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase complex; stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers; the SUMO-targeted ubiquitin ligase complex is also known as the STUbL complex
809YDL014WNOP1Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolusHistone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin
810YDL015CTSC13Enoyl reductaseEnoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
811YDL016CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
812YDL017WCDC7Ser/Thr kinase and catalytic subunit of DDK (Dbf4-dependent kinase)DDK (Dbf4-dependent kinase) catalytic subunit; required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
813YDL018CERP3Protein with similarity to Emp24p and Erv25pProtein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
814YDL019COSH2Member of an oxysterol-binding protein family with seven membersMember of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH2 has a paralog, SWH1, that arose from the whole genome duplication
815YDL020CRPN4Transcription factor that stimulates expression of proteasome genesTranscription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
816YDL021WGPM2Nonfunctional homolog of Gpm1p phosphoglycerate mutaseHomolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM2 has a paralog, GPM3, that arose from the whole genome duplication
817YDL022C-AYDL022C-AProtein of unknown functionProtein of unknown function; partially overlaps the verified gene DIA3; identified by fungal homology and RT-PCR; mRNA identified as translated by ribosome profiling data
818YDL022WGPD1NAD-dependent glycerol-3-phosphate dehydrogenaseNAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity; GPD1 has a paralog, GPD2, that arose from the whole genome duplication
819YDL023CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance
820YDL024CDIA3Protein of unknown functionProtein of unknown function; involved in invasive and pseudohyphal growth
821YDL025CRTK1Putative protein kinase, potentially phosphorylated by Cdc28pPutative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
822YDL025W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the ORF YDL025C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
823YDL026WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
824YDL027CMRX9Protein that associates with mitochondrial ribosomePutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
825YDL028CMPS1Dual-specificity kinaseDual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
826YDL029WARP2Essential component of the Arp2/3 complexEssential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants
827YDL030WPRP9Subunit of the SF3a splicing factor complexSubunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
828YDL031WDBP10Putative ATP-dependent RNA helicase of the DEAD-box protein familyPutative ATP-dependent RNA helicase of the DEAD-box protein family; constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
829YDL032WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene
830YDL033CSLM3tRNA-specific 2-thiouridylasetRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
831YDL034WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
832YDL035CGPR1Plasma membrane G protein coupled receptor (GPCR)Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
833YDL036CPUS9Mitochondrial tRNA:pseudouridine synthaseMitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
834YDL037CBSC1Protein of unconfirmed functionProtein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
835YDL039CPRM7Pheromone-regulated proteinPheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
836YDL040CNAT1Subunit of protein N-terminal acetyltransferase NatASubunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
837YDL041WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
838YDL042CSIR2Conserved NAD+ dependent histone deacetylase of the Sirtuin familyConserved NAD+ dependent histone deacetylase of the Sirtuin family; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication; functions as a regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy; SIR2 has a paralog, HST1, that arose from the whole genome duplication
839YDL043CPRP11Subunit of the SF3a splicing factor complexSubunit of the SF3a splicing factor complex; required for spliceosome assembly
840YDL044CMTF2Mitochondrial protein that interacts with mitochondrial RNA polymeraseMitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
841YDL045CFAD1Flavin adenine dinucleotide (FAD) synthetaseFlavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin
842YDL045W-AMRP10Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria
843YDL046WNPC2Sterol transport protein and functional homolog of human NPC2/He1Functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes
844YDL047WSIT4Ceramide-activated, type 2A-related serine-threonine phosphataseType 2A-related serine-threonine phosphatase; functions in the G1/S transition of the mitotic cycle; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6
845YDL048CSTP4Protein containing a Kruppel-type zinc-finger domainProtein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication
846YDL049CKNH1Protein with similarity to Kre9pProtein with similarity to Kre9p; Kre9p is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
847YDL050CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
848YDL051WLHP1RNA binding protein required for maturation of tRNA and U6 snRNARNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
849YDL052CSLC11-acyl-sn-glycerol-3-phosphate acyltransferase1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
850YDL053CPBP4Pbp1p binding proteinPbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
851YDL054CMCH1Protein with similarity to mammalian monocarboxylate permeasesProtein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
852YDL055CPSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase)GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase); synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
853YDL056WMBP1Transcription factorTranscription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
854YDL057WYDL057WPutative protein of unknown functionPutative protein of unknown function; YDL057W is not an essential gene
855YDL058WUSO1Essential protein involved in vesicle-mediated ER to Golgi transportEssential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
856YDL059CRAD59Protein involved DNA double-strand break repairProtein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
857YDL060WTSR1Protein required for processing of 20S pre-rRNA in the cytoplasmProtein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
858YDL061CRPS29BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
859YDL062WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential
860YDL063CSYO1Transport adaptor or symportinTransport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
861YDL064WUBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathwaySUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
862YDL065CPEX19Chaperone and import receptor for newly-synthesized class I PMPsChaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
863YDL066WIDP1Mitochondrial NADP-specific isocitrate dehydrogenaseMitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
864YDL067CCOX9Subunit VIIa of cytochrome c oxidase (Complex IV)Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
865YDL068WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
866YDL069CCBS1Mitochondrial translational activator of the COB mRNAMitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
867YDL070WBDF2Protein involved in transcription initiationProtein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
868YDL071CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W
869YDL072CYET3Protein of unknown functionProtein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
870YDL073WAHK1Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathwayPutative protein of unknown function; YDL073W is not an essential gene
871YDL074CBRE1E3 ubiquitin ligaseE3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing
872YDL075WRPL31ARibosomal 60S subunit protein L31ARibosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
873YDL076CRXT3Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
874YDL077CVAM6Guanine nucleotide exchange factor for the GTPase Gtr1pSubunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p, facilitating tethering and promoting several membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking
875YDL078CMDH3Peroxisomal malate dehydrogenasePeroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle
876YDL079CMRK1Glycogen synthase kinase 3 (GSK-3) homologGlycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication
877YDL080CTHI3Regulatory protein that binds Pdc2p and Thi2p transcription factorsRegulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected
878YDL081CRPP1ARibosomal stalk protein P1 alphaRibosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
879YDL082WRPL13ARibosomal 60S subunit protein L13ARibosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
880YDL083CRPS16BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
881YDL084WSUB2Component of the TREX complex required for nuclear mRNA exportComponent of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
882YDL085C-AYDL085C-APutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
883YDL085WNDE2Mitochondrial external NADH dehydrogenaseMitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication
884YDL086C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps uncharacterized ORF YDL086W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
885YDL086WYDL086WPutative carboxymethylenebutenolidasePutative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
886YDL087CLUC7Essential protein associated with the U1 snRNP complexEssential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia
887YDL088CASM4FG-nucleoporin component of central core of nuclear pore complex (NPC)FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
888YDL089WNUR1Protein involved in regulation of mitotic exitProtein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate
889YDL090CRAM1Beta subunit of the CAAX farnesyltransferase (FTase)Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
890YDL091CUBX3Clathrin-coated vesicle component, regulator of endocytosisSubunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10
891YDL092WSRP14Signal recognition particle (SRP) subunitSignal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
892YDL093WPMT5Protein O-mannosyltransferaseProtein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals
893YDL094CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential
894YDL095WPMT1Protein O-mannosyltransferase of the ER membraneProtein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
895YDL096CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
896YDL097CRPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lidEssential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
897YDL098CSNU23Component of the U4/U6.U5 snRNP complexComponent of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome
898YDL099WBUG1Cis-golgi localized protein involved in ER to Golgi transportCis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
899YDL100CGET3Guanine nucleotide exchange factor for Gpa1pGuanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of the GET complex, which is involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into the ER membrane under non-stress conditions; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
900YDL101CDUN1Cell-cycle checkpoint S/T protein kinaseCell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair
901YDL102WPOL3Catalytic subunit of DNA polymerase deltaCatalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
902YDL103CQRI1UDP-N-acetylglucosamine pyrophosphorylaseUDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
903YDL104CQRI7Protein involved in threonylcarbamoyl adenosine biosynthesisProtein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex
904YDL105WNSE4Component of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
905YDL106CPHO2Homeobox transcription factorHomeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
906YDL107WMSS2Peripherally bound inner membrane protein of the mitochondrial matrixPeripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
907YDL108WKIN28Serine/threonine protein kinase, subunit of transcription factor TFIIHSerine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia
908YDL109CYDL109CPutative lipasePutative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication
909YDL110CTMA17ATPase dedicated chaperone that adapts proteasome assembly to stressATPase dedicated chaperone that adapts proteasome assembly to stress; Tma17p is induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
910YDL111CRRP42Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
911YDL112WTRM32'-O-ribose methyltransferase2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
912YDL113CATG20Sorting nexin family memberSorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
913YDL114WYDL114WPutative short-chain dehydrogenase/reductasePutative short-chain dehydrogenase/reductase; YDL114W is not an essential gene
914YDL114W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified based on homology to hemiascomycetous yeasts
915YDL115CIWR1RNA polymerase II transport factor, conserved from yeast to humansRNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
916YDL116WNUP84Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
917YDL117WCYK3SH3-domain protein located in the bud neck and cytokinetic actin ringSH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
918YDL118WDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
919YDL119CHEM25Mitochondrial glycine transporterPutative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
920YDL120WYFH1Mitochondrial matrix iron chaperoneMitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
921YDL121CYDL121CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein
922YDL122WUBP1Ubiquitin-specific proteaseUbiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
923YDL123WSNA4Protein of unknown functionProtein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated
924YDL124WYDL124WNADPH-dependent alpha-keto amide reductaseNADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
925YDL125CHNT1Adenosine 5'-monophosphoramidaseAdenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress
926YDL126CCDC48AAA ATPaseAAA ATPase; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing, in macroautophagy, PMN, RAD, ribophagy, homotypic ER membrane fusion, disassembly of Met30p from SCF complex; functional ortholog of human p97/VCP
927YDL127WPCL2Cyclin, interacts with cyclin-dependent kinase Pho85pCyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication
928YDL128WVCX1Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activityVacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
929YDL129WYDL129WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
930YDL130WRPP1BRibosomal protein P1 betaRibosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component
931YDL130W-ASTF1Protein involved in regulation of the mitochondrial F1F0-ATP synthaseProtein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication
932YDL131WLYS21Homocitrate synthase isozymeHomocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
933YDL132WCDC53CullinCullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
934YDL133C-ARPL41BRibosomal 60S subunit protein L41BRibosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
935YDL133WSRF1Regulator of phospholipase D (Spo14p)Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
936YDL134CPPH21Catalytic subunit of protein phosphatase 2A, PP2ACatalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
937YDL135CRDI1Rho GDP dissociation inhibitorRho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
938YDL136WRPL35BRibosomal 60S subunit protein L35BRibosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
939YDL137WARF2ADP-ribosylation factorADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication
940YDL138WRGT2Plasma membrane high glucose sensor that regulates glucose transportPlasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
941YDL139CSCM3Nonhistone component of centromeric chromatinNonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP
942YDL140CRPO21RNA polymerase II largest subunit B220RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
943YDL141WBPL1Biotin:apoprotein ligaseBiotin:apoprotein ligase; covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
944YDL142CCRD1Cardiolipin synthaseCardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
945YDL143WCCT4Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
946YDL144CYDL144CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
947YDL145CCOP1Alpha subunit of COPI vesicle coatomer complexAlpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway
948YDL146WLDB17Protein involved in the regulation of endocytosisProtein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
949YDL147WRPN5Subunit of the CSN and 26S proteasome lid complexesSubunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN
950YDL148CNOP14Nucleolar proteinNucleolar protein; forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
951YDL149WATG9Transmembrane protein involved in forming Cvt and autophagic vesiclesTransmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS
952YDL150WRPC53RNA polymerase III subunit C53RNA polymerase III subunit C53
953YDL151CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay
954YDL152WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
955YDL153CSAS10Subunit of U3-containing Small Subunit (SSU) processome complexSubunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene
956YDL154WMSH5Protein of the MutS familyProtein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
957YDL155WCLB3B-type cyclin involved in cell cycle progressionB-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
958YDL156WCMR1Nuclear protein with a role in protein quality controlDNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS
959YDL157CYDL157CPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
960YDL158CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
961YDL159C-BPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
962YDL159WSTE7Signal transducing MAP kinase kinaseSignal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
963YDL159W-AYDL159W-APutative protein of unknown functionPutative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
964YDL160CDHH1Cytoplasmic DEAD-box helicase, stimulates mRNA decappingCytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, role in translational repression, mRNA decay, and processing body dynamics; may have a role in mRNA export; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
965YDL160C-AMHF2Component of the heterotetrameric MHF histone-fold complexComponent of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2
966YDL161WENT1Epsin-like protein involved in endocytosis and actin patch assemblyEpsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
967YDL162CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
968YDL163WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
969YDL164CCDC9DNA ligase I found in nucleus and mitochondriaDNA ligase found in the nucleus and mitochondria; an essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination,
970YDL165WCDC36Component of the CCR4-NOT core complex, involved in mRNA decappingComponent of the CCR4-NOT core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor
971YDL166CFAP7Essential NTPase required for small ribosome subunit synthesisEssential NTPase required for small ribosome subunit synthesis; mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D; depletion leads to accumulation of pre-40S ribosomes in 80S-like ribosomes
972YDL167CNRP1Putative RNA binding protein of unknown functionPutative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
973YDL168WSFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenaseBifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
974YDL169CUGX2Protein of unknown functionProtein of unknown function; transcript accumulates in response to any combination of stress conditions
975YDL170WUGA3Transcriptional activator for GABA-dependent induction of GABA genesTranscriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4
976YDL171CGLT1NAD(+)-dependent glutamate synthase (GOGAT)NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions
977YDL172CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
978YDL173WPAR32Low complexity proteinPutative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene
979YDL174CDLD1Major mitochondrial D-lactate dehydrogenaseD-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
980YDL175CAIR2RNA-binding subunit of the TRAMP nuclear RNA surveillance complexRNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
981YDL176WYDL176WProtein of unknown functionProtein of unknown function; predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
982YDL177CYDL177CPutative protein of unknown functionPutative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
983YDL178WDLD2D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenaseD-lactate dehydrogenase; located in the mitochondrial matrix
984YDL179WPCL9CyclinCyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication
985YDL180WYDL180WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
986YDL181WINH1Protein that inhibits ATP hydrolysis by the F1F0-ATP synthaseProtein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication
987YDL182WLYS20Homocitrate synthase isozyme and functions in DNA repairHomocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
988YDL183CYDL183CProtein that may form an active mitochondrial KHE systemProtein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system
989YDL184CRPL41ARibosomal 60S subunit protein L41ARibosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
990YDL185C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDL185W; identified by homology with hemiascomycetous yeast species
991YDL185WVMA1Subunit A of the V1 peripheral membrane domain of V-ATPaseSubunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
992YDL186WYDL186WPutative protein of unknown functionPutative protein of unknown function; YDL186W is not an essential gene
993YDL187CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
994YDL188CPPH22Catalytic subunit of protein phosphatase 2A, PP2ACatalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
995YDL189WRBS1Protein involved in assembly of the RNA polymerase III (Pol III) compProtein of unknown function; identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
996YDL190CUFD2Ubiquitin chain assembly factor (E4)Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
997YDL191WRPL35ARibosomal 60S subunit protein L35ARibosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication
998YDL192WARF1ADP-ribosylation factorADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
999YDL193WNUS1Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1Putative prenyltransferase; required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
1000YDL194WSNF3Plasma membrane low glucose sensor, regulates glucose transportPlasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication
1001YDL195WSEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coatComponent of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
1002YDL196WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
1003YDL197CASF2Anti-silencing proteinAnti-silencing protein; causes derepression of silent loci when overexpressed
1004YDL198CGGC1Mitochondrial GTP/GDP transporterMitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
1005YDL199CYDL199CPutative transporterPutative transporter; member of the sugar porter family
1006YDL200CMGT1DNA repair methyltransferase (6-O-methylguanine-DNA methylase)DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage
1007YDL201WTRM8Catalytic subunit of a tRNA methyltransferase complexNoncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
1008YDL202WMRPL11Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
1009YDL203CACK1Protein that functions in the cell wall integrity pathwayProtein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
1010YDL204WRTN2Reticulon proteinReticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication
1011YDL205CHEM3Porphobilinogen deaminasePorphobilinogen deaminase; catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
1012YDL206WYDL206WPutative protein of unknown functionPutative protein of unknown function; YDL206W is not an essential protein
1013YDL207WGLE1Cytoplasmic nucleoporin required for polyadenylated mRNA exportCytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination
1014YDL208WNHP2Protein related to mammalian high mobility group (HMG) proteinsProtein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
1015YDL209CCWC2Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
1016YDL210WUGA4GABA (gamma-aminobutyrate) permeaseGABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
1017YDL211CYDL211CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication
1018YDL212WSHR3Endoplasmic reticulum packaging chaperoneEndoplasmic reticulum packaging chaperone; required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
1019YDL213CNOP6rRNA-binding protein required for 40S ribosomal subunit biogenesisrRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
1020YDL214CPRR2Serine/threonine protein kinaseSerine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication
1021YDL215CGDH2NAD(+)-dependent glutamate dehydrogenaseNAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis
1022YDL216CRRI1Catalytic subunit of the COP9 signalosome (CSN) complexCatalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling
1023YDL217CTIM22Essential core component of the mitochondrial TIM22 complexEssential core component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
1024YDL218WYDL218WPutative protein of unknown functionPutative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
1025YDL219WDTD1D-Tyr-tRNA(Tyr) deacylaseD-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
1026YDL220CCDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tailsSingle stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle
1027YDL221WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
1028YDL222CFMP45Integral membrane protein localized to mitochondriaIntegral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication
1029YDL223CHBT1Shmoo tip protein, substrate of Hub1p ubiquitin-like proteinShmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication
1030YDL224CWHI4Putative RNA binding proteinPutative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
1031YDL225WSHS1Component of the septin ring that is required for cytokinesisComponent of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
1032YDL226CGCS1ADP-ribosylation factor GTPase activating protein (ARF GAP)ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication
1033YDL227CHOSite-specific endonucleaseSite-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p
1034YDL228CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene SSB1
1035YDL229WSSB1Cytoplasmic ATPase that is a ribosome-associated molecular chaperoneCytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
1036YDL230WPTP1Phosphotyrosine-specific protein phosphatasePhosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
1037YDL231CBRE4Zinc finger protein containing five transmembrane domainsZinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
1038YDL232WOST4Subunit of the oligosaccharyltransferase complex of the ER lumenSubunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
1039YDL233WMFG1Regulator of filamentous growthRegulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
1040YDL234CGYP7GTPase-activating protein for yeast Rab family membersGTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking
1041YDL235CYPD1Osmotic stress-responsive phosphorelay intermediate sensor proteinOsmotic stress-responsive phosphorelay intermediate sensor protein; phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
1042YDL236WPHO13Conserved phosphatase acting as a metabolite repair enzymeAlkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity
1043YDL237WAIM6Protein of unknown functionPutative protein of unknown function; required for respiratory growth; YDL237W is not an essential gene
1044YDL238CGUD1Guanine deaminaseGuanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
1045YDL239CADY3Protein required for spore wall formationProtein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication
1046YDL240C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species
1047YDL240WLRG1GTPase-activating protein (GAP)GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
1048YDL241WYDL241WPutative protein of unknown functionPutative protein of unknown function; YDL241W is not an essential gene
1049YDL242WYDL242WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1050YDL243CAAD4Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
1051YDL244WTHI13Protein involved in synthesis of the thiamine precursor HMPProtein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
1052YDL245CHXT15Putative transmembrane polyol transporterProtein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose
1053YDL246CSOR2Sorbitol dehydrogenaseProtein of unknown function; protein sequence is 99% identical to the Sor1p sorbitol dehydrogenase; computational analysis of large-scale protein-protein interaction data also suggests a role in fructose or mannose metabolism
1054YDL247WMPH2Alpha-glucoside permeaseAlpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication
1055YDL247W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species
1056YDL248WCOS7Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
1057YDR001CNTH1Neutral trehalase, degrades trehaloseNeutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
1058YDR002WYRB1Ran GTPase binding proteinRan GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
1059YDR003WRCR2Vacuolar proteinVacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication
1060YDR003W-AYDR003W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
1061YDR004WRAD57Protein that stimulates strand exchangeProtein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
1062YDR005CMAF1Highly conserved negative regulator of RNA polymerase IIIHighly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
1063YDR006CSOK1Protein of unknown functionProtein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
1064YDR007WTRP1Phosphoribosylanthranilate isomerasePhosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
1065YDR008CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps TRP1/YDR007W on opposite strand
1066YDR009WGAL3Transcriptional regulatorTranscriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication
1067YDR010CYDR010CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1068YDR011WSNQ2Plasma membrane ATP-binding cassette (ABC) transporterPlasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
1069YDR012WRPL4BRibosomal 60S subunit protein L4BRibosomal 60S subunit protein L4B; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4B has a paralog, RPL4A, that arose from the whole genome duplication
1070YDR013WPSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
1071YDR014WRAD61Subunit of a complex that inhibits sister chromatid cohesionSubunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin
1072YDR014W-AHED1Meiosis-specific proteinMeiosis-specific protein; down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; prevents the recruitment of Rad54p to site-specific DNA double-strand breaks in vivo; early meiotic gene, transcribed specifically during meiotic prophase
1073YDR015CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A
1074YDR016CDAD1Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
1075YDR017CKCS1Inositol hexakisphosphate and inositol heptakisphosphate kinaseInositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7
1076YDR018CYDR018CProbable membrane protein with three predicted transmembrane domainsProbable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication
1077YDR019CGCV1T subunit of the mitochondrial glycine decarboxylase complexT subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
1078YDR020CDAS2Putative protein of unknown functionPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
1079YDR021WFAL1Nucleolar protein required for maturation of 18S rRNANucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
1080YDR022CATG31Autophagy-specific protein required for autophagosome formationAutophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion
1081YDR023WSES1Cytosolic seryl-tRNA synthetaseCytosolic seryl-tRNA synthetase; class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
1082YDR024WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
1083YDR025WRPS11AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication
1084YDR026CNSI1RNA polymerase I termination factorRNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
1085YDR027CVPS54Component of the GARP (Golgi-associated retrograde protein) complexComponent of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
1086YDR028CREG1Regulatory subunit of type 1 protein phosphatase Glc7pRegulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication
1087YDR029WYDR029WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1088YDR030CRAD28Protein involved in DNA repairProtein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair
1089YDR031WMIX14Mitochondrial intermembrane space protein of unknown functionMitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
1090YDR032CPST2Protein with similarity to a family of flavodoxin-like proteinsProtein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
1091YDR033WMRH1Protein that localizes primarily to the plasma membraneProtein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication
1092YDR034CLYS14Transcriptional activator involved in regulating lysine biosynthesisTranscriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
1093YDR034C-AYDR034C-APutative protein of unknown functionPutative protein of unknown function; contained within the solo Ty1 LTR element YDRWdelta7
1094YDR034C-CYDR034C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1095YDR034C-DYDR034C-DRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1096YDR034W-BYDR034W-BPredicted tail-anchored plasma membrane proteinPredicted tail-anchored plasma membrane protein; contains conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; YDR034W-B has a paralog, YBR056W-A, that arose from the whole genome duplication
1097YDR035WARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
1098YDR036CEHD33-hydroxyisobutyryl-CoA hydrolase3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
1099YDR037WKRS1Lysyl-tRNA synthetaseLysyl-tRNA synthetase
1100YDR038CENA5Protein with similarity to P-type ATPase sodium pumpsProtein with similarity to P-type ATPase sodium pumps; member of the Na+ efflux ATPase family
1101YDR039CENA2P-type ATPase sodium pumpP-type ATPase sodium pump; involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux
1102YDR040CENA1P-type ATPase sodium pumpP-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance
1103YDR041WRSM10Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
1104YDR042CYDR042CPutative protein of unknown functionPutative protein of unknown function; expression is increased in ssu72-ts69 mutant
1105YDR043CNRG1Transcriptional repressorTranscriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose
1106YDR044WHEM13Coproporphyrinogen III oxidaseCoproporphyrinogen III oxidase; an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
1107YDR045CRPC11RNA polymerase III subunit C11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
1108YDR046CBAP3Amino acid permeaseAmino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
1109YDR047WHEM12Uroporphyrinogen decarboxylaseUroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
1110YDR048CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF VMS1/YDR049W
1111YDR049WVMS1Component of a Cdc48p-complex involved in protein quality controlComponent of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
1112YDR050CTPI1Triose phosphate isomerase, abundant glycolytic enzymeTriose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
1113YDR051CDET1Acid phosphataseAcid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
1114YDR052CDBF4Regulatory subunit of Cdc7p-Dbf4p kinase complexRegulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
1115YDR053WPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
1116YDR054CCDC34Ubiquitin-conjugating enzyme (E2)Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
1117YDR055WPST1Cell wall protein that contains a putative GPI-attachment siteCell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication
1118YDR056CEMC10Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
1119YDR057WYOS9ER quality-control lectinER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
1120YDR058CTGL2Triacylglycerol lipase that is localized to the mitochondriaTriacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
1121YDR059CUBC5Ubiquitin-conjugating enzymeUbiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
1122YDR060WMAK21Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
1123YDR061WYDR061WProtein with similarity to ABC transporter family membersProtein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
1124YDR062WLCB2Component of serine palmitoyltransferaseComponent of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
1125YDR063WAIM7Protein that interacts with Arp2/3 complexProtein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
1126YDR064WRPS13Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
1127YDR065WRRG1Protein of unknown functionProtein of unknown function; required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
1128YDR066CRTR2Protein of unknown functionProtein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
1129YDR067COCA6Cytoplasmic protein required for replication of Brome mosaic virusCytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
1130YDR068WDOS2Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1131YDR069CDOA4Ubiquitin hydrolaseUbiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
1132YDR070CFMP16Protein of unknown functionProtein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
1133YDR071CPAA1Polyamine acetyltransferasePolyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
1134YDR072CIPT1InositolphosphotransferaseInositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
1135YDR073WSNF11Subunit of the SWI/SNF chromatin remodeling complexSubunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
1136YDR074WTPS2Phosphatase subunit of the trehalose-6-P synthase/phosphatase complexPhosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
1137YDR075WPPH3Catalytic subunit of protein phosphatase PP4 complexCatalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
1138YDR076WRAD55Protein that stimulates strand exchangeProtein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
1139YDR077WSED1Major stress-induced structural GPI-cell wall glycoproteinMajor stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication
1140YDR078CSHU2Component of Shu complex (aka PCSS complex)Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments
1141YDR079C-ATFB5Component of RNA polymerase II general transcription factor TFIIHComponent of RNA polymerase II general transcription factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; relocalizes to the cytosol in response to hypoxia; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
1142YDR079WPET100Chaperone that facilitates the assembly of cytochrome c oxidaseChaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
1143YDR080WVPS41Subunit of the HOPS endocytic tethering complexSubunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites
1144YDR081CPDC2Transcription factor for thiamine-regulated genesTranscription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
1145YDR082WSTN1Telomere end-binding and capping proteinTelomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1
1146YDR083WRRP8Nucleolar S-adenosylmethionine-dependent rRNA methyltransferaseNucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
1147YDR084CTVP23Integral membrane proteinIntegral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1148YDR085CAFR1Protein required for pheromone-induced projection (shmoo) formationProtein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
1149YDR086CSSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p)Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p); this complex forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
1150YDR087CRRP1Essential evolutionarily conserved nucleolar proteinEssential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles
1151YDR088CSLU7RNA splicing factorRNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
1152YDR089WVTC5Novel subunit of the vacuolar transporter chaperone complexProtein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
1153YDR090CYDR090CPutative protein of unknown functionPutative protein of unknown function
1154YDR091CRLI1Essential Fe-S proteinEssential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress
1155YDR092WUBC13E2 ubiquitin-conjugating enzymeE2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
1156YDR093WDNF2Aminophospholipid translocase (flippase)Aminophospholipid translocase (flippase); localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
1157YDR094WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2/YDR093W
1158YDR095CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1159YDR096WGIS1Histone demethylase and transcription factorHistone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
1160YDR097CMSH6Protein required for mismatch repair in mitosis and meiosisProtein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p
1161YDR098CGRX3Glutathione-dependent oxidoreductaseGlutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication
1162YDR098C-AYDR098C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1163YDR098C-BYDR098C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1164YDR099WBMH214-3-3 protein, minor isoform14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; BMH2 has a paralog, BMH1, that arose from the whole genome duplication
1165YDR100WTVP15Integral membrane proteinIntegral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p
1166YDR101CARX1Nuclear export factor for the ribosomal pre-60S subunitNuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex
1167YDR102CYDR102CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; homozygous diploid deletion strain exhibits high budding index
1168YDR103WSTE5Pheromone-responsive MAPK scaffold proteinPheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
1169YDR104CSPO71Meiosis-specific protein required for prospore membrane morphogenesisMeiosis-specific protein required for spore wall formation; localizes to prospore membrane (PSM) and is required for PSM closure during sporulation; mediates PSM size; interacts with Spo1p and Vps13p and recruits Vps13p to the PSM during sporulation; mutants exhibit reduction in PSM PtdIns-phosphate pools; dispensable for both nuclear divisions during meiosis; contains two PH domains
1170YDR105CTMS1Vacuolar membrane protein of unknown functionVacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
1171YDR106WARP10Component of the dynactin complexComponent of the dynactin complex; localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex
1172YDR107CTMN2Protein with a role in cellular adhesion and filamentous growthProtein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication
1173YDR108WTRS85Component of transport protein particle (TRAPP) complex IIIComponent of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
1174YDR109CYDR109CD-ribulokinasePutative kinase
1175YDR110WFOB1Nucleolar protein that binds the rDNA replication fork barrier siteNucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases
1176YDR111CALT2Catalytically inactive alanine transaminaseCatalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication
1177YDR112WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ALT2/YDR111C; null mutant displays increased levels of spontaneous Rad52p foci
1178YDR113CPDS1SecurinSecurin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
1179YDR114CYDR114CPutative protein of unknown functionPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
1180YDR115WMRX14Putative mitochondrial ribosomal protein of the large subunitPutative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
1181YDR116CMRPL1Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
1182YDR117CTMA64Protein of unknown function that associates with ribosomesProtein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
1183YDR118WAPC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
1184YDR118W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified using a combination of expression profiling and mass spectrometry
1185YDR119WVBA4Protein of unknown functionProtein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
1186YDR119W-ACOX26Stabilizes or regulates formation of respiratory chain supercomplexesPutative protein of unknown function; copurifies with respiratory chain supercomplexes composed of Complex III (ubiquinol-cytochrome c reductase) and Complex IV (cytochrome c oxidase)
1187YDR120CTRM1tRNA methyltransferasetRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB
1188YDR121WDPB4Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complexSubunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
1189YDR122WKIN1Serine/threonine protein kinase involved in regulation of exocytosisSerine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication
1190YDR123CINO2Transcription factorTranscription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift
1191YDR124WYDR124WPutative protein of unknown functionPutative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor
1192YDR125CECM18Protein of unknown functionProtein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
1193YDR126WSWF1Palmitoyltransferase that acts on transmembrane proteinsPalmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
1194YDR127WARO1Pentafunctional arom proteinPentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
1195YDR128WMTC5Subunit of SEACAT, a subcomplex of the SEA complexSubunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
1196YDR129CSAC6Fimbrin, actin-bundling proteinFimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
1197YDR130CFIN1Spindle pole body-related intermediate filament proteinSpindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
1198YDR131CYDR131CF-box protein subunit of SCF ubiquitin ligase complexF-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
1199YDR132CYDR132CProtein of unknown functionProtein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication
1200YDR133CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
1201YDR135CYCF1Vacuolar glutathione S-conjugate transporterVacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
1202YDR136CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
1203YDR137WRGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p)Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p
1204YDR138WHPR1Subunit of THO/TREX complexesSubunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
1205YDR139CRUB1Ubiquitin-like protein with similarity to mammalian NEDD8Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
1206YDR140WMTQ2S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family
1207YDR141CDOP1Golgi-localized, leucine-zipper domain containing proteinGolgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
1208YDR142CPEX7Peroxisomal signal receptor for peroxisomal matrix proteinsPeroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
1209YDR143CSAN1Ubiquitin-protein ligaseUbiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins
1210YDR144CMKC7GPI-anchored aspartyl proteaseGPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication
1211YDR145WTAF12Subunit (61/68 kDa) of TFIID and SAGA complexesSubunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
1212YDR146CSWI5Transcription factor that recruits Mediator and Swi/Snf complexesTranscription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
1213YDR147WEKI1Ethanolamine kinaseEthanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
1214YDR148CKGD2Dihydrolipoyl transsuccinylaseDihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
1215YDR149CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1/YDR150W; null mutation blocks anaerobic growth
1216YDR150WNUM1Protein required for nuclear migrationProtein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and motochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
1217YDR151CCTH1Member of the CCCH zinc finger familyMember of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication
1218YDR152WGIR2Highly-acidic RWD domain-containing cytoplasmic proteinHighly-acidic RWD domain-containing protein of unknown function; cytoplasmic; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein
1219YDR153CENT5Protein containing an N-terminal epsin-like domainProtein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
1220YDR154CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein
1221YDR155CCPR1Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin)Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress
1222YDR156WRPA14RNA polymerase I subunit A14RNA polymerase I subunit A14
1223YDR157WYDR157WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1224YDR158WHOM2Aspartic beta semi-aldehyde dehydrogenaseAspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
1225YDR159WSAC3mRNA export factormRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
1226YDR160WSSY1Component of the SPS plasma membrane amino acid sensor systemComponent of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
1227YDR161WACL4Specific assembly chaperone for ribosomal protein Rpl4pPutative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
1228YDR162CNBP2Protein involved in the HOG (high osmolarity glycerol) pathwayProtein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
1229YDR163WCWC15Non-essential protein involved in pre-mRNA splicingNon-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
1230YDR164CSEC1Sm-like protein involved in docking and fusion of exocytic vesiclesSm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p
1231YDR165WTRM82Noncatalytic subunit of a tRNA methyltransferase complexCatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia
1232YDR166CSEC5Essential 107kDa subunit of the exocyst complexEssential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
1233YDR167WTAF10Subunit (145 kDa) of TFIID and SAGA complexesSubunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
1234YDR168WCDC37Essential Hsp90p co-chaperoneEssential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
1235YDR169CSTB3Ribosomal RNA processing element (RRPE)-binding proteinRibosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
1236YDR169C-AYDR169C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
1237YDR170CSEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factorsGuanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
1238YDR170W-AYDR170W-ARetrotransposon TYA Gag geneRetrotransposon TYA Gag gene; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag; YDR170W-A is part of a mutant retrotransposon; distribution in the cytoplasm becomes irregular rather than punctate upon DNA replication stress
1239YDR171WHSP42Small heat shock protein (sHSP) with chaperone activitySmall heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
1240YDR172WSUP35Translation termination factor eRF3Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
1241YDR173CARG82Inositol polyphosphate multikinase (IPMK)Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
1242YDR174WHMO1Chromatin associated high mobility group (HMG) family memberChromatin associated high mobility group (HMG) family member; involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia
1243YDR175CRSM24Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
1244YDR176WNGG1Subunit of chromatin modifying histone acetyltransferase complexesSubunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes
1245YDR177WUBC1Ubiquitin-conjugating enzymeUbiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)
1246YDR178WSDH4Membrane anchor subunit of succinate dehydrogenase (SDH)Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma
1247YDR179CCSN9Subunit of the Cop9 signalosomeSubunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
1248YDR179W-ANVJ3Protein with a potential role in tethering ER and vacuolesPutative protein of unknown function
1249YDR180WSCC2Subunit of cohesin loading factor (Scc2p-Scc4p)Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin; relocalizes to the cytosol in response to hypoxia
1250YDR181CSAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p)Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
1251YDR182WCDC1Putative mannose-ethanolamine phosphate phosphodiesterasePutative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
1252YDR182W-AYDR182W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
1253YDR183C-ADubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR
1254YDR183WPLP1Protein that interacts with CCT (chaperonin containing TCP-1) complexProtein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
1255YDR184CATC1Nuclear proteinNuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
1256YDR185CUPS3Mitochondrial protein of unknown functionMitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication
1257YDR186CSND1Protein involved in SRP-independent targeting of substrates to the ERPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1258YDR187CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
1259YDR188WCCT6Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
1260YDR189WSLY1Hydrophilic protein involved in ER/Golgi vesicle traffickingHydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
1261YDR190CRVB1ATP-dependent DNA helicase, also known as pontinATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
1262YDR191WHST4NAD(+)-dependent protein deacetylaseMember of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
1263YDR192CNUP42FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
1264YDR193WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1265YDR194CMSS116Mitochondrial transcription elongation factorMitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate
1266YDR194W-AYDR194W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
1267YDR195WREF2RNA-binding proteinRNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; relocalizes to the cytosol in response to hypoxia
1268YDR196CCAB5Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC)Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets
1269YDR197WCBS2Mitochondrial translational activator of the COB mRNAMitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
1270YDR198CRKM2Ribosomal protein lysine methyltransferaseRibosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
1271YDR199WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64/YDR200C; computationally predicted to have thiol-disulfide oxidoreductase activity
1272YDR200CVPS64Protein required for cytoplasm to vacuole targeting of proteinsProtein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication
1273YDR201WSPC19Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
1274YDR202CRAV2Subunit of RAVE complex (Rav1p, Rav2p, Skp1p)Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
1275YDR203WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAV2/YDR202C
1276YDR204WCOQ4Protein with a role in ubiquinone (Coenzyme Q) biosynthesisProtein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
1277YDR205WMSC2Endoplasmic reticulum zinc transporterEndoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
1278YDR206WEBS1Protein involved in translation inhibition and nonsense-mediated decayProtein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication
1279YDR207CUME6Rpd3L histone deacetylase complex subunitRpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p
1280YDR208WMSS4Phosphatidylinositol-4-phosphate 5-kinasePhosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
1281YDR209CYDR209CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W
1282YDR210C-CYDR210C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1283YDR210C-DYDR210C-DRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1284YDR210WYDR210WPredicted tail-anchored plasma membrane proteinPredicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
1285YDR210W-AYDR210W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1286YDR210W-BYDR210W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1287YDR211WGCD6Catalytic epsilon subunit of the translation initiation factor eIF2BCatalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
1288YDR212WTCP1Alpha subunit of chaperonin-containing T-complexAlpha subunit of chaperonin-containing T-complex; complex mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
1289YDR213WUPC2Sterol regulatory element binding proteinSterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
1290YDR214WAHA1Co-chaperone that binds Hsp82p and activates its ATPase activityCo-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
1291YDR215CYDR215CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
1292YDR216WADR1Carbon source-responsive zinc-finger transcription factorCarbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
1293YDR217CRAD9DNA damage-dependent checkpoint proteinDNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
1294YDR218CSPR28Sporulation-specific homolog of the CDC3/10/11/12 family of genesSporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
1295YDR219CMFB1Mitochondria-associated F-box proteinMitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding
1296YDR220CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein
1297YDR221WGTB1Glucosidase II beta subunit, forms a complex with alpha subunit Rot2pGlucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
1298YDR222WYDR222WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication
1299YDR223WCRF1Transcriptional corepressorTranscriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication
1300YDR224CHTB1Histone H2BHistone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
1301YDR225WHTA1Histone H2AHistone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
1302YDR226WADK1Adenylate kinase, required for purine metabolismAdenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress
1303YDR227WSIR4SIR protein involved in assembly of silent chromatin domainsSIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan
1304YDR228CPCF11mRNA 3' end processing factormRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia
1305YDR229WIVY1Phospholipid-binding protein that interacts with both Ypt7p and Vps33pPhospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase
1306YDR230WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20/YDR231C
1307YDR231CCOX20Mitochondrial inner membrane proteinMitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
1308YDR232WHEM15-aminolevulinate synthase5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
1309YDR233CRTN1Reticulon proteinReticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication
1310YDR234WLYS4HomoaconitaseHomoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
1311YDR235WPRP42U1 snRNP protein involved in splicingU1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
1312YDR236CFMN1Riboflavin kinase, produces riboflavin monophosphate (FMN)Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
1313YDR237WMRPL7Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
1314YDR238CSEC26Essential beta-coat protein of the COPI coatomerEssential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
1315YDR239CYDR239CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments
1316YDR240CSNU56Component of U1 snRNP required for mRNA splicing via spliceosomeComponent of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
1317YDR241WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay
1318YDR242WAMD2Putative amidasePutative amidase
1319YDR243CPRP28RNA binding proteinRNA helicase in the DEAD-box family; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site
1320YDR244WPEX5Peroxisomal membrane signal receptor for peroxisomal matrix proteinsPeroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
1321YDR245WMNN10Subunit of a Golgi mannosyltransferase complexSubunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p
1322YDR246WTRS23Core component of transport protein particle (TRAPP) complexes I-IIICore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4
1323YDR246W-AYDR246W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
1324YDR247WVHS1Cytoplasmic serine/threonine protein kinaseCytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication
1325YDR248CYDR248CPutative gluconokinasePutative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
1326YDR249CYDR249CPutative protein of unknown functionPutative protein of unknown function
1327YDR250CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1328YDR251WPAM1Essential protein of unknown functionEssential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication
1329YDR252WBTT1Heterotrimeric nascent polypeptide-associated complex beta3 subunitHeterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication
1330YDR253CMET32Zinc-finger DNA-binding transcription factorZinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
1331YDR254WCHL4Outer kinetochore protein required for chromosome stabilityOuter kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
1332YDR255CRMD5Component of GID Complex that confers ubiquitin ligase (U3) activityComponent of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
1333YDR256CCTA1Catalase ACatalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation
1334YDR257CRKM4Ribosomal lysine methyltransferaseRibosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
1335YDR258CHSP78Oligomeric mitochondrial matrix chaperoneOligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates
1336YDR259CYAP6Basic leucine zipper (bZIP) transcription factorBasic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication
1337YDR260CSWM1Subunit of the anaphase-promoting complex (APC)Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
1338YDR261CEXG2Exo-1,3-beta-glucanaseExo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
1339YDR261C-CYDR261C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably non-functional
1340YDR261C-DYDR261C-DRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional
1341YDR261W-AYDR261W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1342YDR261W-BYDR261W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1343YDR262WYDR262WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment
1344YDR263CDIN7Mitochondrial nuclease functioning in DNA repair and replicationMitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication
1345YDR264CAKR1Palmitoyl transferase involved in protein palmitoylationPalmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
1346YDR265WPEX10Peroxisomal membrane E3 ubiquitin ligasePeroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders
1347YDR266CHEL2RING finger ubiquitin ligase (E3)RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
1348YDR267CCIA1Component of cytosolic iron-sulfur protein assembly (CIA) machineryComponent of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
1349YDR268WMSW1Mitochondrial tryptophanyl-tRNA synthetaseMitochondrial tryptophanyl-tRNA synthetase
1350YDR269CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1351YDR270WCCC2Cu(+2)-transporting P-type ATPaseCu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
1352YDR271CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
1353YDR272WGLO2Cytoplasmic glyoxalase IICytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication
1354YDR273WDON1Meiosis-specific component of the spindle pole bodyMeiosis-specific component of the spindle pole body; part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; DON1 has a paralog, CUE5, that arose from the whole genome
1355YDR274CYDR274CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1356YDR275WBSC2Protein of unknown functionProtein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
1357YDR276CPMP3Small plasma membrane proteinSmall plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential
1358YDR277CMTH1Negative regulator of the glucose-sensing signal transduction pathwayNegative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication
1359YDR278CYDR278CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1360YDR279WRNH202Ribonuclease H2 subunitRibonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome
1361YDR280WRRP45Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
1362YDR281CPHM6Protein of unknown functionProtein of unknown function; expression is regulated by phosphate levels
1363YDR282CMRX10Mitochondrial inner membrane protein of unknown functionMitochondrial inner membrane protein of unknown function; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation
1364YDR283CGCN2Protein kinaseProtein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
1365YDR284CDPP1Diacylglycerol pyrophosphate (DGPP) phosphataseDiacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
1366YDR285WZIP1Transverse filament protein of the synaptonemal complexTransverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-allelc sites; potential Cdc28p substrate
1367YDR286CMGP12Putative protein of unknown functionPutative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site
1368YDR287WINM2Inositol monophosphataseInositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
1369YDR288WNSE3Component of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
1370YDR289CRTT103Protein involved in transcription termination by RNA polymerase IIProtein involved in transcription termination by RNA polymerase II; interacts with exonuclease Rat1p and Rai1p; has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
1371YDR290WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
1372YDR291WHRQ13'-5' DNA helicase that has DNA strand annealing activity3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS)
1373YDR292CSRP101Signal recognition particle (SRP) receptor alpha subunitSignal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
1374YDR293CSSD1Translational repressor with a role in polar growth and wall integrityTranslational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
1375YDR294CDPL1Dihydrosphingosine phosphate lyaseDihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
1376YDR295CHDA2Subunit of the HDA1 histone deacetylase complexSubunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia
1377YDR296WMHR1Mitochondrial ribosomal protein of the large subunitProtein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
1378YDR297WSUR2Sphinganine C4-hydroxylaseSphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
1379YDR298CATP5Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthaseSubunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
1380YDR299WBFR2Component of the SSU and 90S preribosomesComponent of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
1381YDR300CPRO1Gamma-glutamyl kinaseGamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication
1382YDR301WCFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factorRNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
1383YDR302WGPI11ER membrane protein involved in a late step of GPI anchor assemblyER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog
1384YDR303CRSC3Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication
1385YDR304CCPR5Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ERPeptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication
1386YDR305CHNT2Dinucleoside triphosphate hydrolaseDinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
1387YDR306CYDR306CF-box protein of unknown functionF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
1388YDR307WPMT7Putative protein mannosyltransferase similar to Pmt1pPutative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation
1389YDR308CSRB7Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
1390YDR309CGIC2Redundant rho-like GTPase Cdc42p effectorRedundant rho-like GTPase Cdc42p effector; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; GIC2 has a paralog, GIC1, that arose from the whole genome duplication
1391YDR310CSUM1Transcriptional repressor that regulates middle-sporulation genesTranscriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
1392YDR311WTFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexesSubunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
1393YDR312WSSF2Protein required for ribosomal large subunit maturationProtein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
1394YDR313CPIB1RING-type ubiquitin ligase of the endosomal and vacuolar membranesRING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
1395YDR314CRAD34Protein involved in nucleotide excision repair (NER)Protein involved in nucleotide excision repair (NER); homologous to RAD4
1396YDR315CIPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinaseInositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
1397YDR316WOMS1Protein integral to the mitochondrial membraneProtein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
1398YDR316W-AYDR316W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1399YDR316W-BYDR316W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1400YDR317WHIM1Protein of unknown function involved in DNA repairProtein of unknown function involved in DNA repair
1401YDR318WMCM21Component of the kinetochore sub-complex COMAComponent of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
1402YDR319CYFT2Protein required for normal ER membrane biosynthesisProtein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
1403YDR320CSWA2Auxilin-like protein involved in vesicular transportAuxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
1404YDR320C-ADAD4Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
1405YDR320W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DAD4/YDR320C-A
1406YDR321WASP1Cytosolic L-asparaginase, involved in asparagine catabolismCytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively
1407YDR322C-ATIM11Subunit e of mitochondrial F1F0-ATPaseSubunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae
1408YDR322WMRPL35Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
1409YDR323CPEP7Adaptor protein involved in vesicle-mediated vacuolar protein sortingAdaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
1410YDR324CUTP4Subunit of U3-containing 90S preribosome and SSU processome complexesSubunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript; Small Subunit processome is also known as SSU processome
1411YDR325WYCG1Subunit of the condensin complexSubunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
1412YDR326CYSP2Sterol-binding proteinProtein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
1413YDR327WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SKP1
1414YDR328CSKP1Evolutionarily conserved kinetochore proteinEvolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
1415YDR329CPEX3Peroxisomal membrane protein (PMP)Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
1416YDR330WUBX5UBX domain-containing protein that interacts with Cdc48pUBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX
1417YDR331WGPI8ER membrane glycoprotein subunit of the GPI transamidase complexER membrane glycoprotein subunit of the GPI transamidase complex; adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
1418YDR332WIRC3Double-stranded DNA-dependent helicase of the DExH/D-box familyPutative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
1419YDR333CRQC1Component of the ribosome quality control complex (RQC)Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
1420YDR334WSWR1Swi2/Snf2-related ATPaseSwi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction
1421YDR335WMSN5KaryopherinKaryopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog
1422YDR336WMRX8Protein that associates with mitochondrial ribosomePutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene
1423YDR337WMRPS28Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
1424YDR338CYDR338CPutative protein of unknown functionPutative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
1425YDR339CFCF1Putative PINc domain nucleasePutative PINc domain nuclease; required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
1426YDR340WPutative protein of unknown function
1427YDR341CYDR341CArginyl-tRNA synthetaseArginyl-tRNA synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDR341C has a paralog, MSR1, that arose from the whole genome duplication
1428YDR342CHXT7High-affinity glucose transporterHigh-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels; HXT7 has a paralog, HXT4, that arose from the whole genome duplication
1429YDR343CHXT6High-affinity glucose transporterHigh-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3; HXT6 has a paralog, HXT1, that arose from the whole genome duplication
1430YDR344CYDR344CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1431YDR345CHXT3Low affinity glucose transporter of the major facilitator superfamilyLow affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
1432YDR346CSVF1Protein with a potential role in cell survival pathwaysProtein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
1433YDR347WMRP1Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
1434YDR348CPAL1Protein of unknown function thought to be involved in endocytosisProtein of unknown function thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication
1435YDR349CYPS7Putative GPI-anchored aspartic proteasePutative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
1436YDR350CATP22Specific translational activator for the mitochondrial ATP6 mRNASpecific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane
1437YDR351WSBE2Protein required for bud growthProtein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication
1438YDR352WYPQ2Putative vacuolar membrane transporter for cationic amino acidsPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
1439YDR353WTRR1Cytoplasmic thioredoxin reductaseCytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
1440YDR354C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TRP4; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1441YDR354WTRP4Anthranilate phosphoribosyl transferaseAnthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis
1442YDR355CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
1443YDR356WSPC110Inner plaque spindle pole body (SPB) componentInner plaque spindle pole body (SPB) component; ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
1444YDR357CCNL1Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
1445YDR358WGGA1Golgi-localized protein with homology to gamma-adaptinGolgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication
1446YDR359CEAF1Component of the NuA4 histone acetyltransferase complexComponent of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
1447YDR360WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C
1448YDR361CBCP1Essential protein involved in nuclear export of Mss4pEssential protein involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
1449YDR362CTFC6Subunit of RNA polymerase III transcription initiation factor complexSubunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
1450YDR363WESC2Sumo-like domain proteinSumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
1451YDR363W-ASEM119S proteasome regulatory particle lid subcomplex componentComponent of lid subcomplex of 26S proteasome regulatory subunit; involved in mRNA export mediated by TREX-2 complex (Sac3p-Thp1p); assumes different conformations in different contexts, functions as molecular glue stabilizing the Rpn3p/Rpn7p regulatory heterodimer, and tethers it to lid helical bundle; ortholog of human DSS1; protein abundance increases in response to DNA replication stress
1452YDR364CCDC40Pre-mRNA splicing factorPre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats
1453YDR365CESF1Nucleolar protein involved in pre-rRNA processingNucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
1454YDR365W-AYDR365W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1455YDR365W-BYDR365W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1456YDR366CYDR366CPutative protein of unknown functionPutative protein of unknown function
1457YDR367WKEI1Component of inositol phosphorylceramide (IPC) synthaseComponent of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
1458YDR368WYPR1NADPH-dependent aldo-keto reductaseNADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication
1459YDR369CXRS2Protein required for DNA repairProtein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
1460YDR370CDXO1mRNA 5'-end-capping quality-control proteinmRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p;
1461YDR371C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene CTS2/YDR371W; identified by expression profiling and mass spectrometry
1462YDR371WCTS2Putative chitinasePutative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect
1463YDR372CVPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensorGolgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
1464YDR373WFRQ1N-myristoylated calcium-binding proteinN-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
1465YDR374CPHO92Posttranscriptional regulator of phosphate metabolismPosttranscriptional regulator of phosphate metabolism; facilitates PHO4 mRNA degradation by interacting with Pop2p; regulates PHO4 mRNA stability by binding to PHO4's 3'UTR in a phosphate-dependent manner; contains highly conserved YTH (YT521-B Homology) domain that exhibits RNA-binding activity; functional homolog of human YTHDF2
1466YDR374W-AWIP1Kinetochore localized protein of unknown functionKinetochore localized protein of unknown function; interacts with Cnn1p (CENP-T); orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-W and fission yeast new1
1467YDR375CBCS1Protein translocase and chaperone required for Complex III assemblyProtein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
1468YDR376WARH1Oxidoreductase of the mitochondrial inner membraneOxidoreductase of the mitochondrial inner membrane; involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
1469YDR377WATP17Subunit f of the F0 sector of mitochondrial F1F0 ATP synthaseSubunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
1470YDR378CLSM6Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
1471YDR379C-ASDH6Mitochondrial protein involved in assembly of succinate dehydrogenaseMitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; member of the LYR protein family; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy
1472YDR379WRGA2GTPase-activating protein for polarity-establishment protein Cdc42pGTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication
1473YDR380WARO10Phenylpyruvate decarboxylasePhenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism
1474YDR381C-ACOI1Mitochondrial assembly factorProtein of unknown function; localized to the mitochondrial outer membrane
1475YDR381WYRA1Nuclear polyadenylated RNA-binding proteinNuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member
1476YDR382WRPP2BRibosomal protein P2 betaRibosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
1477YDR383CNKP1Central kinetochore protein and subunit of the Ctf19 complexCentral kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4
1478YDR384CATO3Plasma membrane protein, putative ammonium transporterPlasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
1479YDR385WEFT2Elongation factor 2 (EF-2), also encoded by EFT1Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
1480YDR386WMUS81Subunit of structure-specific Mms4p-Mus81p endonucleaseSubunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p
1481YDR387CCIN10Putative transporterPutative transporter; member of the sugar porter family; YDR387C is not an essential gene
1482YDR388WRVS167Calmodulin-binding actin-associated proteinActin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin
1483YDR389WSAC7GTPase activating protein (GAP) for Rho1pGTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication
1484YDR390CUBA2Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1pSubunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability;
1485YDR391CYDR391CPutative protein of unknown functionPutative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
1486YDR392WSPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexesSubunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia
1487YDR393WSHE9Protein required for normal mitochondrial morphologyProtein required for normal mitochondrial morphology; mitochondrial inner membrane protein; may be involved in fission of the inner membrane; forms a homo-oligomeric complex
1488YDR394WRPT3ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
1489YDR395WSXM1Nuclear transport factor (karyopherin)Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
1490YDR396WDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
1491YDR397CNCB2Subunit of a heterodimeric NC2 transcription regulator complexSubunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p
1492YDR398WUTP5Subunit of U3-containing Small Subunit (SSU) processome complexSubunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
1493YDR399WHPT1Dimeric hypoxanthine-guanine phosphoribosyltransferaseDimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
1494YDR400WURH1Uridine nucleosidase (uridine-cytidine N-ribohydrolase)Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
1495YDR401WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1496YDR402CDIT2N-formyltyrosine oxidaseN-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s
1497YDR403WDIT1Sporulation-specific enzyme required for spore wall maturationSporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
1498YDR404CRPB7RNA polymerase II subunit B16RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
1499YDR405WMRP20Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
1500YDR406WPDR15Plasma membrane ATP binding cassette (ABC) transporterPlasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication
1501YDR406W-ADubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR
1502YDR407CTRS120Component of transport protein particle (TRAPP) complex IIComponent of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
1503YDR408CADE8Phosphoribosyl-glycinamide transformylasePhosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
1504YDR409WSIZ1SUMO E3 ligaseSUMO/Smt3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring; SIZ1 has a paralog, NFI1, that arose from the whole genome duplication
1505YDR410CSTE14Farnesyl cysteine-carboxyl methyltransferaseFarnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
1506YDR411CDFM1Endoplasmic reticulum (ER) localized proteinEndoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
1507YDR412WRRP17Component of the pre-60S pre-ribosomal particleComponent of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
1508YDR413CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
1509YDR414CERD1Predicted membrane protein required for lumenal ER protein retentionPredicted membrane protein required for lumenal ER protein retention; mutants secrete the endogenous ER protein, BiP (Kar2p)
1510YDR415CYDR415CPutative aminopeptidasePutative aminopeptidase
1511YDR416WSYF1Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
1512YDR417CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
1513YDR418WRPL12BRibosomal 60S subunit protein L12BRibosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
1514YDR419WRAD30DNA polymerase etaDNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
1515YDR420WHKR1Mucin family member that functions as an osmosensor in the HOG pathwayMucin family member that functions as an osmosensor in the HOG pathway; functions in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
1516YDR421WARO80Zinc finger transcriptional activator of the Zn2Cys6 familyZinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
1517YDR422CSIP1Alternate beta-subunit of the Snf1p kinase complexAlternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions
1518YDR423CCAD1AP-1-like basic leucine zipper (bZIP) transcriptional activatorAP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
1519YDR424CDYN2Cytoplasmic light chain dynein, microtubule motor proteinCytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
1520YDR425WSNX41Sorting nexinSorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
1521YDR426CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41
1522YDR427WRPN9Non-ATPase regulatory subunit of the 26S proteasomeNon-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
1523YDR428CBNA7Formylkynurenine formamidaseFormylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
1524YDR429CTIF35eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3)eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
1525YDR430CCYM1Lysine-specific metalloprotease of the pitrilysin familyLysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology
1526YDR431WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1527YDR432WNPL3RNA-binding proteinRNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress
1528YDR433WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1529YDR434WGPI17Transmembrane proteinTransmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
1530YDR435CPPM1Carboxyl methyltransferaseCarboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth
1531YDR436WPPZ2Serine/threonine protein phosphatase Z, isoform of Ppz1pSerine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
1532YDR437WGPI19Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PISubunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P
1533YDR438WTHI74Mitochondrial transporter repressible by thiamineMitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension
1534YDR439WLRS4Nucleolar protein that forms a complex with Csm1pNucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
1535YDR440WDOT1Nucleosomal histone H3-Lys79 methylaseNucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
1536YDR441CAPT2Potential adenine phosphoribosyltransferasePotential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication
1537YDR442WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1538YDR443CSSN2Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
1539YDR444WYDR444WPutative hydrolase acting on ester bondsPutative hydrolase acting on ester bonds
1540YDR445CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1541YDR446WECM11Meiosis-specific proteinMeiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
1542YDR447CRPS17BRibosomal protein 51 (rp51) of the small (40s) subunitRibosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
1543YDR448WADA2Transcription coactivatorTranscription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
1544YDR449CUTP6Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
1545YDR450WRPS18AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
1546YDR451CYHP1Homeobox transcriptional repressorHomeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
1547YDR452WPPN1Dual endo- and exopolyphosphatase with a role in phosphate metabolismVacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
1548YDR453CTSA2Stress inducible cytoplasmic thioredoxin peroxidaseStress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication
1549YDR454CGUK1Guanylate kinaseGuanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
1550YDR455CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
1551YDR456WNHX1Na+/H+ and K+/H+ exchangerNa+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism
1552YDR457WTOM1E3 ubiquitin ligase of the hect-domain classE3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
1553YDR458CHEH2Inner nuclear membrane (INM) proteinInner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication
1554YDR459CPFA5Palmitoyltransferase with autoacylation activityPalmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
1555YDR460WTFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexesSubunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
1556YDR461C-ACMI8Putative protein of unknown functionPutative protein of unknown function
1557YDR461WMFA1Mating pheromone a-factorMating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2
1558YDR462WMRPL28Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
1559YDR463WSTP1Transcription factorTranscription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
1560YDR464C-ADubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1561YDR464WSPP41Protein of unknown functionProtein of unknown function; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia
1562YDR465CRMT2Arginine N5 methyltransferaseArginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
1563YDR466WPKH3Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2pProtein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
1564YDR467CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1565YDR468CTLG1Essential t-SNARE that mediates fusion of vesicles with the late GolgiEssential t-SNARE that mediates fusion of vesicles with the late Golgi; forms a complex with Tlg2p and Vti1p; mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
1566YDR469WSDC1Subunit of the COMPASS (Set1C) complexSubunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
1567YDR470CUGO1Outer membrane component of the mitochondrial fusion machineryOuter membrane component of the mitochondrial fusion machinery; binds directly to Fzo1p and Mgm1p and thus links these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but is not likely to function as a transmembrane transporter
1568YDR471WRPL27BRibosomal 60S subunit protein L27BRibosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
1569YDR472WTRS31Core component of transport protein particle (TRAPP) complexes I-IIICore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII)
1570YDR473CPRP3Splicing factorSplicing factor; component of the U4/U6-U5 snRNP complex
1571YDR475CJIP4Protein of unknown functionProtein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication
1572YDR476CYDR476CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
1573YDR477WSNF1AMP-activated S/T protein kinaseAMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ubiquitin ligase
1574YDR478WSNM1Ribonuclease MRP complex subunitRibonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
1575YDR479CPEX29ER-resident protein involved in peroxisomal biogenesisPeroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
1576YDR480WDIG2MAP kinase-responsive inhibitor of the Ste12p transcription factorMAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication
1577YDR481CPHO8Repressible vacuolar alkaline phosphataseRepressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
1578YDR482CCWC21Protein involved in RNA splicing by the spliceosomeProtein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
1579YDR483WKRE2Alpha1,2-mannosyltransferase of the GolgiAlpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
1580YDR484WVPS52Component of the GARP (Golgi-associated retrograde protein) complexComponent of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
1581YDR485CVPS72Htz1p-binding component of the SWR1 complexHtz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting
1582YDR486CVPS60Protein involved in late endosome to vacuole transportProtein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
1583YDR487CRIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase)3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase); required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
1584YDR488CPAC11Dynein intermediate chain, microtubule motor proteinDynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8
1585YDR489WSLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
1586YDR490CPKH1Serine/threonine protein kinaseSerine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication
1587YDR491CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1588YDR492WIZH1Membrane protein involved in zinc ion homeostasisMembrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication
1589YDR493WMZM1Protein required for assembly of the cytochrome bc(1) complexProtein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog
1590YDR494WRSM28Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation
1591YDR495CVPS3Component of CORVET membrane tethering complexComponent of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
1592YDR496CPUF6Pumilio-homology domain proteinPumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
1593YDR497CITR1Myo-inositol transporterMyo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication
1594YDR498CSEC20Membrane glycoprotein v-SNAREMembrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
1595YDR499WLCD1Essential protein required for the DNA integrity checkpoint pathwaysEssential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
1596YDR500CRPL37BRibosomal 60S subunit protein L37BRibosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
1597YDR501WPLM2Putative transcription factor, contains Forkhead Associated domainPutative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
1598YDR502CSAM2S-adenosylmethionine synthetaseS-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
1599YDR503CLPP1Lipid phosphate phosphataseLipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
1600YDR504CSPG3Protein required for high temperature survival during stationary phaseProtein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources
1601YDR505CPSP1Asn and gln rich protein of unknown functionAsn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
1602YDR506CGMC1Protein involved in meiotic progressionProtein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein
1603YDR507CGIN4Protein kinase involved in bud growth and assembly of the septin ringProtein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
1604YDR508CGNP1High-affinity glutamine permeaseHigh-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
1605YDR509WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1606YDR510C-ADubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1607YDR510WSMT3Ubiquitin-like protein of the SUMO familyUbiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
1608YDR511WSDH7Mitochondrial protein involved in assembly of succinate dehydrogenaseMitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family
1609YDR512CEMI1Non-essential protein of unknown functionNon-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs
1610YDR513WGRX2Cytoplasmic glutaredoxinCytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
1611YDR514CYDR514CProtein of unknown function that localizes to mitochondriaProtein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
1612YDR515WSLF1RNA binding protein that associates with polysomesRNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
1613YDR516CEMI2Non-essential protein of unknown functionNon-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
1614YDR517WGRH1Acetylated cis-Golgi protein, homolog of human GRASP65Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
1615YDR518WEUG1Protein disulfide isomerase of the endoplasmic reticulum lumenProtein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
1616YDR519WFPR2Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase)Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress
1617YDR520CURC2Putative Zn(II)2Cys6 motif containing transcription factorPutative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
1618YDR521WDubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
1619YDR522CSPS2Protein expressed during sporulationProtein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component
1620YDR523CSPS1Putative protein serine/threonine kinasePutative protein serine/threonine kinase; expressed at the end of meiosis and localized to the prospore membrane; required for correct localization of enzymes involved in spore wall synthesis
1621YDR524CAGE1ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effectorADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
1622YDR524C-ADubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1623YDR524C-BYDR524C-BPutative protein of unknown functionPutative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication
1624YDR524W-CYDR524W-CPutative protein of unknown functionPutative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin
1625YDR525WAPI2Putative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
1626YDR525W-ASNA2Protein of unknown functionProtein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
1627YDR526CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1628YDR527WRBA50Protein involved in transcriptionProtein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
1629YDR528WHLR1Protein involved in regulation of cell wall composition and integrityProtein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
1630YDR529CQCR7Subunit 7 of ubiquinol cytochrome-c reductase (Complex III)Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
1631YDR530CAPA2Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase IIDiadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
1632YDR531WCAB1Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferasePantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
1633YDR532CKRE28Subunit of a kinetochore-microtubule binding complexSubunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation
1634YDR533CHSP31Methylglyoxalase that converts methylglyoxal to D-lactateMethylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
1635YDR534CFIT1Mannoprotein that is incorporated into the cell wallMannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall
1636YDR535CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene
1637YDR536WSTL1Glycerol proton symporter of the plasma membraneGlycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
1638YDR537CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF PAD1/YDR538W
1639YDR538WPAD1Phenylacrylic acid decarboxylasePhenylacrylic acid decarboxylase; confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
1640YDR539WFDC1Ferulic acid decarboxylasePutative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
1641YDR540CIRC4Protein of unknown functionPutative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
1642YDR541CYDR541CAldehyde reductasePutative dihydrokaempferol 4-reductase
1643YDR542WPAU10Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
1644YDR543CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1645YDR544CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1646YDR545C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-1/YDR545W
1647YDR545WYRF1-1Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
1648YEL001CIRC22Protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
1649YEL002CWBP1Beta subunit of the oligosaccharyl transferase glycoprotein complexBeta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
1650YEL003WGIM4Subunit of the heterohexameric cochaperone prefoldin complexSubunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
1651YEL004WYEA4Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporterUridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER
1652YEL005CVAB2Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern
1653YEL006WYEA6Putative mitochondrial NAD+ transporterPutative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication
1654YEL007WMIT1Transcriptional regulator of pseudohyphal growthTranscriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
1655YEL008C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1656YEL008WYEL008WPutative protein of unknown functionHypothetical protein predicted to be involved in metabolism
1657YEL009CGCN4bZIP transcriptional activator of amino acid biosynthetic genesbZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
1658YEL009C-AYEL009C-APutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YEL010W
1659YEL010WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1660YEL011WGLC3Glycogen branching enzyme, involved in glycogen accumulationGlycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
1661YEL012WUBC8Ubiquitin-conjugating enzyme that regulates gluconeogenesisUbiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
1662YEL013WVAC8Vacuolar membrane proteinPhosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
1663YEL014CYEL014CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1664YEL015WEDC3Non-essential conserved protein with a role in mRNA decappingNon-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress
1665YEL016CNPP2Nucleotide pyrophosphatase/phosphodiesteraseNucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP2 has a paralog, NPP1, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants
1666YEL017C-APMP2Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p)Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
1667YEL017WGTT3Protein of unknown function may be involved in glutathione metabolismProtein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
1668YEL018C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF EAF5/YEL018W
1669YEL018WEAF5Non-essential subunit of the NuA4 acetyltransferase complexNon-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia
1670YEL019CMMS21Highly conserved SUMO E3 ligase subunit of SMC5-SMC6 complexSUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; required for efficient sister chromatid cohesion; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function
1671YEL020CPXP1Peroxisomal matrix proteinProtein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data
1672YEL020C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YEL020W-A; identified by fungal homology and RT-PCR
1673YEL020W-ATIM9Essential protein of the mitochondrial intermembrane spaceEssential protein of the mitochondrial intermembrane space; forms a complex with Tim10p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
1674YEL021WURA3Orotidine-5'-phosphate (OMP) decarboxylaseOrotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound
1675YEL022WGEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs)Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
1676YEL023CYEL023CPutative protein of unknown functionPutative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
1677YEL024WRIP1Ubiquinol-cytochrome-c reductaseUbiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
1678YEL025CYEL025CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
1679YEL026WSNU13RNA binding proteinRNA binding protein; part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
1680YEL027WVMA3Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPaseProteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis
1681YEL028WYEL028WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1682YEL029CBUD16Putative pyridoxal kinasePutative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
1683YEL030C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1684YEL030WECM10Heat shock protein of the Hsp70 familyHeat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication
1685YEL031WSPF1P-type ATPase, ion transporter of the ER membraneP-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
1686YEL032C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1687YEL032WMCM3Protein involved in DNA replicationProtein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex
1688YEL033WMTC7Protein of unknown functionProtein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
1689YEL034C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps HYP2/YEL034W, a verified gene that encodes eiF-5A
1690YEL034WHYP2Translation elongation factor eIF-5ATranslation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
1691YEL035CUTR5Protein of unknown functionProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
1692YEL036CANP1Subunit of the alpha-1,6 mannosyltransferase complexSubunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
1693YEL037CRAD23Protein with ubiquitin-like N terminusProtein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
1694YEL038WUTR4Protein with sequence similarity to acireductone synthasesProtein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus
1695YEL039CCYC7Cytochrome c isoform 2, expressed under hypoxic conditionsCytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
1696YEL040WUTR2Chitin transglycosylaseChitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
1697YEL041WYEF1ATP-NADH kinaseATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
1698YEL042WGDA1Guanosine diphosphatase located in the GolgiGuanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
1699YEL043WYEL043WPredicted cytoskeleton protein involved in intracellular signalingPredicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
1700YEL044WIES6Component of the INO80 chromatin remodeling complexComponent of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
1701YEL045CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress
1702YEL046CGLY1Threonine aldolaseThreonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
1703YEL047CFRD1Soluble fumarate reductaseSoluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
1704YEL048CTCA17Component of transport protein particle (TRAPP) complex IIComponent of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder
1705YEL049WPAU2Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
1706YEL050CRML2Mitochondrial ribosomal protein of the large subunit (L2)Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor
1707YEL050W-ADubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR
1708YEL051WVMA8Subunit D of the V1 peripheral membrane domain of V-ATPaseSubunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
1709YEL052WAFG1Protein that may act as a chaperone for cytochrome c oxidase subunitsProtein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain
1710YEL053CMAK10Non-catalytic subunit of the NatC N-terminal acetyltransferaseNon-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; expression is glucose-repressible
1711YEL053W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RPL12A/YEL054C
1712YEL054CRPL12ARibosomal 60S subunit protein L12ARibosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
1713YEL055CPOL5DNA Polymerase phiDNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
1714YEL056WHAT2Subunit of the Hat1p-Hat2p histone acetyltransferase complexSubunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
1715YEL057CSDD1Protein of unknown functionProtein of unknown function involved in telomere maintenance; target of UME6 regulation
1716YEL058WPCM1Essential N-acetylglucosamine-phosphate mutaseEssential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
1717YEL059C-ASOM1Subunit of the mitochondrial inner membrane peptidase (IMP)Subunit of the mitochondrial inner membrane peptidase (IMP); IMP is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
1718YEL059WDubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking
1719YEL060CPRB1Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activityVacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication
1720YEL061CCIN8Kinesin motor proteinKinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
1721YEL062WNPR2Subunit of the Iml1p/SEACIT complexSubunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human tumor suppressor NPRL2; target of Grr1p; required for growth on urea and proline
1722YEL063CCAN1Plasma membrane arginine permeasePlasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
1723YEL064CAVT2Putative transporterPutative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
1724YEL065WSIT1Ferrioxamine B transporterFerrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
1725YEL066WHPA3D-Amino acid N-acetyltransferase that detoxifies D-amino acidsD-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates
1726YEL067CYEL067CPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
1727YEL068CYEL068CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
1728YEL069CHXT13Putative transmembrane polyol transporterHexose transporter; induced in the presence of non-fermentable carbon sources, induced by low levels of glucose, repressed by high levels of glucose; HXT13 has a paralog, HXT17, that arose from a segmental duplication
1729YEL070WDSF1Mannitol dehydrogenasePutative mannitol dehydrogenase; deletion suppressor of mpt5 mutation; DSF1 has a paralog, YNR073C, that arose from a segmental duplication
1730YEL071WDLD32-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenaseD-lactate dehydrogenase; part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
1731YEL072WRMD6Protein required for sporulationProtein required for sporulation
1732YEL073CYEL073CPutative protein of unknown functionPutative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline
1733YEL074WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large scale expression analysis showed an increase in mRNA accumulation in strains mutant for exosome components
1734YEL075CYEL075CPutative protein of unknown functionPutative protein of unknown function
1735YEL075W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1736YEL076CYEL076CPutative protein of unknown functionPutative protein of unknown function
1737YEL076C-AYEL076C-APutative protein of unknown functionPutative protein of unknown function
1738YEL077CYEL077CHelicase-like protein encoded within the telomeric Y' elementHelicase-like protein encoded within the telomeric Y' element
1739YEL077W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1740YER001WMNN1Alpha-1,3-mannosyltransferaseAlpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
1741YER002WNOP16Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
1742YER003CPMI40Mannose-6-phosphate isomeraseMannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
1743YER004WFMP52Protein of unknown functionProtein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
1744YER005WYND1Apyrase with wide substrate specificityApyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
1745YER006C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PAC2/YER007W
1746YER006WNUG1GTPase that associates with nuclear 60S pre-ribosomesGTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus
1747YER007C-ATMA20Protein of unknown function that associates with ribosomesProtein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
1748YER007WPAC2Microtubule effector required for tubulin heterodimer formationMicrotubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
1749YER008CSEC3Subunit of the exocyst complexSubunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
1750YER009WNTF2Nuclear envelope proteinNuclear envelope protein; interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
1751YER010CYER010CBifunctional HMG aldolase/oxaloacetate decarboxylaseBifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate
1752YER011WTIR1Cell wall mannoproteinCell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
1753YER012WPRE1Beta 4 subunit of the 20S proteasomeBeta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
1754YER013WPRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicaseDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
1755YER014C-ABUD25Protein involved in bud-site selectionProtein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
1756YER014WHEM14Protoporphyrinogen oxidaseProtoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
1757YER015WFAA2Medium chain fatty acyl-CoA synthetaseMedium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome
1758YER016WBIM1Microtubule plus end-tracking proteinMicrotubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
1759YER017CAFG3Mitochondrial inner membrane m-AAA protease componentMitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging
1760YER018CSPC25Component of the kinetochore-associated Ndc80 complexComponent of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
1761YER019C-ASBH2Ssh1p-Sss1p-Sbh2p complex componentSsh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication
1762YER019WISC1Inositol phosphosphingolipid phospholipase CInositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance
1763YER020WGPA2Nucleotide binding alpha subunit of the heterotrimeric G proteinNucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus
1764YER021WRPN3Essential non-ATPase regulatory subunit of the 26S proteasome lidEssential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
1765YER022WSRB4Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
1766YER023C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene PRO3; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
1767YER023WPRO3Delta 1-pyrroline-5-carboxylate reductaseDelta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis
1768YER024WYAT2Carnitine acetyltransferaseCarnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
1769YER025WGCD11Gamma subunit of the translation initiation factor eIF2Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID)
1770YER026CCHO1Phosphatidylserine synthasePhosphatidylserine synthase; functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline
1771YER027CGAL83One of three possible beta-subunits of the Snf1 kinase complexOne of three possible beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain; GAL83 has a paralog, SIP2, that arose from the whole genome duplication
1772YER028CMIG3Transcriptional regulatorTranscriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genesenvironment
1773YER029CSMB1Core Sm protein Sm BCore Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B'
1774YER030WCHZ1Histone chaperone for Htz1p/H2A-H2B dimerHistone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
1775YER031CYPT31Rab family GTPaseRab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
1776YER032WFIR1Protein involved in 3' mRNA processingProtein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate
1777YER033CZRG8Protein of unknown functionProtein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
1778YER034WYER034WProtein of unknown functionProtein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
1779YER035WEDC2RNA-binding protein that directly activates mRNA decappingRNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication
1780YER036CARB1ATPase of the ATP-binding cassette (ABC) familyATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
1781YER037WPHM8Lysophosphatidic acid (LPA) phosphatase, nucleotidaseLysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; PHM8 has a paralog, SDT1, that arose from the whole genome duplication
1782YER038CKRE29Subunit of the SMC5-SMC6 complexSubunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
1783YER038W-AFMP49Mitochondrial protein of unknown functionMitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C
1784YER039CHVG1Protein of unknown functionProtein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication
1785YER039C-AYER039C-APutative protein of unknown functionPutative protein of unknown function; YER039C-A is not an essential gene
1786YER040WGLN3Transcriptional activator of genes regulated by nitrogen catabolite rTranscriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p
1787YER041WYEN1Holliday junction resolvaseHolliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p
1788YER042WMXR1Methionine-S-sulfoxide reductaseMethionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease
1789YER043CSAH1S-adenosyl-L-homocysteine hydrolaseS-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels
1790YER044CERG28Endoplasmic reticulum membrane proteinEndoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
1791YER044C-AMEI4Meiosis-specific protein involved in forming DSBsMeiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores
1792YER045CACA1ATF/CREB family basic leucine zipper (bZIP) transcription factorATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
1793YER046WSPO73Meiosis-specific protein required for prospore membrane morphogenesisMeiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
1794YER046W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SAP1/YER047C
1795YER047CSAP1Putative ATPase of the AAA familyPutative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system
1796YER048CCAJ1Nuclear type II J heat shock protein of the E. coli dnaJ familyNuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
1797YER048W-AISD11Cysteine desulfurase (Nfs1p) activatorCysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family
1798YER049WTPA1Fe(II)/2-oxoglutarate-dependent dioxygenase family memberPoly(rA)-binding protein; involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(III) and 2-oxoglutarate
1799YER050CRSM18Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein
1800YER051WJHD1JmjC domain family histone demethylase specific for H3-K36JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe
1801YER052CHOM3Aspartate kinase (L-aspartate 4-P-transferase)Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
1802YER053CPIC2Mitochondrial copper and phosphate carrierMitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
1803YER053C-AYER053C-AProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
1804YER054CGIP2Putative regulatory subunit of protein phosphatase Glc7pPutative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication
1805YER055CHIS1ATP phosphoribosyltransferaseATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
1806YER056CFCY2Purine-cytosine permeasePurine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
1807YER056C-ARPL34ARibosomal 60S subunit protein L34ARibosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
1808YER057CHMF1Member of the p14.5 protein familyMember of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication
1809YER058WPET117Assembly factor that couples heme a synthesis to complex IV assemblyProtein required for assembly of cytochrome c oxidase
1810YER059WPCL6Pho85p cyclin of the Pho80p subfamilyPho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication
1811YER060WFCY21Putative purine-cytosine permeasePutative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function
1812YER060W-AFCY22Putative purine-cytosine permeasePutative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function
1813YER061CCEM1Mitochondrial beta-keto-acyl synthaseMitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration
1814YER062CGPP2DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesisDL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication
1815YER063WTHO1Conserved nuclear RNA-binding proteinConserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
1816YER064CVHR2Non-essential nuclear proteinNon-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
1817YER065CICL1Isocitrate lyaseIsocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
1818YER066C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YER067W
1819YER066WRRT13Putative protein of unknown functionPutative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
1820YER067C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF RGI1/YER067W
1821YER067WRGI1Protein of unknown functionProtein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication
1822YER068C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARG5,6/YER069W
1823YER068WMOT2Ubiquitin-protein ligase subunit of the CCR4-NOT complexUbiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication
1824YER069WARG5,6Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductaseAcetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
1825YER070WRNR1Major isoform of large subunit of ribonucleotide-diphosphate reductaseMajor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication
1826YER071CTDA2Subunit of a complex that associates with actin filamentsProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele
1827YER072WVTC1Regulatory subunit of the vacuolar transporter chaperone (VTC) complexSubunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
1828YER073WALD5Mitochondrial aldehyde dehydrogenaseMitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
1829YER074WRPS24AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
1830YER074W-AYOS1Integral membrane protein required for ER to Golgi transportIntegral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p
1831YER075CPTP3Phosphotyrosine-specific protein phosphatasePhosphotyrosine-specific protein phosphatase; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm
1832YER076CYER076CPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
1833YER076W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER076C
1834YER077CMRX1Protein that associates with mitochondrial ribosomePutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
1835YER078CICP55Mitochondrial aminopeptidaseMitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family
1836YER078W-AYER078W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
1837YER079C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF AIM9/YER080W
1838YER079WYER079WPutative protein of unknown functionPutative protein of unknown function
1839YER080WAIM9Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
1840YER081WSER33-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication
1841YER082CUTP7Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
1842YER083CGET2Subunit of the GET complexSubunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
1843YER084WYER084WProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
1844YER084W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps uncharacterized ORF YER085C
1845YER085CYER085CPutative protein of unknown functionPutative protein of unknown function
1846YER086WILV1Threonine deaminase, catalyzes first step in isoleucine biosynthesisThreonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
1847YER087C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W
1848YER087C-BSBH1Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p)Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; cotranslationally N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose from the whole genome duplication
1849YER087WAIM10Protein with similarity to tRNA synthetasesProtein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
1850YER088CDOT6Protein involved in rRNA and ribosome biogenesisProtein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress
1851YER088C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs PTC2/YER089C and YER088W-B
1852YER088W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ORF PTC2/YER089C; identified by gene-trapping, expression analysis, and homology searching
1853YER089CPTC2Type 2C protein phosphatase (PP2C)Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
1854YER090C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1855YER090WTRP2Anthranilate synthaseAnthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
1856YER091CMET6Cobalamin-independent methionine synthaseCobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
1857YER091C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1858YER092WIES5Non-essential INO80 chromatin remodeling complex subunitNon-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination
1859YER093CTSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p)Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p); TORC2 is a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
1860YER093C-AAIM11Protein of unknown functionProtein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
1861YER094CPUP3Beta 3 subunit of the 20S proteasomeBeta 3 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit C10
1862YER095WRAD51Strand exchange proteinStrand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
1863YER096WSHC1Sporulation-specific activator of Chs3p (chitin synthase III)Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
1864YER097WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
1865YER098WUBP9Ubiquitin-specific protease that cleaves ubiquitin-protein fusionsUbiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
1866YER099CPRS25-phospho-ribosyl-1(alpha)-pyrophosphate synthetase5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
1867YER100WUBC6Ubiquitin-conjugating enzyme involved in ERADUbiquitin-conjugating enzyme involved in ERAD; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; ER-associated protein degradation is also known as ERAD
1868YER101CAST2Lipid raft associated proteinLipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
1869YER102WRPS8BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8B has a paralog, RPS8A, that arose from the whole genome duplication
1870YER103WSSA4Heat shock protein that is highly induced upon stressHeat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
1871YER104WRTT105Protein with a role in regulation of Ty1 transpositionProtein with a role in regulation of Ty1 transposition
1872YER105CNUP157Subunit of the inner ring of the nuclear pore complex (NPC)Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
1873YER106WMAM1MonopolinMonopolin; kinetochore associated protein involved in chromosome attachment to meiotic spindle
1874YER107CGLE2RNA export factor associated with the nuclear pore complex (NPC)RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
1875YER107W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF GLE2/YER107C
1876YER109CFLO8Transcription factorTranscription factor; required for flocculation, diploid filamentous growth, and haploid invasive growth; forms a heterodimer with Mss1p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1; genome reference strain S288C and most laboratory strains have a nonsense mutation in this gene
1877YER110CKAP123Karyopherin betaKaryopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
1878YER111CSWI4DNA binding component of the SBF complex (Swi4p-Swi6p)DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
1879YER112WLSM4Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
1880YER113CTMN3Protein with a role in cellular adhesion and filamentous growthProtein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
1881YER114CBOI2Protein implicated in polar growth, functionally redundant with Boi1pProtein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
1882YER115CSPR6Protein of unknown functionProtein of unknown function; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
1883YER116CSLX8Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complexSubunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress
1884YER117WRPL23BRibosomal 60S subunit protein L23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
1885YER118CSHO1Transmembrane osmosensor for filamentous growth and HOG pathwaysTransmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
1886YER119CAVT6Vacuolar aspartate and glutamate exporterVacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication
1887YER119C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
1888YER120WSCS2Integral ER membrane protein, regulates phospholipid metabolismIntegral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
1889YER121WYER121WPutative protein of unknown functionPutative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy
1890YER122CGLO3ADP-ribosylation factor GTPase activating protein (ARF GAP)ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p
1891YER123WYCK3Palmitoylated vacuolar membrane-localized casein kinase I isoformPalmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
1892YER124CDSE1Daughter cell-specific proteinDaughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
1893YER125WRSP5NEDD4 family E3 ubiquitin ligaseE3 ubiquitin ligase of NEDD4 family; regulates many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, ribosome stability; mutant tolerates aneuploidy; autoubiquitinates; ubiquitinates Sec23p and Sna3p; deubiquitinated by Ubp2p; activity regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; human homolog implicated in Liddle syndrome
1894YER126CNSA2Protein constituent of 66S pre-ribosomal particlesProtein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA
1895YER127WLCP5Essential protein involved in maturation of 18S rRNAEssential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
1896YER128WVFA1Protein that interacts with Vps4p and has a role in vacuolar sortingProtein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
1897YER129WSAK1Upstream serine/threonine kinase for the SNF1 complexUpstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
1898YER130CCOM2Transcription factor that binds IME1 Upstream Activation Signal (UAS)Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under all growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress
1899YER131WRPS26BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication
1900YER132CPMD1Protein with an N-terminal kelch-like domainProtein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication
1901YER133WGLC7Type 1 S/T protein phosphatase (PP1) catalytic subunitType 1 serine/threonine protein phosphatase catalytic subunit; cleavage and polyadenylation factor (CPF) component; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
1902YER133W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF YER134C
1903YER134CYER134CMagnesium-dependent acid phosphataseMagnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene
1904YER135CYER135CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YER135C is not an essential gene
1905YER136WGDI1GDP dissociation inhibitorGDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
1906YER137CYER137CPutative protein of unknown functionPutative protein of unknown function
1907YER137C-AYER137C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1908YER137W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YER137C
1909YER138CYER138CRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1910YER138W-AYER138W-APutative protein of unknown functionPutative protein of unknown function; YER138W-A has a paralog, YBL107W-A, that arose from a single-locus duplication
1911YER139CRTR1CTD phosphataseCTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
1912YER140WEMP65Integral membrane protein of the ERER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
1913YER141WCOX15Heme a synthaseProtein required for the hydroxylation of heme O to form heme A; heme A is an essential prosthetic group for cytochrome c oxidase
1914YER142CMAG13-methyl-adenine DNA glycosylase3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
1915YER143WDDI1DNA damage-inducible v-SNARE binding proteinDNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains
1916YER144CUBP5Putative ubiquitin-specific proteasePutative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication
1917YER145CFTR1High affinity iron permeaseHigh affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
1918YER145C-AYER145C-APutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF LSM5/YER146W
1919YER146WLSM5Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
1920YER147CSCC4Subunit of cohesin loading factor (Scc2p-Scc4p)Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
1921YER147C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SPT15/YER148W
1922YER148WSPT15TATA-binding protein (TBP)TATA-binding protein; general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
1923YER148W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF PEA2/YER149C
1924YER149CPEA2Coiled-coil 12S polarisome subunitCoiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion
1925YER150WSPI1GPI-anchored cell wall protein involved in weak acid resistanceGPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication
1926YER151CUBP3Ubiquitin-specific protease involved in transport and osmotic responseUbiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress; role in ribophagy
1927YER152CYER152CProtein with 2-aminoadipate transaminase activityProtein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
1928YER152W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YER152C
1929YER153CPET122Mitochondrial translational activator specific for the COX3 mRNAMitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
1930YER154WOXA1Mitochondrial inner membrane insertaseMitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
1931YER155CBEM2Rho GTPase activating protein (RhoGAP)Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p
1932YER156CYER156CPutative protein of unknown functionPutative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
1933YER157WCOG3Essential component of the conserved oligomeric Golgi complexEssential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
1934YER158CYER158CProtein of unknown functionProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
1935YER158W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1936YER159CBUR6Subunit of a heterodimeric NC2 transcription regulator complexSubunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p
1937YER159C-AYER159C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1938YER160CYER160CRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1939YER161CSPT2Protein involved in negative regulation of transcriptionProtein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins
1940YER162CRAD4Protein that recognizes and binds damaged DNA (with Rad23p) during NERProtein that recognizes and binds damaged DNA (with Rad23p) during NER; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins; NER stands for nucleotide excision repair
1941YER163CGCG1Gamma-glutamyl cyclotransferaseGamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
1942YER164WCHD1Chromatin remodeler that regulates various aspects of transcriptionChromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
1943YER165C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DNF1/YER166W
1944YER165WPAB1Poly(A) binding proteinPoly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex
1945YER166WDNF1Aminophospholipid translocase (flippase)Aminophospholipid translocase (flippase); localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
1946YER167WBCK2Serine/threonine-rich protein involved in PKC1 signaling pathwaySerine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations
1947YER168CCCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferaseATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
1948YER169WRPH1JmjC domain-containing histone demethylaseJmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNAP II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; target of stess-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
1949YER170WADK2Mitochondrial adenylate kinaseMitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
1950YER171WRAD35' to 3' DNA helicase5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
1951YER172CBRR2RNA-dependent ATPase RNA helicase (DEIH box)RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
1952YER172C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAD24/YER173W
1953YER173WRAD24Checkpoint proteinCheckpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
1954YER174CGRX4Glutathione-dependent oxidoreductaseGlutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication
1955YER175CTMT1Trans-aconitate methyltransferaseTrans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
1956YER175W-AYER175W-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1957YER176WECM32DNA dependent ATPase/DNA helicaseDNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
1958YER177WBMH114-3-3 protein, major isoform14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
1959YER178WPDA1E1 alpha subunit of the pyruvate dehydrogenase (PDH) complexE1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
1960YER179WDMC1Meiosis-specific recombinase required for double-strand break repairMeiosis-specific recombinase required for repair of double-strand breaks; also required for pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
1961YER180CISC10Protein required for sporulationProtein required for sporulation; transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
1962YER180C-ASLO1Protein interacting with Arl3pProtein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
1963YER181CYER181CMitochondrial protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies
1964YER182WFMP10Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
1965YER183CFAU15,10-methenyltetrahydrofolate synthetase5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
1966YER184CTOG1Transcriptional activator of oleate genesTranscriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source
1967YER185WPUG1Plasma membrane protein involved in protoprophyrin and heme transportPlasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
1968YER186CYER186CPutative protein of unknown functionPutative protein of unknown function
1969YER187WYER187WPutative protein of unknown functionPutative protein of unknown function; induced in respiratory-deficient cells
1970YER188C-AYER188C-APutative protein of unknown functionPutative protein of unknown function
1971YER188WYER188WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p
1972YER189WYER189WPutative protein of unknown functionPutative protein of unknown function
1973YER190C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-2/YER190W; identified by gene-trapping, expression analysis, and homology
1974YER190C-BYER190C-BPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
1975YER190WYRF1-2Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p; induced by treatment with 8-methoxypsoralen and UVA irradiation
1976YFL001WDEG1tRNA:pseudouridine synthasetRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
1977YFL002CSPB4Putative ATP-dependent RNA helicasePutative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
1978YFL002W-AYFL002W-ARetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
1979YFL002W-BYFL002W-BRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
1980YFL003CMSH4Protein involved in meiotic recombinationProtein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
1981YFL004WVTC2Regulatory subunit of the vacuolar transporter chaperone (VTC) complexSubunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication
1982YFL005WSEC4Rab family GTPaseRab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
1983YFL007WBLM10Proteasome activatorProteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
1984YFL008WSMC1Subunit of the multiprotein cohesin complexSubunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
1985YFL009WCDC4F-box protein required for both the G1/S and G2/M phase transitionsF-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p
1986YFL010CWWM1WW domain containing protein of unknown functionWW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
1987YFL010W-AAUA1Protein required for the negative regulation by ammonia of Gap1pProtein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease
1988YFL011WHXT10Putative hexose transporterPutative hexose transporter; expressed at low levels and expression is repressed by glucose
1989YFL012WYFL012WPutative protein of unknown functionPutative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin
1990YFL012W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene IES1/YFL013C
1991YFL013CIES1Subunit of the INO80 chromatin remodeling complexSubunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia
1992YFL013W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
1993YFL014WHSP12Plasma membrane protein involved in maintaining membrane organizationPlasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
1994YFL015CYFL015CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
1995YFL015W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YFL015C
1996YFL016CMDJ1Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activityCo-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
1997YFL017CGNA1Glucosamine-6-phosphate acetyltransferaseGlucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
1998YFL017W-ASMX2Core Sm protein Sm GCore Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
1999YFL018CLPD1Dihydrolipoamide dehydrogenaseDihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication
2000YFL019CYFL019CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YFL019C is not an essential gene
2001YFL020CPAU5Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme
2002YFL021C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF GAT1/YFL021W
2003YFL021WGAT1Transcriptional activator of nitrogen catabolite repression genesTranscriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized
2004YFL022CFRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetaseAlpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
2005YFL023WBUD27Unconventional prefoldin protein involved in translation initiationUnconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
2006YFL024CEPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complexSubunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb; coding sequence contains length polymorphisms in different strains
2007YFL025CBST1GPI inositol deacylase of the endoplasmic reticulum (ER)GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy
2008YFL026WSTE2Receptor for alpha-factor pheromoneReceptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
2009YFL027CGYP8GTPase-activating protein for yeast Rab family membersGTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
2010YFL028CCAF16Part of evolutionarily-conserved CCR4-NOT regulatory complexPart of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
2011YFL029CCAK1Cyclin-dependent kinase-activating kinaseCyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
2012YFL030WAGX1Alanine:glyoxylate aminotransferase (AGT)Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases
2013YFL031C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps HAC1/YFL031W; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2014YFL031WHAC1Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog)Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
2015YFL032WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
2016YFL033CRIM15Protein kinase involved in cell proliferation in response to nutrientsProtein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase
2017YFL034C-ARPL22BRibosomal 60S subunit protein L22ARibosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
2018YFL034C-BMOB2Activator of Cbk1p kinaseActivator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
2019YFL034WMIL1Predicted lipasePutative integral membrane protein that interacts with Rpp0p; Rpp0p is a component of the ribosomal stalk
2020YFL036WRPO41Mitochondrial RNA polymeraseMitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
2021YFL037WTUB2Beta-tubulinBeta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
2022YFL038CYPT1Rab family GTPaseRab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates unfolded protein response (UPR) by promoting the decay of HAC1 RNA
2023YFL039CACT1ActinActin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
2024YFL040WYFL040WPutative transporterPutative transporter; member of the sugar porter family; YFL040W is not an essential gene
2025YFL041WFET5Multicopper oxidaseMulticopper oxidase; integral membrane protein with similarity to Fet3p; may have a role in iron transport
2026YFL041W-AYFL041W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
2027YFL042CLAM5Putative sterol transfer proteinPutative protein of unknown function; not an essential gene; YFL042C has a paralog, YLR072W, that arose from the whole genome duplication
2028YFL044COTU1Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48pDeubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
2029YFL045CSEC53PhosphomannomutasePhosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
2030YFL046WFMP32Putative assembly factor for cytochrome c oxidasePutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2031YFL047WRGD2GTPase-activating protein (RhoGAP) for Cdc42p and Rho5pGTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
2032YFL048CEMP47Integral membrane component of ER-derived COPII-coated vesiclesIntegral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication
2033YFL049WSWP82Member of the SWI/SNF chromatin remodeling complexMember of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
2034YFL050CALR2Probable Mg(2+) transporterProbable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition
2035YFL051CYFL051CPutative protein of unknown functionPutative protein of unknown function; YFL051C is not an essential gene
2036YFL052WZNF1Zinc cluster transcription factor that regulates respiratory growthPutative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity
2037YFL053WDAK2Dihydroxyacetone kinaseDihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
2038YFL054CAQY3Putative channel-like proteinPutative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol
2039YFL055WAGP3Low-affinity amino acid permeaseLow-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
2040YFL056CAAD6Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family; expression induced in cells treated with the mycotoxin patulin
2041YFL057CAAD16Putative aryl alcohol dehydrogenasePutative aryl alcohol dehydrogenase; similar to Phanerochaete chrysosporium aryl alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
2042YFL058WTHI5Protein involved in synthesis of the thiamine precursor HMPProtein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
2043YFL059WSNZ3Member of a stationary phase-induced gene familyMember of a stationary phase-induced gene family; expressed in the presence of galactose; transcription of SNZ3 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3
2044YFL060CSNO3Protein of unknown functionProtein of unknown function; nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin
2045YFL061WDDI2Cyanamide hydratase that detoxifies cyanamideProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
2046YFL062WCOS4Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
2047YFL063WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2048YFL064CYFL064CPutative protein of unknown functionPutative protein of unknown function
2049YFL065CYFL065CPutative protein of unknown functionPutative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation
2050YFL066CYFL066CHelicase-like protein encoded within the telomeric Y' elementHelicase-like protein encoded within the telomeric Y' element; induced by treatment with 8-methoxypsoralen and UVA irradiation
2051YFL067WYFL067WProtein of unknown functionProtein of unknown function; down-regulated at low calcium levels
2052YFL068WYFL068WPutative protein of unknown functionPutative protein of unknown function
2053YFR001WLOC1Nucleolar protein involved in the asymmetric localization of ASH1 mRNANuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress
2054YFR002WNIC96Linker nucleoporin component of the nuclear pore complex (NPC)Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
2055YFR003CYPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7pRegulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
2056YFR004WRPN11Metalloprotease subunit of 19S regulatory particleMetalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
2057YFR005CSAD1Conserved zinc-finger domain protein involved in pre-mRNA splicingConserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle
2058YFR006WYFR006WPutative X-Pro aminopeptidasePutative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
2059YFR007WYFH7Putative kinase with similarity to the PRK/URK/PANK kinase subfamilyPutative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase
2060YFR008WFAR7Protein involved in recovery from pheromone-induced cell cycle arrestProtein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
2061YFR009WGCN20Positive regulator of the Gcn2p kinase activityPositive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
2062YFR009W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YFR009W; identified by expression profiling and mass spectrometry
2063YFR010WUBP6Ubiquitin-specific proteaseUbiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance
2064YFR010W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YFR011C; identified by expression profiling and mass spectrometry
2065YFR011CMIC19Component of the MICOS complexComponent of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane
2066YFR012WDCV1Protein of unknown functionProtein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication
2067YFR012W-AYFR012W-APutative protein of unknown functionPutative protein of unknown function; identified by homology
2068YFR013WIOC3Subunit of the Isw1a complexSubunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
2069YFR014CCMK1Calmodulin-dependent protein kinaseCalmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
2070YFR015CGSY1Glycogen synthaseGlycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
2071YFR016CYFR016CProtein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene
2072YFR017CIGD1Cytoplasmic protein that inhibits Gdb1p glycogen debranching activityCytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
2073YFR018CYFR018CPutative protein of unknown functionPutative protein of unknown function
2074YFR019WFAB11-phosphatidylinositol-3-phosphate 5-kinase1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
2075YFR020WCSS2Protein of unknown function, secreted when constitutively expressedProtein of unknown function; mRNA identified as translated by ribosome profiling data
2076YFR021WATG18Phosphoinositide binding proteinPhosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood
2077YFR022WROG3Alpha-arrestin involved in ubiquitin-dependent endocytosisAlpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; ROG3 has a paralog, ROD1, that arose from the whole genome duplication
2078YFR023WPES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutationPoly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
2079YFR024C-ALSB3Protein containing a C-terminal SH3 domainProtein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication
2080YFR025CHIS2HistidinolphosphataseHistidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
2081YFR026CULI1Protein of unknown functionPutative protein of unknown function; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR)
2082YFR027WECO1AcetyltransferaseAcetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress
2083YFR028CCDC14Protein phosphatase required for mitotic exitProtein phosphatase required for mitotic exit; required for rDNA segregation; located in nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis
2084YFR029WPTR3Component of the SPS plasma membrane amino acid sensor systemComponent of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
2085YFR030WMET10Subunit alpha of assimilatory sulfite reductaseSubunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide
2086YFR031CSMC2Subunit of the condensin complexSubunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus
2087YFR031C-ARPL2ARibosomal 60S subunit protein L2ARibosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
2088YFR032CRRT5Putative protein of unknown functionPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
2089YFR032C-ARPL29Ribosomal 60S subunit protein L29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
2090YFR032C-BMIN10Putative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2091YFR033CQCR6Subunit 6 of the ubiquinol cytochrome-c reductase complexSubunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
2092YFR034CPHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-familyBasic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability
2093YFR034W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YFR035C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
2094YFR035CYFR035CPutative protein of unknown functionPutative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein
2095YFR036WCDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia
2096YFR036W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF RSC8/YFR037C
2097YFR037CRSC8Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
2098YFR038WIRC5Putative ATPase containing the DEAD/H helicase-related sequence motifPutative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci
2099YFR039COSW7Protein involved in outer spore wall assemblyProtein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; deletion of both OSW7 and SHE10 results in reduced dityrosine fluorescence from the spore wall relative to other mutants
2100YFR040WSAP155Protein required for function of the Sit4p protein phosphataseProtein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication
2101YFR041CERJ5Type I membrane protein with a J domainType I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response
2102YFR042WKEG1Integral membrane protein of the ERIntegral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
2103YFR043CIRC6Clathrin coat accessory factorPutative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
2104YFR044CDUG1Cys-Gly metallo-di-peptidaseCys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
2105YFR045WYFR045WPutative mitochondrial transport proteinPutative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
2106YFR046CCNN1Kinetochore proteinKinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
2107YFR047CBNA6Quinolinate phosphoribosyl transferaseQuinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
2108YFR048WRMD8Cytosolic protein required for sporulationCytosolic protein required for sporulation
2109YFR049WYMR31Subunit of the mitochondrial alpha-ketoglutarate dehydrogenaseMitochondrial ribosomal protein of the small subunit; has similarity to human mitochondrial ribosomal protein MRP-S36
2110YFR050CPRE4Beta 7 subunit of the 20S proteasomeBeta 7 subunit of the 20S proteasome
2111YFR051CRET2Delta subunit of the coatomer complex (COPI)Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
2112YFR052C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF HXK1/YFR053C
2113YFR052WRPN12Subunit of the 19S regulatory particle of the 26S proteasome lidSubunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
2114YFR053CHXK1Hexokinase isoenzyme 1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
2115YFR054CYFR054CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2116YFR055WIRC7Beta-lyase involved in the production of thiolsBeta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner
2117YFR056CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W
2118YFR057WYFR057WPutative protein of unknown functionPutative protein of unknown function
2119YGL001CERG26C-3 sterol dehydrogenaseC-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
2120YGL002WERP6Member of the p24 family involved in ER to Golgi transportMember of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
2121YGL003CCDH1Activator of anaphase-promoting complex/cyclosome (APC/C)Activator of anaphase-promoting complex/cyclosome (APC/C); cell-cycle regulated; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
2122YGL004CRPN14Evolutionarily conserved 19S regulatory particle assembly-chaperoneAssembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
2123YGL005CCOG7Component of the conserved oligomeric Golgi complexComponent of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
2124YGL006WPMC1Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ionsVacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a
2125YGL006W-AYGL006W-APutative protein of unknown functionPutative protein of unknown function; identified by SAGE
2126YGL007C-AYGL007C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding
2127YGL007WBRP1Putative protein of unknown functionDubious ORF located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1
2128YGL008CPMA1Plasma membrane P2-type H+-ATPasePlasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
2129YGL009CLEU1Isopropylmalate isomeraseIsopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway
2130YGL010WMPO1Protein involved in metabolism of phytosphingosineProtein involved in metabolism of phytosphingosine; not an essential gene
2131YGL011CSCL1Alpha 1 subunit of the 20S proteasomeAlpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
2132YGL012WERG4C-24(28) sterol reductaseC-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
2133YGL013CPDR1Transcription factor that regulates the pleiotropic drug responseTranscription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
2134YGL014C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry
2135YGL014WPUF4Member of the PUF protein familyMember of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
2136YGL015CYGL015CPutative protein of unknown functionPutative protein of unknown function; null mutants accumulate cargo in the Golgi
2137YGL016WKAP122Karyopherin betaKaryopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance
2138YGL017WATE1Arginyl-tRNA-protein transferaseArginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
2139YGL018CJAC1Specialized J-protein that functions in Fe-S cluster biogenesisSpecialized J-protein that functions in Fe-S cluster biogenesis; functions with Hsp70 in Fe-S cluster biogenesis in mitochondria; involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
2140YGL019WCKB1Beta regulatory subunit of casein kinase 2 (CK2)Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
2141YGL020CGET1Subunit of the GET complexSubunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
2142YGL021WALK1Protein kinaseProtein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins
2143YGL022WSTT3Subunit of the oligosaccharyltransferase complex of the ER lumenSubunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis
2144YGL023CPIB2Protein of unknown functionProtein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
2145YGL024WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C
2146YGL025CPGD1Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
2147YGL026CTRP5Tryptophan synthaseTryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
2148YGL027CCWH41Processing alpha glucosidase IProcessing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
2149YGL028CSCW11Cell wall protein with similarity to glucanasesCell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
2150YGL029WCGR1Protein involved in nucleolar integrity and processing of pre-rRNAProtein involved in nucleolar integrity and processing of pre-rRNA; has a role in processing rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; relocalizes from nucleus to nucleolus upon DNA replication stress
2151YGL030WRPL30Ribosomal 60S subunit protein L30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
2152YGL031CRPL24ARibosomal 60S subunit protein L24ARibosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
2153YGL032CAGA2Adhesion subunit of a-agglutinin of a-cellsAdhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
2154YGL033WHOP2Meiosis-specific protein that localizes to chromosomesMeiosis-specific protein that localizes to chromosomes;preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair; contains a second intron at the 3' end; heterodimer of Hop2p-Mnd1p stimulates the activity of Dmc1p
2155YGL034CYGL034CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2156YGL035CMIG1Transcription factor involved in glucose repressionTranscription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
2157YGL036WYGL036WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene
2158YGL037CPNC1Nicotinamidase that converts nicotinamide to nicotinic acidNicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
2159YGL038COCH1Mannosyltransferase of the cis-Golgi apparatusMannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
2160YGL039WYGL039WAldehyde reductaseOxidoreductase shown to reduce carbonyl compounds to chiral alcohols
2161YGL040CHEM2Aminolevulinate dehydrataseAminolevulinate dehydratase; a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
2162YGL041CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2163YGL041C-BYGL041C-BPutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
2164YGL041W-ADPC13Putative protein of unknown functionPutative protein of unknown function; conserved in fungi; identified by expression profiling and mass spectrometry
2165YGL042CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
2166YGL043WDST1General transcription elongation factor TFIISGeneral transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
2167YGL044CRNA15Component of the cleavage and polyadenylation factor I (CF I)Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability
2168YGL045WRIM8Protein involved in proteolytic activation of Rim101pProtein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
2169YGL047WALG13Catalytic component of UDP-GlcNAc transferaseCatalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
2170YGL048CRPT6ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
2171YGL049CTIF4632Translation initiation factor eIF4GTranslation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
2172YGL050WTYW3tRNA methyltransferase required for synthesis of wybutosinetRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions
2173YGL051WMST27Putative integral membrane protein, involved in vesicle formationPutative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles; MST27 has a paralog, MST28, that arose from a segmental duplication
2174YGL052WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MST27
2175YGL053WPRM8Pheromone-regulated proteinPheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication
2176YGL054CERV14COPII-coated vesicle proteinCOPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
2177YGL055WOLE1Delta(9) fatty acid desaturaseDelta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
2178YGL056CSDS23Protein involved in cell separation during buddingProtein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication
2179YGL057CGEP7Protein of unknown functionProtein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2180YGL058WRAD6Ubiquitin-conjugating enzyme (E2)Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, as well as endoplasmic reticulum-associated protein degradation (ERAD) with Ubr1p in the absence of canonical ER membrane ligases
2181YGL059WPKP2Mitochondrial protein kinaseMitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
2182YGL060WYBP2Central kinetochore associated proteinCentral kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
2183YGL061CDUO1Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
2184YGL062WPYC1Pyruvate carboxylase isoformPyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
2185YGL063C-ADubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2186YGL063WPUS2Mitochondrial tRNA:pseudouridine synthaseMitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication
2187YGL064CMRH4Mitochondrial ATP-dependent RNA helicase of the DEAD-box familyMitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes
2188YGL065CALG2Mannosyltransferase in the N-linked glycosylation pathwayMannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
2189YGL066WSGF73Subunit of DUBm module of SAGA and SLIKSAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; relocalizes to the cytosol in response to hypoxia; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
2190YGL067WNPY1NADH diphosphatase (pyrophosphatase)NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
2191YGL068WMNP1Mitochondrial ribosomal protein of the large subunitProtein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
2192YGL069CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
2193YGL070CRPB9RNA polymerase II subunit B12.6RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
2194YGL071WAFT1Transcription factor involved in iron utilization and homeostasisTranscription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
2195YGL072CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
2196YGL073WHSF1Trimeric heat shock transcription factorTrimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses, including hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell at the ribosome through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated
2197YGL074CDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
2198YGL075CMPS2Essential membrane protein localized at nuclear envelope and SPBsEssential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication
2199YGL076CRPL7ARibosomal 60S subunit protein L7ARibosomal 60S subunit protein L7A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication
2200YGL077CHNM1Plasma membrane transporter for choline, ethanolamine, and carnitinePlasma membrane transporter for choline, ethanolamine, and carnitine; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
2201YGL078CDBP3RNA-Dependent ATPase, member of DExD/H-box familyRNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
2202YGL079WKXD1Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
2203YGL080WMPC1Highly conserved subunit of mitochondrial pyruvate carrier (MPC)Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Mpc1p and either Mpc2p or Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia
2204YGL081WYGL081WPutative protein of unknown functionPutative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
2205YGL082WYGL082WPutative protein of unknown functionPutative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication
2206YGL083WSCY1Putative kinasePutative kinase; suppressor of GTPase mutant, similar to bovine rhodopsin kinase
2207YGL084CGUP1Plasma membrane protein involved in remodeling GPI anchorsPlasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; GUP1 has a paralog, GUP2, that arose from the whole genome duplication
2208YGL085WLCL3Putative protein of unknown functionPutative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
2209YGL086WMAD1Coiled-coil protein involved in spindle-assembly checkpointCoiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
2210YGL087CMMS2Ubiquitin-conjugating enzyme variantUbiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
2211YGL088WYGL088WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
2212YGL089CMF(ALPHA)2Mating pheromone alpha-factor, made by alpha cellsMating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed; MF(ALPHA)2 has a paralog, MF(ALPHA)1, that arose from the whole genome duplication
2213YGL090WLIF1Component of the DNA ligase IV complexComponent of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
2214YGL091CNBP35Essential cytoplasmic iron-sulfur cluster binding proteinEssential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
2215YGL092WNUP145Essential protein with distinct roles in two nuclear pore subcomplexesEssential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
2216YGL093WSPC105Subunit of a kinetochore-microtubule binding complexSubunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation
2217YGL094CPAN2Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complexCatalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; complex acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
2218YGL095CVPS45Protein of the Sec1p/Munc-18 familyProtein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
2219YGL096WTOS8Homeodomain-containing protein and putative transcription factorHomeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
2220YGL097WSRM1Nucleotide exchange factor for Gsp1pNucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
2221YGL098WUSE1Essential SNARE protein localized to the EREssential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
2222YGL099WLSG1Putative GTPase involved in 60S ribosomal subunit biogenesisPutative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
2223YGL100WSEH1Subunit of the Nup84 nuclear pore and SEACAT subcomplexesComponent of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
2224YGL101WYGL101WProtein of unknown functionProtein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication
2225YGL102CDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
2226YGL103WRPL28Ribosomal 60S subunit protein L28Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance
2227YGL104CVPS73Mitochondrial proteinMitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family; VPS73 has a paralog, YBR241C, that arose from the whole genome duplication
2228YGL105WARC1Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetasesProtein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p
2229YGL106WMLC1Essential light chain for Myo1pEssential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition
2230YGL107CRMD9Mitochondrial protein required for respiratory growthMitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes; RMD9 has a paralog, YBR238C, that arose from the whole genome duplication
2231YGL108CYGL108CProtein of unknown function, predicted to be palmitoylatedProtein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
2232YGL109WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
2233YGL110CCUE3Protein of unknown functionProtein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
2234YGL111WNSA1Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
2235YGL112CTAF6Subunit (60 kDa) of TFIID and SAGA complexesSubunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia
2236YGL113WSLD3Protein involved in the initiation of DNA replicationProtein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
2237YGL114WYGL114WPutative protein of unknown functionPutative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
2238YGL115WSNF4Activating gamma subunit of the AMP-activated Snf1p kinase complexActivating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
2239YGL116WCDC20Activator of anaphase-promoting complex/cyclosome (APC/C)Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
2240YGL117WYGL117WPutative protein of unknown functionPutative protein of unknown function
2241YGL118CYGL118CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2242YGL119WCOQ8ATPase required for ubiquinone biosynthesis and respiratory growthProtein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
2243YGL120CPRP43RNA helicase in the DEAH-box familyRNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
2244YGL121CGPG1Proposed gamma subunit of the heterotrimeric G proteinProposed gamma subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing
2245YGL122CNAB2Nuclear polyadenylated RNA-binding proteinNuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
2246YGL123C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene RPS2/YGL123W
2247YGL123WRPS2Protein component of the small (40S) subunitProtein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
2248YGL124CMON1Subunit of a heterodimeric guanine nucleotide exchange factor (GEF)Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate
2249YGL125WMET13Major isozyme of methylenetetrahydrofolate reductaseMajor isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
2250YGL126WSCS3Protein required for inositol prototrophyProtein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
2251YGL127CSOH1Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
2252YGL128CCWC23Component of a complex containing Cef1pComponent of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
2253YGL129CRSM23Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
2254YGL130WCEG1Guanylyltransferase involved in mRNA 5' cappingGuanylyltransferase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5?-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide
2255YGL131CSNT2Subunit of Snt2C complex, RING finger ubiquitin ligase (E3)Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitylation, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
2256YGL132WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C
2257YGL133WITC1Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complexSubunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication
2258YGL134WPCL10Pho85p cyclinPho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication
2259YGL135WRPL1BRibosomal 60S subunit protein L1BRibosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
2260YGL136CMRM2Mitochondrial 2' O-ribose methyltransferaseMitochondrial 2' O-ribose methyltransferase; required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
2261YGL137WSEC27Essential beta'-coat protein of the COPI coatomerEssential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
2262YGL138CYGL138CPutative protein of unknown functionPutative protein of unknown function; has no significant sequence similarity to any known protein
2263YGL139WFLC3Putative FAD transporter, similar to Flc1p and Flc2pPutative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that arose from the whole genome duplication
2264YGL140CYGL140CPutative protein of unknown functionPutative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains
2265YGL141WHUL5Multiubiquitin chain assembly factor (E4)Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD
2266YGL142CGPI10Integral membrane protein involved in GPI anchor synthesisIntegral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog
2267YGL143CMRF1Mitochondrial translation release factorMitochondrial translation release factor; involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
2268YGL144CROG1Lipase with specificity for monoacylglycerolProtein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication
2269YGL145WTIP20Peripheral membrane protein required for COPI vesicle fusion to the ERPeripheral membrane protein required for COPI vesicle fusion to the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
2270YGL146CRRT6Putative protein of unknown functionPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
2271YGL147CRPL9ARibosomal 60S subunit protein L9ARibosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication
2272YGL148WARO2Bifunctional chorismate synthase and flavin reductaseBifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
2273YGL149WYGL149WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C
2274YGL150CINO80ATPase and nucleosome spacing factorATPase and nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; promotes nucleosome shifts in the 3 prime direction; has a role in modulating stress gene transcription
2275YGL151WNUT1Component of the RNA polymerase II mediator complexComponent of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
2276YGL152CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
2277YGL153WPEX14Central component of the peroxisomal importomer complexCentral component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
2278YGL154CLYS5Phosphopantetheinyl transferase involved in lysine biosynthesisPhosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
2279YGL155WCDC43Beta subunit of geranylgeranyltransferase type IBeta subunit of geranylgeranyltransferase type I; catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
2280YGL156WAMS1Vacuolar alpha mannosidaseVacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
2281YGL157WARI1NADPH-dependent aldehyde reductaseNADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
2282YGL158WRCK1Protein kinase involved in oxidative stress responseProtein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
2283YGL159WYGL159WPutative protein of unknown functionPutative protein of unknown function; deletion mutant has no detectable phenotype
2284YGL160WAIM14NADPH oxidase localized to the perinuclear ERNADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
2285YGL161CYIP5Protein that interacts with Rab GTPasesProtein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
2286YGL162WSUT1Zn(II)2Cys6 family transcription factorTranscription factor of the Zn(II)2Cys6 family; positively regulates genes involved in sterol uptake under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during non-starvation conditions; positively regulates mating with SUT2 by repressing expression of genes which act as mating inhibitors; relocalizes from nucleus to cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication
2287YGL163CRAD54DNA-dependent ATPase that stimulates strand exchangeDNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
2288YGL164CYRB30RanGTP-binding proteinRanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
2289YGL165CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
2290YGL166WCUP2Copper-binding transcription factorCopper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
2291YGL167CPMR1High affinity Ca2+/Mn2+ P-type ATPaseHigh affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen appears to be required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
2292YGL168WHUR1Protein of unknown functionProtein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
2293YGL169WSUA5Protein involved in threonylcarbamoyl adenosine biosynthesisProtein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length
2294YGL170CSPO74Component of the meiotic outer plaque of the spindle pole bodyComponent of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
2295YGL171WROK1RNA-dependent ATPaseRNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
2296YGL172WNUP49FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
2297YGL173CXRN1Evolutionarily-conserved 5'-3' exonucleaseEvolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; also enters the nucleus and positively regulates transcription initiation and elongation; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p
2298YGL174WBUD13Subunit of the RES complexSubunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
2299YGL175CSAE2Endonuclease required for telomere elongationEndonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently
2300YGL176CYGL176CPutative protein of unknown functionPutative protein of unknown function; deletion mutant is viable and has no detectable phenotype
2301YGL177WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2302YGL178WMPT5mRNA-binding protein of the PUF familymRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
2303YGL179CTOS3Protein kinaseProtein kinase; related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication
2304YGL180WATG1Protein serine/threonine kinaseProtein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
2305YGL181WGTS1Protein involved in Arf3p regulation and in transcription regulationProtein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
2306YGL182CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MND1/YGL183C
2307YGL183CMND1Protein required for recombination and meiotic nuclear divisionProtein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks
2308YGL184CSTR3Peroxisomal cystathionine beta-lyasePeroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation
2309YGL185CYGL185CPutative protein with sequence similar to hydroxyacid dehydrogenasesPutative protein with sequence similar to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
2310YGL186CTPN1Plasma membrane pyridoxine (vitamin B6) transporterPlasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
2311YGL187CCOX4Subunit IV of cytochrome c oxidaseSubunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation
2312YGL188CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2313YGL188C-AYGL188C-APutative protein of unknown functionPutative protein of unknown function
2314YGL189CRPS26AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26A has a paralog, RPS26B, that arose from the whole genome duplication
2315YGL190CCDC55Regulatory subunit B of protein phosphatase 2A (PP2A)Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55
2316YGL191WCOX13Subunit VIa of cytochrome c oxidaseSubunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
2317YGL192WIME4mRNA N6-adenosine methyltransferase required for entry into meiosismRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70)
2318YGL193CYGL193CHaploid-specific gene repressed by a1-alpha2Haploid-specific gene repressed by a1-alpha2; turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold
2319YGL194CHOS2Histone deacetylase and subunit of Set3 and Rpd3L complexesHistone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
2320YGL194C-AYGL194C-APutative protein of unknown functionPutative protein of unknown function; identified based on comparisons of the genome sequences of six Saccharomyces species
2321YGL195WGCN1Positive regulator of the Gcn2p kinase activityPositive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA
2322YGL196WDSD1D-serine dehydratase (aka D-serine ammonia-lyase)D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
2323YGL197WMDS3Putative component of the TOR regulatory pathwayPutative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication
2324YGL198WYIP4Protein that interacts with Rab GTPasesProtein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
2325YGL199CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
2326YGL200CEMP24Component of the p24 complexComponent of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
2327YGL201CMCM6Protein involved in DNA replicationProtein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
2328YGL202WARO8Aromatic aminotransferase IAromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis
2329YGL203CKEX1Cell death protease essential for hypochlorite-induced apoptosisCell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
2330YGL204CYGL204CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data
2331YGL205WPOX1Fatty-acyl coenzyme A oxidaseFatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
2332YGL206CCHC1Clathrin heavy chainClathrin heavy chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion; the light chain (CLC1) is thought to regulate function
2333YGL207WSPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p)Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; SPT16 specifically required for diauxic shift-induced H2B deposition onto rDNA genes; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
2334YGL208WSIP2One of three beta subunits of the Snf1 kinase complexOne of three beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication
2335YGL209WMIG2Zinc finger transcriptional repressorZinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose
2336YGL210WYPT32Rab family GTPase involved in the exocytic pathwayRab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
2337YGL211WNCS6Protein required for uridine thiolation of Gln, Lys, and Glu tRNAsProtein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
2338YGL212WVAM7Vacuolar SNARE proteinVacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
2339YGL213CSKI8Ski complex component and WD-repeat proteinSki complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype
2340YGL214WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C
2341YGL215WCLG1Cyclin-like protein that interacts with Pho85pCyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
2342YGL216WKIP3Kinesin-related antiparallel sliding motor proteinKinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone
2343YGL217CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
2344YGL218WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
2345YGL219CMDM34Mitochondrial component of the ERMES complexMitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
2346YGL220WBOL2Cytosolic protein involved in repression of iron regulon transcriptionProtein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
2347YGL221CNIF3Protein of unknown functionProtein of unknown function; similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2348YGL222CEDC1RNA-binding protein that activates mRNA decapping directlyRNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication
2349YGL223CCOG1Essential component of the conserved oligomeric Golgi complexEssential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
2350YGL224CSDT1Pyrimidine nucleotidasePyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication
2351YGL225WVRG4Golgi GDP-mannose transporterGolgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication
2352YGL226C-AOST5Zeta subunit of the oligosaccharyltransferase complex of the ER lumenZeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
2353YGL226WMTC3Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
2354YGL227WVID30Central component of GID Complex, involved in FBPase degradationCentral component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
2355YGL228WSHE10Protein involved in outer spore wall assemblyProtein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication and deletion of both SHE10 and OWS7 results in reduced dityrosine fluorescence from the spore wall relative to other mutants
2356YGL229CSAP4Protein required for function of the Sit4p protein phosphataseProtein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication
2357YGL230CYGL230CPutative protein of unknown functionPutative protein of unknown function; non-essential gene
2358YGL231CEMC4Member of conserved ER transmembrane complexMember of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85
2359YGL232WTAN1Putative tRNA acetyltransferasePutative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
2360YGL233WSEC15Essential 113 kDa subunit of the exocyst complexEssential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
2361YGL234WADE5,7Enzyme of the 'de novo' purine nucleotide biosynthetic pathwayEnzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
2362YGL235WYGL235WPutative protein of unknown functionPutative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
2363YGL236CMTO1Mitochondrial proteinMitochondrial protein; forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
2364YGL237CHAP2Subunit of the Hap2p/3p/4p/5p CCAAT-binding complexSubunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
2365YGL238WCSE1Nuclear envelope protein that acts as a recycling factorNuclear envelope protein that mediates the nuclear export of Srp1p; Srp1p (importin alpha) is a homolog of metazoan CAS protein; required for accurate chromosome segregation
2366YGL239CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene CSE1
2367YGL240WDOC1Processivity factorProcessivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain
2368YGL241WKAP114Karyopherin, responsible for nuclear import of specific proteinsKaryopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
2369YGL242CYGL242CProtein of unknown functionProtein of unknown function; N-terminally propionylated in vivo; deletion mutant is viable
2370YGL243WTAD1tRNA-specific adenosine deaminasetRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala
2371YGL244WRTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complexSubunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
2372YGL245WGUS1Glutamyl-tRNA synthetase (GluRS)Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
2373YGL246CRAI1Nuclear protein with decapping endonuclease activityNuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z
2374YGL247WBRR6Essential nuclear envelope integral membrane proteinEssential nuclear envelope integral membrane protein; required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
2375YGL248WPDE1Low-affinity cyclic AMP phosphodiesteraseLow-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
2376YGL249WZIP2Meiosis-specific proteinMeiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
2377YGL250WRMR1Protein required for meiotic recombination and gene conversionProtein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
2378YGL251CHFM1Meiosis specific DNA helicaseMeiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
2379YGL252CRTG2Sensor of mitochondrial dysfunctionSensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
2380YGL253WHXK2Hexokinase isoenzyme 2Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication
2381YGL254WFZF1Transcription factor involved in sulfite metabolismTranscription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
2382YGL255WZRT1High-affinity zinc transporter of the plasma membraneHigh-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
2383YGL256WADH4Alcohol dehydrogenase isoenzyme type IVAlcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
2384YGL257CMNT2MannosyltransferaseMannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans
2385YGL258WVEL1Protein of unknown functionProtein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication
2386YGL258W-AYGL258W-APutative protein of unknown functionPutative protein of unknown function
2387YGL259WYPS5Protein with similarity to GPI-anchored aspartic proteasesProtein with similarity to GPI-anchored aspartic proteases; such proteases are Yap1p and Yap3p
2388YGL260WYGL260WPutative protein of unknown functionPutative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
2389YGL261CPAU11Putative protein of unknown functionPutative protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
2390YGL262WYGL262WPutative protein of unknown functionPutative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
2391YGL263WCOS12Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
2392YGR001CEFM5S-adenosylmethionine-dependent lysine methyltransferasePutative protein of unknown function; similar to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae; expresses a circular RNA
2393YGR002CSWC4Component of the Swr1p complex that incorporates Htz1p into chromatinComponent of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
2394YGR003WCUL3Ubiquitin-protein ligaseUbiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
2395YGR004WPEX31Peroxisomal integral membrane proteinPeroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication
2396YGR005CTFG2TFIIF (Transcription Factor II) middle subunitTFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
2397YGR006WPRP18Splicing factor and component of snRNP U5Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p
2398YGR007WECT1Ethanolamine-phosphate cytidylyltransferaseEthanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases
2399YGR008CSTF2Protein involved in resistance to desiccation stressProtein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication
2400YGR009CSEC9t-SNARE protein required for secretory vesicle-plasma membrane fusiont-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functionally redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog
2401YGR010WNMA2Nicotinic acid mononucleotide adenylyltransferaseNicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication
2402YGR011WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2403YGR012WMCY1Putative cysteine synthasePutative cysteine synthase; localized to the mitochondrial outer membrane
2404YGR013WSNU71Component of U1 snRNP required for mRNA splicing via spliceosomeComponent of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
2405YGR014WMSB2Mucin family member involved in various signaling pathwaysMucin family member involved in various signaling pathways; functions as osmosensor in Sho1p-mediated HOG pathway with Msb2p; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate
2406YGR015CEAT1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
2407YGR016WYGR016WPutative protein of unknown functionPutative protein of unknown function
2408YGR017WYGR017WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
2409YGR018CYGR018CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
2410YGR019WUGA1Gamma-aminobutyrate (GABA) transaminaseGamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
2411YGR020CVMA7Subunit F of the V1 peripheral membrane domain of V-ATPaseSubunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
2412YGR021WDPC29Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2413YGR022CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
2414YGR023WMTL1Putative plasma membrane sensorPutative plasma membrane sensor; involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication
2415YGR024CTHG1tRNAHis guanylyltransferasetRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
2416YGR025WYGR025WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2417YGR026WYGR026WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
2418YGR027CRPS25AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication
2419YGR027W-AYGR027W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2420YGR027W-BYGR027W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2421YGR028WMSP1Highly-conserved N-terminally anchored AAA-ATPaseHighly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
2422YGR029WERV1Flavin-linked sulfhydryl oxidase of the mitochondrial IMSFlavin-linked sulfhydryl oxidase of the mitochondrial IMS; N-terminus is an intrinsically disordered domain that in the cytosol helps target Erv1p to mitochondria, and in the intermembrane space oxidizes Mia40p as part of a disulfide relay system that promotes intermembrane space retention of imported proteins; functional ortholog of human GFER (ALR)
2423YGR030CPOP6Subunit of RNase MRP, nuclear RNase P and telomeraseSubunit of both RNase MRP and nuclear RNase P; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
2424YGR031C-ANAG1Protein involved in yeast cell wall biogenesisProtein involved in yeast cell wall biogenesis; localizes to the cell periphery; production of Nag1p is dependent upon the presence of Slt2p and Rlm1p; gene is nested within and antisense to IMO32
2425YGR031WIMO32Conserved mitochondrial protein of unknown functionConserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
2426YGR032WGSC2Catalytic subunit of 1,3-beta-glucan synthaseCatalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication
2427YGR033CTIM21Nonessential component of the TIM23 complexNonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity
2428YGR034WRPL26BRibosomal 60S subunit protein L26BRibosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
2429YGR035CYGR035CPutative protein of unknown function, potential Cdc28p substratePutative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication
2430YGR035W-AYGR035W-APutative protein of unknown functionPutative protein of unknown function
2431YGR036CCAX4Dolichyl pyrophosphate (Dol-P-P) phosphataseDolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminally oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
2432YGR037CACB1Acyl-CoA-binding proteinAcyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
2433YGR038C-AYGR038C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2434YGR038C-BYGR038C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2435YGR038WORM1Protein that mediates sphingolipid homeostasisProtein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication
2436YGR039WYGR039WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722
2437YGR040WKSS1Mitogen-activated protein kinase (MAPK)Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
2438YGR041WBUD9Protein involved in bud-site selectionProtein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication
2439YGR042WMTE1Protein of unknown functionProtein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
2440YGR043CNQM1Transaldolase of unknown functionTransaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication
2441YGR044CRME1Zinc finger protein involved in control of meiosisZinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
2442YGR045CYGR045CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2443YGR046WTAM41Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase)Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase); required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
2444YGR047CTFC4Subunit of RNA polymerase III transcription initiation factor complexSubunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
2445YGR048WUFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregaseSubstrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
2446YGR049WSCM4Mitochondrial outer membrane protein of unknown functionMitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication
2447YGR050CYGR050CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data
2448YGR051CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene
2449YGR052WFMP48Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
2450YGR053CMCO32Putative protein of unknown functionPutative protein of unknown function
2451YGR054WYGR054WEukaryotic initiation factor eIF2AEukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
2452YGR055WMUP1High affinity methionine permeaseHigh affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
2453YGR056WRSC1Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication
2454YGR057CLST7Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2pProtein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
2455YGR058WPEF1Penta-EF-hand proteinPenta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
2456YGR059WSPR3Sporulation-specific homolog of the CDC3/10/11/12 family of genesSporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
2457YGR060WERG25C-4 methyl sterol oxidaseC-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
2458YGR061CADE6Formylglycinamidine-ribonucleotide (FGAM)-synthetaseFormylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
2459YGR062CCOX18Protein required for membrane insertion of C-terminus of Cox2pProtein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC
2460YGR063CSPT4Spt4p/5p (DSIF) transcription elongation factor complex subunitComponent of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
2461YGR064WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
2462YGR065CVHT1High-affinity plasma membrane H+-biotin (vitamin H) symporterHigh-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
2463YGR066CYGR066CPutative protein of unknown functionPutative protein of unknown function
2464YGR067CYGR067CPutative protein of unknown functionPutative protein of unknown function; contains a zinc finger motif similar to that of Adr1p
2465YGR068CART5Protein proposed to regulate endocytosis of plasma membrane proteinsProtein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane
2466YGR068W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with the uncharacterized ORF YGR068C
2467YGR069WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2468YGR070WROM1Guanine nucleotide exchange factor (GEF) for Rho1pGDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication
2469YGR071CENV11Protein proposed to be involved in vacuolar functionsProtein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
2470YGR072WUPF3Component of the nonsense-mediated mRNA decay (NMD) pathwayComponent of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
2471YGR073CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
2472YGR074WSMD1Core Sm protein Sm D1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
2473YGR075CPRP38Unique component of the U4/U6.U5 tri-snRNP particleUnique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly
2474YGR076CMRPL25Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
2475YGR077CPEX8Intraperoxisomal organizer of the peroxisomal import machineryIntraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
2476YGR078CPAC10Part of the heteromeric co-chaperone GimC/prefoldin complexPart of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding
2477YGR079WYGR079WPutative protein of unknown functionPutative protein of unknown function; YGR079W is not an essential gene
2478YGR080WTWF1TwinfilinTwinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly
2479YGR081CSLX9Protein required for pre-rRNA processingProtein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
2480YGR082WTOM20Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
2481YGR083CGCD2Delta subunit of the translation initiation factor eIF2BDelta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
2482YGR084CMRP13Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
2483YGR085CRPL11BRibosomal 60S subunit protein L11BRibosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
2484YGR086CPIL1Eisosome core componentEisosome core component; eisosomes are large immobile cell cortex structures associated with endocytosis; detected in phosphorylated state in mitochondria; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; member of BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
2485YGR087CPDC6Minor isoform of pyruvate decarboxylaseMinor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
2486YGR088WCTT1Cytosolic catalase TCytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide
2487YGR089WNNF2Protein that exhibits physical and genetic interactions with Rpb8pProtein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
2488YGR090WUTP22Component of the small-subunit processomeComponent of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
2489YGR091WPRP31Splicing factorSplicing factor; component of the U4/U6-U5 snRNP complex
2490YGR092WDBF2Ser/Thr kinase involved in transcription and stress responseSer/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
2491YGR093WDRN1Splicing factor that modulates turnover of branched RNAs by Dbr1pSplicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia
2492YGR094WVAS1Mitochondrial and cytoplasmic valyl-tRNA synthetaseMitochondrial and cytoplasmic valyl-tRNA synthetase
2493YGR095CRRP46Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
2494YGR096WTPC1Mitochondrial membrane transporterMitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
2495YGR097WASK10Regulator of the Fps1p glycerol channelComponent of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
2496YGR098CESP1Separase, a caspase-like cysteine proteaseSeparase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
2497YGR099WTEL2Subunit of the ASTRA complex, involved in chromatin remodelingSubunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
2498YGR100WMDR1Cytoplasmic GTPase-activating proteinCytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
2499YGR101WPCP1Mitochondrial serine proteaseMitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
2500YGR102CGTF1Subunit of the trimeric GatFAB AmidoTransferase(AdT) complexSubunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
2501YGR103WNOP7Component of several different pre-ribosomal particlesComponent of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
2502YGR104CSRB5Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
2503YGR105WVMA21Integral membrane protein required for V-ATPase functionIntegral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
2504YGR106CVOA1ER protein that functions in assembly of the V0 sector of V-ATPaseER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval
2505YGR107WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2506YGR108WCLB1B-type cyclin involved in cell cycle progressionB-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication
2507YGR109CCLB6B-type cyclin involved in DNA replication during S phaseB-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
2508YGR109W-AYGR109W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2509YGR109W-BYGR109W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2510YGR110WCLD1Mitochondrial cardiolipin-specific phospholipaseMitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
2511YGR111WYGR111WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
2512YGR112WSHY1Mitochondrial inner membrane protein required for complex IV assemblyMitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase
2513YGR113WDAM1Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
2514YGR114CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
2515YGR115CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
2516YGR116WSPT6Nucleosome remodeling proteinNucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p
2517YGR117CYGR117CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
2518YGR118WRPS23ARibosomal protein 28 (rp28) of the small (40S) ribosomal subunitRibosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
2519YGR119CNUP57FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
2520YGR120CCOG2Essential component of the conserved oligomeric Golgi complexEssential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p
2521YGR121CMEP1Ammonium permeaseAmmonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation; MEP1 has a paralog, MEP3, that arose from the whole genome duplication
2522YGR121W-AYGR121W-APutative protein of unknown functionPutative protein of unknown function
2523YGR122C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; similar to YLR334C and YOL106W
2524YGR122WYGR122WProtein that may be involved in pH regulationProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
2525YGR123CPPT1Protein serine/threonine phosphataseProtein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
2526YGR124WASN2Asparagine synthetaseAsparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
2527YGR125WYGR125WPutative protein of unknown functionPutative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
2528YGR126WYGR126WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
2529YGR127WYGR127WPutative protein of unknown functionPutative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
2530YGR128CUTP8Nucleolar protein required for export of tRNAs from the nucleusNucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
2531YGR129WSYF2Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest
2532YGR130CYGR130CComponent of the eisosome with unknown functionComponent of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
2533YGR131WFHN1Protein of unknown functionProtein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication
2534YGR132CPHB1Subunit of the prohibitin complex (Phb1p-Phb2p)Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
2535YGR133WPEX4Peroxisomal ubiquitin conjugating enzymePeroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis
2536YGR134WCAF130Subunit of the CCR4-NOT transcriptional regulatory complexSubunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation
2537YGR135WPRE9Alpha 3 subunit of the 20S proteasomeAlpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
2538YGR136WLSB1Negative regulator of actin nucleation-promoting factor activityNegative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication
2539YGR137WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2540YGR138CTPO2Polyamine transporter of the major facilitator superfamilyPolyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
2541YGR139WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2542YGR140WCBF2Essential kinetochore proteinEssential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
2543YGR141WVPS62Vacuolar protein sorting (VPS) proteinVacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; VPS62 has a paralog, TDA6, that arose from the whole genome duplication
2544YGR142WBTN2v-SNARE binding proteinv-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; BTN2 has a paralog, CUR1, that arose from the whole genome duplication
2545YGR143WSKN1Protein involved in sphingolipid biosynthesisProtein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication
2546YGR144WTHI4Thiazole synthaseThiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
2547YGR145WENP2Component of the SSUComponent of the SSU; required for pre-18S rRNA processing, biogenesis of the small ribosomal subunit; interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD repeats, and has homology to Spb1p
2548YGR146CECL1Protein of unknown functionProtein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
2549YGR146C-AYGR146C-APutative protein of unknown functionPutative protein of unknown function
2550YGR147CNAT2Protein of unknown functionProtein of unknown function; has an apparent role in acetylation of N-terminal methionine residues
2551YGR148CRPL24BRibosomal 60S subunit protein L24BRibosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
2552YGR149WGPC1Glycerophosphocholine acyltransferase (GPCAT)Putative protein of unknown function; predicted to be an integal membrane protein
2553YGR150CCCM1Mitochondrial 15S rRNA-binding proteinMitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
2554YGR151CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
2555YGR152CRSR1GTP-binding protein of the Ras superfamilyGTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
2556YGR153WYGR153WPutative protein of unknown functionPutative protein of unknown function
2557YGR154CGTO1Omega-class glutathione transferaseOmega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
2558YGR155WCYS4Cystathionine beta-synthaseCystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
2559YGR156WPTI1Essential component of CPF (cleavage and polyadenylation factor)Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64
2560YGR157WCHO2Phosphatidylethanolamine methyltransferase (PEMT)Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
2561YGR158CMTR3Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
2562YGR159CNSR1Nucleolar protein that binds nuclear localization sequencesNucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis
2563YGR160WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2564YGR161CRTS3Putative component of the protein phosphatase type 2A complexPutative component of the protein phosphatase type 2A complex
2565YGR161C-CYGR161C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2566YGR161C-DYGR161C-DRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2567YGR161W-AYGR161W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2568YGR161W-BYGR161W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2569YGR161W-CYGR161W-CPutative protein of unknown functionPutative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
2570YGR162WTIF4631Translation initiation factor eIF4GTranslation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
2571YGR163WGTR2Subunit of a TORC1-stimulating GTPase and the EGO/GSE complexPutative GTP binding protein; negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
2572YGR164WYGR164WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2573YGR165WMRPS35Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
2574YGR166WTRS65Component of transport protein particle (TRAPP) complex IIComponent of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response
2575YGR167WCLC1Clathrin light chainClathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
2576YGR168CPEX35Putative protein of unknown functionPutative protein of unknown function; YGR168C is not an essential gene
2577YGR169CPUS6tRNA:pseudouridine synthasetRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
2578YGR169C-ALSO2Protein with a potential role in response to iron deprivationPutative protein of unknown function; LSO2 has a paralog, LSO1, that arose from the whole genome duplication
2579YGR170WPSD2Phosphatidylserine decarboxylase of the Golgi and vacuolar membranesPhosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventually give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected
2580YGR171CMSM1Mitochondrial methionyl-tRNA synthetase (MetRS)Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
2581YGR172CYIP1Integral membrane proteinIntegral membrane protein; required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
2582YGR173WRBG2Protein with a role in translationProtein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
2583YGR174CCBP4Mitochondrial protein required for assembly of cytochrome bc1 complexMitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
2584YGR174W-AYGR174W-APutative protein of unknown functionPutative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis
2585YGR175CERG1Squalene epoxidaseSqualene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
2586YGR176WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2587YGR177CATF2Alcohol acetyltransferaseAlcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
2588YGR178CPBP1Component of glucose deprivation induced stress granulesComponent of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
2589YGR179COKP1Outer kinetochore protein required for accurate chromosome segregationOuter kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7
2590YGR180CRNR4Ribonucleotide-diphosphate reductase (RNR) small subunitRibonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication
2591YGR181WTIM13Mitochondrial intermembrane space proteinMitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
2592YGR182CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W
2593YGR183CQCR9Subunit 9 of ubiquinol cytochrome-c reductase (Complex III)Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
2594YGR184CUBR1E3 ubiquitin ligase (N-recognin)E3 ubiquitin ligase (N-recognin); heterodimerizes with Rad6p to ubiquitinate substrates in the N-end rule pathway; role in endoplasmic reticulum-associated protein degradation (ERAD) in the absence of canonical ER membrane ligases or after stress; major role in targeting misfolded cytosolic proteins for degradation; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
2595YGR185CTYS1Cytoplasmic tyrosyl-tRNA synthetaseCytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
2596YGR186WTFG1TFIIF (Transcription Factor II) largest subunitTFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
2597YGR187CHGH1Nonessential protein of unknown functionNonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
2598YGR188CBUB1Protein kinase involved in the cell cycle checkpoint into anaphaseProtein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
2599YGR189CCRH1Chitin transglycosylaseChitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
2600YGR190CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
2601YGR191WHIP1High-affinity histidine permeaseHigh-affinity histidine permease; also involved in the transport of manganese ions
2602YGR192CTDH3Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria; binds AU-rich RNA; TDH3 has a paralog, TDH2, that arose from the whole genome duplication
2603YGR193CPDX1E3-binding protein of the mitochondrial pyruvate dehydrogenase complexE3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core
2604YGR194CXKS1XylulokinaseXylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
2605YGR195WSKI6Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
2606YGR196CFYV8Protein of unknown functionProtein of unknown function; required for survival upon exposure to K1 killer toxin
2607YGR197CSNG1Protein involved in resistance to nitrosoguanidine and 6-azauracilProtein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication
2608YGR198WYPP1Cargo-transport protein involved in endocytosisCargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4p; is required, along with Efr3p, for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches at the plasma membrane; positively regulates Stt4p; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
2609YGR199WPMT6Protein O-mannosyltransferaseProtein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
2610YGR200CELP2Subunit of Elongator complexSubunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
2611YGR201CYGR201CPutative protein of unknown functionPutative protein of unknown function
2612YGR202CPCT1Cholinephosphate cytidylyltransferaseCholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
2613YGR203WYCH1Phosphatase with sequence similarity to Cdc25pPhosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
2614YGR204C-AYGR204C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2615YGR204WADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthaseCytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
2616YGR205WTDA10ATP-binding protein of unknown functionATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
2617YGR206WMVB12ESCRT-I subunit required to stabilize ESCRT-I core complex oligomersESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
2618YGR207CCIR1Mitochondrial protein that interacts with frataxin (Yfh1p)Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
2619YGR208WSER2Phosphoserine phosphatase of the phosphoglycerate pathwayPhosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
2620YGR209CTRX2Cytoplasmic thioredoxin isoenzymeCytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication
2621YGR210CYGR210CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
2622YGR211WZPR1Essential protein with two zinc fingersEssential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
2623YGR212WSLI1N-acetyltransferaseN-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
2624YGR213CRTA1Protein involved in 7-aminocholesterol resistanceProtein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication
2625YGR214WRPS0ARibosomal 40S subunit protein S0ARibosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
2626YGR215WRSM27Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
2627YGR216CGPI1Membrane protein involved in the synthesis of GlcNAc-PIMembrane protein involved in the synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
2628YGR217WCCH1Voltage-gated high-affinity calcium channelVoltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
2629YGR218WCRM1Major karyopherinMajor karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
2630YGR219WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
2631YGR220CMRPL9Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
2632YGR221CTOS2Protein involved in localization of Cdc24p to the site of bud growthProtein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication
2633YGR222WPET54Mitochondrial inner membrane proteinMitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
2634YGR223CHSV2Phosphatidylinositol 3,5-bisphosphate-binding proteinPhosphatidylinositol 3,5-bisphosphate-binding protein; plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization
2635YGR224WAZR1Plasma membrane transporter of the major facilitator superfamilyPlasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole
2636YGR225WAMA1Activator of meiotic anaphase promoting complex (APC/C)Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest
2637YGR226CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
2638YGR227WDIE2Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferaseDolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase; located in the ER; functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1
2639YGR228WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
2640YGR229CSMI1Protein involved in the regulation of cell wall synthesisProtein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
2641YGR230WBNS1Protein of unknown functionProtein of unknown function; overexpression bypasses need for Spo12p, but not required for meiosis; BNS1 has a paralog, SPO12, that arose from the whole genome duplication
2642YGR231CPHB2Subunit of the prohibitin complex (Phb1p-Phb2p)Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
2643YGR232WNAS6Evolutionarily conserved 19S regulatory particle assembly-chaperoneAssembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6
2644YGR233CPHO81Cyclin-dependent kinase (CDK) inhibitorCyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
2645YGR234WYHB1Nitric oxide oxidoreductaseNitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
2646YGR235CMIC26Component of the MICOS complexComponent of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex
2647YGR236CSPG1Protein required for high temperature survival during stationary phaseProtein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2648YGR237CYGR237CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
2649YGR238CKEL2Protein that negatively regulates mitotic exitProtein that negatively regulates mitotic exit; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; KEL2 has a paralog, KEL1, that arose from the whole genome duplication
2650YGR239CPEX21Peroxin required for peroxisomal matrix protein targetingPeroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress; PEX21 has a paralog, PEX18, that arose from the whole genome duplication
2651YGR240CPFK1Alpha subunit of heterooctameric phosphofructokinaseAlpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
2652YGR240C-AYGR240C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
2653YGR241CYAP1802Protein of the AP180 family, involved in clathrin cage assemblyProtein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication
2654YGR242WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
2655YGR243WMPC3Highly conserved subunit of the mitochondrial pyruvate carrier (MPC)Highly conserved subunit of mitochondrial pyruvate carrier; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress; MPC3 has a paralog, MPC2, that arose from the whole genome duplication
2656YGR244CLSC2Beta subunit of succinyl-CoA ligaseBeta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
2657YGR245CSDA1Protein required for actin organization and passage through StartProtein required for actin organization and passage through Start; highly conserved nuclear protein; required for actin cytoskeleton organization; plays a critical role in G1 events; binds Nap1p; involved in 60S ribosome biogenesis
2658YGR246CBRF1TFIIIB B-related factorTFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
2659YGR247WCPD1Cyclic nucleotide phosphodiesteraseCyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
2660YGR248WSOL46-phosphogluconolactonase6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
2661YGR249WMGA1Protein similar to heat shock transcription factorProtein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
2662YGR250CRIE1Putative RNA binding proteinPutative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
2663YGR251WNOP19Ribosome biogenesis factorRibosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
2664YGR252WGCN5Catalytic subunit of ADA and SAGA histone acetyltransferase complexesCatalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
2665YGR253CPUP2Alpha 5 subunit of the 20S proteasomeAlpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
2666YGR254WENO1Enolase I, a phosphopyruvate hydrataseEnolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication
2667YGR255CCOQ6Flavin-dependent monooxygenase involved in ubiquinone biosynthesisPutative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
2668YGR256WGND26-phosphogluconate dehydrogenase (decarboxylating)6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
2669YGR257CMTM1Mitochondrial protein of the mitochondrial carrier familyMitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5′-phosphate transporter; involved in mitochondrial iron homeostasis and in activating mitochondrial Sod2p by facilitating insertion of an essential manganese cofactor
2670YGR258CRAD2Single-stranded DNA endonucleaseSingle-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
2671YGR259CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W
2672YGR260WTNA1High affinity nicotinic acid plasma membrane permeaseHigh affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
2673YGR261CAPL6Beta3-like subunit of the yeast AP-3 complexBeta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
2674YGR262CBUD32Protein kinaseProtein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription
2675YGR263CSAY1Sterol deacetylaseSterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER
2676YGR264CMES1Methionyl-tRNA synthetaseMethionyl-tRNA synthetase; forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
2677YGR265WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
2678YGR266WYGR266WProtein of unknown functionProtein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress
2679YGR267CFOL2GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesisGTP-cyclohydrolase I; catalyzes the first step in the folic acid biosynthetic pathway
2680YGR268CHUA1Cytoplasmic protein containing a zinc finger domainCytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
2681YGR269WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C
2682YGR270C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YTA7/YGR270W
2683YGR270WYTA7Protein that localizes to chromatinProtein that localizes to chromatin; has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; relocalizes to the cytosol in response to hypoxia; potentially phosphorylated by Cdc28p
2684YGR271C-AEFG1Essential protein required for maturation of 18S rRNAEssential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
2685YGR271WSLH1Putative RNA helicase related to Ski2pPutative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses
2686YGR273CYGR273CPutative protein of unknown functionPutative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication
2687YGR274CTAF1TFIID subunit, involved in RNA pol II transcription initiationTFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia
2688YGR275WRTT102Component of both the SWI/SNF and RSC chromatin remodeling complexesComponent of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress
2689YGR276CRNH703'-5' exoribonuclease3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
2690YGR277CCAB4Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC)Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
2691YGR278WCWC22Spliceosome-associated protein that is required for pre-mRNA splicingSpliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
2692YGR279CSCW4Cell wall protein with similarity to glucanasesCell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication
2693YGR280CPXR1Essential protein involved in rRNA and snoRNA maturationEssential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
2694YGR281WYOR1Plasma membrane ATP-binding cassette (ABC) transporterPlasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR)
2695YGR282CBGL2Endo-beta-1,3-glucanaseEndo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall
2696YGR283CYGR283CPutative methyltransferasePutative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication
2697YGR284CERV29Protein localized to COPII-coated vesiclesProtein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
2698YGR285CZUO1Ribosome-associated chaperoneRibosome-associated chaperone; zuotin functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
2699YGR286CBIO2Biotin synthaseBiotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
2700YGR287CIMA1Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase)Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family
2701YGR288WMAL13MAL-activator proteinMAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C
2702YGR289CMAL11High-affinity maltose transporter (alpha-glucoside transporter)High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
2703YGR290WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
2704YGR291CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2705YGR292WMAL12Maltase (alpha-D-glucosidase)Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
2706YGR293CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR294W
2707YGR294WPAU12Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
2708YGR295CCOS6Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
2709YGR296C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL07R
2710YGR296C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; contained completely within TEL07R
2711YGR296WYRF1-3Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
2712YHL001WRPL14BRibosomal 60S subunit protein L14BRibosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
2713YHL002C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF HSE1/YHL002W
2714YHL002WHSE1Subunit of the endosomal Vps27p-Hse1p complexSubunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
2715YHL003CLAG1Ceramide synthase componentCeramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enahnces invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
2716YHL004WMRP4Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
2717YHL005CYHL005CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W
2718YHL006CSHU1Component of Shu complex (aka PCSS complex)Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments
2719YHL006W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF STE20/YHL007C
2720YHL007CSTE20Cdc42p-activated signal transducing kinaseCdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
2721YHL008CYHL008CPutative protein of unknown functionPutative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
2722YHL009CYAP3Basic leucine zipper (bZIP) transcription factorBasic leucine zipper (bZIP) transcription factor
2723YHL009W-AYHL009W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
2724YHL009W-BYHL009W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
2725YHL010CETP1Protein of unknown function required for growth on ethanolPutative protein of unknown function required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences
2726YHL011CPRS35-phospho-ribosyl-1(alpha)-pyrophosphate synthetase5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
2727YHL012WYHL012WPutative UTP glucose-1-phosphate uridylyltransferasePutative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
2728YHL013COTU2Protein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
2729YHL014CYLF2Protein of unknown functionProtein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
2730YHL015WRPS20Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
2731YHL015W-AYHL015W-APutative protein of unknown functionPutative protein of unknown function
2732YHL016CDUR3Plasma membrane transporter for both urea and polyaminesPlasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
2733YHL017WYHL017WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
2734YHL018WMCO14Putative 4a-hydroxytetrahydrobiopterin dehydratasePutative 4a-hydroxytetrahydrobiopterin dehydratase; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS
2735YHL019CAPM2Protein of unknown functionProtein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
2736YHL019W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene APM2/YHL019C
2737YHL020COPI1Transcriptional regulator of a variety of genesTranscriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol
2738YHL021CAIM17Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
2739YHL022CSPO11Meiosis-specific protein that initiates meiotic recombinationMeiosis-specific protein that initiates meiotic recombination; initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation
2740YHL023CNPR3Subunit of the Iml1p/SEACIT complexSubunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
2741YHL024WRIM4Putative RNA-binding proteinPutative RNA-binding protein; required for the expression of early and middle sporulation genes
2742YHL025WSNF6Subunit of the SWI/SNF chromatin remodeling complexSubunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions
2743YHL026CYHL026CPutative protein of unknown functionPutative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
2744YHL027WRIM101Cys2His2 zinc-finger transcriptional repressorCys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
2745YHL028WWSC4Endoplasmic reticulum (ER) membrane proteinEndoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
2746YHL029COCA5Cytoplasmic protein required for replication of Brome mosaic virusCytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
2747YHL030WECM29Scaffold proteinScaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
2748YHL030W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene GOS1/YHL031C
2749YHL031CGOS1v-SNARE protein involved in Golgi transportv-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28
2750YHL032CGUT1Glycerol kinaseGlycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
2751YHL033CRPL8ARibosomal 60S subunit protein L8ARibosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
2752YHL034CSBP1Protein that binds eIF4G and has a role in repression of translationProtein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication
2753YHL034W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SBP1/YHL034C
2754YHL035CVMR1Vacuolar membrane proteinVacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication
2755YHL036WMUP3Low affinity methionine permeaseLow affinity methionine permease; similar to Mup1p
2756YHL037CYHL037CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2757YHL038CCBP2Required for splicing of the group I intron bI5 of the COB pre-mRNARequired for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
2758YHL039WEFM1Lysine methyltransferaseLysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
2759YHL040CARN1ARN family transporter for siderophore-iron chelatesARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
2760YHL041WYHL041WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2761YHL042WYHL042WProtein of unknown functionPutative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
2762YHL043WECM34Protein of unknown functionPutative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
2763YHL044WYHL044WPutative integral membrane proteinPutative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern
2764YHL045WPutative protein of unknown function; not an essential gene
2765YHL046CPAU13Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock
2766YHL046W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARN2/YHL047C
2767YHL047CARN2TransporterTransporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
2768YHL048C-AYHL048C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
2769YHL048WCOS8Endosomal protein involved in turnover of plasma membrane proteinsNuclear membrane protein; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response
2770YHL049CYHL049CPutative protein of unknown functionPutative protein of unknown function
2771YHL050CYHL050CPutative protein of unknown functionPutative protein of unknown function; potential Cdc28p substrate
2772YHL050W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2773YHR001WOSH7Oxysterol-binding proteinOxysterol-binding protein; part of family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH7 has a paralog, OSH6, that arose from the whole genome duplication
2774YHR001W-AQCR10Subunit of the ubiqunol-cytochrome c oxidoreductase complexSubunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
2775YHR002WLEU5Mitochondrial carrier proteinMitochondrial carrier protein; involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized
2776YHR003CTCD1tRNA threonylcarbamoyladenosine dehydratasetRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication
2777YHR004CNEM1Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzymeProbable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
2778YHR005CGPA1Subunit of the G protein involved in pheromone responseSubunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress
2779YHR005C-ATIM10Essential protein of the mitochondrial intermembrane spaceEssential protein of the mitochondrial intermembrane space; forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
2780YHR006WSTP2Transcription factorTranscription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
2781YHR007CERG11Lanosterol 14-alpha-demethylaseLanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
2782YHR007C-AYHR007C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
2783YHR008CSOD2Mitochondrial manganese superoxide dismutaseMitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity; phosphorylated
2784YHR009CTDA3Putative oxidoreductase involved in late endosome to Golgi transportPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
2785YHR010WRPL27ARibosomal 60S subunit protein L27ARibosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
2786YHR011WDIA4Probable mitochondrial seryl-tRNA synthetaseProbable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth
2787YHR012WVPS29Subunit of the membrane-associated retromer complexSubunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
2788YHR013CARD1Subunit of protein N-terminal acetyltransferase NatASubunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress
2789YHR014WSPO13Meiotic regulatorMeiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells
2790YHR015WMIP6Putative RNA-binding proteinPutative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
2791YHR016CYSC84Actin-binding proteinActin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
2792YHR017WYSC83Non-essential mitochondrial protein of unknown functionNon-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
2793YHR018CARG4Argininosuccinate lyaseArgininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway
2794YHR019CDED81Cytosolic asparaginyl-tRNA synthetaseCytosolic asparaginyl-tRNA synthetase; required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
2795YHR020WYHR020WProlyl-tRNA synthetaseProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
2796YHR021CRPS27BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
2797YHR021W-AECM12Putative protein of unknown functionPutative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
2798YHR022CYHR022CPutative protein of unknown functionPutative protein of unknown function; YHR022C is not an essential gene
2799YHR022C-AYHR022C-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
2800YHR023WMYO1Type II myosin heavy chainType II myosin heavy chain; required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
2801YHR024CMAS2Alpha subunit of the mitochondrial processing protease (MPP)Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
2802YHR025WTHR1Homoserine kinaseHomoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway
2803YHR026WVMA16Subunit c'' of the vacuolar ATPaseSubunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
2804YHR027CRPN1Non-ATPase base subunit of the 19S RP of the 26S proteasomeNon-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle
2805YHR028CDAP2Dipeptidyl aminopeptidaseDipeptidyl aminopeptidase; synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
2806YHR028W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF DAP2/YHR028C
2807YHR029CYHI9Protein of unknown functionProtein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production
2808YHR030CSLT2Serine/threonine MAP kinaseSerine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to phagophore assembly site (PAS); plays a role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway; SLT2 has a paralog, KDX1, that arose from the whole genome duplication
2809YHR031CRRM3DNA helicase involved in rDNA replication and Ty1 transpositionDNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
2810YHR032C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032W-A
2811YHR032WERC1Member of the multi-drug and toxin extrusion (MATE) familyMember of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
2812YHR032W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032C-A
2813YHR033WYHR033WPutative protein of unknown functionPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication
2814YHR034CPIH1Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1)Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
2815YHR035WNEL1Activator of Sar1p GTPase activityActivator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene
2816YHR036WBRL1Essential nuclear envelope/ER integral membrane proteinEssential nuclear envelope integral membrane protein; identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
2817YHR037WPUT2Delta-1-pyrroline-5-carboxylate dehydrogenaseDelta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism
2818YHR038WRRF1Mitochondrial ribosome recycling factorMitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
2819YHR039CMSC7Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
2820YHR039C-AVMA10Subunit G of the V1 peripheral membrane domain of V-ATPaseSubunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
2821YHR040WBCD1Essential protein required for the accumulation of box C/D snoRNAEssential protein required for the accumulation of box C/D snoRNA
2822YHR041CSRB2Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
2823YHR042WNCP1NADP-cytochrome P450 reductaseNADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
2824YHR043CDOG22-deoxyglucose-6-phosphate phosphatase2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
2825YHR044CDOG12-deoxyglucose-6-phosphate phosphatase2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
2826YHR045WYHR045WPutative protein of unknown functionPutative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
2827YHR046CINM1Inositol monophosphataseInositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
2828YHR047CAAP1Arginine/alanine amino peptidaseArginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication
2829YHR048WYHK8Presumed antiporter of the major facilitator superfamilyPresumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
2830YHR049C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2831YHR049WFSH1Putative serine hydrolasePutative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
2832YHR050WSMF2Divalent metal ion transporter involved in manganese homeostasisDivalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
2833YHR050W-AYHR050W-AProtein of unknown functionProtein of unknown function; identified by expression profiling and mass spectrometry
2834YHR051WCOX6Subunit VI of cytochrome c oxidase (Complex IV)Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
2835YHR052WCIC1Essential protein that interacts with proteasome componentsEssential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles
2836YHR052W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CUP1-1
2837YHR053CCUP1-1MetallothioneinMetallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-1 has a paralog, CUP1-2, that arose from a segmental duplication
2838YHR054CYHR054CPutative protein of unknown functionPutative protein of unknown function; partial duplicate of RSC30/YHR056C, truncated remnant of segmental duplication
2839YHR054W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CUP1-2
2840YHR055CCUP1-2MetallothioneinMetallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-2 has a paralog, CUP1-1, that arose from a segmental duplication
2841YHR056CRSC30Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; null mutants are osmosensitive; RSC30 has a paralog, RSC3, that arose from the whole genome duplication
2842YHR056W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF RSC30/YHR056C
2843YHR057CCPR2Peptidyl-prolyl cis-trans isomerase (cyclophilin)Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR2 has a paralog, CPR5, that arose from the whole genome duplication; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
2844YHR058CMED6Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
2845YHR059WFYV4Protein of unknown functionProtein of unknown function; required for survival upon exposure to K1 killer toxin
2846YHR060WVMA22Protein that is required for vacuolar H+-ATPase (V-ATPase) functionProtein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
2847YHR061CGIC1Protein involved in initiation of budding and cellular polarizationProtein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication
2848YHR062CRPP1Subunit of both RNase MRP and nuclear RNase PSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
2849YHR063CPAN52-dehydropantoate 2-reductase2-dehydropantoate 2-reductase; part of the pantothenic acid pathway, structurally homologous to E. coli panE
2850YHR063W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SSZ1/YHR064C
2851YHR064CSSZ1Hsp70 protein that interacts with Zuo1p (a DnaJ homolog)Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
2852YHR065CRRP3Protein involved in rRNA processingProtein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
2853YHR066WSSF1Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
2854YHR067WHTD2Mitochondrial 3-hydroxyacyl-thioester dehydrataseMitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
2855YHR068WDYS1Deoxyhypusine synthaseDeoxyhypusine synthase; catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
2856YHR069CRRP4Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
2857YHR069C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF TRM5/YHR070W
2858YHR070C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene TRM5/YHR070W
2859YHR070WTRM5tRNA(m(1)G37)methyltransferasetRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya
2860YHR071C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ERG7/YHR072W
2861YHR071WPCL5CyclinCyclin; interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
2862YHR072WERG7Lanosterol synthaseLanosterol synthase; an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
2863YHR072W-ANOP10Subunit of box H/ACA snoRNP complexSubunit of box H/ACA snoRNP complex; required for pseudouridylation and processing of pre-18S rRNA
2864YHR073C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry
2865YHR073WOSH3Member of an oxysterol-binding protein familyMember of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
2866YHR073W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry
2867YHR074WQNS1Glutamine-dependent NAD(+) synthetaseGlutamine-dependent NAD(+) synthetase; essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
2868YHR075CPPE1Protein with carboxyl methyl esterase activityProtein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein
2869YHR076WPTC7Type 2C serine/threonine protein phosphatase (PP2C)Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p
2870YHR077CNMD2Protein involved in the nonsense-mediated mRNA decay (NMD) pathwayProtein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
2871YHR078WYHR078WHigh osmolarity-regulated gene of unknown functionHigh osmolarity-regulated gene of unknown function
2872YHR079CIRE1Serine-threonine kinase and endoribonucleaseSerine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
2873YHR079C-ASAE3Meiosis-specific protein involved in meiotic recombinationMeiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p
2874YHR080CLAM4Sterol-binding protein that localizes to puncta in the cortical ERProtein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
2875YHR081WLRP1Nuclear exosome-associated nucleic acid binding proteinNuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination
2876YHR082CKSP1Serine/threonine protein kinaseSerine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
2877YHR083WSAM35Component of the sorting and assembly machinery (SAM) complexComponent of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
2878YHR084WSTE12Transcription factor that is activated by a MAPK signaling cascadeTranscription factor that is activated by a MAPK signaling cascade; activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth
2879YHR085WIPI1Component of the Rix1 complex and possibly pre-replicative complexesComponent of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
2880YHR086WNAM8RNA binding protein, component of the U1 snRNP proteinRNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
2881YHR086W-AYHR086W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
2882YHR087WRTC3Protein of unknown function involved in RNA metabolismProtein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress
2883YHR088WRPF1Protein involved in assembly and export of the large ribosomal subunitProtein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
2884YHR089CGAR1Protein component of the H/ACA snoRNP pseudouridylase complexProtein component of the H/ACA snoRNP pseudouridylase complex; involved in the modification and cleavage of the 18S pre-rRNA
2885YHR090CYNG2Subunit of NuA4, an essential histone acetyltransferase complexSubunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; relocalizes to the cytosol in response to hypoxia; similar to human tumor suppressor ING1 and its isoforms ING4 and ING5
2886YHR091CMSR1Mitochondrial arginyl-tRNA synthetaseMitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication
2887YHR092CHXT4High-affinity glucose transporterHigh-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication
2888YHR093WAHT1Putative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region
2889YHR094CHXT1Low-affinity glucose transporter of the major facilitator superfamilyLow-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
2890YHR095WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2891YHR096CHXT5Hexose transporter with moderate affinity for glucoseHexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication
2892YHR097CYHR097CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication
2893YHR098CSFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coatComponent of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat; COPII coat is required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
2894YHR099WTRA1Subunit of SAGA and NuA4 histone acetyltransferase complexesSubunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation
2895YHR100CGEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase)Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase); dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
2896YHR101CBIG1Integral membrane protein of the endoplasmic reticulumIntegral membrane protein of the endoplasmic reticulum; required for normal content of cell wall beta-1,6-glucan
2897YHR102WKIC1Protein kinase of the PAK/Ste20 family, required for cell integrityProtein kinase of the PAK/Ste20 family, required for cell integrity; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; part of the RAM network that regulates cellular polarity and morphogenesis
2898YHR103WSBE22Protein involved in bud growthProtein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication
2899YHR104WGRE3Aldose reductaseAldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
2900YHR105WYPT35Endosomal protein of unknown functionEndosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
2901YHR106WTRR2Mitochondrial thioredoxin reductaseMitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
2902YHR107CCDC12Component of the septin ring that is required for cytokinesisComponent of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
2903YHR108WGGA2Protein that regulates Arf1p, Arf2p to facilitate Golgi traffickingProtein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication
2904YHR109WCTM1Cytochrome c lysine methyltransferaseCytochrome c lysine methyltransferase; trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth
2905YHR110WERP5Protein with similarity to Emp24p and Erv25pProtein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
2906YHR111WUBA4E1-like protein that activates Urm1p before urmylationE1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
2907YHR112CYHR112CProtein of unknown functionProtein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
2908YHR113WAPE4Cytoplasmic aspartyl aminopeptidase with possible vacuole functionCytoplasmic aspartyl aminopeptidase with possible vacuole function; Cvt pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
2909YHR114WBZZ1SH3 domain protein implicated in regulating actin polymerizationSH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
2910YHR115CDMA1Ubiquitin-protein ligase (E3)Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
2911YHR116WCOX23Protein that functions in mitochondrial copper homeostasisProtein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
2912YHR117WTOM71Mitochondrial outer membrane proteinMitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
2913YHR118CORC6Subunit of the origin recognition complex (ORC)Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
2914YHR119WSET1Histone methyltransferase, subunit of the COMPASS (Set1C) complexHistone methyltransferase, subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3K4; Set1p-dependent H3K4 trimethylation recruits Nrd1p, allowing efficient termination of snoRNAs and cryptic unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway; modulates histone acetylation levels in promoter proximal regions to ensure efficient Nrd1p-dependent termination; required in transcriptional silencing near telomeres and at silent mating type loci; has a SET domain
2915YHR120WMSH1DNA-binding protein of the mitochondriaDNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
2916YHR121WLSM12Protein of unknown function that may function in RNA processingProtein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
2917YHR122WCIA2Component of cytosolic iron-sulfur protein assembly (CIA) machineryComponent of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
2918YHR123WEPT1sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferasesn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
2919YHR124WNDT80Meiosis-specific transcription factorMeiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)
2920YHR125WYHR125WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2921YHR126CANS1Putative GPI proteinPutative GPI protein; transcription dependent upon Azf1p
2922YHR127WYHR127WProtein of unknown functionProtein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
2923YHR128WFUR1Uracil phosphoribosyltransferaseUracil phosphoribosyltransferase; synthesizes UMP from uracil; involved in the pyrimidine salvage pathway
2924YHR129CARP1Actin-related protein of the dynactin complexActin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin
2925YHR130CYHR130CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2926YHR131CYHR131CPutative protein of unknown functionPutative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication
2927YHR131W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YHR131C
2928YHR132CECM14Putative metalloprotease with similarity to zinc carboxypeptidasesPutative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly
2929YHR132W-AIGO2Protein required for initiation of G0 programProtein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication
2930YHR133CNSG1Protein involved in regulation of sterol biosynthesisProtein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication
2931YHR134WWSS1SUMO-ligase and SUMO-targeted metalloproteaseMetalloprotease involved in DNA repair, removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; sumoylated protein localizing to the nuclear periphery of mother cells; localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; activated by DNA binding
2932YHR135CYCK1Palmitoylated plasma membrane-bound casein kinase I (CK1) isoformPalmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
2933YHR136CSPL2Protein with similarity to cyclin-dependent kinase inhibitorsProtein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
2934YHR137C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene ARO9/YHR137W; identified by gene-trapping, expression analysis, and genome-wide homology searching
2935YHR137WARO9Aromatic aminotransferase IIAromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism
2936YHR138CYHR138CProtein of unknown functionProtein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
2937YHR139CSPS100Protein required for spore wall maturationProtein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin; SPS100 has a paralog, YGP1, that arose from the whole genome duplication
2938YHR139C-AYHR139C-APutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2939YHR140WYHR140WPutative integral membrane protein of unknown functionPutative integral membrane protein of unknown function
2940YHR141CRPL42BRibosomal 60S subunit protein L42BRibosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication
2941YHR142WCHS7Protein of unknown functionProtein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
2942YHR143WDSE2Daughter cell-specific secreted protein with similarity to glucanasesDaughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
2943YHR143W-ARPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and IIIRNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
2944YHR144CDCD1Deoxycytidine monophosphate (dCMP) deaminaseDeoxycytidine monophosphate (dCMP) deaminase; involved in dUMP and dTMP biosynthesis; expression is NOT cell cycle regulated
2945YHR145CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2946YHR146WCRP1Protein that binds to cruciform DNA structuresProtein that binds to cruciform DNA structures; CRP1 has a paralog, MDG1, that arose from the whole genome duplication
2947YHR147CMRPL6Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
2948YHR148WIMP3Component of the SSU processomeComponent of the SSU processome; SSU processome is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
2949YHR149CSKG6Integral membrane proteinIntegral membrane protein; localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p; SKG6 has a paralog, TOS2, that arose from the whole genome duplication
2950YHR150WPEX28Peroxisomal integral membrane peroxinPeroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p
2951YHR151CMTC6Protein of unknown functionProtein of unknown function; mtc6 is synthetically sick with cdc13-1
2952YHR152WSPO12Nucleolar protein of unknown functionNucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication
2953YHR153CSPO16Meiosis-specific protein involved in synaptonemal complex assemblyMeiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
2954YHR154WRTT107Protein implicated in Mms22-dependent DNA repair during S phaseProtein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
2955YHR155WLAM1Putative sterol transfer proteinMitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
2956YHR156CLIN1Non-essential component of U5 snRNPNon-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
2957YHR157WREC104Protein involved in early stages of meiotic recombinationProtein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
2958YHR158CKEL1Protein required for proper cell fusion and cell morphologyProtein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; KEL1 has a paralog, KEL2, that arose from the whole genome duplication
2959YHR159WTDA11Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
2960YHR160CPEX18PeroxinPeroxin; required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p; PEX18 has a paralog, PEX21, that arose from the whole genome duplication
2961YHR161CYAP1801Protein of the AP180 family, involved in clathrin cage assemblyProtein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
2962YHR162WMPC2Highly conserved subunit of the mitochondrial pyruvate carrier (MPC)Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Mpc1p and either Mpc2p or Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; MPC2 has a paralog, FMP43, that arose from the whole genome duplication
2963YHR163WSOL36-phosphogluconolactonase6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
2964YHR164CDNA2Tripartite DNA replication factorTripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress
2965YHR165CPRP8Component of U4/U6-U5 snRNP complexComponent of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in its human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome.
2966YHR165W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps ORF PRP8/YHR165C
2967YHR166CCDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
2968YHR167WTHP2Subunit of the THO and TREX complexesSubunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
2969YHR168WMTG2Putative GTPasePutative GTPase; member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
2970YHR169WDBP8ATPase, putative RNA helicase of the DEAD-box familyATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esf2p
2971YHR170WNMD3Protein involved in nuclear export of the large ribosomal subunitProtein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
2972YHR171WATG7Autophagy-related protein and dual specificity member of the E1 familyAutophagy-related protein and dual specificity member of the E1 family; mediates the attachment of Atg12p to Atg5p and Atg8p to phosphatidylethanolamine which are required steps in autophagosome formation; E1 enzymes are also known as ubiquitin-activating enzymes; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
2973YHR172WSPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complexComponent of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
2974YHR173CYHR173CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
2975YHR174WENO2Enolase II, a phosphopyruvate hydrataseEnolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication
2976YHR175WCTR2Low-affinity copper transporter of the vacuolar membranePutative low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation; regulated by nonsense-mediated mRNA decay pathway
2977YHR175W-AYHR175W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
2978YHR176WFMO1Flavin-containing monooxygenaseFlavin-containing monooxygenase; localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins
2979YHR177WROF1Putative transcription factor containing a WOPR domainPutative transcription factor containing a WOPR domain; binds DNA in vitro; similar to C. albicans Wor1p transcription factor that regulates white-opaque switching; overexpression causes a cell cycle delay or arrest
2980YHR178WSTB5Transcription factorTranscription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro
2981YHR179WOYE2Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN)Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
2982YHR180C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YHR180W-A
2983YHR180WYHR180WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
2984YHR180W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YHR180C-B and long terminal repeat YHRCsigma3
2985YHR181WSVP26Integral membrane protein of the early Golgi apparatus and ERIntegral membrane protein of the early Golgi apparatus and ER; involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment
2986YHR182C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF GND1/YHR183W
2987YHR182WYHR182WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
2988YHR183WGND16-phosphogluconate dehydrogenase (decarboxylating)6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
2989YHR184WSSP1Protein involved in the control of meiotic nuclear divisionProtein involved in the control of meiotic nuclear division; also involved in the coordination of meiosis with spore formation; transcription is induced midway through meiosis
2990YHR185CPFS1Sporulation protein required for prospore membrane formationSporulation protein required for prospore membrane formation; required for prospore membrane formation at selected spindle poles; ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
2991YHR186CKOG1Subunit of TORC1Subunit of TORC1; TORC1 is a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
2992YHR187WIKI1Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplexSubunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
2993YHR188CGPI16Subunit of the glycosylphosphatidylinositol transamidase complexSubunit of the glycosylphosphatidylinositol transamidase complex; transmembrane protein; adds GPIs to newly synthesized proteins; human PIG-Tp homolog
2994YHR189WPTH1One of two mitochondrially-localized peptidyl-tRNA hydrolasesOne of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2
2995YHR190WERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase)Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
2996YHR191CCTF8Subunit of a complex with Ctf18pSubunit of a complex with Ctf18p; shares some subunits with Replication Factor C; required for sister chromatid cohesion
2997YHR192WLNP1Lunapark family member involved in ER network formationLunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS
2998YHR193CEGD2Alpha subunit of the nascent polypeptide-associated complex (NAC)Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes
2999YHR193C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified ORF MDM31/YHR194W
3000YHR194WMDM31Mitochondrial protein that may have a role in phospholipid metabolismMitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
3001YHR195WNVJ1Nuclear envelope proteinNuclear envelope protein; anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
3002YHR196WUTP9Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
3003YHR197WRIX1Component of the Rix1 complex and possibly pre-replicative complexesComponent of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
3004YHR198CAIM18Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
3005YHR199CAIM46Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
3006YHR199C-ANBL1Subunit of the conserved chromosomal passenger complex (CPC)Subunit of the conserved chromosomal passenger complex (CPC); complex regulates mitotic chromosome segregation; not required for the kinase activity of the complex; mediates the interaction of Sli15p and Bir1p; other complex members are Ipl1p, Sli15p, and Bir1p
3007YHR200WRPN10Non-ATPase base subunit of the 19S RP of the 26S proteasomeNon-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
3008YHR201CPPX1ExopolyphosphataseExopolyphosphatase; hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix
3009YHR202WYHR202WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
3010YHR203CRPS4BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
3011YHR204WMNL1Alpha-1,2-specific exomannosidase of the endoplasmic reticulumAlpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation
3012YHR205WSCH9AGC family protein kinaseAGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
3013YHR206WSKN7Nuclear response regulator and transcription factorNuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
3014YHR207CSET5Methyltransferase involved in methylation of histone H4 Lys5, -8, -12Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
3015YHR208WBAT1Mitochondrial branched-chain amino acid (BCAA) aminotransferaseMitochondrial branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA biosynthesis; homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase; BAT1 has a paralog, BAT2, that arose from the whole genome duplication
3016YHR209WCRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasisS-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
3017YHR210CYHR210CPutative aldose 1-epimerase superfamily proteinPutative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
3018YHR211WFLO5Lectin-like cell wall protein (flocculin) involved in flocculationLectin-like cell wall protein (flocculin) involved in flocculation; binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; FLO5 has a paralog, FLO1, that arose from a segmental duplication
3019YHR212CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YHR212C has a paralog, YAR060C, that arose from a segmental duplication
3020YHR212W-AYHR212W-APseudogenic fragment with similarity to flocculinsPseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR212W-A has a paralog, YAR061W, that arose from a segmental duplication
3021YHR213WYHR213WPseudogenic fragment with similarity to flocculinsPseudogenic fragment with similarity to flocculins; YHR213W has a paralog, YAR062W, that arose from a segmental duplication
3022YHR213W-AYHR213W-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3023YHR213W-BYHR213W-BPseudogenic fragment with similarity to flocculinsPseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR213W-B has a paralog, YAR064W, that arose from a segmental duplication
3024YHR214C-BYHR214C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes; YHR214C-B has a paralog, YAR070C, that arose from a segmental duplication
3025YHR214C-CYHR214C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
3026YHR214C-DYHR214C-DPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR214C-D has a paralog, YAR069C, that arose from a segmental duplication
3027YHR214C-EYHR214C-EPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3028YHR214WYHR214WPutative protein of unknown functionPutative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein; YHR214W has a paralog, YAR066W, that arose from a segmental duplication
3029YHR214W-ADubious open reading frame; induced by zinc deficiency; YHR214W-A has a paralog, YAR068W, that arose from a segmental duplication
3030YHR215WPHO12One of three repressible acid phosphatasesOne of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; pregulated by phosphate starvation; PHO12 has a paralog, PHO11, that arose from a segmental duplication
3031YHR216WIMD2Inosine monophosphate dehydrogenaseInosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication
3032YHR217CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; located in the telomeric region TEL08R
3033YHR218WYHR218WHelicase-like protein encoded within the telomeric Y' elementHelicase-like protein encoded within the telomeric Y' element
3034YHR218W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely within telomere TEL08L
3035YHR219C-ADubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3036YHR219WYHR219WPutative protein of unknown function with similarity to helicasesPutative protein of unknown function with similarity to helicases; located in the telomere region on the right arm of chromosome VIII
3037YIL001WYIL001WPutative protein of unknown functionPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
3038YIL002CINP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatasePhosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
3039YIL002W-ACMI7Putative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
3040YIL003WCFD1Highly conserved iron-sulfur cluster binding proteinHighly conserved iron-sulfur cluster binding protein; localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
3041YIL004CBET1Type II membrane protein required for vesicular transportType II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
3042YIL005WEPS1ER protein with chaperone and co-chaperone activityER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family
3043YIL006WYIA6Mitochondrial NAD+ transporterMitochondrial NAD+ transporter; involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs; YIA6 has a paralog, YEA6, that arose from the whole genome duplication
3044YIL007CNAS2Evolutionarily conserved 19S regulatory particle assembly-chaperoneProteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress
3045YIL008WURM1Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAsUbiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
3046YIL009C-AEST3Component of the telomerase holoenzymeComponent of the telomerase holoenzyme; involved in telomere replication
3047YIL009WFAA3Long chain fatty acyl-CoA synthetaseLong chain fatty acyl-CoA synthetase; activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
3048YIL010WDOT5Nuclear thiol peroxidaseNuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth
3049YIL011WTIR3Cell wall mannoproteinCell wall mannoprotein; member of Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; TIR3 has a paralog, TIR2, that arose from the whole genome duplication
3050YIL012WYIL012WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3051YIL013CPDR11ATP-binding cassette (ABC) transporterATP-binding cassette (ABC) transporter; multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth; PDR11 has a paralog, AUS1, that arose from the whole genome duplication
3052YIL014C-AYIL014C-APutative protein of unknown functionPutative protein of unknown function
3053YIL014WMNT3Alpha-1,3-mannosyltransferaseAlpha-1,3-mannosyltransferase; adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation
3054YIL015WBAR1Aspartyl proteaseAspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
3055YIL016WSNL1Ribosome-associated proteinRibosome-associated protein; proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein
3056YIL017CVID28GID Complex subunit, serves as adaptor for regulatory subunit Vid24pGID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm
3057YIL018WRPL2BRibosomal 60S subunit protein L2BRibosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
3058YIL019WFAF1Protein required for pre-rRNA processingProtein required for pre-rRNA processing; also required for 40S ribosomal subunit assembly
3059YIL020CHIS6Enzyme that catalyzes the fourth step in the histidine pathwayEnzyme that catalyzes the fourth step in the histidine pathway; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
3060YIL020C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps origin of replication ARS918, partially overlaps ORF RPB3/YIL021W
3061YIL021C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene RPB3/YIL021W; identified by gene-trapping, expression analysis, and genome-wide homology searching
3062YIL021WRPB3RNA polymerase II third largest subunit B44RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
3063YIL022WTIM44Essential component of the TIM23 complexEssential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane
3064YIL023CYKE4Zinc transporterZinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family
3065YIL024CYIL024CPutative protein of unknown functionPutative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p
3066YIL025CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3067YIL026CIRR1Subunit of the cohesin complexSubunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability
3068YIL027CEMC5Member of conserved ER transmembrane complexMember of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
3069YIL028WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3070YIL029CYIL029CPutative protein of unknown functionPutative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has a paralog, YPR071W, that arose from a single-locus duplication
3071YIL029W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SSM4/YIL030C
3072YIL030CSSM4Membrane-embedded ubiquitin-protein ligaseUbiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
3073YIL030W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF ULP2/YIL031W
3074YIL031WULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteinsPeptidase that deconjugates Smt3/SUMO-1 peptides from proteins; plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
3075YIL032CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3076YIL033CBCY1Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA)Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
3077YIL034CCAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p)Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
3078YIL035CCKA1Alpha catalytic subunit of casein kinase 2 (CK2)Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
3079YIL036WCST6Basic leucine zipper (bZIP) transcription factor from ATF/CREB familyBasic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication
3080YIL037CPRM2Pheromone-regulated proteinPheromone-regulated protein; predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
3081YIL038CNOT3Component of the CCR4-NOT core complex, involved in mRNA decappingSubunit of CCR4-NOT global transcriptional regulator; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers
3082YIL039WTED1GPI-glycan remodelaseConserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
3083YIL040WAPQ12Nuclear envelope/ER integral membrane proteinProtein required for nuclear envelope morphology; nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
3084YIL041WGVP36BAR domain protein that localizes to early and late Golgi vesiclesBAR domain protein that localizes to early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis
3085YIL042CPKP1Mitochondrial protein kinaseMitochondrial protein kinase; involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p
3086YIL043CCBR1Cytochrome b reductaseMicrosomal cytochrome b reductase; not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
3087YIL044CAGE2ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effectorADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
3088YIL045WPIG2Putative type-1 protein phosphatase targeting subunitPutative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication
3089YIL046WMET30F-box protein containing five copies of the WD40 motifF-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p
3090YIL046W-AYIL046W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
3091YIL047CSYG1Plasma membrane protein of unknown functionPlasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
3092YIL047C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SYG1/YIL047C
3093YIL048WNEO1Phospholipid translocase (flippase), role in phospholipid asymmetry oPutative aminophospholipid translocase (flippase); involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
3094YIL049WDFG10Probable polyprenol reductaseProbable polyprenol reductase; catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human ortholog SRD5A3 confer CDG (Congenital Disorders of Glycosylation)
3095YIL050WPCL7Pho85p cyclin of the Pho80p subfamilyPho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
3096YIL051CMMF1Mitochondrial protein required for transamination of isoleucineMitochondrial protein required for transamination of isoleucine; but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; induction of expression in response to stress is mediated by a Hog1p-regulated antisense RNA and gene looping; interacts genetically with mitochondrial ribosomal protein genes; MMF1 has a paralog, HMF1, that arose from the whole genome duplication
3097YIL052CRPL34BRibosomal 60S subunit protein L34BRibosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
3098YIL053WGPP1Constitutively expressed DL-glycerol-3-phosphate phosphataseConstitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication
3099YIL054WYIL054WProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
3100YIL055CYIL055CPutative protein of unknown functionPutative protein of unknown function
3101YIL056WVHR1Transcriptional activatorTranscriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
3102YIL057CRGI2Protein of unknown functionProtein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication
3103YIL058WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3104YIL059CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W
3105YIL060WYIL060WMitochondrial protein of unknown functionMitochondrial protein of unknown function; required for respiratory growth; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
3106YIL061CSNP1Component of U1 snRNP required for mRNA splicing via spliceosomeComponent of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
3107YIL062CARC15Subunit of the ARP2/3 complexSubunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity
3108YIL063CYRB2Protein of unknown functionProtein of unknown function; involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; relocalizes to the cytosol in response to hypoxia; not essential for viability
3109YIL064WEFM4Lysine methyltransferaseLysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
3110YIL065CFIS1Protein involved in mitochondrial fission and peroxisome abundanceProtein involved in mitochondrial fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
3111YIL066CRNR3Minor isoform of large subunit of ribonucleotide-diphosphate reductaseMinor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication
3112YIL066W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YIL067C
3113YIL067CYIL067CUncharacterized protein of unknown functionUncharacterized protein of unknown function
3114YIL068CSEC6Essential 88kDa subunit of the exocyst complexEssential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
3115YIL068W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF SEC6/YIL068C
3116YIL069CRPS24BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication
3117YIL070CMAM33Specific translational activator for the mitochondrial COX1 mRNAAcidic protein of the mitochondrial matrix; involved in oxidative phosphorylation; related to the human complement receptor gC1q-R
3118YIL071CPCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
3119YIL071W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF PCI8/YIL071C
3120YIL072WHOP1Meiosis-specific protein required for chromosome synapsisMeiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein-DNA filaments
3121YIL073CSPO22Meiosis-specific protein essential for chromosome synapsisMeiosis-specific protein essential for chromosome synapsis; involved in completion of nuclear divisions during meiosis; induced early in meiosis
3122YIL074CSER333-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication
3123YIL075CRPN2Subunit of the 26S proteasomeSubunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
3124YIL076WSEC28Epsilon-COP subunit of the coatomerEpsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
3125YIL077CRCI37Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
3126YIL078WTHS1Threonyl-tRNA synthetaseThreonyl-tRNA synthetase; essential cytoplasmic protein
3127YIL079CAIR1Zinc knuckle proteinZinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome
3128YIL082WRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
3129YIL082W-AYIL082W-ARetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
3130YIL083CCAB2Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC)Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
3131YIL084CSDS3Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes
3132YIL085CKTR7Putative mannosyltransferase involved in protein glycosylationPutative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
3133YIL086CYIL086CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3134YIL087CAIM19Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth
3135YIL088CAVT7Putative transporterPutative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
3136YIL089WYIL089WProtein of unknown function found in the ER and vacuole lumenProtein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking
3137YIL090WICE2Integral ER membrane protein with type-III transmembrane domainsIntegral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
3138YIL091CUTP25Nucleolar proteinNucleolar protein; required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
3139YIL092WYIL092WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
3140YIL093CRSM25Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
3141YIL094CLYS12Homo-isocitrate dehydrogenaseHomo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
3142YIL095WPRK1Protein serine/threonine kinaseProtein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication
3143YIL096CBMT5Methyltransferase required for m3U2634 methylation of the 25S rRNAMethyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
3144YIL097WFYV10Subunit of GID complexSubunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin
3145YIL098CFMC1Mitochondrial matrix proteinMitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
3146YIL099WSGA1Intracellular sporulation-specific glucoamylaseIntracellular sporulation-specific glucoamylase; involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation
3147YIL100C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3148YIL100WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A
3149YIL101CXBP1Transcriptional repressorTranscriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
3150YIL102CYIL102CPutative protein of unknown functionPutative protein of unknown function
3151YIL102C-AYIL102C-APutative protein of unknown functionPutative protein of unknown function; identified based on comparisons of the genome sequences of six Saccharomyces species
3152YIL103WDPH1Protein required for synthesis of diphthamideProtein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
3153YIL104CSHQ1Chaperone proteinChaperone protein; required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing; functions as an RNA mimic; forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones; relocalizes to the cytosol in response to hypoxia
3154YIL105CSLM1Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2pPhosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
3155YIL105W-AYIL105W-AProtein of unknown functionProtein of unknown function; completely overlaps the verified gene SLM1; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; mRNA identified as translated by ribosome profiling data
3156YIL106WMOB1Component of the mitotic exit networkComponent of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
3157YIL107CPFK266-phosphofructo-2-kinase6-phosphofructo-2-kinase; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
3158YIL108WYIL108WPutative metalloendopeptidasePutative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
3159YIL109CSEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coatComponent of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication
3160YIL110WHPM1AdoMet-dependent methyltransferaseAdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
3161YIL111WCOX5BSubunit Vb of cytochrome c oxidaseSubunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
3162YIL112WHOS4Subunit of the Set3 complexSubunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate
3163YIL113WSDP1Stress-inducible dual-specificity MAP kinase phosphataseStress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication
3164YIL114CPOR2Putative mitochondrial porin (voltage-dependent anion channel)Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication
3165YIL115CNUP159FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
3166YIL115W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUP159/YIL115C
3167YIL116WHIS5Histidinol-phosphate aminotransferaseHistidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
3168YIL117CPRM5Pheromone-regulated protein, predicted to have 1 transmembrane segmentPheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication
3169YIL118WRHO3Non-essential small GTPase of the Rho/Rac family of Ras-like proteinsNon-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
3170YIL119CRPI1Transcription factor, allelic differences between S288C and Sigma1278bTranscription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF
3171YIL120WQDR1Multidrug transporter of the major facilitator superfamilyMultidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in spore wall assembly; sequence similarity to DTR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication
3172YIL121WQDR2Plasma membrane transporter of the major facilitator superfamilyPlasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
3173YIL122WPOG1DNA-binding transcriptional activatorNuclear chromatin-associated protein of unknown function; may have a role in cell cycle regulation; overexpression promotes recovery from pheromone induced arrest and suppresses the stress sensitivity caused by a mutation in the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; phsosphoylated form may be ubiquitinated by Dma2p; potential Cdc28p substrate; SBF regulated
3174YIL123WSIM1Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p)Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p); may participate in DNA replication; promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated; SIM1 has a paralog, SUN4, that arose from the whole genome duplication
3175YIL124WAYR1Bifunctional triacylglycerol lipase and 1-acyl DHAP reductaseBifunctional triacylglycerol lipase and 1-acyl DHAP reductase; NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase involved in phosphatidic acid biosynthesis; lipid droplet triacylglycerol lipase involved in the mobilization of non-polar lipids; found in lipid particles, the endoplasmic reticulum and the mitochondrial outer membrane; required for spore germination; role in cell wall biosynthesis; capable of metabolizing steroid hormones; oleic acid inducible
3176YIL125WKGD1Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complexSubunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
3177YIL126WSTH1ATPase component of the RSC chromatin remodeling complexATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p
3178YIL127CRRT14Putative protein of unknown functionPutative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
3179YIL128WMET18Component of cytosolic iron-sulfur protein assembly (CIA) machineryComponent of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
3180YIL129CTAO3Component of the RAM signaling networkComponent of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
3181YIL130WASG1Zinc cluster protein proposed to be a transcriptional regulatorZinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance
3182YIL131CFKH1Forkhead family transcription factorForkhead family transcription factor; minor role in expression of G2/M phase genes; negatively regulates transcription elongation; positive role in chromatin silencing at HML, HMR; facilitates clustering and activation of early-firing replication origins; binds to recombination enhancer near HML, regulates donor preference during mating-type switching; relocalizes to cytosol in response to hypoxia; FKH1 has a paralog, FKH2, that arose from the whole genome duplication
3183YIL132CCSM2Component of Shu complex (aka PCSS complex)Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair,; Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
3184YIL133CRPL16ARibosomal 60S subunit protein L16ARibosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
3185YIL134C-AYIL134C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
3186YIL134WFLX1Mitochondrial flavin adenine dinucleotide transporterProtein required for transport of flavin adenine dinucleotide (FAD); a synthesis product of riboflavin, across the mitochondrial membrane
3187YIL135CVHS2Regulator of septin dynamicsRegulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication
3188YIL136WOM45Mitochondrial outer membrane protein of unknown functionMitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress
3189YIL137CTMA108Ribosome-associated, nascent chain binding factorRibosome-associated protein that is involved in ribosome biogenesis; putative metalloprotease
3190YIL138CTPM2Minor isoform of tropomyosinMinor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
3191YIL139CREV7Accessory subunit of DNA polymerase zetaAccessory subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev3p, Pol31p and Pol32p
3192YIL140WAXL2Integral plasma membrane proteinIntegral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
3193YIL141WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3194YIL142C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF CCT2/YIL142W
3195YIL142WCCT2Subunit beta of the cytosolic chaperonin Cct ring complexSubunit beta of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
3196YIL143CSSL2Component of RNA polymerase transcription factor TFIIH holoenzymeComponent of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to the cytosol in response to hypoxia; homolog of human ERCC3
3197YIL144WNDC80Component of the kinetochore-associated Ndc80 complexComponent of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation
3198YIL145CPAN6Pantothenate synthasePantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
3199YIL146CATG32Mitochondrial outer membrane protein required to initiate mitophagyMitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane; regulates mitophagy and ethanol production during alcoholic fermentation
3200YIL147CSLN1Transmembrane histidine phosphotransfer kinase and osmosensorTransmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
3201YIL148WRPL40AUbiquitin-ribosomal 60S subunit protein L40A fusion proteinUbiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
3202YIL149CMLP2Myosin-like protein associated with the nuclear envelopeMyosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication
3203YIL150CMCM10Essential chromatin-associated proteinEssential chromatin-associated protein; involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins; required to stabilize the catalytic subunit of DNA polymerase-alpha
3204YIL151CESL1hEST1A/B (SMG5/6)-like proteinProtein of unknown function; predicted to contain a PINc domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication
3205YIL152WYIL152WPutative protein of unknown functionPutative protein of unknown function
3206YIL153WRRD1Peptidyl-prolyl cis/trans-isomerasePeptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
3207YIL154CIMP2'Transcriptional activator involved in maintenance of ion homeostasisTranscriptional activator involved in maintenance of ion homeostasis; also involved in protection against DNA damage caused by bleomycin and other oxidants; contains a C-terminal leucine-rich repeat
3208YIL155CGUT2Mitochondrial glycerol-3-phosphate dehydrogenaseMitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner
3209YIL156WUBP7Ubiquitin-specific protease that cleaves ubiquitin-protein fusionsUbiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP7 has a paralog, UBP11, that arose from the whole genome duplication
3210YIL156W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF COA1/YIL157C
3211YIL156W-BMCO8Putative protein of unknown functionPutative protein of unknown function; originally identified based on homology to Ashbya gossypii and other related yeasts
3212YIL157CCOA1Mitochondrial inner membrane proteinMitochondrial inner membrane protein; required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
3213YIL158WAIM20Protein of unknown functionPutative protein of unknown function; overexpression causes cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress; AIM20 has a paralog, SKG1, that arose from the whole genome duplication
3214YIL159WBNR1ForminFormin; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1
3215YIL160CPOT13-ketoacyl-CoA thiolase with broad chain length specificity3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
3216YIL161WYIL161WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene
3217YIL162WSUC2InvertaseInvertase; sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
3218YIL163CYIL163CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data
3219YIL164CNIT1NitrilaseNitrilase; member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
3220YIL165CYIL165CPutative protein of unknown functionPutative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene
3221YIL166CSOA1Sulfonate and inorganic sulfur transporterPutative protein with similarity to allantoate permease; similar to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
3222YIL169CCSS1Protein of unknown function, secreted when constitutively expressedPutative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; YIL169C is a non-essential gene
3223YIL171W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF IMA3/YIL172C
3224YIL172CIMA3Alpha-glucosidaseAlpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima3p overexpression allows the ima1 null mutant to grow on isomaltose; lower activitiy and thermostability in vitro than Ima2p despite sequence difference of only 3 amino acids; cleaves alpha-1,3 linkage of nigerose and turanose, but not alpha-1,5 of leucrose; identical to IMA4
3225YIL173WVTH1Putative membrane glycoproteinPutative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
3226YIL176CPAU14Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau1p
3227YIL177CYIL177CPutative Y' element ATP-dependent helicasePutative Y' element ATP-dependent helicase
3228YIL177W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YIL177C
3229YIR001CSGN1Cytoplasmic RNA-binding proteinCytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
3230YIR002CMPH13'-5' DNA helicase involved in error-free bypass of DNA lesions3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA in error-free bypass pathway, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; similar to FANCM human Fanconi anemia complementation group protein that with MHF complex is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases
3231YIR003WAIM21Subunit of a complex that associates with actin filamentsProtein of unknown function; involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton
3232YIR004WDJP1Cytosolic J-domain-containing proteinCytosolic J-domain-containing protein; required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
3233YIR005WIST3Component of the U2 snRNPComponent of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
3234YIR006CPAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3pPart of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease
3235YIR007WEGH1Steryl-beta-glucosidase with broad specificity for aglyconesPutative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
3236YIR008CPRI1Subunit of DNA primaseSubunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
3237YIR009WMSL1U2B component of U2 snRNPU2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
3238YIR010WDSN1Essential component of the MIND kinetochore complexEssential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation of Dsn1p promotes interaction between outer and inner kinetochore proteins; N-terminal end interacts with monopolin subunit Csm1p and is essential for meiotic but not mitotic chromosome segregation; important for chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND); modified by sumoylation
3239YIR011CSTS1Protein required for localizing proteasomes to the nucleusProtein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation
3240YIR012WSQT1Specific chaperone for ribosomal protein Rpl10pProtein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
3241YIR013CGAT4Protein containing GATA family zinc finger motifsProtein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT3, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
3242YIR014WVLD1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
3243YIR015WRPR2Subunit of nuclear RNase PSubunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
3244YIR016WYIR016WPutative protein of unknown functionPutative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
3245YIR017CMET28bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complexbZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
3246YIR017W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3247YIR018C-AYIR018C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
3248YIR018WYAP5Basic leucine zipper (bZIP) iron-sensing transcription factorBasic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
3249YIR019CFLO11GPI-anchored cell surface glycoprotein (flocculin)GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain; a portion is cleaved and shed from cells, and free extracellular Flo11p contributes to the surface properties of cells
3250YIR020CYIR020CProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data
3251YIR020C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF MRS1
3252YIR020W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3253YIR021WMRS1Splicing proteinSplicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication
3254YIR021W-AYIR021W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
3255YIR022WSEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC)18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p
3256YIR023C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF DAL81/YIR023W
3257YIR023WDAL81Positive regulator of genes in multiple nitrogen degradation pathwaysPositive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
3258YIR024CINA22F1F0 ATP synthase peripheral stalk assembly factorF1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect
3259YIR025WMND2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
3260YIR026CYVH1Dual specificity protein phosphataseProtein phosphatase; involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
3261YIR027CDAL1AllantoinaseAllantoinase; converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
3262YIR028WDAL4Allantoin permeaseAllantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
3263YIR029WDAL2AllantoicaseAllantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
3264YIR030CDCG1Protein of unknown functionProtein of unknown function; expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain
3265YIR030W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF DAL7/YIR031C
3266YIR031CDAL7Malate synthaseMalate synthase; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA; recycles glyoxylate generated during allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
3267YIR032CDAL3Ureidoglycolate lyaseUreidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to ureidoglycolate hydrolase but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide
3268YIR033WMGA2ER membrane protein involved in regulation of OLE1 transcriptionER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication
3269YIR034CLYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming)Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity
3270YIR035CYIR035CPutative cytoplasmic short-chain dehydrogenase/reductasePutative cytoplasmic short-chain dehydrogenase/reductase
3271YIR036CIRC24Putative benzil reductasePutative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
3272YIR036W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF IRC24/YIR036C
3273YIR037WHYR1Thiol peroxidaseThiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication
3274YIR038CGTT1ER associated glutathione S-transferaseER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
3275YIR039CYPS6Putative GPI-anchored aspartic proteasePutative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance
3276YIR040CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3277YIR041WPAU15Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
3278YIR042CYIR042CPutative protein of unknown functionPutative protein of unknown function; YIR042C is a non-essential gene
3279YJL001WPRE3Beta 1 subunit of the 20S proteasomeBeta 1 subunit of the 20S proteasome; responsible for cleavage after acidic residues in peptides
3280YJL002COST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumenAlpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
3281YJL003WCOX16Mitochondrial inner membrane proteinMitochondrial inner membrane protein; required for assembly of cytochrome c oxidase
3282YJL004CSYS1Integral membrane protein of the GolgiIntegral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
3283YJL005WCYR1Adenylate cyclaseAdenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
3284YJL006CCTK2Beta subunit of C-terminal domain kinase I (CTDK-I)Beta subunit of C-terminal domain kinase I (CTDK-I); which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; relocalizes to the cytosol in response to hypoxia
3285YJL007CYJL007CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3286YJL008CCCT8Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
3287YJL009WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
3288YJL010CNOP9Essential subunit of U3-containing 90S preribosomeEssential subunit of U3-containing 90S preribosome; involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
3289YJL011CRPC17RNA polymerase III subunit C17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
3290YJL012CVTC4Vacuolar membrane polyphosphate polymeraseVacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress
3291YJL013CMAD3Subunit of spindle-assembly checkpoint complexSubunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
3292YJL014WCCT3Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; capable of binding Q/N rich proteins and mediating their folding
3293YJL015CDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
3294YJL016WTPH3Putative protein of unknown functionPutative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species
3295YJL019WMPS3Nuclear envelope proteinNuclear envelope protein; required for SPB insertion, initiation of SPB duplication and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology)
3296YJL020CBBC1Protein possibly involved in assembly of actin patchesProtein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
3297YJL020W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL020C/BBC1
3298YJL022WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
3299YJL023CPET130Protein required for respiratory growthProtein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3300YJL024CAPS3Small subunit of the clathrin-associated adaptor complex AP-3Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
3301YJL025WRRN7Component of the core factor (CF) rDNA transcription factor complexComponent of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
3302YJL026C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL026W/RNR2
3303YJL026WRNR2Ribonucleotide-diphosphate reductase (RNR), small subunitRibonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication
3304YJL027CYJL027CPutative protein of unknown functionPutative protein of unknown function
3305YJL028WYJL028WProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments
3306YJL029CVPS53Component of the GARP (Golgi-associated retrograde protein) complexComponent of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p,
3307YJL030WMAD2Component of the spindle-assembly checkpoint complexComponent of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
3308YJL031CBET4Alpha subunit of Type II geranylgeranyltransferaseAlpha subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
3309YJL032WDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
3310YJL033WHCA4DEAD box RNA helicaseDEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
3311YJL034WKAR2ATPase involved in protein import into the ERATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
3312YJL035CTAD2Subunit of tRNA-specific adenosine-34 deaminaseSubunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
3313YJL036WSNX4Sorting nexinSorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
3314YJL037WIRC18Protein involved in outer spore wall assemblyProtein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, LOH1/YJL038C, and the double mutant irc18 loh1 exhibits reduced dityrosine fluorescence relative to the single mutants; null mutant displays increased levels of spontaneous Rad52p foci
3315YJL038CLOH1Protein involved in outer spore wall assemblyProtein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; proposed role in maintenance of genome integrity; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to adjacent ORF, IRC18/YJL037W, and the double mutant irc18 loh1 exhibits reduced dityrosine fluorescence relative to the single mutants
3316YJL039CNUP192Essential subunit of inner ring of nuclear pore complex (NPC)Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
3317YJL041WNSP1FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p)
3318YJL042WMHP1Microtubule-associated protein involved in microtubule organizationMicrotubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
3319YJL043WYJL043WPutative protein of unknown functionPutative protein of unknown function; YJL043W is a non-essential gene
3320YJL044CGYP6GTPase-activating protein (GAP) for yeast Rab family member Ypt6pGTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport
3321YJL045WYJL045WMinor succinate dehydrogenase isozymeMinor succinate dehydrogenase isozyme; participates in oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner; YJL045W has a paralog, SDH1, that arose from the whole genome duplication
3322YJL046WAIM22Putative lipoate-protein ligasePutative lipoate-protein ligase; required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
3323YJL047CRTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complexCullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
3324YJL047C-AYJL047C-APutative protein of unknown functionPutative protein of unknown function
3325YJL048CUBX6UBX (ubiquitin regulatory X) domain-containing proteinUBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication
3326YJL049WCHM7Yeast homolog of human CHMP7, localizes to the endoplasmic reticulumPutative protein of unknown function; YJL049W is a non-essential gene
3327YJL050WMTR4ATP-dependent 3'-5' RNA helicase of the DExD/H familyATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
3328YJL051WIRC8Bud tip localized protein of unknown functionBud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
3329YJL052C-AYJL052C-APutative protein of unknown functionPutative protein of unknown function; identified based on comparison to related yeast species
3330YJL052WTDH1Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria
3331YJL053WPEP8Vacuolar protein component of the retromerVacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
3332YJL054WTIM54Component of the mitochondrial TIM22 complexComponent of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane
3333YJL055WYJL055WPutative protein of unknown functionPutative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA
3334YJL056CZAP1Zinc-regulated transcription factorZinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
3335YJL057CIKS1Protein kinase of unknown cellular roleProtein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
3336YJL058CBIT61Subunit of TORC2 membrane-associated complexSubunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
3337YJL059WYHC3Protein required for the ATP-dependent transport of arginineProtein required for the ATP-dependent transport of arginine; vacuolar membrane protein; involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis)
3338YJL060WBNA3Kynurenine aminotransferaseKynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
3339YJL061WNUP82Linker nucleoporin component of the nuclear pore complex (NPC)Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia
3340YJL062WLAS21Integral plasma membrane proteinIntegral plasma membrane protein; involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity
3341YJL062W-ACOA3Mitochondrial protein required for cytochrome c oxidase assemblyMitochondrial protein required for cytochrome c oxidase assembly; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; located in the mitochondrial inner membrane
3342YJL063CMRPL8Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
3343YJL064WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
3344YJL065CDLS1Subunit of ISW2/yCHRAC chromatin accessibility complexSubunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication
3345YJL066CMPM1Mitochondrial intermembrane space protein of unknown functionMitochondrial intermembrane space protein of unknown function
3346YJL067WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3347YJL068CYJL068CEsterase that can function as an S-formylglutathione hydrolaseEsterase that can function as an S-formylglutathione hydrolase; non-essential intracellular esterase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
3348YJL069CUTP18Small-subunit processome protein involved in pre-18S rRNA maturationSmall-subunit processome protein involved in pre-18S rRNA maturation; part of a subunit of the 90S preribosomal particle capable of interacting directly with the 5' ETS of the 35S pre-rRNA; contains WD40 repeats
3349YJL070CYJL070CPutative metallo-dependent hydrolase superfamily proteinPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
3350YJL071WARG2Acetylglutamate synthase (glutamate N-acetyltransferase)Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
3351YJL072CPSF2Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
3352YJL073WJEM1DnaJ-like chaperone required for nuclear membrane fusion during matingDnaJ-like chaperone required for nuclear membrane fusion during mating; localizes to the ER membrane; exhibits genetic interactions with KAR2
3353YJL074CSMC3Subunit of the multiprotein cohesin complexSubunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
3354YJL075CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate
3355YJL076WNET1Core subunit of the RENT complexCore subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
3356YJL077CICS3Protein with a role in copper homeostasisProtein with a role in processing of secretory proteins; possible role in vacuolar sorting, null mutants are hypersensitive to sortin2
3357YJL077W-AYJL077W-AProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data; completely overlaps the verified gene YJL077C/ICS3
3358YJL077W-BYJL077W-BPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3359YJL078CPRY3Cell wall-associated protein involved in export of acetylated sterolsCell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
3360YJL079CPRY1Sterol binding protein involved in the export of acetylated sterolsSterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication
3361YJL080CSCP160Essential RNA-binding G protein effector of mating response pathwayEssential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
3362YJL081CARP4Nuclear actin-related protein involved in chromatin remodelingNuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
3363YJL082WIML2Protein required for clearance of inclusion bodiesProtein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
3364YJL083WTAX4EH domain-containing proteinEH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
3365YJL084CALY2Alpha arrestinAlpha arrestin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication
3366YJL085WEXO70Subunit of the exocyst complexSubunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
3367YJL086CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
3368YJL087CTRL1tRNA ligasetRNA ligase; required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
3369YJL088WARG3Ornithine carbamoyltransferaseOrnithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline
3370YJL089WSIP4C6 zinc cluster transcriptional activatorC6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
3371YJL090CDPB11DNA replication initiation proteinDNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
3372YJL091CGWT1Protein involved in the inositol acylation of GlcN-PIProtein involved in the inositol acylation of GlcN-PI; the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) forms glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
3373YJL092WSRS2DNA helicase and DNA-dependent ATPaseDNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1
3374YJL093CTOK1Outward-rectifier potassium channel of the plasma membraneOutward-rectifier potassium channel of the plasma membrane; has two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin
3375YJL094CKHA1Putative K+/H+ antiporterPutative K+/H+ antiporter; has a probable role in intracellular cation homeostasis; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
3376YJL095WBCK1MAPKKK acting in the protein kinase C signaling pathwayMAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase
3377YJL096WMRPL49Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
3378YJL097WPHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membraneEssential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
3379YJL098WSAP185Protein that forms a complex with the Sit4p protein phosphataseProtein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
3380YJL099WCHS6Member of the ChAPs (Chs5p-Arf1p-binding proteins) familyMember of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
3381YJL100WLSB6Type II phosphatidylinositol 4-kinaseType II phosphatidylinositol 4-kinase; binds Las17p, a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
3382YJL101CGSH1Gamma glutamylcysteine synthetaseGamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
3383YJL102WMEF2Mitochondrial elongation factor involved in translational elongationMitochondrial elongation factor involved in translational elongation
3384YJL103CGSM1Putative zinc cluster protein of unknown functionPutative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis
3385YJL104WPAM16Subunit of the import motor (PAM complex)Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
3386YJL105WSET4Protein of unknown function, contains a SET domainProtein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
3387YJL106WIME2Serine/threonine protein kinase involved in activation of meiosisSerine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
3388YJL107CYJL107CPutative protein of unknown functionPutative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
3389YJL108CPRM10Pheromone-regulated proteinPheromone-regulated protein; proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation
3390YJL109CUTP10Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
3391YJL110CGZF3GATA zinc finger proteinGATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
3392YJL111WCCT7Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
3393YJL112WMDV1Peripheral protein of cytosolic face of mitochondrial outer membranePeripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
3394YJL113WYJL113WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
3395YJL114WYJL114WRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
3396YJL115WASF1Nucleosome assembly factorNucleosome assembly factor; involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition; relocalizes to the cytosol in response to hypoxia
3397YJL116CNCA3Protein involved in mitochondrion organizationProtein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication
3398YJL117WPHO86Endoplasmic reticulum (ER) resident proteinEndoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
3399YJL118WYJL118WProtein of unknown functionPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
3400YJL119CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3401YJL120WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
3402YJL121CRPE1D-ribulose-5-phosphate 3-epimeraseD-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
3403YJL122WALB1Shuttling pre-60S factorShuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
3404YJL123CMTC1Protein of unknown function that may interact with ribosomesProtein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
3405YJL124CLSM1Lsm (Like Sm) proteinLsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress
3406YJL125CGCD14Subunit of tRNA (1-methyladenosine) methyltransferaseSubunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
3407YJL126WNIT2Nit proteinNit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
3408YJL127CSPT10Histone H3 acetylase with a role in transcriptional regulationPutative histone acetylase with a role in transcriptional silencing; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
3409YJL127C-BMCO6Putative protein of unknown functionPutative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii
3410YJL127W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3411YJL128CPBS2MAP kinase kinase of the HOG signaling pathwayMAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
3412YJL129CTRK1Component of the Trk1p-Trk2p potassium transport systemComponent of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
3413YJL130CURA2Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylaseBifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
3414YJL131CAIM23Mitochondrial translation initiation factor 3 (IF3, mIF3)Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
3415YJL132WYJL132WPutative protein of unknown functionPutative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
3416YJL133C-ADPI8Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3417YJL133WMRS3Iron transporter, mediates Fe2+ transport across inner mito membraneIron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
3418YJL134WLCB3Long-chain base-1-phosphate phosphataseLong-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
3419YJL135WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
3420YJL136CRPS21BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
3421YJL136W-AYJL136W-APutative protein of unknown functionPutative protein of unknown function; identified by SAGE
3422YJL137CGLG2Glycogenin glucosyltransferaseGlycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
3423YJL138CTIF2Translation initiation factor eIF4ATranslation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
3424YJL139CYUR1Mannosyltransferase involved in protein N-glycosylationMannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
3425YJL140WRPB4RNA polymerase II subunit B32RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
3426YJL141CYAK1Serine-threonine protein kinaseSerine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose
3427YJL142CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci
3428YJL143WTIM17Essential component of the TIM23 complexEssential component of the TIM23 complex; with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core Translocase of the Inner Mitochondrial membrane (TIM23 complex)
3429YJL144WYJL144WCytoplasmic hydrophilin essential in desiccation-rehydration processCytoplasmic hydrophilin essential in desiccation-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
3430YJL145WSFH5Non-classical phosphatidylinositol transfer protein (PITP)Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress
3431YJL146WIDS2Protein involved in modulation of Ime2p activity during meiosisProtein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
3432YJL147CMRX5Protein that associates with mitochondrial ribosomeMitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene
3433YJL148WRPA34RNA polymerase I subunit A34.5RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p
3434YJL149WDAS1Putative SCF ubiquitin ligase F-box proteinPutative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
3435YJL150WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3436YJL151CSNA3Protein involved in efficient MVB sorting of proteins to the vacuoleProtein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
3437YJL152WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3438YJL153CINO1Inositol-3-phosphate synthaseInositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
3439YJL154CVPS35Endosomal subunit of membrane-associated retromer complexEndosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
3440YJL155CFBP26Fructose-2,6-bisphosphatase, required for glucose metabolismFructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
3441YJL156CSSY5Serine protease of SPS plasma membrane amino acid sensor systemSerine protease of SPS plasma membrane amino acid sensor system; contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p; other members are Ssy1p and Ptr3p
3442YJL156W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3443YJL157CFAR1CDK inhibitor and nuclear anchorCDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
3444YJL158CCIS3Mannose-containing glycoprotein constituent of the cell wallMannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
3445YJL159WHSP150O-mannosylated heat shock proteinO-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
3446YJL160CPIR5Putative protein of unknown functionPutative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication
3447YJL161WFMP33Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3448YJL162CJJJ2Protein of unknown functionProtein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein
3449YJL163CYJL163CPutative protein of unknown functionPutative protein of unknown function
3450YJL164CTPK1cAMP-dependent protein kinase catalytic subunitcAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
3451YJL165CHAL5Putative protein kinasePutative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
3452YJL166WQCR8Subunit 8 of ubiquinol cytochrome-c reductase (Complex III)Subunit 8 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
3453YJL167WERG20Farnesyl pyrophosphate synthetaseFarnesyl pyrophosphate synthetase; has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
3454YJL168CSET2Histone methyltransferase with a role in transcriptional elongationHistone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia
3455YJL169WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
3456YJL170CASG7Protein that regulates signaling from G protein beta subunit Ste4pProtein that regulates signaling from G protein beta subunit Ste4p; contributes to relocalization of Ste4p within the cell; specific to a-cells and induced by alpha-factor
3457YJL171CTOH1GPI-anchored cell wall protein of unknown functionGPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
3458YJL172WCPS1Vacuolar carboxypeptidase SVacuolar carboxypeptidase S; expression is induced under low-nitrogen conditions
3459YJL173CRFA3Subunit of heterotrimeric Replication Protein A (RPA)Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
3460YJL174WKRE9Glycoprotein involved in cell wall beta-glucan assemblyGlycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
3461YJL175WDubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor
3462YJL176CSWI3Subunit of the SWI/SNF chromatin remodeling complexSubunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
3463YJL177WRPL17BRibosomal 60S subunit protein L17BRibosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
3464YJL178CATG27Type I membrane protein involved in autophagy and the Cvt pathwayType I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site
3465YJL179WPFD1Subunit of heterohexameric prefoldinSubunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
3466YJL180CATP12Assembly factor for F1 sector of mitochondrial F1F0 ATP synthaseAssembly factor for the F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for the assembly of alpha and beta subunits into the F1 sector of the mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
3467YJL181WRBH1Putative protein of unknown functionPutative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication
3468YJL182CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W
3469YJL183WMNN11Subunit of a Golgi mannosyltransferase complexSubunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
3470YJL184WGON7Component of the EKC/KEOPS protein complexComponent of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; implicated in osmotic stress response; other complex members are Kae1p, Cgi121p, Pcc1p, and Bud32p
3471YJL185CATG36Pex3p interacting protein, required for pexophagyPex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene
3472YJL186WMNN5Alpha-1,2-mannosyltransferaseAlpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
3473YJL187CSWE1Protein kinase that regulates the G2/M transitionProtein kinase that regulates the G2/M transition; regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; phosphorylates conserved tyrosine residue in N-terminus of Hsp90 in cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; homolog of S. pombe Wee1p; potential Cdc28p substrate
3474YJL188CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
3475YJL189WRPL39Ribosomal 60S subunit protein L39Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
3476YJL190CRPS22AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication
3477YJL191WRPS14BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication
3478YJL192CSOP4ER-membrane proteinER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER
3479YJL193WYJL193WPutative protein of unknown functionPutative protein of unknown function; predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
3480YJL194WCDC6Essential ATP-binding protein required for DNA replicationEssential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
3481YJL195CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
3482YJL196CELO1Elongase I, medium-chain acyl elongaseElongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
3483YJL197C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL197C/UBP12
3484YJL197WUBP12Ubiquitin-specific proteaseUbiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm
3485YJL198WPHO90Low-affinity phosphate transporterLow-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
3486YJL199CMBB1Putative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies
3487YJL200CACO2Putative mitochondrial aconitase isozymePutative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
3488YJL201WECM25Non-essential protein of unknown functionNon-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p
3489YJL202CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
3490YJL203WPRP21Subunit of the SF3a splicing factor complexSubunit of the SF3a splicing factor complex; required for spliceosome assembly
3491YJL204CRCY1F-box protein involved in recycling endocytosed proteinsF-box protein involved in recycling endocytosed proteins; involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth; direct interaction with C-terminal cytoplasmic region of Drs2p plays an important role for Drs2p function in endocytic recycling pathway
3492YJL205CNCE101Protein of unknown functionProtein of unknown function; involved in secretion of proteins that lack classical secretory signal sequences
3493YJL206CYJL206CPutative protein of unknown functionPutative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment
3494YJL207CLAA1AP-1 accessory proteinAP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
3495YJL208CNUC1Major mitochondrial nucleaseMajor mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG
3496YJL209WCBP1Mitochondrial protein, regulator of COB mRNA stability and translationMitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
3497YJL210WPEX2RING-finger peroxin and E3 ubiquitin ligaseRING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
3498YJL211CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
3499YJL212COPT1Proton-coupled oligopeptide transporter of the plasma membraneProton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
3500YJL213WYJL213WProtein of unknown function that may interact with ribosomesProtein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
3501YJL214WHXT8Protein of unknown function with similarity to hexose transportersProtein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose
3502YJL215CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3503YJL216CIMA5Alpha-glucosidaseAlpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; most distant member of the IMA isomaltase family, but with similar catalytic properties as Ima1p and Ima2p; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose; can cleave alpha-1,3 linkage of nigerose and turanose and alpha-1,5 linkage of leucrose and is very sensitive to temperature in vitro
3504YJL217WREE1Cytoplasmic protein involved in the regulation of enolase (ENO1)Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
3505YJL218WYJL218WPutative acetyltransferasePutative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
3506YJL219WHXT9Putative hexose transporter that is nearly identical to Hxt11pPutative hexose transporter that is nearly identical to Hxt11p; has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p
3507YJL220WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL221C/FSP2
3508YJL221CIMA4Alpha-glucosidaseAlpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima4p overexpression allows the ima1 null mutant to grow on isomaltose; identical to IMA3
3509YJL222WVTH2Putative membrane glycoproteinPutative membrane glycoprotein; has strong similarity to Vth1p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
3510YJL222W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3511YJL222W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3512YJL223CPAU1Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis, negatively regulated by oxygen; repressed by heme; identical to Pau14p
3513YJL225CYJL225CPutative Y' element ATP-dependent helicasePutative Y' element ATP-dependent helicase
3514YJL225W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YJL225C
3515YJR001WAVT1Vacuolar transporterVacuolar transporter; imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
3516YJR002WMPP10Component of the SSU processome and 90S preribosomeComponent of the SSU processome and 90S preribosome; required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
3517YJR003CMRX12Protein that associates with mitochondrial ribosomePutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis
3518YJR004CSAG1Alpha-agglutinin of alpha-cellsAlpha-agglutinin of alpha-cells; binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
3519YJR005C-ALSO1Protein with a potential role in response to iron deprivationPutative protein of unknown function; transcription increases during treatment with 2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and copper, and may be regulated by Aft1p; originally identified as a syntenic homolog of an Ashbya gossypii gene; LSO1 has a paralog, LSO2, that arose from the whole genome duplication
3520YJR005WAPL1Beta-adaptinBeta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
3521YJR006WPOL31Subunit of DNA polymerase delta (polymerase III)Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia
3522YJR007WSUI2Alpha subunit of the translation initiation factor eIF2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
3523YJR008WMHO1Protein of unknown functionProtein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
3524YJR009CTDH2Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication
3525YJR010C-ASPC1Subunit of the signal peptidase complex (SPC)Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
3526YJR010WMET3ATP sulfurylaseATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism
3527YJR011CYJR011CPutative protein of unknown functionPutative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
3528YJR012CYJR012CEssential protein of unknown functionEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
3529YJR013WGPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase IGlycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I; involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
3530YJR014WTMA22Protein of unknown functionProtein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
3531YJR015WYJR015WPutative protein of unknown functionPutative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication
3532YJR016CILV3Dihydroxyacid dehydrataseDihydroxyacid dehydratase; catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
3533YJR017CESS1Peptidylprolyl-cis/trans-isomerase (PPIase)Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
3534YJR018WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3535YJR019CTES1Peroxisomal acyl-CoA thioesterasePeroxisomal acyl-CoA thioesterase; likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
3536YJR020WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3537YJR021CREC107Protein involved in early stages of meiotic recombinationProtein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
3538YJR022WLSM8Lsm (Like Sm) proteinLsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
3539YJR023CPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
3540YJR024CMDE15'-methylthioribulose-1-phosphate dehydratase5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
3541YJR025CBNA13-hydroxyanthranilic acid dioxygenase3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
3542YJR026WYJR026WRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
3543YJR027WYJR027WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
3544YJR028WYJR028WRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
3545YJR029WYJR029WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
3546YJR030CRBH2Putative protein of unknown functionPutative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; non-essential gene; YJR030C has a paralog, YJL181W, that arose from the whole genome duplication
3547YJR031CGEA1Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs)Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
3548YJR032WCPR7Peptidyl-prolyl cis-trans isomerase (cyclophilin)Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants
3549YJR033CRAV1Subunit of RAVE complex (Rav1p, Rav2p, Skp1p)Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
3550YJR034WPET191Protein required for assembly of cytochrome c oxidaseProtein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery
3551YJR035WRAD26Protein involved in transcription-coupled nucleotide excision repairProtein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
3552YJR036CHUL4Protein with similarity to hect domain E3 ubiquitin-protein ligasesProtein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex
3553YJR037WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces
3554YJR038CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3555YJR039WMLO127Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3556YJR040WGEF1Voltage-gated chloride channelVoltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
3557YJR041CURB2Protein required for normal metabolism of the rRNA primary transcriptProtein required for normal metabolism of the rRNA primary transcript; nucleolar protein; proposed to be involved in ribosome biogenesis
3558YJR042WNUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
3559YJR043CPOL32Third subunit of DNA polymerase deltaThird subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p
3560YJR044CVPS55Late endosomal protein involved in late endosome to vacuole transportLate endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
3561YJR045CSSC1Hsp70 family ATPaseHsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
3562YJR046WTAH11DNA replication licensing factorDNA replication licensing factor; required for pre-replication complex assembly
3563YJR047CANB1Translation elongation factor eIF-5ATranslation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication
3564YJR048WCYC1Cytochrome c, isoform 1Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; mutations in human homolog cause insulin-responsive hyperglycemia; CYC1 has a paralog, CYC7, that arose from the whole genome duplication
3565YJR049CUTR1ATP-NADH kinaseATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
3566YJR050WISY1Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
3567YJR051WOSM1Fumarate reductase, catalyzes the reduction of fumarate to succinateFumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication
3568YJR052WRAD7Nucleotide excision repair (NER) proteinNucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
3569YJR053WBFA1Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2pComponent of the GTPase-activating Bfa1p-Bub2p complex; involved in multiple cell cycle checkpoint pathways that control exit from mitosis; specifically required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p
3570YJR054WKCH1Potassium transporter that mediates K+ influxPotassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; localized to sites of polarized growth; member of a fungal-specific gene family; potential Cdc28p substrate; KCH1 has a paralog, PRM6, that arose from the whole genome duplication
3571YJR055WHIT1Protein involved in C/D snoRNP assemblyProtein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3
3572YJR056CYJR056CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
3573YJR057WCDC8Nucleoside monophosphate and nucleoside diphosphate kinaseThymidylate and uridylate kinase; functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
3574YJR058CAPS2Small subunit of the clathrin-associated adaptor complex AP-2Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
3575YJR059WPTK2Serine/threonine protein kinasePutative serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication
3576YJR060WCBF1Basic helix-loop-helix (bHLH) proteinBasic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress
3577YJR061WMNN14Putative protein of unknown functionPutative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication
3578YJR062CNTA1AmidaseAmidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation
3579YJR063WRPA12RNA polymerase I subunit A12.2RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
3580YJR064WCCT5Subunit of the cytosolic chaperonin Cct ring complexSubunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
3581YJR065CARP3Essential component of the Arp2/3 complexEssential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
3582YJR066WTOR1PIK-related protein kinase and rapamycin targetPIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
3583YJR067CYAE1Protein that forms a complex with Lto1p and Rli1pProtein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
3584YJR068WRFC2Subunit of heteropentameric Replication factor C (RF-C)Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
3585YJR069CHAM1Nucleoside triphosphate pyrophosphohydrolaseNucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimidines from deoxyribonucleoside triphosphate pools; functions together with YJL055W to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA without affecting uptake or incorporation of uracil into RNA; protein abundance increases in response to DNA replication stress
3586YJR070CLIA1Deoxyhypusine hydroxylaseDeoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
3587YJR071WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3588YJR072CNPA3Member of the conserved GPN-loop GTPase familyMember of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
3589YJR073COPI3Methylene-fatty-acyl-phospholipid synthaseMethylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
3590YJR074WMOG1Conserved nuclear protein that interacts with GTP-Gsp1pConserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
3591YJR075WHOC1Alpha-1,6-mannosyltransferaseAlpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
3592YJR076CCDC11Component of the septin ring that is required for cytokinesisComponent of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
3593YJR077CMIR1Mitochondrial phosphate carrierMitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
3594YJR078WBNA2Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenasePutative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase; required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp
3595YJR079WYJR079WPutative protein of unknown functionPutative protein of unknown function; mutation results in impaired mitochondrial respiration
3596YJR080CAIM24Protein with a role in determining mitochondrial architectureProtein with a role in determining mitochondrial architecture; inner membrane protein that interacts physically and genetically with the MICOS complex and is required for its integrity
3597YJR082CEAF6Subunit of the NuA4 acetyltransferase complexSubunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
3598YJR083CACF4Protein of unknown functionProtein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
3599YJR084WYJR084WProtein that forms a complex with Thp3pProtein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
3600YJR085CTMH11Protein of unknown functionProtein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
3601YJR086WSTE18G protein gamma subunitG protein gamma subunit; forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling
3602YJR087WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
3603YJR088CEMC2Member of conserved ER transmembrane complexMember of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
3604YJR089WBIR1Subunit of chromosomal passenger complex (CPC)Subunit of chromosomal passenger complex (CPC); CPC is comprised of Ipl1p-Sli15p-Bir1p-Nbl1p and regulates chromosome segregation; required for chromosome bi-orientation and for spindle assembly checkpoint activation upon reduced sister kinetochore tension; relative distribution to shortened microtubules increases upon DNA replication stress; sumoylated in an Mms21p-dependent manner; human survivin homolog
3605YJR090CGRR1F-box protein component of an SCF ubiquitin-ligase complexF-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification
3606YJR091CJSN1Member of the Puf family of RNA-binding proteinsMember of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication
3607YJR092WBUD4Anillin-like protein involved in bud-site selectionAnillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p
3608YJR093CFIP1Subunit of cleavage polyadenylation factor (CPF)Subunit of cleavage polyadenylation factor (CPF); interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
3609YJR094CIME1Master regulator of meiosis that is active only during meiotic eventsMaster regulator of meiosis that is active only during meiotic events; activates transcription of early meiotic genes through interaction with Ume6p; degraded by the 26S proteasome following phosphorylation by Ime2p; transcription is negatively regulated in cis by the IRT1 long noncoding antisense RNA
3610YJR094W-ARPL43BRibosomal 60S subunit protein L43BRibosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
3611YJR095WSFC1Mitochondrial succinate-fumarate transporterMitochondrial succinate-fumarate transporter; transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization
3612YJR096WYJR096WXylose and arabinose reductaseXylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
3613YJR097WJJJ3Protein of unknown functionProtein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
3614YJR098CYJR098CPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3615YJR099WYUH1Ubiquitin C-terminal hydrolaseUbiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
3616YJR100CAIM25Mitochondria protein of unknown functionPutative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss
3617YJR101WRSM26Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
3618YJR102CVPS25Component of the ESCRT-II complexComponent of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome
3619YJR103WURA8Minor CTP synthase isozyme (see also URA7)Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
3620YJR104CSOD1Cytosolic copper-zinc superoxide dismutaseCytosolic copper-zinc superoxide dismutase; detoxifies superoxide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p and enters the nucleus under oxidative stress to promote transcription of stress response genes; human ortholog implicated in ALS; abundance increases under DNA replication stress and during exposure to boric acid; localization of a fraction to the mitochondrial intermembrane space is modulated by the MICOS complex
3621YJR105WADO1Adenosine kinaseAdenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
3622YJR106WECM27Protein involved in calcium homeostasis and exit from quiescencePutative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p
3623YJR107WLIH1Putative lipasePutative lipase
3624YJR108WABM1Protein of unknown functionProtein of unknown function; required for normal microtubule organization
3625YJR109CCPA2Large subunit of carbamoyl phosphate synthetaseLarge subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor
3626YJR110WYMR1Phosphatidylinositol 3-phosphate (PI3P) phosphatasePhosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
3627YJR111CPXP2Peroxisomal matrix protein with naturally active promoterPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
3628YJR112WNNF1Essential component of the MIND kinetochore complexEssential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
3629YJR112W-AYJR112W-APutative protein of unknown functionPutative protein of unknown function; identified based on homology to Ashbya gossypii
3630YJR113CRSM7Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein
3631YJR114WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSM7/YJR113C
3632YJR115WYJR115WPutative protein of unknown functionPutative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
3633YJR116WTDA4Putative protein of unknown functionPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
3634YJR117WSTE24Highly conserved zinc metalloproteaseHighly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
3635YJR118CILM1Protein of unknown functionProtein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
3636YJR119CJHD2JmjC domain family histone demethylaseJmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing
3637YJR120WYJR120WProtein of unknown functionProtein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
3638YJR121WATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthaseBeta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
3639YJR122WIBA57Protein involved in incorporating iron-sulfur clusters into proteinsProtein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
3640YJR123WRPS5Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
3641YJR124CYJR124CPutative protein of unknown functionPutative protein of unknown function; expression induced under calcium shortage
3642YJR125CENT3Protein containing an N-terminal epsin-like domainProtein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p
3643YJR126CVPS70Protein of unknown function involved in vacuolar protein sortingProtein of unknown function involved in vacuolar protein sorting
3644YJR127CRSF2Zinc-finger proteinZinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
3645YJR128WYJR128WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2
3646YJR129CEFM3S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A
3647YJR130CSTR2Cystathionine gamma-synthase, converts cysteine into cystathionineCystathionine gamma-synthase, converts cysteine into cystathionine; STR2 has a paralog, YML082W, that arose from the whole genome duplication
3648YJR131WMNS1Alpha-1,2-mannosidaseAlpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
3649YJR132WNMD5KaryopherinKaryopherin; a carrier protein involved in nuclear import of proteins; importin beta homolog
3650YJR133WXPT1Xanthine-guanine phosphoribosyl transferaseXanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine
3651YJR134CSGM1Protein of unknown functionProtein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
3652YJR135CMCM22Outer kinetochore protein and component of the Ctf3 subcomplexOuter kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
3653YJR135W-ATIM8Mitochondrial intermembrane space proteinMitochondrial intermembrane space protein; forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome
3654YJR136CTTI2Subunit of the ASTRA complex, involved in chromatin remodelingSubunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti2p; may interact with Rsm23p; GFP-fusion protein localizes to the cytoplasm
3655YJR137CMET5Sulfite reductase beta subunitSulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine
3656YJR138WIML1GTPase-activating protein (GAP) subunit of the Iml1p/SEACIT complexGTPase-activating protein (GAP), subunit of SEA and Iml1p complexes; SEA (Seh1-associated) complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; localized to either pre-autophagosomal structures (PAS) or non-PAS structures during NNS-induced autophagy;
3657YJR139CHOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase)Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase); dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
3658YJR140CHIR3Subunit of the HIR complexSubunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
3659YJR140W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene HIR3; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3660YJR141WIPA1Protein implicated in pre-mRNA processing and proteasomal degradationEssential protein of unknown function
3661YJR142WYJR142W8-oxo-dGTP diphosphatase of the Nudix hydrolase family8-oxo-dGTP diphosphatase of the Nudix hydrolase family; converts diphosphates of damaged forms of thiamin to monophosphates; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition
3662YJR143CPMT4Protein O-mannosyltransferaseProtein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals
3663YJR144WMGM101Protein with a role in mitochondrial DNA recombinational repairProtein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance
3664YJR145CRPS4AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
3665YJR146WYJR146WProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
3666YJR147WHMS2Protein with similarity to heat shock transcription factorsProtein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
3667YJR148WBAT2Cytosolic branched-chain amino acid (BCAA) aminotransferaseCytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication
3668YJR149WYJR149WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
3669YJR150CDAN1Cell wall mannoproteinCell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
3670YJR151CDAN4Cell wall mannoproteinCell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
3671YJR151W-AYJR151W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis
3672YJR152WDAL5Allantoate permeaseAllantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
3673YJR153WPGU1Endo-polygalacturonaseEndo-polygalacturonase; pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
3674YJR154WYJR154WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
3675YJR155WAAD10Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
3676YJR156CTHI11Protein involved in synthesis of the thiamine precursor HMPProtein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
3677YJR157WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3678YJR158WHXT16Putative transmembrane polyol transporterProtein of unknown function with similarity to hexose transporters; expression is repressed by high levels of glucose
3679YJR159WSOR1Sorbitol dehydrogenaseSorbitol dehydrogenase; expression is induced in the presence of sorbitol or xylose
3680YJR160CMPH3Alpha-glucoside permeaseAlpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication
3681YJR161CCOS5Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
3682YJR162CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3683YKL001CMET14Adenylylsulfate kinaseAdenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism
3684YKL002WDID4Class E Vps protein of the ESCRT-III complexClass E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
3685YKL003CMRP17Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
3686YKL004WAUR1Phosphatidylinositol:ceramide phosphoinositol transferasePhosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase
3687YKL005CBYE1Negative regulator of transcription elongationNegative regulator of transcription elongation; contains a TFIIS-like domain that associates with chromatin and a PHD domain that interacts with H3K4me3; multicopy suppressor of temperature-sensitive ess1 mutations, binds RNA polymerase II large subunit
3688YKL006C-ASFT1Intra-Golgi v-SNAREIntra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment
3689YKL006WRPL14ARibosomal 60S subunit protein L14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
3690YKL007WCAP1Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p)Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
3691YKL008CLAC1Ceramide synthase componentCeramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
3692YKL009WMRT4Protein involved in mRNA turnover and ribosome assemblyProtein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus
3693YKL010CUFD4Ubiquitin-protein ligase (E3)Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
3694YKL011CCCE1Mitochondrial cruciform cutting endonucleaseMitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication
3695YKL012WPRP40U1 snRNP protein involved in splicingU1 snRNP protein involved in splicing; interacts with the branchpoint-binding protein during the formation of the second commitment complex
3696YKL013CARC19Subunit of the ARP2/3 complexSubunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
3697YKL014CURB1Protein required for the normal accumulation of 25S and 5.8S rRNAsProtein required for the normal accumulation of 25S and 5.8S rRNAs; nucleolar protein; associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
3698YKL015WPUT3Transcriptional activatorTranscriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
3699YKL016CATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthaseSubunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
3700YKL017CHCS1Hexameric DNA polymerase alpha-associated DNA helicase AHexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
3701YKL018C-AMCO12Putative protein of unknown functionPutative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
3702YKL018WSWD2Subunit of the COMPASS (Set1C) histone H3K4 methyltransferase complexSubunit of the COMPASS (Set1C) histone H3K4 methyltransferase complex; required for Set1C stability and optimal activity; COMPASS methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
3703YKL019WRAM2Alpha subunit of farnesyltransferase and geranylgeranyltransferase-IAlpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (RAM2-RAM1 heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (RAM2-CDC43 heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor
3704YKL020CSPT23ER membrane protein involved in regulation of OLE1 transcriptionER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
3705YKL021CMAK11Protein involved in an early step of 60S ribosomal subunit biogenesisProtein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
3706YKL022CCDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C)Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
3707YKL023C-AMIN9Putative protein of unknown functionPutative protein of unknown function
3708YKL023WYKL023WPutative protein of unknown functionPutative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
3709YKL024CURA6Uridylate kinaseUridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
3710YKL025CPAN3Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complexEssential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes
3711YKL026CGPX1Phospholipid hydroperoxide glutathione peroxidasePhospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; GPX1 has a paralog, HYR1, that arose from the whole genome duplication
3712YKL027WTCD2tRNA threonylcarbamoyladenosine dehydratasetRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication
3713YKL028WTFA1TFIIE large subunitTFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
3714YKL029CMAE1Mitochondrial malic enzymeMitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
3715YKL030WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
3716YKL031WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species
3717YKL032CIXR1Transcriptional repressor that regulates hypoxic genes during normoxiaTranscriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication
3718YKL033WTTI1Subunit of the ASTRA complex, involved in chromatin remodelingSubunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
3719YKL033W-AYKL033W-APutative protein of unknown functionPutative protein of unknown function; similar to uncharacterized proteins from other fungi
3720YKL034WTUL1Subunit of the DSC ubiquitin ligase complexSubunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
3721YKL035WUGP1UDP-glucose pyrophosphorylase (UGPase)UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
3722YKL036CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
3723YKL037WAIM26Protein of unknown functionPutative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
3724YKL038WRGT1Glucose-responsive transcription factorGlucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
3725YKL039WPTM1Protein of unknown functionProtein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
3726YKL040CNFU1Protein involved in Fe-S cluster transfer to mitochondrial clientsProtein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria
3727YKL041WVPS24One of four subunits of the ESCRT-III complexOne of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
3728YKL042WSPC42Central plaque component of spindle pole body (SPB)Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
3729YKL043WPHD1Transcriptional activator that enhances pseudohyphal growthTranscriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
3730YKL044WMMO1Protein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data
3731YKL045WPRI2Subunit of DNA primaseSubunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
3732YKL046CDCW1Putative mannosidasePutative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
3733YKL047WANR2Protein of unknown functionPutative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated
3734YKL048CELM1Serine/threonine protein kinaseSerine/threonine protein kinase that regulates cellular morphogenesis; septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
3735YKL049CCSE4Centromeric histone H3-like proteinCentromere protein that resembles histone H3; associated with promoters, accessible chromatin and RNA polymerase II-bound regions; phosphorylated Cse4p associates with centromeres; required for proper kinetochore function; levels regulated by E3 ubiquitin ligase Psh1p; phosphorylation of Cse4p may destabilize defective kinetochores to promote bi-orientation; ubiquitination of N terminus regulates Cse4p proteolysis for faithful chromosome segregation; human CENP-A homolog
3736YKL050CYKL050CProtein of unknown functionProtein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p; YKL050C has a paralog, EIS1, that arose from the whole genome duplication
3737YKL051WSFK1Plasma membrane protein that may act to generate normal levels of PI4PPlasma membrane protein that may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane
3738YKL052CASK1Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress
3739YKL053C-AMDM35Mitochondrial intermembrane space proteinMitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
3740YKL053WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
3741YKL054CDEF1RNAPII degradation factorRNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
3742YKL055COAR1Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductaseMitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p
3743YKL056CTMA19Protein that associates with ribosomesProtein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
3744YKL057CNUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
3745YKL058WTOA2TFIIA small subunitTFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa1p, for ribosomal protein gene transcription in vivo; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
3746YKL059CMPE1Essential conserved subunit of CPF cleavage and polyadenylation factorEssential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif; relocalizes to the cytosol in response to hypoxia
3747YKL060CFBA1Fructose 1,6-bisphosphate aldolaseFructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo
3748YKL061WBLI1Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome
3749YKL062WMSN4Stress-responsive transcriptional activatorStress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift
3750YKL063CYKL063CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
3751YKL064WMNR2Vacuolar membrane protein required for magnesium homeostasisVacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
3752YKL065CYET1Endoplasmic reticulum transmembrane proteinEndoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication
3753YKL065W-ADPC7Putative protein of unknown functionPutative protein of unknown function
3754YKL066WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
3755YKL067WYNK1Nucleoside diphosphate kinaseNucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
3756YKL068WNUP100FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication
3757YKL068W-AYKL068W-APutative protein of unknown functionPutative protein of unknown function; identified by homology to Ashbya gossypii
3758YKL069WYKL069WMethionine-R-sulfoxide reductaseMethionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress
3759YKL070WYKL070WPutative protein of unknown functionPutative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3760YKL071WYKL071WPutative protein of unknown functionPutative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
3761YKL072WSTB6Protein that binds Sin3p in a two-hybrid assayProtein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication
3762YKL073WLHS1Molecular chaperone of the endoplasmic reticulum lumenMolecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
3763YKL074CMUD2Protein involved in early pre-mRNA splicingProtein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
3764YKL075CYKL075CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
3765YKL076CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
3766YKL077WPSG1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
3767YKL078WDHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicasePredominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
3768YKL079WSMY1Kinesin-like myosin passenger-proteinKinesin-like myosin passenger-protein; interacts with Myo2p; controls actin cable structure and dynamics; proposed to be involved in exocytosis
3769YKL080WVMA5Subunit C of the V1 peripheral membrane domain of V-ATPaseSubunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
3770YKL081WTEF4Gamma subunit of translational elongation factor eEF1BGamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
3771YKL082CRRP14Essential protein, constituent of 66S pre-ribosomal particlesEssential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
3772YKL083WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
3773YKL084WHOT13Zinc-binding mitochondrial intermembrane space (IMS) proteinZinc-binding mitochondrial intermembrane space (IMS) protein; involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
3774YKL085WMDH1Mitochondrial malate dehydrogenaseMitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
3775YKL086WSRX1SulfiredoxinSulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
3776YKL087CCYT2Cytochrome c1 heme lyaseCytochrome c1 heme lyase; involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
3777YKL088WCAB3Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesisSubunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC
3778YKL089WMIF2Protein required for structural integrity of elongating spindlesProtein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
3779YKL090WCUE2Protein of unknown functionProtein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
3780YKL091CYKL091CPutative phosphatidylinositol/phosphatidylcholine transfer proteinPutative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication
3781YKL092CBUD2GTPase activating factor for Rsr1p/Bud1pGTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types
3782YKL093WMBR1Protein involved in mitochondrial functions and stress responseProtein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; MBR1 has a paralog, ISF1, that arose from the whole genome duplication
3783YKL094WYJU3Monoglyceride lipase (MGL)Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
3784YKL095WYJU2Essential protein required for pre-mRNA splicingEssential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
3785YKL096C-BYKL096C-BPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3786YKL096WCWP1Cell wall mannoprotein that localizes to birth scars of daughter cellsCell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance
3787YKL096W-ACWP2Covalently linked cell wall mannoproteinCovalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
3788YKL097CYKL097CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species
3789YKL098WMTC2Protein of unknown functionProtein of unknown function; mtc2 is synthetically sick with cdc13-1
3790YKL099CUTP11Subunit of U3-containing Small Subunit (SSU) processome complexSubunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
3791YKL100CYPF1Intramembrane aspartyl protease of the perinuclear ER membranePutative protein of unknown function; has similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene
3792YKL100W-APutative protein of unknown function; identified by expression profiling and mass spectrometry
3793YKL101WHSL1Nim1p-related protein kinaseNim1p-related protein kinase; regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p
3794YKL102CYKL102CPutative protein of unknown functionDubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
3795YKL103CAPE1Vacuolar aminopeptidase yscIVacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress
3796YKL104CGFA1Glutamine-fructose-6-phosphate amidotransferaseGlutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
3797YKL105CSEG2Eisosome componentEisosome component; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication
3798YKL106C-AYKL106C-APutative protein of unknown functionPutative protein of unknown function; identified by homology to uncharacterized proteins in other fungi
3799YKL106WAAT1Mitochondrial aspartate aminotransferaseMitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
3800YKL107WYKL107WPutative short-chain dehydrogenase/reductasePutative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein
3801YKL108WSLD2Single-stranded DNA origin-binding and annealing proteinSingle-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
3802YKL109WHAP4Transcription factorTranscription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift
3803YKL110CKTI12Protein that plays a role in modification of tRNA wobble nucleosidesProtein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
3804YKL111CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
3805YKL112WABF1DNA binding protein with possible chromatin-reorganizing activityDNA binding protein with possible chromatin-reorganizing activity; involved in transcriptional activation, gene silencing, and DNA replication and repair
3806YKL113CRAD275' to 3' exonuclease, 5' flap endonuclease5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia
3807YKL114CAPN1Major apurinic/apyrimidinic endonucleaseMajor apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity
3808YKL115CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRR1
3809YKL116CPRR1Serine/threonine protein kinaseSerine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor
3810YKL117WSBA1Co-chaperone that binds and regulates Hsp90 family chaperonesCo-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
3811YKL118WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPH2
3812YKL119CVPH2Integral membrane protein required for V-ATPase functionIntegral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
3813YKL120WOAC1Mitochondrial inner membrane transporterMitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
3814YKL121WDGR2Protein of unknown functionProtein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication
3815YKL122CSRP21Subunit of the signal recognition particle (SRP)Subunit of the signal recognition particle (SRP); SRP functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
3816YKL123WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SSH4
3817YKL124WSSH4Specificity factor required for Rsp5p-dependent ubiquitinationSpecificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases
3818YKL125WRRN3Protein required for transcription of rDNA by RNA polymerase IProtein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
3819YKL126WYPK1S/T protein kinaseSerine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
3820YKL127WPGM1Phosphoglucomutase, minor isoformPhosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication
3821YKL128CPMU1Putative phosphomutasePutative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
3822YKL129CMYO3One of two type I myosinsOne of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication
3823YKL130CSHE2RNA-binding protein that binds specific mRNAs and interacts with She3pRNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER
3824YKL131WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3825YKL132CRMA1Putative dihydrofolate synthetasePutative dihydrofolate synthetase; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; RMA1 has a paralog, FOL3, that arose from the whole genome duplication
3826YKL133CRCI50Putative protein of unknown functionPutative protein of unknown function; not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype); YKL133C has a paralog, MGR3, that arose from the whole genome duplication
3827YKL134COCT1Mitochondrial intermediate peptidaseMitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis
3828YKL135CAPL2Beta-adaptin subunit of the clathrin-associated protein (AP-1) complexBeta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
3829YKL136WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
3830YKL137WCMC1Copper-binding protein of the mitochondrial intermembrane spaceCopper-binding protein of the mitochondrial intermembrane space; evolutionarily conserved; may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif
3831YKL138CMRPL31Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
3832YKL138C-AHSK3Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
3833YKL139WCTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I)Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
3834YKL140WTGL1Steryl ester hydrolaseSteryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
3835YKL141WSDH3Subunit of succinate dehydrogenase and of TIM22 translocaseSubunit of succinate dehydrogenase and of TIM22 translocase; functions as cytochrome b subunit of succinate dehydrogenase, which couples oxidation of succinate to transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex; SDH3 has a paralog, SHH3, that arose from the whole genome duplication
3836YKL142WMRP8Protein of unknown functionProtein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
3837YKL143WLTV1Subunit of the EGO/GSE complexComponent of the GSE complex; GSE is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature
3838YKL144CRPC25RNA polymerase III subunit C25RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
3839YKL145WRPT1ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
3840YKL145W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified essential gene RPT1; identified by expression profiling and mass spectrometry
3841YKL146WAVT3Vacuolar transporterVacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
3842YKL147CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3
3843YKL148CSDH1Flavoprotein subunit of succinate dehydrogenaseFlavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; SDH1 has a paralog, YJL045W, that arose from the whole genome duplication
3844YKL149CDBR1RNA lariat debranching enzymeRNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies
3845YKL150WMCR1Mitochondrial NADH-cytochrome b5 reductaseMitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis
3846YKL151CNNR2Widely-conserved NADHX dehydrataseNADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
3847YKL152CGPM1Tetrameric phosphoglycerate mutaseTetrameric phosphoglycerate mutase; mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
3848YKL153WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
3849YKL154WSRP102Signal recognition particle (SRP) receptor beta subunitSignal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
3850YKL155CRSM22Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
3851YKL156C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene RPS27A; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3852YKL156WRPS27AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
3853YKL157WAPE2Aminopeptidase yscIIAminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication
3854YKL159CRCN1Protein involved in calcineurin regulation during calcium signalingProtein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
3855YKL160WELF1Transcription elongation factor with a conserved zinc finger domainTranscription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
3856YKL161CKDX1Protein kinaseProtein kinase; implicated in Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p; KDX1 has a paralog, SLT2, that arose from the whole genome duplication
3857YKL162CYKL162CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
3858YKL162C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3859YKL163WPIR3O-glycosylated covalently-bound cell wall proteinO-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication
3860YKL164CPIR1O-glycosylated protein required for cell wall stabilityO-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
3861YKL165CMCD4Protein involved in GPI anchor synthesisProtein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol
3862YKL165C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3863YKL166CTPK3cAMP-dependent protein kinase catalytic subunitcAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
3864YKL167CMRP49Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
3865YKL168CKKQ8Putative serine/threonine protein kinase with unknown cellular rolePutative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
3866YKL169CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
3867YKL170WMRPL38Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
3868YKL171WNNK1Protein kinaseProtein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
3869YKL172WEBP2Required for 25S rRNA maturation and 60S ribosomal subunit assemblyRequired for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
3870YKL173WSNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosomeGTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
3871YKL174CTPO5Protein involved in excretion of putrescine and spermidineProtein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
3872YKL175WZRT3Vacuolar membrane zinc transporterVacuolar membrane zinc transporter; transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency
3873YKL176CLST4Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2pProtein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
3874YKL177WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3
3875YKL178CSTE3Receptor for a factor pheromoneReceptor for a factor pheromone; couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR
3876YKL179CCOY1Golgi membrane protein with similarity to mammalian CASPGolgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
3877YKL180WRPL17ARibosomal 60S subunit protein L17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
3878YKL181WPRS15-phospho-ribosyl-1(alpha)-pyrophosphate synthetase5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; plays a key role in cell wall integrity (CWI) pathway; one of five related enzymes, which are active as heteromultimeric complexes; missense mutations in human homolog PRPS1 are associated with neuropathic Arts syndrome and Charcot-Marie Tooth (CMTX5) disease
3879YKL182WFAS1Beta subunit of fatty acid synthetaseBeta subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
3880YKL183C-AYKL183C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
3881YKL183WLOT5Protein of unknown functionProtein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
3882YKL184WSPE1Ornithine decarboxylaseOrnithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
3883YKL185WASH1Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
3884YKL186CMTR2mRNA transport regulatormRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
3885YKL187CFAT3Protein required for fatty acid uptakeProtein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication
3886YKL188CPXA2Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1pSubunit of a heterodimeric peroxisomal ABC transport complex; required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; complex also includes Pxa1p
3887YKL189WHYM1Component of the RAM signaling networkComponent of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
3888YKL190WCNB1Calcineurin BCalcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress
3889YKL191WDPH2Protein required for synthesis of diphthamideProtein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
3890YKL192CACP1Mitochondrial matrix acyl carrier proteinMitochondrial matrix acyl carrier protein; involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
3891YKL193CSDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7pRegulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
3892YKL194CMST1Mitochondrial threonyl-tRNA synthetaseMitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro
3893YKL195WMIA40Import and assembly protein in mitochondrial intermembrane spaceMitochondrial oxidoreductase; involved in mitochondrial intermembrane space import; component of MIA pathway which mediates import and oxidative folding of substrates including small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p via a chaperone-like activity; forms a dimer that binds iron-sulfur cluster in vitro
3894YKL196CYKT6Vesicle membrane protein (v-SNARE) with acyltransferase activityVesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
3895YKL197CPEX1AAA-peroxinAAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis
3896YKL198CPTK1Putative serine/threonine protein kinasePutative serine/threonine protein kinase; regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein; PTK1 has a paralog, PTK2, that arose from the whole genome duplication
3897YKL201CMNN4Putative positive regulator of mannosylphosphate transferase Mnn6pPutative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; coding sequence contains length polymorphisms in different strains; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
3898YKL202WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3899YKL203CTOR2PIK-related protein kinase and rapamycin targetPIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
3900YKL204WEAP1eIF4E-associated protein, competes with eIF4G for binding to eIF4EeIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
3901YKL205WLOS1Nuclear pore proteinNuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
3902YKL206CADD66Protein involved in 20S proteasome assemblyProtein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
3903YKL207WEMC3Member of conserved ER transmembrane complexMember of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
3904YKL208WCBT1Protein involved in 5' RNA end processingProtein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
3905YKL209CSTE6Plasma membrane ATP-binding cassette (ABC) transporterPlasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells
3906YKL210WUBA1Ubiquitin activating enzyme (E1)Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
3907YKL211CTRP3Indole-3-glycerol-phosphate synthaseIndole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
3908YKL212WSAC1Phosphatidylinositol phosphate (PtdInsP) phosphatasePhosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
3909YKL213CDOA1WD-repeat protein involved in ubiquitin-mediated protein degradationWD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; facilitates N-terminus-dependent proteolysis of centromeric histone H3 (Cse4p) for faithful chromosome segregation; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
3910YKL214CYRA2Member of the REF (RNA and export factor binding proteins) familyMember of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
3911YKL215COXP15-oxoprolinase5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
3912YKL216WURA1Dihydroorotate dehydrogenaseDihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
3913YKL217WJEN1Monocarboxylate/proton symporter of the plasma membraneMonocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
3914YKL218CSRY13-hydroxyaspartate dehydratase3-hydroxyaspartate dehydratase; deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity
3915YKL219WCOS9Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
3916YKL220CFRE2Ferric reductase and cupric reductaseFerric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
3917YKL221WMCH2Protein with similarity to mammalian monocarboxylate permeasesProtein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
3918YKL222CYKL222CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
3919YKL223WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3920YKL224CPAU16Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
3921YKL225WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3922YKR001CVPS1Dynamin-like GTPase required for vacuolar sortingDynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
3923YKR002WPAP1Poly(A) polymerasePoly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
3924YKR003WOSH6Member of an oxysterol-binding protein familyMember of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication
3925YKR004CECM9Non-essential protein of unknown functionNon-essential protein of unknown function
3926YKR005CYKR005CPutative protein of unknown functionPutative protein of unknown function
3927YKR006CMRPL13Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
3928YKR007WMEH1Subunit of the EGO/GSE complexComponent of the EGO and GSE complexes; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
3929YKR008WRSC4Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
3930YKR009CFOX23-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase; multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway
3931YKR010CTOF2Protein required for rDNA silencing and mitotic rDNA condensationProtein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
3932YKR011CYKR011CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
3933YKR012CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2
3934YKR013WPRY2Sterol binding protein involved in the export of acetylated sterolsSterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
3935YKR014CYPT52Endosomal Rab family GTPaseEndosomal Rab family GTPase; required for vacuolar protein sorting, endocytosis and multivesicular body (MVB) biogenesis and sorting; required for localization of the CORVET complex to endosomes; involved in autophagy and ionic stress tolerance; similar to Vps21p and Ypt53p; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress
3936YKR015CYKR015CPutative protein of unknown functionPutative protein of unknown function
3937YKR016WMIC60Component of the MICOS complexComponent of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic60p is also involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; ortholog of mammalian mitofilin
3938YKR017CHEL1RING finger ubiquitin ligase (E3)RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)
3939YKR018CYKR018CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
3940YKR019CIRS4EH domain-containing proteinEH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
3941YKR020WVPS51Component of the GARP (Golgi-associated retrograde protein) complexComponent of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p,
3942YKR021WALY1Alpha arrestin, substrate of calcineurinAlpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
3943YKR022CNTR2Essential protein that forms a dimer with Ntr1pEssential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
3944YKR023WYKR023WPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3945YKR024CDBP7Putative ATP-dependent RNA helicase of the DEAD-box familyPutative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions
3946YKR025WRPC37RNA polymerase III subunit C37RNA polymerase III subunit C37
3947YKR026CGCN3Alpha subunit of translation initiation factor eIF2BAlpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions
3948YKR027WBCH2Member of the ChAPs (Chs5p-Arf1p-binding proteins) familyMember of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
3949YKR028WSAP190Protein that forms a complex with the Sit4p protein phosphataseProtein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
3950YKR029CSET3Defining member of the SET3 histone deacetylase complexDefining member of the SET3 histone deacetylase complex; which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication
3951YKR030WGMH1Golgi membrane protein of unknown functionGolgi membrane protein of unknown function; interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting
3952YKR031CSPO14Phospholipase DPhospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
3953YKR032WYKR032WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3954YKR033CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80
3955YKR034WDAL80Negative regulator of genes in multiple nitrogen degradation pathwaysNegative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
3956YKR035CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
3957YKR035W-ADID2Class E protein of the vacuolar protein-sorting (Vps) pathwayClass E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
3958YKR036CCAF4WD40 repeat-containing protein associated with the CCR4-NOT complexWD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
3959YKR037CSPC34Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
3960YKR038CKAE1Highly conserved ATPase of HSP70/DnaK familyHighly conserved ATPase of HSP70/DnaK family; essential functional component of the EKC/KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription
3961YKR039WGAP1General amino acid permeaseGeneral amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
3962YKR040CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
3963YKR041WYKR041WProtein of unknown functionProtein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
3964YKR042WUTH1Mitochondrial inner membrane proteinMitochondrial inner membrane protein; role in mitophagy is disputed; implicated in cell wall biogenesis, the oxidative stress response, life span during starvation, and cell death; SUN family member; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
3965YKR043CSHB17Sedoheptulose bisphosphatase involved in riboneogenesisSedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
3966YKR044WUIP5Protein of unknown function that interacts with Ulp1pProtein of unknown function that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates
3967YKR045CYKR045CPutative protein of unknown functionPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
3968YKR046CPLN1Protein of unknown function that localizes to lipid particlesProtein of unknown function that localizes to lipid particles; localization suggests a role in lipid metabolism; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
3969YKR047WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
3970YKR048CNAP1Histone chaperoneHistone chaperone; involved in histone exchange by removing and replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; involved in the transport of H2A and H2B histones to the nucleus; required for the regulation of microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
3971YKR049CFMP46Putative redox protein containing a thioredoxin foldPutative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3972YKR050WTRK2Component of the Trk1p-Trk2p potassium transport systemComponent of the Trk1p-Trk2p potassium transport system; contributes to K(+) supply and maintenance of plasma-membrane potential; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
3973YKR051WYKR051WPutative protein of unknown functionPutative protein of unknown function
3974YKR052CMRS4Iron transporter of the mitochondrial carrier familyIron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
3975YKR053CYSR3Dihydrosphingosine 1-phosphate phosphataseDihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication
3976YKR054CDYN1Cytoplasmic heavy chain dyneinCytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p
3977YKR055WRHO4Non-essential small GTPaseNon-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi
3978YKR056WTRM2tRNA methyltransferasetRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
3979YKR057WRPS21AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
3980YKR058WGLG1Glycogenin glucosyltransferaseGlycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
3981YKR059WTIF1Translation initiation factor eIF4ATranslation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication
3982YKR060WUTP30Putative subunit of U3-containing 90S preribosome complexPutative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit
3983YKR061WKTR2Mannosyltransferase involved in N-linked protein glycosylationMannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
3984YKR062WTFA2TFIIE small subunitTFIIE small subunit; involved in RNA polymerase II transcription initiation
3985YKR063CLAS1Endonuclease involved in pre-rRNA processing at both ends of ITS2Protein required for pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability
3986YKR064WOAF3Putative transcriptional repressor with Zn(2)-Cys(6) fingerPutative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
3987YKR065CPAM17Constituent of the TIM23 complexConstituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
3988YKR066CCCP1Mitochondrial cytochrome-c peroxidaseMitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress
3989YKR067WGPT2Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferaseGlycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
3990YKR068CBET3Core component of transport protein particle (TRAPP) complexes I-IIICore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles
3991YKR069WMET1S-adenosyl-L-methionine uroporphyrinogen III transmethylaseS-adenosyl-L-methionine uroporphyrinogen III transmethylase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
3992YKR070WYKR070WPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
3993YKR071CDRE2Component of the cytosolic Fe-S protein assembly (CIA) machineryComponent of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
3994YKR072CSIS2Negative regulatory subunit of protein phosphatase 1 (Ppz1p)Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication
3995YKR073CYKR073CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
3996YKR074WAIM29Protein of unknown functionPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
3997YKR075CYKR075CProtein of unknown functionProtein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication
3998YKR075W-ADubious open reading frame unlikely to encode a protein; completely overlaps the uncharacterized gene YKR075C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
3999YKR076WECM4S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR)Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
4000YKR077WMSA2Putative transcriptional activatorPutative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication
4001YKR078WYKR078WCytoplasmic protein of unknown functionCytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication
4002YKR079CTRZ1tRNA 3'-end processing endonuclease tRNase ZtRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
4003YKR080WMTD1NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenaseNAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
4004YKR081CRPF2Essential protein involved in rRNA maturation and ribosomal assemblyEssential protein involved in rRNA maturation and ribosomal assembly; involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
4005YKR082WNUP133Subunit of Nup84p subcomplex of nuclear pore complex (NPC)Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133
4006YKR083CDAD2Essential subunit of the Dam1 complex (aka DASH complex)Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
4007YKR084CHBS1GTPase with similarity to translation release factorsGTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication
4008YKR085CMRPL20Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
4009YKR086WPRP16DEAH-box RNA helicase involved in second catalytic step of splicing aDEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa
4010YKR087COMA1Metalloendopeptidase of the mitochondrial inner membraneMetalloendopeptidase of the mitochondrial inner membrane; important for adaptive responses to various homeostatic insults and preservation of normal mitochondrial function under damage-eliciting conditions; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes
4011YKR088CTVP38Integral membrane proteinIntegral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
4012YKR089CTGL4Multifunctional lipase/hydrolase/phospholipaseMultifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication
4013YKR090WPXL1Protein that localizes to sites of polarized growthProtein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
4014YKR091WSRL3GTB motif (G1/S transcription factor binding) containing proteinGTB motif (G1/S transcription factor binding) containing protein; binds SBF-regulated promoters in hydroxyurea-treated cells; when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate; SRL3 has a paralog, WHI5, that arose from the whole genome duplication
4015YKR092CSRP40Nucleolar serine-rich proteinNucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
4016YKR093WPTR2Integral membrane peptide transporterIntegral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
4017YKR094CRPL40BUbiquitin-ribosomal 60S subunit protein L40B fusion proteinUbiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
4018YKR095WMLP1Myosin-like protein associated with the nuclear envelopeMyosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
4019YKR095W-APCC1Component of the EKC/KEOPS protein complexComponent of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; other complex members are Kae1p, Gon7p, Bud32p, and Cgi121p
4020YKR096WESL2hEST1A/B (SMG5/6)-like proteinProtein of unknown function; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain; ESL2 has a paralog, ESL1, that arose from the whole genome duplication
4021YKR097WPCK1Phosphoenolpyruvate carboxykinasePhosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
4022YKR098CUBP11Ubiquitin-specific proteaseUbiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication
4023YKR099WBAS1Myb-related transcription factorMyb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes
4024YKR100CSKG1Transmembrane protein with a role in cell wall polymer compositionTransmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication
4025YKR101WSIR1Protein involved in silencing at mating-type loci HML and HMRProtein involved in silencing at mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin
4026YKR102WFLO10Member of the FLO family of cell wall flocculation proteinsMember of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
4027YKR103WNFT1Putative transporter of the MRP subfamilyPutative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
4028YKR104WYKR104WPutative transporter of the MRP subfamilyPutative transporter of the MRP subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
4029YKR105CVBA5Plasma membrane protein of the Major Facilitator Superfamily (MFS)Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication
4030YKR106WGEX2Proton:glutathione antiporterProton:glutathione antiporter; localized to the vacuolar and plasma membranes; expressed at a very low level; potential role in resistance to oxidative stress and modulation of the PKA pathway; GEX2 has a paralog, GEX1, that arose from a segmental duplication
4031YLL001WDNM1Dynamin-related GTPase involved in mitochondrial organizationDynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance
4032YLL002WRTT109Histone acetyltransferaseHistone acetyltransferase; critical for cell survival in the presence of DNA damage during S phase; prevents hyper-amplification of rDNA; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p
4033YLL003WSFI1Centrin (Cdc31p)-binding protein required for SPB duplicationCentrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C
4034YLL004WORC3Subunit of the origin recognition complex (ORC)Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
4035YLL005CSPO75Meiosis-specific protein of unknown functionMeiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
4036YLL006WMMM1ER integral membrane protein, ERMES complex subunitER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
4037YLL006W-AYLL006W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
4038YLL007CLMO1Homolog of mammalian ELMO (Engulfment and celL MOtility)Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene
4039YLL008WDRS1Nucleolar DEAD-box protein required for ribosome assembly and functionNucleolar DEAD-box protein required for ribosome assembly and function; including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
4040YLL009CCOX17Copper metallochaperone that transfers copper to Sco1p and Cox11pCopper metallochaperone that transfers copper to Sco1p and Cox11p; eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
4041YLL010CPSR1Plasma membrane associated protein phosphatasePlasma membrane associated protein phosphatase; involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR1 has a paralog, PSR2, that arose from the whole genome duplication
4042YLL011WSOF1Protein required for biogenesis of 40S (small) ribosomal subunitProtein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene
4043YLL012WYEH1Steryl ester hydrolaseSteryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication
4044YLL013CPUF3Protein of the mitochondrial outer surfaceProtein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
4045YLL014WEMC6Member of conserved ER transmembrane complexMember of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
4046YLL015WBPT1ABC type transmembrane transporter of MRP/CFTR familyABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p
4047YLL018CDPS1Aspartyl-tRNA synthetase, primarily cytoplasmicAspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation; shares five highly conserved amino acids with human that when mutated cause leukoencephalopathy characterized by hypomyelination with brain stem and spinal cord involvement and leg spasticity (HBSL)
4048YLL018C-ACOX19Protein required for cytochrome c oxidase assemblyProtein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
4049YLL019CKNS1Protein kinase involved in negative regulation of PolIII transcriptionProtein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
4050YLL019W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene KNS1
4051YLL020CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
4052YLL021WSPA2Component of the polarisomeComponent of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication
4053YLL022CHIF1Non-essential component of the HAT-B histone acetyltransferase complexNon-essential component of the HAT-B histone acetyltransferase complex; localized to the nucleus; has a role in telomeric silencing; other members are Hat1p and Hat2p
4054YLL023CPOM33Transmembrane nucleoporinTransmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
4055YLL024CSSA2HSP70 family ATP-binding proteinATP-binding protein; involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
4056YLL025WPAU17Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene
4057YLL026WHSP104DisaggregaseDisaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation
4058YLL027WISA1Protein required for maturation of mitochondrial [4Fe-4S] proteinsProtein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins
4059YLL028WTPO1Polyamine transporter of the major facilitator superfamilyPolyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane
4060YLL029WFRA1Protein involved in negative regulation of iron regulon transcriptionProtein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
4061YLL030CRRT7Putative protein of unknown functionProtein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
4062YLL031CGPI13ER membrane localized phosphoryltransferaseER membrane localized phosphoryltransferase; adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
4063YLL032CYLL032CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
4064YLL033WIRC19Protein of unknown functionPutative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
4065YLL034CRIX7Putative ATPase of the AAA familyPutative ATPase of the AAA family; required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
4066YLL035WGRC3Polynucleotide kinase present on rDNAPolynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated
4067YLL036CPRP19Splicing factor associated with the spliceosomeSplicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia
4068YLL037WDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
4069YLL038CENT4Protein of unknown functionProtein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
4070YLL039CUBI4UbiquitinUbiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress
4071YLL040CVPS13Protein involved in prospore membrane morphogenesisProtein involved in prospore membrane morphogenesis; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulatoin, and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
4072YLL041CSDH2Iron-sulfur protein subunit of succinate dehydrogenaseIron-sulfur protein subunit of succinate dehydrogenase; the complex couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; other members are Sdh1p, Sdh3p, and Sdh4p
4073YLL042CATG10Conserved E2-like conjugating enzymeConserved E2-like conjugating enzyme; mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
4074YLL043WFPS1Aquaglyceroporin, plasma membrane channelAquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; role in mediating passive diffusion of glycerol is key factor in maintenance of redox balance; member of major intrinsic protein (MIP) family; phosphorylated by Hog1p MAPK under acetate stress; deletion improves xylose fermentation
4075YLL044WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant
4076YLL045CRPL8BRibosomal 60S subunit protein L8BRibosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
4077YLL046CRNP1Ribonucleoprotein that contains two RNA recognition motifs (RRM)Ribonucleoprotein that contains two RNA recognition motifs (RRM); RNP1 has a paralog, SBP1, that arose from the whole genome duplication
4078YLL047WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
4079YLL048CYBT1Transporter of the ATP-binding cassette (ABC) familyTransporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication
4080YLL049WLDB18Component of the dynactin complexComponent of the dynactin complex; dynactin is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
4081YLL050CCOF1Cofilin, involved in pH-dependent actin filament depolarizationCofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
4082YLL051CFRE6Putative ferric reductase with similarity to Fre2pPutative ferric reductase with similarity to Fre2p; expression induced by low iron levels
4083YLL052CAQY2Water channel that mediates water transport across cell membranesWater channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
4084YLL053CYLL053CPutative proteinPutative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
4085YLL054CYLL054CPutative protein of unknown function with similarity to Pip2pPutative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
4086YLL055WYCT1High-affinity cysteine-specific transporterHigh-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
4087YLL056CYLL056CPutative protein of unknown functionPutative protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin
4088YLL057CJLP1Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenaseFe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase; involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation
4089YLL058WYLL058WPutative protein of unknown function with similarity to Str2pPutative protein of unknown function with similarity to Str2p; Str2p is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene
4090YLL059CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4091YLL060CGTT2Glutathione S-transferase capable of homodimerizationGlutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
4092YLL061WMMP1High-affinity S-methylmethionine permeaseHigh-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
4093YLL062CMHT1S-methylmethionine-homocysteine methyltransferaseS-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
4094YLL063CAYT1AcetyltransferaseAcetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis
4095YLL064CPAU18Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau6p
4096YLL065WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps chromosome XII telomeric element core sequences
4097YLL066CYLL066CPutative Y' element ATP-dependent helicasePutative Y' element ATP-dependent helicase; YLL066C is not an essential gene
4098YLL066W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YLL066C
4099YLL066W-BYLL066W-BPutative protein of unknown functionPutative protein of unknown function; overexpression causes a cell cycle delay or arrest
4100YLL067CYLL067CPutative Y' element ATP-dependent helicasePutative Y' element ATP-dependent helicase
4101YLL067W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps YLL067C
4102YLR001CYLR001CPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
4103YLR002CNOC3Subunit of a nuclear complex with Noc2p and pre-replicative complexesSubunit of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
4104YLR003CCMS1Putative subunit of the 90S preribosome processome complexPutative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
4105YLR004CTHI73Putative plasma membrane permeasePutative plasma membrane permease; proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
4106YLR005WSSL1Subunit of the core form of RNA polymerase transcription factor TFIIHSubunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p
4107YLR006CSSK1Cytoplasmic phosphorelay intermediate osmosensor and regulatorCytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
4108YLR007WNSE1Component of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
4109YLR008CPAM18Subunit of the import motor (PAM complex)Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
4110YLR009WRLP24Essential protein required for ribosomal large subunit biogenesisEssential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
4111YLR010CTEN1Protein that regulates telomeric lengthProtein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p; similar to human Ten1 which is critical for the telomeric function of the CST (Cdc13p-Stn1p-Ten1p) complex
4112YLR011WLOT6FMN-dependent NAD(P)H:quinone reductaseFMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
4113YLR012CYLR012CPutative protein of unknown functionPutative protein of unknown function; YLR012C is not an essential gene
4114YLR013WGAT3Protein containing GATA family zinc finger motifsProtein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT4, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
4115YLR014CPPR1Zinc finger transcription factorZinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
4116YLR015WBRE2Subunit of COMPASS (Set1C) complexSubunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
4117YLR016CPML1Subunit of the RES complexSubunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
4118YLR017WMEU1Methylthioadenosine phosphorylase (MTAP)Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
4119YLR018CPOM34Subunit of the transmembrane ring of the nuclear pore complex (NPC)Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
4120YLR019WPSR2Plasma membrane phosphatasePlasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR2 has a paralog, PSR1, that arose from the whole genome duplication
4121YLR020CYEH2Steryl ester hydrolaseSteryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication
4122YLR021WIRC25Component of a heterodimeric Poc4p-Irc25p chaperoneComponent of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
4123YLR022CSDO1Guanine nucleotide exchange factor (GEF) for Ria1pGuanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
4124YLR023CIZH3Membrane protein involved in zinc ion homeostasisMembrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
4125YLR024CUBR2Cytoplasmic ubiquitin-protein ligase (E3)Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
4126YLR025WSNF7One of four subunits of the ESCRT-III complexOne of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III
4127YLR026CSED5cis-Golgi t-SNARE syntaxincis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins
4128YLR027CAAT2Cytosolic aspartate aminotransferase involved in nitrogen metabolismCytosolic aspartate aminotransferase involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
4129YLR028CADE16Enzyme of 'de novo' purine biosynthesisEnzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
4130YLR029CRPL15ARibosomal 60S subunit protein L15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
4131YLR030WYLR030WPutative protein of unknown functionPutative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W
4132YLR031WYLR031WPutative protein of unknown functionPutative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W; YLR031W has a paralog, YMR124W, that arose from the whole genome duplication
4133YLR032WRAD5DNA helicase/Ubiquitin ligaseDNA helicase/Ubiquitin ligase; involved in error-free branch of DNA damage tolerance (DDT) pathway; proposed to promote replication fork regression during postreplication repair by template switching; stimulates synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells
4134YLR033WRSC58Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
4135YLR034CSMF3Putative divalent metal ion transporter involved in iron homeostasisPutative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
4136YLR035CMLH2Protein involved in mismatch repair and meiotic recombinationProtein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p
4137YLR035C-AYLR035C-ARetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); YLR035C-A is part of a mutant retrotransposon
4138YLR036CYLR036CPutative protein predicted to have transmembrane domainsPutative protein predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein
4139YLR037CPAU23Cell wall mannoproteinCell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
4140YLR038CCOX12Subunit VIb of cytochrome c oxidaseSubunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
4141YLR039CRIC1Protein involved in retrograde transport to the cis-Golgi networkProtein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
4142YLR040CAFB1MATalpha-specific a-factor blockerMATalpha-specific a-factor blocker; contributes to mating efficiency under certain conditions; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W
4143YLR041WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C
4144YLR042CYLR042CCell wall protein of unknown functionCell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLL042C is not an essential gene
4145YLR043CTRX1Cytoplasmic thioredoxin isoenzymeCytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication
4146YLR044CPDC1Major of three pyruvate decarboxylase isozymesMajor of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo
4147YLR045CSTU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 familyMicrotubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
4148YLR046CYLR046CPutative membrane proteinPutative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YLR046C has a paralog, RTA1, that arose from the whole genome duplication
4149YLR047CFRE8Protein with sequence similarity to iron/copper reductasesProtein with sequence similarity to iron/copper reductases; involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
4150YLR048WRPS0BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
4151YLR049CMLO50Putative protein of unknown functionPutative protein of unknown function
4152YLR050CYLR050CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene
4153YLR051CFCF2Nucleolar protein involved in the early steps of 35S rRNA processingNucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon; essential gene
4154YLR052WIES3Subunit of the INO80 chromatin remodeling complexSubunit of the INO80 chromatin remodeling complex
4155YLR053CYLR053CPutative protein of unknown functionPutative protein of unknown function
4156YLR054COSW2Protein of unknown function reputedly involved in spore wall assemblyProtein of unknown function reputedly involved in spore wall assembly
4157YLR055CSPT8Subunit of the SAGA transcriptional regulatory complexSubunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters
4158YLR056WERG3C-5 sterol desaturaseC-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase
4159YLR057WMNL2Putative mannosidase involved in ER-associated protein degradationPutative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
4160YLR058CSHM2Cytosolic serine hydroxymethyltransferaseCytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
4161YLR059CREX23'-5' RNA exonuclease3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
4162YLR060WFRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetaseBeta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
4163YLR061WRPL22ARibosomal 60S subunit protein L22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
4164YLR062CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
4165YLR063WBMT6Methyltransferase required for m3U2843 methylation of the 25S rRNAMethyltransferase required for m3U2843 methylation of the 25S rRNA; S-adenosylmethionine-dependent; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
4166YLR064WPER33Protein that localizes to the endoplasmic reticulumProtein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
4167YLR065CSND2Protein involved in SRP-independent targeting of substrates to the ERProtein proposed to be involved in vacuolar functions; putative role in secretory protein quality control; mutant shows defect in CPY processing; YLR065C is not an essential gene
4168YLR066WSPC3Subunit of signal peptidase complexSubunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p
4169YLR067CPET309Specific translational activator for the COX1 mRNASpecific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs)
4170YLR068WFYV7Essential protein required for maturation of 18S rRNAEssential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
4171YLR069CMEF1Mitochondrial elongation factor involved in translational elongationMitochondrial elongation factor involved in translational elongation
4172YLR070CXYL2Xylitol dehydrogenaseXylitol dehydrogenase; converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
4173YLR071CRGR1Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
4174YLR072WLAM6Sterol transporter that transfers sterols between membranesProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; not an esssential gene; YLR072W has a paralog, YFL042C, that arose from the whole genome duplication
4175YLR073CRFU1Protein that inhibits Doa4p deubiquitinating activityProtein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
4176YLR074CBUD20C2H2-type zinc finger protein required for ribosome assemblyC2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
4177YLR075WRPL10Ribosomal 60S subunit protein L10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
4178YLR076CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
4179YLR077WFMP25Protein required for assembly of respiratory complex IIIProtein required for assembly of respiratory complex III; mitochondrial inner membrane protein; required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
4180YLR078CBOS1v-SNARE (vesicle specific SNAP receptor)v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
4181YLR079WSIC1Cyclin-dependent kinase inhibitor (CKI)Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
4182YLR080WEMP46Integral membrane component of ER-derived COPII-coated vesiclesIntegral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication
4183YLR081WGAL2Galactose permeaseGalactose permease; required for utilization of galactose; also able to transport glucose
4184YLR082CSRL2Protein of unknown functionProtein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
4185YLR083CEMP70Protein with a role in cellular adhesion and filamentous growthProtein with a role in cellular adhesion and filamentous growth; also endosome-to-vacuole sorting; similar to Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments; EMP70 has a paralog, TMN2, that arose from the whole genome duplication
4186YLR084CRAX2N-glycosylated proteinN-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
4187YLR085CARP6Actin-related protein that binds nucleosomesActin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
4188YLR086WSMC4Subunit of the condensin complexSubunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
4189YLR087CCSF1Protein required for fermentation at low temperatureProtein required for fermentation at low temperature; plays a role in the maturation of secretory proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
4190YLR088WGAA1Subunit of the GPI:protein transamidase complexSubunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER
4191YLR089CALT1Alanine transaminase (glutamic pyruvic transaminase)Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive
4192YLR090WXDJ1Chaperone with a role in facilitating mitochondrial protein importChaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
4193YLR091WGEP5Protein of unknown functionProtein of unknown function; required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
4194YLR092WSUL2High affinity sulfate permeaseHigh affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
4195YLR093CNYV1v-SNARE component of the vacuolar SNARE complexv-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
4196YLR094CGIS3Protein of unknown functionProtein of unknown function
4197YLR095CIOC2Subunit of the Isw1b complexSubunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p
4198YLR096WKIN2Serine/threonine protein kinase involved in regulation of exocytosisSerine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN2 has a paralog, KIN1, that arose from the whole genome duplication
4199YLR097CHRT3Putative SCF-ubiquitin ligase F-box proteinPutative SCF-ubiquitin ligase F-box protein; based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
4200YLR098CCHA4DNA binding transcriptional activatorDNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
4201YLR099CICT1Lysophosphatidic acid acyltransferaseLysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication
4202YLR099W-AMIM2Mitochondrial protein required for outer membrane protein importMitochondrial protein required for outer membrane protein import; involved in import of the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and Fzo1p; component of a large protein complex in the outer membrane that includes Mim1p; not essential in W303 strain background
4203YLR100WERG273-keto sterol reductase3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
4204YLR101CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
4205YLR102CAPC9Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
4206YLR103CCDC45DNA replication initiation factorDNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
4207YLR104WLCL2Putative protein of unknown functionPutative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
4208YLR105CSEN2Subunit of the tRNA splicing endonucleaseSubunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
4209YLR106CMDN1Huge dynein-related AAA-type ATPase (midasin)Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
4210YLR107WREX3RNA exonucleaseRNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
4211YLR108CYLR108CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
4212YLR109WAHP1Thiol-specific peroxiredoxinThiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
4213YLR110CCCW12Cell wall mannoproteinCell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
4214YLR111WYLR111WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4215YLR112WYLR112WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4216YLR113WHOG1Mitogen-activated protein kinase involved in osmoregulationMitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII in osmotic shock; activates CDC28 by stimulating antisense RNA transcription; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; with Mrc1p defines novel S-phase checkpoint that prevent conflicts between DNA replication and transcription; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
4217YLR114CAVL9Conserved protein involved in exocytic transport from the GolgiConserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
4218YLR115WCFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF)Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
4219YLR116WMSL5Component of commitment complexComponent of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
4220YLR117CCLF1Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
4221YLR118CTML25Acyl-protein thioesterase responsible for depalmitoylation of Gpa1pAcyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
4222YLR119WSRN2Component of the ESCRT-I complexComponent of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
4223YLR120CYPS1Aspartic proteaseAspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; YPS1 has a paralog, MKC7, that arose from the whole genome duplication
4224YLR120W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR121C
4225YLR121CYPS3Aspartic proteaseAspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
4226YLR122CYLR122CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C
4227YLR123CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif
4228YLR124WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4229YLR125WYLR125WPutative protein of unknown functionPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
4230YLR126CYLR126CPutative glutamine amidotransferasePutative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
4231YLR127CAPC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
4232YLR128WDCN1Scaffold-type E3 ligaseScaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
4233YLR129WDIP2Nucleolar proteinNucleolar protein; specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
4234YLR130CZRT2Low-affinity zinc transporter of the plasma membraneLow-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
4235YLR131CACE2Transcription factor required for septum destruction after cytokinesisTranscription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
4236YLR132CUSB1Putative poly(U)-specific 3'-to-5' RNA exonucleasePutative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
4237YLR133WCKI1Choline kinaseCholine kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
4238YLR134WPDC5Minor isoform of pyruvate decarboxylaseMinor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
4239YLR135WSLX4Endonuclease involved in processing DNAEndonuclease involved in processing DNA; acts during recombination and repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
4240YLR136CTIS11mRNA-binding protein expressed during iron starvationmRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication
4241YLR137WRKM5Protein lysine methyltransferaseProtein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
4242YLR138WNHA1Na+/H+ antiporterNa+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
4243YLR139CSLS1Mitochondrial membrane proteinMitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
4244YLR140WDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
4245YLR141WRRN5Protein involved in transcription of rDNA by RNA polymerase IProtein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
4246YLR142WPUT1Proline oxidaseProline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
4247YLR143WDPH6Diphthamide synthetaseDiphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
4248YLR144CACF2Intracellular beta-1,3-endoglucanaseIntracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress
4249YLR145WRMP1Subunit of RNase MRPSubunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
4250YLR146CSPE4Spermine synthaseSpermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
4251YLR146W-AYLR146W-APutative protein of unknown functionPutative protein of unknown function
4252YLR147CSMD3Core Sm protein Sm D3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
4253YLR148WPEP3Component of CORVET membrane tethering complexComponent of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
4254YLR149CYLR149CProtein of unknown functionProtein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
4255YLR149C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4256YLR150WSTM1Protein required for optimal translation under nutrient stressProtein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
4257YLR151CPCD18-oxo-dGTP diphosphatase8-oxo-dGTP diphosphatase; prevents spontaneous mutagenesis via sanitization of oxidized purine nucleoside triphosphates; can also act as peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member; similar E. coli MutT and human, rat and mouse MTH1
4258YLR152CYLR152CPutative protein of unknown functionPutative protein of unknown function; YLR152C is not an essential gene
4259YLR153CACS2Acetyl-coA synthetase isoformAcetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
4260YLR154CRNH203Ribonuclease H2 subunitRibonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome
4261YLR154C-GYLR154C-GPutative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2
4262YLR154C-HYLR154C-HPutative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-2; YLR154C-H has a paralog, YLR157C-C, that arose from a segmental duplication
4263YLR154W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 25S rRNA gene on the opposite strand
4264YLR154W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 25S rRNA gene on the opposite strand
4265YLR154W-CTAR1Protein potentially involved in regulation of respiratory metabolismProtein potentially involved in regulation of respiratory metabolism; located in the mitochondria; interacts genetically with RPO41 and physically with Coq5p; encoded within the 25S rRNA gene on the opposite strand
4266YLR154W-EDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 35S rRNA gene on the opposite strand
4267YLR154W-FDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 35S rRNA gene on the opposite strand
4268YLR155CASP3-1Cell-wall L-asparaginase II involved in asparagine catabolismCell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-1 has a paralog, ASP3-3, that arose from a segmental duplication
4269YLR156C-AYLR156C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-3; YLR156C-A has a paralog, YLR159C-A, that arose from a segmental duplication
4270YLR156WYLR156WPutative protein of unknown functionPutative protein of unknown function; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis; YLR156W has a paralog, YLR159W, that arose from a segmental duplication
4271YLR157CASP3-2Cell-wall L-asparaginase II involved in asparagine catabolismCell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-2 has a paralog, ASP3-4, that arose from a segmental duplication
4272YLR157C-AYLR157C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4273YLR157C-BYLR157C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4274YLR157C-CYLR157C-CPutative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-4; YLR157C-C has a paralog, YLR154C-H, that arose from a segmental duplication
4275YLR157W-DYLR157W-DPutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YLR157W-D has a paralog, YLR161W, that arose from a segmental duplication
4276YLR157W-EYLR157W-EPutative protein of unknown function identified by gene-trappingPutative protein of unknown function identified by gene-trapping; microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element
4277YLR158CASP3-3Cell-wall L-asparaginase II involved in asparagine catabolismCell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication
4278YLR159C-AYLR159C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-5; YLR159C-A has a paralog, YLR156C-A, that arose from a segmental duplication
4279YLR159WYLR159WPutative protein of unknown functionPutative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR159W has a paralog, YLR156W, that arose from a segmental duplication
4280YLR160CASP3-4Cell-wall L-asparaginase II involved in asparagine catabolismCell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-4 has a paralog, ASP3-2, that arose from a segmental duplication
4281YLR161WYLR161WPutative protein of unknown functionPutative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR161W has a paralog, YLR157W-D, that arose from a segmental duplication
4282YLR162WYLR162WProtein of unknown functionPutative protein of unknown function; overexpression confers resistance to the antimicrobial peptide MiAMP1 and causes growth arrest, apoptosis, and increased sensitivity to cobalt chloride
4283YLR162W-ARRT15Putative protein of unknown functionPutative protein of unknown function; identified by fungal homology comparisons and RT-PCR; identified in a screen for mutants with decreased levels of rDNA transcription
4284YLR163CMAS1Beta subunit of the mitochondrial processing protease (MPP)Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
4285YLR163W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4286YLR164WSHH4Putative alternate subunit of succinate dehydrogenase (SDH)Putative alternate subunit of succinate dehydrogenase (SDH); mitochondrial inner membrane protein; genetic interaction with SDH4 suggests that Shh4p can function as a functional SDH subunit; a fraction copurifies with SDH subunit Sdh3p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Shh4p has greater similarity to human SDHD (subunit D of SDH, implicated in paraganglioma) than does its paralog Sdh4p
4287YLR165CPUS5Pseudouridine synthasePseudouridine synthase; catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
4288YLR166CSEC10Essential 100kDa subunit of the exocyst complexEssential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
4289YLR167WRPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitinFusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
4290YLR168CUPS2Mitochondrial intermembrane space proteinMitochondrial intermembrane space protein; involved in phospholipid metabolism; has role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI; UPS2 has a paralog, UPS3, that arose from the whole genome duplication
4291YLR169WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4292YLR170CAPS1Small subunit of the clathrin-associated adaptor complex AP-1Small subunit of the clathrin-associated adaptor complex AP-1; AP-1 is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
4293YLR171WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4294YLR172CDPH5Methyltransferase required for synthesis of diphthamideMethyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
4295YLR173WYLR173WPutative protein of unknown functionPutative protein of unknown function
4296YLR174WIDP2Cytosolic NADP-specific isocitrate dehydrogenaseCytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication
4297YLR175WCBF5Pseudouridine synthase catalytic subunit of box H/ACA snoRNPsPseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on large and small rRNAs, on snRNA U2, and on some mRNAs; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs
4298YLR176CRFX1Major transcriptional repressor of DNA-damage-regulated genesMajor transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
4299YLR177WYLR177WPutative protein of unknown functionPutative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
4300YLR178CTFS1Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p)Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
4301YLR179CYLR179CProtein of unknown function with similarity to Tfs1pProtein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
4302YLR180WSAM1S-adenosylmethionine synthetaseS-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
4303YLR181CVTA1Multivesicular body (MVB) proteinMultivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
4304YLR182WSWI6Transcription cofactorTranscription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
4305YLR183CTOS4Putative transcription factor, contains Forkhead Associated domainPutative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase (HDAC) complexes; APCC(Cdh1) substrate; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication
4306YLR184WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4307YLR185WRPL37ARibosomal 60S subunit protein L37ARibosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
4308YLR186WEMG1Methyltransferase for rRNAMethyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
4309YLR187WSKG3Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
4310YLR188WMDL1Mitochondrial inner membrane half-type ABC transporterMitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress
4311YLR189CATG26UDP-glucose:sterol glucosyltransferaseUDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
4312YLR190WMMR1Phosphorylated protein of the mitochondrial outer membranePhosphorylated protein of the mitochondrial outer membrane; localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
4313YLR191WPEX13Peroxisomal importomer complex componentPeroxisomal importomer complex component; integral peroxisomal membrane protein required for docking and translocation of peroxisomal matrix proteins; interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p; forms a complex with Pex14p and Pex17p
4314YLR192CHCR1eIF3j component of translation initiation factor 3 (eIF3)eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for processing of 20S pre-rRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
4315YLR193CUPS1Phosphatidic acid transfer proteinPhosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI
4316YLR194CNCW2Structural constituent of the cell wallStructural constituent of the cell wall; attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress
4317YLR195CNMT1N-myristoyl transferaseN-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
4318YLR196WPWP1Protein with WD-40 repeats involved in rRNA processingProtein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
4319YLR197WNOP56Essential evolutionarily-conserved nucleolar proteinEssential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
4320YLR198CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
4321YLR199CPBA1Protein involved in 20S proteasome assemblyProtein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
4322YLR200WYKE2Subunit of the heterohexameric Gim/prefoldin protein complexSubunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
4323YLR201CCOQ9Protein required for ubiquinone biosynthesis and respiratory growthProtein required for ubiquinone biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; ubiquinone is also known as coenzyme Q
4324YLR202CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
4325YLR203CMSS51Specific translational activator for the mitochondrial COX1 mRNASpecific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis
4326YLR204WQRI5Mitochondrial inner membrane proteinMitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
4327YLR205CHMX1ER localized heme oxygenaseER localized heme oxygenase; involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
4328YLR206WENT2Epsin-like protein required for endocytosis and actin patch assemblyEpsin-like protein required for endocytosis and actin patch assembly; functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; ENT2 has a paralog, ENT1, that arose from the whole genome duplication
4329YLR207WHRD3ER membrane protein that plays a central role in ERADER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events
4330YLR208WSEC13Structural component of 3 complexesStructural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
4331YLR209CPNP1Purine nucleoside phosphorylasePurine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
4332YLR210WCLB4B-type cyclin involved in cell cycle progressionB-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
4333YLR211CATG38Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex IHomodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy
4334YLR212CTUB4Gamma-tubulinGamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
4335YLR213CCRR1Putative glycoside hydrolase of the spore wall envelopePutative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
4336YLR214WFRE1Ferric reductase and cupric reductaseFerric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels
4337YLR215CCDC123Assembly factor for the eIF2 translation initiation factor complexProtein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
4338YLR216CCPR6Peptidyl-prolyl cis-trans isomerase (cyclophilin)Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
4339YLR217WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6
4340YLR218CCOA4Twin Cx(9)C protein involved in cytochrome c oxidase organizationTwin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors
4341YLR219WMSC3Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress
4342YLR220WCCC1Vacuolar Fe2+/Mn2+ transporterVacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress
4343YLR221CRSA3Protein with a likely role in ribosomal maturationProtein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
4344YLR222CUTP13Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
4345YLR222C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene IFH1
4346YLR223CIFH1Coactivator, regulates transcription of ribosomal protein (RP) genesCoactivator, regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing; regulated by acetylation and phosphorylation at different growth states via TORC1 signaling; IFH1 has a paralog, CRF1, that arose from the whole genome duplication
4347YLR224WUCC1F-box protein and component of SCF ubiquitin ligase complexesF-box protein and component of SCF ubiquitin ligase complexes; involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
4348YLR225CYLR225CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication
4349YLR226WBUR2Cyclin for the Sgv1p (Bur1p) protein kinaseCyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription
4350YLR227CADY4Structural component of the meiotic outer plaqueStructural component of the meiotic outer plaque; outer plaque is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane
4351YLR227W-AYLR227W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4352YLR227W-BYLR227W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4353YLR228CECM22Sterol regulatory element binding proteinSterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
4354YLR229CCDC42Small rho-like GTPaseSmall rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins
4355YLR230WDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42/YLR229C gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
4356YLR231CBNA5KynureninaseKynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
4357YLR232WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
4358YLR233CEST1TLC1 RNA-associated factor involved in telomere length regulationTLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication
4359YLR234WTOP3DNA Topoisomerase IIIDNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity stimulated by RPA and Sgs1p-Top2p-Rmi1p; involved in telomere stability and regulation of mitotic recombination
4360YLR235CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs TOP3/YLR234W and YLR236C
4361YLR236CYLR236CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YLR235C
4362YLR237WTHI7Plasma membrane transporter responsible for the uptake of thiaminePlasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
4363YLR238WFAR10Protein involved in recovery from arrest in response to pheromoneProtein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; FAR10 has a paralog, VPS64, that arose from the whole genome duplication
4364YLR239CLIP2Lipoyl ligaseLipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
4365YLR240WVPS34Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphatePhosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
4366YLR241WCSC1Calcium permeable gated cation channelCalcium permeable gated cation channel; may be involved in detoxification; similar to Arabidopsis CSC1
4367YLR242CARV1Cortical ER proteinCortical ER protein; implicated in the membrane insertion of tail-anchored C-terminal single transmembrane domain proteins; may function in transport of glycosylphosphatidylinositol intermediates into ER lumen; required for normal intracellular sterol distribution; human ARV1 required for normal cholesterol and bile acid homeostasis; similar to Nup120p
4368YLR243WGPN3Putative GTPase with a role in biogenesis of RNA pol II and polIIIPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; may have a role in sister chromatid cohesion; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
4369YLR244CMAP1Methionine aminopeptidaseMethionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
4370YLR245CCDD1Cytidine deaminaseCytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm
4371YLR246WERF2Subunit of a palmitoyltransferaseSubunit of a palmitoyltransferase; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; palmitoyltransferase is composed of Erf2p and Shr5p
4372YLR247CIRC20E3 ubiquitin ligase and putative helicaseE3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci
4373YLR248WRCK2Protein kinase involved in response to oxidative and osmotic stressProtein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
4374YLR249WYEF3Translation elongation factor 3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
4375YLR250WSSP120Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
4376YLR251WSYM1Protein required for ethanol metabolismProtein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
4377YLR252WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism
4378YLR253WMCP2Mitochondrial protein of unknown function involved in lipid homeostasMitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1
4379YLR254CNDL1Homolog of nuclear distribution factor NudEHomolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends
4380YLR255CYLR255CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4381YLR256WHAP1Zinc finger transcription factorZinc finger transcription factor; involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus
4382YLR256W-AYLR256W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4383YLR257WYLR257WProtein of unknown functionProtein of unknown function; protein abundance increases in response to DNA replication stress
4384YLR258WGSY2Glycogen synthaseGlycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
4385YLR259CHSP60Tetradecameric mitochondrial chaperoninTetradecameric mitochondrial chaperonin; required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
4386YLR260WLCB5Minor sphingoid long-chain base kinaseMinor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication
4387YLR261CVPS63Putative protein of unknown functionPutative protein of unknown function; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect; decreased levels of protein in enolase deficient mutant
4388YLR262CYPT6Rab family GTPaseRab family GTPase; Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; resides temporarily at the Golgi, dissociates into cytosol upon arrival of the Rab GTPaseYpt32p, which also functions in the late Golgi; Golgi-localized form is bound to GTP, while cytosolic form is GDP-bound; homolog of the mammalian Rab6
4389YLR262C-ATMA7Protein of unknown that associates with ribosomesProtein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
4390YLR263WRED1Protein component of the synaptonemal complex axial elementsProtein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p;
4391YLR264C-AYLR264C-APutative protein of unknown functionPutative protein of unknown function
4392YLR264WRPS28BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
4393YLR265CNEJ1Protein involved in regulation of nonhomologous end joiningProtein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p
4394YLR266CPDR8Transcription factorTranscription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
4395YLR267WBOP2Protein of unknown functionProtein of unknown function
4396YLR268WSEC22R-SNARE proteinR-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
4397YLR269CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4398YLR270WDCS1Non-essential hydrolase involved in mRNA decappingNon-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication
4399YLR271WCMG1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
4400YLR272CYCS4Subunit of the condensin complexSubunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
4401YLR273CPIG1Putative targeting subunit for type-1 protein phosphatase Glc7pPutative targeting subunit for type-1 protein phosphatase Glc7p; tethers Glc7p to Gsy2p glycogen synthase; PIG1 has a paralog, GAC1, that arose from the whole genome duplication
4402YLR274WMCM5Component of the Mcm2-7 hexameric helicase complexComponent of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
4403YLR275WSMD2Core Sm protein Sm D2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
4404YLR276CDBP9DEAD-box protein required for 27S rRNA processingDEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
4405YLR277CYSH1EndoribonucleasePutative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
4406YLR278CYLR278CZinc-cluster proteinZinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene
4407YLR279WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4408YLR280CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4409YLR281CRSO55Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
4410YLR282CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
4411YLR283WYLR283WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene
4412YLR284CECI1Peroxisomal delta3,delta2-enoyl-CoA isomerasePeroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; ECI1 has a paralog, DCI1, that arose from the whole genome duplication
4413YLR285C-AYLR285C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
4414YLR285WNNT1S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
4415YLR286CCTS1EndochitinaseEndochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
4416YLR286W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene CTS1
4417YLR287CYLR287CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
4418YLR287C-ARPS30AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
4419YLR288CMEC3DNA damage and meiotic pachytene checkpoint proteinDNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
4420YLR289WGUF1Mitochondrial matrix GTPaseMitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
4421YLR290CCOQ11Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexesPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
4422YLR291CGCD7Beta subunit of the translation initiation factor eIF2BBeta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
4423YLR292CSEC72Non-essential subunit of Sec63 complexNon-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p
4424YLR293CGSP1Ran GTPaseRan GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication
4425YLR294CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14
4426YLR295CATP14Subunit h of the F0 sector of mitochondrial F1F0 ATP synthaseSubunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
4427YLR296WYLR296WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4428YLR297WYLR297WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
4429YLR298CYHC1Component of the U1 snRNP complex required for pre-mRNA splicingComponent of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
4430YLR299C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene ECM38
4431YLR299WECM38Gamma-glutamyltranspeptidaseGamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation
4432YLR300WEXG1Major exo-1,3-beta-glucanase of the cell wallMajor exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication
4433YLR301WHRI1Protein of unknown function that interacts with Sec72p and Hrr25pProtein of unknown function that interacts with Sec72p and Hrr25p
4434YLR302CYLR302CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4435YLR303WMET17O-acetyl homoserine-O-acetyl serine sulfhydrylaseO-acetyl homoserine-O-acetyl serine sulfhydrylase; required for Methionine and cysteine biosynthesis
4436YLR304CACO1AconitaseAconitase; required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
4437YLR305CSTT4Phosphatidylinositol-4-kinasePhosphatidylinositol-4-kinase; functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
4438YLR306WUBC12Enzyme that mediates the conjugation of Rub1pEnzyme that mediates the conjugation of Rub1p; a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
4439YLR307C-AYLR307C-APutative protein of unknown functionPutative protein of unknown function
4440YLR307WCDA1Chitin deacetylaseChitin deacetylase; together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
4441YLR308WCDA2Chitin deacetylaseChitin deacetylase; together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
4442YLR309CIMH1Protein involved in vesicular transportProtein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
4443YLR310CCDC25Membrane bound guanine nucleotide exchange factorMembrane bound guanine nucleotide exchange factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; a membrane bound guanine nucleotide exchange factor is also known as a GEF or GDP-release factor
4444YLR311CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4445YLR312CATG39Autophagy receptor with a role in degradation of the ER and nucleusPutative protein of unknown function
4446YLR312W-AMRPL15Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
4447YLR313CSPH1Protein involved in shmoo formation and bipolar bud site selectionProtein involved in shmoo formation and bipolar bud site selection; localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; SPH1 has a paralog, SPA2, that arose from the whole genome duplication
4448YLR314CCDC3Component of the septin ring that is required for cytokinesisComponent of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
4449YLR315WNKP2Central kinetochore protein and subunit of the Ctf19 complexCentral kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
4450YLR316CTAD3Subunit of tRNA-specific adenosine-34 deaminaseSubunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
4451YLR317WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
4452YLR318WEST2Reverse transcriptase subunit of the telomerase holoenzymeReverse transcriptase subunit of the telomerase holoenzyme; essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
4453YLR319CBUD6Actin- and formin-interacting proteinActin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
4454YLR320WMMS22Subunit of E3 ubiquitin ligase complex involved in replication repairSubunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
4455YLR321CSFH1Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
4456YLR322WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth
4457YLR323CCWC24General splicing factorGeneral splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p
4458YLR324WPEX30ER-resident protein involved in peroxisomal biogenesisPeroxisomal integral membrane protein; involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p; PEX30 has a paralog, PEX31, that arose from the whole genome duplication
4459YLR325CRPL38Ribosomal 60S subunit protein L38Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
4460YLR326WYLR326WPutative protein of unknown functionPutative protein of unknown function; predicted to be palmitoylated
4461YLR327CTMA10Protein of unknown function that associates with ribosomesProtein of unknown function that associates with ribosomes; protein abundance increases in response to DNA replication stress; TMA10 has a paralog, STF2, that arose from the whole genome duplication
4462YLR328WNMA1Nicotinic acid mononucleotide adenylyltransferaseNicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
4463YLR329WREC102Protein involved in early stages of meiotic recombinationProtein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
4464YLR330WCHS5Component of the exomer complexComponent of the exomer complex; exomer also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus
4465YLR331CJIP3Putative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
4466YLR332WMID2O-glycosylated plasma membrane proteinO-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication
4467YLR333CRPS25BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication
4468YLR334CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here
4469YLR335WNUP2Nucleoporin involved in nucleocytoplasmic transportNucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization
4470YLR336CSGD1Essential nuclear proteinEssential nuclear protein; required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
4471YLR337CVRP1Verprolin, proline-rich actin-associated proteinVerprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)
4472YLR338WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
4473YLR339CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
4474YLR340WRPP0Conserved ribosomal protein P0 of the ribosomal stalkConserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
4475YLR341WSPO77Meiosis-specific protein of unknown functionMeiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
4476YLR342WFKS1Catalytic subunit of 1,3-beta-D-glucan synthaseCatalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication
4477YLR342W-AYLR342W-APutative protein of unknown functionPutative protein of unknown function
4478YLR343WGAS21,3-beta-glucanosyltransferase1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p
4479YLR344WRPL26ARibosomal 60S subunit protein L26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
4480YLR345WYLR345WSimilar to 6-phosphofructo-2-kinase enzymesSimilar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
4481YLR346CCIS1Protein of unknown function found in mitochondriaPutative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication
4482YLR347CKAP95Karyopherin betaKaryopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
4483YLR347W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF KAP95/YLR347C
4484YLR348CDIC1Mitochondrial dicarboxylate carrierMitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
4485YLR349WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
4486YLR350WORM2Protein that mediates sphingolipid homeostasisProtein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication
4487YLR351CNIT3Nit proteinNit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
4488YLR352WYLR352WPutative protein of unknown function with similarity to F-box proteinsPutative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene
4489YLR353WBUD8Protein involved in bud-site selectionProtein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication
4490YLR354CTAL1Transaldolase, enzyme in the non-oxidative pentose phosphate pathwayTransaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication
4491YLR355CILV5Acetohydroxyacid reductoisomerase and mtDNA binding proteinAcetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
4492YLR356WATG33Mitochondrial mitophagy-specific proteinMitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication
4493YLR357WRSC2Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication
4494YLR358CYLR358CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
4495YLR359WADE13Adenylosuccinate lyaseAdenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
4496YLR360WVPS38Part of a Vps34p phosphatidylinositol 3-kinase complexPart of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
4497YLR361CDCR2Protein phosphatasePhosphoesterase; involved in downregulation of the unfolded protein response (UPR), at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START
4498YLR361C-AYLR361C-APutative protein of unknown functionPutative protein of unknown function
4499YLR362WSTE11Signal transducing MEK kinaseSignal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress
4500YLR363CNMD4Protein that may be involved in nonsense-mediated mRNA decayProtein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
4501YLR363W-AYLR363W-AProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
4502YLR364C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR366W
4503YLR364WGRX8Glutaredoxin that employs a dithiol mechanism of catalysisGlutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
4504YLR365WYLR365WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene
4505YLR366WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
4506YLR367WRPS22BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
4507YLR368WMDM30F-box component of an SCF ubiquitin protein ligase complexF-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
4508YLR369WSSQ1Mitochondrial hsp70-type molecular chaperoneMitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
4509YLR370CARC18Subunit of the ARP2/3 complexSubunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
4510YLR371WROM2Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2pGDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
4511YLR372WELO3ElongaseElongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
4512YLR373CVID22Glycosylated integral membrane protein localized to plasma membraneGlycosylated integral membrane protein localized to plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles; VID22 has a paralog, ENV11, that arose from the whole genome duplication
4513YLR374CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
4514YLR375WSTP3Zinc-finger protein of unknown functionZinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication
4515YLR376CPSY3Component of Shu complex (aka PCSS complex)Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
4516YLR377CFBP1Fructose-1,6-bisphosphataseFructose-1,6-bisphosphatase; key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; glucose starvation results in redistribution to the periplasm; interacts with Vid30p
4517YLR378CSEC61Conserved ER protein translocation channelConserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
4518YLR379WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C
4519YLR380WCSR1Phosphatidylinositol transfer proteinPhosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
4520YLR381WCTF3Outer kinetochore protein that forms a complex with Mcm16p and Mcm22pOuter kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6
4521YLR382CNAM2Mitochondrial leucyl-tRNA synthetaseMitochondrial leucyl-tRNA synthetase; also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
4522YLR383WSMC6Component of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
4523YLR384CIKI3Subunit of Elongator complexSubunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
4524YLR385CSWC7Protein of unknown functionProtein of unknown function; component of the Swr1p complex that incorporates Htz1p into chromatin
4525YLR386WVAC14Enzyme regulatorEnzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
4526YLR387CREH1Cytoplasmic 60S subunit biogenesis factorCytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
4527YLR388WRPS29AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
4528YLR389CSTE23MetalloproteaseMetalloprotease; involved in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family; homolog Axl1p is also involved in processing of pro-a-factor
4529YLR390WECM19Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
4530YLR390W-ACCW14Covalently linked cell wall glycoproteinCovalently linked cell wall glycoprotein; present in the inner layer of the cell wall
4531YLR392CART10Protein of unknown function that contains 2 PY motifsProtein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
4532YLR393WATP10Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthaseAssembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6
4533YLR394WCST9SUMO E3 ligaseSUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate
4534YLR395CCOX8Subunit VIII of cytochrome c oxidase (Complex IV)Subunit VIII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
4535YLR396CVPS33ATP-binding protein that is a subunit of the HOPS and CORVET complexesATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains
4536YLR397CAFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complexATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs
4537YLR398CSKI2Ski complex component and putative RNA helicaseSki complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
4538YLR399CBDF1Protein involved in transcription initiationProtein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication
4539YLR399W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the characterized ORF BDF1/YLR399C
4540YLR400WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4541YLR401CDUS3Dihydrouridine synthaseDihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
4542YLR402WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4543YLR403WSFP1Regulates transcription of ribosomal protein and biogenesis genesRegulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
4544YLR404WSEI1Seipin involved in lipid droplet (LD) assemblySeipin protein; involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
4545YLR405WDUS4Dihydrouridine synthaseDihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
4546YLR406CRPL31BRibosomal 60S subunit protein L31BRibosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
4547YLR406C-AYLR406C-APutative protein of unknown functionPutative protein of unknown function
4548YLR407WYLR407WPutative protein of unknown functionPutative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
4549YLR408CBLS1Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
4550YLR409CUTP21Subunit of U3-containing 90S preribosome and SSU processome complexesSubunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome
4551YLR410WVIP1Inositol hexakisphosphate and inositol heptakisphosphate kinaseInositol hexakisphosphate and inositol heptakisphosphate kinase; inositol heptakisphosphate (IP7) production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1; inositol hexakisphosphate is also known as IP6
4552YLR410W-AYLR410W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4553YLR410W-BYLR410W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4554YLR411WCTR3High-affinity copper transporter of the plasma membraneHigh-affinity copper transporter of the plasma membrane; acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae
4555YLR412C-AYLR412C-APutative protein of unknown functionPutative protein of unknown function
4556YLR412WBER1Protein involved in microtubule-related processesProtein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
4557YLR413WINA1Protein of unknown functionPutative protein of unknown function; not an essential gene; YLR413W has a paralog, FAT3, that arose from the whole genome duplication
4558YLR414CPUN1Plasma membrane protein with a role in cell wall integrityPlasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
4559YLR415CYLR415CPutative protein of unknown functionPutative protein of unknown function; YLR415C is not an essential gene
4560YLR416CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4561YLR417WVPS36Component of the ESCRT-II complexComponent of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome; plays a role in the formation of mutant huntingtin (Htt) aggregates in yeast
4562YLR418CCDC73Component of the Paf1p complexComponent of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
4563YLR419WYLR419WPutative helicase with limited sequence similarity to human Rb proteinPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
4564YLR420WURA4DihydroorotaseDihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
4565YLR421CRPN13Subunit of the 19S regulatory particle of the 26S proteasome lidSubunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
4566YLR422WDCK1Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1Protein of unknown function with similarity to human DOCK proteins; interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein; DOCK proteins act as guanine nucleotide exchange factors
4567YLR423CATG17Scaffold protein responsible for phagophore assembly site organizationScaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
4568YLR424WSPP382Essential protein that forms a dimer with Ntr2pEssential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
4569YLR425WTUS1Guanine nucleotide exchange factor (GEF) that modulates Rho1p activityGuanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
4570YLR426WTDA5Putative protein of unknown functionPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
4571YLR427WMAG2Cytoplasmic protein of unknown functionCytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
4572YLR428CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1
4573YLR429WCRN1CoroninCoronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
4574YLR430WSEN1ATP-dependent 5' to 3' RNA/DNA and DNA helicasePresumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
4575YLR431CATG23Peripheral membrane protein required for autophagy and CVTPeripheral membrane protein required for autophagy and CVT; required for cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
4576YLR432WIMD3Inosine monophosphate dehydrogenaseInosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication
4577YLR433CCNA1Calcineurin ACalcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication
4578YLR434CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
4579YLR435WTSR2Protein with a potential role in pre-rRNA processingProtein with a potential role in pre-rRNA processing
4580YLR436CECM30Protein of unknown functionPutative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
4581YLR437CDIF1Protein that regulates nuclear localization of Rnr2p and Rnr4pProtein that regulates nuclear localization of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication
4582YLR437C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CAR2/YLR438W
4583YLR438C-ALSM3Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
4584YLR438WCAR2L-ornithine transaminase (OTAse)L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress
4585YLR439WMRPL4Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
4586YLR440CSEC39Component of the Dsl1p tethering complexComponent of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
4587YLR441CRPS1ARibosomal protein 10 (rp10) of the small (40S) subunitRibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
4588YLR442CSIR3Silencing proteinSilencing protein; interacts with Sir2p, Sir4p, and histone H3 and H4 tails to establish transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus (residues 840-978) assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; SIR3 has a paralog, ORC1, that arose from the whole genome duplication
4589YLR443WECM7Putative integral membrane protein with a role in calcium uptakePutative integral membrane protein with a role in calcium uptake; non-essential protein; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
4590YLR444CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4591YLR445WGMC2Protein involved in meiotic crossing overProtein involved in meiotic crossing over; component of the Synaptonemal Complex (SC) along with Ecm11p; required for the efficient loading of the SC transverse filament protein, Zip1p; promotes SUMOylation of Ecm11p; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor
4592YLR446WYLR446WPutative hexokinasePutative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene
4593YLR447CVMA6Subunit d of the V0 integral membrane domain of V-ATPaseSubunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits
4594YLR448WRPL6BRibosomal 60S subunit protein L6BRibosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
4595YLR449WFPR4Peptidyl-prolyl cis-trans isomerase (PPIase)Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR4 has a paralog, FPR3, that arose from the whole genome duplication
4596YLR450WHMG2HMG-CoA reductaseHMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication
4597YLR451WLEU3Zinc-knuckle transcription factor, repressor and activatorZinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
4598YLR452CSST2GTPase-activating protein for Gpa1pGTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
4599YLR453CRIF2Protein that binds to the Rap1p C-terminusProtein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication
4600YLR454WFMP27Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
4601YLR455WPDP3Component of the NuA3b histone acetyltransferase complexComponent of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
4602YLR456WYLR456WProtein of unknown functionPutative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
4603YLR457CNBP1Spindle pole body (SPB) componentSpindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
4604YLR458WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1/YLR457C gene required for mitosis
4605YLR459WGAB1GPI transamidase subunitGPI transamidase subunit; involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
4606YLR460CYLR460CMember of the quinone oxidoreductase familyMember of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
4607YLR461WPAU4Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
4608YLR462WYLR462WPutative protein of unknown function with similarity to helicasesPutative protein of unknown function with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII
4609YLR463CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORFs YLR462W and YLR464W
4610YLR464WYLR464WPutative protein of unknown functionPutative protein of unknown function; intron is predicted but not detected experimentally; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C
4611YLR465CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 100% of YLR465C overlaps the uncharacterized ORF YLR464W and 86% of YLR465C overlaps the verified gene YRF1-4
4612YLR466C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W
4613YLR466C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4614YLR466WYRF1-4Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
4615YLR467C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W
4616YLR467WYRF1-5Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
4617YML001WYPT7Rab family GTPaseRab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
4618YML002WYML002WPutative protein of unknown functionPutative protein of unknown function; expression induced by heat and by calcium shortage
4619YML003WYML003WPutative protein of unknown functionPutative protein of unknown function
4620YML004CGLO1Monomeric glyoxalase IMonomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
4621YML005WTRM12S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family
4622YML006CGIS4CAAX box containing protein of unknown functionCAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
4623YML007C-AMIN4Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
4624YML007WYAP1Basic leucine zipper (bZIP) transcription factorBasic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication
4625YML008CERG6Delta(24)-sterol C-methyltransferaseDelta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane
4626YML009CMRPL39Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
4627YML009C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs SPT5/YML010W and YML009W-B
4628YML009W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
4629YML010WSPT5Spt4p/5p (DSIF) transcription elongation factor complex subunitComponent of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
4630YML011CRAD33Protein involved in nucleotide excision repairProtein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
4631YML012C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
4632YML012WERV25Member of the p24 family involved in ER to Golgi transportMember of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24,
4633YML013WUBX2Bridging factor involved in ER-associated protein degradation (ERAD)Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
4634YML014WTRM9tRNA methyltransferasetRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
4635YML015CTAF11TFIID subunit (40 kDa)TFIID subunit (40 kDa); involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
4636YML016CPPZ1Serine/threonine protein phosphatase Z, isoform of Ppz2pSerine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
4637YML017WPSP2Asn rich cytoplasmic protein that contains RGG motifsAsn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
4638YML018CYML018CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
4639YML019WOST6Subunit of the oligosaccharyltransferase complex of the ER lumenSubunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
4640YML020WYML020WPutative protein of unknown functionPutative protein of unknown function
4641YML021CUNG1Uracil-DNA glycosylaseUracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus
4642YML022WAPT1Adenine phosphoribosyltransferaseAdenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
4643YML023CNSE5Component of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
4644YML024WRPS17ARibosomal protein 51 (rp51) of the small (40s) subunitRibosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication
4645YML025CYML6Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
4646YML026CRPS18BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
4647YML027WYOX1Homeobox transcriptional repressorHomeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
4648YML028WTSA1Thioredoxin peroxidaseThioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; required for telomere length maintenance; protein abundance increases, forms cytoplasmic foci during DNA replication stress; TSA1 has a paralog, TSA2, that arose from the whole genome duplication
4649YML029WUSA1Scaffold subunit of the Hrd1p ubiquitin ligaseScaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
4650YML030WRCF1Cytochrome c oxidase subunitCytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
4651YML031C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF NDC1/YML031W
4652YML031WNDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC)Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
4653YML032CRAD52Protein that stimulates strand exchangeProtein that stimulates strand exchange; stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination
4654YML034C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SRC1/YML034W
4655YML034WSRC1Inner nuclear membrane proteinInner nuclear membrane protein; highly enriched at telomeres and subtelomeric regions; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance; SEC1 has a paralog, HEH2, that arose from the whole genome duplication
4656YML035CAMD1AMP deaminaseAMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
4657YML036WCGI121Component of the EKC/KEOPS complexComponent of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p
4658YML037CYML037CPutative protein of unknown functionPutative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
4659YML038CYMD8Putative nucleotide sugar transporterPutative nucleotide sugar transporter; has similarity to Vrg4p
4660YML039WYML039WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4661YML040WYML040WRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4662YML041CVPS71Nucleosome-binding component of the SWR1 complexNucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
4663YML042WCAT2Carnitine acetyl-CoA transferaseCarnitine acetyl-CoA transferase; present in both mitochondria and peroxisomes; transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
4664YML043CRRN11Component of the core factor (CF) rDNA transcription factor complexComponent of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
4665YML045WYML045WRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4666YML045W-AYML045W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4667YML046WPRP39U1 snRNP protein involved in splicingU1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
4668YML047CPRM6Potassium transporter that mediates K+ influxPotassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; member of a fungal-specific gene family; PRM6 has a paralog, KCH1, that arose from the whole genome duplication
4669YML047W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF PRM6/YML047C
4670YML048WGSF2Endoplasmic reticulum (ER) localized integral membrane proteinEndoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
4671YML049CRSE1Protein involved in pre-mRNA splicingProtein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
4672YML050WAIM32Protein of unknown functionPutative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss
4673YML051WGAL80Transcriptional regulator involved in the repression of GAL genesTranscriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
4674YML052WSUR7Plasma membrane protein, component of eisosomesPlasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches)
4675YML053CYML053CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
4676YML054CCYB2Cytochrome b2 (L-lactate cytochrome-c oxidoreductase)Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
4677YML054C-AYML054C-APutative protein of unknown functionPutative protein of unknown function
4678YML055WSPC2Subunit of signal peptidase complexSubunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p
4679YML056CIMD4Inosine monophosphate dehydrogenaseInosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication
4680YML057C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
4681YML057WCMP2Calcineurin ACalcineurin A; one isoform (the other is Cna1p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CMP2 has a paralog, CNA1, that arose from the whole genome duplication
4682YML058WSML1Ribonucleotide reductase inhibitorRibonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication
4683YML058W-AHUG1Ribonucleotide reductase inhibitorProtein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress
4684YML059CNTE1Serine esteraseSerine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
4685YML060WOGG1Nuclear and mitochondrial glycosylase/lyaseNuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
4686YML061CPIF1DNA helicase, potent G-quadruplex DNA binder/unwinderDNA helicase, potent G-quadruplex DNA binder/unwinder; promotes DNA synthesis during break-induced replication (BIR); important for crossover recombination; translated from different start sites for two forms localized to either mitochondria or nucleus; nuclear form is catalytic inhibitor of telomerase; mitochondrial form involved in mitochondrial DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
4687YML062CMFT1Subunit of the THO complexSubunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
4688YML063WRPS1BRibosomal protein 10 (rp10) of the small (40S) subunitRibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
4689YML064CTEM1GTP-binding protein of the Ras superfamilyGTP-binding protein of the Ras superfamily; involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
4690YML065WORC1Largest subunit of the origin recognition complexLargest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; ORC1 has a paralog, SIR3, that arose from the whole genome duplication
4691YML066CSMA2Meiosis-specific prospore membrane proteinMeiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation
4692YML067CERV41Protein localized to COPII-coated vesiclesProtein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
4693YML068WITT1Protein that modulates the efficiency of translation terminationProtein that modulates the efficiency of translation termination; interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
4694YML069WPOB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p)Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
4695YML070WDAK1Dihydroxyacetone kinaseDihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
4696YML071CCOG8Component of the conserved oligomeric Golgi complexComponent of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
4697YML072CTCB3Cortical ER protein involved in ER-plasma membrane tetheringCortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
4698YML073CRPL6ARibosomal 60S subunit protein L6ARibosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
4699YML074CFPR3Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase)Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication
4700YML075CHMG1HMG-CoA reductaseHMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication
4701YML076CWAR1Homodimeric Zn2Cys6 zinc finger transcription factorHomodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
4702YML077WBET5Core component of transport protein particle (TRAPP) complexes I-IIICore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII)
4703YML078WCPR3Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin)Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
4704YML079WYML079WNon-essential protein of unknown functionNon-essential protein of unknown function; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm
4705YML080WDUS1Dihydrouridine synthaseDihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
4706YML081C-AATP18Subunit of the mitochondrial F1F0 ATP synthaseSubunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
4707YML081WTDA9Transcription factor that regulates acetate productionTranscription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
4708YML082WYML082WPutative protein predicted to have carbon-sulfur lyase activityPutative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; not an essential gene; YML082W has a paralog, STR2, that arose from the whole genome duplication
4709YML083CYML083CProtein of unknown functionProtein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
4710YML084WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4711YML085CTUB1Alpha-tubulinAlpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
4712YML086CALO1D-Arabinono-1,4-lactone oxidaseD-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
4713YML087CAIM33Protein of unknown function, highly conserved across speciesPutative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication
4714YML088WUFO1F-box receptor proteinF-box receptor protein; subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
4715YML089CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage
4716YML090WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source
4717YML091CRPM2Protein subunit of mitochondrial RNase PProtein subunit of mitochondrial RNase P; has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
4718YML092CPRE8Alpha 2 subunit of the 20S proteasomeAlpha 2 subunit of the 20S proteasome
4719YML093WUTP14Subunit of U3-containing Small Subunit (SSU) processome complexSubunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
4720YML094C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
4721YML094WGIM5Subunit of the heterohexameric cochaperone prefoldin complexSubunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
4722YML095CRAD10Single-stranded DNA endonuclease (with Rad1p)Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
4723YML096WYML096WPutative protein with similarity to asparagine synthetasesPutative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
4724YML097CVPS9Guanine nucleotide exchange factor (GEF) and ubiquitin receptorGuanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a VPS9 domain; binds ubiquitin
4725YML098WTAF13TFIID subunit (19 kDa)TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
4726YML099CARG81Zinc finger transcription factor involved in arginine-responsive genesZinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
4727YML099W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ARG81/YML099C
4728YML100WTSL1Large subunit of trehalose 6-phosphate synthase/phosphatase complexLarge subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
4729YML100W-AYML100W-APutative protein of unknown functionPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
4730YML101CCUE4Protein of unknown functionProtein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication
4731YML101C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4732YML102WCAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1pSubunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia
4733YML103CNUP188Subunit of the inner ring of the nuclear pore complex (NPC)Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
4734YML104CMDM1PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJsIntermediate filament protein; required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P)
4735YML105CSEC65Subunit of the signal recognition particle (SRP)Subunit of the signal recognition particle (SRP); involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
4736YML106WURA5Major orotate phosphoribosyltransferase (OPRTase) isozymeMajor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
4737YML107CPML39Protein required for nuclear retention of unspliced pre-mRNAsProtein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes
4738YML108WYML108WProtein of unknown functionProtein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
4739YML109WZDS2Protein with a role in regulating Swe1p-dependent polarized growthProtein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication
4740YML110CCOQ52-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
4741YML111WBUL2Component of the Rsp5p E3-ubiquitin ligase complexComponent of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication
4742YML112WCTK3Gamma subunit of C-terminal domain kinase IGamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
4743YML113WDAT1DNA binding protein that recognizes oligo(dA).oligo(dT) tractsDNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability
4744YML114CTAF8TFIID subunit (65 kDa)TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation
4745YML115CVAN1Component of the mannan polymerase IComponent of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
4746YML116WATR1Multidrug efflux pump of the major facilitator superfamilyMultidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
4747YML116W-APutative protein of unknown function
4748YML117WNAB6Putative RNA-binding proteinPutative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
4749YML118WNGL33'-5' exonuclease specific for poly-A RNAs3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
4750YML119WYML119WPutative protein of unknown functionPutative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
4751YML120CNDI1NADH:ubiquinone oxidoreductaseNADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID
4752YML121WGTR1Subunit of a TORC1-stimulating GTPase and the EGO/GSE complexCytoplasmic GTPase; forms a heterodimer with Gtr2p to stimulate TORC1 in response to amino acids; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
4753YML122CYML122CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4754YML123CPHO84High-affinity inorganic phosphate (Pi) transporterHigh-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
4755YML124CTUB3Alpha-tubulinAlpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
4756YML125CPGA3Putative cytochrome b5 reductase, localized to the plasma membranePutative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
4757YML126CERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
4758YML127WRSC9Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
4759YML128CMSC1Protein of unknown functionProtein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
4760YML129CCOX14Mitochondrial cytochrome c oxidase (complex IV) assembly factorMitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane
4761YML130CERO1Thiol oxidase required for oxidative protein folding in the ERThiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
4762YML131WYML131WProtein of unknown functionProtein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
4763YML132WCOS3Endosomal protein involved in turnover of plasma membrane proteinsProtein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
4764YML133CYML133CPutative Y' element ATP-dependent helicasePutative Y' element ATP-dependent helicase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron
4765YML133W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C
4766YML133W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C
4767YMR001CCDC5Polo-like kinasePolo-like kinase; controls targeting and activation of Rho1p at cell division site via Rholp guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
4768YMR001C-AYMR001C-APutative protein of unknown functionPutative protein of unknown function
4769YMR002WMIX17Mitochondrial intermembrane space proteinMitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
4770YMR003WAIM34Protein of unknown functionProtein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
4771YMR004WMVP1Protein required for sorting proteins to the vacuoleProtein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
4772YMR005WTAF4TFIID subunit (48 kDa)TFIID subunit (48 kDa); involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
4773YMR006CPLB2Phospholipase B (lysophospholipase) involved in lipid metabolismPhospholipase B (lysophospholipase) involved in lipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
4774YMR007WYMR007WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4775YMR008CPLB1Phospholipase B (lysophospholipase) involved in lipid metabolismPhospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication
4776YMR009WADI1Acireductone dioxygenease involved in methionine salvage pathwayAcireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions
4777YMR010WANY1Protein involved in phospholipid flippase functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1
4778YMR011WHXT2High-affinity glucose transporter of the major facilitator superfamilyHigh-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose
4779YMR012WCLU1Subunit of the eukaryotic translation initiation factor 3 (eIF3)Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant
4780YMR013CSEC59Dolichol kinaseDolichol kinase; catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
4781YMR013C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF SEC59/YML013C
4782YMR013W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73
4783YMR014WBUD22Protein required for rRNA maturation and ribosomal subunit biogenesisProtein required for rRNA maturation and ribosomal subunit biogenesis; required for 18S rRNA maturation; also required for small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern
4784YMR015CERG5C-22 sterol desaturaseC-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
4785YMR016CSOK2Nuclear protein that negatively regulates pseudohyphal differentiationNuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
4786YMR017WSPO20Meiosis-specific subunit of the t-SNARE complexMeiosis-specific subunit of the t-SNARE complex; required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
4787YMR018WPEX9Putative protein of unknown function with similarity to human PEX5RpPutative protein of unknown function with similarity to human PEX5Rp; transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene; PEX5Rp is also known as peroxin protein 5 related protein
4788YMR019WSTB4Putative transcription factorPutative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
4789YMR020WFMS1Polyamine oxidasePolyamine oxidase; converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
4790YMR021CMAC1Copper-sensing transcription factorCopper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS
4791YMR022WUBC7Ubiquitin conjugating enzymeUbiquitin conjugating enzyme; involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
4792YMR023CMSS1Mitochondrial proteinMitochondrial protein; forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
4793YMR024WMRPL3Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type
4794YMR025WCSI1Subunit of the Cop9 signalosomeSubunit of the Cop9 signalosome; which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
4795YMR026CPEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligaseC3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
4796YMR027WYMR027WA metal-dependent phosphatase, part of the DUF89 protein familyPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
4797YMR028WTAP42Essential protein involved in the TOR signaling pathwayEssential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
4798YMR029CFAR8Protein involved in recovery from arrest in response to pheromoneProtein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
4799YMR030WRSF1Protein required for respiratory growthProtein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
4800YMR030W-AYMR030W-APutative protein of unknown functionPutative protein of unknown function
4801YMR031CEIS1Component of the eisosome required for proper eisosome assemblyComponent of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication
4802YMR031W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
4803YMR032WHOF1Protein that regulates actin cytoskeleton organizationSH3 domain-containing protein required for cytokinesis; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p
4804YMR033WARP9Component of both the SWI/SNF and RSC chromatin remodeling complexesComponent of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
4805YMR034CRCH1Putative transporterPutative transporter; member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene
4806YMR035WIMP2Catalytic subunit of mitochondrial inner membrane peptidase complexCatalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificity), and Som1p
4807YMR036CMIH1Protein tyrosine phosphatase involved in cell cycle controlProtein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
4808YMR037CMSN2Stress-responsive transcriptional activatorStress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress
4809YMR038CCCS1Copper chaperone for superoxide dismutase Sod1pCopper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress
4810YMR039CSUB1Transcriptional regulatorTranscriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress
4811YMR040WYET2Protein of unknown function that may interact with ribosomesProtein of unknown function that may interact with ribosomes; based on co-purification experiments; homolog of human BAP31 protein; YET2 has a paralog, YET1, that arose from the whole genome duplication
4812YMR041CARA2NAD-dependent arabinose dehydrogenaseNAD-dependent arabinose dehydrogenase; involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
4813YMR042WARG80Transcription factor involved in regulating arginine-responsive genesTranscription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p
4814YMR043WMCM1Transcription factorTranscription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia
4815YMR044WIOC4Member of a complex (Isw1b) with Isw1p and Ioc2pMember of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
4816YMR045CYMR045CRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4817YMR046CYMR046CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4818YMR046W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4819YMR047CNUP116FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication
4820YMR048WCSM3Replication fork associated factorReplication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
4821YMR049CERB1Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
4822YMR050CYMR050CRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
4823YMR051CYMR051CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
4824YMR052C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4825YMR052WFAR3Protein of unknown functionProtein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
4826YMR053CSTB2Protein that interacts with Sin3p in a two-hybrid assayProtein that interacts with Sin3p in a two-hybrid assay; part of a large protein complex with Sin3p and Stb1p; STB2 has a paralog, STB6, that arose from the whole genome duplication
4827YMR054WSTV1Subunit a of the vacuolar-ATPase V0 domainSubunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
4828YMR055CBUB2Mitotic exit network regulatorMitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
4829YMR056CAAC1Mitochondrial inner membrane ADP/ATP translocatorMitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
4830YMR057CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
4831YMR058WFET3Ferro-O2-oxidoreductaseFerro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
4832YMR059WSEN15Subunit of the tRNA splicing endonucleaseSubunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p
4833YMR060CSAM37Component of the Sorting and Assembly Machinery (SAM) complexComponent of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
4834YMR061WRNA14Component of the cleavage and polyadenylation factor I (CF I)Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia
4835YMR062CARG7Mitochondrial ornithine acetyltransferaseMitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
4836YMR063WRIM9Plasma membrane protein of unknown functionPlasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
4837YMR064WAEP1Protein required for expression of the mitochondrial OLI1 geneProtein required for expression of the mitochondrial OLI1 gene; mitochondrial OLI1 gene encodes subunit 9 of F1-F0 ATP synthase
4838YMR065WKAR5Protein required for nuclear membrane fusion during karyogamyProtein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone
4839YMR066WSOV1Mitochondrial protein of unknown functionMitochondrial protein of unknown function
4840YMR067CUBX4UBX domain-containing protein that interacts with Cdc48pUBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
4841YMR068WAVO2Component of a complex containing the Tor2p kinase and other proteinsComponent of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth
4842YMR069WNAT4N alpha-acetyl-transferaseN alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A
4843YMR070WMOT3Transcriptional repressor, activatorTranscriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth and ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; relocalizes to the cytosol in response to hypoxia; can form [MOT3+] prion
4844YMR071CTVP18Integral membrane proteinIntegral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
4845YMR072WABF2Mitochondrial DNA-binding proteinMitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication
4846YMR073CIRC21Protein of unknown functionPutative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
4847YMR074CSDD2Protein with homology to human PDCD5Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
4848YMR075C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W
4849YMR075WRCO1Essential component of the Rpd3S histone deacetylase complexEssential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
4850YMR076CPDS5Cohesion maintenance factorCohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; also required during meiosis; relocalizes to the cytosol in response to hypoxia
4851YMR077CVPS20Myristoylated subunit of the ESCRT-III complexMyristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
4852YMR078CCTF18Subunit of a complex with Ctf8pSubunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
4853YMR079WSEC14Phosphatidylinositol/phosphatidylcholine transfer proteinPhosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
4854YMR080CNAM7ATP-dependent RNA helicase of the SFI superfamilyATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
4855YMR081CISF1Serine-rich, hydrophilic proteinSerine-rich, hydrophilic protein; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; ISF1 has a paralog, MBR1, that arose from the whole genome duplication
4856YMR082CYMR082CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4857YMR083WADH3Mitochondrial alcohol dehydrogenase isozyme IIIMitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
4858YMR084WYMR084WPutative protein of unknown functionPutative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
4859YMR085WYMR085WPutative protein of unknown functionPutative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
4860YMR086C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4861YMR086WSEG1Component of eisosome required for proper eisosome assemblyComponent of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication
4862YMR087WYMR087WPutative ADP-ribose-1''-monophosphatasePutative ADP-ribose-1''-monophosphatase; converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
4863YMR088CVBA1Permease of basic amino acids in the vacuolar membranePermease of basic amino acids in the vacuolar membrane
4864YMR089CYTA12Mitochondrial inner membrane m-AAA protease componentMitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes
4865YMR090WYMR090WPutative protein of unknown functionPutative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
4866YMR091CNPL6Component of the RSC chromatin remodeling complexComponent of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions; involved in nuclear protein import and maintenance of proper telomere length
4867YMR092CAIP1Actin cortical patch componentActin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
4868YMR093WUTP15Nucleolar proteinNucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
4869YMR094WCTF13Subunit of the CBF3 complexSubunit of the CBF3 complex; CBF3 binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
4870YMR095CSNO1Protein of unconfirmed functionProtein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
4871YMR096WSNZ1Protein involved in vitamin B6 biosynthesisProtein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins
4872YMR097CMTG1Putative GTPase peripheral to the mitochondrial inner membranePutative GTPase peripheral to the mitochondrial inner membrane; essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
4873YMR098CATP25Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNAMitochondrial protein required for the stability of Oli1p (Atp9p) mRNA; also required for the Oli1p ring formation; YMR098C is not an essential gene
4874YMR099CYMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase)Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase); likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
4875YMR100WMUB1MYND domain-containing proteinMYND domain-containing protein; required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene
4876YMR101CSRT1Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1Cis-prenyltransferase; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
4877YMR102CYMR102CProtein of unknown functionProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; not an essential gene; YMR102C has a paralog, DGR2, that arose from the whole genome duplication
4878YMR103CYMR103CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4879YMR104CYPK2Protein kinase similar to S/T protein kinase Ypk1pProtein kinase similar to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
4880YMR105CPGM2PhosphoglucomutasePhosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
4881YMR105W-AYMR105W-APutative protein of unknown functionPutative protein of unknown function
4882YMR106CYKU80Subunit of telomeric Ku complex (Yku70p-Yku80p)Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
4883YMR107WSPG4Protein required for high temperature survival during stationary phaseProtein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources
4884YMR108WILV2Acetolactate synthaseAcetolactate synthase; catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control
4885YMR109WMYO5One of two type I myosin motorsOne of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
4886YMR110CHFD1Dehydrogenase involved in ubiquinone and sphingolipid metabolismHexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
4887YMR111CYMR111CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
4888YMR112CMED11Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
4889YMR113WFOL3Dihydrofolate synthetase, involved in folic acid biosynthesisDihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication
4890YMR114CYMR114CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
4891YMR115WMGR3Subunit of the mitochondrial (mt) i-AAA protease supercomplexSubunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
4892YMR116CASC1G-protein beta subunit and guanine dissociation inhibitor for Gpa2pG-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
4893YMR117CSPC24Component of the kinetochore-associated Ndc80 complexComponent of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
4894YMR118CSHH3Putative mitochondrial inner membrane protein of unknown functionPutative mitochondrial inner membrane protein of unknown function; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication
4895YMR119WASI1Subunit of the inner nuclear membrane Asi ubiquitin ligase complexSubunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
4896YMR119W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of ORF ASI1/YMR119W
4897YMR120CADE17Enzyme of 'de novo' purine biosynthesisEnzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
4898YMR121CRPL15BRibosomal 60S subunit protein L15BRibosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
4899YMR122CYMR122CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4900YMR122W-AYMR122W-AProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and endoplasmic reticulum
4901YMR123WPKR1V-ATPase assembly factorV-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
4902YMR124WEPO1Protein involved in septin-ER tetheringProtein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication
4903YMR125WSTO1Large subunit of the nuclear mRNA cap-binding protein complexLarge subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
4904YMR126CDLT1Protein of unknown functionProtein of unknown function; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
4905YMR127CSAS2Histone acetyltransferase (HAT) catalytic subunit of the SAS complexHistone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p
4906YMR128WECM16Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNPEssential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; predominantly nucleolar in distribution; required for 18S rRNA synthesis
4907YMR129WPOM152Glycoprotein subunit of transmembrane ring of nuclear pore complexGlycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; homologous to human NUP210
4908YMR130WDPI35Putative protein of unknown functionPutative protein of unknown function; YMR130W is not an essential gene
4909YMR131CRRB1Specific chaperone for ribosomal protein Rpl3pNuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p; essential gene
4910YMR132CJLP2Protein of unknown functionProtein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
4911YMR133WREC114Protein involved in early stages of meiotic recombinationProtein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
4912YMR134WERG29Protein of unknown function involved in ergosterol biosynthesisProtein of unknown function involved in ergosterol biosynthesis; conditional mutants produce less ergosterol, display impaired oxygen consumption, respiratory growth, mitochondrial iron utilization, and are more sensitive to oxidative stress; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; protein localizes to the cytoplasm, ER and nuclear envelope; highly conserved in ascomycetes
4913YMR135CGID8Subunit of GID Complex, binds strongly to central component Vid30pSubunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
4914YMR135W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4915YMR136WGAT2Protein containing GATA family zinc finger motifsProtein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
4916YMR137CPSO2Nuclease required for DNA single- and double-strand break repairNuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
4917YMR138WCIN4GTP-binding protein involved in beta-tubulin (Tub2p) foldingGTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
4918YMR139WRIM11Protein kinaseProtein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication
4919YMR140WSIP5Protein of unknown functionProtein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
4920YMR141CYMR141CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4921YMR141W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene RPL13B/YMR142C
4922YMR142CRPL13BRibosomal 60S subunit protein L13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
4923YMR143WRPS16AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
4924YMR144WFDO1Protein involved in directionality of mating type switchingPutative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene
4925YMR145CNDE1Mitochondrial external NADH dehydrogenaseMitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication
4926YMR146CTIF34eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3)eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
4927YMR147WLDO45Putative protein of unknown functionPutative protein of unknown function
4928YMR148WOSW5Protein of unknown function with possible role in spore wall assemblyProtein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
4929YMR149WSWP1Delta subunit of the oligosaccharyl transferase glycoprotein complexDelta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum
4930YMR150CIMP1Catalytic subunit of mitochondrial inner membrane peptidase complexCatalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificty) and Som1p
4931YMR151WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
4932YMR152WYIM1Protein of unknown functionProtein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
4933YMR153C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of verified gene NUP53/YMR153W
4934YMR153WNUP53FG-nucleoporin component of central core of nuclear pore complex (NPC)FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
4935YMR154CRIM13Calpain-like cysteine proteaseCalpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
4936YMR155WYMR155WPutative protein of unknown functionPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen
4937YMR156CTPP1DNA 3'-phosphataseDNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
4938YMR157CAIM36Protein of unknown functionProtein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
4939YMR158C-AYMR158C-APutative protein of unknown functionPutative protein of unknown function; may contain a lipid attachment site; YMR158C-A is not an essential gene
4940YMR158WMRPS8Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
4941YMR158W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C
4942YMR159CATG16Conserved protein involved in autophagyConserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
4943YMR160WYMR160WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
4944YMR161WHLJ1Co-chaperone for Hsp40pCo-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
4945YMR162CDNF3Trans-golgi network aminophospholipid translocase (flippase)Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase
4946YMR163CINP2Peroxisome-specific receptor important for peroxisome inheritancePeroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
4947YMR164CMSS11Transcription factorTranscription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1
4948YMR165CPAH1Mg2+-dependent phosphatidate (PA) phosphataseMg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2
4949YMR166CMME1Transporter of the mitochondrial inner membrane that exports magnesiumPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
4950YMR167WMLH1Protein required for mismatch repair in mitosis and meiosisProtein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
4951YMR168CCEP3Essential kinetochore proteinEssential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
4952YMR169CALD3Cytoplasmic aldehyde dehydrogenaseCytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
4953YMR170CALD2Cytoplasmic aldehyde dehydrogenaseCytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p
4954YMR171CEAR1Specificity factor required for Rsp5p-dependent ubiquitinationSpecificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
4955YMR172C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF HOT1/YMR172W
4956YMR172WHOT1Transcription factor for glycerol biosynthetic genesTranscription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
4957YMR173WDDR48DNA damage-responsive proteinDNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
4958YMR173W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
4959YMR174CPAI3Cytoplasmic proteinase A (Pep4p) inhibitorCytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
4960YMR175WSIP18Phospholipid-binding hydrophilinPhospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication
4961YMR175W-AYMR175W-APutative protein of unknown functionPutative protein of unknown function
4962YMR176WECM5Subunit of the Snt2C complexSubunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
4963YMR177WMMT1Putative metal transporter involved in mitochondrial iron accumulationPutative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication
4964YMR178WYMR178WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress
4965YMR179WSPT21Protein with a role in transcriptional silencingProtein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
4966YMR180CCTL1RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasmRNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; CTL1 has a paralog, CET1, that arose from the whole genome duplication
4967YMR181CYMR181CProtein of unknown functionProtein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication
4968YMR182CRGM1Putative zinc finger DNA binding transcription factorPutative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
4969YMR182W-AMIN3Putative protein of unknown functionPutative protein of unknown function
4970YMR183CSSO2Plasma membrane t-SNAREPlasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication
4971YMR184WADD37Protein of unknown functionProtein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress
4972YMR185WRTP1Protein required for the nuclear import and biogenesis of RNA pol IIProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
4973YMR186WHSC82Cytoplasmic chaperone of the Hsp90 familyCytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
4974YMR187CYMR187CPutative protein of unknown functionPutative protein of unknown function; YMR187C is not an essential gene
4975YMR188CMRPS17Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
4976YMR189WGCV2P subunit of the mitochondrial glycine decarboxylase complexP subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
4977YMR190CSGS1RecQ family nucleolar DNA helicaseRecQ family nucleolar DNA helicase; role in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; stimulates DNA catenation/decatenation activity of Top3p; potential repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
4978YMR191WSPG5Protein required for proteasome assembly during quiescenceProtein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
4979YMR192WGYL1Putative GTPase activating protein (GAP) with a role in exocytosisPutative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication
4980YMR193C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4981YMR193WMRPL24Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
4982YMR194C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
4983YMR194C-BCMC4Protein that localizes to the mitochondrial intermembrane spaceProtein that localizes to the mitochondrial intermembrane space; localizes via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
4984YMR194WRPL36ARibosomal 60S subunit protein L36ARibosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
4985YMR195WICY1Protein of unknown functionProtein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ICY2, that arose from the whole genome duplication
4986YMR196WYMR196WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
4987YMR197CVTI1Protein involved in cis-Golgi membrane trafficProtein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
4988YMR198WCIK1Kinesin-associated proteinKinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
4989YMR199WCLN1G1 cyclin involved in regulation of the cell cycleG1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication
4990YMR200WROT1Molecular chaperone involved in protein folding in ERMolecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface
4991YMR201CRAD14Protein that recognizes and binds damaged DNA during NERProtein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair
4992YMR202WERG2C-8 sterol isomeraseC-8 sterol isomerase; catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
4993YMR203WTOM40Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore; pre-Tom40p is phosphorylated by PKA, which impairs its import into mitochondria under non-respiratory conditions
4994YMR204CINP1Peripheral membrane protein of peroxisomesPeripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane
4995YMR205CPFK2Beta subunit of heterooctameric phosphofructokinaseBeta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
4996YMR206WYMR206WPutative protein of unknown functionPutative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
4997YMR207CHFA1Mitochondrial acetyl-coenzyme A carboxylaseMitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; HFA1 has a paralog, ACC1, that arose from the whole genome duplication
4998YMR208WERG12Mevalonate kinaseMevalonate kinase; acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
4999YMR209CYMR209CPutative S-adenosylmethionine-dependent methyltransferasePutative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene
5000YMR210WMGL2Monoacylglycerol and triacylglycerol lipasePutative acyltransferase with similarity to Eeb1p and Eht1p; has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
5001YMR211WDML1Essential protein involved in mtDNA inheritanceEssential protein involved in mtDNA inheritance; may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
5002YMR212CEFR3Protein required for Stt4-containing PI kinase complex localizationProtein required for Stt4-containing PI kinase complex localization; required for Stt4-containing phosphoinositide (PI) kinase patch assembly at the plasma membrane; recruited to the plasma membrane via a conserved basic patch near its N-terminus; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene
5003YMR213WCEF1Essential splicing factorEssential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
5004YMR214WSCJ1One of several homologs of bacterial chaperone DnaJOne of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
5005YMR215WGAS3Putative 1,3-beta-glucanosyltransferasePutative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
5006YMR216CSKY1SR protein kinase (SRPK)SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
5007YMR217WGUA1GMP synthaseGMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA
5008YMR218CTRS130Component of transport protein particle (TRAPP) complex IIComponent of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
5009YMR219WESC1Protein involved in telomeric silencingProtein localized to the nuclear periphery; involved in telomeric silencing; interacts with PAD4-domain of Sir4p
5010YMR220WERG8Phosphomevalonate kinasePhosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
5011YMR221CFMP42Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
5012YMR222CFSH2Putative serine hydrolase that localizes to the cytoplasmPutative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
5013YMR223WUBP8Ubiquitin-specific protease component of the SAGA acetylation complexUbiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B
5014YMR224CMRE11Nuclease subunit of the MRX complex with Rad50p and Xrs2pNuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
5015YMR225CMRPL44Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
5016YMR226CYMR226CNADP(+)-dependent serine dehydrogenase and carbonyl reductaseNADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
5017YMR227CTAF7TFIID subunit (67 kDa)TFIID subunit (67 kDa); involved in RNA polymerase II transcription initiation
5018YMR228WMTF1Mitochondrial RNA polymerase specificity factorMitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
5019YMR229CRRP5RNA binding protein involved in synthesis of 18S and 5.8S rRNAsRNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
5020YMR230WRPS10BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication
5021YMR230W-AYMR230W-APutative protein of unknown functionPutative protein of unknown function
5022YMR231WPEP5Histone E3 ligase, component of CORVET membrane tethering complexHistone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones
5023YMR232WFUS2Cell fusion regulatorCell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p
5024YMR233WTRI1Non-essential sumoylated protein of unknown functionNon-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
5025YMR234WRNH1Ribonuclease H1Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
5026YMR235CRNA1GTPase activating protein (GAP) for Gsp1pGTPase activating protein (GAP) for Gsp1p; involved in nuclear transport
5027YMR236WTAF9Subunit (17 kDa) of TFIID and SAGA complexesSubunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
5028YMR237WBCH1Member of the ChAPs family (Chs5p-Arf1p-binding proteins)Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication
5029YMR238WDFG5Putative mannosidasePutative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
5030YMR239CRNT1Nuclear dsRNA-specific ribonuclease (RNase III)Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
5031YMR240CCUS1Protein required for assembly of U2 snRNP into the spliceosomeProtein required for assembly of U2 snRNP into the spliceosome; forms a complex with Hsh49p and Hsh155p
5032YMR241WYHM2Citrate and oxoglutarate carrier proteinCitrate and oxoglutarate carrier protein; exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
5033YMR242CRPL20ARibosomal 60S subunit protein L20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
5034YMR242W-AYMR242W-APutative protein of unknown functionPutative protein of unknown function
5035YMR243CZRC1Vacuolar membrane zinc transporterVacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
5036YMR244C-ACOA6Protein involved in cytochrome c oxidase (Complex IV) assemblyProtein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
5037YMR244WYMR244WPutative protein of unknown functionPutative protein of unknown function
5038YMR245WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5039YMR246WFAA4Long chain fatty acyl-CoA synthetaseLong chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
5040YMR247CRKR1RING domain E3 ubiquitin ligaseRING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins; component of ribosome-bound RQC (ribosome quality control) complex required for degradation of polypeptides arising from stalled translation; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration
5041YMR247W-AYMR247W-APutative protein of unknown functionPutative protein of unknown function
5042YMR250WGAD1Glutamate decarboxylaseGlutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
5043YMR251WGTO3Omega class glutathione transferaseOmega class glutathione transferase; putative cytosolic localization
5044YMR251W-AHOR7Protein of unknown functionProtein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
5045YMR252CMLO1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene
5046YMR253CYMR253CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
5047YMR254CYMR254CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5048YMR255WGFD1Coiled-coiled protein of unknown functionCoiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
5049YMR256CCOX7Subunit VII of cytochrome c oxidase (Complex IV)Subunit VII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
5050YMR257CPET111Mitochondrial translational activator specific for the COX2 mRNAMitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
5051YMR258CROY1GTPase inhibitor with similarity to F-box proteinsGTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
5052YMR259CTRM732Protein involved in 2'-O-methylation of C32 of substrate tRNAsInteracts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene
5053YMR260CTIF11Translation initiation factor eIF1ATranslation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
5054YMR261CTPS3Regulatory subunit of trehalose-6-phosphate synthase/phosphataseRegulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
5055YMR262WYMR262WProtein of unknown functionProtein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
5056YMR263WSAP30Component of Rpd3L histone deacetylase complexComponent of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
5057YMR264WCUE1Ubiquitin-binding proteinUbiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
5058YMR265CYMR265CPutative protein of unknown functionPutative protein of unknown function
5059YMR266WRSN1Membrane protein of unknown functionMembrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
5060YMR267WPPA2Mitochondrial inorganic pyrophosphataseMitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate
5061YMR268CPRP24Splicing factor that reanneals snRNPs during spliceosome recyclingSplicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs
5062YMR269WTMA23Nucleolar protein implicated in ribosome biogenesisNucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
5063YMR270CRRN9Protein involved in promoting high level transcription of rDNAProtein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
5064YMR271CURA10Minor orotate phosphoribosyltransferase (OPRTase) isozymeMinor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
5065YMR272CSCS7Sphingolipid alpha-hydroxylaseSphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
5066YMR272W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SCS7/YMR272C
5067YMR272W-BYMR272W-BProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5068YMR273CZDS1Protein with a role in regulating Swe1p-dependent polarized growthProtein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
5069YMR274CRCE1Type II CAAX prenyl proteaseType II CAAX prenyl protease; involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
5070YMR275CBUL1Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complexUbiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
5071YMR276WDSK2Nuclear-enriched ubiquitin-like polyubiquitin-binding proteinNuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
5072YMR277WFCP1Carboxy-terminal domain (CTD) phosphataseCarboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia
5073YMR278WPRM15PhosphoribomutasePhosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
5074YMR279CYMR279CPutative boron transporter involved in boron efflux and resistancePutative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
5075YMR280CCAT8Zinc cluster transcriptional activatorZinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
5076YMR281WGPI12ER membrane protein involved in the second step of GPI anchor assemblyER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol
5077YMR282CAEP2Mitochondrial proteinMitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
5078YMR283CRIT1Initiator methionine 2'-O-ribosyl phosphate transferaseInitiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
5079YMR284WYKU70Subunit of the telomeric Ku complex (Yku70p-Yku80p)Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
5080YMR285CNGL2Protein involved in 5.8S rRNA processingProtein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
5081YMR286WMRPL33Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
5082YMR287CDSS13'-5' exoribonuclease3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
5083YMR288WHSH155U2-snRNP associated splicing factorU2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
5084YMR289WABZ2Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase)Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase); catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
5085YMR290CHAS1ATP-dependent RNA helicaseATP-dependent RNA helicase; involved in the biogenesis of 40S and 60S ribosome subunits; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
5086YMR290W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1/YMR290C gene which encodes an ATP-dependent RNA helicase
5087YMR291WTDA1Protein kinase of unknown cellular roleProtein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
5088YMR292WGOT1Homodimeric protein that is packaged into COPII vesiclesHomodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
5089YMR293CHER2Subunit of the trimeric GatFAB AmidoTransferase(AdT) complexSubunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
5090YMR294WJNM1Component of the yeast dynactin complexComponent of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
5091YMR294W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
5092YMR295CYMR295CProtein of unknown function that associates with ribosomesProtein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
5093YMR296CLCB1Component of serine palmitoyltransferaseComponent of serine palmitoyltransferase; responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
5094YMR297WPRC1Vacuolar carboxypeptidase Y (proteinase C, CPY)Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family
5095YMR298WLIP1Ceramide synthase subunitCeramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
5096YMR299CDYN3Dynein light intermediate chain (LIC)Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
5097YMR300CADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT)Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase
5098YMR301CATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporterMitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
5099YMR302CYME2Integral inner mitochondrial membrane proteinIntegral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
5100YMR303CADH2Glucose-repressible alcohol dehydrogenase IIGlucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
5101YMR304C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 3' end of verified ORF SCW10/YMR305C
5102YMR304WUBP15Ubiquitin-specific protease involved in protein deubiquitinationUbiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p
5103YMR305CSCW10Cell wall protein with similarity to glucanasesCell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication
5104YMR306C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF FKS3/YMR306W
5105YMR306WFKS3Protein involved in spore wall assemblyProtein involved in spore wall assembly; has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5106YMR307C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GAS1/YMR307W
5107YMR307WGAS1Beta-1,3-glucanosyltransferaseBeta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation
5108YMR308CPSE1Karyopherin/importin that interacts with the nuclear pore complexKaryopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
5109YMR309CNIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3)eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection
5110YMR310CYMR310CPutative methyltransferasePutative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication
5111YMR311CGLC8Regulatory subunit of protein phosphatase 1 (Glc7p)Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
5112YMR312WELP6Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplexSubunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
5113YMR313CTGL3Bifunctional triacylglycerol lipase and LPE acyltransferaseBifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation
5114YMR314WPRE5Alpha 6 subunit of the 20S proteasomeAlpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
5115YMR315WYMR315WProtein with NADP(H) oxidoreductase activityProtein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
5116YMR315W-AYMR315W-APutative protein of unknown functionPutative protein of unknown function
5117YMR316C-AYMR316C-AProtein of unknown functionProtein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W
5118YMR316C-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 5' end of ORF YMR317W
5119YMR316WDIA1Protein of unknown functionProtein of unknown function; involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
5120YMR317WYMR317WPutative protein of unknown functionPutative protein of unknown function; has some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene
5121YMR318CADH6NADPH-dependent medium chain alcohol dehydrogenaseNADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
5122YMR319CFET4Low-affinity Fe(II) transporter of the plasma membraneLow-affinity Fe(II) transporter of the plasma membrane
5123YMR320WYMR320WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5124YMR321CYMR321CPutative protein of unknown functionPutative protein of unknown function; proposed to be a palmitoylated membrane protein; YMR321C has a paralog, SAM4, that arose from a single-locus duplication
5125YMR322CSNO4Possible chaperone and cysteine proteasePossible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
5126YMR323WERR3Enolase, a phosphopyruvate hydrataseEnolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant in glucose
5127YMR324CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription is AZF1 dependent in glycerol-lactate medium and SLT2 dependent in response to the lipid hydroperoxides
5128YMR325WPAU19Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
5129YMR326CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13
5130YNL001WDOM34Protein that facilitates ribosomal subunit dissociationProtein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
5131YNL002CRLP7Nucleolar protein similar to large ribosomal subunit L7 proteinsNucleolar protein similar to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs; binds junction of ITS2 and ITS2-proximal stem between the 3' end of 5.8S rRNA and the 5' end of 25S rRNA
5132YNL003CPET8S-adenosylmethionine transporter of the mitochondrial inner membraneS-adenosylmethionine transporter of the mitochondrial inner membrane; member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth
5133YNL004WHRB1Poly(A+) RNA-binding proteinPoly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
5134YNL005CMRP7Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
5135YNL006WLST8Protein required for the transport of Gap1pProtein required for the transport of Gap1p; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
5136YNL007CSIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1pType II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1
5137YNL008CASI3Subunit of the inner nuclear membrane Asi ubiquitin ligase complexSubunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI3 has a paralog, ASI1, that arose from the whole genome duplication
5138YNL009WIDP3Peroxisomal NADP-dependent isocitrate dehydrogenasePeroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
5139YNL010WYNL010WPutative protein of unknown functionPutative protein of unknown function; similar to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation
5140YNL011CYNL011CPutative protein of unknown functionPutative protein of unknown function; YNL011C is not an essential gene
5141YNL012WSPO1Meiosis-specific prospore proteinMeiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
5142YNL013CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W
5143YNL014WHEF3Translational elongation factor EF-3Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication
5144YNL015WPBI2Cytosolic inhibitor of vacuolar proteinase B (PRB1)Cytosolic inhibitor of vacuolar proteinase B (PRB1); required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion; protein abundance increases in response to DNA replication stress
5145YNL016WPUB1Poly (A)+ RNA-binding proteinPoly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
5146YNL017CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the tRNA ORF tI(AAU)N2
5147YNL018CYNL018CPutative protein of unknown functionPutative protein of unknown function; YNL018C has a paralog, YNL034W, that arose from a segmental duplication
5148YNL019CYNL019CPutative protein of unknown functionPutative protein of unknown function; expression induced during heat stress; YNL019C has a paralog, YNL033W, that arose from a segmental duplication
5149YNL020CARK1Serine/threonine protein kinaseSerine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication
5150YNL021WHDA1Putative catalytic subunit of a class II histone deacetylase complexPutative catalytic subunit of a class II histone deacetylase complex; role in azole resistance via Hsp90p, and in the heat shock response; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; other members of the HDA1 histone deacetylase complex are Hda2p and Hda3p
5151YNL022CRCM1rRNA m5C methyltransferaserRNA m5C methyltransferase; methylates cytosine at position 2278 of 25S rRNA while Nop2p methylates cytosine at position 2870; contains seven beta-strand methyltransferase motif; localized to the nucleolus; interacts with Trm112p; homolog of NSUN5A, a human gene which is deleted in Williams-Beuren Syndrome
5152YNL023CFAP1Protein that binds to Fpr1pProtein that binds to Fpr1p; confers rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1
5153YNL024CEFM6Putative S-adenosylmethionine-dependent lysine methyltransferasePutative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern
5154YNL024C-AKSH1Essential protein suggested to function early in the secretory pathwayEssential protein suggested to function early in the secretory pathway; inviability is suppressed by overexpression of Golgi protein Tvp23p; ortholog of human Kish
5155YNL025CSSN8Cyclin-like component of the RNA polymerase II holoenzymeCyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C
5156YNL026WSAM50Component of the Sorting and Assembly Machinery (SAM) complexComponent of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
5157YNL027WCRZ1Transcription factor, activates transcription of stress response genesTranscription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
5158YNL028WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5159YNL029CKTR5Putative mannosyltransferase involved in protein glycosylationPutative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR5 has a paralog, KTR7, that arose from the whole genome duplication
5160YNL030WHHF2Histone H4Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
5161YNL031CHHT2Histone H3Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
5162YNL032WSIW14Tyrosine phosphatase involved in actin organization and endocytosisTyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm
5163YNL033WYNL033WPutative protein of unknown functionPutative protein of unknown function; YNL033W has a paralog, YNL019C, that arose from a segmental duplication
5164YNL034WYNL034WPutative protein of unknown functionPutative protein of unknown function; not an essential gene; YNL034W has a paralog, YNL018C, that arose from a segmental duplication
5165YNL035CYNL035CNuclear protein of unknown functionNuclear protein of unknown function; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress
5166YNL036WNCE103Carbonic anhydraseCarbonic anhydrase; metalloenzyme that catalyzes CO2 hydration to bicarbonate, which is an important metabolic substrate, and protons; not expressed under conditions of high CO2, such as inside a growing colony, but transcription is induced in response to low CO2 levels, such as on the colony surface in ambient air; poorly transcribed under aerobic conditions and at an undetectable level under anaerobic conditions; abundance increases in response to DNA replication stress
5167YNL037CIDH1Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenaseSubunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
5168YNL038WGPI15Protein involved in the synthesis of GlcNAc-PIProtein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
5169YNL039WBDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB)Essential subunit of RNA polymerase III transcription factor (TFIIIB); TFIIIB is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
5170YNL040WYNL040WProtein of unknown functionPutative protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene
5171YNL041CCOG6Component of the conserved oligomeric Golgi complexComponent of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
5172YNL042WBOP3Protein of unknown functionProtein of unknown function; potential Cdc28p substrate; overproduction confers resistance to methylmercury
5173YNL042W-BYNL042W-BPutative protein of unknown functionPutative protein of unknown function
5174YNL043CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
5175YNL044WYIP3Protein localized to COPII vesiclesProtein localized to COPII vesicles; proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
5176YNL045WLAP2Leucyl aminopeptidase yscIV with epoxide hydrolase activityLeucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
5177YNL046WYNL046WPutative protein of unknown functionPutative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
5178YNL047CSLM2Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1pPhosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
5179YNL048WALG11Alpha-1,2-mannosyltransferaseAlpha-1,2-mannosyltransferase; catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER
5180YNL049CSFB2Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coatComponent of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication
5181YNL050CYNL050CPutative protein of unknown functionPutative protein of unknown function; YNL050c is not an essential gene
5182YNL051WCOG5Component of the conserved oligomeric Golgi complexComponent of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
5183YNL052WCOX5ASubunit Va of cytochrome c oxidaseSubunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
5184YNL053WMSG5Dual-specificity protein phosphataseDual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication
5185YNL054WVAC7Integral vacuolar membrane proteinIntegral vacuolar membrane protein; involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock
5186YNL054W-AYNL054W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
5187YNL055CPOR1Mitochondrial porin (voltage-dependent anion channel)Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
5188YNL056WOCA2Protein of unknown functionPutative protein of unknown function; similar to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
5189YNL057WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5190YNL058CYNL058CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuole; not an essential gene; YNL058C has a paralog, PRM5, that arose from the whole genome duplication
5191YNL059CARP5Nuclear actin-related protein involved in chromatin remodelingNuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; promotes nucleosome shifts in the 3 prime direction
5192YNL061WNOP2rRNA m5C methyltransferaserRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA while Rcm1p methylates cytosine at position 2278; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; homolog of p120/NSUN1, a human gene upregulated in cancer
5193YNL062CGCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14pSubunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
5194YNL063WMTQ1S-adenosylmethionine-dependent methyltransferaseS-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
5195YNL064CYDJ1Type I HSP40 co-chaperoneType I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family
5196YNL065WAQR1Plasma membrane transporter of the major facilitator superfamilyPlasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
5197YNL066WSUN4Cell wall protein related to glucanasesCell wall protein related to glucanases; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication
5198YNL067WRPL9BRibosomal 60S subunit protein L9BRibosomal 60S subunit protein L9B; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9B has a paralog, RPL9A, that arose from a single-locus duplication
5199YNL067W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5200YNL067W-BYNL067W-BPutative protein of unknown functionPutative protein of unknown function
5201YNL068CFKH2Forkhead family transcription factorForkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; facilitates clustering and activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; relocalizes to the cytosol in response to hypoxia; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
5202YNL069CRPL16BRibosomal 60S subunit protein L16BRibosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication
5203YNL070WTOM7Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
5204YNL071WLAT1Dihydrolipoamide acetyltransferase component (E2) of the PDCDihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
5205YNL072WRNH201Ribonuclease H2 catalytic subunitRibonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
5206YNL073WMSK1Mitochondrial lysine-tRNA synthetaseMitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)
5207YNL074CMLF3Serine-rich protein of unknown functionSerine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication
5208YNL075WIMP4Component of the SSU processomeComponent of the SSU processome; SSU processome is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
5209YNL076WMKS1Pleiotropic negative transcriptional regulatorPleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
5210YNL077WAPJ1Chaperone with a role in SUMO-mediated protein degradationChaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
5211YNL078WNIS1Protein localized in the bud neck at G2/M phaseProtein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network
5212YNL079CTPM1Major isoform of tropomyosinMajor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
5213YNL080CEOS1Protein involved in N-glycosylationProtein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene
5214YNL081CSWS2Putative mitochondrial ribosomal protein of the small subunitPutative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2
5215YNL082WPMS1ATP-binding protein required for mismatch repairATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
5216YNL083WSAL1ADP/ATP transporterADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
5217YNL084CEND3EH domain-containing protein involved in endocytosisEH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
5218YNL085WMKT1Protein similar to nucleases that forms a complex with Pbp1pProtein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains
5219YNL086WSNN1Subunit of the BLOC-1 complex involved in endosomal maturationSubunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes
5220YNL087WTCB2ER protein involved in ER-plasma membrane tetheringER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication
5221YNL088WTOP2Topoisomerase IITopoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
5222YNL089CDubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins
5223YNL090WRHO2Non-essential small GTPase of the Rho/Rac family of Ras-like proteinsNon-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity and in microtubule assembly
5224YNL091WNST1Protein of unknown functionProtein of unknown function; mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
5225YNL092WYNL092WS-adenosylmethionine-dependent protein methyltransferasePutative S-adenosylmethionine-dependent methyltransferase; of the seven beta-strand family; YNL092W is not an essential gene
5226YNL093WYPT53Stress-induced Rab family GTPaseStress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
5227YNL094WAPP1Phosphatidate phosphatase, converts phosphatidate to diacylglycerolPhosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
5228YNL095CYNL095CPutative protein of unknown functionPutative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
5229YNL096CRPS7BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
5230YNL097CPHO23Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
5231YNL097C-BYNL097C-BPutative protein of unknown functionPutative protein of unknown function
5232YNL097W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene PHO23/YNL097C
5233YNL098CRAS2GTP-binding proteinGTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
5234YNL099COCA1Putative protein tyrosine phosphatasePutative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
5235YNL100WMIC27Component of the MICOS complexComponent of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane
5236YNL101WAVT4Vacuolar transporterVacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
5237YNL102WPOL1Catalytic subunit of the DNA polymerase I alpha-primase complexCatalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
5238YNL103WMET4Leucine-zipper transcriptional activatorLeucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p
5239YNL103W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MET4/YNL104C
5240YNL104CLEU4Alpha-isopropylmalate synthase (2-isopropylmalate synthase)Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
5241YNL105WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
5242YNL106CINP52Polyphosphatidylinositol phosphatasePolyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication
5243YNL107WYAF9Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexesSubunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain
5244YNL108CYNL108CProtein phosphataseProtein phosphatase; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
5245YNL109WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C
5246YNL110CNOP15Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
5247YNL111CCYB5Cytochrome b5Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
5248YNL112WDBP2ATP-dependent RNA helicase of the DEAD-box protein familyATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; interacts with YRA1; required for the assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
5249YNL113WRPC19RNA polymerase subunit AC19RNA polymerase subunit AC19; common to RNA polymerases I and III
5250YNL114CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
5251YNL115CYNL115CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
5252YNL116WDMA2Ubiquitin-protein ligase (E3)Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
5253YNL117WMLS1Malate synthase, enzyme of the glyoxylate cycleMalate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA
5254YNL118CDCP2Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complexCatalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; also enters the nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
5255YNL119WNCS2Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAsProtein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
5256YNL120CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
5257YNL121CTOM70Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
5258YNL122CYNL122CMitochondrial ribosomal protein of the large subunitPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene
5259YNL123WNMA111Serine protease and general molecular chaperoneSerine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
5260YNL124WNAF1RNA-binding protein required for the assembly of box H/ACA snoRNPsRNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
5261YNL125CESBP6Protein with similarity to monocarboxylate permeasesProtein with similarity to monocarboxylate permeases; appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
5262YNL126WSPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complexComponent of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
5263YNL127WFAR11Protein involved in recovery from cell cycle arrestProtein involved in recovery from cell cycle arrest; acts in response to pheromone; also involved in regulation of intra-S DNA damage checkpoint and autophagy; is essential for dephosphorylation of Atg13p; interacts with Far3p, Far7p, Far8p, Far9p, Far10p and with the phosphatases Pph21p, Pph22p and Pph3p; has similarity to the N- and C-termini of N. crassa HAM-2; similar to human Fam40A and Fam40B
5264YNL128WTEP1PTEN homolog with no demonstrated inositol lipid phosphatase activityPTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1
5265YNL129WNRK1Nicotinamide riboside kinaseNicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
5266YNL130CCPT1CholinephosphotransferaseCholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
5267YNL130C-ADGR1Protein of unknown functionProtein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose
5268YNL131WTOM22Component of the TOM (Translocase of Outer Membrane) complexComponent of the TOM (Translocase of Outer Membrane) complex; responsible for initial import of mitochondrially directed proteins; mediates interaction between TOM and TIM complexes and acts as a receptor for precursor proteins
5269YNL132WKRE33Protein required for biogenesis of the small ribosomal subunitProtein required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance; essential gene; NAT10, the human homolog, implicated in several types of cancer and premature aging.
5270YNL133CFYV6Protein of unknown functionProtein of unknown function; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
5271YNL134CYNL134CNADH-dependent aldehyde reductase, involved in detoxification of furfProtein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
5272YNL135CFPR1Peptidyl-prolyl cis-trans isomerase (PPIase)Peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; N-terminally propionylated in vivo
5273YNL136WEAF7Subunit of the NuA4 histone acetyltransferase complexSubunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A
5274YNL137CNAM9Mitochondrial ribosomal component of the small subunitMitochondrial ribosomal component of the small subunit
5275YNL138WSRV2CAP (cyclase-associated protein)CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus, in physically and genetically separate activity, binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis
5276YNL138W-AYSF3Component of the SF3b subcomplex of the U2 snRNPComponent of the SF3b subcomplex of the U2 snRNP; essential protein required for for splicing and for assembly of SF3b
5277YNL139CTHO2Subunit of the THO complexSubunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
5278YNL140CYNL140CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
5279YNL141WAAH1Adenine deaminase (adenine aminohydrolase)Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
5280YNL142WMEP2Ammonium permease involved in regulation of pseudohyphal growthAmmonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
5281YNL143CYNL143CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
5282YNL144CYNL144CPutative protein of unknown functionPutative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; YNL144C has a paralog, YHR131C, that arose from the whole genome duplication
5283YNL144W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YNL144C
5284YNL145WMFA2Mating pheromone a-factorMating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
5285YNL146C-AYNL146C-APutative protein of unknown functionPutative protein of unknown function
5286YNL146WYNL146WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
5287YNL147WLSM7Lsm (Like Sm) proteinLsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
5288YNL148CALF1Alpha-tubulin folding proteinAlpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
5289YNL149CPGA2Essential protein required for maturation of Gas1p and Pho8pEssential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
5290YNL150WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
5291YNL151CRPC31RNA polymerase III subunit C31RNA polymerase III subunit C31
5292YNL152WINN1Essential protein that associates with contractile actomyosin ringEssential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
5293YNL153CGIM3Subunit of the heterohexameric cochaperone prefoldin complexSubunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
5294YNL154CYCK2Palmitoylated plasma membrane-bound casein kinase I (CK1) isoformPalmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK2 has a paralog, YCK1, that arose from the whole genome duplication
5295YNL155WCUZ1Protein with a role in the ubiquitin-proteasome pathwayProtein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress
5296YNL156CNSG2Protein involved in regulation of sterol biosynthesisProtein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
5297YNL157WIGO1Protein required for initiation of G0 programProtein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication
5298YNL158WPGA1Essential component of GPI-mannosyltransferase IIEssential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes
5299YNL159CASI2Subunit of the inner nuclear membrane Asi ubiquitin ligase complexSubunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids
5300YNL160WYGP1Cell wall-related secretory glycoproteinCell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; YGP1 has a paralog, SPS100, that arose from the whole genome duplication
5301YNL161WCBK1Serine/threonine protein kinase of the the RAM signaling networkSerine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
5302YNL162WRPL42ARibosomal 60S subunit protein L42ARibosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication
5303YNL162W-AYNL162W-APutative protein of unknown functionPutative protein of unknown function; identified by homology
5304YNL163CRIA1Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesisCytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
5305YNL164CIBD2Component of the BUB2-dependent spindle checkpoint pathwayComponent of the BUB2-dependent spindle checkpoint pathway; interacts with Bfa1p and functions upstream of Bub2p and Bfa1p
5306YNL165WYNL165WPutative protein of unknown functionPutative protein of unknown function; YNL165W is not an essential gene
5307YNL166CBNI5Linker protein responsible for recruitment of myosin to the bud neckProtein involved in organization of septins at the mother-bud neck; may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
5308YNL167CSKO1Basic leucine zipper transcription factor of the ATF/CREB familyBasic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses
5309YNL168CFMP41Putative protein of unknown functionPutative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5310YNL169CPSD1Phosphatidylserine decarboxylase of the mitochondrial inner membranePhosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology by affecting lipid mixing in the mitochondrial membrane and by influencing the ratio of long to short forms of Mgm1p; partly exposed to the mitochondrial intermembrane space
5311YNL170WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5312YNL171CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5313YNL172WAPC1Largest subunit of the Anaphase-Promoting Complex/CyclosomeLargest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
5314YNL173CMDG1Plasma membrane proteinPlasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication
5315YNL174WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NOP13/YNL175C
5316YNL175CNOP13Nucleolar protein found in preribosomal complexesNucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
5317YNL176CTDA7Cell cycle-regulated gene of unknown functionCell cycle-regulated gene of unknown function; promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele; TDA7 has a paralog, YDL211C, that arose from the whole genome duplication
5318YNL177CMRPL22Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
5319YNL178WRPS3Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
5320YNL179CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
5321YNL180CRHO5Non-essential small GTPase of the Rho/Rac family of Ras-like proteinsNon-essential small GTPase of the Rho/Rac family of Ras-like proteins; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity
5322YNL181WPBR1Putative oxidoreductasePutative oxidoreductase; required for cell viability
5323YNL182CIPI3Component of the Rix1 complex and pre-replicative complexes (pre-RCs)Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p
5324YNL183CNPR1Protein kinaseProtein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex; NPR1 has a paralog, PRR2, that arose from the whole genome duplication
5325YNL184CYNL184CProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
5326YNL185CMRPL19Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
5327YNL186WUBP10Ubiquitin-specific protease, deubiquitinates Ub-protein moietiesUbiquitin-specific protease, deubiquitinates Ub-protein moieties; interacts with proteins that function in rRNA production and ribosome biogenesis; stabilizes Rpa190p, the largest subunit of RNAP I, by mediating its deubiquitination; controls PCNA deubiquitylation; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; localized to the nucleolus; human USP36 is a functional analog of Ubp10p
5328YNL187WSWT21Protein involved in mRNA splicingProtein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats
5329YNL188WKAR1Protein involved in karyogamy and spindle pole body duplicationProtein involved in karyogamy and spindle pole body duplication; involved in karyogamy during mating; involved in spindle pole body duplication during mitosis; localizes to the half-bridge of the spindle pole body; interacts with Spc72p during karyogamy; also interacts with Cdc31p; essential gene
5330YNL189WSRP1Karyopherin alpha homologKaryopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation
5331YNL190WYNL190WHydrophilin essential in desiccation-rehydration processHydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site
5332YNL191WDUG3Component of glutamine amidotransferase (GATase II)Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
5333YNL192WCHS1Chitin synthase IChitin synthase I; requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor
5334YNL193WYNL193WPutative protein of unknown functionPutative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
5335YNL194CYNL194CIntegral membrane proteinIntegral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication
5336YNL195CYNL195CProtein of unknown functionProtein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YNL195C has a paralog, HBT1, that arose from the whole genome duplication
5337YNL196CSLZ1Sporulation-specific protein with a leucine zipper motifSporulation-specific protein with a leucine zipper motif; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation
5338YNL197CWHI3RNA binding protein that sequesters CLN3 mRNA in cytoplasmic fociRNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; regulates genes involved in the cell cycle, sister chromatid cohesion, and stress response; acts as a cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; Tpk1p (PKA) mediated phosphorylation (S568) inhibits Whi3p function, decreasing its interaction with CLN3 mRNA; regulates ploidy
5339YNL198CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5340YNL199CGCR2Transcriptional activator of genes involved in glycolysisTranscriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p
5341YNL200CNNR1NADHX epimeraseNADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5342YNL201CPSY2Subunit of protein phosphatase PP4 complexSubunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
5343YNL202WSPS19Peroxisomal 2,4-dienoyl-CoA reductasePeroxisomal 2,4-dienoyl-CoA reductase; auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
5344YNL203CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5345YNL204CSPS18Protein of unknown function, contains a putative zinc-binding domainProtein of unknown function, contains a putative zinc-binding domain; expressed during sporulation; SPS18 has a paralog, GCS1, that arose from the whole genome duplication
5346YNL205CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5347YNL206CRTT106Histone chaperoneHistone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1)
5348YNL207WRIO2Essential serine kinase involved in the processing of 20S pre-rRNAEssential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
5349YNL208WYNL208WProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi
5350YNL209WSSB2Cytoplasmic ATPase that is a ribosome-associated molecular chaperoneCytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
5351YNL210WMER1mRNA-binding protein required for meiosis-specific mRNA splicingmRNA-binding protein required for meiosis-specific mRNA splicing; required for chromosome pairing and meiotic recombination; Mer1p regulon embraces four essential meiotic pre-mRNAs: REC107, HFM1, AMA1 and SPO22
5352YNL211CMRX7Protein that associates with mitochondrial ribosomePutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
5353YNL212WVID27Cytoplasmic protein of unknown functionCytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
5354YNL213CRRG9Protein of unknown functionProtein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5355YNL214WPEX17Membrane peroxin of the peroxisomal importomer complexMembrane peroxin of the peroxisomal importomer complex; complex facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
5356YNL215WIES2Protein that associates with the INO80 chromatin remodeling complexProtein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
5357YNL216WRAP1Essential DNA-binding transcription regulator that binds many lociEssential DNA-binding transcription regulator that binds many loci; involved in transcription activation and repression, chromatin silencing, and telomere length maintenance; relocalizes to the cytosol in response to hypoxia; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
5358YNL217WPPN2Zn2+-dependent endopolyphosphatasePutative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
5359YNL218WMGS1Protein with DNA-dependent ATPase and ssDNA annealing activitiesProtein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
5360YNL219CALG9Mannosyltransferase, involved in N-linked glycosylationMannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
5361YNL220WADE12Adenylosuccinate synthaseAdenylosuccinate synthase; catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
5362YNL221CPOP1Subunit of RNase MRP, nuclear RNase P and telomerase complexesSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
5363YNL222WSSU72Phosphatase and transcription/RNA-processing factorPhosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
5364YNL223WATG4Conserved cysteine protease required for autophagyConserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation
5365YNL224CSQS1Protein that stimulates the ATPase and helicase activities of Prp43pProtein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
5366YNL225CCNM67Component of the spindle pole body outer plaqueComponent of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
5367YNL226WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
5368YNL227CJJJ1Co-chaperone that stimulates the ATPase activity of Ssa1pCo-chaperone that stimulates the ATPase activity of Ssa1p; required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
5369YNL228WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1
5370YNL229CURE2Nitrogen catabolite repression transcriptional regulatorNitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
5371YNL230CELA1Elongin AElongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
5372YNL231CPDR16Phosphatidylinositol transfer protein (PITP)Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
5373YNL232WCSL4Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
5374YNL233WBNI4Targeting subunit for Glc7p protein phosphataseTargeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
5375YNL234WYNL234WProtein of unknown function with similarity to globinsProtein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1
5376YNL235CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
5377YNL236WSIN4Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
5378YNL237WYTP1Probable type-III integral membrane protein of unknown functionProbable type-III integral membrane protein of unknown function; has regions of similarity to mitochondrial electron transport proteins
5379YNL238WKEX2Kexin, a subtilisin-like protease (proprotein convertase)Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
5380YNL239WLAP3Cysteine aminopeptidase with homocysteine-thiolactonase activityCysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
5381YNL240CNAR1Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinerySubunit of the cytosolic iron-sulfur (FeS) protein assembly machinery; required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; deficiency results in shortened lifespan and sensitivity to paraquat; homologous to human Narf
5382YNL241CZWF1Glucose-6-phosphate dehydrogenase (G6PD)Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
5383YNL242WATG2Peripheral membrane protein required for autophagic vesicle formationPeripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress
5384YNL243WSLA2Adaptor protein that links actin to clathrin and endocytosisAdaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo
5385YNL244CSUI1Translation initiation factor eIF1Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
5386YNL245CCWC25Splicing factor required for the first step of pre-mRNA splicingSplicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
5387YNL246WVPS75NAP family histone chaperoneNAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
5388YNL247WYNL247WCysteinyl-tRNA synthetaseCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
5389YNL248CRPA49RNA polymerase I subunit A49RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p
5390YNL249CMPA43Protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5391YNL250WRAD50Subunit of MRX complex with Mre11p and Xrs2pSubunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
5392YNL251CNRD1RNA-binding subunit of Nrd1 complexRNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
5393YNL252CMRPL17Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
5394YNL253WTEX1Protein involved in mRNA exportProtein involved in mRNA export; component of the transcription export (TREX) complex
5395YNL254CRTC4Protein of unknown functionProtein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
5396YNL255CGIS2Translational activator for mRNAs with internal ribosome entry sitesTranslational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
5397YNL256WFOL1Multifunctional enzyme of the folic acid biosynthesis pathwayMultifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
5398YNL257CSIP3Putative sterol transfer proteinTranscription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
5399YNL258CDSL1Peripheral membrane protein needed for Golgi-to-ER retrograde trafficPeripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
5400YNL259CATX1Cytosolic copper metallochaperoneCytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
5401YNL260CLTO1Essential protein that forms a complex with Rli1p and Yae1pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
5402YNL261WORC5Subunit of the origin recognition complex (ORC)Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
5403YNL262WPOL2Catalytic subunit of DNA polymerase (II) epsilonCatalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
5404YNL263CYIF1Integral membrane proteinIntegral membrane protein; required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
5405YNL264CPDR17Phosphatidylinositol transfer protein (PITP)Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition
5406YNL265CIST1Protein with positive role in the multivesicular body sorting pathwayProtein with positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
5407YNL266WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C
5408YNL267WPIK1Phosphatidylinositol 4-kinasePhosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p
5409YNL268WLYP1Lysine permeaseLysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
5410YNL269WBSC4Protein of unknown functionProtein of unknown function; protein-coding gene that evolved de novo via a series of point mutations in noncoding sequence; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; may be involved in DNA repair pathway during stationary phase and contribute to robustness of cells when shifted to a nutrient-poor environment
5411YNL270CALP1Arginine transporterArginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
5412YNL271CBNI1ForminFormin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
5413YNL272CSEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4pGuanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
5414YNL273WTOF1Subunit of a replication-pausing checkpoint complexSubunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia
5415YNL274CGOR1Glyoxylate reductaseGlyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
5416YNL275WBOR1Boron efflux transporter of the plasma membraneBoron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
5417YNL276CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W
5418YNL277WMET2L-homoserine-O-acetyltransferaseL-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
5419YNL277W-AYNL277W-APutative protein of unknown functionPutative protein of unknown function
5420YNL278WCAF120Part of the CCR4-NOT transcriptional regulatory complexPart of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication
5421YNL279WPRM1Pheromone-regulated multispanning membrane proteinPheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p
5422YNL280CERG24C-14 sterol reductaseC-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions
5423YNL281WHCH1Heat shock protein regulatorHeat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
5424YNL282WPOP3Subunit of both RNase MRP and nuclear RNase PSubunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
5425YNL283CWSC2Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathwaySensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication
5426YNL284CMRPL10Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
5427YNL284C-AYNL284C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
5428YNL284C-BYNL284C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
5429YNL285WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5430YNL286WCUS2Putative checkpoint factor in transcriptionPutative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs)
5431YNL287WSEC21Gamma subunit of coatomerGamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
5432YNL288WCAF40Component of the CCR4-NOT transcriptional complexComponent of the CCR4-NOT transcriptional complex; evolutionarily conserved; involved in controlling mRNA initiation, elongation, and degradation; binds Cdc39p
5433YNL289WPCL1Cyclin, interacts with cyclin-dependent kinase Pho85pCyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
5434YNL290WRFC3Subunit of heteropentameric Replication factor C (RF-C)Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
5435YNL291CMID1N-glycosylated integral membrane protein of the ER and plasma membraneN-glycosylated integral membrane protein of the ER and plasma membrane; functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
5436YNL292WPUS4Pseudouridine synthasePseudouridine synthase; catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; also responsible for pseudouracil modification of some mRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
5437YNL293WMSB3Rab GTPase-activating proteinRab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; required for proper actin organization; also localizes to plasma membrane and sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to the TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication
5438YNL294CRIM21pH sensor molecule, component of the RIM101 pathwaypH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
5439YNL295WMRX6Protein that associates with mitochondrial ribosomePutative protein of unknown function
5440YNL296WDubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
5441YNL297CMON2Protein with a role in endocytosis and vacuole integrityProtein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins
5442YNL298WCLA4Cdc42p-activated signal transducing kinaseCdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
5443YNL299WTRF5Non-canonical poly(A) polymeraseNon-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
5444YNL300WTOS6Glycosylphosphatidylinositol-dependent cell wall proteinGlycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
5445YNL301CRPL18BRibosomal 60S subunit protein L18BRibosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
5446YNL302CRPS19BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
5447YNL303WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5448YNL304WYPT11Rab GTPaseRab GTPase; Myo2p-binding protein implicated in mother-to-bud transport of cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during cell division; function is regulated at multiple levels; abundance of active Ypt11p forms is controlled by phosphorylation status and degradation; normally a low-abundance protein whose ER localization is only detected when protein is highly over expressed
5449YNL305CBXI1Protein involved in apoptosisProtein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
5450YNL306WMRPS18Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
5451YNL307CMCK1Dual-specificity ser/thr and tyrosine protein kinaseDual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication
5452YNL308CKRI1Essential nucleolar protein required for 40S ribosome biogenesisEssential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
5453YNL309WSTB1Protein with role in regulation of MBF-specific transcription at StartProtein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication
5454YNL310CZIM17Protein co-chaperone with a zinc finger motifProtein co-chaperone with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix; required for the maintenance of Ssc1p solubility and assists in the functional interaction of Ssc1p with substrate proteins
5455YNL311CSKP2F-box protein of unknown functionF-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
5456YNL312WRFA2Subunit of heterotrimeric Replication Protein A (RPA)Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s
5457YNL313CEMW1Essential conserved protein with a role in cell wall integrityEssential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
5458YNL314WDAL82Positive regulator of allophanate inducible genesPositive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
5459YNL315CATP11Molecular chaperoneMolecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo
5460YNL316CPHA2Prephenate dehydratasePrephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
5461YNL317WPFS2Integral subunit of the pre-mRNA CPF complexIntegral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
5462YNL318CHXT14Protein with similarity to hexose transporter family membersProtein with similarity to hexose transporter family members; expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5463YNL319WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HXT14
5464YNL320WYNL320WPutative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5465YNL321WVNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membraneCalcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate
5466YNL322CKRE1Cell wall glycoprotein involved in beta-glucan assemblyCell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
5467YNL323WLEM3Membrane protein of the plasma membrane and ERMembrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
5468YNL324WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5469YNL325CFIG4Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatasePhosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder
5470YNL326CPFA3Palmitoyltransferase for Vac8pPalmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
5471YNL327WEGT2Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanaseGlycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase; required for proper cell separation after cytokinesis; expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner
5472YNL328CMDJ2Constituent of the mitochondrial import motorConstituent of the mitochondrial import motor; associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain
5473YNL329CPEX6AAA-peroxinAAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol
5474YNL330CRPD3Histone deacetylase, component of both the Rpd3S and Rpd3L complexesHistone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p
5475YNL331CAAD14Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
5476YNL332WTHI12Protein involved in synthesis of the thiamine precursor HMPProtein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
5477YNL333WSNZ2Member of a stationary phase-induced gene familyMember of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
5478YNL334CSNO2Protein of unknown functionProtein of unknown function; nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
5479YNL335WDDI3Cyanamide hydratase that detoxifies cyanamideProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
5480YNL336WCOS1Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
5481YNL337WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5482YNL338WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is a telomeric X element core sequence on the left arm of chromosome XIV
5483YNL339CYRF1-6Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
5484YNL339W-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5485YNL339W-BProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5486YNR001CCIT1Citrate synthaseCitrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
5487YNR001W-ADubious open reading frame unlikely to encode a functional protein; identified by homology
5488YNR002CATO2Putative transmembrane protein involved in export of ammoniaPutative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication
5489YNR003CRPC34RNA polymerase III subunit C34RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
5490YNR003W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene RPC34/YNR003C
5491YNR004WSWM2Protein with a role in snRNA and snoRNA cap trimethylationProtein with a role in snRNA and snoRNA cap trimethylation; interacts with Tgs1p and shows similar phenotypes; required for trimethylation of the caps of spliceosomal snRNAs and the U3 snoRNA, and for efficient 3' end processing of U3 snoRNA; may act as a specificity factor for Tgs1p
5492YNR005CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5493YNR006WVPS27Endosomal protein that forms a complex with Hse1pEndosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
5494YNR007CATG3E2-like enzymeE2-like enzyme; involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
5495YNR008WLRO1Acyltransferase that catalyzes diacylglycerol esterificationAcyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase
5496YNR009WNRM1Transcriptional co-repressor of MBF-regulated gene expressionTranscriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase
5497YNR010WCSE2Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia
5498YNR011CPRP2RNA-dependent DExD/H-box ATPaseRNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16
5499YNR012WURK1Uridine/cytidine kinaseUridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
5500YNR013CPHO91Low-affinity vacuolar phosphate transporterLow-affinity vacuolar phosphate transporter; exports phosphate from the vacuolar lumen to the cytosol; regulates phosphate and polyphosphate metabolism; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
5501YNR014WYNR014WPutative protein of unknown functionPutative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
5502YNR015WSMM1Dihydrouridine synthaseDihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
5503YNR016CACC1Acetyl-CoA carboxylase, biotin containing enzymeAcetyl-CoA carboxylase, biotin containing enzyme; catalyzes carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; ACC1 has a paralog, HFA1, that arose from the whole genome duplication
5504YNR017WTIM23Essential component of the TIM23 complexEssential component of the TIM23 complex; involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel; TIM23 complex is short for the translocase of the inner mitochondrial membrane
5505YNR018WRCF2Cytochrome c oxidase subunitCytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
5506YNR019WARE2Acyl-CoA:sterol acyltransferaseAcyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
5507YNR020CATP23Putative metalloprotease of the mitochondrial inner membranePutative metalloprotease of the mitochondrial inner membrane; required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
5508YNR021WYNR021WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene
5509YNR022CMRPL50Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
5510YNR023WSNF1273 kDa subunit of the SWI/SNF chromatin remodeling complex73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication
5511YNR024WMPP6Nuclear exosome-associated RNA binding proteinNuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia
5512YNR025CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
5513YNR026CSEC12Guanine nucleotide exchange factor (GEF)Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication
5514YNR027WBUD17Putative pyridoxal kinasePutative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
5515YNR028WCPR8Peptidyl-prolyl cis-trans isomerase (cyclophilin)Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; CPR8 has a paralog, CPR4, that arose from the whole genome duplication
5516YNR029CYNR029CPutative protein of unknown functionPutative protein of unknown function; deletion confers reduced fitness in saline
5517YNR030WALG12Alpha-1,6-mannosyltransferase localized to the ERAlpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
5518YNR031CSSK2MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathwayMAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
5519YNR032C-AHUB1Ubiquitin-like protein modifierUbiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
5520YNR032WPPG1Putative serine/threonine protein phosphatasePutative serine/threonine protein phosphatase; putative phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
5521YNR033WABZ1Para-aminobenzoate (PABA) synthasePara-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
5522YNR034WSOL1Protein with a possible role in tRNA exportProtein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
5523YNR034W-AEGO4Protein of unknown functionPutative protein of unknown function; expression is regulated by Msn2p/Msn4p; YNR034W-A has a paralog, YCR075W-A, that arose from the whole genome duplication
5524YNR035CARC35Subunit of the ARP2/3 complexSubunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
5525YNR036CMRPS12Mitochondrial proteinMitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
5526YNR037CRSM19Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; has similarity to E. coli S19 ribosomal protein
5527YNR038WDBP6Essential protein involved in ribosome biogenesisEssential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
5528YNR039CZRG17Endoplasmic reticulum zinc transporterEndoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
5529YNR040WDPI29Putative protein of unknown functionPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5530YNR041CCOQ2Para hydroxybenzoate polyprenyl transferasePara hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, is able to complement a yeast coq2 null mutant
5531YNR042WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
5532YNR043WMVD1Mevalonate pyrophosphate decarboxylaseMevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
5533YNR044WAGA1Anchorage subunit of a-agglutinin of a-cellsAnchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication
5534YNR045WPET494Mitochondrial translational activator specific for the COX3 mRNAMitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
5535YNR046WTRM112Protein involved in methylation of tRNA, rRNA, and translation factorsProtein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress
5536YNR047WFPK1Ser/Thr protein kinaseSer/Thr protein kinase; regulates the putative phospholipid translocases Lem3p-Dnf1p/Dnf2p; phosphorylates and inhibits upstream inhibitory kinase, Ypk1p; localizes to the cytoplasm, early endosome/TGN compartments, and plasma membrane; FPK1 has a paralog, KIN82, that arose from the whole genome duplication
5537YNR048WYNR048WPotential noncatalytic subunit for phospholipid translocase Dnf3pPotential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication
5538YNR049CMSO1Lipid-interacting protein in SNARE complex assembly machineryProbable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress
5539YNR050CLYS9Saccharopine dehydrogenase (NADP+, L-glutamate-forming)Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
5540YNR051CBRE5Ubiquitin protease cofactorUbiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
5541YNR052CPOP2RNase of the DEDD superfamilyRNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
5542YNR053CNOG2Putative GTPasePutative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
5543YNR054CESF2Essential nucleolar protein involved in pre-18S rRNA processingEssential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
5544YNR055CHOL1Putative transporter in the major facilitator superfamilyPutative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake
5545YNR056CBIO5Putative transmembrane protein involved in the biotin biosynthesisPutative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
5546YNR057CBIO4Dethiobiotin synthetaseDethiobiotin synthetase; catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria; expression appears to be repressed at low iron levels
5547YNR058WBIO37,8-diamino-pelargonic acid aminotransferase (DAPA)7,8-diamino-pelargonic acid aminotransferase (DAPA); catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria
5548YNR059WMNT4Putative alpha-1,3-mannosyltransferasePutative alpha-1,3-mannosyltransferase; not required for protein O-glycosylation
5549YNR060WFRE4Ferric reductaseFerric reductase; reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
5550YNR061CYNR061CProtein of unknown functionProtein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
5551YNR062CYNR062CPutative membrane protein of unknown functionPutative membrane protein of unknown function
5552YNR063WYNR063WPutative zinc-cluster protein of unknown functionPutative zinc-cluster protein of unknown function
5553YNR064CYNR064CEpoxide hydrolaseEpoxide hydrolase; member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
5554YNR065CYNR065CProtein of unknown functionProtein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
5555YNR066CYNR066CPutative membrane-localized protein of unknown functionPutative membrane-localized protein of unknown function
5556YNR067CDSE4Daughter cell-specific secreted protein with similarity to glucanasesDaughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother
5557YNR068CYNR068CPutative protein of unknown functionPutative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
5558YNR069CBSC5Protein of unknown functionProtein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
5559YNR070WPDR18Putative transporter of the ATP-binding cassette (ABC) familyPutative transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5560YNR071CYNR071CPutative aldose 1-epimerasePutative aldose 1-epimerase
5561YNR072WHXT17Putative transmembrane polyol transporterHexose transporter; up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose; HXT17 has a paralog, HXT13, that arose from a segmental duplication
5562YNR073CMAN2Mannitol dehydrogenasePutative mannitol dehydrogenase; YNR073C has a paralog, DSF1, that arose from a segmental duplication
5563YNR074CAIF1Mitochondrial cell death effectorMitochondrial cell death effector; translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase
5564YNR075C-AYNR075C-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5565YNR075WCOS10Endosomal protein involved in turnover of plasma membrane proteinsProtein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
5566YNR076WPAU6Member of the seripauperin multigene familyMember of the seripauperin multigene family; encoded mainly in subtelomeric regions; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme; identical to Pau18p
5567YNR077CYNR077CProtein of unknown function, abundance changes with carbon sourceProtein of unknown function, abundance changes with carbon source
5568YOL001WPHO80CyclinCyclin; interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
5569YOL002CIZH2Plasma membrane receptor for plant antifungal osmotinPlasma membrane receptor for plant antifungal protein, osmotin; involved in zinc ion homeostasis, apoptosis; negatively regulates ZRT1 and other functionally divergent genes through CCCTC promoter motif (IzRE); modulates FET3 activity in iron-independent manner; affects gene expression by influencing balance of competition between Msn2p/Msn4p and Nrg1p/Nrg2p for binding to the IzRE; transcription regulated by Zap1p, zinc, fatty acid levels; similar to mammalian adiponectins
5570YOL003CPFA4Palmitoyltransferase with autoacylation activityPalmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
5571YOL004WSIN3Component of both the Rpd3S and Rpd3L histone deacetylase complexesComponent of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
5572YOL005CRPB11RNA polymerase II subunit B12.5RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
5573YOL006CTOP1Topoisomerase ITopoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks
5574YOL007CCSI2Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
5575YOL008WCOQ10Coenzyme Q (ubiquinone) binding proteinCoenzyme Q (ubiquinone) binding protein; functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
5576YOL009CMDM12Mitochondrial outer membrane protein, ERMES complex subunitMitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
5577YOL010WRCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesisEndonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
5578YOL011WPLB3Phospholipase B (lysophospholipase) involved in lipid metabolismPhospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication
5579YOL012CHTZ1Histone variant H2AZHistone variant H2AZ; exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin; Htz1p-containing nucleosomes facilitate RNA Pol II passage by affecting correct assembly and modification status of RNA Pol II elongation complexes and by favoring efficient nucleosome remodeling
5580YOL013CHRD1Ubiquitin-protein ligase involved in ER-associated degradation (ERAD)Ubiquitin-protein ligase; functions in ER retention of misfolded proteins; required for ER-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
5581YOL013W-AYOL013W-APutative protein of unknown functionPutative protein of unknown function; identified by SAGE
5582YOL013W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element
5583YOL014WYOL014WPutative protein of unknown functionPutative protein of unknown function
5584YOL015WIRC10Protein of unknown functionPutative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
5585YOL016CCMK2Calmodulin-dependent protein kinaseCalmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
5586YOL017WESC8Protein involved in telomeric and mating-type locus silencingProtein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
5587YOL018CTLG2Syntaxin-like t-SNARESyntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
5588YOL019WYOL019WProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
5589YOL019W-AYOL019W-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5590YOL020WTAT2High affinity tryptophan and tyrosine permeaseHigh affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
5591YOL021CDIS3Exosome core complex catalytic subunitExosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; has similarity to E. coli RNase R and to human DIS3; mutations in Dis3p corresponding to human mutations implicated in multiple myeloma cause phenotypes suggesting impaired exosome function; protein abundance increases in response to DNA replication stress
5592YOL022CTSR4Cytoplasmic protein required for correct processing of 20S pre-rRNACytoplasmic protein required for correct processing of 20S pre-rRNA; protein required for processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
5593YOL023WIFM1Mitochondrial translation initiation factor 2Mitochondrial translation initiation factor 2
5594YOL024WYOL024WPutative protein of unknown functionPutative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication
5595YOL025WLAG2Protein that negatively regulates the SCF E3-ubiquitin ligaseProtein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1
5596YOL026CMIM1Mitochondrial protein required for outer membrane protein importMitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
5597YOL027CMDM38Mitochondrial proteinMitochondrial protein; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome
5598YOL028CYAP7Putative basic leucine zipper (bZIP) transcription factorPutative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
5599YOL029CYOL029CPutative protein of unknown functionPutative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
5600YOL030WGAS51,3-beta-glucanosyltransferase1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall
5601YOL031CSIL1Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2pNucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein
5602YOL032WOPI10Protein with a possible role in phospholipid biosynthesisProtein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
5603YOL033WMSE1Mitochondrial glutamyl-tRNA synthetaseMitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated
5604YOL034WSMC5Subunit of the SMC5-SMC6 complexComponent of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
5605YOL035CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5606YOL036WYOL036WProtein of unknown functionProtein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
5607YOL037CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W
5608YOL038C-AYOL038C-APutative protein of unknown functionPutative protein of unknown function; identified by SAGE analysis
5609YOL038WPRE6Alpha 4 subunit of the 20S proteasomeAlpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
5610YOL039WRPP2ARibosomal protein P2 alphaRibosomal protein P2 alpha; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
5611YOL040CRPS15Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
5612YOL041CNOP12Nucleolar protein involved in pre-25S rRNA processingNucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
5613YOL042WNGL1Putative endonucleasePutative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5614YOL043CNTG2DNA N-glycosylase and apurinic/apyrimidinic (AP) lyaseDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
5615YOL044WPEX15Tail-anchored type II integral peroxisomal membrane proteinTail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly
5616YOL045WPSK2PAS-domain containing serine/threonine protein kinasePAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
5617YOL046CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene PSK2/YOL045W
5618YOL047CLDS2Protein Involved in spore wall assemblyProtein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds1p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
5619YOL048CRRT8Protein involved in spore wall assemblyProtein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
5620YOL049WGSH2Glutathione synthetaseGlutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
5621YOL050CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
5622YOL051WGAL11Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
5623YOL052CSPE2S-adenosylmethionine decarboxylaseS-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
5624YOL052C-ADDR2Multi-stress response proteinMulti-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
5625YOL053WAIM39Protein of unknown functionPutative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
5626YOL054WPSH1E3 ubiquitin ligase targeting centromere-binding protein Cse4pE3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates poyubiquitination and degradation of Cse4p; prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p
5627YOL055CTHI20Trifunctional enzyme of thiamine biosynthesis, degradation and salvageTrifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
5628YOL056WGPM3Nonfunctional homolog of Gpm1p phosphoglycerate mutaseHomolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
5629YOL057WYOL057WDipeptidyl-peptidase IIIDipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
5630YOL058WARG1Arginosuccinate synthetaseArginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
5631YOL059WGPD2NAD-dependent glycerol 3-phosphate dehydrogenaseNAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication
5632YOL060CMAM3Protein required for normal mitochondrial morphologyProtein required for normal mitochondrial morphology; has similarity to hemolysins
5633YOL061WPRS55-phospho-ribosyl-1(alpha)-pyrophosphate synthetase5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
5634YOL062CAPM4Cargo-binding mu subunit of AP-2Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals
5635YOL063CCRT10Protein involved in transcriptional regulation of RNR2 and RNR3Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat
5636YOL064CMET22Bisphosphate-3'-nucleotidaseBisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
5637YOL065CINP54Phosphatidylinositol 4,5-bisphosphate 5-phosphatasePhosphatidylinositol 4,5-bisphosphate 5-phosphatase; role in secretion; localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
5638YOL066CRIB2Bifunctional DRAP deaminase tRNA:pseudouridine synthaseBifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
5639YOL067CRTG1Transcription factor (bHLH) involved in interorganelle communicationTranscription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization
5640YOL068CHST1NAD(+)-dependent histone deacetylaseNAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication
5641YOL069WNUF2Component of the kinetochore-associated Ndc80 complexComponent of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
5642YOL070CNBA1Protein of unknown functionProtein of unknown function; localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate
5643YOL071WSDH5Protein required for flavinylation of Sdh1pProtein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
5644YOL072WTHP1Nuclear pore-associated proteinNuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
5645YOL073CDSC2Multi-transmembrane subunit of the DSC ubiquitin ligase complexMulti-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2
5646YOL075CYOL075CPutative ABC transporterPutative ABC transporter
5647YOL076WMDM20Non-catalytic subunit of the NatB N-terminal acetyltransferaseNon-catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
5648YOL077CBRX1Nucleolar proteinNucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
5649YOL077W-AATP19Subunit k of the mitochondrial F1F0 ATP synthaseSubunit k of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
5650YOL078WAVO1Component of a membrane-bound complex containing the Tor2p kinaseComponent of a membrane-bound complex containing the Tor2p kinase; contains Tor2p kinase and other proteins; may have a role in regulation of cell growth
5651YOL079WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5652YOL080CREX4Putative RNA exonucleasePutative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly
5653YOL081WIRA2GTPase-activating proteinGTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
5654YOL082WATG19Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathwayReceptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
5655YOL083C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry
5656YOL083WATG34Receptor protein involved in selective autophagy during starvationReceptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
5657YOL084WPHM7Protein of unknown functionProtein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
5658YOL085CYOL085CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A
5659YOL085W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YOL085C
5660YOL086CADH1Alcohol dehydrogenaseAlcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication
5661YOL086W-AMHF1Component of the heterotetrameric MHF histone-fold complexComponent of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1
5662YOL087CDUF1Ubiquitin-binding protein of unknown functionUbiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
5663YOL088CMPD2Member of the protein disulfide isomerase (PDI) familyMember of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
5664YOL089CHAL9Putative transcription factor containing a zinc fingerPutative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
5665YOL090WMSH2Protein that binds to DNA mismatchesProtein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
5666YOL091WSPO21Component of the meiotic outer plaque of the spindle pole bodyComponent of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation; SPO21 has a paralog, YSW1, that arose from the whole genome duplication
5667YOL092WYPQ1Putative vacuolar membrane transporter for cationic amino acidsPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication
5668YOL093WTRM10tRNA methyltransferasetRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator
5669YOL094CRFC4Subunit of heteropentameric Replication factor C (RF-C)Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
5670YOL095CHMI1Mitochondrial inner membrane localized ATP-dependent DNA helicaseMitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
5671YOL096CCOQ3O-methyltransferaseO-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
5672YOL097CWRS1Cytoplasmic tryptophanyl-tRNA synthetaseCytoplasmic tryptophanyl-tRNA synthetase; aminoacylates tryptophanyl-tRNA
5673YOL097W-AYOL097W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
5674YOL098CSDD3Putative metalloproteasePutative metalloprotease
5675YOL099CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
5676YOL100WPKH2Serine/threonine protein kinaseSerine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
5677YOL101CIZH4Membrane protein involved in zinc ion homeostasisMembrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication
5678YOL102CTPT1tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicingtRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
5679YOL103WITR2Myo-inositol transporterMyo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication
5680YOL103W-AYOL103W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
5681YOL103W-BYOL103W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
5682YOL104CNDJ1Protein that regulates meiotic SPB cohesion and telomere clusteringMeiosis-specific telomere protein; required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
5683YOL105CWSC3Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathwaySensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication
5684YOL106WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5685YOL107WYOL107WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein
5686YOL108CINO4Transcription factor involved in phospholipid synthesisTranscription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
5687YOL109WZEO1Peripheral membrane protein of the plasma membranePeripheral membrane protein of the plasma membrane; interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
5688YOL110WSHR5Palmitoyltransferase subunitPalmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2
5689YOL111CMDY2Protein involved in inserting tail-anchored proteins into ER membranesProtein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
5690YOL112WMSB4GTPase-activating protein of the Ras superfamilyGTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication
5691YOL113WSKM1Member of the PAK family of serine/threonine protein kinasesMember of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication
5692YOL114CPTH4Protein similar to the human peptidyl-tRNA hydrolase gene ICT1Putative protein of unknown function with similarity to human ICT1; has prokaryotic factors that may function in translation termination; YOL114C is not an essential gene
5693YOL115WPAP2Non-canonical poly(A) polymeraseNon-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia
5694YOL116WMSN1Transcriptional activatorTranscriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
5695YOL117WRRI2Subunit of the COP9 signalosome (CSN) complexSubunit of the COP9 signalosome (CSN) complex; this complex cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
5696YOL118CYOL118CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5697YOL119CMCH4Protein with similarity to mammalian monocarboxylate permeasesProtein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
5698YOL120CRPL18ARibosomal 60S subunit protein L18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
5699YOL121CRPS19AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
5700YOL122CSMF1Divalent metal ion transporterDivalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
5701YOL123WHRP1Subunit of cleavage factor ISubunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability
5702YOL124CTRM11Catalytic subunit of adoMet-dependent tRNA methyltransferase complexCatalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p
5703YOL125WTRM132'-O-methyltransferase2'-O-methyltransferase; responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
5704YOL126CMDH2Cytoplasmic malate dehydrogenaseCytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
5705YOL127WRPL25Ribosomal 60S subunit protein L25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
5706YOL128CYGK3Protein kinase related to mammalian GSK-3 glycogen synthase kinasesProtein kinase related to mammalian GSK-3 glycogen synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation; YGK3 has a paralog, MCK1, that arose from the whole genome duplication
5707YOL129WVPS68Vacuolar membrane protein of unknown functionVacuolar membrane protein of unknown function; involved in vacuolar protein sorting; also detected in the mitochondria
5708YOL130WALR1Plasma membrane Mg(2+) transporterPlasma membrane Mg(2+) transporter; expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
5709YOL131WYOL131WPutative protein of unknown functionPutative protein of unknown function; YOL131W has a paralog, STB1, that arose from the whole genome duplication
5710YOL132WGAS41,3-beta-glucanosyltransferase1,3-beta-glucanosyltransferase; involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall
5711YOL133WHRT1RING-H2 domain core subunit of multiple ubiquitin ligase complexesRING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair
5712YOL134CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
5713YOL135CMED7Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
5714YOL136CPFK276-phosphofructo-2-kinase6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
5715YOL137WBSC6Protein of unknown function with 8 putative transmembrane segmentsProtein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
5716YOL138CRTC1Subunit of SEACAT, a subcomplex of the SEA complexSubunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
5717YOL139CCDC33mRNA cap binding protein and translation initiation factor eIF4EmRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
5718YOL140WARG8Acetylornithine aminotransferaseAcetylornithine aminotransferase; catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
5719YOL141WPPM2AdoMet-dependent tRNA methyltransferaseAdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
5720YOL142WRRP40Exosome non-catalytic core componentExosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain both S1 and KH RNA binding domains; has similarity to human hRrp40p (EXOSC3)
5721YOL143CRIB4Lumazine synthase (DMRL synthase)Lumazine synthase (DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin; DMRL synthase stands for 6,7-dimethyl-8-ribityllumazine synthase
5722YOL144WNOP8Nucleolar protein required for 60S ribosomal subunit biogenesisNucleolar protein required for 60S ribosomal subunit biogenesis
5723YOL145CCTR9Component of the Paf1p complex involved in transcription elongationComponent of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
5724YOL146WPSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
5725YOL147CPEX11Peroxisomal protein required for medium-chain fatty acid oxidationPeroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
5726YOL148CSPT20Subunit of the SAGA transcriptional regulatory complexSubunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
5727YOL149WDCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complexSubunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
5728YOL150CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5729YOL151WGRE23-methylbutanal reductase and NADPH-dependent methylglyoxal reductase3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase
5730YOL152WFRE7Putative ferric reductase with similarity to Fre2pPutative ferric reductase with similarity to Fre2p; expression induced by low copper levels
5731YOL154WZPS1Putative GPI-anchored proteinPutative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH
5732YOL155CHPF1Haze-protective mannoproteinHaze-protective mannoprotein; reduces the particle size of aggregated proteins in white wines
5733YOL155W-AYOL155W-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
5734YOL156WHXT11Hexose transporterPutative hexose transporter that is nearly identical to Hxt9p; has similarity to major facilitator superfamily (MFS) transporters and is involved in pleiotropic drug resistance
5735YOL157CIMA2Isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase)Isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; not required for isomaltose utilization, but Ima2p overexpression allows the ima1 null mutant to grow on isomaltose
5736YOL158CENB1Endosomal ferric enterobactin transporterEndosomal ferric enterobactin transporter; expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
5737YOL159CCSS3Protein of unknown function, secreted when constitutively expressedSoluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA
5738YOL159C-AYOL159C-AProtein of unknown functionProtein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
5739YOL160WYOL160WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5740YOL161CPAU20Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression induced by low temperature and also by anaerobic conditions; induced during alcoholic fermentation
5741YOL162WYOL162WPutative protein of unknown functionPutative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
5742YOL163WYOL163WPutative protein of unknown functionPutative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
5743YOL164WBDS1Bacterially-derived sulfataseBacterially-derived sulfatase; required for use of alkyl- and aryl-sulfates as sulfur sources
5744YOL164W-AYOL164W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
5745YOL165CAAD15Putative aryl-alcohol dehydrogenasePutative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD15 has a paralog, AAD3, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
5746YOL166CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5747YOL166W-AYOL166W-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5748YOR001WRRP6Nuclear exosome exonuclease componentNuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based
5749YOR002WALG6Alpha 1,3 glucosyltransferaseAlpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
5750YOR003WYSP3Putative precursor of the subtilisin-like protease IIIPutative precursor to the subtilisin-like protease III; YSP3 has a paralog, PRB1, that arose from the whole genome duplication
5751YOR004WUTP23Component of the small subunit processomeComponent of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
5752YOR005CDNL4DNA ligase required for nonhomologous end-joining (NHEJ)DNA ligase required for nonhomologous end-joining (NHEJ); forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth
5753YOR006CTSR3Protein required for 20S pre-rRNA processingProtein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
5754YOR007CSGT2Glutamine-rich cytoplasmic cochaperoneGlutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
5755YOR008CSLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathwaySensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
5756YOR008C-AYOR008C-APutative protein of unknown functionPutative protein of unknown function; includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
5757YOR008W-BDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5758YOR009WTIR4Cell wall mannoproteinCell wall mannoprotein; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
5759YOR010CTIR2Putative cell wall mannoproteinPutative cell wall mannoprotein; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis; TIR2 has a paralog, TIR3, that arose from the whole genome duplication
5760YOR011WAUS1Plasma membrane sterol transporter of the ATP-binding cassette familyPlasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication
5761YOR011W-AYOR011W-APutative protein of unknown functionPutative protein of unknown function
5762YOR012WYOR012WPutative protein of unknown functionPutative protein of unknown function
5763YOR013WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci
5764YOR014WRTS1B-type regulatory subunit of protein phosphatase 2A (PP2A)B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
5765YOR015WYOR015WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5766YOR016CERP4Member of the p24 family involved in ER to Golgi transportMember of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication
5767YOR017WPET127Protein with a role in 5'-end processing of mitochondrial RNAsProtein with a role in 5'-end processing of mitochondrial RNAs; located in the mitochondrial membrane
5768YOR018WROD1Alpha-arrestin involved in ubiquitin-dependent endocytosisAlpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication
5769YOR019WYOR019WProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; YOR019W has a paralog, JIP4, that arose from the whole genome duplication
5770YOR020CHSP10Mitochondrial matrix co-chaperoninMitochondrial matrix co-chaperonin; inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
5771YOR020W-AMCO10Putative protein of unknown functionPutative protein of unknown function; conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5772YOR021CSFM1SPOUT methyltransferaseSPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
5773YOR022CDDL1DDHD domain-containing phospholipase A1Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
5774YOR023CAHC1Subunit of the Ada histone acetyltransferase complexSubunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex
5775YOR024WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5776YOR025WHST3Member of the Sir2 family of NAD(+)-dependent protein deacetylasesMember of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
5777YOR026WBUB3Kinetochore checkpoint WD40 repeat proteinKinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p
5778YOR027WSTI1Hsp90 cochaperoneHsp90 cochaperone; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
5779YOR028CCIN5Basic leucine zipper (bZIP) transcription factor of the yAP-1 familyBasic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
5780YOR029WYOR029WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5781YOR030WDFG16Probable multiple transmembrane proteinProbable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
5782YOR031WCRS5Copper-binding metallothioneinCopper-binding metallothionein; required for wild-type copper resistance
5783YOR032CHMS1bHLH protein with similarity to myc-family transcription factorsbHLH protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
5784YOR032W-AYOR032W-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5785YOR033CEXO15'-3' exonuclease and flap-endonuclease5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
5786YOR034CAKR2Ankyrin repeat-containing proteinAnkyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
5787YOR034C-AYOR034C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
5788YOR035CSHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domainProtein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
5789YOR036WPEP12Target membrane receptor (t-SNARE)Target membrane receptor (t-SNARE); for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
5790YOR037WCYC2Mitochondrial peripheral inner membrane proteinMitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
5791YOR038CHIR2Subunit of HIR nucleosome assembly complexSubunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia
5792YOR039WCKB2Beta' regulatory subunit of casein kinase 2 (CK2)Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
5793YOR040WGLO4Mitochondrial glyoxalase IIMitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication
5794YOR041CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance
5795YOR042WCUE5Ubiquitin-binding proteinUbiquitin-binding protein; functions as a ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; contains a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; GFP-fusion protein localizes to the cytoplasm in a punctate pattern; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
5796YOR043WWHI2Protein required for full activation of the general stress responseProtein required for full activation of the general stress response; required with binding partner Psr1p, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression
5797YOR044WIRC23Protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication
5798YOR045WTOM6Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
5799YOR046CDBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box familyCytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
5800YOR047CSTD1Protein involved in control of glucose-regulated gene expressionProtein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]; STD1 has a paralog, MTH1, that arose from the whole genome duplication
5801YOR048CRAT1Nuclear 5' to 3' single-stranded RNA exonucleaseNuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage
5802YOR049CRSB1Putative sphingoid long-chain base (LCB) efflux transporterSuppressor of sphingoid LCB sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport long chain bases (LCBs) from the cytoplasmic side toward the extracytoplasmic side of the membrane
5803YOR050CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant is viable
5804YOR051CETT1Nuclear protein that inhibits replication of Brome mosaic virusNuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
5805YOR052CTMC1AN1-type zinc finger protein, effector of proteotoxic stress responseAN1-type zinc finger protein of unknown function; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element, a transcriptional profile similar to CUZ1 and RPN2, and decreased expression in an RPN4 mutant; induced by nitrogen limitation and weak acid; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
5806YOR053WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
5807YOR054CVHS3Negative regulatory subunit of protein phosphatase 1 Ppz1pNegative regulatory subunit of protein phosphatase 1 Ppz1p; involved in coenzyme A biosynthesis; subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p)
5808YOR055WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5809YOR056CNOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesisProtein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
5810YOR057WSGT1Cochaperone proteinCochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
5811YOR058CASE1Mitotic spindle midzone-localized microtubule bundling proteinMitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
5812YOR059CLPL1PhospholipasePhospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
5813YOR060CSLD7Protein with a role in chromosomal DNA replicationProtein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
5814YOR061WCKA2Alpha' catalytic subunit of casein kinase 2 (CK2)Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
5815YOR062CYOR062CProtein of unknown functionProtein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication
5816YOR063WRPL3Ribosomal 60S subunit protein L3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; plays an important role in function of eIF5B in stimulating 3' end processing of 18S rRNA in context of 80S ribosomes that have not yet engaged in translation; involved in replication and maintenance of killer double stranded RNA virus
5817YOR064CYNG1Subunit of the NuA3 histone acetyltransferase complexSubunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
5818YOR065WCYT1Cytochrome c1Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
5819YOR066WMSA1Activator of G1-specific transcription factors MBF and SBFActivator of G1-specific transcription factors MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that arose from the whole genome duplication
5820YOR067CALG8Glucosyl transferaseGlucosyl transferase; involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
5821YOR068CVAM10Protein involved in vacuole morphogenesisProtein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
5822YOR069WVPS5Nexin-1 homologNexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication
5823YOR070CGYP1Cis-golgi GTPase-activating protein (GAP) for yeast RabsCis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
5824YOR071CNRT1High-affinity nicotinamide riboside transporterHigh-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; major transporter for 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine) uptake; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity
5825YOR072WYOR072WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
5826YOR072W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR072W; originally identified by fungal homology and RT-PCR
5827YOR072W-BYOR072W-BPutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
5828YOR073WSGO1Component of the spindle checkpointComponent of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
5829YOR073W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CDC21/YOR074C; identified by RT-PCR
5830YOR074CCDC21Thymidylate synthaseThymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
5831YOR075WUFE1t-SNARE protein required for retrograde vesicular traffict-SNARE protein required for retrograde vesicular traffic; also required for homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
5832YOR076CSKI7GTP-binding protein that couples the Ski complex and exosomeCoupling protein for the Ski complex and cytoplasmic exosome; involved in 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
5833YOR077WRTS2Basic zinc-finger proteinBasic zinc-finger protein; similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
5834YOR078WBUD21Component of small ribosomal subunit (SSU) processosomeComponent of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern
5835YOR079CATX2Golgi membrane protein involved in manganese homeostasisGolgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation
5836YOR080WDIA2Origin-binding F-box proteinOrigin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitylation of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth
5837YOR081CTGL5Bifunctional triacylglycerol lipase and LPA acyltransferaseBifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication
5838YOR082CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR083W
5839YOR083WWHI5Repressor of G1 transcriptionRepressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1; WHI5 has a paralog, SRL3, that arose from the whole genome duplication
5840YOR084WLPX1Peroxisomal matrix-localized lipasePeroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible
5841YOR085WOST3Gamma subunit of the oligosaccharyltransferase complex of the ER lumenGamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
5842YOR086CTCB1Lipid-binding ER protein involved in ER-plasma membrane tetheringLipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in PM; contains 3 calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact; TCB1 has a paralog, TCB2, that arose from the whole genome duplication
5843YOR087WYVC1Vacuolar cation channelVacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
5844YOR089CVPS21Endosomal Rab family GTPaseEndosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
5845YOR090CPTC5Mitochondrial type 2C protein phosphatase (PP2C)Mitochondrial type 2C protein phosphatase (PP2C); involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
5846YOR091WTMA46Protein of unknown function that associates with translating ribosomesProtein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
5847YOR092WECM3Non-essential protein of unknown functionNon-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
5848YOR093CCMR2Putative protein of unknown functionPutative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
5849YOR094WARF3Glucose-repressible ADP-ribosylation factorGlucose-repressible ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulating cell polarity and invasive growth; also has mRNA binding activity
5850YOR095CRKI1Ribose-5-phosphate ketol-isomeraseRibose-5-phosphate ketol-isomerase; catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
5851YOR096WRPS7AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication
5852YOR097CYOR097CPutative protein of unknown functionPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
5853YOR098CNUP1FG-nucleoporin component of central core of the nuclear pore complexFG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
5854YOR099WKTR1Alpha-1,2-mannosyltransferaseAlpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress
5855YOR100CCRC1Mitochondrial inner membrane carnitine transporterMitochondrial inner membrane carnitine transporter; required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation
5856YOR101WRAS1GTPase involved in G-protein signaling in adenylate cyclase activationGTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
5857YOR102WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
5858YOR103COST2Epsilon subunit of the oligosaccharyltransferase complexEpsilon subunit of the oligosaccharyltransferase complex; located in the ER lumen; catalyzes asparagine-linked glycosylation of newly synthesized proteins
5859YOR104WPIN2Exomer-dependent cargo proteinExomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated
5860YOR105WYOR105WProtein of unknown functionProtein of unknown function; expressed at both mRNA and protein levels
5861YOR106WVAM3Syntaxin-like vacuolar t-SNARESyntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
5862YOR107WRGS2Negative regulator of glucose-induced cAMP signalingNegative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
5863YOR108C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5864YOR108WLEU9Alpha-isopropylmalate synthase II (2-isopropylmalate synthase)Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
5865YOR109WINP53Polyphosphatidylinositol phosphatasePolyphosphatidylinositol phosphatase; dephosphorylates multiple phosphatidylinositol phosphates; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; INP53 has a paralog, INP52, that arose from the whole genome duplication
5866YOR110WTFC7RNA pol III transcription initiation factor complex (TFIIIC) subunitRNA pol III transcription initiation factor complex (TFIIIC) subunit; part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; TFC7 has a paralog, YNL108C, that arose from the whole genome duplication
5867YOR111WYOR111WPutative protein of unknown functionPutative protein of unknown function
5868YOR112WCEX1Component of nuclear aminoacylation-dependent tRNA export pathwayComponent of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex
5869YOR113WAZF1Zinc-finger transcription factorZinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia
5870YOR114WDPI34Putative protein of unknown functionPutative protein of unknown function; null mutant is viable
5871YOR115CTRS33Core component of TRAPP complexes I, II and IVCore component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII)
5872YOR116CRPO31RNA polymerase III largest subunit C160RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
5873YOR117WRPT5ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
5874YOR118WRTC5Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
5875YOR119CRIO1Serine kinase involved in cell cycle regulation and rDNA integritySerine kinase involved in cell cycling and pre-rRNA processing; associated with late pre-40S particles via its conserved C-terminal domain and participates in late 40S biogenesis; association with pre-40S particles regulated by its catalytic ATPase site and likely occurs after the release of Rio2p from these particles; involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA; essential gene
5876YOR120WGCY1Glycerol dehydrogenaseGlycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
5877YOR121CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W
5878YOR122CPFY1ProfilinProfilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
5879YOR123CLEO1Component of the Paf1 complexComponent of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
5880YOR124CUBP2Ubiquitin-specific proteaseUbiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
5881YOR125CCAT5Protein required for ubiquinone (Coenzyme Q) biosynthesisProtein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
5882YOR126CIAH1Isoamyl acetate-hydrolyzing esteraseIsoamyl acetate-hydrolyzing esterase; required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
5883YOR127WRGA1GTPase-activating protein for polarity-establishment protein Cdc42pGTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication
5884YOR128CADE2Phosphoribosylaminoimidazole carboxylasePhosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
5885YOR129CAFI1Arf3p polarization-specific docking factorArf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
5886YOR130CORT1Ornithine transporter of the mitochondrial inner membraneOrnithine transporter of the mitochondrial inner membrane; exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
5887YOR131CYOR131CPutative haloacid dehalogenase-like hydrolasePutative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
5888YOR132WVPS17Subunit of the membrane-associated retromer complexSubunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
5889YOR133WEFT1Elongation factor 2 (EF-2), also encoded by EFT2Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
5890YOR134WBAG7Rho GTPase activating protein (RhoGAP)Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication
5891YOR135CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
5892YOR136WIDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenaseSubunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
5893YOR137CSIA1Protein of unassigned functionProtein of unassigned function; involved in activation of the Pma1p plasma membrane H+-ATPase by glucose; contains peptide signal for membrane localization
5894YOR138CRUP1Protein that regulates ubiquitination of Rsp5pProtein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
5895YOR139CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
5896YOR140WSFL1Transcriptional repressor and activatorTranscriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C)
5897YOR141CARP8Nuclear actin-related protein involved in chromatin remodelingNuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; has mRNA binding activity
5898YOR142WLSC1Alpha subunit of succinyl-CoA ligaseAlpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
5899YOR142W-AYOR142W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
5900YOR142W-BYOR142W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
5901YOR143CTHI80Thiamine pyrophosphokinaseThiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
5902YOR144CELG1Subunit of an alternative replication factor C complexSubunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
5903YOR145CPNO1Essential nucleolar protein required for pre-18S rRNA processingEssential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain
5904YOR146WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
5905YOR147WMDM32Mitochondrial inner membrane protein with similarity to Mdm31pMitochondrial inner membrane protein with similarity to Mdm31p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; variation between SK1 and S288C at residues 182 and 262 impacts invasive growth and mitochondrial network structure
5906YOR148CSPP2Essential protein that promotes the first step of splicingEssential protein that promotes the first step of splicing; required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
5907YOR149CSMP3Alpha 1,2-mannosyltransferaseAlpha 1,2-mannosyltransferase; involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
5908YOR150WMRPL23Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
5909YOR151CRPB2RNA polymerase II second largest subunit B150RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
5910YOR152CATG40Autophagy receptor with a role in endoplasmic reticulum degradationPutative protein of unknown function; YOR152C is not an essential gene
5911YOR153WPDR5Plasma membrane ATP-binding cassette (ABC) transporterPlasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
5912YOR154WSLP1Glycosylated integral ER membrane protein of unknown functionGlycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
5913YOR155CISN1Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidaseInosine 5'-monophosphate (IMP)-specific 5'-nucleotidase; catalyzes the breakdown of IMP to inosine; responsible for production of nicotinamide riboside and nicotinic acid riboside; expression positively regulated by nicotinic acid and glucose availability; does not show similarity to known 5'-nucleotidases from other organisms
5914YOR156CNFI1SUMO E3 ligaseSUMO E3 ligase; catalyzes sumoylation of Yku70p/Yku80p and Sir4p promoting chromatin anchoring; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length; NFI1 has a paralog, SIZ1, that arose from the whole genome duplication
5915YOR157CPUP1Beta 2 subunit of the 20S proteasomeBeta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
5916YOR158WPET123Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
5917YOR159CSME1Core Sm protein Sm ECore Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
5918YOR160WMTR10Nuclear import receptorNuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
5919YOR161CPNS1Protein of unknown functionProtein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
5920YOR161C-CYOR161C-CProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5921YOR161W-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5922YOR161W-BProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5923YOR162CYRR1Zn2-Cys6 zinc-finger transcription factorZn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
5924YOR163WDDP1Polyphosphate phosphatasePolyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
5925YOR164CGET4Protein involved in inserting tail-anchored proteins into ER membranesProtein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
5926YOR165WSEY1Dynamin-like GTPase that mediates homotypic ER fusionDynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
5927YOR166CSWT1RNA endoribonuclease involved in perinuclear mRNP quality controlRNA endoribonuclease involved in perinuclear mRNP quality control; involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
5928YOR167CRPS28AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in regulation of RPS28B, in which Rps28Ap binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28A has a paralog, RPS28B, that arose from the whole genome duplication
5929YOR168WGLN4Glutamine tRNA synthetaseGlutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
5930YOR169CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
5931YOR170WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4
5932YOR171CLCB4Sphingoid long-chain base kinaseSphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication
5933YOR172WYRM1Zinc finger transcription factor involved in multidrug resistanceZinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
5934YOR173WDCS2m(7)GpppX pyrophosphatase regulatorm(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication
5935YOR174WMED4Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
5936YOR175CALE1Broad-specificity lysophospholipid acyltransferaseBroad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
5937YOR176WHEM15FerrochelataseFerrochelatase; a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
5938YOR177CMPC54Component of the meiotic outer plaqueComponent of the meiotic outer plaque; a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
5939YOR178CGAC1Regulatory subunit for Glc7p type-1 protein phosphatase (PP1)Regulatory subunit for Glc7p type-1 protein phosphatase (PP1); tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; GAC1 has a paralog, PIG1, that arose from the whole genome duplication
5940YOR179CSYC1Subunit of the APT subcomplex of cleavage and polyadenylation factorSubunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
5941YOR180CDCI1Peroxisomal proteinPeroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed; DCI1 has a paralog, ECI1, that arose from the whole genome duplication
5942YOR181WLAS17Actin assembly factorActin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments and a polyproline domain which can nucleate actin filaments independent of Arp2/3; mutants are defective in actin cytoskeleton dependent processes such as: endocytosis, bud site selection and cytokinesis; localizes with the Arp2/3 complex to actin cortical patches; homolog of the Wiskott-Aldrich Syndrome protein (WASP), implicated in severe immunodeficiency
5943YOR182CRPS30BProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30B has a paralog, RPS30A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
5944YOR183WFYV12Protein of unknown functionProtein of unknown function; required for survival upon exposure to K1 killer toxin
5945YOR184WSER13-phosphoserine aminotransferase3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
5946YOR185CGSP2GTP binding protein (mammalian Ranp homolog)GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication
5947YOR186C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5948YOR186WYOR186WPutative protein of unknown functionPutative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication
5949YOR187WTUF1Mitochondrial translation elongation factor Tu (EF-Tu)Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans
5950YOR188WMSB1Protein of unknown functionProtein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
5951YOR189WIES4Component of the INO80 chromatiin remodeling complexComponent of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
5952YOR190WSPR1Sporulation-specific exo-1,3-beta-glucanaseSporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance; SPR1 has a paralog, EXG1, that arose from the whole genome duplication
5953YOR191WULS1Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL)Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress
5954YOR192CTHI72Transporter of thiamine or related compoundTransporter of thiamine or related compound; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); shares sequence similarity with Thi7p
5955YOR192C-AYOR192C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
5956YOR192C-BYOR192C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
5957YOR192C-CYOR192C-CPutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
5958YOR193WPEX27Peripheral peroxisomal membrane proteinPeripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication
5959YOR194CTOA1TFIIA large subunitTFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa2p, for ribosomal protein gene transcription in vivo; homologous to largest and second largest subunits of human and Drosophila TFIIA
5960YOR195WSLK19Kinetochore-associated proteinKinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
5961YOR196CLIP5Protein involved in biosynthesis of the coenzyme lipoic acidProtein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
5962YOR197WMCA1Ca2+-dependent cysteine proteaseCa2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization
5963YOR198CBFR1Component of mRNP complexes associated with polyribosomesComponent of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
5964YOR199WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5965YOR200WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
5966YOR201CMRM1Ribose methyltransferaseRibose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
5967YOR202WHIS3Imidazoleglycerol-phosphate dehydrataseImidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
5968YOR203WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
5969YOR204WDED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicaseATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase; required for translation initiation of all yeast mRNAs; binds to mRNA cap-associated factors, and binding stimulates Ded1p RNA-dependent ATPase activity; mutation in human homolog DBY is associated with male infertility; human homolog DDX3X complements ded1 null mutation; DED1 has a paralog, DBP1, that arose from the whole genome duplication
5970YOR205CGEP3Protein required for mitochondrial ribosome small subunit biogenesisProtein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
5971YOR206WNOC2Protein involved in ribosome biogenesisProtein involved in ribosome biogenesis; forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes; forms a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit
5972YOR207CRET1Second-largest subunit of RNA polymerase IIISecond-largest subunit of RNA polymerase III; RNA polymerase III is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
5973YOR208WPTP2Nuclear phosphotyrosine-specific phosphatase involved in osmosensingNuclear phosphotyrosine-specific phosphatase involved in osmosensing; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; with Msg5p co-regulates the calcium signaling pathway
5974YOR209CNPT1Nicotinate phosphoribosyltransferaseNicotinate phosphoribosyltransferase; acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
5975YOR210WRPB10RNA polymerase subunit ABC10-betaRNA polymerase subunit ABC10-beta; common to RNA polymerases I, II, and III
5976YOR211CMGM1Mitochondrial GTPase, present in complex with Ugo1p and Fzo1pMitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy
5977YOR212WSTE4G protein beta subunitG protein beta subunit; forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats
5978YOR213CSAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p)Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
5979YOR214CSPR2Putative spore wall proteinPutative spore wall protein; expression increases during sporulation; not an essential gene; YOR214C has a paralog, SPO19, that arose from the whole genome duplication
5980YOR215CAIM41Protein of unknown functionPutative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
5981YOR216CRUD3Golgi matrix proteinGolgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
5982YOR217WRFC1Subunit of heteropentameric Replication factor C (RF-C)Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
5983YOR218CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
5984YOR219CSTE13Dipeptidyl aminopeptidaseDipeptidyl aminopeptidase; Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor
5985YOR220WRCN2Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
5986YOR221CMCT1Predicted malonyl-CoA:ACP transferasePredicted malonyl-CoA:ACP transferase; putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
5987YOR222WODC2Mitochondrial inner membrane transporterMitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism; ODC2 has a paralog, ODC1, that arose from the whole genome duplication
5988YOR223WDSC3Subunit of the DSC ubiquitin ligase complexSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
5989YOR224CRPB8RNA polymerase subunit ABC14.5RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III
5990YOR225WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
5991YOR226CISU2Mitochondrial protein required for iron-sulfur protein synthesisProtein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved; ISU2 has a paralog, ISU1, that arose from the whole genome duplication
5992YOR227WHER1Protein of unknown functionProtein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
5993YOR228CMCP1Mitochondrial protein of unknown function involved in lipid homeostasMitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains
5994YOR229WWTM2Transcriptional modulatorTranscriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication
5995YOR230WWTM1Transcriptional modulatorTranscriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
5996YOR231C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
5997YOR231WMKK1MAPKK involved in the protein kinase C signaling pathwayMAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
5998YOR232WMGE1Mitochondrial matrix cochaperoneMitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress
5999YOR233WKIN4Serine/threonine protein kinaseSerine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
6000YOR234CRPL33BRibosomal 60S subunit protein L33BRibosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
6001YOR235WIRC13Putative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
6002YOR236WDFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesisDihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
6003YOR237WHES1Protein implicated in the regulation of ergosterol biosynthesisProtein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication
6004YOR238WYOR238WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
6005YOR239WABP140AdoMet-dependent tRNA methyltransferase and actin binding proteinAdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
6006YOR241WMET7Folylpolyglutamate synthetaseFolylpolyglutamate synthetase; catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA; protein abundance increases in response to DNA replication stress
6007YOR242CSSP2Sporulation specific protein that localizes to the spore wallSporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis
6008YOR243CPUS7Pseudouridine synthasePseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria
6009YOR244WESA1Catalytic subunit of the histone acetyltransferase complex (NuA4)Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases
6010YOR245CDGA1Diacylglycerol acyltransferaseDiacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
6011YOR246CENV9Protein proposed to be involved in vacuolar functionsProtein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
6012YOR247WSRL1Mannoprotein that exhibits a tight association with the cell wallMannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
6013YOR248WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6014YOR249CAPC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
6015YOR250CCLP1Component of the cleavage and polyadenylation factor I (CF I)Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation and in gene looping which affects reinitiation of transcription
6016YOR251CTUM1Rhodanese domain sulfur transferaseRhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized
6017YOR252WTMA16Protein of unknown function that associates with ribosomesProtein of unknown function that associates with ribosomes
6018YOR253WNAT5Subunit of protein N-terminal acetyltransferase NatASubunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
6019YOR254CSEC63Essential subunit of Sec63 complexEssential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, Sec66p and Sec72p
6020YOR255WOSW1Protein involved in sporulationProtein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p
6021YOR256CTRE2Transferrin receptor-like proteinTransferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
6022YOR257WCDC31Calcium-binding component of the spindle pole body (SPB) half-bridgeCalcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
6023YOR258WHNT3DNA 5' AMP hydrolase involved in DNA repairDNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
6024YOR259CRPT4ATPase of the 19S regulatory particle of the 26S proteasomeATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
6025YOR260WGCD1Gamma subunit of the translation initiation factor eIF2BGamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
6026YOR261CRPN8Essential non-ATPase regulatory subunit of the 26S proteasomeEssential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
6027YOR262WGPN2Putative GTPase with a role in biogenesis of RNA pol II and polIIIPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
6028YOR263CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W
6029YOR264WDSE3Daughter cell-specific protein, may help establish daughter fateDaughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
6030YOR265WRBL2Protein involved in microtubule morphogenesisProtein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress
6031YOR266WPNT1Mitochondrial integral inner membrane proteinMitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
6032YOR267CHRK1Protein kinaseProtein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
6033YOR268CYOR268CPutative protein of unknown functionPutative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
6034YOR269WPAC1Involved in nuclear migration, part of the dynein/dynactin pathwayInvolved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
6035YOR270CVPH1Subunit a of vacuolar-ATPase V0 domainSubunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress
6036YOR271CFSF1Putative proteinPutative protein; predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
6037YOR272WYTM1Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
6038YOR273CTPO4Polyamine transporter of the major facilitator superfamilyPolyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
6039YOR274WMOD5Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferaseDelta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects
6040YOR275CRIM20Protein involved in proteolytic activation of Rim101pProtein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
6041YOR276WCAF20Phosphoprotein of the mRNA cap-binding complexPhosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translation initiation; competes with eIF4G for binding to eIF4E
6042YOR277CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
6043YOR278WHEM4Uroporphyrinogen III synthaseUroporphyrinogen III synthase; catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
6044YOR279CRFM1Component of the Sum1p-Rfm1p-Hst1p complexComponent of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication
6045YOR280CFSH3Putative serine hydrolasePutative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
6046YOR281CPLP2Protein that interacts with the CCT complex to stimulate actin foldingProtein that interacts with the CCT complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP; CCT is short for chaperonin containing TCP-1; essential gene
6047YOR282WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
6048YOR283WYOR283WPhosphatase with a broad substrate specificityPhosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
6049YOR284WHUA2Cytoplasmic protein of unknown functionCytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
6050YOR285WRDL1Thiosulfate sulfurtransferaseThiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene
6051YOR286WRDL2Protein with rhodanese activityProtein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
6052YOR287CRRP36Component of 90S preribosomesComponent of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
6053YOR288CMPD1Member of the protein disulfide isomerase (PDI) familyMember of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
6054YOR289WYOR289WPutative protein of unknown functionPutative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
6055YOR290CSNF2Catalytic subunit of the SWI/SNF chromatin remodeling complexCatalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
6056YOR291WYPK9Vacuolar protein with a possible role in sequestering heavy metalsVacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
6057YOR292CYOR292CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
6058YOR293C-AYOR293C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
6059YOR293WRPS10AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
6060YOR294WRRS1Essential protein that binds ribosomal protein L11Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
6061YOR295WUAF30Subunit of UAF (upstream activation factor) complexSubunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
6062YOR296WYOR296WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene
6063YOR297CTIM18Component of the mitochondrial TIM22 complexComponent of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
6064YOR298C-AMBF1Transcriptional coactivatorTranscriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
6065YOR298WMUM3Protein of unknown function involved in outer spore wall organizationProtein of unknown function involved in outer spore wall organization; has similarity to the tafazzins superfamily of acyltransferases
6066YOR299WBUD7Member of the ChAPs family (Chs5p-Arf1p-binding proteins)Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
6067YOR300WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
6068YOR301WRAX1Protein involved in bud site selection during bipolar buddingProtein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
6069YOR302WYOR302WCPA1 uORFCPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA
6070YOR303WCPA1Small subunit of carbamoyl phosphate synthetaseSmall subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
6071YOR304C-ABIL1Protein that binds Bud6p and has a role in actin cable assemblyProtein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck
6072YOR304WISW2ATP-dependent DNA translocase involved in chromatin remodelingATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide
6073YOR305WRRG7Protein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
6074YOR306CMCH5Plasma membrane riboflavin transporterPlasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
6075YOR307CSLY41Protein involved in ER-to-Golgi transportProtein involved in ER-to-Golgi transport
6076YOR308CSNU66Component of the U4/U6.U5 snRNP complexComponent of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
6077YOR309CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
6078YOR310CNOP58Protein involved in producing mature rRNAs and snoRNAsProtein involved in producing mature rRNAs and snoRNAs; involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
6079YOR311CDGK1Diacylglycerol kinaseDiacylglycerol kinase; localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain
6080YOR312CRPL20BRibosomal 60S subunit protein L20BRibosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
6081YOR313CSPS4Protein whose expression is induced during sporulationProtein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
6082YOR314WYOR314WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6083YOR314W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6084YOR315WSFG1Nuclear protein putative transcription factorNuclear protein putative transcription factor; required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
6085YOR316CCOT1Vacuolar transporter that mediates zinc transport into the vacuoleVacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
6086YOR316C-AYOR316C-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
6087YOR317WFAA1Long chain fatty acyl-CoA synthetaseLong chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
6088YOR318CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
6089YOR319WHSH49U2-snRNP associated splicing factorU2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
6090YOR320CGNT1N-acetylglucosaminyltransferaseN-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus
6091YOR321WPMT3Protein O-mannosyltransferaseProtein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
6092YOR322CLDB19Alpha-arrestin involved in ubiquitin-dependent endocytosisAlpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
6093YOR323CPRO2Gamma-glutamyl phosphate reductaseGamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis
6094YOR324CFRT1Tail-anchored ER membrane protein of unknown functionTail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
6095YOR325WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1
6096YOR326WMYO2Type V myosin motor involved in actin-based transport of cargosType V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
6097YOR327CSNC2Vesicle membrane receptor protein (v-SNARE)Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; Snc2p levels regulated by Vps45p; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication
6098YOR328WPDR10ATP-binding cassette (ABC) transporterATP-binding cassette (ABC) transporter; multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p
6099YOR329CSCD5Protein required for normal actin organization and endocytosisProtein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
6100YOR329W-ADubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR
6101YOR330CMIP1Mitochondrial DNA polymerase gammaMitochondrial DNA polymerase gamma subunit; conserved C-terminal segment is required for the maintenance of mitochondrial genome; its human ortholog, POLG, can rescue mip1 phenotypes, and mutations in POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
6102YOR331CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
6103YOR332WVMA4Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase)Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
6104YOR333CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of MRS2 gene required for respiratory growth
6105YOR334WMRS2Mitochondrial inner membrane Mg(2+) channelMitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA
6106YOR335CALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetaseCytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
6107YOR335W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ALA1/YOR335C
6108YOR336WKRE5Protein required for beta-1,6 glucan biosynthesisProtein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
6109YOR337WTEA1Ty1 enhancer activator involved in Ty enhancer-mediated transcriptionTy1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
6110YOR338WYOR338WPutative protein of unknown functionPutative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication
6111YOR339CUBC11Ubiquitin-conjugating enzymeUbiquitin-conjugating enzyme; most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
6112YOR340CRPA43RNA polymerase I subunit A43RNA polymerase I subunit A43
6113YOR341WRPA190RNA polymerase I largest subunit A190RNA polymerase I largest subunit A190
6114YOR342CYOR342CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
6115YOR343CYOR343CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6116YOR343W-AYOR343W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
6117YOR343W-BYOR343W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
6118YOR344CTYE7Serine-rich protein that contains a bHLH DNA binding motifSerine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix
6119YOR345CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
6120YOR346WREV1Deoxycytidyl transferaseDeoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
6121YOR347CPYK2Pyruvate kinasePyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication
6122YOR348CPUT4Proline permeaseProline permease; required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
6123YOR349WCIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) foldingTubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
6124YOR350CMNE1Protein involved in splicing Group I aI5-beta intron from COX1 mRNAProtein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein
6125YOR351CMEK1Meiosis-specific serine/threonine protein kinaseMeiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis
6126YOR352WTFB6Subunit of TFIIH complexSubunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
6127YOR353CSOG2Key component of the RAM signaling networkKey component of the RAM signaling network; required for proper cell morphogenesis and cell separation after mitosis
6128YOR354CMSC6Multicopy suppressor of HER2 involved in mitochondrial translationProtein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6129YOR355WGDS1Protein of unknown functionProtein of unknown function; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6130YOR356WCIR2Putative ortholog of human ETF-dHPutative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase
6131YOR357CSNX3Sorting nexin for late-Golgi enzymesSorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
6132YOR358WHAP5Subunit of the Hap2p/3p/4p/5p CCAAT-binding complexSubunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
6133YOR359WVTS1Flap-structured DNA-binding and RNA-binding proteinFlap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity
6134YOR360CPDE2High-affinity cyclic AMP phosphodiesteraseHigh-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
6135YOR361CPRT1eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3)eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
6136YOR362CPRE10Alpha 7 subunit of the 20S proteasomeAlpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
6137YOR363CPIP2Autoregulatory, oleate-activated transcription factorAutoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
6138YOR364WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
6139YOR365CYOR365CPutative protein of unknown functionPutative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
6140YOR366WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
6141YOR367WSCP1Component of yeast cortical actin cytoskeletonComponent of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
6142YOR368WRAD17Checkpoint proteinCheckpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins
6143YOR369CRPS12Protein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog
6144YOR370CMRS6Rab escort proteinRab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
6145YOR371CGPB1Multistep regulator of cAMP-PKA signalingMultistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
6146YOR372CNDD1Transcriptional activator essential for nuclear divisionTranscriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
6147YOR373WNUD1Component of the spindle pole body outer plaqueComponent of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance
6148YOR374WALD4Mitochondrial aldehyde dehydrogenaseMitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol
6149YOR375CGDH1NADP(+)-dependent glutamate dehydrogenaseNADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
6150YOR376WYOR376WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene
6151YOR376W-AYOR376W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
6152YOR377WATF1Alcohol acetyltransferaseAlcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
6153YOR378WAMF1Low affinity NH4+ transporterPutative paralog of ATR1; but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene
6154YOR379CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W
6155YOR380WRDR1Transcriptional repressor involved in regulating multidrug resistanceTranscriptional repressor involved in regulating multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins
6156YOR381WFRE3Ferric reductaseFerric reductase; reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
6157YOR381W-AYOR381W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
6158YOR382WFIT2Mannoprotein that is incorporated into the cell wallMannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall
6159YOR383CFIT3Mannoprotein that is incorporated into the cell wallMannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall
6160YOR384WFRE5Putative ferric reductase with similarity to Fre2pPutative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6161YOR385WYOR385WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
6162YOR386WPHR1DNA photolyase involved in photoreactivationDNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p
6163YOR387CYOR387CPutative protein of unknown functionPutative protein of unknown function; regulated by the metal-responsive Aft1p transcription factor; highly inducible in zinc-depleted conditions; localizes to the soluble fraction; YOR387C has a paralog, VEL1, that arose from a single-locus duplication
6164YOR388CFDH1NAD(+)-dependent formate dehydrogenaseNAD(+)-dependent formate dehydrogenase; may protect cells from exogenous formate
6165YOR389WYOR389WPutative protein of unknown functionPutative protein of unknown function; expression regulated by copper levels
6166YOR390WFEX1Protein involved in fluoride exportProtein involved in fluoride export; nearly identical to FEX2, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins
6167YOR391CHSP33Possible chaperone and cysteine proteasePossible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer
6168YOR392WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; gene expression induced by heat
6169YOR393WERR1Putative phosphopyruvate hydrataseProtein of unknown function; has similarity to enolases
6170YOR394C-AYOR394C-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6171YOR394WPAU21Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau22p; encodes 2 proteins that are translated from 2 different start codons
6172YOR396C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6173YOR396WYRF1-8One of several telomeric Y' element-encoded DNA helicasesOne of several telomeric Y' element-encoded DNA helicases; known as Y'-Help1 (Y'-HELicase Protein 1)
6174YPL001WHAT1Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complexCatalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex; uses the cofactor acetyl coenzyme A to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
6175YPL002CSNF8Component of the ESCRT-II complexComponent of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
6176YPL003WULA1Protein that activates Rub1p (NEDD8) before neddylationProtein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation
6177YPL004CLSP1Eisosome core componentEisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
6178YPL005WAEP3Peripheral mitochondrial inner membrane proteinPeripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
6179YPL006WNCR1Vacuolar membrane proteinVacuolar membrane protein; transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to the accumulation of high amounts of lipids observed in patients with Neimann-Pick C, a disease caused by loss-of-function mutations in NPC1, the functional ortholog of Ncr1p
6180YPL007CTFC8Subunit of RNA polymerase III transcription initiation factor complexSubunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
6181YPL008WCHL1Probable DNA helicaseProbable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
6182YPL009CRQC2Component of RQC, which mediates nascent chain degradationComponent of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation involved in protein translation; monitors translation stress and signals this to Hsf1p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
6183YPL010WRET3Zeta subunit of the coatomer complex (COPI)Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
6184YPL011CTAF3TFIID subunit (47 kDa)TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation
6185YPL012WRRP12Protein required for export of the ribosomal subunitsProtein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; also plays a role in the cell cycle and the DNA damage response; contains HEAT-repeats
6186YPL013CMRPS16Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
6187YPL014WCIP1Cyclin-dependent kinase inhibitorPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
6188YPL015CHST2Cytoplasmic NAD(+)-dependent protein deacetylaseCytoplasmic NAD(+)-dependent protein deacetylase; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
6189YPL016WSWI1Subunit of the SWI/SNF chromatin remodeling complexSubunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
6190YPL017CIRC15Microtubule associated proteinMicrotubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication
6191YPL018WCTF19Outer kinetochore protein, needed for accurate chromosome segregationOuter kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2
6192YPL019CVTC3Regulatory subunit of the vacuolar transporter chaperone (VTC) complexSubunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication
6193YPL020CULP1Protease that specifically cleaves Smt3p protein conjugatesProtease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
6194YPL021WECM23Non-essential protein of unconfirmed functionNon-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
6195YPL022WRAD1Single-stranded DNA endonuclease (with Rad10p)Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
6196YPL023CMET12Protein with MTHFR activity in vitroProtein with MTHFR activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of methylenetetrahydrofolate reductase (MTHFR)
6197YPL024WRMI1Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complexSubunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase
6198YPL025CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6199YPL026CSKS1Putative serine/threonine protein kinasePutative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication
6200YPL027WSMA1Protein of unknown function involved in prospore membrane assemblyProtein of unknown function involved in prospore membrane assembly; involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p
6201YPL028WERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase)Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
6202YPL029WSUV3ATP-dependent RNA helicaseATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
6203YPL030WTRM44tRNA(Ser) Um(44) 2'-O-methyltransferasetRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
6204YPL031CPHO85Cyclin-dependent kinaseCyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle
6205YPL032CSVL3Protein of unknown functionProtein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication
6206YPL033CSRL4Protein of unknown functionProtein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p
6207YPL034WYPL034WPutative protein of unknown functionPutative protein of unknown function; YPL034W is not essential gene
6208YPL035CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
6209YPL036WPMA2Plasma membrane H+-ATPasePlasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
6210YPL037CEGD1Subunit beta1 of the nascent polypeptide-associated complex (NAC)Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication
6211YPL038WMET31Zinc-finger DNA-binding transcription factorZinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication
6212YPL038W-AYPL038W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
6213YPL039WYPL039WPutative protein of unknown functionPutative protein of unknown function; YPL039W is not an essential gene
6214YPL040CISM1Mitochondrial isoleucyl-tRNA synthetaseMitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth
6215YPL041CMRX11Protein that associates with mitochondrial ribosomeProtein of unknown function involved in maintenance of telomere length
6216YPL042CSSN3Cyclin-dependent protein kinaseCyclin-dependent protein kinase; component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
6217YPL043WNOP4Nucleolar proteinNucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
6218YPL044CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
6219YPL045WVPS16Subunit of the HOPS and the CORVET complexesSubunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages
6220YPL046CELC1Elongin C, conserved among eukaryotesElongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair
6221YPL047WSGF11Integral subunit of SAGA histone acetyltransferase complexIntegral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
6222YPL048WCAM1One of two isoforms of the gamma subunit of eEF1BNuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma
6223YPL049CDIG1MAP kinase-responsive inhibitor of the Ste12p transcription factorMAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication
6224YPL050CMNN9Subunit of Golgi mannosyltransferase complexSubunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p
6225YPL051WARL3ARF-like small GTPase of the RAS superfamilyARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1
6226YPL052WOAZ1Regulator of ornithine decarboxylase Spe1pRegulator of ornithine decarboxylase Spe1p; antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p
6227YPL053CKTR6Probable mannosylphosphate transferaseProbable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
6228YPL054WLEE1Zinc-finger protein of unknown functionZinc-finger protein of unknown function
6229YPL055CLGE1Protein of unknown functionProtein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
6230YPL056CLCL1Putative protein of unknown functionPutative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
6231YPL057CSUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunitMannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
6232YPL058CPDR12Plasma membrane ATP-binding cassette (ABC) transporterPlasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
6233YPL059WGRX5Glutathione-dependent oxidoreductaseGlutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p
6234YPL060WMFM1Mitochondrial inner membrane magnesium transporterMitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
6235YPL061WALD6Cytosolic aldehyde dehydrogenaseCytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
6236YPL062WYPL062WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
6237YPL063WTIM50Essential component of the TIM23 complexEssential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
6238YPL064CCWC27Component of a complex containing Cef1pComponent of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
6239YPL065WVPS28Component of the ESCRT-I complexComponent of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p
6240YPL066WRGL1Regulator of Rho1p signaling, cofactor of Tus1pRegulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
6241YPL067CHTC1Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
6242YPL068CYPL068CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
6243YPL069CBTS1Geranylgeranyl diphosphate synthase (GGPS)Geranylgeranyl diphosphate synthase; increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
6244YPL070WMUK1Guanine nucleotide exchange factor (GEF)Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain
6245YPL071CYPL071CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
6246YPL072WUBP16Deubiquitinating enzyme anchored to the outer mitochondrial membraneDeubiquitinating enzyme anchored to the outer mitochondrial membrane; probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria
6247YPL073CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments
6248YPL074WYTA6Putative ATPase of the CDC48/PAS1/SEC18 (AAA) familyPutative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
6249YPL075WGCR1Transcriptional activator of genes involved in glycolysisTranscriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
6250YPL076WGPI2Protein involved in the synthesis of GlcNAc-PIProtein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
6251YPL077CYPL077CPutative protein of unknown functionPutative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
6252YPL078CATP4Subunit b of the stator stalk of mitochondrial F1F0 ATP synthaseSubunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated
6253YPL079WRPL21BRibosomal 60S subunit protein L21BRibosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
6254YPL080CYPL080CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6255YPL081WRPS9AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
6256YPL082CMOT1Essential protein involved in regulation of transcriptionEssential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
6257YPL083CSEN54Subunit of the tRNA splicing endonucleaseSubunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p
6258YPL084WBRO1Cytoplasmic class E vacuolar protein sorting (VPS) factorCytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
6259YPL085WSEC16COPII vesicle coat protein required for ER transport vesicle buddingCOPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
6260YPL086CELP3Subunit of Elongator complexSubunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin
6261YPL087WYDC1Alkaline dihydroceramidase, involved in sphingolipid metabolismAlkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
6262YPL088WYPL088WPutative aryl alcohol dehydrogenasePutative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
6263YPL089CRLM1MADS-box transcription factorMADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
6264YPL090CRPS6AProtein component of the small (40S) ribosomal subunitProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
6265YPL091WGLR1Cytosolic and mitochondrial glutathione oxidoreductaseCytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
6266YPL092WSSU1Plasma membrane sulfite pump involved in sulfite metabolismPlasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein
6267YPL093WNOG1Putative GTPasePutative GTPase; associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
6268YPL094CSEC62Essential subunit of Sec63 complexEssential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec62p, Sec66p and Sec72p
6269YPL095CEEB1Acyl-coenzymeA:ethanol O-acyltransferaseAcyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication
6270YPL096C-AERI1Endoplasmic reticulum membrane protein that binds and inhibits Ras2pEndoplasmic reticulum membrane protein that binds and inhibits Ras2p; binds to and inhibits GTP-bound Ras2p at the endoplasmic reticulum (ER); component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
6271YPL096WPNG1Conserved peptide N-glycanaseConserved peptide N-glycanase; required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p
6272YPL097WMSY1Mitochondrial tyrosyl-tRNA synthetaseMitochondrial tyrosyl-tRNA synthetase
6273YPL098CMGR2Subunit of the TIM23 translocase complexSubunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
6274YPL099CINA17F1F0 ATPase synthase peripheral stalk assembly factorF1F0 ATPase synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Ina22p and ATP synthase subunits; null mutant displays elevated frequency of mitochondrial genome loss and has a respiratory growth defect
6275YPL100WATG21Phosphoinositide binding proteinPhosphoinositide binding protein; required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein
6276YPL101WELP4Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplexSubunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
6277YPL102CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
6278YPL103CFMP30Protein with a role in maintaining mitochondrial morphologyProtein with a role in maintaining mitochondrial morphology; also involved in maintaining normal cardiolipin levels; mitochondrial inner membrane protein; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
6279YPL104WMSD1Mitochondrial aspartyl-tRNA synthetaseMitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
6280YPL105CSYH1Protein of unknown function that influences nuclear pore distributionProtein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
6281YPL106CSSE1ATPase component of heat shock protein Hsp90 chaperone complexATPase component of heat shock protein Hsp90 chaperone complex; plays a role in determining prion variants; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
6282YPL107WDPC25Putative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene
6283YPL108WYPL108WCytoplasmic protein of unknown functionCytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
6284YPL109CMCO76UbiB family proteinPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6285YPL110CGDE1Glycerophosphocholine (GroPCho) phosphodiesteraseGlycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
6286YPL111WCAR1Arginase, catabolizes arginine to ornithine and ureaArginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance
6287YPL112CPEX25Peripheral peroxisomal membrane peroxinPeripheral peroxisomal membrane peroxin; required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with Pex27p; PEX25 has a paralog, PEX27, that arose from the whole genome duplication
6288YPL113CYPL113CGlyoxylate reductaseGlyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
6289YPL114WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
6290YPL115CBEM3Rho GTPase activating protein (RhoGAP)Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
6291YPL116WHOS3Trichostatin A-insensitive homodimeric histone deacetylase (HDAC)Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
6292YPL117CIDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomeraseIsopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; isopentenyl diphosphate:dimethylallyl diphosphate isomerase is also known as IPP isomerase
6293YPL118WMRP51Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
6294YPL119CDBP1Putative ATP-dependent RNA helicase of the DEAD-box protein familyPutative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication
6295YPL119C-AYPL119C-APutative protein of unknown functionPutative protein of unknown function; identified by expression profiling and mass spectrometry
6296YPL120WVPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and IISubunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has a novel globular fold that is essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of the higher eukaryotic gene Beclin 1
6297YPL121CMEI5Meiosis-specific protein involved in meiotic recombinationMeiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
6298YPL122CTFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexesSubunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
6299YPL123CRNY1Vacuolar RNase of the T(2) familyVacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
6300YPL124WSPC29Inner plaque spindle pole body (SPB) componentInner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
6301YPL125WKAP120Karyopherin responsible for the nuclear import of Rpf1pKaryopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor
6302YPL126WNAN1U3 snoRNP proteinU3 snoRNP protein; component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
6303YPL127CHHO1Histone H1, linker histone with roles in meiosis and sporulationHistone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination
6304YPL128CTBF1Telobox-containing general regulatory factorTelobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
6305YPL129WTAF14Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexesSubunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain
6306YPL130WSPO19Meiosis-specific prospore proteinMeiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation; SPO19 has a paralog, YOR214C, that arose from the whole genome duplication
6307YPL131WRPL5Ribosomal 60S subunit protein L5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
6308YPL132WCOX11Protein required for delivery of copper to Cox1pProtein required for delivery of copper to Cox1p; mitochondrial inner membrane protein; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
6309YPL133CRDS2Transcription factor involved in regulating gluconeogenesisTranscription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
6310YPL134CODC1Mitochondrial inner membrane transporterMitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation; ODC1 has a paralog, ODC2, that arose from the whole genome duplication
6311YPL135C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6312YPL135WISU1Conserved protein of the mitochondrial matrixConserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable; ISU1 has a paralog, ISU2, that arose from the whole genome duplication
6313YPL136WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C
6314YPL137CGIP3Cytoplasmic protein that regulates protein phosphatase 1 Glc7pCytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
6315YPL138CSPP1Subunit of COMPASS (Set1C)Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia
6316YPL139CUME1Component of both the Rpd3S and Rpd3L histone deacetylase complexesComponent of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication
6317YPL140CMKK2MAPKK involved in the protein kinase C signaling pathwayMAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
6318YPL141CFRK1Protein kinase of unknown cellular roleProtein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
6319YPL142CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
6320YPL143WRPL33ARibosomal 60S subunit protein L33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
6321YPL144WPOC4Component of a heterodimeric Poc4p-Irc25p chaperoneComponent of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
6322YPL145CKES1One of seven members of the yeast oxysterol binding protein familyOne of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
6323YPL146CNOP53Nucleolar proteinNucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p and with the nucleolar proteins Nop17p and Nip7p; null mutant is viable but growth is severely impaired
6324YPL147WPXA1Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2pSubunit of a heterodimeric peroxisomal ABC transport complex; required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; complex also includes Pxa2p
6325YPL148CPPT2Phosphopantetheine:protein transferase (PPTase)Phosphopantetheine:protein transferase (PPTase); activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
6326YPL149WATG5Conserved protein involved in autophagy and the Cvt pathwayConserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; also involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
6327YPL150WYPL150WProtein kinase of unknown cellular roleProtein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
6328YPL151CPRP46Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
6329YPL152WRRD2Peptidyl-prolyl cis/trans-isomerasePeptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
6330YPL152W-AYPL152W-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6331YPL153CRAD53DNA damage response protein kinaseDNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia
6332YPL154CPEP4Vacuolar aspartyl protease (proteinase A)Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates
6333YPL155CKIP2Kinesin-related motor protein involved in mitotic spindle positioningKinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle
6334YPL156CPRM4Pheromone-regulated protein proposed to be involved in matingPheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift
6335YPL157WTGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferaseTrimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p
6336YPL158CAIM44Protein that regulates Cdc42p and Rho1pProtein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
6337YPL159CPET20Mitochondrial proteinMitochondrial protein; required for respiratory growth under some conditions and for stability of the mitochondrial genome
6338YPL160WCDC60Cytosolic leucyl tRNA synthetaseCytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA
6339YPL161CBEM4Protein involved in establishment of cell polarity and bud emergenceProtein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
6340YPL162CYPL162CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
6341YPL163CSVS1Cell wall and vacuolar proteinCell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
6342YPL164CMLH3Protein involved in DNA mismatch repair and meiotic recombinationProtein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
6343YPL165CSET6SET domain protein of unknown functionSET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
6344YPL166WATG29Autophagy-specific proteinAutophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
6345YPL167CREV3Catalytic subunit of DNA polymerase zetaCatalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p
6346YPL168WMRX4Protein that associates with mitochondrial ribosomePutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
6347YPL169CMEX67Poly(A)RNA binding protein involved in nuclear mRNA exportPoly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore; ortholog of human TAP
6348YPL170WDAP1Heme-binding proteinHeme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
6349YPL171COYE3Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN)Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
6350YPL172CCOX10Heme A:farnesyltransferaseHeme A:farnesyltransferase; catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
6351YPL173WMRPL40Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
6352YPL174CNIP100Large subunit of the dynactin complexLarge subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
6353YPL175WSPT14UDP-glycosyltransferase subunit of the GPI-GnT complexUDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
6354YPL176CTRE1Transferrin receptor-like proteinTransferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
6355YPL177CCUP9Homeodomain-containing transcriptional repressorHomeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
6356YPL178WCBC2Small subunit of the heterodimeric cap binding complex with Sto1pSmall subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
6357YPL179WPPQ1Protein phosphatase that regulates the mating responseProtein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family
6358YPL180WTCO89Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p)Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
6359YPL181WCTI6Component of the Rpd3L histone deacetylase complexComponent of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
6360YPL182CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
6361YPL183CRTT10WD40 domain-containing protein involved in endosomal recyclingWD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
6362YPL183W-ARTC6Protein involved in translationProtein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
6363YPL184CMRN1RNA-binding protein that may be involved in translational regulationRNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
6364YPL185WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C
6365YPL186CUIP4Protein that interacts with Ulp1pProtein that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope
6366YPL187WMF(ALPHA)1Mating pheromone alpha-factor, made by alpha cellsMating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor; MF(ALPHA)1 has a paralog, MF(ALPHA)2, that arose from the whole genome duplication
6367YPL188WPOS5Mitochondrial NADH kinaseMitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
6368YPL189C-ACOA2Cytochrome oxidase assembly factorCytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
6369YPL189WGUP2Probable membrane proteinProbable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; GUP2 has a paralog, GUP1, that arose from the whole genome duplication
6370YPL190CNAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p)RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability
6371YPL191CYPL191CK48-specific deubiquitinating (DUB) enzymePutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication
6372YPL192CPRM3Protein required for nuclear envelope fusion during karyogamyProtein required for nuclear envelope fusion during karyogamy; pheromone-regulated; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
6373YPL193WRSA1Protein involved in the assembly of 60S ribosomal subunitsProtein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
6374YPL194WDDC1DNA damage checkpoint proteinDNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
6375YPL195WAPL5Delta adaptin-like subunit of the clathrin associated protein complexDelta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3
6376YPL196WOXR1Protein of unknown function required for oxidative damage resistanceProtein of unknown function required for oxidative damage resistance; required for normal levels of resistance to oxidative damage; null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
6377YPL197CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
6378YPL198WRPL7BRibosomal 60S subunit protein L7BRibosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
6379YPL199CYPL199CPutative protein of unknown functionPutative protein of unknown function; predicted to be palmitoylated
6380YPL200WCSM4Protein required for accurate chromosome segregation during meiosisProtein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements; functions with meiosis-specific telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2, that arose from the whole genome duplication
6381YPL201CYIG1Protein that interacts with glycerol 3-phosphataseProtein that interacts with glycerol 3-phosphatase; plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
6382YPL202CAFT2Iron-regulated transcriptional activatorIron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
6383YPL203WTPK2cAMP-dependent protein kinase catalytic subunitcAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia
6384YPL204WHRR25Conserved casein kinaseProtein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta)
6385YPL205CHypothetical protein; deletion of locus affects telomere length
6386YPL206CPGC1Phosphatidylglycerol phospholipase CPhosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs
6387YPL207WTYW1Iron-sulfer protein required for synthesis of Wybutosine modified tRNAIron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
6388YPL208WRKM1SET-domain lysine-N-methyltransferaseSET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp)
6389YPL209CIPL1Aurora kinase of chromosomal passenger complexAurora kinase of conserved chromosomal passenger complex; mediates release on mono-oriented kinetochores from microtubules in meiosis I, also release of kinetochores from cluster at SPBs at meiosis exit; helps maintain condensed chromosomes during anaphase, early telophase; required for SPB cohesion and prevention of multipolar spindle formation; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest
6390YPL210CSRP72Core component of the signal recognition particle (SRP)Core component of the signal recognition particle (SRP); the SRP is a ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
6391YPL211WNIP7Nucleolar protein required for 60S ribosome subunit biogenesisNucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
6392YPL212CPUS1tRNA:pseudouridine synthasetRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
6393YPL213WLEA1Component of U2 snRNP complexComponent of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
6394YPL214CTHI6Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinaseThiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase; required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
6395YPL215WCBP3Mitochondrial protein required for assembly of cytochrome bc1 complexMitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
6396YPL216WYPL216WPutative protein of unknown functionPutative protein of unknown function; not an essential gene; YPL216W has a paralog, ITC1, that arose from the whole genome duplication
6397YPL217CBMS1GTPase required for ribosomal subunit synthesis and rRNA processingGTPase required for ribosomal subunit synthesis and rRNA processing; required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
6398YPL218WSAR1ARF family GTPaseGTPase, GTP-binding protein of the ARF family; component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity helping in vesicle formation
6399YPL219WPCL8CyclinCyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication
6400YPL220WRPL1ARibosomal 60S subunit protein L1ARibosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
6401YPL221WFLC1Flavin adenine dinucleotide transporterPutative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication
6402YPL222C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6403YPL222WFMP40Putative protein of unknown functionPutative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6404YPL223CGRE1Hydrophilin essential in desiccation-rehydration processHydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication
6405YPL224CMMT2Putative metal transporter involved in mitochondrial iron accumulationPutative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication
6406YPL225WYPL225WProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
6407YPL226WNEW1ATP binding cassette proteinATP binding cassette protein; cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
6408YPL227CALG5UDP-glucose:dolichyl-phosphate glucosyltransferaseUDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum
6409YPL228WCET1RNA 5'-triphosphatase involved in mRNA 5' cappingRNA 5'-triphosphatase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of a Cet1p homodimer and two molecules of the guanylyltransferase Ceg1p; Cet1p also has a role in regulation of RNA pol II pausing at promoter-proximal sites; interaction between Cet1p and Ceg1p is required for Ceg1p nuclear import; mammalian enzyme is a single bifunctional polypeptide; CET1 has a paralog, CTL1, that arose from the whole genome duplication
6410YPL229WYPL229WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
6411YPL230WUSV1Putative transcription factor containing a C2H2 zinc fingerPutative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication
6412YPL231WFAS2Alpha subunit of fatty acid synthetaseAlpha subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
6413YPL232WSSO1Plasma membrane t-SNAREPlasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication
6414YPL233WNSL1Essential component of the MIND kinetochore complexEssential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
6415YPL234CVMA11Vacuolar ATPase V0 domain subunit c'Vacuolar ATPase V0 domain subunit c'; involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
6416YPL235WRVB2ATP-dependent DNA helicase, also known as reptinATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
6417YPL236CENV7Vacuolar membrane protein kinaseVacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
6418YPL237WSUI3Beta subunit of the translation initiation factor eIF2Beta subunit of the translation initiation factor eIF2; involved in the identification of the start codon; proposed to be involved in mRNA binding
6419YPL238CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
6420YPL239WYAR1Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperoneAnkyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
6421YPL240CHSP82Hsp90 chaperoneHsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
6422YPL241CCIN2GTPase-activating protein (GAP) for Cin4pGTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
6423YPL242CIQG1Essential protein required for determination of budding patternEssential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
6424YPL243WSRP68Core component of the signal recognition particle (SRP) complexCore component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
6425YPL244CHUT1Protein with a role in UDP-galactose transport to the Golgi lumenProtein with a role in UDP-galactose transport to the Golgi lumen; has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
6426YPL245WYPL245WPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
6427YPL246CRBD2Possible rhomboid proteasePossible rhomboid protease; has similarity to eukaryotic rhomboid proteases including Pcp1p
6428YPL247CYPL247CPutative protein of unknown functionPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
6429YPL248CGAL4DNA-binding transcription factor required for activating GAL genesDNA-binding transcription factor required for activating GAL genes; responds to galactose; repressed by Gal80p and activated by Gal3p
6430YPL249CGYP5GTPase-activating protein (GAP) for yeast Rab family membersGTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication
6431YPL249C-ARPL36BRibosomal 60S subunit protein L36BRibosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
6432YPL250CATG41Protein of unknown functionProtein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ICY2 has a paralog, ICY1, that arose from the whole genome duplication
6433YPL250W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified ORF RPL36B/YPL249C-A; identified by fungal homology and RT-PCR
6434YPL251WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
6435YPL252CYAH1Ferredoxin of the mitochondrial matrixFerredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
6436YPL253CVIK1Protein that forms a kinesin-14 heterodimeric motor with Kar3pProtein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication
6437YPL254WHFI1Adaptor protein required for structural integrity of the SAGA complexAdaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
6438YPL255WBBP1Protein required for the spindle pole body (SPB) duplicationProtein required for the spindle pole body (SPB) duplication; localizes at the cytoplasmic side of the central plaque periphery of the SPB; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
6439YPL256CCLN2G1 cyclin involved in regulation of the cell cycleG1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication
6440YPL257WYPL257WPutative protein of unknown functionPutative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
6441YPL257W-AYPL257W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
6442YPL257W-BYPL257W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
6443YPL258CTHI21Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinaseHydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
6444YPL259CAPM1Mu1-like medium subunit of the AP-1 complexMu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex
6445YPL260WCUB1Conserved fungal gene linked to DNA repair and proteasome functionPutative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
6446YPL261CYPL261CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YPL260W
6447YPL262WFUM1FumaraseFumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
6448YPL263CKEL3Cytoplasmic protein of unknown functionCytoplasmic protein of unknown function
6449YPL264CYPL264CPutative membrane protein of unknown functionPutative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
6450YPL265WDIP5Dicarboxylic amino acid permeaseDicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
6451YPL266WDIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase)Essential 18S rRNA dimethylase (dimethyladenosine transferase); responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
6452YPL267WACM1Pseudosubstrate inhibitor of the APC/CPseudosubstrate inhibitor of the APC/C; suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein; the anaphase-promoting complex/cyclosome is also known as APC/C
6453YPL268WPLC1Phospholipase CPhospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation
6454YPL269WKAR9Spindle positioning factorKaryogamy protein; required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules; localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
6455YPL270WMDL2Mitochondrial inner membrane half-type ABC transporterMitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
6456YPL271WATP15Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthaseEpsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
6457YPL272CPBI1Putative protein of unknown functionPutative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene
6458YPL273WSAM4S-adenosylmethionine-homocysteine methyltransferaseS-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
6459YPL274WSAM3High-affinity S-adenosylmethionine permeaseHigh-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
6460YPL277CYPL277CPutative protein of unknown functionPutative protein of unknown function; localized to the membranes; gene expression regulated by copper levels
6461YPL278CYPL278CPutative protein of unknown functionPutative protein of unknown function; gene expression regulated by copper levels
6462YPL279CFEX2Protein involved in fluoride exportProtein involved in fluoride export; nearly identical to FEX1, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins
6463YPL280WHSP32Possible chaperone and cysteine proteasePossible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer
6464YPL281CERR2Enolase, a phosphopyruvate hydrataseEnolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant
6465YPL282CPAU22Protein of unknown functionProtein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau21p; encodes 2 proteins that are translated from 2 different start codons
6466YPL283CYRF1-7Helicase encoded by the Y' element of subtelomeric regionsHelicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
6467YPL283W-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6468YPL283W-BProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6469YPR001WCIT3Dual specificity mitochondrial citrate and methylcitrate synthaseDual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
6470YPR002C-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the Ty1 long terminal repeat, YPRWdelta12
6471YPR002WPDH1Putative 2-methylcitrate dehydratasePutative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
6472YPR003CYPR003CPutative sulfate permeasePutative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene
6473YPR004CAIM45Putative ortholog of mammalian ETF-alphaPutative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit
6474YPR005CHAL1Cytoplasmic protein involved in halotoleranceCytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p
6475YPR006CICL22-methylisocitrate lyase of the mitochondrial matrix2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
6476YPR007CREC8Meiosis-specific component of the sister chromatid cohesion complexMeiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; independent of its role in sister chromatid cohesion, Rec8p promotes allelic collisions and prevents nonspecific chromosome interactions; homolog of S. pombe Rec8p
6477YPR008WHAA1Transcriptional activator involved in adaptation to weak acid stressTranscriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
6478YPR009WSUT2Zn2Cys6 family transcription factorPutative transcription factor of the Zn2Cys6 family; regulates sterol uptake under anaerobic conditions along with SUT1; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; positively regulates mating along with SUT1 by repressing the expression of genes (PRR2, NCE102 and RHO5) which function as mating inhibitors; SUT2 has a paralog, SUT1, that arose from the whole genome duplication
6479YPR010CRPA135RNA polymerase I second largest subunit A135RNA polymerase I second largest subunit A135
6480YPR010C-AMIN8Putative protein of unknown functionPutative protein of unknown function; conserved among Saccharomyces sensu stricto species
6481YPR011CYPR011CMitochondrial transporterMitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
6482YPR012WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
6483YPR013CCMR3Putative zinc finger proteinPutative zinc finger protein; YPR013C is not an essential gene
6484YPR014CYPR014CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
6485YPR015CYPR015CPutative zinc finger transcription factorPutative protein of unknown function; overexpression causes a cell cycle delay or arrest
6486YPR016CTIF6Constituent of 66S pre-ribosomal particlesConstituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
6487YPR016W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6488YPR017CDSS4Guanine nucleotide dissociation stimulator for Sec4pGuanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
6489YPR018WRLF2Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1)Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription
6490YPR019WMCM4Essential helicase component of heterohexameric MCM2-7 complexesEssential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
6491YPR020WATP20Subunit g of the mitochondrial F1F0 ATP synthaseSubunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae
6492YPR021CAGC1Mitochondrial amino acid transporterMitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
6493YPR022CSDD4Putative transcription factor, as suggested by computational analysisPutative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
6494YPR023CEAF3Component of the Rpd3S histone deacetylase complexComponent of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
6495YPR024WYME1Catalytic subunit of i-AAA protease complexCatalytic subunit of the i-AAA protease complex; complex is located in the mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as a nonconventional translocation motor to pull PNPase into the intermembrane space; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
6496YPR025CCCL1Cyclin associated with protein kinase Kin28pCyclin associated with protein kinase Kin28p; Kin28p is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
6497YPR026WATH1Acid trehalase required for utilization of extracellular trehaloseAcid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress
6498YPR027CYPR027CPutative protein of unknown functionPutative protein of unknown function
6499YPR028WYOP1Reticulon-interacting proteinMembrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
6500YPR029CAPL4Gamma-adaptinGamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
6501YPR030WCSR2Nuclear ubiquitin protein ligase binding proteinNuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication
6502YPR031WNTO1Subunit of the NuA3 histone acetyltransferase complexSubunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
6503YPR032WSRO7Effector of Rab GTPase Sec4pEffector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
6504YPR033CHTS1Cytoplasmic and mitochondrial histidine tRNA synthetaseCytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
6505YPR034WARP7Component of both the SWI/SNF and RSC chromatin remodeling complexesComponent of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
6506YPR035WGLN1Glutamine synthetase (GS)Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; forms filaments of back-to-back stacks of cylindrical homo-decamers at low pH, leading to enzymatic inactivation and storage during states of advanced cellular starvation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
6507YPR036WVMA13Subunit H of the V1 peripheral membrane domain of V-ATPaseSubunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
6508YPR036W-ASPO24Small (67 amino acids) protein involved in sporulationSmall (67 amino acids) protein involved in sporulation; localizes to the prospore membrane; phosphorylated during meiosis; a longer, 5'-extended mRNA is also transcribed beginning in mid-meiosis, regulated by two MSEs (middle sporulation elements), and includes an uORF of 15 codons in its 5'-UTR; evidence transcription is regulated by Pdr1p
6509YPR037CERV2Flavin-linked sulfhydryl oxidase localized to the ER lumenFlavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)
6510YPR038WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci
6511YPR039WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W
6512YPR040WTIP41Protein that interacts with Tap42p, which regulates PP2AProtein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
6513YPR041WTIF5Translation initiation factor eIF5Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
6514YPR042CPUF2PUF family mRNA-binding proteinPUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication
6515YPR043WRPL43ARibosomal 60S subunit protein L43ARibosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
6516YPR044CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO
6517YPR045CTHP3Protein that may have a role in transcription elongationProtein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
6518YPR046WMCM16Component of the Ctf19 complex and the COMA subcomplexComponent of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
6519YPR047WMSF1Mitochondrial phenylalanyl-tRNA synthetaseMitochondrial phenylalanyl-tRNA synthetase; active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
6520YPR048WTAH18Conserved NAPDH-dependent diflavin reductaseConserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance; possible target for development of antifungal drugs
6521YPR049CATG11Adapter protein for pexophagy and the Cvt targeting pathwayAdapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy
6522YPR050CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF MAK3/YPR051W
6523YPR051WMAK3Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT)Catalytic subunit of the NatC type N-terminal acetyltransferase; involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus
6524YPR052CNHP6AHigh-mobility group (HMG) proteinHigh-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
6525YPR053CYPR053CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C
6526YPR054WSMK1Middle sporulation-specific mitogen-activated protein kinase (MAPK)Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p
6527YPR055WSEC8Essential 121 kDa subunit of the exocyst complexEssential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
6528YPR056WTFB4Subunit of TFIIH complexSubunit of TFIIH complex; involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
6529YPR057WBRR1snRNP protein component of spliceosomal snRNPssnRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
6530YPR058WYMC1Secondary mitochondrial inner membrane glycine transporterPutative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; localizes to vacuole in response to H2O2; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
6531YPR059CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
6532YPR060CARO7Chorismate mutaseChorismate mutase; catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
6533YPR061CJID1Probable Hsp40p co-chaperoneProbable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
6534YPR062WFCY1Cytosine deaminaseCytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
6535YPR063CYPR063CER-localized protein of unknown functionER-localized protein of unknown function
6536YPR064WYPR064WPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6537YPR065WROX1Heme-dependent repressor of hypoxic genesHeme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
6538YPR066WUBA3Protein that activates Rub1p (NEDD8) before neddylationProtein that activates Rub1p (NEDD8) before neddylation; acts together with Ula1p; may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
6539YPR067WISA2Protein required for maturation of mitochondrial [4Fe-4S] proteinsProtein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
6540YPR068CHOS1Class I histone deacetylase (HDAC) family memberClass I histone deacetylase (HDAC) family member; deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex
6541YPR069CSPE3Spermidine synthaseSpermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
6542YPR070WMED1Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
6543YPR071WYPR071WPutative membrane proteinPutative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication
6544YPR072WNOT5Component of the CCR4-NOT core complex, involved in mRNA decappingSubunit of CCR4-NOT global transcriptional regulator; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers
6545YPR073CLTP1Protein phosphotyrosine phosphatase of unknown cellular roleProtein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
6546YPR074CTKL1TransketolaseTransketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
6547YPR074W-AHypothetical protein identified by homology
6548YPR075COPY2Integral membrane protein that acts as a membrane anchor for Ste50pIntegral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
6549YPR076WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6550YPR077CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1
6551YPR078CYPR078CPutative protein of unknown functionPutative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
6552YPR079WMRL1Membrane proteinMembrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
6553YPR080WTEF1Translational elongation factor EF-1 alphaTranslational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF1 has a paralog, TEF2, that arose from the whole genome duplication
6554YPR081CGRS2Glycine-tRNA synthetase, not expressed under normal growth conditionsGlycine-tRNA synthetase, not expressed under normal growth conditions; expression is induced under heat, oxidative, pH, or ethanol stress conditions; more stable than the major glycine-tRNA synthetase Grs1p at 37 deg C; GRS2 has a paralog, GRS1, that arose from the whole genome duplication
6555YPR082CDIB117-kDa component of the U4/U6aU5 tri-snRNP17-kDa component of the U4/U6aU5 tri-snRNP; plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
6556YPR083WMDM36Mitochondrial proteinMitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
6557YPR084WYPR084WPutative protein of unknown functionPutative protein of unknown function
6558YPR085CASA1Subunit of the ASTRA complex, involved in chromatin remodelingSubunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
6559YPR086WSUA7Transcription factor TFIIBTranscription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
6560YPR087WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect
6561YPR088CSRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54)Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
6562YPR089WYPR089WProtein of unknown functionProtein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
6563YPR091CNVJ2Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ)Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
6564YPR092WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6565YPR093CASR1Ubiquitin ligase that modifies and regulates RNA Pol IIUbiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
6566YPR094WRDS3Component of the SF3b subcomplex of the U2 snRNPComponent of the SF3b subcomplex of the U2 snRNP; zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
6567YPR095CSYT1Guanine nucleotide exchange factor (GEF) for Arf proteinsGuanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
6568YPR096CYPR096CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments
6569YPR097WYPR097WProtein that contains a PX domain and binds phosphoinositidesProtein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology
6570YPR098CTMH18Protein of unknown functionProtein of unknown function; localized to the mitochondrial outer membrane
6571YPR099CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W
6572YPR100WMRPL51Mitochondrial ribosomal protein of the large subunitMitochondrial ribosomal protein of the large subunit
6573YPR101WSNT309Member of the NineTeen Complex (NTC)Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
6574YPR102CRPL11ARibosomal 60S subunit protein L11ARibosomal 60S subunit protein L11A; expressed at twice the level of Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11A has a paralog, RPL11B, that arose from the whole genome duplication
6575YPR103WPRE2Beta 5 subunit of the 20S proteasomeBeta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome
6576YPR104CFHL1Regulator of ribosomal protein (RP) transcriptionRegulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
6577YPR105CCOG4Essential component of the conserved oligomeric Golgi complexEssential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
6578YPR106WISR1Predicted protein kinasePredicted protein kinase; overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
6579YPR107CYTH1Essential RNA-binding component of cleavage and polyadenylation factorEssential RNA-binding component of cleavage and polyadenylation factor; contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation; relocalizes to the cytosol in response to hypoxia
6580YPR108WRPN7Essential non-ATPase regulatory subunit of the 26S proteasomeEssential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
6581YPR108W-AYPR108W-APutative protein of unknown functionPutative protein of unknown function; identified by fungal homology and RT-PCR
6582YPR109WGLD1Predicted membrane proteinPredicted membrane protein; diploid deletion strain has high budding index
6583YPR110CRPC40RNA polymerase subunit AC40RNA polymerase subunit AC40; common to RNA polymerase I and III
6584YPR111WDBF20Ser/Thr kinase involved in late nuclear divisionSer/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication
6585YPR112CMRD1Essential conserved small ribosomal subunit (40s) synthesis factorEssential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
6586YPR113WPIS1Phosphatidylinositol synthasePhosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
6587YPR114WYPR114WPutative protein of unknown functionPutative protein of unknown function
6588YPR115WRGC1Putative regulator of the Fps1p glycerol channelPleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication
6589YPR116WRRG8Protein of unknown functionPutative protein of unknown function; required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport
6590YPR117WYPR117WPutative protein of unknown functionPutative protein of unknown function
6591YPR118WMRI15'-methylthioribose-1-phosphate isomerase5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
6592YPR119WCLB2B-type cyclin involved in cell cycle progressionB-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
6593YPR120CCLB5B-type cyclin involved in DNA replication during S phaseB-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication
6594YPR121WTHI22Protein with similarity to hydroxymethylpyrimidine phosphate kinasesProtein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis
6595YPR122WAXL1Haploid specific endoprotease of a-factor mating pheromoneHaploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
6596YPR123CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
6597YPR124WCTR1High-affinity copper transporter of plasma membraneHigh-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
6598YPR125WYLH47Mitochondrial inner membrane proteinMitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome
6599YPR126CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6600YPR127WYPR127WPutative pyridoxine 4-dehydrogenasePutative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
6601YPR128CANT1Peroxisomal adenine nucleotide transporterPeroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
6602YPR129WSCD6Repressor of translation initiationRepressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
6603YPR130CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6604YPR131CNAT3Catalytic subunit of the NatB N-terminal acetyltransferaseCatalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
6605YPR132WRPS23BRibosomal protein 28 (rp28) of the small (40S) ribosomal subunitRibosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
6606YPR133CSPN1Protein involved in RNA polymerase II transcriptionProtein involved in RNA polymerase II transcription; is constitutively recruited to the CYC1 promoter and is required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
6607YPR133W-ATOM5Component of the TOM (translocase of outer membrane) complexComponent of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore
6608YPR134WMSS18Nuclear encoded protein needed for splicing of mitochondrial intronNuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
6609YPR135WCTF4Chromatin-associated proteinChromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
6610YPR136CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
6611YPR137C-AYPR137C-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
6612YPR137C-BYPR137C-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
6613YPR137WRRP9Protein involved in pre-rRNA processingProtein involved in pre-rRNA processing; associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
6614YPR138CMEP3Ammonium permease of high capacity and low affinityAmmonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
6615YPR139CLOA1Lysophosphatidic acid acyltransferaseLysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
6616YPR140WTAZ1Lyso-phosphatidylcholine acyltransferaseLyso-phosphatidylcholine acyltransferase; required for normal phospholipid content of mitochondrial membranes; major determinant of the final acyl chain composition of the mitochondrial-specific phospholipid cardiolipin; mutations in human ortholog tafazzin cause Barth syndrome, a rare X-linked disease characterized by skeletal and cardiomyopathy and bouts of cyclic neutropenia
6617YPR141CKAR3Minus-end-directed microtubule motorMinus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
6618YPR142CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
6619YPR143WRRP15Nucleolar proteinNucleolar protein; constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
6620YPR144CNOC4Nucleolar proteinNucleolar protein; forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits; relocalizes to the cytosol in response to hypoxia
6621YPR145C-AYPR145C-APutative protein of unknown functionPutative protein of unknown function
6622YPR145WASN1Asparagine synthetaseAsparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
6623YPR146CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6624YPR147CYPR147CBifunctional triacylglycerol lipase and short chain ester hydrolasePutative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
6625YPR148CYPR148CProtein of unknown functionProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
6626YPR149WNCE102Protein of unknown functionProtein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication
6627YPR150WDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C
6628YPR151CSUE1Protein required for degradation of unstable forms of cytochrome cProtein required for degradation of unstable forms of cytochrome c; located in the mitochondria
6629YPR152CURN1Protein of unknown function containing WW and FF domainsPutative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
6630YPR153WMAY24Protein of unknown functionPutative protein of unknown function
6631YPR154WPIN3Negative regulator of actin nucleation-promoting factor activityNegative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication
6632YPR155CNCA2Protein that regulates expression of Fo-F1 ATP synthase subunitsProtein that regulates expression of Fo-F1 ATP synthase subunits; involved in the regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
6633YPR156CTPO3Polyamine transporter of the major facilitator superfamilyPolyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
6634YPR157WTDA6Putative protein of unknown functionPutative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele; TDA6 has a paralog, VPS62, that arose from the whole genome duplication
6635YPR158C-CYPR158C-CRetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
6636YPR158C-DYPR158C-DRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
6637YPR158WCUR1Sorting factor, central regulator of spatial protein quality controlSorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
6638YPR158W-AYPR158W-ARetrotransposon TYA Gag gene co-transcribed with TYB PolRetrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
6639YPR158W-BYPR158W-BRetrotransposon TYA Gag and TYB Pol genesRetrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
6640YPR159C-AYPR159C-AProtein of unknown functionProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6641YPR159WKRE6Type II integral membrane proteinType II integral membrane protein; required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication
6642YPR160C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6643YPR160WGPH1Glycogen phosphorylase required for the mobilization of glycogenGlycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; expression is regulated by stress-response elements and by the HOG MAP kinase pathway
6644YPR160W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry
6645YPR161CSGV1Cyclin (Bur2p)-dependent protein kinaseCyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins
6646YPR162CORC4Subunit of the origin recognition complex (ORC)Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication
6647YPR163CTIF3Translation initiation factor eIF-4BTranslation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel
6648YPR164WMMS1Subunit of E3 ubiquitin ligase complex involved in replication repairSubunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
6649YPR165WRHO1GTP-binding protein of the rho subfamily of Ras-like proteinsGTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
6650YPR166CMRP2Mitochondrial ribosomal protein of the small subunitMitochondrial ribosomal protein of the small subunit
6651YPR167CMET163'-phosphoadenylsulfate reductase3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism
6652YPR168WNUT2Subunit of the RNA polymerase II mediator complexSubunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
6653YPR169WJIP5Protein required for biogenesis of the large ribosomal subunitProtein required for biogenesis of the large ribosomal subunit; required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein; essential gene
6654YPR169W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps two other dubious ORFs: YPR170C and YPR170W-B
6655YPR170CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORFs YPR169W-A and YPR170W-B
6656YPR170W-ADubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry
6657YPR170W-BYPR170W-BPutative protein of unknown functionPutative protein of unknown function; conserved in fungi; partially overlaps the dubious genes YPR169W-A, YPR170W-A and YRP170C
6658YPR171WBSP1Adapter that links synaptojanins to the cortical actin cytoskeletonAdapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p
6659YPR172WYPR172WProtein of unknown functionPutative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
6660YPR173CVPS4AAA-ATPase involved in multivesicular body (MVB) protein sortingAAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
6661YPR174CYPR174CProtein of unknown functionProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
6662YPR175WDPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon)Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate
6663YPR176CBET2Beta subunit of Type II geranylgeranyltransferaseBeta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
6664YPR177CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
6665YPR178WPRP4Splicing factorSplicing factor; component of the U4/U6-U5 snRNP complex
6666YPR179CHDA3Subunit of the HDA1 histone deacetylase complexSubunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; relocalizes to the cytosol in response to hypoxia; similar to Hda2p
6667YPR180WAOS1Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2pSubunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia
6668YPR181CSEC23GTPase-activating protein, stimulates the GTPase activity of Sar1pGTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
6669YPR182WSMX3Core Sm protein Sm FCore Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
6670YPR183WDPM1Dolichol phosphate mannose (Dol-P-Man) synthase of ER membraneDolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane; catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
6671YPR184WGDB1Glycogen debranching enzymeGlycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
6672YPR185WATG13Regulatory subunit of the Atg1p signaling complexRegulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure
6673YPR186CPZF1Transcription factor IIIA (TFIIIA)Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
6674YPR187WRPO26RNA polymerase subunit ABC23RNA polymerase subunit ABC23; common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
6675YPR188CMLC2Regulatory light chain for the type II myosin Myo1pRegulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
6676YPR189WSKI3Ski complex component and TPR proteinSki complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
6677YPR190CRPC82RNA polymerase III subunit C82RNA polymerase III subunit C82
6678YPR191WQCR2Subunit 2 of ubiquinol cytochrome-c reductase (Complex III)Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme
6679YPR192WAQY1Spore-specific water channelSpore-specific water channel; mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance
6680YPR193CHPA2Tetrameric histone acetyltransferaseTetrameric histone acetyltransferase; has similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; also acetylates polyamines
6681YPR194COPT2Oligopeptide transporterOligopeptide transporter; localized to peroxisomes and affects glutathione redox homeostasis; also localizes to the plasma membrane (PM) and to the late Golgi, and has a role in maintenance of lipid asymmetry between the inner and outer leaflets of the PM; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles and in polarized cell growth
6682YPR195CYPR195CPutative protein of unknown functionDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
6683YPR196WYPR196WPutative maltose-responsive transcription factorPutative maltose-responsive transcription factor
6684YPR197CDubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SGE1/YPR198W
6685YPR198WSGE1Plasma membrane multidrug transporterPlasma membrane multidrug transporter; member of the major facilitator superfamily; acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
6686YPR199CARR1Transcriptional activator of the basic leucine zipper (bZIP) familyTranscriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds
6687YPR200CARR2Arsenate reductase required for arsenate resistanceArsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p
6688YPR201WARR3Plasma membrane metalloid/H+ antiporterPlasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
6689YPR202WYPR202WPutative protein of unknown functionPutative protein of unknown function; similar to telomere-encoded helicases; down-regulated at low calcium levels; YPR202W is not an essential gene; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
6690YPR203WYPR203WPutative protein of unknown functionPutative protein of unknown function
6691YPR204C-AProtein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
6692YPR204WYPR204WDNA helicase encoded within the telomeric Y' elementDNA helicase encoded within the telomeric Y' element; Y' -helicase protein 1